ライフサイエンスコーパス: planarian

1 gly similar to those guiding regeneration in planarians.

2 arkers and the strong negative phototaxis of planarians.

3 t allows us to follow stem cell migration in planarians.

4 imited regeneration capacities of freshwater planarians.

5 uced genes during regeneration initiation in planarians.

6 the only proliferative cell type in asexual planarians.

7 ings suggest two distinct roles for TORC1 in planarians.

8 lie the remarkable regenerative abilities of planarians.

9 persisting regionalized expression in adult planarians.

10 mechanisms regulate germ cell development in planarians.

11 and axial polarity of regenerated tissue in planarians.

12 We characterized the Hh pathway in planarians.

13 uninjured regions of intact and regenerating planarians.

14 teria and specifies regeneration polarity in planarians.

15 ntity during regeneration and homeostasis in planarians.

16 op, regenerate, or maintain gonads in sexual planarians.

17 f neural precursors at the midline in intact planarians.

18 ect evidence of a 'withdrawal phenomenon' in planarians.

19 ed as absent in free-living flatworms, e.g., planarians.

20 or male GSC specification and maintenance in planarians.

21 homeostasis and post-injury regeneration in planarians.

22 , and is up-regulated during regeneration in planarians.

23 In planarians, a pluripotent stem cell population and perpe

24 rved TALE class protein PBX/Extradenticle in planarians, a representative member of the Lophotrocozoa

25 ome-wide expression profiling of pluripotent planarian adult stem cells (pASCs), Onal et al present e

26 We now report that planarians also display dose-related abstinence-induced

27 We determined that planarian and vertebrate excretory cells express homolog

28 es of stem cell and regeneration dynamics in planarians and Hydra.

29 unction of nanos in germ cell development in planarians and suggest that these animals will serve as

30 ng-lived free-living flatworms (for example, planarians), and neoblast-like cells have been described

31 yet sporadic and include some sponge, hydra, planarian, and salamander (i.e., newt and axolotl) speci

32 (hesl-3) and sim label progenitors in intact planarians, and following amputation we observed an enri

33 expression enriched in sexually reproducing planarians, and identified an orphan chemoreceptor famil

34 onic brains of polychaete annelids, polyclad planarians, and nemerteans.

35 Here, we show that planarians, and possibly other flatworms, lack centrosom

36 Freshwater planarians are able to regenerate any missing part of th

37 Planarians are able to regenerate from essentially any t

38 Freshwater planarians are an attractive model organism for research

39 ional regulatory mechanisms and suggest that planarians are an ideal model for this understudied aspe

40 Planarians are an important model organism for regenerat

41 The regenerative abilities of freshwater planarians are based on neoblasts, stem cells maintained

42 Planarians are capable of regenerating any missing body

43 After amputation, planarians are capable of regenerating new anterior and

44 Planarians are capable of whole-body regeneration and mo

45 Planarians are flatworms and regenerate from tiny body f

46 Planarians are flatworms capable of regenerating all bod

47 Planarians are flatworms capable of regenerating any mis

48 Planarians are flatworms that constitutively maintain ad

49 Planarians are flatworms with robust regenerative capaci

50 Planarians are free-living flatworms capable of rapidly

51 in of animal excretory systems and establish planarians as a novel and experimentally accessible inve

52 on or reproduction by fission, and establish planarians as a pertinent model for studying telomere st

53 on in understudied organisms and establishes planarians as a powerful model for the molecular genetic

54 gene controlling phagocytosis and establish planarians as a powerful system for analyzing host-patho

55 arity determinant notum Our work establishes planarians as a suitable model for further in-depth stud

56 Our results establish planarians as an experimentally tractable animal model f

57 standard techniques, allowing for imaging of planarians at sub-cellular resolution in vivo using brig

58 Further, planarian Bcl-2 family proteins can induce and/or regula

59 with the last common ancestor of acoels and planarians being the ancestor of the Bilateria.

60 We identified 44 planarian bHLH homologs, determined their patterns of ex

61 y dynamic and reveals fundamental aspects of planarian biology that have been previously unappreciate

62 We identified a regional switch in the adult planarian body upon systemic disruption of homologous re

63 ession map exists for the maintenance of the planarian body.

64 alyses indicate that premeiotic functions of planarian boule2 and vertebrate Dazl evolved independent

65 se microRNAs in neuronal organization during planarian brain regeneration.

66 e role of the miR-124 family of microRNAs in planarian brain regeneration.

67 We performed RNA sequencing of planarian brain tissue following RNAi of hh and patched

68 findings indicate that, after decapitation, planarians build an organizing center from stem cells at

69 vidence of asymmetric stem cell divisions in planarians, but also demonstrate that EGF signaling like

70 maintaining and resetting axial polarity in planarians, but it is unclear how planarians reestablish

71 Planarians can be cut into irregularly shaped fragments

72 Because freshwater planarians can regenerate a functional central nervous s

73 Planarians can regenerate any missing body part, requiri

74 e collectively expressed in a broad range of planarian cell types, SL3 is highly enriched in a subset

75 use single-cell transcriptome sequencing on planarian cells to investigate the cell-type specificity

76 In planarians, centrioles are only assembled in terminally

77 tion, and identify additional components, of planarian chromatoid bodies.

78 s; however, these proteins are essential for planarian ciliogenesis.

79 ized, for the first time, glial cells in the planarian CNS that respond to injury by repressing sever

80 GABAergic, and octopaminergic neurons in the planarian CNS.

81 me sequence accessible to the biomedical and planarian communities, we have created the Schmidtea med

82 ell dynamics demonstrates the utility of the planarian digestive system as a model for elucidating th

83 n and define the major categories of defects planarians display following gene perturbations.

84 Planarians display remarkable plasticity in maintenance

85 idered to have a centralized nervous system, planarians, display both abstinence-induced and antagoni

86 We previously reported that planarians (Dugesia dorotocephala) that have been expose

87 We used the planarian epidermal lineage to study how the location of

88 Our results further establish the planarian epidermis as a novel paradigm to uncover the m

89 The planarian epidermis is a simple tissue that undergoes ra

90 gical turnover, injury, and for some such as planarians, even amputation.

91 etiology is remarkably conserved between the planarian excretory system and the vertebrate nephron.

92 Here we focus on the planarian excretory system, which consists of internal p

93 Although planarians experienced a brief fall from grace, with the

94 Planarians famously can regenerate after decapitation.

95 Here, we use the planarian flatworm as a simple chemical-genetic screenin

96 mprehensive model for regeneration, with the planarian flatworm being one of the most important model

97 The planarian flatworm is an emerging model that is useful f

98 enesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterrane

99 sts contain pluripotent stem cells and drive planarian flatworm regeneration from diverse injuries.

100 to producing an ommochrome body pigment, the planarian flatworm Schmidtea mediterranea generates porp

101 zed the spatial expression of SL RNAs in the planarian flatworm Schmidtea mediterranea, with the goal

102 rly unlimited regenerative capabilities make planarian flatworms an ideal system with which to invest

103 The well-known regenerative abilities of planarian flatworms are attributed to a population of ad

104 Planarian flatworms can regenerate heads at anterior-fac

105 Planarian flatworms contain a population of adult stem c

106 a previously unknown prey item (soft-bodied planarian flatworms in the genus Dugesia) made up the ma

107 Planarian flatworms regenerate every organ after amputat

108 Indeed, planarian flatworms were used as experimental models dec

109 Here we study the telomere biology of planarian flatworms with apparently limitless regenerati

110 ed the transcriptomes of major cell types of planarians--flatworms that regenerate from nearly any in

111 erentiate peptide dynamics in the freshwater planarian flatwormSchmidtea mediterraneaat different tim

112 Here we find that a planarian Follistatin homolog directs regeneration of an

113 ody plan and cell fate during embryogenesis, planarian Follistatin promotes reestablishment of anteri

114 ip (PIC) for rapid, stable immobilization of planarians for in vivo imaging without injury or biochem

115 hese difficulties, we present here Planform (Planarian formalization), a manually curated database an

116 iverse organisms, in particular neoblasts of planarians (free-living relatives of schistosomes).

117 We identified a planarian gene, Smed-betacatenin-1, that controls regene

118 ilized transcriptional profiling to identify planarian genes expressed in adult proliferating, regene

119 ortal all available data associated with the planarian genome, including predicted and annotated gene

120 e protein families that are missing from the planarian genome.

121 This plasticity is also exhibited by the planarian germ cell lineage.

122 ting that inductive signaling is involved in planarian germ cell specification.

123 lls are specified by localized determinants, planarian germ cells appear to be specified epigenetical

124 To study the development and regeneration of planarian germ cells, we have functionally characterized

125 Planarian glia and their regulation by Hedgehog signalin

126 Planarian glia were distributed broadly, but only expres

127 We propose that these cells are planarian glia.

128 and, therefore, defines a secondary role for planarian gonadal niche cells in promoting GSC different

129 receptor mining, we identified 566 putative planarian GPCRs and classified them into conserved and p

130 Our studies uncover the complement of planarian GPCRs and reveal previously unappreciated role

131 Planarians grow and regenerate organs by coordinating pr

132 ntrast to most well-studied model organisms, planarians have a remarkable ability to completely regen

133 of their exceptional regenerative abilities, planarians have become important models for understandin

134 posterior Wnt expression domains, controlled planarian head and trunk patterning.

135 A cell cluster at the anterior tip of planarian head blastemas (the anterior pole) is required

136 These very same neurons also produce the planarian hedgehog ligand (Smed-hh), which appears to co

137 evealing an important mitogenic role for the planarian hh signaling molecule in the adult CNS.

138 We have identified two planarian homeobox transcription factors, Smed-nkx2.1 an

139 SmedOB1 in planarian, which is required for planarian homeostasis and regeneration.

140 main epithelial progenitor populations and a planarian homolog to the MEX3 RNA-binding protein (Smed-

141 l, we identify and functionally characterize planarian homologs of human DAZL/DAZ-interacting partner

142 ic eukaryote organisms including mosquitoes, planarians, human and other mammalian cells, and the mal

143 We have developed the Planarian Immobilization Chip (PIC) for rapid, stable im

144 , which eliminates detectable Bruli protein, planarians initiate the proliferative response to amputa

145 nthesized in vitro We identified one of four planarian integrin-alpha subunits inhibition of which ph

146 s end, we analyzed the expression profile of planarian intestinal phagocytes, cells responsible for d

147 indicate that growth and regeneration of the planarian intestine are achieved by co-ordinated differe

148 have analyzed growth and regeneration of the planarian intestine, the organ responsible for digestion

149 The asexual freshwater planarian is a constitutive adult, whose central nervous

150 ipulated and studied in vivo, the freshwater planarian is an ideal system with which to investigate t

151 Brain regeneration in planarians is mediated by precise spatiotemporal control

152 ne and amphetamine, but not cannabinoids, in planarians is mediated through a common nor-BNI-sensitiv

153 xperiments from the main publications in the planarian literature.

154 n functional regeneration experiments in the planarian literature; By analyzing all the datasets toge

155 ns of SmedOB1 provide one mechanism by which planarians maintain telomere and genome stability to ens

156 a reciprocal manner with SLIT to pattern the planarian mediolateral axis, while WNT11-2 patterns the

157 Smed-slit is expressed along the planarian midline, in both dorsal and ventral domains.

158 Using the planarian model system, we report that membrane voltage-

159 ntal pathways are predominantly expressed in planarian muscle cells.

160 tting and find unexpected roles for distinct planarian muscle fibers.

161 Most PCGs are expressed within planarian muscle; however, how muscle is specified and h

162 Planarian neoblasts are pluripotent, adult somatic stem

163 These findings indicate that planarian neoblasts comprise two major and functionally

164 These cells resemble planarian neoblasts morphologically and share their abil

165 We show that regenerating planarians' normal anterior-posterior pattern can be per

166 nd laterally in adult Schmidtea mediterranea planarians, opposing the dorsal-pole expression of Smed-

167 We find that either planarian or human TRPA1 can restore noxious-heat avoida

168 tected in the vast majority of newly hatched planarians or in small tissue fragments that will ultima

169 RNA interference (RNAi) knockdown of a planarian ortholog of the axon-guidance receptor roundab

170 RNA-mediated genetic interference (RNAi) of planarian orthologues of human CKD genes and inhibition

171 tative analysis, we showed that after injury planarians perfectly restored brain:body proportion by i

172 regeneration assay in which ejection of the planarian pharynx is selectively induced by brief exposu

173 The planarian PIWI homologs SMEDWI-1 and SMEDWI-3 are requir

174 s directly controls stem cell-specific AS in planarians, placing the origin of this regulatory mechan

175 In planarians, pluripotent somatic stem cells called neobla

176 In planarians, positional control genes (PCGs) control rege

177 In planarians, positional information has been identified f

178 y be conserved and is an instructive part of planarian posterior regeneration.

179 Head-versus-tail regeneration in planarians presents a paradigm for study of this phenome

180 RNA interference-mediated depletion of planarian PRMT5 results in defects in homeostasis and re

181 After amputation, freshwater planarians properly regenerate a head or tail from the r

182 on regulatory proteins that are required for planarian protonephridia regeneration.

183 Overall, our characterization of the planarian protonephridial system establishes a new parad

184 (frog, fish, mouse, and amphioxus) and from planarians (protostomes) suggest that Wnt signaling thro

185 Thus, planarians provide a powerful model to identify genes re

186 The remarkable plasticity of freshwater planarians provides the opportunity to study these molec

187 olarity in planarians, but it is unclear how planarians reestablish polarity signaling centers after

188 Planarians regenerate all body parts after injury, inclu

189 ally, we explored genes downregulated during planarian regeneration and characterized, for the first

190 milar to CHD4/Mi-2 proteins, as required for planarian regeneration and tissue homeostasis.

191 For example, planarian regeneration has been studied for over a centu

192 Planarian regeneration involves regionalized gene expres

193 possibility that an important early step in planarian regeneration is the specialization of neoblast

194 Planarian regeneration requires adult stem cells called

195 Planarian regeneration requires neoblasts, a population

196 erse-engineer the first mechanistic model of planarian regeneration that can recapitulate the main an

197 pable of searching for and finding models of planarian regeneration that match experimental data stor

198 Planarian regeneration uses a population of regenerative

199 In planarian regeneration, new cells arise from a prolifera

200 ts suggest that hnf4 is a regulatory gene in planarian regeneration, validate the computational predi

201 de automated searches for unbiased models of planarian regeneration.

202 nd we demonstrate a broad requirement during planarian regeneration.

203 atin homolog (Smed-follistatin) required for planarian regeneration.

204 ns, we present a different kind of review of planarian regeneration.

205 expression of the Innexin gene family during planarian regeneration.

206 t by the reverse-engineered dynamic model of planarian regeneration.

207 the conserved JNK signalling cascade during planarian regeneration.

208 le in specifying posterior cell fates during planarian regeneration.

209 ive dynamical model explaining patterning in planarian regeneration.

210 In planarians, regeneration of entire animals from tissue f

211 ually curated database and software tool for planarian regenerative experiments, based on a mathemati

212 e developed a linear mechanical model with a planarian represented by a thin shell.

213 Asexual freshwater planarians reproduce by tearing themselves into two piec

214 tify a role for nuclear hormone receptors in planarian reproductive maturation and reinforce the sign

215 ns, SmedGD will prove useful not only to the planarian research community, but also to those engaged

216 This procedure will be useful for all planarian researchers, particularly those with relativel

217 our results demonstrate that where and how a planarian rips itself apart during asexual reproduction

218 micals elicit robust escape behaviors in the planarian Schmidtea mediterranea and that the conserved

219 ave used MAKER to annotate the genome of the planarian Schmidtea mediterranea and to create a new gen

220 Using the planarian Schmidtea mediterranea as a model, we identify

221 RNA interference of Smed-iguana in the planarian Schmidtea mediterranea caused cilia loss and f

222 The planarian Schmidtea mediterranea is rapidly emerging as

223 The planarian Schmidtea mediterranea is well suited for inve

224 dentify two boule paralogs in the freshwater planarian Schmidtea mediterranea Smed-boule1 is necessar

225 ashirt family of zinc-finger proteins in the planarian Schmidtea mediterranea to be a target of Wnt s

226 Here we used the ciliated epithelium of the planarian Schmidtea mediterranea to dissect the role of

227 enome, and RNAi in the sexual biotype of the planarian Schmidtea mediterranea to test that hypothesis

228 rin-producing cells in the brown, freshwater planarian Schmidtea mediterranea Using an RNA-sequencing

229 In the planarian Schmidtea mediterranea, hedgehog (hh) is expre

230 An unlikely model animal, the planarian Schmidtea mediterranea, is proving valuable in

231 We found that in the planarian Schmidtea mediterranea, RNA interference (RNAi

232 m cells in a simultaneous hermaphrodite, the planarian Schmidtea mediterranea.

233 members of the Wnt signaling pathway in the planarian Schmidtea mediterranea.

234 e study of regeneration initiation using the planarian Schmidtea mediterranea.

235 tem cell division during regeneration in the planarian Schmidtea mediterranea.

236 upials), one amphibian and one flatworm, the planarian Schmidtea mediterranea.

237 of the Slit family of guidance cues from the planarian Schmidtea mediterranea.

238 d in the adult stem cells (neoblasts) of the planarian Schmidtea mediterranea.

239 ogenesis during anterior regeneration in the planarian Schmidtea mediterranea.

240 is critical for regulating neoblasts in the planarian Schmidtea mediterranea.

241 dividing adult stem cells (neoblasts) of the planarian Schmidtea mediterranea.

242 ful in vivo model for stem cell biology, the planarian Schmidtea mediterranea.

243 regulatory roles during regeneration in the planarian Schmidtea mediterranea.

244 own to regulate germ cell development in the planarian Schmidtea mediterranea; thus, we sought to inv

245 Freshwater planarians serve as fascinating models for studying thes

246 ons to work on neuropeptidergic signaling in planarian showed interesting parallels but also remarkab

247 cause of the unique phylogenetic position of planarians, SmedGD will prove useful not only to the pla

248 However, planarian species with limited regenerative abilities ar

249 ter Smed-nanos RNAi, suggesting that asexual planarians specify germ cells, but their differentiation

250 ollowed by full-length homologous pairing in planarian spermatocytes, is not observed in other specie

251 gnificant (P < 0.05) decrease in the rate of planarian spontaneous locomotor activity over a 5-min ob

252 types and identify genetic regulators of the planarian stem cell system.

253 Asexual adult planarian stem cells appear to maintain telomere length

254 During regeneration, planarian stem cells are induced to form a cell populati

255 Here, we show that planarian stem cells directionally migrate to amputation

256 s, SL3 is highly enriched in a subset of the planarian stem cells engaged in regenerative responses.

257 (2016) show that a PIWI protein expressed in planarian stem cells is inherited by their differentiati

258 generation in which eye tissue production by planarian stem cells is not directly regulated by the ab

259 Planarian stem cells nearby the brain express core hh si

260 Our data reveal that the regulation of planarian stem cells relies on posttranscriptional regul

261 nown about the extrinsic signals that act on planarian stem cells to modulate rates of neurogenesis.

262 These findings suggest that planarian stem cells utilize molecular mechanisms found

263 s of chromatoid bodies and their function in planarian stem cells, and also support emerging studies

264 n uninjured organs, occurs in the absence of planarian stem cells, and can also be triggered by prolo

265 introns that is differentially regulated in planarian stem cells, and comprehensively identify its r

266 Planarian stem cells, or neoblasts, drive the almost unl

267 omatoid bodies to histone mRNA regulation in planarian stem cells.

268 Because planarians stop "doing it" at the slightest disturbance,

269 m grace, with the advent of molecular tools, planarians, such as Schmidtea mediterranea, have emerged

270 or Hofstenia muscle and this similarity with planarians suggests mesodermal muscle originated at the

271 scale gene inhibition studies to the classic planarian system.

272 -pbx (pbx for short), which encodes a second planarian TALE-family homeodomain transcription factor,

273 We present a cell-based model of planarian that can regenerate anatomical regions followi

274 Neoblasts are adult stem cells (ASCs) in planarians that sustain cell replacement during homeosta

275 In intact and regenerating planarians, the regulation of Wnt/beta-catenin signaling

276 Planarians thus present a fertile ground for the identif

277 he core components of the TOR pathway during planarian tissue homeostasis and regeneration and identi

278 MS ion images of planarian tissue sections allow changes in peptides and

279 ls (ASCs) that facilitate the maintenance of planarian tissues and organs, providing a powerful syste

280 nsduction, demonstrate its conservation from planarians to humans, and imply that animal nociceptive

281 ere we used functional genomic approaches in planarians to identify genes required for proper germ ce

282 and Smed-netrin2 are also required in intact planarians to maintain the proper patterning of the CNS.

283 revious work provided indirect evidence that planarians undergo abstinence-induced withdrawal from co

284 Planarians undergo whole-body regeneration and tissue tu

285 Planarians use Wnt/beta-catenin signaling to polarize th

286 Characterization of the entire family of planarian voltage-operated Ca(2+) channel alpha subunits

287 Planarians, well known for their ability to undergo whol

288 oreover, DNA damage and apoptosis signals in planarian were significantly affected by SmedOB1 RNAi.

289 Planarians were soaked in cocaine then transferred to ei

290 novel telomere-associated protein SmedOB1 in planarian, which is required for planarian homeostasis a

291 ersus-tail regeneration polarity decision in planarians, which requires Wnt signaling, provides a par

292 initial mechanistic studies of apoptosis in planarians, which revealed that a S. mediterranea homolo

293 These findings suggest that studies of planarians will inform our understanding of germ cell bi

294 Here we examine Procotyla fluviatilis, a planarian with restricted ability to replace missing tis

295 g of the remarkable regeneration capacity of planarian worms and demonstrate the power of this automa

296 e same surgical and genetic experiments with planarian worms, obtaining the same phenotypic outcomes

297 the PIC by performing time-lapse imaging of planarian wound closure and sequential imaging over days

298 Most planarian wound-induced genes are conserved across metaz

299 The gene activation program that occurs at planarian wounds to coordinate regenerative responses re

300 uality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.