ライフサイエンスコーパス: polyA

1 synthesis from RNA templates, followed by 3' polyA tailing of the single-stranded cDNA products and d

2 ylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability.

3 Interaction of 60S subunits with the 3' polyA is suggested.

4 ate an approximately 1-2.5-kb subset of [32P]polyA RNA molecules from a heterogeneous mixture ranging

5 ted between a cytomegalovirus promoter and a polyA signal to form a transcription cassette.

6 rget mRNAs to possess a 7-methyl G cap and a polyA tail, whereas increasing polyA tail length alone c

7 p mRNA terminating with a 900 bp 3'UTR and a polyA tail.

8 al E1A promoter with a cassette containing a polyA sequence and a human tyrosinase enhancer/promoter

9 mutation, a mononucleotide expansion from a polyA repeat tract (c.132dupA) that causes protein trunc

10 y conserved stem-loop sequence rather than a polyA sequence.

11 e end in a conserved stem-loop rather than a polyA tail.

12 Supt4h2, a processed intronless gene (with a polyA tail and a tandemly-duplicated 13 bp insertion sit

13 In recent years, poly adenine (polyA) DNA functionalized gold nanoparticles (AuNPs) fre

14 A sites, and the specific use of alternative polyA sites (APA) results in isoforms with variable 3'-u

15 ification in regulating proximal alternative polyA choice.

16 sites represent uncharacterized alternative polyA events and extensions of known transcripts in huma

17 nuclear mRNA revealed widespread alternative polyA site use (APA) in intestinally expressed genes.

18 and that, among transcripts with alternative polyA (APA) usage in both the brain and the liver, brain

19 ently use an alternative sixth exon (6c) and polyA signals near the middle of the former intergenic r

20 earrangements (21%), 5' truncation (74%) and polyA tails (88%).

21 ch as a splicing acceptor site, TATA box and polyA addition signal sequence, was further tested in si

22 We show that Cup and polyA-binding protein (PABP) interact physically with Sq

23 data generated by ENCODE in long polyA+ and polyA- fractions in the cell lines K562 and GM12878.

24 transposable elements in their promoter and polyA addition regions, and by the length of a CCA tande

25 striction endonuclease recognition sites and polyA or polyT tails.

26 transcription start sites, splice sites, and polyA sites) are defined and quantified for full-length

27 TAR and polyA form extended, coaxially stacked hairpins, consist

28 ction (RT-PCR) assays conducted on total and polyA+ RNA, as well as sequencing of cloned RT-PCR produ

29 Reverse transcription and polyA reverse transcriptase (RT)-PCR was performed, and

30 smsDGE involves a reverse-transcription and polyA-tailing sample preparation procedure followed by s

31 d 62% of protein-coding genes have annotated polyA sites.

32 TR switching directly without relying on any polyA annotations.

33 L1 elements are polyA retrotransposons which inhabit the human genome.

34 ur data suggest expansions in one of the ARX polyA tracts results in nuclear protein aggregation and

35 ed nonpoly(A) mRNA with the same kinetics as polyA(+) mRNA.

36 tor and adapter/linker sequences, as well as polyA/T tails.

37 duplications (TSDs) and longer than average polyA-tail length.

38 bases reflect time since loss of MMR because polyA deletions are stepwise.

39 and via an N-terminal zinc finger that binds polyA RNA specifically.

40 ate equivalent expression profiles from both polyA-selected and rRNA-depleted libraries.

41 ACK1/PKCbeta2 associated with polysome-bound polyA-mRNAs.

42 redominantly associated with polysome-bound, polyA-mRNAs that are being actively translated.

43 consecutive adenosine nucleotides, so-called polyA tracks, to the gene coding sequence of interest wi

44 ns that contain adenosine repeats, so-called polyA-leaders.

45 The canonical polyA signal was strongly enriched and positionally cons

46 ript in a domain downstream of the canonical polyA site.

47 e applied LQ-RNAseq to profile S. cerevisiae polyA+ transcripts, demonstrate the reproducibility of t

48 cts with a central stress granule component, polyA-binding protein (PABP).

49 domain region, and a variant in a conserved polyA+ signal sequence that alters the length of the 3'

50 models successfully classified constitutive polyA sites from a biologically relevant background (auR

51 2, forms a complex with PAB-1, a cytoplasmic polyA-binding protein, and that ATX-2 is required for de

52 g translation was, surprisingly, the Cytosol polyA- fraction in both cell lines.

53 s with sum total deletions at four different polyA loci of -32.7 bases in adenomas and -38.4 bases in

54 egulatory mechanisms underlying differential polyA preferences in multiple cell types has been hinder

55 brain transcripts preferentially use distal polyA sites, as reported, and also show higher proximal

56 mall amount of polyadenylation to downstream polyA sites when present, however, the majority of the a

57 fferent selection methods (typically, either polyA-selection or rRNA-depletion) omit different RNAs,

58 y that is specifically related to eukaryotic polyA polymerases is typified by yeast Trf4p and Trf5p p

59 Addition of exogenous polyA RNA to depleted optic nerve extracts yielded incre

60 hese findings further suggest that extensive polyA deletions common in MSI+ tumors likely reflect mul

61 ate annotation of positional information for polyA tails added posttranscriptionally.

62 CFIA is required for polyA site selection/cleavage targeting RNA sequences th

63 Sum total deletions in four polyA repeats were variable (between -17 to -45 bp) in 2

64 f human transcripts have multiple functional polyA sites, and the specific use of alternative polyA s

65 n vitro translation of PAD2 was monitored in polyA RNA depleted optic nerve extracts.

66 an N-terminal expanded polyalanine tract in polyA-binding protein nuclear 1 (PABPN1).

67 l G cap and a polyA tail, whereas increasing polyA tail length alone can increase miRNA silencing act

68 To understand the nature of intergenic polyA transcription, we first assessed its abundance usi

69 Importantly, we found that intestinal polyA sites that undergo APA tend to have U-rich and/or

70 e stability as affected by the length of its polyA anchor was another crucial aspect in our study.

71 ncation of its 3' UTR, including loss of its polyA tail, stabilized Bip1 mRNA, resulting in increased

72 teins, polyA-binding protein 1 and a 130 kDa polyA-mRNA binding protein (KIAA0217).

73 nes encode the only metazoan mRNAs that lack polyA tails, ending instead in a conserved 26-nt sequenc

74 icoid, and with genes encoding eIF4E, Larp1, polyA binding protein (PABP), and Ago2.

75 el sequencing of genomic DNA and full-length polyA(+) mRNA from single cells.

76 and RNA-seq data generated by ENCODE in long polyA+ and polyA- fractions in the cell lines K562 and G

77 We analyzed the long, polyA-selected, unstranded, deeply sequenced RNA-seq dat

78 Northern analysis of gastric mucosal polyA+ RNA revealed 7.5- and 4.1-kilobase transcripts, s

79 n human genes that we detected have multiple polyA sites in their 3'UTRs, with 49.3% having three or

80 ated region (UTR), but not the three natural polyA sites.

81 ession, the extent of deletions in noncoding polyA sequences were compared between 6 adenomas (all <

82 tumor-derived RNA (total or polyA+, but not polyA- RNA).

83 an approximately 8-fold increase in nuclear polyA RNA.

84 Analysis of polyA-selected RNAs and cDNA clones from several human c

85 Conventional methods rely on annotation of polyA sites; yet, such knowledge remains incomplete, and

86 Relative depletion of polyA signals in DoG regions correlates with increased l

87 vestigate and quantified the dissociation of polyA DNA on gold nanoparticles in diverse experimental

88 s, we wanted to understand the generality of polyA-expansion cytotoxicity by using yeast as a model o

89 ge remains incomplete, and identification of polyA sites is still challenging.

90 to maintain a good stability of this kind of polyA DNA-AuNPs nanoconjugates.

91 UTR switching without any prior knowledge of polyA annotations.

92 i-miRNA is predicted based on the mapping of polyA signals, and supported by cDNA/EST and ditags data

93 sion (DGE), enabling simultaneous mapping of polyA sites and quantitative measurement of their usage.

94 These quantitative maps of polyA usage in evolutionarily and functionally related s

95 ays that were hybridized with populations of polyA+ RNA sequence.

96 otocol to specifically probe the position of polyA sites in three human adult tissue types.

97 Given the prevalence of polyA tracts in eukaryotic proteomes, we wanted to under

98 olyalanine tract within the coding region of polyA binding protein nuclear 1 (PABPN1).

99 of single cells; the physical separation of polyA(+) mRNA from genomic DNA using a modified oligo-dT

100 io assembly of high-throughput sequencing of polyA(+) RNA (RNA-Seq) from a cohort of 102 prostate tis

101 We generated a series of polyA expansions in Arx and expressed these in cell cult

102 the cleavage of the pre-mRNA at the site of polyA addition.

103 itro, PKCbeta2 can phosphorylate a subset of polyA-mRNA-associated proteins that are also phosphoryla

104 In general, usage of polyA sites is more similar within the same tissues acro

105 They demonstrate the utility of polyA DNA and real-time PCR for measurement of gene sign

106 Subtractive hybridization was conducted on polyA PCR-amplified RNA to isolate genes expressed by co

107 -mRNA cleavage and polyadenylation sites, or polyA sites, including more than 3000 sites that have pr

108 DC pulsed with unfractionated RNA (total or polyA+) from OVA-expressing tumor cells were as effectiv

109 h DC pulsed with tumor-derived RNA (total or polyA+, but not polyA- RNA).

110 indicating a requisite role for mRNA in Pab1 polyA-expansion toxicity.

111 ve AAA codons, sensing of prematurely placed polyA tails by a specialized RNA-binding protein is a no

112 l loop hybridizes with the 3'-polyadenylate (polyA) tail to sequester it from exonucleolytic attack.

113 s to suggest the presence of polyadenylated (polyA) transcripts originating from presumed intergenic

114 aled widespread alternative polyadenylation (polyA) in eukaryotes, leading to various mRNA isoforms d

115 and used it to globally map polyadenylation (polyA) sites in 24 matched tissues in human, rhesus, dog

116 f the transactivation (TAR)/polyadenylation (polyA), primer-binding site (PBS), and Psi-packaging dom

117 expansions in their endogenous polyalanine (polyA) tracts.

118 Recently, polyalanine (polyA) tract expansions in the Aristaless-related homeob

119 ions that polyadq uses to evaluate potential polyA signals.

120 cific leader activity that underlie poxvirus polyA-leaders.

121 In our tests, polyadq predicts polyA signals with a correlation coefficient of 0.413 on

122 ulin and at least two mRNA-binding proteins, polyA-binding protein 1 and a 130 kDa polyA-mRNA binding

123 n operon of Mycoplasma, a domain of putative polyA polymerases in Synechocystis and Aquifex, PRUNE of

124 rse transcription polymerase chain reaction, polyA tail, 3' rapid amplification of complementary DNA

125 sulin receptor RNA in both total RNA and RNA polyA+ pools relative to normal and myopathic control mu

126 ions (maximum of -12 bp) occurred in similar polyA sequences in MMR-deficient mice (mlh1-/-) up to 47

127 itioning transgene element to share a single polyA sequence for termination.

128 rounding the cleavage/polyadenylation sites (polyA sites), which are frequently constrained by sequen

129 signal being nearly absent at brain-specific polyA sites.

130 while single nucleotides or single-stranded polyA or polyC constructs do not.

131 ng allows Pan3 to supply Pan2 with substrate polyA RNA, facilitating efficient mRNA deadenylation by

132 ction lacking PrPSc template seed, synthetic polyA RNA molecules induce hamster HaPrPC to adopt a pro

133 a broader fraction of the transcriptome than polyA-selected libraries.

134 siRNAs bind the target messenger RNA at the polyA signal and are capable of redirecting a small amou

135 adic tumours that exhibit instability at the polyA tract in the TGFbetaRII gene and to 35% per allele

136 ovl4, which contained 1794 bp (excluding the polyA tail), including 909 bp of coding region that enco

137 In our initial case, we expanded the polyA tract within the native yeast poly(Adenine)-bindin

138 paration of the transposase element from the polyA sequence after transposition leads to its deactiva

139 icity that correlated with the length of the polyA expansion.

140 an RNA helicase, and Cid12, a member of the polyA polymerase family, in a complex that has RNA-direc

141 onsistently detect the ATTAAA variant of the polyA signal.

142 dulated by changing sequence features of the polyA tracks.

143 hesize involves stalling of ribosomes on the polyA tail.

144 g by synthesis methods, we have surveyed the polyA+ transcripts from four stages of the nematode Caen

145 h the ENE's ability to (i) interact with the polyA tail, (ii) inhibit deadenylation in vitro, and (ii

146 ed set of base-pairing interactions with the polyA tail.

147 confirmed by probing a multiple human tissue polyA(+)RNA.

148 ctivated PKCbeta2 to mRNP complexes bound to polyA-mRNAs is involved in activity-triggered control of

149 real-time polymerase chain reaction (PCR) to polyA cDNAs prepared from 106 archived human frozen lymp

150 genes, selected from microarray studies, to polyA cDNAs prepared from 60 archived human frozen lymph

151 knocked down, spliced mRNA, as well as total polyA+ RNA, accumulates in nuclear speckle domains.

152 Encounter with translated polyA segments by ZNF598 triggered ubiquitination of sev

153 ontext of viral replication when an upstream polyA was included.

154 Using polyA capture, 3' rapid amplification of complementary D

155 ction of OVA-specific CTL was abrogated when polyA+ RNA from OVA-expressing cells was treated with an

156 RP with stress granules and colocalizes with polyA binding protein in a variant-dependent manner.