ライフサイエンスコーパス: plasmid

1 sed with expression of the pCMVbeta reporter plasmid.

2 trol microbe which solely harbored the pNZ44 plasmid.

3 to the chromosome and bla(VIM-1) to a 58-kb plasmid.

4 lated by GODZ using a GODZ dominant-negative plasmid.

5 ticles upon transfection with a GFP replicon plasmid.

6 une system that protects against viruses and plasmids.

7 formation within negatively supercoiled DNA plasmids.

8 ainst invasive nucleic acids from phages and plasmids.

9 host that is at least as high as the cost of plasmids.

10 oups and mixed populations carrying multiple plasmids.

11 rmation of non-covalent adducts with natural plasmids.

12 romosomal DNA repair and replication of some plasmids.

13 eukaryotic DNA viruses and self-replicating plasmids.

14 of Gram-negative microbes carrying different plasmids.

15 mors after hydrodynamic gene delivery of AKT plasmids.

16 for bacteria and archaea against viruses and plasmids.

17 ircular chromosomes and two smaller putative plasmids.

18 hogenesis are located on two large virulence plasmids.

19 ly segregate chromosomes and low copy-number plasmids.

20 The toolkit includes 23 Cas9-sgRNA plasmids, 37 promoters of various strengths and temporal

21 atibility types, the IncI2, IncX4 and IncHI2 plasmids accounted for more than 90% of MGEs carrying th

22 ion ensures that both daughter cells inherit plasmids after cell division.

23 Sequence analysis of the circularized plasmids allowed measurement of relative activity of MME

24 We screened IncX4 plasmids among 2,470 isolates of Enterobacteriaceae and

25 e isolates were observed to carry IncX4 type plasmid, among which 13 were identified to carry mcr-1 g

26 MLST and plasmid analysis shows that MCRPEC are diversely spread

27 c killing of the strain harboring the pIP501 plasmid and also proved to be cross-reactive against oth

28 ghly conserved parABS partitioning system in plasmid and chromosome segregation.

29 and 48.5 kbp dsDNA analytes, including both plasmid and genomic DNA.

30 phi in cell culture, and stably maintain the plasmid and GFPuv expression under antibiotic treatment

31 We further show that E. coli plasmid and PCR-derived DNA can efficiently transform C.

32 CRISPR arrays contain spacers homologous to plasmid and temperate phage sequences and, in some cases

33 tra- and interpatient diversification at the plasmid and transposon level was observed, significantly

34 We describe novel CRISPR/Cas9 transfection plasmids and approaches for the speedy, stable and marke

35 in cells transfected by pEF-GFP and pUB-GFP plasmids and for at least 5 days post-transfection for c

36 A fragments from invading bacteriophages and plasmids and integrate them as spacers into bacterial CR

37 Plasmids and other mobile elements are central contribut

38 enome binning, we show that our method links plasmids and other mobile genetic elements to their host

39 sites and thus facilitating their spread via plasmids and phages.

40 isms and approximately 600 novel viruses and plasmids and representing common experimental setups.

41 e transcript, reducing the size of mutagenic plasmids and simultaneously simplifying their design.

42 histidine relaxases among small promiscuous plasmids and their preference toward Gram-positive bacte

43 rionaceae chromosome arose from an ancestral plasmid, and that RctB may have evolved additional regul

44 The genes are located on two separate plasmids, and are probably paralogs or pseudoparalogs.

45 mplex of Shigella flexneri contains invasion plasmid antigen D (IpaD), which initially regulates secr

46 IncX4 plasmids are associated with the dissemination of the mc

47 The pPSU1 and pPSU2 plasmids are available without licensing restrictions to

48 Bacterial plasmids are extrachromosomal DNA that provides selectiv

49 global human health threat, and conjugative plasmids are important drivers of the rapid spread of re

50 As in other transposome systems, no helper plasmids are required since transposases are not express

51 ight against foreign DNA, such as phages and plasmids, as well as a revolutionary gene editing tool.

52 An invader plasmid assay showed that mutation either in the histidi

53 en, Campylobacter jejuni, can carry multiple plasmids associated with antibiotic resistance or virule

54 The plasmid-associated ColR genes, mcr-1 and mcr-2 were not

55 he ParA ATPase and specifically binds to the plasmid at a centromere-like site, parS.

56 tem and, in turn, conjugatively transfer the plasmid at high frequencies to recipient cells.

57 y co-transfecting AAV2, AAV8 and AAV9 helper plasmids at a ratio of 1:1:1.

58 tudy, we co-transfected AAV2 and AAV8 helper plasmids at different ratios (3:1, 1:1 and 1:3) to assem

59 However, an additional nick in the donor plasmid backbone markedly improved the gene-editing effi

60 udy aimed at determining general patterns of plasmid-bacteria evolution.

61 We characterized here a series of plasmid-based DSB templates that were repaired in Xenopu

62 Compared to conventional plasmid-based expression vectors and donor templates, we

63 bly corresponding to all classes of observed plasmid-based phenotypic resistance.

64 Using a plasmid-based reporter we found that LuxR can mediate re

65 coli UPEC-RIY-4, as well as their associated plasmid-borne antibiotic resistance genes (ARGs).

66 Biosynthesis of OXT-A has been linked to a plasmid-borne Bacillus megaterium gene cluster that cont

67 was revealed in Escherichia coli expressing plasmid-borne NMB0419 and showing significantly increase

68 n experiments, the DeltasfaA mutant carrying plasmid-borne sfaA restored the growth fitness in absces

69 fend against invading phages and conjugative plasmids by introducing site-specific double-stranded br

70 selfish genetic elements such as viruses and plasmids, by specific degradation of invader DNA or RNA.

71 Genetic complementation with a plasmid carrying the M.tb H37Rv sequence of eccCa1-eccCb

72 stid flagellate Parabodo caudatus with three plasmids carrying a fluorescence reporter gene (pEF-GFP

73 Plasmids carrying blaCMY-2 were assigned predominantly t

74 pendently of how they entered the cell, most plasmids clustered in the cytoplasm.

75 In vivo, transfection efficiency of a plasmid co-expressing hSef-b/eGFP into TRAMP C2 tumors w

76 lls from immune donors were transfected with plasmids coding for the most abundant proteins from the

77 ing Cas9/crRNA ribonuclear protein and donor plasmid coelectroporation, followed by fluorescence-base

78 utive promoter (2x CaMV 35S) was used in the plasmid construction, but seven transgenic lines were ob

79 Plasmid constructs containing fluorescent proteins or ta

80 igh rate of integration of the reprogramming plasmid containing a shRNA against TP53.

81 iferase activity in cells transfected with a plasmid containing FXI-3'UTR.These results should open t

82 strains possess an epidemic pCSZ4-like IncX4 plasmid containing mcr-1.

83 its medium and then uses them to replicate a plasmid containing the unnatural base pair dNaM-dTPT3.

84 Plasmids containing multiple copies of (G3T)n and (G3T4)

85 l sample with a high copy number of JCV or a plasmid control.

86 quantifying the cell-to-cell variability of plasmid copy number in bacteria.

87 A cycling in Escherichia coli in the form of plasmid copy number oscillations via a modular design th

88 software tools for extracting and assembling plasmid data from whole genome sequencing projects.

89 reducing construct size versus conventional plasmids) deployed with magnetofection achieve the highe

90 For high-copy-number plasmids, diffusion ensures that both daughter cells inh

91 tified Fbs1 variants through mutagenesis and plasmid display selection, which possess higher affinity

92 tated genes in the core genome); substantial plasmid diversity (>/=9 replicon types); and substantial

93 dermal administration of nucleic acids, both plasmid DNA (pDNA) and siRNA, to treat localised disease

94 Oral administration of the plasmid DNA (pDNA) encoding GLP-1 decreased diabetic glu

95 caused restriction of incoming bacteriophage/plasmid DNA and endogenous chromosomal DNA within Escher

96 Heterologous prime-boost immunization with plasmid DNA and viral vector vaccines is an emerging app

97 with spiky surfaces demonstrate the highest plasmid DNA binding capability and transfection efficacy

98 Sufficient plasmid DNA can be isolated from 100 ml E. coli cultures

99 We monitored plasmid DNA degradation using gel electrophoresis and qP

100 Importantly, use of plasmid DNA enabled lower depth sequencing, and assembli

101 We utilized two kinds of plasmid DNA encoding either BMP-2 or FGF-2 formulated in

102 , we administered intracranial injections of plasmid DNA encoding IL-10 (pDNA-IL-10) into the NAc of

103 e prepared via freeze drying and loaded with plasmid DNA encoding perlecan domain I and VEGF189 and a

104 The plasmid DNA encoding perlecan domain I and VEGF189 loade

105 s suggest that chitosan scaffolds containing plasmid DNA encoding VEGF189 and perlecan domain I have

106 uine IgG obtained from horses immunized with plasmid DNA followed by boosting with Kunjin replicon vi

107 ecifically evaluated the advantages of using plasmid DNA for sequencing and the value of supplementin

108 Circular carrier plasmid DNA is included during subsequent DNA purificati

109 improves microparticle-based transfection of plasmid DNA lipoplexes in several primary human cell typ

110 antly enhanced survival compared to the same plasmid DNA loaded in DNA-CN in two aggressive orthotopi

111 Plasmid DNA molecules with unique loop structures have w

112 Finally, DNA-BPN loaded with anti-cancer plasmid DNA provided significantly enhanced survival com

113 Plasmid DNA sequencing of previously uncharacterized cli

114 1 muL droplet of whole blood, and we amplify plasmid DNA spiked into whole blood droplets to represen

115 Furthermore, the efficient amplification of plasmid DNA spiked into whole blood proves that the larg

116 ionalized fluorescent probes, antibodies, or plasmid DNA to living cells requires overcoming the plas

117 Using HIV-lentivector and plasmid DNA transfection, we also developed A2A and A2B

118 Unlike T4CPs involved in plasmid DNA translocation, DotL appeared to function by

119 te nanotopography of silica nanoparticles as plasmid DNA vectors has significant impact on the transf

120 or CMV, or transient transfection with naked plasmid DNA, HIRA re-localizes to PML bodies, sites of c

121 is co-transfected with transposon-containing plasmid DNA, it penetrates prokaryotic or eukaryotic cel

122 e local microbubble-enhanced sonoporation of plasmid DNA.

123 h viral genomes, binds to incoming viral and plasmid DNAs and deposits histone H3.3 onto these.

124 ong double-stranded DNAs, including circular plasmid DNAs and genomic DNAs.

125 loads in the organs, demonstrating that the plasmid does not influence pathogenicity.

126 by twofold to fivefold relative to circular plasmid donors at one genomic locus in 293 T cells and t

127 which is comparable to therapy results with plasmid-driven L. lactis Initial blood glucose concentra

128 enesis method performed on routinely prepped plasmid dsDNA.

129 The genes encoding CS30 were located on a plasmid (E873p3) together with the genes encoding LT and

130 he insert between three high-copy expression plasmids enables the bulk purification of the aminoacyl-

131 The DNA plasmid encoded a MultiHIV B clade fusion protein design

132 progress on the pathogenic functions of the plasmid-encoded open reading frames, which may motivate

133 culation, a C. muridarum strain deficient in plasmid-encoded pGP3 or pGP4 but not pGP5, pGP7, or pGP8

134 mechanism that requires a multi-functional, plasmid-encoded replication protein to initiate replicat

135 Previously, we exploited the plasmid-encoded toxin (Pet) autotransporter system for a

136 e plasmid of a transposon from a co-residing plasmid encoding a putative toxin-antitoxin system; iii)

137 These plasmids were combined with a plasmid encoding cytokine IL-28B, together named as VGX-

138 Most Chlamydia species carry a 7.5kb plasmid encoding eight open reading frames conventionall

139 te in mice by hydrodynamic transfection of a plasmid encoding for tissue-type plasminogen activator (

140 esveratrol (RES) and then transfected with a plasmid encoding HIV1-gp120.

141 e were given intraperitoneal injections of a plasmid encoding LNA-anti-uc.173, to knock down endogeno

142 Here, we successfully delivered a plasmid encoding S. pyogenes Cas9 and sgRNA to the corne

143 eduction in transformation efficiency of the plasmid encoding the self-targeting crRNA.

144 icity on replication, we engineered proviral plasmids encoding diverse RTs within the backbone of HIV

145 lar genetic contexts were found in different plasmids, even the E. coli chromosome, implying the acqu

146 Here, we show that common conjugal plasmids, even when costly, are indeed transferred at su

147 enomics showed that the mcr-1-carrying IncX4 plasmids exhibit remarkable similarity in the backbone,

148 patient was corrected by delivering a CRISPR plasmid expressing Cas9/gRNA and a single-stranded oligo

149 h a Zaire ebolavirus glycoprotein expression plasmid followed by infection with a vesicular stomatiti

150 we found that a large conjugative resistance plasmid follows the same evolutionary trajectories as it

151 ave engineered specific fluorescent reporter plasmids for quantitative measurements of 8-oxoguanine D

152 corporates DNA fragments (spacers) from both plasmid (foreign) and host genome (self) sequences into

153 Together these plasmids form a toolkit that will allow the rapid genera

154 Plasmid-free C. trachomatis is also attenuated in both t

155 e in vivo but not in vitro phenotypes of the plasmid-free organisms, suggesting that pGP3 is a key in

156 as protein over two orders of magnitude in a plasmid-free system.

157 TPA is capable of assembling a 7 kb plasmid from 10 fragments at approximately 80% fidelity

158 ts at approximately 80% fidelity and a 31 kb plasmid from five fragments at approximately 50% fidelit

159 east Saccharomyces cerevisiae, on a low-copy plasmid from two identical P TDH3 promoters containing a

160 The notion is applicable to nine plasmids from six major incompatibility groups and mixed

161 validated the results observed with the DNA plasmid, further supporting that PPTSP44 constitutes a p

162 s and the gammaherpesviruses, maintain their plasmid genomes by tethering them to cellular chromosome

163 ht open reading frames conventionally called plasmid glycoproteins 1-8 or pGP1-8.

164 Although plasmids harbor biomedically important genes, (such as g

165 n (rib) operon and presence of four putative plasmids harbouring rib operons.

166 Similar virulence plasmids have been acquired by other B. cereus strains a

167 own to increase plasmid persistence in other plasmid-host pairs, our work points towards common mecha

168 P8) in a high-expression plasmid or in a CEN plasmid in the presence of recessive mutations in genes

169 as located on the Variable Region I of IncX4 plasmids in 11 E. coli isolates.

170 Resequencing of three plasmids in a reference Klebsiella pneumoniae isolate de

171 lular localization of sRNAs transcribed from plasmids in Escherichia coli using RNA fluorescent in-si

172 al clustering with regional spread of IncHI2 plasmids in Europe and IncI2 in Asia.

173 detection of the GM strain and its putative plasmids in food and feed products have been developed.

174 s and the distribution patterns of virulence plasmids in isolates from nurseries.

175 tant multidrug-resistant clones and epidemic plasmids, in American crows.

176 Y-2 were assigned predominantly to IncA/C (8 plasmids), IncI1/ST23 (5) and IncI1/ST12 (3).

177 Amongst a total of 13 plasmid incompatibility types, the IncI2, IncX4 and IncH

178 ne transfer dominated by a limited number of plasmid incompatibility types.

179 systems provide protection against viral and plasmid infection by capturing short DNA sequences from

180 We generated a two-plasmid infectious clone system from which infectious vi

181 This BioBrick plasmid insert encodes 30 of the 31 translation factors

182 The findings suggest that ancestral plasmid instability can at least partly be explained by

183 mRNAs by transfection of Luciferase reporter plasmids into Huh7, BNL-1ME, and HEK293 cultured cell li

184 ation efficiencies of modified or unmodified plasmids into isogenic S.

185 The cryptic plasmid is essential for Chlamydia muridarum disseminati

186 Although the plasmid is not critical for chlamydial growth in vitro,

187 wth, and that the acquisition of a virulence plasmid is sufficient to transition beneficial symbionts

188 The acquisition of a virulence plasmid is sufficient to turn a beneficial strain of Rho

189 from a library of transfected, promoter-less plasmids is recombined into the landing pad in each cell

190 Gene expression in the IncP-1 plasmids is stringently controlled by a network of four

191 peg-344 is present on the hvKP1 virulence plasmid, is broadly prevalent among hvKP strains, and ha

192 In SuRE, a plasmid library of random genomic fragments upstream of

193 mbining conjugation inhibition and promoting plasmid loss would be an effective strategy to limit con

194 higher-order complex formation and long-term plasmid maintenance.

195 equencing revealed that the strain carried a plasmid-mediated CTX-M-15 ESBL gene and did not belong t

196 Mutational and plasmid-mediated mechanisms of colistin resistance have

197 faeces were tested for Escherichia coli with plasmid-mediated quinolone resistance (PMQR), extended-s

198 mase-producing type SHV-12), and quinolones (plasmid-mediated quinolone resistance gene qnrB7).

199 Recently, the mcr-1 gene was identified as a plasmid-mediated resistance mechanism in human and anima

200 Plasmid-mediated synthesis of eIF4G imposes increased gl

201 rough small interfering RNA knockdown or MCU plasmid-mediated up-regulation, as well as through modul

202 echnique capable of delivery of multiple DNA plasmids, messenger RNAs, and recombinant proteins is de

203 ulates the full range of actively segregated plasmid motilities observed in vivo.

204 tion are the most commonly observed modes of plasmid motility in vivo, we suggest that the operation

205 We demonstrate that plasmid motility is tuned as the replenishment rate of t

206 In contrast, most low-copy-number plasmids need to be actively partitioned by a conserved

207 rfA1); ii) the acquisition by the resistance plasmid of a transposon from a co-residing plasmid encod

208 allotopic ATP8 (nATP8) in a high-expression plasmid or in a CEN plasmid in the presence of recessive

209 We identified >3000 NB genes located on plasmids or on the chromosome from 53 bacterial species

210 nsfected fusion and hemagglutinin expression plasmids or with syncytium-based assays in Vero, Vero-SL

211 ng exopolyphosphatase PPX from a recombinant plasmid, or by the introduction of deletion alleles in p

212 Yet, replication from an R-loop-initiating plasmid origin kills the double rnhAB mutant, revealing

213 Furthermore, within a single host-plasmid pair three distinct patterns of adaptive evoluti

214 y for acquiring and disseminating resistance plasmids, particularly variants of the K. pneumoniae car

215 rected segregation maximizes the fidelity of plasmid partition.

216 Host factors involved in plasmid partitioning can be functionally separated by th

217 Plasmid partitioning can be remarkably robust.

218 reafter) device was used to deliver reporter plasmids (pCMVbeta and pEGFP-N1) into viable excised hum

219 A recombinant Asd(+) plasmid pCZ1 with the cloned Salmonella Choleraesuis O-a

220 ons individually have been shown to increase plasmid persistence in other plasmid-host pairs, our wor

221 terns of adaptive evolution led to increased plasmid persistence: i) mutations in the replication pro

222 ds to assemble cyclic sequences likely to be plasmids, phages and other circular elements.

223 roduction system, we used an HBoV1 packaging plasmid, pHBoV1NSCap, that harbors HBoV1 nonstructural p

224 uated the protein TraM, from the conjugative plasmid pIP501, as a potential vaccine candidate.

225 ed with Klebsiella pneumoniae, a predominant plasmid (pKpQIL), and a specific transposable element (T

226 the MOBV relaxase MobM from the promiscuous plasmid pMV158 in complex with several origin of transfe

227 rovide validation by DNA amplification of 77 plasmids predicted by Recycler from the different sequen

228 as found on high copy number, small Col-like plasmids, previously associated with horizontal transmis

229 As such, the pPSU1 and pPSU2 plasmids provide reference fragments from 50 to 10000 bp

230 The clustering of KSHV plasmids provides it with an effective evolutionary stra

231 We treated plasmid pWH1266, which contains ampicillin resistance ge

232 rcle origins of replication, and areas where plasmids recombined with the host chromosome.

233 ecycler greatly increases the number of true plasmids recovered relative to other approaches while re

234 were preferentially acquired from genome or plasmid regions corresponding to active transposons, CRI

235 However, a plasmid relaxation test failed to detect R-tracts in DNA

236 s, scarless multi-fragment assembly of large plasmids remains challenging.

237 When the plasmid replicates, the daughters largely display motili

238 entral two domains resembles that of several plasmid replication initiators.

239 host cell reactivation assay, we show that a plasmid reporter bearing a site-specific 3,N4-ethenocyto

240 Parallel RNA-Seq expression analysis of the plasmid reporter identifies novel transcriptional mutage

241 The plasmids required approximately 20-25 mJ/cm(2) per log10

242 against turnaround time, full annotation of plasmid resistance gene content could be obtained in und

243 ion, along with the distribution patterns of plasmids, reveals the impact of horizontal gene transfer

244 h Linear Array (LA) genotyping results for 9 plasmid samples and fully or partially concordant for 9

245 ctates the tempo of ParF assembly cycles and plasmid segregation events by stimulating ParF adenosine

246 een shaped by evolution for high fidelity of plasmid segregation.

247 cessary, are not sufficient per se to ensure plasmid segregation.

248 We performed genomic and plasmid sequencing of three clinical isolates with both

249 Repeated TUS treatments with hSef-b plasmid, significantly suppressed prostate tumor growth

250 t of segregation; the symmetric tethering of plasmid sisters to sister chromatids embodies the replic

251 of single nicks in the target gene and donor plasmid (SNGD) using Cas9D10A nickase promotes efficient

252 of inter-species, regional and international plasmid spread.

253 our work points towards common mechanisms of plasmid stabilization.

254 Compared to the multi-plasmid system, this all-in-one vector activates gene ex

255 lopment of medical utility of the chlamydial plasmid system.

256 DNA plasmids targeting the HCV non-structural antigens NS3,

257 ansformation of R. typhi with the pRAM18dRGA plasmid that originally derives from Rickettsia amblyomm

258 previously thought, in terms of the range of plasmids that can be transferred by conjugation and the

259 quences derived from prokaryotic viruses and plasmids that determine the targets of the host's CRISPR

260 account for the length of the entire pWH1266 plasmid, the qPCR rate constants were 2-7x larger than t

261 y pGP4 and pGP5, respectively, may allow the plasmid to promote chlamydial adaptation to varied anima

262 hhiking model, random tethering of a pair of plasmids to chromosomes signifies the replication-indepe

263 We also generated expression plasmids to ectopically express all 7 chitinases in our

264 ted from 100 ml E. coli cultures for the two plasmids to produce 100 bp or 1 kb ladders for 1000 gels

265 shown to select for adaptation of resistance plasmids to their new bacterial hosts, or vice versa, a

266 Experiments with single plasmid topoisomers showed that the average cooperativit

267 Whereas ParF specifies the territory for plasmid trafficking, the ParG partner protein dictates t

268 s of irradiated, Hey2 siRNA- and Hey2 vector plasmid-transfected human umbilical vein endothelial cel

269 e context of segment 7 RNPs reconstituted by plasmid transfection and in mutant virus-infected cells.

270 of BE3 confers higher specificity even than plasmid transfection of HF-BE3, while maintaining compar

271 Plasmid transfection was used to modulate transforming g

272 verexpression of hsp60, achieved by in vitro plasmid transfection, was then performed to determine wh

273 as knocked down by siRNA or overexpressed by plasmid transfection.

274 or many gram-positive pathogens, conjugative plasmid transfer is an important means of spreading anti

275 ntisense sRNA and the traJ mRNA to control F plasmid transfer.

276 at high frequencies (10(-1) to 10(-3)), and plasmid types and MCRPEC multi-locus sequence types (MLS

277 ins were analysed for antibiotic resistance, plasmid typing, and transfer analysis, and strain relate

278 systems have recently been documented using plasmid, viral, or fragmented genomic DNA; dPCR performa

279 nd the VRC 320, done in one centre, assessed plasmid VRC5283 (wild-type Zika virus).

280 C 319 trial, done in three centres, assessed plasmid VRC5288 (Zika virus and Japanese encephalitis vi

281 Well-studied plasmid Walker-box partition modules require ParA, centr

282 alysis of this revealed that the integration plasmid was frequently lost and that apidaecin expressio

283 in which the rate of loss of the integration plasmid was much lower after induction, and which produc

284 The predominant blaKPC-positive plasmid was pHS102707 (n = 62, 55.4%) and the predominan

285 , 55.4%) and the predominant blaNDM-positive plasmid was pNDM-ECS01 (n = 46, 48.9%).

286 We previously showed that a broad-host-range plasmid was poorly maintained in Shewanella oneidensis,

287 which a reduced dG-AP cross-link-containing plasmid was replicated in cultured human cells.

288 signaling in the cells transfected with nsp5 plasmid was significantly inhibited.

289 These plasmids were combined with a plasmid encoding cytokine

290 The major variable regions of all the IncX4 plasmids were fully characterized by PCR-RFLP.

291 Plasmids were generated carrying reporter genes for fluo

292 Three representative mcr-1-positive IncX4 plasmids were selected for high-throughput sequencing.

293 PSU1 & pPSU2 pair of molecular weight marker plasmids which produce both 100 bp and 1 kb DNA ladders

294 that were transfected with the pERRE3tk-LUC plasmid, which demonstrated transactivation of GOT.

295 ngineered lentivirus-driven miRNA expression plasmids, which we tested in the mouse placenta.

296 We used recircularization of a linearized plasmid with 3-P-blocked termini, mimicking those at X-r

297 To test this hypothesis, we injected plasmids with sequence variations flanking an I-SceI end

298 We also found many plasmids within the chromosomal volume, providing furthe

299 se chain reaction-amplified fragments into a plasmid without the use of enzymes.

300 r large (1.1 Mb) synthetic yeast centromeric plasmids (YCps) to cultured cell lines at rates similar