ライフサイエンスコーパス: poly (A) mRNA

1 ase chain reaction analysis of human cardiac poly A+ mRNA.

2 sequences, we demonstrated the presence of a poly(A) mRNA.

3 tion in the first step of the degradation of poly(A) mRNA.

4 hows little enhancement of expression of non-poly(A) mRNA.

5 plied to primer extension analysis of kidney poly(A) mRNA.

6 proteins, or the cytoplasmic localization of poly(A) mRNA.

7 each of which derepresses translation of non-poly(A) mRNA.

8 ncomitant increase in dendritic and synaptic poly(A) mRNA.

9 nd observing a concomitant decrease in total poly(A)+ mRNA.

10 from poly(A)- mRNA but had little effect on poly(A)+ mRNA.

11 l beta2 subunit were cloned from human heart poly(A)(+) mRNA.

12 RNA is described, separating mouse rRNA from poly(A)(+) mRNA.

13 pap1-1 mutation results in reduced levels of poly(A)(+) mRNAs.

14 inguish between cap(+)/poly(A)(+) and cap(-)/poly(A)(-) mRNAs.

15 Slt2 is required for nuclear poly(A(+)) mRNA accumulation upon heat shock, and thermo

16 Translation of poly(A)- mRNAs after polyadenylation shut-off might resu

17 , we found that sympathetic axons do contain poly(A+) mRNA along their length in a pattern that chang

18 both these changes and increases in tubulin poly A+ mRNA and protein coexist indefinitely after a ne

19 between the polyadenylation machinery, newly poly(A) mRNAs, and factors for transcript export.

20 We show that significant amounts of poly(A)+ mRNAs are oxidized in AD brains.

21 7 mutation derepresses the expression of non-poly(A) mRNA as much as a quadruple ski2 ski3 ski7 ski8

22 nificant percentage of poly(A)-deficient and poly(A)- mRNA associate with smaller polyribosomes compa

23 actions of HuR with beta-actin mRNA and with poly(A)+ mRNA at both native and increased HuR expressio

24 ng the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature.

25 First, we show that both poly(A)(-) and poly(A)(+) mRNA become translationally repressed during

26 ts with poly(A) polymerase and with Npl3p, a poly(A)(+) mRNA binding protein implicated in pre-mRNA p

27 ified Sec61alpha,beta and ribophorin I as ER-poly(A) mRNA-binding proteins, suggesting unexpected rol

28 ength of the 3'UTR increased expression from poly(A)- mRNA but had little effect on poly(A)+ mRNA.

29 the presence of normal amounts of competing poly(A)(+) mRNA, but is normally prevented from doing so

30 hat Ski2p and Slh1p block translation of non-poly(A) mRNA by an effect on Fun12p, possibly by affecti

31 Herein, we provide evidence that poly(A)(+) mRNAs can enter P-bodies in yeast.

32 and defense compounds, northern analysis of poly(A)+ mRNA demonstrates that transcripts encoding CYP

33 cation-dependent (RD) histone genes generate poly(A)- mRNA encoding new histones to package DNA durin

34 molog of the yeast Gle1 involved in the same poly(A) mRNA export pathway as Nup159, also result in se

35 tify 72 factors required for polyadenylated [poly-(A(+))] mRNA export from the nucleus in Drosophila

36 due to a twofold-increased repression of non-poly(A) mRNA expression.

37 cloned by reverse transcription of juice-sac poly(A)+ mRNA, followed by Taq Polymerase-mediated ampli

38 Arrays were then probed with poly (A) mRNA from single, microdissected dysplastic neu

39 ter (EAAC1) cDNAs were probed with amplified poly (A) mRNA from tubers or normal neocortex to identif

40 d with probes to TIMP-1 to -4 and GAPDH with poly A+ mRNA from ventricular tissues of patients with i

41 oligo(dt) magnetic bead protocol to harvest poly(A) mRNA from cultured cells in 96-well plates minim

42 In screening amplified poly(A) mRNA from hippocampal dendrites and growth cones

43 AR1 is transcribed as an 800-nucleotide (nt) poly(A)+ mRNA from a promoter lacking a consensus TATA s

44 Northern blot analysis of poly(A)+ mRNA from N. benthamiana and N. tabacum cv. MD6

45 t1 showed a single band of approx. 1.6 kb in poly(A)+ mRNAs from epidermis, limb bud or claw muscle a

46 In some cells, RD H2A.X poly(A)- mRNA generates sufficient histone for depositio

47 ation partially slows elongation, permitting poly(A) mRNA in the ribosome's decoding center to adopt

48 perature and partially restore the levels of poly(A)(+) mRNA in a manner distinct from the cytoplasmi

49 h Pab1p does not diminish the preference for poly(A)(+) mRNA in vivo, indicating another role for pol

50 Because of the absence of poly(A)+ mRNA in prokaryotic organisms, studies of diffe

51 used accumulation of active spliceosomes and poly(A)+ mRNAs in unique splicing clusters located at th

52 protein is localized at the nuclear rim, and poly(A)-mRNA in situ hybridization shows that mRNA expor

53 nslation, allowing better translation of non-poly(A) mRNA, including the L-A virus mRNA which lacks p

54 We now demonstrate that Ram-1 poly(A)+ mRNA increases significantly following culture

55 Northern analyses of poly(A) mRNA indicated two major species of about 8 and

56 ay facilitate the localization of associated poly(A) mRNAs into axons.

57 o not bind sperm, but injection of total egg poly(A)+ mRNA into immature oocytes confers sperm bindin

58 late that the derepressed translation of non-poly(A) mRNAs is due to abnormal (but full-size) 60S sub

59 use (P:D ratio) of the resulting cytoplasmic poly(A)+ mRNA is a measure of poly(A) site strength.

60 d histone gene, generating both poly(A)+ and poly(A)- mRNA isoforms.

61 Northern analysis with poly (A)+ mRNA isolated from different developmental sta

62 a specific reduction of both HuD protein and poly(A) mRNA levels in the axonal compartment.

63 mance: specificity of 1:250,000 in mammalian poly(A(+)) mRNA; limit of detection 0.13 pM; dynamic ran

64 We suggest that rapid degradation of non-poly(A) mRNAs may be primarily responsible for the shape

65 In this study, we developed an engineered poly(A) mRNA mimic, AAAAAAAAAAAA, named A12, with 2'-O-m

66 Poly(A) mRNA-mRNP complexes were purified from a postmit

67 : the intracellular distribution of cellular poly(A)(+) mRNA, nuclear proteins, and, most important,

68 ski8 mutations enhance the expression of the poly(A)(-) mRNAs of yeast RNA viruses.

69 nter P-bodies, and an mRNP complex including poly(A)(+) mRNA, Pab1p, eIF4E, and eIF4G2 may represent

70 romoting translation from poly(A)(+) but not poly(A)(-) mRNAs, particularly for mRNAs containing seco

71 se the accumulation of the secretory form of poly(A)(+) mRNA relative to the membrane form and regula

72 In contrast, cells making predominantly poly(A)+ mRNA require this isoform for de novo H2A.X syn

73 much higher concentrations of yeast tRNA or poly(A)mRNA, respectively, 33- and 60-fold greater than

74 ors generated from hippocampal and forebrain poly(A)+ mRNA revealed greater sensitivity to 2,3-benzod

75 Northern analysis of poly(A+) mRNAs reveals two differently sized rnp-4f mRNA

76 ms of a specific mRNA showed relatively more poly(A)- mRNA sedimenting with 20-60 S complexes than do

77 ing, specifically reduces the translation of poly(A)(+) mRNA, suggesting that poly(A) may have a role

78 f p53 and to the inhibition of total RNA and poly(A) mRNA synthesis.

79 loss-of-function mutant, we showed that XRN4 poly(A(+)) mRNA targets are largely subject to cotransla

80 oach we have isolated a single cDNA from egg poly(A)+ mRNA that can induce sperm binding in immature

81 pap1-1 mutation results in the synthesis of poly(A)- mRNAs that initiate translation with surprising

82 For poly(A)+ mRNA, the translational efficiency and mRNA hal

83 ryotes, the nucleocytoplasmic export of bulk poly(A)(+)-mRNAs through the nuclear pore complex is med

84 on-independent (RI) histone genes synthesise poly(A)+ mRNA throughout the cell cycle, translated into

85 Hybridization of poly(A)(+) mRNA to DNA microarrays containing 96.4% of y

86 of HYPB/Setd2, like Iws1, induced bulk HeLa poly(A)+ mRNAs to accumulate in the nucleus.

87 factors to mRNA, caused by the inability of poly(A)- mRNAs to accumulate to normal levels.

88 lse-labeling experiments indicate that total poly(A)(+) mRNA transcription was not significantly redu

89 Poly(A) mRNA was present in the nucleus and throughout t

90 tubers, we developed a methodology in which poly(A)+ mRNA was amplified from immunohistochemically l

91 ocesses, reverse transcription-PCR with PC-3 poly(A)+ mRNA was performed by using degenerate oligonuc

92 Poly(A)+mRNA was present in the axon tips, and was more

93 The total population of polyadenylated [poly(A)] mRNA was localized in hippocampus using a bioti

94 Poly-(A)+ mRNA was isolated for Northern blot analysis.

95 contrast, highly toxic 36R in the context of poly(A)(+) mRNA were exported to the cytoplasm, where DP

96 Tissue-specific libraries from poly(A)(+) mRNA were prepared by priming first and secon

97 sed the expression of nonpolyadenylated [non-poly(A)] mRNAs, whether capped or uncapped, thus explain

98 The labeled granules colocalized with poly(A+) mRNA, with the 60S ribosomal subunit, and with