ライフサイエンスコーパス: poly(A) tail

1 hesize involves stalling of ribosomes on the polyA tail.

2 e end in a conserved stem-loop rather than a polyA tail.

3 MKIIalpha protein and mRNA by shortening its poly(A) tail.

4 nd cytoplasmic lengthening of the neg-1 mRNA poly(A) tail.

5 n the renal outer medulla appeared to lack a poly(A) tail.

6 precursor, cleaves at that site, and adds a poly(A) tail.

7 leolytic cleavage followed by synthesis of a poly(A) tail.

8 codons or translational readthrough into the poly(A) tail.

9 tly translated in vivo despite the lack of a poly(A) tail.

10 nding that PUMs repress an mRNA that lacks a poly(A) tail.

11 e U-rich internal loop of the ENE and the 3'-poly(A) tail.

12 cription or translation or the status of the poly(A) tail.

13 capping by the addition of a CUCU tag to the poly(A) tail.

14 that hybridizes to and protects the PAN RNA poly(A) tail.

15 ling in ribosomes on translation through the poly(A) tail.

16 pended upon the mRNA's 5' cap but not its 3' poly(A) tail.

17 s end in a conserved stem-loop rather than a poly(A) tail.

18 t recognize the cis-elements and produce the poly(A) tail.

19 ndent on the length of both the mRNA and the poly(A) tail.

20 nterplay between the 5' m(7)G cap and the 3' poly(A) tail.

21 X(L) mRNA was dependent upon the presence of poly(A) tail.

22 PEB, but after polyadenylation, it binds the poly(A) tail.

23 g in the stem-loop but not a reporter with a poly(A) tail.

24 ot distinguish RNAs with or without a cap or poly(A) tail.

25 mRNAs in that they possess a 5' cap and a 3' poly(A) tail.

26 POP2 levels induce a lengthening of tim mRNA poly(A) tail.

27 aves pre-mRNAs at a specific site and adds a poly(A) tail.

28 ed with maintenance and/or extension of long poly(A) tails.

29 le for mixed tailing and protection of viral poly(A) tails.

30 4 with mRNAs containing critically shortened poly(A) tails.

31 of single-stranded, positive-sense RNAs with poly(A) tails.

32 ust circadian rhythms in the length of their poly(A) tails.

33 these RNAs while stabilizing mRNAs with long poly(A) tails.

34 iduals were shown to have severely truncated poly(A) tails.

35 is known to trim hTR precursors by removing poly(A) tails.

36 uncated AU-rich mRNAs lacking the 3' UTR and poly(A) tails.

37 nto structures previously thought to be long poly(A) tails.

38 A and mRNA; and the Distal Site, which binds poly(A) tails.

39 o fractions dependent on the length of their poly(A) tails.

40 -seq that directly sequences the full-length poly(A) tails.

41 ter transcripts lack splicing signatures and poly(A) tails.

42 pore complexes and the length of transcript poly(A) tails.

43 ly(A) tails while other mRNAs to have longer poly(A) tails.

44 rse transcription polymerase chain reaction, polyA tail, 3' rapid amplification of complementary DNA

45 earrangements (21%), 5' truncation (74%) and polyA tails (88%).

46 bundance of the mRNA subpopulation with long poly(A) tails (a rough proxy for mRNA translatability).

47 processes, it is important to identify these poly(A) tails accurately in transcriptome sequencing dat

48 In eukaryotes, the poly(A) tail added at the 3' end of an mRNA precursor is

49 ate annotation of positional information for polyA tails added posttranscriptionally.

50 of a targeted gene, subsequently inhibiting poly(A) tail addition and leading to degradation of that

51 on in most eukaryotes, involves cleavage and poly(A) tail addition at the 3' end of mRNAs at the poly

52 of U1 snRNP to the target pre-mRNA inhibits poly(A)-tail addition, causing degradation of that RNA s

53 ansport and translation, suggesting that the poly(A)-tail also provides a basis for eukaryotes to eff

54 cells accomplish similar decoupling through poly(A) tail alterations to ensure that dormant transcri

55 ells, the oocyte transcriptome has a shorter poly(A) tail and a higher relative proportion of termina

56 down cells, p53 mRNA has an abnormally short poly(A) tail and a reduced translational efficiency, res

57 on of an mRNA is strongly impacted by its 3' poly(A) tail and associated poly(A)-binding proteins (PA

58 nteraction of topoisomerase IIalpha with the poly(A) tail and G/U-rich 3'-untranslated region (3'-UTR

59 nome of TV contains 6,714 nucleotides plus a poly(A) tail and is organized into three open reading fr

60 FLuc production rate that was dependent on a poly(A) tail and poly(A)-binding protein, but was indepe

61 tramolecular RNA clamp, sequestering the PAN poly(A) tail and preventing the initiation of RNA decay.

62 s between polysomes and P-bodies and how the poly(A) tail and the associated poly(A) binding protein

63 proximity of the termination codon to the 3' poly(A) tail and the poly(A) RNA-binding protein, PAB1,

64 nctionally replaced interactions between the poly(A) tail and the poly(A)-binding protein (PABP) to a

65 As: efficiently translated mRNAs have longer poly(A) tails and are shorter, more stable, and more eff

66 can therefore interact with mRNAs via their poly(A) tails and caps, as well as through sequence-spec

67 cells into two fractions with short and long poly(A) tails and compared them by microarray analysis.

68 ocol that queries the junctions of 3'UTR and poly(A) tails and confidently maps the poly(A) tags to t

69 form enhances translation and elongates the poly(A) tails and imparts its translational state to the

70 eotides to capture the RNAs at their natural poly(A) tails and initiate sequencing by synthesis.

71 either GLD-2 or RNP-8 resulted in shortened poly(A) tails and lowered abundance of four target mRNAs

72 length, while GC-rich Alus only in their 3' poly(A) tails and middle A-stretches, with differences d

73 ource tool for finding the precise border of poly(A) tails and other homopolymers in raw mRNA sequenc

74 n that highly expressed genes can have short poly(A) tails and the elucidation of the seemingly contr

75 levels of CDK11 increased the length of HIV poly(A) tails and the stability of mature viral transcri

76 ort a new technique to analyse the length of poly(A) tails and to separate a mixed population of mRNA

77 majority of these products contained intact poly(A) tails and were bound by the poly(A) binding prot

78 n RNA during cDNA synthesis by aiming at the poly(A)+-tail and (2) introduced a pre-amplification of

79 ion, independent of 7-methylguanylate cap or poly(A) tail, and prompts mRNA redistribution to silenci

80 inding protein N1 (PABPN1) and PABPC1 at the poly(A) tail, and, provided biogenesis involves pre-mRNA

81 nscripts with a high frequency of very short poly(A) tails, and a loss of 3' oligo-uridylation.

82 L1/MALAT RNAs accumulate in cells, lack poly(A) tails, and are translated; however, they cannot

83 a mice have mild histologic defects, shorter poly(A) tails, and evidence of mitochondrial damage.

84 peroxidase (HRP)-biotin/streptavidin to the poly(A) tails, and the oxidation reaction of tetramethyl

85 ith much less starting material, the lack of poly(A)-tails, and the fact that the messages can be pol

86 Poly(A) tails are 3' modifications of eukaryotic mRNAs t

87 mRNA poly(A) tails are important for mRNA stability and trans

88 Therefore, we propose that mRNA poly(A) tails are important in regulating protein synthe

89 Following the resumption of meiosis, the poly(A) tails are lengthened and translation ensues.

90 Poly(A) tails are non-templated additions of adenosines

91 new insights into how transcripts that lack poly(A) tails are stabilized and regulated and suggest t

92 Here we identify a terminal poly(A) tail as being important for a subset of intron e

93 rity of both forms requires the mRNA cap and poly(A) tail, as well as eIF4E, eIF4G, Pab1 and eIF3, an

94 paving the way for a better understanding of poly(A) tail-associated regulation of gene expression.

95 transcripts have a 5' monophosphate, lack a poly(A) tail at the 3' end, and contain no introns; thes

96 ssenger RNA (mRNA) does not terminate with a poly(A) tail at the 3'-end.

97 timulated hypothalamus shortens the AVP mRNA poly(A) tail at the same time as reducing transcript abu

98 ions of 3'-untranslated region (UTR) and the poly(A) tails at the transcriptome level, a comprehensiv

99 We uncover a poly(A) tail-based regulatory mechanism that dynamically

100 tic initiation factor 4E (eIF4E), eIF4G, and poly(A) tail-binding protein (PABP) that circularizes mR

101 cal structure that could not only shield the poly(A) tail but also serve as a scaffold for the assemb

102 ctor 4E (eIF4E) at the cap and PABPC1 at the poly(A) tail, but that lacks detectable EJCs and PABPN1.

103 ranscribed by Pol II and acquire 5' caps and poly(A) tails, but only mRNAs are translated into protei

104 Coating of the poly(A) tail by mtPABP1, however, did not lead to transc

105 obe DNAs, extension reaction of polyadenine (poly(A)) tails by poly(A) polymerase, binding of a terna

106 ve AAA codons, sensing of prematurely placed polyA tails by a specialized RNA-binding protein is a no

107 We demonstrate that mRNA in yeast lacking a poly(A) tail can be destabilized by introduction of a pr

108 ion of mRNAs, consistent with alterations in poly(A) tail chain termination.

109 end in a stem-loop structure instead of the poly(A) tail characteristic of all other mature mRNAs.

110 Many plant viruses without 5' caps or 3' poly(A) tails contain 3' proximal, cap-independent trans

111 More than 10% of poly(A) tails contain at least one guanosine (G); among

112 w dwarf virus RNA, lacking a 5' cap and a 3' poly(A) tail, contains a cap-independent translation ele

113 ion in myoblasts led to a shortening of mRNA poly(A) tails, demonstrating the cellular function of PA

114 ing proteins directly recruit PABP, in a non-poly(A) tail-dependent manner, to stimulate the small su

115 supports export and translation as well as a poly(A) tail does.

116 eadenylases are best known for degrading the poly(A) tail during mRNA decay.

117 tion motif of HuD, the domain that binds the poly(A) tail, eliminated the branch-specific expression

118 The WISP protein is required for poly(A) tail elongation of bicoid, Toll, and torso mRNAs

119 specific genes through deadenylation of mRNA poly(A) tails, enabling positive selection.

120 rithms struggle to accurately identify these poly(A) tail end-points.

121 Poly(A) tails enhance the stability and translation of m

122 ally, we show that neither a 5' cap nor a 3' poly(A) tail enhances Sec incorporation.

123 While greater distances with the poly(A) tail exacerbate dependency on PABP for deadenyla

124 to 3' stem-loop structures not involving the poly(A) tail exhibit even longer half-lives.

125 rase WISPY is responsible for stage-specific poly(A) tail extension in the female germline.

126 ware of focus exclusively on the trimming of poly(A) tails, failing to provide the detailed informati

127 in part through the removal of PAB1 from the poly(A) tail following its self-association into multime

128 nterestingly, REF/Aly appears to protect the poly(A) tail from deadenylation, and REF/Aly-stabilized

129 engths have impeded greater understanding of poly(A)-tail function.

130 In 3' RACE, the poly(A) tail functions as a non-specific tag at the 3' e

131 In eukaryotes, poly(A) tails generally stabilize mature mRNAs, whereas

132 se R, but can be efficiently degraded once a poly(A) tail has been added to their ends.

133 w dwarf virus mRNA, which lacks both cap and poly(A) tail, has a translation element (3'-BTE) in its

134 polyadenylation or retain a reasonably long poly(A) tail if they are to return to the translating po

135 h the ENE's ability to (i) interact with the polyA tail, (ii) inhibit deadenylation in vitro, and (ii

136 n vivo, arguing against a major role for the poly(A) tail in microRNA-mediated silencing.

137 n may be relevant, as the positioning of the poly(A) tail in mRNAs influences the length of the 3'-un

138 earch for the enzymes responsible for adding poly(A) tails in Chlamydomonas and Arabidopsis organelle

139 dentified 213 transcripts that have extended poly(A) tails in Noc KO liver.

140 D(pol) that resulted in shorter or longer 3' poly(A) tails in virion RNA.

141 ovl4, which contained 1794 bp (excluding the polyA tail), including 909 bp of coding region that enco

142 the first example of a cellular IRES that is poly(A) tail-independent.

143 t is a polyU sequence that can interact with poly(A) tails, inhibit the association of poly(A)-bindin

144 e eukaryotic initiation factor (eIF) 4G/PABP/poly(A) tail interaction is achieved instead through the

145 These guanosines frequently divide poly(A) tails into interspersed A-tracts and therefore c

146 A 3' poly(A) tail is a common feature of picornavirus RNA gen

147 e primers are removed by exonuclease I and a poly(A) tail is added to the 3' end of the first-strand

148 The foreshortened poly(A) tail is maintained by poly(A) ribonuclease, whic

149 sequence motifs that signal the addition of poly(A) tails is essential to improved genome annotation

150 One challenge in studying poly(A) tails is that they are difficult to sequence and

151 s tend to possess shorter poly(A)-tails, the poly(A)-tail is not required for the codon-mediated mRNA

152 s are polyadenylated in the nucleus, and the poly(A)-tail is required for efficient mRNA export and t

153 cDNA synthesis specifically toward the 3UTR/poly(A) tail junction of cellular RNA.

154 The alterations in poly(A) tail length accompanying elevated PDE12 expressi

155 In summary, we show that poly(A) tail length and 3' terminal uridylation have ess

156 oly(A) tail length and expression level, and poly(A) tail length and 3'-UTR length.

157 r the rhythmic mRNAs by their peak phases in poly(A) tail length and abundance of the long-tailed sub

158 of previously reported correlations between poly(A) tail length and expression level, and poly(A) ta

159 ar PAPS isoforms control de novo synthesized poly(A) tail length and hence expression of specific sub

160 strongest contributor to the rhythmicity in poly(A) tail length and the rhythmicity in the abundance

161 a function for Hnrnpa1 in the regulation of poly(A) tail length and translation of maternal mRNAs th

162 In fact, changes in poly(A) tail length are not sufficient to account for PN

163 n of pgc is concurrent with extension of its poly(A) tail length but appears largely independent of t

164 NA show growth defects as well as defects in poly(A) tail length but do not accumulate poly(A) RNA in

165 onsequences of altered pre-mRNA splicing and poly(A) tail length control.

166 a suggest that NPM1 has an important role in poly(A) tail length determination and may help network 3

167 st, highly reproducible and minor changes in poly(A) tail length distribution are easily detected.

168 Therefore, even though the poly(A) tail length dynamics seen between genotypes may

169 assays have enabled a more detailed look at poly(A) tail length genome-wide throughout many developm

170 cial for gene expression and perturbation of poly(A) tail length has been linked to a human neurodege

171 help of these advances, our understanding of poly(A) tail length has evolved over the past 5 years wi

172 etic data indicate that dNab2 restricts bulk poly(A) tail length in vivo, suggesting that this functi

173 on in the heart, where controlled changes in poly(A) tail length influence mRNA translation.

174 mportantly, we found that the rhythmicity in poly(A) tail length is closely correlated with rhythmic

175 Poly(A) tail length is emerging as an important marker o

176 also needed during oogenesis to regulate the poly(A) tail length of dmos during oocyte maturation and

177 ial action of RNA binding proteins modulates poly(A) tail length of maternal mRNAs, leading to asymme

178 A)denylome" analysis, a method that measures poly(A) tail length of transcripts in a global manner, a

179 se transcripts do not exhibit rhythmicity in poly(A) tail length or steady-state mRNA level, despite

180 basis for understanding its function in both poly(A) tail length regulation and in the compaction of

181 ly(A) RNA binding protein, Nab2, facilitates poly(A) tail length regulation together with targeting t

182 mRNP organization and compaction as well as poly(A) tail length regulation.

183 This poly(A) tail length restriction is controlled by Mtr4p.

184 d translation, and enzymes that regulate the poly(A) tail length significantly impact protein profile

185 me, consistent with previous reports linking poly(A) tail length with nuclear RNA surveillance.

186 f genes were found to exhibit rhythmicity in poly(A) tail length, and the poly(A) rhythms are strongl

187 We describe strategies for assessing 3' poly(A) tail length, base modifications and transcript h

188 Depletion of GLD4 not only reduced GLUT1 poly(A) tail length, but also GLUT1 protein.

189 define the function of PABPN1 in control of poly(A) tail length, little is known about the role of P

190 r-expression enhances AVP mRNA abundance and poly(A) tail length.

191 r PNPase nor RNase II has any effect on tRNA poly(A) tail length.

192 ing to RNA is critical for proper control of poly(A) tail length.

193 nal mRNAs is regulated by dynamic changes in poly(A) tail length.

194 that modulates both mRNA nuclear export and poly(A) tail length.

195 acterization of ZC3H14 as a regulator of RNA poly(A) tail length.

196 es, splicing events, poly(A) site choice and poly(A) tail length.

197 Furthermore, although poly(A)-tail length has been considered critical in post

198 Here we describe poly(A)-tail length profiling by sequencing (PAL-seq) an

199 During egg activation, relative changes in poly(A)-tail length, and thus translational efficiency,

200 When subjected to poly(A) tail-length assays, mitochondrial mRNAs from aff

201 We also determined that poly(A) tail lengths of transcripts vary across developm

202 Poly(A) tail lengths were similar for target mRNAs on po

203 RNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding

204 miRNAs have almost no effect on steady-state poly(A)-tail lengths of their targets in mouse fibroblas

205 , we profiled translational efficiencies and poly(A)-tail lengths throughout Drosophila oocyte matura

206 Poly(A)-tail lengths were conserved between orthologous

207 cleus with diverse intragenic and intergenic poly(A)-tail lengths.

208 nuclear-retained noncoding RNA with a short poly(A) tail-like moiety and a small tRNA-like cytoplasm

209 cay is generally initiated by removal of the poly(A) tail mediated by the Ccr4-Caf1-Not complex.

210 ng, and therefore reduced canonical (cap-and-poly(A)-tail-mediated) translation, remains undiscovered

211 ve levels of A17-PABPN1 and detected shorter poly(A) tails, modest changes in poly(A) signal (PAS) us

212 A)-binding proteins (PABs) uniformly bind to poly(A)-tailed mRNAs, regulating their stability and tra

213 cytoplasm they contribute to translation of poly(A)-tailed mRNAs.

214 these results indicate that, in addition to poly(A) tails, Nab2 can also recognize sequence motifs e

215 These results establish that neither the poly(A) tail nor PAB1 is required in yeast for discrimin

216 space between the termination codon and the poly(A) tail nor the binding of steady-state, largely hy

217 The expansion, which occurs in the poly(A) tail of an AluSx3 element and differs in both si

218 tic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes

219 While the 5' cap modification and the poly(A) tail of eukaryotic mRNA play key roles in regula

220 al protein, many copies of which bind to the poly(A) tail of eukaryotic mRNAs to promote translation

221 GLD-2-GLD-3 acts by extending the short poly(A) tail of germ-line-specific mRNAs, switching them

222 ng a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral

223 roximately 25% of the expressed genes have a poly(A) tail of less than 30 residues in a significant p

224 The 3' poly(A) tail of messenger RNA is fundamental to regulati

225 Proteins bound to the poly(A) tail of mRNA transcripts, called poly(A)-binding

226 PABP is able to bind the poly(A) tail of mRNA, as well as translation initiation

227 d this is accompanied by extension of the 3' poly(A) tail of the AVP mRNA, and the up-regulation of t

228 well as synthesize 5' methylated cap and 3' poly(A) tail of the transcribed viral mRNAs.

229 mitochondrial mRNAs, although the length of poly(A) tails of mitochondrial transcripts were unaffect

230 synthesis from RNA templates, followed by 3' polyA tailing of the single-stranded cDNA products and d

231 lar structures may be formed by the cellular poly(A) tails on mRNA.

232 c templates ensures the synthesis of long 3' poly(A) tails on progeny RNA genomes.

233 transferase reactions, leads to synthesis of poly(A) tails on the 3' end of VSV mRNAs that are 10- or

234 polymerase (PAP) catalyzes the synthesis of poly(A) tails on the 3'-end of pre-mRNA.

235 eir 3' untranslated regions (UTRs) have long poly(A) tails; once the RNAs are spliced and transported

236 s the enzyme responsible for the addition of poly(A) tails onto RNA molecules in Escherichia coli.

237 , including mRNAs, for degradation by adding poly(A) tails onto their 3' ends, these data indicate th

238 The longest NQO1 transcript has increased poly(A) tail (PA-tail) length that accounts for the diff

239 mination in vitro However, after binding the poly(A) tail, PABP became insensitive to suppression by

240 ssenger RNA function is controlled by the 3' poly(A) tail (PAT) and poly(A)-binding protein (PABP).

241 LARP4 was shown to promote poly(A) tail (PAT) lengthening and stabilization of indi

242 remain untranslated, whereas elongating the poly(A) tail permits protein production.

243 In the cytoplasm, poly(A) tails play pivotal roles in the translation and

244 The poly(A) tail protects the mRNA from unregulated degradat

245 e show that KPAF5 stabilizes KPAF4 to enable poly(A) tail recognition, which likely leads to mRNA sta

246 ssembly in vivo, suggesting new functions of poly(A) tail regulation in RNP dynamics.

247 ession and accelerated decay caused by rapid poly(A) tail removal [3, 5-12].

248 a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate

249 RNA expression posttranscriptionally through poly(A) tail removal.

250 encountered, translation continues into the poly(A) tail, resulting in C-terminal appendage of a pol

251 are uncoupled from transcription and exhibit poly(A) tail rhythms even though the steady-state mRNA l

252 transcribed rhythmically but do not exhibit poly(A) tail rhythms.

253 ondrial ribosome profiling and mitochondrial poly(A)-tail RNA sequencing (MPAT-Seq) assay, we identif

254 NA-binding proteome (RBPome) associated with polyA-tailed RNA species in murine ciliated epithelial c

255 smsDGE involves a reverse-transcription and polyA-tailing sample preparation procedure followed by s

256 Whereas the poly(A) tail serves to provide such a tag at the 3' end

257 NA (mRNA) degradation, which is initiated by poly(A) tail shortening (deadenylation).

258 tent with this, Pum-Brat repression leads to poly(A) tail shortening and mRNA degradation in tissue c

259 This activity was associated with poly(A) tail shortening and regulated by heterogeneous n

260 the decay of SINV RNAs was not initiated by poly(A) tail shortening in either cell line except when

261 Poly(A) tail shortening, also termed deadenylation, is t

262 Deadenylation, also called poly(A) tail shortening, is the first rate-limiting step

263 Deadenylation, also called poly(A) tail shortening, is the first, rate-limiting ste

264 F)1 in the CCR4-NOT complex function in mRNA poly(A) tail shortening.

265 yadenylation, which indicated that selective poly(A)-tail shortening primarily specifies these change

266 ncation of its 3' UTR, including loss of its polyA tail, stabilized Bip1 mRNA, resulting in increased

267 slation on transcripts containing or lacking poly(A) tails, suggesting that cleavage of PABP and IRES

268 Premature termination of poly(A) tail synthesis in the presence of cordycepin abr

269 Activation requires shortened/no poly(A)-tail targets; polyadenylated mRNAs are partially

270 Shortening of the poly(A) tail, termed deadenylation, reduces transcript s

271 termined that eIF4A2 bound mRNAs have longer poly(A) tails than DDX6 bound mRNAs.

272 Nearly all eukaryotic mRNAs end with a poly(A) tail that is added to their 3' end by the ubiqui

273 re is a >30-fold increase of PAP I-dependent poly(A) tails that are </=10 nt in length coupled with a

274 translationally repressed mRNAs contain long poly(A) tails that are dramatically shortened during the

275 ventional poly(A) polymerase, which produces poly(A) tails that stabilize RNAs.

276 destabilizing codons tend to possess shorter poly(A)-tails, the poly(A)-tail is not required for the

277 day-dependent oscillation for the Fabp7 mRNA poly(A) tail throughout murine brain.

278 t decapping is preceded by shortening of the poly(A) tail to a length that can no longer support tran

279 Polyadenylation is the addition of a poly(A) tail to an RNA molecule.

280 elements are linked to the propensity of the poly(A) tail to engage in double-stranded structures.

281 mRNA polyadenylation, the addition of a poly(A) tail to the 3'-end of pre-mRNA, is a process cri

282 ble miRNA-binding sites by looping the 3'UTR poly(A) tail to the bound miRISC and deadenylase.

283 The addition of the poly(A) tail to the ends of eukaryotic mRNAs is catalyze

284 The relationship of the poly(A) tail to translational control is intimately rela

285 ic acid (LNA)-containing oligo(dT) probes to poly(A) tails to maximize RNA capture selectivity and ef

286 The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and

287 ylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability.

288 l loop hybridizes with the 3'-polyadenylate (polyA) tail to sequester it from exonucleolytic attack.

289 Third, the poly(A) tail was necessary for maximal PUM repression.

290 polymerase, and the mRNA transcribed, with a poly(A) tail, was efficiently utilized in an in vitro tr

291 where the majority of the PAP I synthesized poly(A) tails were after the Rho-independent transcripti

292 regulated by RNAi in Chlamydomonas, very few poly(A) tails were detected in chloroplasts for the atpB

293 the number or sequence of mitochondrial mRNA poly(A) tails, where unexpectedly we found, in addition

294 Analysis of poly(A) tails, which destabilize chloroplast RNAs, indic

295 cleases for pre-tRNA substrates adding short poly(A) tails, which not only modulate the stability of

296 EMT/metastasis-related mRNAs to have shorter poly(A) tails while other mRNAs to have longer poly(A) t

297 Orb2 represses translation and removes mRNA poly(A) tails, while the oligomeric form enhances transl

298 turely translated: a transcript with a short poly(A) tail will remain untranslated, whereas elongatin

299 nstrate that our tool can precisely identify poly(A) tails with near perfect accuracy at the speed re

300 onfirm that cordycepin reduces the length of poly(A) tails, with some mRNAs being much more sensitive

301 Mature MALAT1 thus lacks a poly(A) tail yet is expressed at a level higher than man