ライフサイエンスコーパス: pipette solution

1 1 mM N-ethylmaleimide, respectively, to the pipette solution.

2 activity when GTP and ATP were added to the pipette solution.

3 ophilic fluorescent dyes are included in the pipette solution.

4 3X, 3 microM) or by omission of ATP from the pipette solution.

5 acellular Cl- concentration unaltered by the pipette solution.

6 ing the driving force by modifying the patch-pipette solution.

7 hotolysing caged GTP-gamma-S included in the pipette solution.

8 and eliminated in the absence of ATP in the pipette solution.

9 te, whereas others used an ATP- and GTP-free pipette solution.

10 ut not other phosphoinositides, to the patch pipette solution.

11 the use of amphotericin B with a high [Cl-] pipette solution.

12 trical activity irrespective of [Cl-] in the pipette solution.

13 e, or a calmodulin inhibitory peptide in the pipette solution.

14 ng guanosine-5'-O-(2-thiodiphosphate) in the pipette solution.

15 hen a weak Mg(2+) chelator is present in the pipette solution.

16 on, ATP at millimolar concentrations in the pipette solution.

17 ce in the cytoplasm is slowly diluted by the pipette solution.

18 dye from the cytoplasm with a dye-free patch pipette solution.

19 ated CaMKII inhibitory peptide (ARIP) in the pipette solution.

20 ate represented the only Ca2+ buffers in the pipette solution.

21 ould be reduced by inclusion of NH4Cl in the pipette solution.

22 mide (QX-314) was omitted from the recording pipette solution.

23 rnal solution or 5 mM BAPTA was added to the pipette solution.

24 rapid calcium chelator BAPTA (11 mM) in the pipette solution.

25 inclusion of a PKA inhibitor peptide in the pipette solution.

26 or by intracellular dialysis with low Ca(2+) pipette solutions.

27 2+ current in the presence of Cs+-containing pipette solutions.

28 With Ba2+ in the pipette solution (10 mmol.L(-1)), patch depolarization w

29 ml-1 protein kinase A inhibitor (PKI) to the pipette solution, 10 microM ISO enhanced INa by 30.5 +/-

30 ith CsCl, when organic ions were used in the pipette solution a significantly higher proportion of lo

31 When 2 microM annexin IV was present in the pipette solution, a concentration that by itself has no

32 GDP beta S (1 mM), included in the pipette solution, abolished the inhibition by Ang II, wh

33 t caveolin-3 to the cell's cytoplasm via the pipette solution abrogated this direct G protein-induced

34 resence of tyrphostin B42 (10 microM) in the pipette solution activated tolbutamide-sensitive KATP ch

35 kinase (protein kinase A, PKA), added to the pipette solution, activated equivalent currents in five

36 alpha5alphaP is normally applied through the pipette solution, addition of steroid to the bath soluti

37 p at 30-35 degrees C, using standard K+-rich pipette solution and external solutions containing 11.1

38 KC inhibitor (PKCI19-36) was included in the pipette solution and mimicked by the extracellular appli

39 ent was eliminated by removal of K+ from the pipette solution and partially blocked by the TASK (tand

40 ssium-free conditions, in both the dialysing pipette solution and perfusing saline, NMC was still abl

41 To reap the benefit of seal-promoting pipette solutions and yet retain the option to adjust th

42 ump) (both at 10 and 100 mmol/L Na(+) in the pipette solution) and maximal NKA-mediated Na(+) extrusi

43 ct of replacing K+ with Cs+ in the dialysing pipette solution, and the effect of altering dialysing [

44 l/L), was suppressed by ATP removal from the pipette solution, and was blocked by PKC inhibitors chel

45 against intracellular epitopes, in the patch pipette solution blocked TRPC1/C5 channel currents but p

46 In cells dialysed with pipette solution buffered to pH 7.2, step changes in pHo

47 Patch pipette solutions buffered with 1-4 mm of either BAPTA o

48 Na(+)](o) to 10 mm, the concentration in the pipette solution, by substitution with NMDG(+) shifted V

49 tly upon application of AP while a Ca2+-free pipette solution completely abolished the electrical res

50 caffolding domain peptide (10 microM) in the pipette solution completely abrogated the effects of ISO

51 c anti-Gs alpha antibody (100 nmol/L) in the pipette solution completely blocked the activation of th

52 ing K+ current (IK(IR)) was prevented if the pipette solution contained Mg-ATP.

53 +/- 5.3% (n = 13, P < 0.05 vs. control) with pipette solution containing 1 microM Gsalpha27-42 peptid

54 Internal perfusion of cells with a pipette solution containing ATPgammaS was used to preven

55 With the pipette solution containing both PKI and 20 microgram ml

56 When a pipette solution containing EGTA and Mg2+ was used to bu

57 With a pipette solution containing gramicidin, which forms Cl--

58 stabilizes when cells are patch clamped with pipette solutions containing 10 mm BAPTA and free Ca2+ c

59 Pipette solutions containing 10 mM BAPTA and no added AT

60 tivity could be evoked by 16 mM glucose with pipette solutions containing 80 or 150 mM Cl-.

61 cium ionophore ionomycin or by dialysis with pipette solutions containing buffered elevated [Ca2+] pr

62 hole-cell current densities are similar with pipette solutions containing cesium, potassium, or sodiu

63 Replacement of pipette solutions containing CsCl with solutions contain

64 dialysed and superfused with K+ -free media, pipette solutions containing fixed levels of free Ca(2+)

65 In PV myocytes, I(Cl(Ca)) was evoked by pipette solutions containing up to 1 microM free Ca(2+)

66 with a steeper dependence on voltage if the pipette solution contains K(+) as the main cation than i

67 as high and elimination of it with K(+)-free pipette solution could not be reconciled with restricted

68 ol 1,4,5-trisphosphate (Ins(1,4,5)P3) in the pipette solution did not evoke Icat but greatly potentia

69 nhibitor calmidazolium via the intracellular pipette solution did not inhibit rapid endocytosis.

70 on, calcium ionophore, or calcium-containing pipette solutions did not cause exocytosis.

71 of the Mg2+ chelators EDTA and ATP in 0 Mg2+ pipette solutions did not increase the whole-cell curren

72 rotons by buffering HC cytosol with a pH 9.2 pipette solution eliminated feedback, whereas alkalinizi

73 Including 30 mM Hepes in the pipette solution eliminated the effects of quinine and N

74 s of the fibres with 20 mM EGTA in the patch pipette solution eliminated the tail current, consistent

75 When 2 mM GDP-beta-S was added in pipette solution, ethanol-induced potentiation of I(Gly)

76 eotides, swelling of cells with a hypertonic pipette solution failed to activate the conductance.

77 Addition of purified PKC to the pipette solution, followed by a pretreatment with TPA, r

78 When added to the pipette solution, H-89, a PKA inhibitor, blocked ATP and

79 and the K+ and the Cl(-)concentration of the pipette solution had an effect on the seal formation.

80 racellular action because KYNA (1 mm) in the pipette solution had no effect on alpha7 nAChR activity.

81 Inclusion of NFA in the pipette solution had no effect on I(Cl(Ca)).

82 Perfusion of ADP or control pipette solution had no effect, whereas perfusion of ATP

83 of Ins(3,4,5,6)P4 (1 microM) present in the pipette solution had no significant effect on Cl- curren

84 In ruptured-patch recording using Ca2+-free pipette solution, Ileak was strongly enhanced, and was i

85 clamping cytosolic Ca(2+) concentration with pipette solution in which Ca(2+) was buffered to 1 micro

86 robustly inhibits ClC-1 when included in the pipette solution in whole cell patch clamp experiments a

87 ot affected by 10-15 microM free Ca2+ in the pipette solution, inactivation of Ca2+ currents during d

88 i) and cytosolic [Ca(2+)] ([Ca(2+)](i)), the pipette solution included 30 mM citrate and 10 mM ATP al

89 purified catalytic subunit Calpha-PKA in the pipette solution increased neuronal AMPA receptor P(O,PE

90 Inclusion of 1 mum Ins(1,4,5)P3 in the patch pipette solution increased whole-cell currents evoked by

91 Acidification of the pipette solution (increasing [H+]i) augmented the outwar

92 uced (72%) with 200 microM GDP-beta-S in the pipette solution, indicating that it is a postsynaptic p

93 um through inclusion of 750 nm Ca(2+) in the pipette solution, indicating that neither the calcium se

94 Inclusion of Ac-10C in the dialysing pipette solution inhibited resting Ca(2+) spark frequenc

95 ddition of the anti-Pgp antibody C219 to the pipette solution inhibited this current by 75% only in t

96 When Ca2+ was present only in the pipette solution, just the focal change in [Ca2+]i was o

97 When ATP was included in the pipette solution, MCC-134 slowly activated surface K(ATP

98 II (CaMKII) inhibitor peptides in the patch pipette solutions not only blocked desensitization of 'o

99 (2-thiodiphosphate)] (2 mM) was added to the pipette solution of whole-cell recordings to regulate G

100 When Ca2+ buffer was omitted from the patch pipette solution, 'on' bipolar cells rapidly desensitize

101 -thiodiphosphate) in the intracellular patch pipette solution or by pretreatment with pertussis toxin

102 Addition of BAPTA (10 mM) to the pipette solution or replacement of extracellular Ca2+ wi

103 nce was unaffected by exposure to anisotonic pipette solutions or to increases in intracellular cAMP;

104 With high levels of calcium chelators in the pipette solution, or bath application of bicuculline, EP

105 of it when the Na(+) concentration in patch pipette solutions perfusing the intracellular compartmen

106 Recordings made with an ATP- and GTP-free pipette solution produced large and robust TRPC5 current

107 anti-PIP2 monoclonal antibody in whole cell pipette solution produced the opposite effects of PIP2.

108 ion of 1 mM L-NMMA + 1 mM L-NNA in the patch pipette solution) produced no significant attenuation of

109 of a CaMKII inhibitory peptide in the patch-pipette solution removed the rectification even in the p

110 oxy)ethane-N,N,N',N'-tetraacetic acid in the pipette solution, showed 50% inactivation in <5 min and

111 With the Ca2+ chelator BAPTA in the patch pipette solution, step responses of 'on' bipolar cells w

112 ors and after removal of ATP or GTP from the pipette solution, suggesting a cGMP-independent signalli

113 on of the G-protein subunit G(o)alpha to the pipette solution suppressed the cation current and occlu

114 With intracellular pipette solutions that controlled free [Ca(2+) ]i , we f

115 e cell patch clamp recordings were made with pipette solutions that support activation of both Ca2+-

116 When EGTA was omitted from the pipette solution, the number of sparks triggered in KO a

117 When added to the internal pipette solution, the protein kinase A inhibitor KT 5720

118 Buffering Ca2+ in the patch pipette solution to 1 microM prevented desensitization,

119 T cells with 200 muM Navbeta4 peptide in the pipette solution to induce resurgent sodium currents.

120 Currents were recorded using Cs+-rich pipette solutions to block K+ currents.

121 ent when we included glutaredoxin 1 in patch pipette solutions to reverse glutathionylation.

122 ith 140 mM KCl and 1 mM Mg2+ in the bath and pipette solutions, two main open levels with conductance

123 rat beta-cells by varying [Cl-] in the patch pipette solution using the Cl--permeable antibiotic amph

124 ation as dialysis with nominally Mg(2+)-free pipette solution was associated with an increase in the

125 ole-cell and mean patch currents with 0 Mg2+ pipette solution was not due to block of whole-cell curr

126 ular calcium concentrations ([Ca2+]i) to the pipette solution were studied in rabbit pulmonary artery

127 was reproduced by microcystin (10 microM in pipette solution), which is a membrane-impermeant inhibi

128 gating were observed using MgATP or K2ATP in pipette solutions, which increases or decreases [Mg(2+)]

129 rst, when currents were recorded in a 0 Mg2+ pipette solution, whole-cell currents at positive voltag

130 Increasing pH buffering capacity in the pipette solution with 40 mm HEPES attenuated the effects