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1 d ssDNA and stabilize negative supercoils in plasmid DNA.
2 immune responses following immunization with plasmid DNA.
3 key phosphorylation site involved in DNA-PK) plasmid DNA.
4 a novel eukaryotic alternative to bacterial plasmid DNA.
5 mited by detection of contaminating viral or plasmid DNA.
6 rocapsule position on cell transfection with plasmid DNA.
7 plexes formed using BFDMA and macromolecular plasmid DNA.
8 crA helicase to bind and begin unwinding the plasmid DNA.
9 ed muscles over muscles treated with "naked" plasmid DNA.
10 nse than intramuscular injection of the same plasmid DNA.
11 and DNA nodes, within negatively supercoiled plasmid DNA.
12 in vivo and in human genomic DNA spiked with plasmid DNA.
13 iates at multiple single-stranded regions of plasmid DNA.
14 DNA preparations are contaminated with XMRV plasmid DNA.
15 cles (VLPs) when expressed individually from plasmid DNA.
16 tion of 12 novel DeltaG rabies variants from plasmid DNA.
17 actions participate in the immunogenicity of plasmid DNA.
18 oduction of a single negative supercoil into plasmid DNA.
19 he promoter region of the human BCL2 gene in plasmid DNA.
20 ed only in HEK293 cells transfected with the plasmid DNA.
21 e local microbubble-enhanced sonoporation of plasmid DNA.
22 n health, often residing in extrachromosomal plasmid DNA.
23 ved 20% 5-gmC in the genome and 45% 5-gmC in plasmid DNA.
24 wer for the attenuated DNA and close for the plasmid DNA.
25 asmid DNA and per 10(8) cells with a 13.8 kb plasmid DNA.
26 m minicircles was 5-10-fold higher than with plasmid DNA.
27 st defense by interfering with the uptake of plasmid DNA.
28 (HFFs) infected with HSV or transfected with plasmid DNA.
29 and influencing intracellular trafficking of plasmid DNA.
30 264.7 and DC 2.4 cells using GFP-expressing plasmid DNA.
31 omplexes with a high packaging efficiency of plasmid DNA.
32 idge between PEGylated anionic liposomes and plasmid DNA.
34 issue, we used intradermal immunization with plasmid DNA, a system in which activation of CD8(+) T ce
36 und that mice injected with 50mug luciferase plasmid DNA and 5x10(5) microbubbles followed by ultraso
39 caused restriction of incoming bacteriophage/plasmid DNA and endogenous chromosomal DNA within Escher
40 cationic polymers and fluorescently labeled plasmid DNA and injected them intradermally into mice.
41 to consistently enhance the delivery of both plasmid DNA and messenger RNA payloads in stem cells, pr
42 formation efficiency up to 10(7) CFU per mug plasmid DNA and per 10(8) cells with a 13.8 kb plasmid D
43 1(+) rhesus monkeys through vaccination with plasmid DNA and recombinant adenovirus encoding simian-h
44 onfocal live cell imaging, we show that both plasmid DNA and siRNA are internalised via endocytosis.
46 homatis mouse pneumonitis (MoPn) genomic and plasmid DNA and tested it with serum samples from MoPn-i
47 Heterologous prime-boost immunization with plasmid DNA and viral vector vaccines is an emerging app
51 , were susceptible to the virus rescued from plasmid DNAs and supported production of the virus over
52 es were evaluated using combinations of both plasmid-DNA and transposase-protein relocalization to th
54 s, germline transgenesis, electroporation of plasmid DNA, and microinjection of morpholinos are all r
55 es not affect the biological activity of the plasmid DNA, and that the core-shell formulations have n
56 is not required for efficient replication of plasmid DNA, and they suggest that DNA functions as a co
58 of redox control observed for lipoplexes of plasmid DNA are maintained in complexes formed using sma
59 including small interfering RNA (siRNA) and plasmid DNA, are important tools for the study of mammal
62 now prime DNA synthesis using the recipient plasmid DNA as template, circumventing a lesion that blo
63 , we tested a different formulation in which plasmid DNA associates with the surface of preformed 20-
64 human and canine cells partition transfected plasmid DNA asymmetrically, preferentially into the daug
66 necessary to orchestrate the propagation of plasmid DNA between bacterial cells through conjugative
67 with spiky surfaces demonstrate the highest plasmid DNA binding capability and transfection efficacy
68 , BALB/c mice were given three injections of plasmid DNA-Bla g 1 prior to sensitization with two prim
71 h cisplatin enhances double-strand breaks of plasmid DNA by a factor of approximately 3.5 and dramati
72 rt that (-)-lomaiviticin A nicks and cleaves plasmid DNA by a pathway that is independent of reactive
73 nwinding of both linear and natural circular plasmid DNA by PcrA/RepD was followed in real-time using
75 he type IV REase in restricting 5mC-modified plasmid DNA by transformation into clinical S. aureus st
78 le-mediated transcutaneous immunization with plasmid DNA can potentially induce a stronger immune res
79 e screening, rolling circle amplification of plasmid DNA, capillary electrophoresis and automated dig
80 eractive transposase, together with circular plasmid DNA carrying a transgene construct flanked by bi
81 SB100X hyperactive transposase together with plasmid DNA carrying a transgene construct flanked by bi
82 Stable transfection of HEK-293T cells with plasmid DNA carrying EqCXCL16 (HEK-EqCXCL16 cells) incre
83 with saline, injected with uncompacted naked plasmid DNA carrying the Rds gene, or remained untreated
84 measurements of a model system of clustered plasmid-DNA centered by the dynamically unstable actin-l
86 licited a stronger immune response than with plasmid DNA-coated net negatively charged nanoparticles
89 rticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-ly
90 noparticles, composed of single molecules of plasmid DNA compacted with block copolymers of poly-l-ly
91 linked to itself interacts differently with plasmid DNA compared to conventional vectors and when te
92 hese data demonstrate that mucosally applied plasmid DNA complexed to PEI followed by a mucosal prote
93 Significant differences in behavior toward plasmid DNA condensation are correlated with biological
94 MS11 was enhanced by the addition of excess plasmid DNA containing a DUS while FA1090 transformation
95 ional non-viral gene transfer uses bacterial plasmid DNA containing antibiotic resistance genes, cis-
96 say using mammalian cell nuclear extract and plasmid DNA containing bulky adducts formed by N-acetoxy
98 thin the procapsid and recircularizes linear plasmid DNA containing two terminal loxP recognition sit
100 was shown to be required for replication of plasmid DNAs containing covalent DNA-protein complexes.
101 cted nucleosome positioning in vitro on four plasmid DNAs containing DNA fragments derived from the g
103 ceptor-targeted, DNA-binding peptide (P) and plasmid DNA (D), which electrostatically self-assembled
107 iption-coupled hypernegative supercoiling of plasmid DNA did not need the expression of a membrane-in
110 strate co-delivery of luciferase protein and plasmid DNA encoding a fluorescent protein from two diff
112 ing a natural phenomenon observed in tumors, plasmid DNA encoding for a soluble ligand to NKG2D (sRAE
114 , we administered intracranial injections of plasmid DNA encoding IL-10 (pDNA-IL-10) into the NAc of
115 e prepared via freeze drying and loaded with plasmid DNA encoding perlecan domain I and VEGF189 and a
117 umor immunity induced upon immunization with plasmid DNA encoding SV40 Tag as a transgene (pCMV-Tag).
120 we inoculated nonhuman primates (NHPs) with plasmid DNA encoding transmembrane-anchored, cleaved JRF
121 s suggest that chitosan scaffolds containing plasmid DNA encoding VEGF189 and perlecan domain I have
125 UvrC from Mtb collectively bound and cleaved plasmid DNA exposed to ultraviolet (UV) irradiation or p
126 d hydrodynamic injection to deliver a CRISPR plasmid DNA expressing Cas9 and single guide RNAs (sgRNA
127 uine IgG obtained from horses immunized with plasmid DNA followed by boosting with Kunjin replicon vi
128 ecifically evaluated the advantages of using plasmid DNA for sequencing and the value of supplementin
129 can be genetically programmed with nonviral plasmid DNA for the biogenesis and delivery of antisense
130 ation of parasites in a sample, we developed plasmid DNAs for all the three assay targets for absolut
131 We show that peptide efficiently packaged plasmid DNA forming spherical, highly cationic nanocompl
133 i, such as by insertion of foreign (viral or plasmid) DNA fragments into clustered regularly interspa
135 ve B. burgdorferi much more efficiently than plasmid DNA from E. coli, particularly when the bbe02 an
137 rBCP was also found to protect supercoiled plasmid DNA from oxidative damage (i.e., nicking) in vit
138 or CMV, or transient transfection with naked plasmid DNA, HIRA re-localizes to PML bodies, sites of c
139 sp and Imu1-3 and examined their activity on plasmid DNA, human umbilical vein endothelial cells, and
140 distinct types of T cell immunity, improves plasmid DNA immunization, including mobilization of CD8(
141 , spontaneous brain tumors were induced with plasmid DNA in a matter of weeks in three separate mouse
142 persistent transgene expression compared to plasmid DNA in a number of organ systems but has not bee
143 cytic choriomeningitis virus infection using plasmid DNA in a prime, but not prime-boost, vaccination
144 study demonstrates for the first time use of plasmid DNA in absolute quantification of malaria parasi
145 Polyphenols extracted from honeys degraded plasmid DNA in the presence of H2O2 and Cu(II) in the Fe
146 on of HPV-11, -16, and -18 origin-containing plasmid DNA in transfected cells at concentrations well
147 n this study we compared minicircle DNA with plasmid DNA in transfections of airway epithelial cells.
148 transfection of luciferase and GFP reporter plasmid DNA in vitro and in vivo under various condition
150 reconstituted pre-RCs support replication of plasmid DNA in yeast cell extracts in a reaction that ex
152 mbinase (ZFR) fusion proteins that integrate plasmid DNA into a synthetic target site in the human ge
153 ng protein that targets chromosomes, carries plasmid DNA into cells, and shows specificity for active
156 used to directly catalyze the integration of plasmid DNA into mammalian genomes, there is still an un
157 The cationic Man-CS-Phe micelles condense plasmid DNA into nanoscale polyplexes and provide effici
159 ped two polymeric gene carriers that compact plasmid DNA into small and highly stable nanoparticles w
160 optimized protocol for biolistic delivery of plasmid DNA into the epidermis of Arabidopsis leaves, wh
161 IFI16 also restricted the expression of plasmid DNAs introduced by transfection but did not rest
162 wever, whether the immunogenic properties of plasmid DNA involve TLR9 signaling has not been clearly
163 n-induced single-strand-break (SSB) yield in plasmid DNA involves measurement of the fraction of rela
168 orbent surface leads to full recovery of all plasmid DNA isoforms, which is a major issue when using
169 d, with the best-performing model applied to plasmid DNA isolated from Escherichia coli and mitochond
170 is co-transfected with transposon-containing plasmid DNA, it penetrates prokaryotic or eukaryotic cel
171 improves microparticle-based transfection of plasmid DNA lipoplexes in several primary human cell typ
172 antly enhanced survival compared to the same plasmid DNA loaded in DNA-CN in two aggressive orthotopi
176 e prophylactic and therapeutic efficacy of a plasmid DNA-mediated vaccination using the Bla g 1 gene
177 ptide with a nucleic acid binding domain and plasmid-DNA, minicircle-DNA or small interfering RNA (si
180 ata demonstrated that the mean SSB yield per plasmid DNA molecule of [21.2+/-0.59]x10(-2)Gy(-1) as me
181 oscopy confirmed that mouse PPy/u containing-plasmid DNA molecules under the different structural str
183 -DNA nanoparticles are capable of delivering plasmid DNA of different size into cells in culture, yie
184 imultaneous delivery of siRNA and linearized plasmid DNA on the surface of a single nanocrystal provi
185 s were significantly enhanced by coating the plasmid DNA on the surface of cationic nanoparticles.
186 d by transcutaneous immunization by applying plasmid DNA onto a skin area pretreated with solid micro
187 that transcutaneous immunization by applying plasmid DNA onto a skin area wherein the hair follicles
189 ys, the transfected cell microarray, wherein plasmid DNA or siRNA, spotted on the surface of a substr
190 o this include making use of vectors such as plasmid DNA or viruses, live attenuated pathogens or sub
191 ved ORF analyses showed that the majority of plasmid DNAs originated within red algae, whereas others
194 dermal administration of nucleic acids, both plasmid DNA (pDNA) and siRNA, to treat localised disease
195 ic polyamine disulfides to condense and cage plasmid DNA (pDNA) by a process of thermodynamically con
196 on of macrophages transfected ex vivo with a plasmid DNA (pDNA) encoding a potent antioxidant enzyme,
202 HIV-derived conserved element (CE) p24(gag) plasmid DNA (pDNA) vaccine is able to redirect immunodom
203 ase or angiotensin II type 2 receptor (AT2R) plasmid DNA (pDNA) was evaluated in Lewis lung carcinoma
204 dle delivery of nucleic acids, in particular plasmid DNA (pDNA), to the skin represents a potential n
205 in which the liposomes are designed to carry plasmid DNA (pDNA, containing luciferase reporter gene)
207 subcutaneous injection of cationized gelatin/plasmid DNA polyplex into the rat hindpaw and its subseq
208 ame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minic
211 e the transfection efficiency of GFP-encoded plasmid DNA (pRmHa3-GFP) into cells through efficient DN
214 Finally, DNA-BPN loaded with anti-cancer plasmid DNA provided significantly enhanced survival com
218 s more potent than deglycoBLM in supercoiled plasmid DNA relaxation, while the analogue having the di
219 imilar are not equivalent and that repair of plasmid DNA requires additional factor(s) that are not r
222 ry system can be extended to the delivery of plasmid DNA, siRNA, or aptamers for preclinical and clin
223 1 muL droplet of whole blood, and we amplify plasmid DNA spiked into whole blood droplets to represen
224 Furthermore, the efficient amplification of plasmid DNA spiked into whole blood proves that the larg
226 A recent publication showed that circular plasmid DNA standards grossly overestimated numbers of a
227 ed LDL-cholesterol oxidation and supercoiled plasmid DNA strand breakage inhibition induced by both p
229 as both MeC and C nucleobases in transfected plasmid DNA substrates are highly susceptible to editing
230 ) by combining traits reported to facilitate plasmid DNA synthesis (i.e., decreased Pyk flux and incr
234 n the successful construction of a series of plasmid DNA templates that contain many tandem copies of
236 a procedure for the isolation of total rumen plasmid DNA, termed rumen plasmidome, and subjected it t
237 Here we describe an iDNA vaccine composed of plasmid DNA that encode the full-length infectious genom
238 orms stable complexes at both ends of linear plasmid DNA that protect the DSBs from digestion by 5'-
239 teria and archaea from invasion by phage and plasmid DNA through a genetic interference pathway.
240 demonstrated superior condensing ability for plasmid DNA through the formation of compact nanosized p
241 al and liposomal vectors for the delivery of plasmid DNA to cells for gene therapy applications.
243 nanocarrier is found to effectively condense plasmid DNA to form a highly stable GNR-DSPEI-PEG-RGD/DN
244 e present study, we evaluated the ability of plasmid DNA to induce local and systemic antigen-specifi
245 ionalized fluorescent probes, antibodies, or plasmid DNA to living cells requires overcoming the plas
246 (DAB) dendrimer would allow the transport of plasmid DNA to the brain after intravenous administratio
247 imine dendrimer would allow the transport of plasmid DNA to the brain after intravenous administratio
250 f PEI can be effective delivery vehicles for plasmid DNAs to elicit cellular and humoral protective r
251 a strategy to study platinum cross-links on plasmid DNAs transfected into live mammalian cells based
252 ut, contributing to a 2.5~3 fold increase on plasmid DNA transfection and an additional 10-55% transg
253 initiation factor eIF-2alpha seen following plasmid DNA transfection were both greatly reduced in AD
255 s for gene therapy of airway disorders where plasmid DNA transfections have so far proven inefficient
260 ase of the bacterium (OD 6.0) using 25 ng of plasmid DNA under 100 Ohms resistance and 1.7 kV/cm volt
261 ounds exhibited significant photocleavage of plasmid DNA upon visible light irradiation and are rapid
262 ) and microRNA-125b-2 (miR-125b2) expressing plasmid DNA using hyaluronic acid-poly(ethylene imine)/h
263 1 chemokine MCP-1 during the early phases of plasmid DNA vaccination because injecting the type II NK
265 ecause cytotoxic T-cell responses induced by plasmid DNA vaccination were reduced in Sting-deficient
266 activation, during the first days following plasmid DNA vaccination, NKT cells release IL-5 and MCP-
269 an influenza challenge model we show that a plasmid DNA vaccine complexed to a less toxic form of PE
270 vatives of PEI could be utilised for topical plasmid DNA vaccine delivery to human mucosal tissue sur
273 IV-1 CN54gp140 antigen when cation-complexed plasmid DNA vaccines are applied topically to the murine
277 te nanotopography of silica nanoparticles as plasmid DNA vectors has significant impact on the transf
278 , a new method of introducing mutations into plasmid DNA via a light-mediated mutagenesis protocol wa
279 Efficient in vitro electrotransfection of plasmid DNA was demonstrated in several hard-to-transfec
283 led to a luciferase bioluminescence reporter plasmid DNA were shown to transfect tumors implanted in
284 and the mobilities of circular versus linear plasmid DNAs were also affected by the chemical form and
285 are incorporated into cells or organisms as plasmid DNA, which leads the transcriptional and transla
286 sition of horizontally transferred phage and plasmid DNA, which provides virulence and resistance fun
287 an be derived from biologically synthesized (plasmid) DNA, which reduces errors associated with chemi
288 rR links the filaments with centromeric parC plasmid DNA, while facilitating the addition of subunits
289 (TopA67) but not full-TopA in the absence of plasmid DNA, while PrS-YjhX binds to full-TopA in the pr
290 orphologies are prepared via condensation of plasmid DNA with a block copolymer of polyethylene glyco
293 ting stem-loop was confirmed by digestion of plasmid DNA with apurinic endonuclease IV, followed by p
297 nd simple system for delivering oncolytic Ad plasmid DNA with the bioreducible polymers, skipping tim
298 developed a novel system using oncolytic Ad plasmid DNA with two bioreducible polymers: arginine-gra
300 null AdnAB is a helicase that unwinds linear plasmid DNA without degrading the displaced single stran
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