ライフサイエンスコーパス: pollen tube

1 on at the interface of the synergid cell and pollen tube.

2 ll and, thus, to the polarized growth of the pollen tube.

3 ain and are delivered to female gametes by a pollen tube.

4 mmotile sperm are delivered to an ovule by a pollen tube.

5 elivery of the sperm cells to the ovule by a pollen tube.

6 pe) can inhibit the subsequent growth of the pollen tube.

7 required to sustain the high growth rates of pollen tubes.

8 ments were less organized in myo11c1 myo11c2 pollen tubes.

9 y, actin organization, and optimal growth of pollen tubes.

10 specifically enriched in in vivo-elongating pollen tubes.

11 f Ca(2+) oscillations in the polar growth of pollen tubes.

12 tein kinase, CPK32, controls polar growth of pollen tubes.

13 e extracellular matrix inhibit interspecific pollen tubes.

14 ermination, and penetration of the stigma by pollen tubes.

15 mination of pollen grains, and the growth of pollen tubes.

16 as localized to the endoplasmic reticulum of pollen tubes.

17 pollen tubes and in lily (Lilium formosanum) pollen tubes.

18 cell wall at the tip of both root hairs and pollen tubes.

19 YVADase activity in mitochondria of growing pollen tubes.

20 anization critical for tip-focused growth in pollen tubes.

21 basis for S-haplotype-specific rejection of pollen tubes.

22 degeneration and repulsion of supernumerary pollen tubes.

23 ne trafficking and the actin cytoskeleton in pollen tubes.

24 mediated endocytosis and polar tip growth in pollen tubes.

25 complemented the aberrant growth of exo70C2 pollen tubes.

26 (4,5)P2] is critical for polar tip growth of pollen tubes.

27 et foci at the shank membrane of Arabidopsis pollen tubes.

28 ulation of optimal tip growth of Arabidopsis pollen tubes.

29 lementation at the apical plasma membrane of pollen tubes.

30 ls could not sustain the growth of wild-type pollen tubes.

31 ylinositol 4,5-bisphosphate (PIP2) marker in pollen tubes.

32 tubes, whereas NIP4;2pro:GUS flowers only in pollen tubes.

33 d by straight-billed territorial birds (mean pollen tubes = 0.20 +/- 0.074 SE) or insects.

34 ingbird species with specialized bills (mean pollen tubes = 1.21 +/- 0.12 SE) but was reduced 5.7 tim

35 ess this hypothesis, we employed the growing pollen tube, a well-established cell polarity model syst

36 llination of Heliconia tortuosa (measured as pollen tube abundance) occurred frequently when plants w

37 Different combinations of the timing of pollen-tube access to limiting stylar resources (simulta

38 address the question of how the tip-growing pollen tube achieves its rapid rate of elongation while

39 myb triple-mutant pollen tubes also fail to release their sperm cargo.

40 plants via ALMT, including the regulation of pollen tube and root growth, and that GABA can finally b

41 atus, the first point of contact between the pollen tube and the female gametophyte.

42 ell-to-cell communication events between the pollen tube and the female reproductive tissues, which a

43 zed cells flanking the egg cell that attract pollen tubes and degenerate upon pollen tube arrival.

44 dopsis (Arabidopsis thaliana) root hairs and pollen tubes and in lily (Lilium formosanum) pollen tube

45 d-pollination assay that can detect multiple pollen tubes and multiple sets of sperm within a single

46 at embryo sacs sense the imminent arrival of pollen tubes and respond by generating an oxidative envi

47 Pollen tubes and root hairs are model cell systems for s

48 Tip growth of pollen tubes and root hairs occurs via rapid polar growt

49 ules that multiplied to confer tip growth in pollen tubes and root hairs, respectively.

50 amics of HG during elongation of Arabidopsis pollen tubes and root hairs.

51 ing question for various cell types, such as pollen tubes and root hairs.

52 rol of cell size, polarity and the growth of pollen tubes and root hairs.

53 P. patens tip-growing cells and A. thaliana pollen tubes and root hairs.

54 enzyme was present in the growing region of pollen tubes and that the enzyme colocalizes with cell w

55 evelopment that strikingly resembles that of pollen tubes and their intrasexual competition within th

56 communication between the male gametophyte (pollen tube) and the maternal tissues of the flower, cul

57 cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the veg

58 localized in the cytoplasm of pollen grains, pollen tubes, and also root trichoblast cells.

59 ently associated with specific inhibition of pollen tubes, and its requirement for II was confirmed t

60 growing cells, such as animal neurons, plant pollen tubes, and root hairs.

61 th oscillations exhibited by rapidly growing pollen tubes; and its prediction of the observed phase r

62 ntrolled by the cortical actin fringe at the pollen tube apex.

63 nergid cells after physical contact with the pollen tube apex.

64 vered to ovules, as many as three additional pollen tubes are attracted.

65 enhanced maternal selection among competing pollen tubes are considered to have been major contribut

66 Furthermore, HT-B levels within the pollen tubes are reduced when NaStEP-suppressed pistils

67 hat attract pollen tubes and degenerate upon pollen tube arrival.

68 ppears to be processed and released from the pollen tube as an active peptide.

69 Using pollen tubes as a developmental model, we showed that th

70 A inhibited clathrin-mediated endocytosis in pollen tubes associated with an increased accumulation o

71 When gamete fusion fails, one of two pollen tube-attracting synergid cells persists, enabling

72 NIA (FER) and MYB98, which are necessary for pollen tube attraction and perception, remain unaffected

73 develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae.

74 identification of genes important in in vivo pollen tube biology.

75 including micropylar guidance (IV6 and IV4), pollen tube burst (IV2), and repulsion of multiple polle

76 hort [Ca2+]cyto transient is associated with pollen tube burst and sperm cell arrival.

77 female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations.

78 fertility caused by the rescue of anx1 anx2 pollen tube bursting.

79 taken up by both compatible and incompatible pollen tubes, but its suppression in Nicotiana spp. tran

80 ells had lower arabinose levels in roots and pollen tubes, but no differences were observed in GalA o

81 hored protein implicated in reception of the pollen tube by the female gametophyte.

82 ated MYB transcription factors is induced in pollen tubes by growth in the pistil.

83 s report, we formulate a simple mechanism in pollen tubes by which deposition causes turnover of cell

84 rs after intensive communication between the pollen tube cell and the receptive synergid, culminating

85 tility and are transported by the vegetative pollen tube cell for fertilization, but the extent to wh

86 ire transportation as a passive cargo by the pollen tube cell to the egg apparatus (egg cell and acce

87 longitudinal orientation in the Arabidopsis pollen tube cell wall, consistent with a linear arrangem

88 cell wall polymers composing the Arabidopsis pollen tube cell wall.

89 te at the filiform apparatus, where arriving pollen tubes communicate with the embryo sac.

90 The walls of Nicotiana alata pollen tubes contain a linear arabinan composed of (1,5)

91 However, exo70C2 pollen tubes could frequently recover and restart their

92 ically expressed and delivered LRE-cYFP from pollen tubes could non-cell-autonomously complement the

93 In the pollen tube, cytoplasmic TGases are likely to be involve

94 ted exocytosis-related functional effects in pollen tubes despite the absence of apparent plasma memb

95 entification of novel compounds that altered pollen tube development and endomembrane trafficking.

96 the mutant allele resulting from defects in pollen tube development, indicating that TAF1 is importa

97 that sperm cells are dispensable for normal pollen tube development.

98 in actinobacteria and filamentous fungi and pollen tube development.

99 or wild-type stigmas, most of the resulting pollen tubes did not extend into the transmitting tract,

100 nucleus during growth in the pistil leads to pollen tube differentiation required for release of sper

101 known of the transcriptome of in vivo-grown pollen tubes due to the difficulty of collecting pollen

102 is required for the stability of HT-B inside pollen tubes during the rejection response, but the unde

103 has been used to identify genes expressed in pollen tubes elongating in vitro; however, little is kno

104 errelated, and all of them are essential for pollen tube elongation in pistil, especially, free Ca(2+

105 Inhibition of pollen tube elongation was reversible if peptide was rem

106 nous SlPRALF acts as a negative regulator of pollen tube elongation within a specific developmental w

107 cific PMEs are traditionally associated with pollen tube elongation, this study provides strong evide

108 EI4 and AtPMEI9 had distinct consequences on pollen tube elongation.

109 nation of metabolites during germination and pollen tube elongation.

110 may modify the pollen cell wall and regulate pollen tube emergence and growth.

111 en attachment site, for pollen hydration and pollen tube entry.

112 their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain polle

113 We define a suite of pollen tube-expressed genes regulated by these critical

114 Additionally, pollen tube-expressing LRE variants lacking domains crit

115 horylation of purified recombinant PIP5K6 by pollen tube extracts could be attributed to MPK6.

116 Wild-type pollen tubes failed to arrest growth and to rupture afte

117 Furthermore, chx21 chx23 pollen tubes failed to enter the micropyle of excised ov

118 eplaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregula

119 peptides as candidate molecular mediators of pollen tube-female interactions necessary for flowering

120 persists, enabling the ovule to attract more pollen tubes for successful fertilization.

121 A mechanism preventing multiple pollen tubes from entering an ovule would ensure that on

122 at Arabidopsis efficiently prevents multiple pollen tubes from entering an ovule.

123 anslation (the translatome) of in vivo-grown pollen tubes from self-pollinated gynoecia of Arabidopsi

124 Our work defines the pollen tube gene products that respond to the pistil and

125 In particular, myo11c1 myo11c2 pollen tubes grew more slowly than wild-type pollen tube

126 g compatible pollination of the angiosperms, pollen tubes grow in the pistil transmitting tract (TT)

127 Mutant pollen tubes grown in vitro exhibited an enhanced growth

128 After a pollen tube grows into the style, the S-RNases produced

129 Effects of PIP5K6 expression on pollen tube growth and cell morphology were attenuated b

130 ses to biotic and abiotic stress, as well as pollen tube growth and fertility.

131 roduction, which fuels single nucleus-driven pollen tube growth and is essential for plant reproducti

132 ere, we show that mat3 mutants have impaired pollen tube growth and reduced seed set.

133 reeding system was determined by analysis of pollen tube growth and seed production from controlled p

134 sion and guidance for pollen germination and pollen tube growth are abundantly present in the extrace

135 Pollen tube growth assays in vitro and in the pistil dem

136 Ca(2+) availability partially suppresses the pollen tube growth defects, suggesting that LRX proteins

137 tween SI and UI in the tomato clade, in that pollen tube growth differs between these two rejection s

138 e by examining seed set, pollen fitness, and pollen tube growth for knockout mutants of five of the s

139 ulted in reduced pollen adhesion and delayed pollen tube growth in all mutants studied.

140 abacum resulted in the loss of inhibition of pollen tube growth in Nicotiana obtusifolia (synonym Nic

141 s characterized by evaluating N. obtusifolia pollen tube growth in normal and TT-ablated N. tabacum s

142 pollen development, pollen germination, and pollen tube growth in other species.

143 nscript levels during stamen development and pollen tube growth in the transgenic trees of a stamen-s

144 s vital and in vitro germination normal, but pollen tube growth inside stylar tissues appeared less d

145 Pollen tube growth is an essential aspect of plant repro

146 Pollen tube growth is central to the sexual reproduction

147 In flowering plants, pollen tube growth is dependent on the actin cytoskeleto

148 Pollen tube growth is rapid, occurs exclusively at the t

149 ion of the exocytotic machinery depending on pollen tube growth modes.

150 , a sugar alcohol, in flower development and pollen tube growth of apple (Malus domestica).

151 tigmas, pollen development, pollination, and pollen tube growth require spatial and temporal regulati

152 Inhibition of N. obtusifolia and N. repanda pollen tube growth required accumulation of PELPIII in t

153 h predictions made by a mechanical model for pollen tube growth revealed the importance of pectin dee

154 Pollen Receptor-Like Kinases (PRKs) control pollen tube growth through the pistil in response to ext

155 ing or the actin cytoskeleton, then examined pollen tube growth using fluorescent protein markers tha

156 an essential role in stamen development and pollen tube growth via MdMYB39L in apple.

157 e TT slowed and then arrested N. obtusifolia pollen tube growth, and was developmentally synchronized

158 ered clearly from the metabolic state during pollen tube growth, as indicated by principal component

159 Polarized exocytosis is critical for pollen tube growth, but its localization and function ar

160 ignaling network, resulting in inhibition of pollen tube growth, cytoskeletal alterations, and progra

161 use severe defects in pollen germination and pollen tube growth, resulting in a reduced seed set.

162 its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes

163 ecreased pollen germination rate and reduced pollen tube growth, which were all closely related to lo

164 luble inorganic pyrophosphatase required for pollen tube growth.

165 imilar pollen myosins had greater defects in pollen tube growth.

166 ect leading to more severe depolarization of pollen tube growth.

167 l-associated TGases are believed to regulate pollen tube growth.

168 reduced germination efficiency, and reduced pollen tube growth.

169 erspecific) pollen through the inhibition of pollen tube growth.

170 ss effects on pollen hydration, adhesion and pollen tube growth.

171 expression of its putative target genes and pollen tube growth.

172 ze the mitochondria and contribute to arrest pollen tube growth.

173 ect (segregation 40%:51%:9%) due to aberrant pollen tube growth.

174 ough the male gametophyte due to a defect in pollen tube growth.

175 pollen, while NIP4;2 expression peaks during pollen tube growth.

176 processes and sexual reproduction, namely in pollen tube guidance and embryo sac fertilization, patho

177 es the high robustness and responsiveness of pollen tube guidance and explains the connection between

178 a(2+) providing a concentration gradient for pollen tube guidance and involved in signal transduction

179 by the endogenous promoter caused disturbed pollen tube guidance and reduced fertility.

180 issues of the flower, culminating in precise pollen tube guidance to the female gametophyte (embryo s

181 t1 mutants show severe dwarfism, compromised pollen tube guidance, and constitutive activation of sal

182 oscopy revealed that tt16 deficiency affects pollen tube guidance, resulting in reduced fertility and

183 thaliana, CHX21 and CHX23, are essential for pollen tube guidance.

184 lassical AGP function as a Ca(2+) capacitor, pollen tube guide and wall plasticizer into a simple but

185 ll of the Arabidopsis (Arabidopsis thaliana) pollen tube has not been described in detail.

186 ility but inhibited the elongation of normal pollen tubes in an in vitro growth system.

187 tube burst (IV2), and repulsion of multiple pollen tubes in embryo sac (IV2).

188 By manipulating the [pH]cyt of the pollen tubes in vivo, we show that [pH]cyt acidification

189 OP1 GTPase, a key regulator of tip growth in pollen tubes, in an opposite manner.

190 MeSA, are localized at the apical region of pollen tubes, indicating of the tip-localized production

191 pollen grains disrupts the SI by preventing pollen tube inhibition.

192 rgid cell and the coincident bursting of the pollen tube inside the ovule to release the sperm.

193 tube-expressed and are required to maintain pollen tube integrity.

194 In pollen tubes, interaction between RAC5 and RISAP is dete

195 The pollen tube is a cellular protuberance formed by the pol

196 A pollen tube is a single cell that only grows at the tip,

197 amnogalacturonan I, the arabinan in N. alata pollen tubes is considered free, as there is no detectab

198 rate that the growth guidance of Arabidopsis pollen tubes is regulated by the signaling network that

199 brane trafficking and inhibits exocytosis in pollen tubes; it also leads to the degradation of the ac

200 y to precisely reproduce the morphologies of pollen tubes; its prediction of the growth oscillations

201 Pollen tubes lacking these three transcriptional regulat

202 r silique and reduced pollen germination and pollen tube length.

203 Root hair cells and pollen tubes, like fungal hyphae, possess a typical tip

204 The intensification of pollen tube (male gametophyte) competition and enhanced

205 StEP and NaSIPP interaction, in incompatible pollen tubes, might destabilize the mitochondria and con

206 the model captures the rich phenomenology of pollen tube morphogenesis and has implications for other

207 593 to the globular tail domain of a tobacco pollen tube myosin XI.

208 a indicate that de novo transcription in the pollen tube nucleus during growth in the pistil leads to

209 Sperm cell release from the pollen tube occurs after intensive communication between

210 ell wall of the pollen grain and the growing pollen tube of plasmolyzed pollen.

211 wth by oversecretion of CW material, whereas pollen tubes of anx1 anx2 double mutants burst spontaneo

212 novel, as LRE was not expressed in pollen or pollen tubes of mutants defective for MET1, DDM1, RNA-de

213 r to pollination, the growth of incompatible pollen tubes of N. obtusifolia and N. repanda was inhibi

214 Our results show that in root hairs, as in pollen tubes, oscillatory peaks in PI fluorescence prece

215 mics analyses confirmed that mat3 pollen and pollen tubes overaccumulate Met and that mat3 pollen has

216 r guided growth of the male gametophytes and pollen tube-ovule interaction.

217 ne acts in pollen development in anthers and pollen tube passage in the carpels.

218 In lily (Lilium formosanum) pollen tubes, pectin, a major component of the cell wall

219 expression, from first pollen mitosis up to pollen tube penetration in the ovule, thereby revealing

220 which are major human allergens, facilitate pollen tube penetration of the maize stigma and style (t

221 of pollen grain adhesion, pollen hydration, pollen tube penetration, seed set, and overall fertility

222 oforms localized to different regions of the pollen tube plasma membrane, apical vesicle-rich inverte

223 ions at the tonoplast, nuclear membrane, and pollen tube plasma membrane, emphasizing the role of thi

224 ,5)P2 production and membrane trafficking in pollen tubes, possibly contributing to directional growt

225 len tubes, where potential interactions with pollen tube proteins might underlie its function.

226 between synergid cells and a tip-elongating pollen tube (PT) for the successful delivery of sperm ce

227 One of the two sperm delivered by the pollen tube (PT) fuses with the egg cell to form the zyg

228 ubfamily, are required for tip growth of the pollen tube (PT), and their closest homolog, FERONIA, co

229 Apical growth in pollen tubes (PTs) is associated with the presence of ti

230 are self-sterile despite the fact that self-pollen tubes reach the ovary and in most cases penetrate

231 cell surface and known to critically control pollen tube reception [6].

232 erefore, LRE and FERONIA jointly function in pollen tube reception at the interface of the synergid c

233 l-anchored membrane protein, is critical for pollen tube reception by the female gametophyte before f

234 In angiosperms, pollen tube reception by the female gametophyte is requi

235 s could non-cell-autonomously complement the pollen tube reception defect in lre female gametophytes,

236 transmembrane domain, fully complemented the pollen tube reception defect in lre-7 female gametophyte

237 les that remain undeveloped due to defective pollen tube reception did not induce synergid degenerati

238 Arabidopsis thaliana lorelei (lre) mutants, pollen tube reception fails in most female gametophytes,

239 Several important factors involved in the pollen tube reception have been identified recently [2-6

240 al female signaling components essential for pollen tube reception have been identified; however, mal

241 ring plants, the female gametophyte controls pollen tube reception immediately before fertilization a

242 t of autonomous endosperm development in the pollen tube reception mutants scylla and sirene.

243 sing LRE-cYFP showed that the role of LRE in pollen tube reception requires the M8CM, but not the dom

244 most lre-5 female gametophytes do not allow pollen tube reception, in those that do, early seed deve

245 ent, inefficient SC delivery, and defects in pollen tube reception.

246 proteins (ENODLs, or ENs), are required for pollen tube reception.

247 role of ENs in male-female communication and pollen tube reception.

248 metophyte induces rupture of the penetrating pollen tube, resulting in sperm release and rendering th

249 YVADase activity using live-cell imaging of pollen tubes revealed YVADase activity in mitochondria o

250 cals in the female gametophyte, which causes pollen tube rupture and sperm cell release during fertil

251 entrance to the female gametophyte to induce pollen tube rupture and sperm release.

252 BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.

253 t reactive oxygen molecules, and they induce pollen tube rupture in a Ca(2+)-dependent process involv

254 ts, whose female gametophytes fail to induce pollen tube rupture, to decipher the molecular mechanism

255 dy-state growing tobacco (Nicotiana tabacum) pollen tubes, SEC3a displayed amino-terminal Pleckstrin

256 Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of M

257 n the model tip growth system of Arabidopsis pollen tubes, several small-molecule hormones regulate t

258 zes to punctae at the plasma membrane of the pollen tube shank, which are stably associated with cort

259 Coexpression of NaStEP and NaSIPP in pollen tubes showed interaction in the mitochondria, alt

260 Upon arrival at the female gametophyte, the pollen tube stops growing and releases sperm cells for s

261 or after) create signature relations of mean pollen-tube success and its variation among pistils to p

262 molecules interacting with receptors at the pollen tube surface.

263 germination, the vegetative cell extrudes a pollen tube that carries the sperm to an ovule for ferti

264 s at the tip and along the shank of N. alata pollen tubes that are predominantly part of the primary

265 ng male sterility defect as a consequence of pollen tubes that fail to fully elongate following polli

266 ity to exert penetrative forces generated in pollen tubes, the fastest-growing plant cells.

267 self S-RNases, but not self S-RNases, in the pollen tube, thereby resulting in cross-compatible polli

268 expansins in promotion of penetration of the pollen tube through the stigma and style, most likely by

269 creasing mechanical impedance was met by the pollen tubes through modulation of the cell wall complia

270 zation in angiosperms requires the growth of pollen tubes through the female reproductive tract as th

271 ch has identified key components involved in pollen tube tip bursting that allow sperm release and fe

272 Occam's Razor suggests a new model of pollen tube tip growth based on a novel Hechtian oscilla

273 In tobacco (Nicotiana tabacum), pollen tube tip growth is controlled by the RAC/ROP GTPa

274 was during germination and the initiation of pollen tube tip growth.

275 mpartment, whose cytoplasmic position at the pollen tube tip is maintained by the actin cytoskeleton.

276 tional and accumulates at or proximal to the pollen tube tip plasma membrane.

277 ead being delivered in a wide arc across the pollen tube tip.

278 d-labeled vesicles and their accumulation at pollen tube tips were not affected in the myo11c1 myo11c

279 ccessful female gametophyte will mate with a pollen tube to produce an embryo and an endosperm.

280 that are required for sperm release from the pollen tube to the female gametes, a critical barrier to

281 maximum reproductive success by distributing pollen tubes to all ovules.

282 self-propelled sperm cells (SCs), plants use pollen tubes to deliver SCs, in which the pollen vegetat

283 hat, coordinately, allow root hair cells and pollen tubes to expand in a controlled manner to several

284 Species-preferential proteins attract pollen tubes to female gametes in flowering plants.

285 C S. pennellii LA0716 allows S. lycopersicum pollen tubes to penetrate farther into the pistil in HT

286 SC S. arcanum LA2157 allows S. lycopersicum pollen tubes to penetrate to the ovary and produce hybri

287 owering plants, sperm are transported inside pollen tubes to the female gametophyte for fertilization

288 tarch and other carbohydrates similar to the pollen tube transmitting tissues in the styles of most f

289 intrasexual competition within the maternal pollen tube transmitting tissues of most flowers.

290 ns as well as in vivo- and in vitro-cultured pollen tubes were assayed by microarray analyses, reveal

291 ed in the cell wall of pear (Pyrus communis) pollen tubes, where it might play a role in the regulati

292 During pollination, NaStEP is taken up by pollen tubes, where potential interactions with pollen t

293 ers showed GUS activity in mature pollen and pollen tubes, whereas NIP4;2pro:GUS flowers only in poll

294 he stigma result in the polar outgrowth of a pollen tube, which delivers sperm cells to the female ga

295 xogenous pectin methyl esterase treatment of pollen tubes, which demethoxylates pectins, freeing more

296 pollen tubes grew more slowly than wild-type pollen tubes, which resulted in reduced fitness compared

297 asma membrane in the shank region of growing pollen tubes, which we have termed Actin-Membrane Contac

298 zed as the site of exocytosis in the tobacco pollen tube, while NtEXO70B1 surprisingly colocalized wi

299 is more complex, involving interaction of a pollen tube with egg adjoining synergid cells, culminati

300 ion of transcripts engaged with polysomes in pollen tubes within self-fertilized florets has identifi