ライフサイエンスコーパス: poly(A) mRNA

1 ACK1/PKCbeta2 associated with polysome-bound polyA-mRNAs.

2 ncomitant increase in dendritic and synaptic poly(A) mRNA.

3 sequences, we demonstrated the presence of a poly(A) mRNA.

4 tion in the first step of the degradation of poly(A) mRNA.

5 hows little enhancement of expression of non-poly(A) mRNA.

6 plied to primer extension analysis of kidney poly(A) mRNA.

7 proteins, or the cytoplasmic localization of poly(A) mRNA.

8 each of which derepresses translation of non-poly(A) mRNA.

9 nd observing a concomitant decrease in total poly(A)+ mRNA.

10 from poly(A)- mRNA but had little effect on poly(A)+ mRNA.

11 ed nonpoly(A) mRNA with the same kinetics as polyA(+) mRNA.

12 l beta2 subunit were cloned from human heart poly(A)(+) mRNA.

13 RNA is described, separating mouse rRNA from poly(A)(+) mRNA.

14 pap1-1 mutation results in reduced levels of poly(A)(+) mRNAs.

15 inguish between cap(+)/poly(A)(+) and cap(-)/poly(A)(-) mRNAs.

16 Slt2 is required for nuclear poly(A(+)) mRNA accumulation upon heat shock, and thermo

17 Translation of poly(A)- mRNAs after polyadenylation shut-off might resu

18 , we found that sympathetic axons do contain poly(A+) mRNA along their length in a pattern that chang

19 between the polyadenylation machinery, newly poly(A) mRNAs, and factors for transcript export.

20 We show that significant amounts of poly(A)+ mRNAs are oxidized in AD brains.

21 7 mutation derepresses the expression of non-poly(A) mRNA as much as a quadruple ski2 ski3 ski7 ski8

22 nificant percentage of poly(A)-deficient and poly(A)- mRNA associate with smaller polyribosomes compa

23 itro, PKCbeta2 can phosphorylate a subset of polyA-mRNA-associated proteins that are also phosphoryla

24 actions of HuR with beta-actin mRNA and with poly(A)+ mRNA at both native and increased HuR expressio

25 ng the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature.

26 First, we show that both poly(A)(-) and poly(A)(+) mRNA become translationally repressed during

27 ts with poly(A) polymerase and with Npl3p, a poly(A)(+) mRNA binding protein implicated in pre-mRNA p

28 teins, polyA-binding protein 1 and a 130 kDa polyA-mRNA binding protein (KIAA0217).

29 ified Sec61alpha,beta and ribophorin I as ER-poly(A) mRNA-binding proteins, suggesting unexpected rol

30 ength of the 3'UTR increased expression from poly(A)- mRNA but had little effect on poly(A)+ mRNA.

31 the presence of normal amounts of competing poly(A)(+) mRNA, but is normally prevented from doing so

32 hat Ski2p and Slh1p block translation of non-poly(A) mRNA by an effect on Fun12p, possibly by affecti

33 Herein, we provide evidence that poly(A)(+) mRNAs can enter P-bodies in yeast.

34 and defense compounds, northern analysis of poly(A)+ mRNA demonstrates that transcripts encoding CYP

35 cation-dependent (RD) histone genes generate poly(A)- mRNA encoding new histones to package DNA durin

36 molog of the yeast Gle1 involved in the same poly(A) mRNA export pathway as Nup159, also result in se

37 due to a twofold-increased repression of non-poly(A) mRNA expression.

38 cloned by reverse transcription of juice-sac poly(A)+ mRNA, followed by Taq Polymerase-mediated ampli

39 oligo(dt) magnetic bead protocol to harvest poly(A) mRNA from cultured cells in 96-well plates minim

40 In screening amplified poly(A) mRNA from hippocampal dendrites and growth cones

41 AR1 is transcribed as an 800-nucleotide (nt) poly(A)+ mRNA from a promoter lacking a consensus TATA s

42 Northern blot analysis of poly(A)+ mRNA from N. benthamiana and N. tabacum cv. MD6

43 t1 showed a single band of approx. 1.6 kb in poly(A)+ mRNAs from epidermis, limb bud or claw muscle a

44 of single cells; the physical separation of polyA(+) mRNA from genomic DNA using a modified oligo-dT

45 el sequencing of genomic DNA and full-length polyA(+) mRNA from single cells.

46 PolyA+ mRNA from the TGs of each group was reverse trans

47 In some cells, RD H2A.X poly(A)- mRNA generates sufficient histone for depositio

48 ation partially slows elongation, permitting poly(A) mRNA in the ribosome's decoding center to adopt

49 perature and partially restore the levels of poly(A)(+) mRNA in a manner distinct from the cytoplasmi

50 h Pab1p does not diminish the preference for poly(A)(+) mRNA in vivo, indicating another role for pol

51 Because of the absence of poly(A)+ mRNA in prokaryotic organisms, studies of diffe

52 used accumulation of active spliceosomes and poly(A)+ mRNAs in unique splicing clusters located at th

53 protein is localized at the nuclear rim, and poly(A)-mRNA in situ hybridization shows that mRNA expor

54 nslation, allowing better translation of non-poly(A) mRNA, including the L-A virus mRNA which lacks p

55 We now demonstrate that Ram-1 poly(A)+ mRNA increases significantly following culture

56 Northern analyses of poly(A) mRNA indicated two major species of about 8 and

57 ay facilitate the localization of associated poly(A) mRNAs into axons.

58 o not bind sperm, but injection of total egg poly(A)+ mRNA into immature oocytes confers sperm bindin

59 late that the derepressed translation of non-poly(A) mRNAs is due to abnormal (but full-size) 60S sub

60 use (P:D ratio) of the resulting cytoplasmic poly(A)+ mRNA is a measure of poly(A) site strength.

61 ctivated PKCbeta2 to mRNP complexes bound to polyA-mRNAs is involved in activity-triggered control of

62 d histone gene, generating both poly(A)+ and poly(A)- mRNA isoforms.

63 a specific reduction of both HuD protein and poly(A) mRNA levels in the axonal compartment.

64 mance: specificity of 1:250,000 in mammalian poly(A(+)) mRNA; limit of detection 0.13 pM; dynamic ran

65 We suggest that rapid degradation of non-poly(A) mRNAs may be primarily responsible for the shape

66 In this study, we developed an engineered poly(A) mRNA mimic, AAAAAAAAAAAA, named A12, with 2'-O-m

67 Poly(A) mRNA-mRNP complexes were purified from a postmit

68 : the intracellular distribution of cellular poly(A)(+) mRNA, nuclear proteins, and, most important,

69 ski8 mutations enhance the expression of the poly(A)(-) mRNAs of yeast RNA viruses.

70 nter P-bodies, and an mRNP complex including poly(A)(+) mRNA, Pab1p, eIF4E, and eIF4G2 may represent

71 romoting translation from poly(A)(+) but not poly(A)(-) mRNAs, particularly for mRNAs containing seco

72 se the accumulation of the secretory form of poly(A)(+) mRNA relative to the membrane form and regula

73 In contrast, cells making predominantly poly(A)+ mRNA require this isoform for de novo H2A.X syn

74 much higher concentrations of yeast tRNA or poly(A)mRNA, respectively, 33- and 60-fold greater than

75 ors generated from hippocampal and forebrain poly(A)+ mRNA revealed greater sensitivity to 2,3-benzod

76 Northern analysis of poly(A+) mRNAs reveals two differently sized rnp-4f mRNA

77 ms of a specific mRNA showed relatively more poly(A)- mRNA sedimenting with 20-60 S complexes than do

78 ing, specifically reduces the translation of poly(A)(+) mRNA, suggesting that poly(A) may have a role

79 f p53 and to the inhibition of total RNA and poly(A) mRNA synthesis.

80 loss-of-function mutant, we showed that XRN4 poly(A(+)) mRNA targets are largely subject to cotransla

81 oach we have isolated a single cDNA from egg poly(A)+ mRNA that can induce sperm binding in immature

82 pap1-1 mutation results in the synthesis of poly(A)- mRNAs that initiate translation with surprising

83 redominantly associated with polysome-bound, polyA-mRNAs that are being actively translated.

84 For poly(A)+ mRNA, the translational efficiency and mRNA hal

85 ryotes, the nucleocytoplasmic export of bulk poly(A)(+)-mRNAs through the nuclear pore complex is med

86 on-independent (RI) histone genes synthesise poly(A)+ mRNA throughout the cell cycle, translated into

87 Hybridization of poly(A)(+) mRNA to DNA microarrays containing 96.4% of y

88 of HYPB/Setd2, like Iws1, induced bulk HeLa poly(A)+ mRNAs to accumulate in the nucleus.

89 factors to mRNA, caused by the inability of poly(A)- mRNAs to accumulate to normal levels.

90 lse-labeling experiments indicate that total poly(A)(+) mRNA transcription was not significantly redu

91 Poly(A) mRNA was present in the nucleus and throughout t

92 tubers, we developed a methodology in which poly(A)+ mRNA was amplified from immunohistochemically l

93 ocesses, reverse transcription-PCR with PC-3 poly(A)+ mRNA was performed by using degenerate oligonuc

94 Poly(A)+mRNA was present in the axon tips, and was more

95 The total population of polyadenylated [poly(A)] mRNA was localized in hippocampus using a bioti

96 contrast, highly toxic 36R in the context of poly(A)(+) mRNA were exported to the cytoplasm, where DP

97 Tissue-specific libraries from poly(A)(+) mRNA were prepared by priming first and secon

98 sed the expression of nonpolyadenylated [non-poly(A)] mRNAs, whether capped or uncapped, thus explain

99 The labeled granules colocalized with poly(A+) mRNA, with the 60S ribosomal subunit, and with