ライフサイエンスコーパス: poly (A) tail

1 single polyadenylation site 229 nt from the poly-A tail.

2 capture of information distant from the mRNA poly-A tail.

3 leolytic cleavage followed by synthesis of a poly(A) tail.

4 codons or translational readthrough into the poly(A) tail.

5 tly translated in vivo despite the lack of a poly(A) tail.

6 nding that PUMs repress an mRNA that lacks a poly(A) tail.

7 e U-rich internal loop of the ENE and the 3'-poly(A) tail.

8 cription or translation or the status of the poly(A) tail.

9 capping by the addition of a CUCU tag to the poly(A) tail.

10 that hybridizes to and protects the PAN RNA poly(A) tail.

11 ling in ribosomes on translation through the poly(A) tail.

12 pended upon the mRNA's 5' cap but not its 3' poly(A) tail.

13 s end in a conserved stem-loop rather than a poly(A) tail.

14 t recognize the cis-elements and produce the poly(A) tail.

15 ndent on the length of both the mRNA and the poly(A) tail.

16 nterplay between the 5' m(7)G cap and the 3' poly(A) tail.

17 X(L) mRNA was dependent upon the presence of poly(A) tail.

18 PEB, but after polyadenylation, it binds the poly(A) tail.

19 g in the stem-loop but not a reporter with a poly(A) tail.

20 ot distinguish RNAs with or without a cap or poly(A) tail.

21 mRNAs in that they possess a 5' cap and a 3' poly(A) tail.

22 A-like intramolecular hybridization with the poly(A) tail.

23 p like those of cellular mRNAs but lack a 3' poly(A) tail.

24 expressed alpha-globin mRNA and a 72-residue poly(A) tail.

25 RNA and the subsequent deadenylation of the poly(A) tail.

26 lement (3' CSE) immediately preceding the 3'-poly(A) tail.

27 MKIIalpha protein and mRNA by shortening its poly(A) tail.

28 nd cytoplasmic lengthening of the neg-1 mRNA poly(A) tail.

29 n the renal outer medulla appeared to lack a poly(A) tail.

30 precursor, cleaves at that site, and adds a poly(A) tail.

31 pore complexes and the length of transcript poly(A) tails.

32 of single-stranded, positive-sense RNAs with poly(A) tails.

33 ust circadian rhythms in the length of their poly(A) tails.

34 these RNAs while stabilizing mRNAs with long poly(A) tails.

35 iduals were shown to have severely truncated poly(A) tails.

36 uncated AU-rich mRNAs lacking the 3' UTR and poly(A) tails.

37 nto structures previously thought to be long poly(A) tails.

38 A and mRNA; and the Distal Site, which binds poly(A) tails.

39 o fractions dependent on the length of their poly(A) tails.

40 ly(A) tails while other mRNAs to have longer poly(A) tails.

41 ed with maintenance and/or extension of long poly(A) tails.

42 4 with mRNAs containing critically shortened poly(A) tails.

43 translational repression by miRNAs require a poly(A) tail, a 3' histone stem-loop being an effective

44 processes, it is important to identify these poly(A) tails accurately in transcriptome sequencing dat

45 In eukaryotes, the poly(A) tail added at the 3' end of an mRNA precursor is

46 A poly(A) tail added by the normal 3'-processing machinery

47 of a targeted gene, subsequently inhibiting poly(A) tail addition and leading to degradation of that

48 on in most eukaryotes, involves cleavage and poly(A) tail addition at the 3' end of mRNAs at the poly

49 The presence of the exosome suppressed poly(A) tail addition, while TRAMP stimulated exosome de

50 of U1 snRNP to the target pre-mRNA inhibits poly(A)-tail addition, causing degradation of that RNA s

51 nscribed regions of mRNAs using 3'-ESTs with poly(A) tails aligned to genomic sequences.

52 ansport and translation, suggesting that the poly(A)-tail also provides a basis for eukaryotes to eff

53 cells accomplish similar decoupling through poly(A) tail alterations to ensure that dormant transcri

54 ymerase in the maintenance and repair of the poly(A) tail, an element required for replication of the

55 HTTAS is 5' capped, poly (A) tailed and contains three exons, alternatively

56 ells, the oocyte transcriptome has a shorter poly(A) tail and a higher relative proportion of termina

57 insertion of uridylate residues between the poly(A) tail and a non-wild-type 3' sequence can restore

58 down cells, p53 mRNA has an abnormally short poly(A) tail and a reduced translational efficiency, res

59 on of an mRNA is strongly impacted by its 3' poly(A) tail and associated poly(A)-binding proteins (PA

60 nteraction of topoisomerase IIalpha with the poly(A) tail and G/U-rich 3'-untranslated region (3'-UTR

61 nome of TV contains 6,714 nucleotides plus a poly(A) tail and is organized into three open reading fr

62 FLuc production rate that was dependent on a poly(A) tail and poly(A)-binding protein, but was indepe

63 tramolecular RNA clamp, sequestering the PAN poly(A) tail and preventing the initiation of RNA decay.

64 knockdown oocytes, Gdf9 RNA has a shortened poly(A) tail and reduced expression.

65 rotein(s) that simultaneously recognizes the poly(A) tail and small edited element and which blocks t

66 s between polysomes and P-bodies and how the poly(A) tail and the associated poly(A) binding protein

67 proximity of the termination codon to the 3' poly(A) tail and the poly(A) RNA-binding protein, PAB1,

68 nctionally replaced interactions between the poly(A) tail and the poly(A)-binding protein (PABP) to a

69 As: efficiently translated mRNAs have longer poly(A) tails and are shorter, more stable, and more eff

70 cells into two fractions with short and long poly(A) tails and compared them by microarray analysis.

71 ocol that queries the junctions of 3'UTR and poly(A) tails and confidently maps the poly(A) tags to t

72 form enhances translation and elongates the poly(A) tails and imparts its translational state to the

73 eotides to capture the RNAs at their natural poly(A) tails and initiate sequencing by synthesis.

74 either GLD-2 or RNP-8 resulted in shortened poly(A) tails and lowered abundance of four target mRNAs

75 length, while GC-rich Alus only in their 3' poly(A) tails and middle A-stretches, with differences d

76 ource tool for finding the precise border of poly(A) tails and other homopolymers in raw mRNA sequenc

77 levels of CDK11 increased the length of HIV poly(A) tails and the stability of mature viral transcri

78 ort a new technique to analyse the length of poly(A) tails and to separate a mixed population of mRNA

79 majority of these products contained intact poly(A) tails and were bound by the poly(A) binding prot

80 ion, independent of 7-methylguanylate cap or poly(A) tail, and prompts mRNA redistribution to silenci

81 inding protein N1 (PABPN1) and PABPC1 at the poly(A) tail, and, provided biogenesis involves pre-mRNA

82 nscripts with a high frequency of very short poly(A) tails, and a loss of 3' oligo-uridylation.

83 L1/MALAT RNAs accumulate in cells, lack poly(A) tails, and are translated; however, they cannot

84 a mice have mild histologic defects, shorter poly(A) tails, and evidence of mitochondrial damage.

85 ith much less starting material, the lack of poly(A)-tails, and the fact that the messages can be pol

86 he first study to reveal that TATA boxes and poly (A) tails are direct targets for BBR in its regulat

87 esent study demonstrates that TATA boxes and poly (A) tails are the first and second primary targets

88 following: (i) the wild-type 3' CSE and the poly(A) tail are required, (ii) the poly(A) tail must be

89 Poly(A) tails are 3' modifications of eukaryotic mRNAs t

90 mRNA poly(A) tails are important for mRNA stability and trans

91 Therefore, we propose that mRNA poly(A) tails are important in regulating protein synthe

92 Following the resumption of meiosis, the poly(A) tails are lengthened and translation ensues.

93 on of mRNA poly(A) tail length revealed that poly(A) tails are longer than normal in a tpa1Delta stra

94 new insights into how transcripts that lack poly(A) tails are stabilized and regulated and suggest t

95 Here we identify a terminal poly(A) tail as being important for a subset of intron e

96 rity of both forms requires the mRNA cap and poly(A) tail, as well as eIF4E, eIF4G, Pab1 and eIF3, an

97 ulatory regions as well as the poly adenine (poly (A)) tail at the mRNA terminus.

98 transcripts have a 5' monophosphate, lack a poly(A) tail at the 3' end, and contain no introns; thes

99 ssenger RNA (mRNA) does not terminate with a poly(A) tail at the 3'-end.

100 timulated hypothalamus shortens the AVP mRNA poly(A) tail at the same time as reducing transcript abu

101 d of the Rous sarcoma virus, just inside the poly(A) tail, at the same time the Gilbert group at Harv

102 We uncover a poly(A) tail-based regulatory mechanism that dynamically

103 The newly elongated poly(A) tail becomes bound by poly(A) binding protein (P

104 tic initiation factor 4E (eIF4E), eIF4G, and poly(A) tail-binding protein (PABP) that circularizes mR

105 cal structure that could not only shield the poly(A) tail but also serve as a scaffold for the assemb

106 ctor 4E (eIF4E) at the cap and PABPC1 at the poly(A) tail, but that lacks detectable EJCs and PABPN1.

107 ranscribed by Pol II and acquire 5' caps and poly(A) tails, but only mRNAs are translated into protei

108 Coating of the poly(A) tail by mtPABP1, however, did not lead to transc

109 We demonstrate that mRNA in yeast lacking a poly(A) tail can be destabilized by introduction of a pr

110 ion of mRNAs, consistent with alterations in poly(A) tail chain termination.

111 end in a stem-loop structure instead of the poly(A) tail characteristic of all other mature mRNAs.

112 Many plant viruses without 5' caps or 3' poly(A) tails contain 3' proximal, cap-independent trans

113 w dwarf virus RNA, lacking a 5' cap and a 3' poly(A) tail, contains a cap-independent translation ele

114 ion in myoblasts led to a shortening of mRNA poly(A) tails, demonstrating the cellular function of PA

115 ing proteins directly recruit PABP, in a non-poly(A) tail-dependent manner, to stimulate the small su

116 supports export and translation as well as a poly(A) tail does.

117 eadenylases are best known for degrading the poly(A) tail during mRNA decay.

118 ranscription can initiate upstream of the 3' poly-A tail during retrotransposon integration.

119 tion motif of HuD, the domain that binds the poly(A) tail, eliminated the branch-specific expression

120 iously shown to be necessary for cytoplasmic poly(A) tail elongation and translational activation of

121 The WISP protein is required for poly(A) tail elongation of bicoid, Toll, and torso mRNAs

122 Poly(A) tail elongation showed no apparent pauses during

123 ossessing poly(A), as well as to cytoplasmic poly(A) tail elongation.

124 We thus expect that a translated poly-A tail, encoding for positively charged lysines reg

125 rithms struggle to accurately identify these poly(A) tail end-points.

126 Poly(A) tails enhance the stability and translation of m

127 ally, we show that neither a 5' cap nor a 3' poly(A) tail enhances Sec incorporation.

128 While greater distances with the poly(A) tail exacerbate dependency on PABP for deadenyla

129 to 3' stem-loop structures not involving the poly(A) tail exhibit even longer half-lives.

130 rase WISPY is responsible for stage-specific poly(A) tail extension in the female germline.

131 ware of focus exclusively on the trimming of poly(A) tails, failing to provide the detailed informati

132 in part through the removal of PAB1 from the poly(A) tail following its self-association into multime

133 onadenylated RNA is sufficient to switch the poly(A) tail from a destabilizing to a stabilizing eleme

134 nterestingly, REF/Aly appears to protect the poly(A) tail from deadenylation, and REF/Aly-stabilized

135 engths have impeded greater understanding of poly(A)-tail function.

136 In 3' RACE, the poly(A) tail functions as a non-specific tag at the 3' e

137 In eukaryotes, poly(A) tails generally stabilize mature mRNAs, whereas

138 w dwarf virus mRNA, which lacks both cap and poly(A) tail, has a translation element (3'-BTE) in its

139 pair DNA hairpin attached to a 50-nucleotide poly-A tail (HP-A(50)) is threaded into an alphaHL chann

140 ulates HO mRNA and stimulates removal of its poly(A) tail (i.e. deadenylation).

141 polyadenylation or retain a reasonably long poly(A) tail if they are to return to the translating po

142 ternative transcription start sites and a 5' poly(A) tail in maize transcripts.

143 n vivo, arguing against a major role for the poly(A) tail in microRNA-mediated silencing.

144 n may be relevant, as the positioning of the poly(A) tail in mRNAs influences the length of the 3'-un

145 Cyclin B1 pre-mRNA acquires a long poly(A) tail in the nucleus that is subsequently shorten

146 earch for the enzymes responsible for adding poly(A) tails in Chlamydomonas and Arabidopsis organelle

147 dentified 213 transcripts that have extended poly(A) tails in Noc KO liver.

148 efining a new and unexpected role for 3' end poly(A) tails in the nuclear surveillance and turnover o

149 D(pol) that resulted in shorter or longer 3' poly(A) tails in virion RNA.

150 port that depletion of Glc7 causes shortened poly(A) tails in vivo and accumulation of phosphorylated

151 the first example of a cellular IRES that is poly(A) tail-independent.

152 t is a polyU sequence that can interact with poly(A) tails, inhibit the association of poly(A)-bindin

153 e eukaryotic initiation factor (eIF) 4G/PABP/poly(A) tail interaction is achieved instead through the

154 A 3' poly(A) tail is a common feature of picornavirus RNA gen

155 e primers are removed by exonuclease I and a poly(A) tail is added to the 3' end of the first-strand

156 The 3' poly(A) tail is important in messenger RNA stability and

157 The foreshortened poly(A) tail is maintained by poly(A) ribonuclease, whic

158 RNA (mRNA) translation initiation by the 3' poly(A) tail is mediated through interaction of poly(A)-

159 Subsequent ePAB binding to the poly(A) tail is necessary to protect the homopolymer fro

160 , because PARN is more active than Gld2, the poly(A) tail is short.

161 sequence motifs that signal the addition of poly(A) tails is essential to improved genome annotation

162 s are polyadenylated in the nucleus, and the poly(A)-tail is required for efficient mRNA export and t

163 a single-stranded binding region, such as a poly(A) tail, is present.

164 cDNA synthesis specifically toward the 3UTR/poly(A) tail junction of cellular RNA.

165 The alterations in poly(A) tail length accompanying elevated PDE12 expressi

166 In summary, we show that poly(A) tail length and 3' terminal uridylation have ess

167 ar PAPS isoforms control de novo synthesized poly(A) tail length and hence expression of specific sub

168 a function for Hnrnpa1 in the regulation of poly(A) tail length and translation of maternal mRNAs th

169 Our data argue that changes in poly(A) tail length are not a universal mechanism govern

170 In fact, changes in poly(A) tail length are not sufficient to account for PN

171 n of pgc is concurrent with extension of its poly(A) tail length but appears largely independent of t

172 NA show growth defects as well as defects in poly(A) tail length but do not accumulate poly(A) RNA in

173 onsequences of altered pre-mRNA splicing and poly(A) tail length control.

174 a suggest that NPM1 has an important role in poly(A) tail length determination and may help network 3

175 st, highly reproducible and minor changes in poly(A) tail length distribution are easily detected.

176 Therefore, even though the poly(A) tail length dynamics seen between genotypes may

177 cial for gene expression and perturbation of poly(A) tail length has been linked to a human neurodege

178 etic data indicate that dNab2 restricts bulk poly(A) tail length in vivo, suggesting that this functi

179 on in the heart, where controlled changes in poly(A) tail length influence mRNA translation.

180 mportantly, we found that the rhythmicity in poly(A) tail length is closely correlated with rhythmic

181 Poly(A) tail length is emerging as an important marker o

182 Here, we report the mechanism by which poly(A) tail length is regulated.

183 also needed during oogenesis to regulate the poly(A) tail length of dmos during oocyte maturation and

184 ial action of RNA binding proteins modulates poly(A) tail length of maternal mRNAs, leading to asymme

185 A)denylome" analysis, a method that measures poly(A) tail length of transcripts in a global manner, a

186 se transcripts do not exhibit rhythmicity in poly(A) tail length or steady-state mRNA level, despite

187 basis for understanding its function in both poly(A) tail length regulation and in the compaction of

188 ly(A) RNA binding protein, Nab2, facilitates poly(A) tail length regulation together with targeting t

189 mRNP organization and compaction as well as poly(A) tail length regulation.

190 This poly(A) tail length restriction is controlled by Mtr4p.

191 Examination of mRNA poly(A) tail length revealed that poly(A) tails are long

192 d translation, and enzymes that regulate the poly(A) tail length significantly impact protein profile

193 me, consistent with previous reports linking poly(A) tail length with nuclear RNA surveillance.

194 ces translation termination efficiency, mRNA poly(A) tail length, and mRNA stability.

195 Depletion of GLD4 not only reduced GLUT1 poly(A) tail length, but also GLUT1 protein.

196 define the function of PABPN1 in control of poly(A) tail length, little is known about the role of P

197 nsistent with a potential role in regulating poly(A) tail length, Tpa1p was also found to coimmunopre

198 r PNPase nor RNase II has any effect on tRNA poly(A) tail length.

199 ing to RNA is critical for proper control of poly(A) tail length.

200 acterization of ZC3H14 as a regulator of RNA poly(A) tail length.

201 nal mRNAs is regulated by dynamic changes in poly(A) tail length.

202 that modulates both mRNA nuclear export and poly(A) tail length.

203 r-expression enhances AVP mRNA abundance and poly(A) tail length.

204 Furthermore, although poly(A)-tail length has been considered critical in post

205 Here we describe poly(A)-tail length profiling by sequencing (PAL-seq) an

206 During egg activation, relative changes in poly(A)-tail length, and thus translational efficiency,

207 When subjected to poly(A) tail-length assays, mitochondrial mRNAs from aff

208 ols germ line cyst development by regulating poly(A) tail lengths of several targets including Cyclin

209 Poly(A) tail lengths were similar for target mRNAs on po

210 RNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding

211 , we profiled translational efficiencies and poly(A)-tail lengths throughout Drosophila oocyte matura

212 Poly(A)-tail lengths were conserved between orthologous

213 nuclear-retained noncoding RNA with a short poly(A) tail-like moiety and a small tRNA-like cytoplasm

214 cay is generally initiated by removal of the poly(A) tail mediated by the Ccr4-Caf1-Not complex.

215 ng, and therefore reduced canonical (cap-and-poly(A)-tail-mediated) translation, remains undiscovered

216 ve levels of A17-PABPN1 and detected shorter poly(A) tails, modest changes in poly(A) signal (PAS) us

217 and the poly(A) tail are required, (ii) the poly(A) tail must be a minimum of 11 to 12 residues in l

218 these results indicate that, in addition to poly(A) tails, Nab2 can also recognize sequence motifs e

219 These results establish that neither the poly(A) tail nor PAB1 is required in yeast for discrimin

220 space between the termination codon and the poly(A) tail nor the binding of steady-state, largely hy

221 higher levels of expression by targeting the poly (A) tails of mRNAs.

222 The length of the poly(A) tail of an mRNA plays an important role in trans

223 promoter is the wild-type 3' CSE followed a poly(A) tail of at least 11 residues.

224 tic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes

225 While the 5' cap modification and the poly(A) tail of eukaryotic mRNA play key roles in regula

226 al protein, many copies of which bind to the poly(A) tail of eukaryotic mRNAs to promote translation

227 GLD-2-GLD-3 acts by extending the short poly(A) tail of germ-line-specific mRNAs, switching them

228 ng a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral

229 roximately 25% of the expressed genes have a poly(A) tail of less than 30 residues in a significant p

230 Proteins bound to the poly(A) tail of mRNA transcripts, called poly(A)-binding

231 PABP is able to bind the poly(A) tail of mRNA, as well as translation initiation

232 d this is accompanied by extension of the 3' poly(A) tail of the AVP mRNA, and the up-regulation of t

233 ic) conditions, eNOS mRNA possessed short 3' poly(A) tails of <25 nt.

234 mitochondrial mRNAs, although the length of poly(A) tails of mitochondrial transcripts were unaffect

235 ing an epitope-tagged protein that binds the poly-A tail of mRNAs (FLAG::PAB-1) from an intestine-spe

236 lar structures may be formed by the cellular poly(A) tails on mRNA.

237 c templates ensures the synthesis of long 3' poly(A) tails on progeny RNA genomes.

238 transferase reactions, leads to synthesis of poly(A) tails on the 3' end of VSV mRNAs that are 10- or

239 polymerase (PAP) catalyzes the synthesis of poly(A) tails on the 3'-end of pre-mRNA.

240 eir 3' untranslated regions (UTRs) have long poly(A) tails; once the RNAs are spliced and transported

241 s the enzyme responsible for the addition of poly(A) tails onto RNA molecules in Escherichia coli.

242 , including mRNAs, for degradation by adding poly(A) tails onto their 3' ends, these data indicate th

243 that recruits poly(A) nuclease(s) to the 3' poly(A) tail-PABP complex.

244 ssenger RNA function is controlled by the 3' poly(A) tail (PAT) and poly(A)-binding protein (PABP).

245 remain untranslated, whereas elongating the poly(A) tail permits protein production.

246 The poly(A) tail protects the mRNA from unregulated degradat

247 largely to those obtained from conventional poly-A tail purification methods, indicating both enumer

248 ssembly in vivo, suggesting new functions of poly(A) tail regulation in RNP dynamics.

249 ession and accelerated decay caused by rapid poly(A) tail removal [3, 5-12].

250 RNA expression posttranscriptionally through poly(A) tail removal.

251 encountered, translation continues into the poly(A) tail, resulting in C-terminal appendage of a pol

252 are uncoupled from transcription and exhibit poly(A) tail rhythms even though the steady-state mRNA l

253 transcribed rhythmically but do not exhibit poly(A) tail rhythms.

254 ondrial ribosome profiling and mitochondrial poly(A)-tail RNA sequencing (MPAT-Seq) assay, we identif

255 Whereas the poly(A) tail serves to provide such a tag at the 3' end

256 NA (mRNA) degradation, which is initiated by poly(A) tail shortening (deadenylation).

257 tent with this, Pum-Brat repression leads to poly(A) tail shortening and mRNA degradation in tissue c

258 This activity was associated with poly(A) tail shortening and regulated by heterogeneous n

259 the decay of SINV RNAs was not initiated by poly(A) tail shortening in either cell line except when

260 Deadenylation, also called poly(A) tail shortening, is the first rate-limiting step

261 Deadenylation, also called poly(A) tail shortening, is the first, rate-limiting ste

262 F)1 in the CCR4-NOT complex function in mRNA poly(A) tail shortening.

263 yadenylation, which indicated that selective poly(A)-tail shortening primarily specifies these change

264 Poly(A) tails stimulate RNA degradation in bacteria, sug

265 slation on transcripts containing or lacking poly(A) tails, suggesting that cleavage of PABP and IRES

266 Premature termination of poly(A) tail synthesis in the presence of cordycepin abr

267 Activation requires shortened/no poly(A)-tail targets; polyadenylated mRNAs are partially

268 Nearly all eukaryotic mRNAs end with a poly(A) tail that is added to their 3' end by the ubiqui

269 re is a >30-fold increase of PAP I-dependent poly(A) tails that are </=10 nt in length coupled with a

270 translationally repressed mRNAs contain long poly(A) tails that are dramatically shortened during the

271 ventional poly(A) polymerase, which produces poly(A) tails that stabilize RNAs.

272 (NOT)," which catalyzes the removal of mRNA poly-(A) tails, the first obligatory step in mRNA decay.

273 day-dependent oscillation for the Fabp7 mRNA poly(A) tail throughout murine brain.

274 t decapping is preceded by shortening of the poly(A) tail to a length that can no longer support tran

275 Polyadenylation is the addition of a poly(A) tail to an RNA molecule.

276 elements are linked to the propensity of the poly(A) tail to engage in double-stranded structures.

277 mRNA polyadenylation, the addition of a poly(A) tail to the 3'-end of pre-mRNA, is a process cri

278 ble miRNA-binding sites by looping the 3'UTR poly(A) tail to the bound miRISC and deadenylase.

279 The addition of the poly(A) tail to the ends of eukaryotic mRNAs is catalyze

280 nal step of mRNA biogenesis, trimming of the poly(A) tail to the length found on newly exported mRNAs

281 The relationship of the poly(A) tail to translational control is intimately rela

282 We have demonstrated that B. halodurans adds poly(A) tails to the 3' ends of RNAs in vivo.

283 ter trimming, amplification primer trimming, poly-A tail trimming, vector screening and low quality r

284 arise given that ribosomal RNA lacks the 3' poly-A tail typically associated with messenger RNA.

285 Third, the poly(A) tail was necessary for maximal PUM repression.

286 Shear-induced lengthening of the eNOS 3' poly(A) tail was the result of increased nuclear polyade

287 polymerase, and the mRNA transcribed, with a poly(A) tail, was efficiently utilized in an in vitro tr

288 where the majority of the PAP I synthesized poly(A) tails were after the Rho-independent transcripti

289 regulated by RNAi in Chlamydomonas, very few poly(A) tails were detected in chloroplasts for the atpB

290 the number or sequence of mitochondrial mRNA poly(A) tails, where unexpectedly we found, in addition

291 Analysis of poly(A) tails, which destabilize chloroplast RNAs, indic

292 EMT/metastasis-related mRNAs to have shorter poly(A) tails while other mRNAs to have longer poly(A) t

293 Orb2 represses translation and removes mRNA poly(A) tails, while the oligomeric form enhances transl

294 turely translated: a transcript with a short poly(A) tail will remain untranslated, whereas elongatin

295 on, we complexed synthetic mRNA containing a poly A tail with PABPs in a stoichiometric manner and st

296 It adds approximately 30-nt poly(A) tails with a rapid, processive burst in which th

297 nstrate that our tool can precisely identify poly(A) tails with near perfect accuracy at the speed re

298 comprised of 2417 nucleotides, excluding the poly(A) tail, with a large open reading frame of 2106 nu

299 onfirm that cordycepin reduces the length of poly(A) tails, with some mRNAs being much more sensitive

300 Mature MALAT1 thus lacks a poly(A) tail yet is expressed at a level higher than man