ライフサイエンスコーパス: plasmid construct

1 in cells transfected with a GKLF-expressing plasmid construct.

2 ular mass of 33-34 kDa, as expected from the plasmid construct.

3 ortened form of the tyrR gene via a specific plasmid construct.

4 flexibility not available in genomic DNA or plasmid constructs.

5 various pharmacologic enzyme inhibitors and plasmid constructs.

6 ted with the wt MDR1 promoter DNA/pGL3-basic plasmid constructs.

7 ouse myeloma with the VH gene DNA in various plasmid constructs.

8 ietic progenitor cells compared with smaller plasmid constructs.

9 c myocytes were transiently transfected with plasmid constructs.

10 s lack integration potency compared to wtAAV plasmid constructs (10- to 100-fold differences) was une

11 ine kinase promoter-luciferase reporter gene plasmid construct and determining reporter gene expressi

12 Luciferase-expressing plasmid constructs and immunoblotting confirmed several

13 y employed transfection of p53 or HIF-1alpha plasmid constructs and/or p53 and HIF-1alpha reporter co

14 , transfected with small interfering RNA and plasmid constructs, and analyzed by immunocytochemistry.

15 l dipeptidyl-peptidase-like protein-6 (DPPX) plasmid constructs, and comparative brain immunostaining

16 rp3 and hrp2 sequences that were used in the plasmid constructs as gene control regions (chromosomes

17 This work uses whole plasmid constructs as well as oligonucleotide-based mimi

18 In THP-1 cells transfected with HIV plasmid constructs, both organisms induced transcription

19 estabilization can be dramatic; certain parS-plasmid constructs cannot even be maintained in the pres

20 Most of the Red plasmid constructs catalyzed efficient recombination in

21 the likelihood of in vivo instability of the plasmid constructs caused by constitutive hyperexpressio

22 have hydrodynamically transfected mice with plasmid constructs composed of a murine hepcidin 1 promo

23 ransformation of M. smegmatis with inhA on a plasmid construct conferred high-level resistance to INH

24 In transient transfection assays, a plasmid construct containing 1.3 kilobase pairs (kb) of

25 fragment contained a functional promoter, a plasmid construct containing 2433 base pairs of the gp13

26 MCF-7 and MCF-7/Adr cells with a pGL3-Basic plasmid construct containing a CAAT-deleted MDR1 promote

27 With a plasmid construct containing a promoterless cpe-gusA gen

28 The plasmid construct containing htpGp-->stxB1,eaeA was unst

29 sus octamer sequence in the TOP2 5' UTR in a plasmid construct containing only a single consensus oct

30 ated transfection of EC was performed with a plasmid construct containing the gene encoding eNOS.

31 The transcription of a plasmid construct containing the human M-CPT I gene prom

32 effect mediated by topical application of a plasmid construct containing the murine IFN-alpha 1 tran

33 for 8 weeks, followed by immunization with a plasmid construct containing the pre-S2/S gene that enco

34 A plasmid construct containing three repeat OREs and a het

35 08-15 cells was altered by transfection with plasmid constructs containing a full length cDNA of huma

36 ncrease in luciferase activity compared with plasmid constructs containing CAAT-deleted, GC-box, and

37 Plasmid constructs containing fluorescent proteins or ta

38 We designed a series of plasmid constructs containing fragments of regions 1 and

39 In the present study, plasmid constructs containing green fluorescent protein

40 UVECS were then transiently transfected with plasmid constructs containing ICAM-1 and HCMV immediate

41 RNAs were characterized by the use of NK-1R plasmid constructs containing intron sequences.

42 transfection experiments were conducted with plasmid constructs containing serial deletions of the ap

43 tional analysis by transient transfection of plasmid constructs containing site-specific mutations in

44 NFKB1 promoter/exon 1 luciferase reporter plasmid constructs containing the -94delATTG allele and

45 both DeltaNotI replicons and RUB replicon or plasmid constructs containing the C gene resulted in rep

46 ndent transcriptional activation of reporter plasmid constructs containing the consensus element.

47 transfections of A549 cells with pGL3-Basic plasmid constructs containing the functional hTERT promo

48 enotype of these mutants was complemented by plasmid constructs containing the wild-type o359 allele,

49 Plasmid constructs containing these sequences were repli

50 Plasmid constructs containing up to 1600 base pairs upst

51 ed with QR-chloramphenicol acetyltransferase plasmid constructs containing various portions of the 5'

52 sitions of PE I and II sequences in reporter plasmid constructs (containing chloramphenicol acetyltra

53 The data on expression of DT from the plasmid constructs demonstrate that the basal level of D

54 ctivation and dominant positive and negative plasmid constructs demonstrated that high RhoA activity

55 We assembled four plasmid constructs, each containing a DNA fragment from

56 The assay utilized a plasmid construct encoding the cDNA for chloramphenicol

57 ses were detected only in mice injected with plasmid constructs encoding the core.

58 Compared with standard plasmid constructs, episomes encoding 107/402-T yield ap

59 The murine IFN-beta plasmid construct exhibited the greatest reduction, whil

60 of the genes for uPA and uPAR using a single plasmid construct expressing shRNAs for both uPA and uPA

61 strated that intratumoral injection with the plasmid construct expressing shRNAs for uPA and uPAR alm

62 These investigations demonstrate that plasmid constructs expressing HCV core protein generate

63 ear localization signal was identified using plasmid constructs expressing truncation mutants of the

64 own-regulated by the stable integration of a plasmid-construct expressing antisense-MUC4 RNA.

65 splicing of these modified oligomers into a plasmid construct followed by transfection into mouse em

66 Expression of the plasmid construct following topical application in the e

67 Our reagents include a plasmid construct for making custom TAL effectors and on

68 Transient cell transfection assays, using plasmid constructs harboring the perlecan promoter linke

69 ng reporter genes in transiently transfected plasmid constructs implicated a conserved intron found i

70 co-expressed with an MT2a promoter-reporter plasmid construct in human intestinal Caco-2 cells, indi

71 arabinose-inducible pBAD/Myc-HisA expression plasmid constructed in our laboratory.

72 Using a series of transiently transfected plasmid constructs in which gene segments of the prothro

73 Transient cell transfection experiments with plasmid constructs in which the potential AP-1-binding s

74 A CD2-EGFP plasmid construct including the CD2 promoter, the EGFP t

75 ells that expressed AQP2 promoter-luciferase plasmid constructs, indicating that TonEBP influences AQ

76 of Agrobacterium cells carrying appropriate plasmid constructs into tobacco leaves in planta, reprod

77 sion results from hybrid and chimeric enzyme plasmid constructs, made with large and small subunit ge

78 cts from clostridial transformants harboring plasmid constructs of thlP-sNTR and abrBP-sNTR showed th

79 Two plasmid constructs, one containing stxB1 under the contr

80 btained following transfection with a hybrid plasmid construct (pAP-R2) containing the Neo resistance

81 ealed by transient transfection studies with plasmid constructs (pDTD-1097CAT, XRE-CAT, and ARE-CAT)

82 -198 to +43 region) DNA fragment/pGL3-basic plasmid construct resulted in about 6-fold increased luc

83 Co-injection of antisense and sense plasmid constructs results in a partial rescue of the an

84 hat tumor cells stably transfected with this plasmid construct secrete beta-hCG in response to hypoxi

85 a rat hepatocyte iNOS promoter-reporter gene plasmid construct showed that IL-1beta-induced promoter

86 ction of an OPN promoter-luciferase reporter plasmid construct showed that promoter activity is incre

87 We design a single plasmid construct that combines the transposase and the

88 sulted in homologous integration of a pAN7.1 plasmid construct that contained a 1.1-kb fragment of th

89 ion with ParC in Escherichia coli by using a plasmid construct that contained the parC and parB genes

90 HeLa cells were transfected with plasmid constructs that carry the SIV or SIV-MuLV chimer

91 n factor-binding sites on the replication of plasmid constructs that contain the SV40 origin of repli

92 Thus we have made minigene plasmid constructs that encode oligonucleotide sequences

93 Using plasmid constructs that express an alpha-amanitin-resist

94 e deleted were independently introduced into plasmid constructs that express the complete NSP-ORF.

95 In this study we developed plasmid constructs that overexpress temperature sensitiv

96 We developed plasmid constructs that utilized either ribozyme-mediate

97 ol acetyltransferase (CAT) reporter gene and plasmid constructs that were stably maintained in Escher

98 on constants by inhibiting the cleavage of a plasmid constructed to contain a target sequence for the

99 tide macrolactones by using versions of each plasmid constructed to contain defined mutations.

100 f the entire bacterial vector sequences from plasmid constructs to create supercoiled gene expression

101 Plasmids constructed to express the open reading frame i

102 Creation of the plasmid constructs, transformation of the A. tumefaciens

103 proteins can actively destabilize other parS-plasmid constructs under similar conditions.

104 The plasmid construct was confirmed by DNA sequencing; in vi

105 Using plasmid constructs we demonstrate that the level of TFO-

106 oramphenicol acetyltransferase reporter gene plasmid constructs, we determined that the region betwee

107 Using various gC plasmid constructs, we show that ICP27's stimulatory eff

108 These plasmid constructs were then transcribed in vitro, and t

109 Next, the nonreplicative plasmid constructs were transformed by electroporation i

110 The new production system uses a plasmid construct which contains a mini-Ad genome capabl

111 tudied in a series of simian virus 40 (SV40) plasmid constructs whose relative replication efficiency

112 The plasmid construct with NF-kappaB element in place of the

113 d immunosorbent assay and was highest in the plasmid construct with stxB1 under the control of the ta