ライフサイエンスコーパス: poly(A) mRNA

1 ACK1/PKCbeta2 associated with polysome-bound polyA-mRNAs.

2 sequences, we demonstrated the presence of a poly(A) mRNA.

3 tion in the first step of the degradation of poly(A) mRNA.

4 hows little enhancement of expression of non-poly(A) mRNA.

5 plied to primer extension analysis of kidney poly(A) mRNA.

6 proteins, or the cytoplasmic localization of poly(A) mRNA.

7 each of which derepresses translation of non-poly(A) mRNA.

8 ncomitant increase in dendritic and synaptic poly(A) mRNA.

9 nd observing a concomitant decrease in total poly(A)+ mRNA.

10 from poly(A)- mRNA but had little effect on poly(A)+ mRNA.

11 ed nonpoly(A) mRNA with the same kinetics as polyA(+) mRNA.

12 l beta2 subunit were cloned from human heart poly(A)(+) mRNA.

13 RNA is described, separating mouse rRNA from poly(A)(+) mRNA.

14 pap1-1 mutation results in reduced levels of poly(A)(+) mRNAs.

15 inguish between cap(+)/poly(A)(+) and cap(-)/poly(A)(-) mRNAs.

16 Slt2 is required for nuclear poly(A(+)) mRNA accumulation upon heat shock, and thermo

17 Translation of poly(A)- mRNAs after polyadenylation shut-off might resu

18 , we found that sympathetic axons do contain poly(A+) mRNA along their length in a pattern that chang

19 between the polyadenylation machinery, newly poly(A) mRNAs, and factors for transcript export.

20 We show that significant amounts of poly(A)+ mRNAs are oxidized in AD brains.

21 7 mutation derepresses the expression of non-poly(A) mRNA as much as a quadruple ski2 ski3 ski7 ski8

22 nificant percentage of poly(A)-deficient and poly(A)- mRNA associate with smaller polyribosomes compa

23 itro, PKCbeta2 can phosphorylate a subset of polyA-mRNA-associated proteins that are also phosphoryla

24 actions of HuR with beta-actin mRNA and with poly(A)+ mRNA at both native and increased HuR expressio

25 ng the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature.

26 First, we show that both poly(A)(-) and poly(A)(+) mRNA become translationally repressed during

27 ts with poly(A) polymerase and with Npl3p, a poly(A)(+) mRNA binding protein implicated in pre-mRNA p

28 teins, polyA-binding protein 1 and a 130 kDa polyA-mRNA binding protein (KIAA0217).

29 ified Sec61alpha,beta and ribophorin I as ER-poly(A) mRNA-binding proteins, suggesting unexpected rol

30 ength of the 3'UTR increased expression from poly(A)- mRNA but had little effect on poly(A)+ mRNA.

31 the presence of normal amounts of competing poly(A)(+) mRNA, but is normally prevented from doing so

32 hat Ski2p and Slh1p block translation of non-poly(A) mRNA by an effect on Fun12p, possibly by affecti

33 Herein, we provide evidence that poly(A)(+) mRNAs can enter P-bodies in yeast.

34 and defense compounds, northern analysis of poly(A)+ mRNA demonstrates that transcripts encoding CYP

35 molog of the yeast Gle1 involved in the same poly(A) mRNA export pathway as Nup159, also result in se

36 due to a twofold-increased repression of non-poly(A) mRNA expression.

37 cloned by reverse transcription of juice-sac poly(A)+ mRNA, followed by Taq Polymerase-mediated ampli

38 oligo(dt) magnetic bead protocol to harvest poly(A) mRNA from cultured cells in 96-well plates minim

39 In screening amplified poly(A) mRNA from hippocampal dendrites and growth cones

40 AR1 is transcribed as an 800-nucleotide (nt) poly(A)+ mRNA from a promoter lacking a consensus TATA s

41 Northern blot analysis of poly(A)+ mRNA from N. benthamiana and N. tabacum cv. MD6

42 t1 showed a single band of approx. 1.6 kb in poly(A)+ mRNAs from epidermis, limb bud or claw muscle a

43 of single cells; the physical separation of polyA(+) mRNA from genomic DNA using a modified oligo-dT

44 el sequencing of genomic DNA and full-length polyA(+) mRNA from single cells.

45 PolyA+ mRNA from the TGs of each group was reverse trans

46 perature and partially restore the levels of poly(A)(+) mRNA in a manner distinct from the cytoplasmi

47 h Pab1p does not diminish the preference for poly(A)(+) mRNA in vivo, indicating another role for pol

48 Because of the absence of poly(A)+ mRNA in prokaryotic organisms, studies of diffe

49 protein is localized at the nuclear rim, and poly(A)-mRNA in situ hybridization shows that mRNA expor

50 nslation, allowing better translation of non-poly(A) mRNA, including the L-A virus mRNA which lacks p

51 We now demonstrate that Ram-1 poly(A)+ mRNA increases significantly following culture

52 Northern analyses of poly(A) mRNA indicated two major species of about 8 and

53 ay facilitate the localization of associated poly(A) mRNAs into axons.

54 o not bind sperm, but injection of total egg poly(A)+ mRNA into immature oocytes confers sperm bindin

55 late that the derepressed translation of non-poly(A) mRNAs is due to abnormal (but full-size) 60S sub

56 use (P:D ratio) of the resulting cytoplasmic poly(A)+ mRNA is a measure of poly(A) site strength.

57 ctivated PKCbeta2 to mRNP complexes bound to polyA-mRNAs is involved in activity-triggered control of

58 a specific reduction of both HuD protein and poly(A) mRNA levels in the axonal compartment.

59 mance: specificity of 1:250,000 in mammalian poly(A(+)) mRNA; limit of detection 0.13 pM; dynamic ran

60 Poly(A) mRNA-mRNP complexes were purified from a postmit

61 : the intracellular distribution of cellular poly(A)(+) mRNA, nuclear proteins, and, most important,

62 ski8 mutations enhance the expression of the poly(A)(-) mRNAs of yeast RNA viruses.

63 nter P-bodies, and an mRNP complex including poly(A)(+) mRNA, Pab1p, eIF4E, and eIF4G2 may represent

64 romoting translation from poly(A)(+) but not poly(A)(-) mRNAs, particularly for mRNAs containing seco

65 se the accumulation of the secretory form of poly(A)(+) mRNA relative to the membrane form and regula

66 much higher concentrations of yeast tRNA or poly(A)mRNA, respectively, 33- and 60-fold greater than

67 ors generated from hippocampal and forebrain poly(A)+ mRNA revealed greater sensitivity to 2,3-benzod

68 Northern analysis of poly(A+) mRNAs reveals two differently sized rnp-4f mRNA

69 ms of a specific mRNA showed relatively more poly(A)- mRNA sedimenting with 20-60 S complexes than do

70 ing, specifically reduces the translation of poly(A)(+) mRNA, suggesting that poly(A) may have a role

71 f p53 and to the inhibition of total RNA and poly(A) mRNA synthesis.

72 oach we have isolated a single cDNA from egg poly(A)+ mRNA that can induce sperm binding in immature

73 pap1-1 mutation results in the synthesis of poly(A)- mRNAs that initiate translation with surprising

74 redominantly associated with polysome-bound, polyA-mRNAs that are being actively translated.

75 For poly(A)+ mRNA, the translational efficiency and mRNA hal

76 Hybridization of poly(A)(+) mRNA to DNA microarrays containing 96.4% of y

77 of HYPB/Setd2, like Iws1, induced bulk HeLa poly(A)+ mRNAs to accumulate in the nucleus.

78 factors to mRNA, caused by the inability of poly(A)- mRNAs to accumulate to normal levels.

79 lse-labeling experiments indicate that total poly(A)(+) mRNA transcription was not significantly redu

80 Poly(A) mRNA was present in the nucleus and throughout t

81 tubers, we developed a methodology in which poly(A)+ mRNA was amplified from immunohistochemically l

82 ocesses, reverse transcription-PCR with PC-3 poly(A)+ mRNA was performed by using degenerate oligonuc

83 Poly(A)+mRNA was present in the axon tips, and was more

84 The total population of polyadenylated [poly(A)] mRNA was localized in hippocampus using a bioti

85 contrast, highly toxic 36R in the context of poly(A)(+) mRNA were exported to the cytoplasm, where DP

86 Tissue-specific libraries from poly(A)(+) mRNA were prepared by priming first and secon

87 sed the expression of nonpolyadenylated [non-poly(A)] mRNAs, whether capped or uncapped, thus explain

88 The labeled granules colocalized with poly(A+) mRNA, with the 60S ribosomal subunit, and with