ライフサイエンスコーパス: polybasic

1 by an electrostatic compensation between the polybasic 23-30 region and the alpha3 electronegative su

2 This depends on the polybasic activation loop as well as a conserved hydroph

3 We identified a role for a conserved polybasic amino acid motif in an N-terminal domain previ

4 ma membrane and depends on the presence of a polybasic amino acid segment at the N terminus of PrP.

5 he intracerebral pathogenicity index and the polybasic amino acid sequence at the fusion protein clea

6 en characterized, but, because it contains a polybasic amino acid sequence, it potentially interacts

7 A sequence analysis revealed polybasic amino acids in the hemagglutinin connecting pe

8 Its effects involve both a polybasic amino-terminal segment and a proline-rich core

9 We removed the polybasic aminoacids that are associated with high virul

10 CaM specifically targeted the highly polybasic anchor region of the K-Ras4B HVR that stably w

11 ply that relevant FTI targets will lack both polybasic and potentially geranylgeranylated methionine-

12 tes Orai1 by a different mechanism since the polybasic and S/P domains of STIM1 are not required for

13 ration of hitherto unknown highly acidic and polybasic bis(difluoromethylene)triphosphoric acid 1 usi

14 was present, supporting a role for the Rac1 polybasic C terminus in binding to the membrane.

15 both the Ras homology (core) domain and the polybasic C terminus.

16 proteins from human H1N1 strains that lack a polybasic cleavage motif.

17 proteins of the H5 and H7 subtypes that have polybasic cleavage motifs, this study demonstrates that

18 n influenza virus HA proteins that contain a polybasic cleavage site from pH-induced conformational c

19 sion of a hemagglutinin protein in which the polybasic cleavage site had been removed.

20 In contrast, introduction of a polybasic cleavage site into Israel810 HA leads to pseud

21 To this end, we introduced a polybasic cleavage site into the HA of several low-patho

22 ines expressing either HPAIV HA in which the polybasic cleavage site was replaced with that from a lo

23 ed to distinguish between compatibility of a polybasic cleavage site with H5/H7 HA only and unique pr

24 human influenza virus HA proteins that lack polybasic cleavage sites.

25 termini, containing N-myristate and either a polybasic cluster (in Src) or palmitoylation sites (e.g.

26 and 1.9 A, respectively, unveiling that the polybasic cluster formed by strands beta3-beta4 is invol

27 Similarly, mutations in the polybasic cluster of MA that disrupt Gag polarization ca

28 he presence of a key glutamic residue in the polybasic cluster of synaptotagmin 1 that abolishes the

29 The C-terminal polybasic cluster of the Rab35 HVD is essential for plas

30 requires a pleckstrin homology domain and a polybasic cluster that bind to phosphoinositide lipids.

31 ion is mediated by an HE motif followed by a polybasic cluster that is conserved in transcriptional C

32 To test whether these polybasic clusters bind negatively charged phosphatidyli

33 Unexpectedly, proteins with polybasic clusters dissociated from the PM only when bot

34 rimary structure of this motif is similar to polybasic clusters known to interact with polyphosphoino

35 Polybasic cofactors lowered the K(m) for diacylglycerol

36 ies showed that acidic phospholipids recruit polybasic cofactors to the vesicle surface but have litt

37 afts through electrostatic interactions with polybasic cytoplasmic proteins, such as GAP-43, which bi

38 The lipin proteins each contain a conserved polybasic domain (PBD) composed of nine lysine and argin

39 (PH) domain and an adjacent carboxy-terminal polybasic domain [3] [9].

40 The polybasic domain alone increases the affinity of Ras for

41 targeting R7BP to the plasma membrane with a polybasic domain and an irreversibly attached lipid inst

42 es, was replaced with that of the human Rac2 polybasic domain containing only three basic residues, a

43 Thus, polybasic domain differences account for the disparate a

44 Here we demonstrate that a polybasic domain immediately upstream of palmitoylated c

45 These data demonstrate the key role of the polybasic domain in controlling stress-regulated exon pa

46 FHOS interacted with the polybasic domain in the Rac1 C terminus in a guanine nuc

47 olycysteine sequences, and that the adjacent polybasic domain is not required for Galpha palmitoylati

48 Thus, the composition of the polybasic domain is sufficient for determining Rac isofo

49 ent and prenyl chain length modifies nascent polybasic domain lipid preferences.

50 have taken a closer look at the role of the polybasic domain of Cdc42 in its ability to bind to memb

51 ltiple substitutions within the hydrophilic, polybasic domain of gp91(phox) encompassed by residues 8

52 phosphorylation regulates the ability of the polybasic domain of lipin 1 to recognize di-anionic PA a

53 Our studies suggest that the polybasic domain of Rac is a novel effector domain that

54 Moreover, replacing the highly charged polybasic domain of Rac1 with the less charged domain of

55 -Ras polybasic domain, we show that either a polybasic domain or an alternatively prenylated CAAX ren

56 A polybasic domain proximal to the CaaL box motif induced

57 n of a negatively charged phosphate into the polybasic domain reduced interaction of ARNO with membra

58 se C; (d) reveal that phosphorylation of the polybasic domain regulates affinity for F-actin and Ca(2

59 ic amino acids to acidic residues within the polybasic domain results in inhibition of channel palmit

60 t that palmitoylation of the AKAP N-terminal polybasic domain targets it to postsynaptic lipid rafts

61 itic spine plasma membranes by an N-terminal polybasic domain that binds phosphoinositide lipids, F-a

62 of the STIM1 C terminus, thereby releasing a polybasic domain that promotes STIM1 recruitment to ER-P

63 expression of a Rac2 derivative in which the polybasic domain was replaced with that of Rac1 did not

64 s electrostatic interaction of the lipidated polybasic domain with anionic phospholipids in the plasm

65 ster of positively charged residues (i.e. a "polybasic domain"), directly preceding their geranylgera

66 ared to be mediated by the carboxyl-terminal polybasic domain, and the specific GTPase-activating eff

67 ons of Ras background, CAAX motif, and K-Ras polybasic domain, we show that either a polybasic domain

68 ic Rac1 protein in which the Rac1 C-terminal polybasic domain, which contains six lysines or arginine

69 single serine residue within the core of the polybasic domain, which results in channel inhibition, a

70 undergoes monoubiquitination in a conserved polybasic domain.

71 cal interaction of PIPKI-alpha with the Rac1 polybasic domain.

72 rises a farnesyl-cysteine-methyl-ester and a polybasic domain.

73 itively charged STIM1((684)KK(685)) in STIM1 polybasic domain.

74 phorylates K-Ras at Ser181 in the C-terminal polybasic domain.

75 e, S392, located within the carboxy-terminal polybasic domain.

76 ly GTPases mediated by the carboxyl-terminal polybasic domain.

77 fication that operates in conjunction with a polybasic domain.

78 et of the plasma membrane via a farnesylated polybasic domain; however, the structural details of the

79 In particular, polybasic domains represent an attractive mechanism to d

80 omers is the composition of their C-terminal polybasic domains.

81 le lipid modification, or hydrophobic and/or polybasic domains.

82 y oligocations such as polyamines, melittin, polybasic drugs, oligolysines, and oligoarginines.

83 We investigated the hypothesis that the polybasic effector domain (ED) of the abundant intracell

84 the combined action of myristoylation and a polybasic effector domain, which binds phospholipids and

85 For the C2B domain, contact occurs in the polybasic face and sites opposite the Ca(2+)-binding loo

86 switch to modulate the accessibility of the polybasic face of C2B and control interactions of syt1 w

87 The polybasic face of C2B does not interact with the membran

88 It contains a polybasic farnesylated C-terminus that is required for t

89 tering into microdomains are mediated by its polybasic farnesylated C-terminus.

90 All isolates had polybasic fusion (F)-protein cleavage sites and were sho

91 Taken together, in the presence of a polybasic HA cleavage site, non-H5/H7 HA can support a h

92 he HA serotypes H5 or H7 by acquisition of a polybasic HA cleavage site.

93 r unique predisposition for acquisition of a polybasic HA cleavage site.

94 loid deposits, relative to similarly charged polybasic heparin-reactive peptides, because it adopted

95 ity enables CaM to orient efficiently to the polybasic HVR anchor, which is partially diffused into t

96 onical form (ATXbeta) in having a 52-residue polybasic insertion of unknown function in the catalytic

97 The exact function of the polybasic juxtamembrane region (5RK) of the plasma membr

98 ith phosphoinositides, the function of Syx's polybasic juxtamembrane region (5RK) remains unclear.

99 AREDelta60, but the interaction requires the polybasic juxtamembrane region of syntaxin-1 and is not

100 Our findings highlight the importance of the polybasic juxtamembrane sequence in regulating the oncog

101 In the absence of PIP 2, the polybasic lipid binding site on the beta3-beta4 hairpin

102 cherichia coli) binds to PtdIns(4,5)P2 via a polybasic lysine patch in the C2B domain, which may prom

103 dSer, and an increase in the affinity of the polybasic lysine patch to phosphatidylinositol-4,5-bisph

104 trate sites within the AKAP79/150 N-terminal polybasic membrane-cytoskeletal targeting domain were ph

105 Here we show that a membrane-proximal, polybasic motif (PBM) in the cytosolic tail of p14 is es

106 We recently reported that a polybasic motif (PBM) in the cytosolic tail of reptilian

107 lserine (PS)-containing bilayers through its polybasic motif (PBM).

108 izes to the plasma membrane via a C-terminal polybasic motif and interacts with calcium channel beta

109 ontributions of its two distinct elements, a polybasic motif and palmitoylated cysteines, which when

110 ffector that directly binds PIP(2) through a polybasic motif and PIP(2) binding activates IQGAP1, fac

111 We identify a role for the lipin1 polybasic motif as both a lipid binding motif and a prim

112 id binding modules, such as PH domains, this polybasic motif binds PIP(2) in a multivalent, cooperati

113 We identify a polybasic motif in the proximal C terminus of retigabine

114 e used pharmacological agents and lipin1beta polybasic motif mutants to explore the role of PA-mediat

115 According to our models, this polybasic motif of the I-II linker forms a straight alph

116 /cytoplasmic shuttling and that a C-terminal polybasic motif proximal to the palmitoylation acceptor

117 oop identified several unique residues and a polybasic motif that contribute to the catalytic activit

118 his binding involves a previously identified polybasic motif that mediates activation of the enzyme b

119 selectivity to function in concert with the polybasic motif to target the protein to PI(4,5)P2-rich

120 Studies using lipin1beta polybasic motif variants establish that this region is a

121 modeling to predict the conformation of this polybasic motif, immunofluorescence microscopy and live

122 ld can be tuned by varying the length of the polybasic motif.

123 and stimulation of catalysis mediated by the polybasic motif.

124 We also found that polybasic motifs present in the cytoplasmic tails of CD4

125 lytic domain in addition to the farnesyl and polybasic motifs.

126 of plasma membrane localization of alpha(q) polybasic mutants by introduction of a site for myristoy

127 Furthermore, the deletion of the short polybasic N-terminal region 23-30 was found to reduce th

128 A peptide containing the prototypical polybasic NLS sequence of the SV40 large T-antigen was a

129 alization of IFN-gamma is driven by a simple polybasic nuclear localization sequence (NLS) in its COO

130 ound that PIP(2) bound to the well conserved polybasic patch of the C2B domain with an apparent disso

131 in variant that is linked to a farnesylated, polybasic peptide corresponding to the K-Ras4B C terminu

132 farnesyl cysteine methyl ester adjacent to a polybasic peptide segment, to the cytosolic face of the

133 Small polybasic peptides derived from the transduction domains

134 it has been proposed that certain endogenous polybasic PKC substrates may activate PKC in cells by th

135 Here we identified a polybasic plasma membrane binding motif, consisting of f

136 A mutant STIM1 lacking the C-terminal polybasic PM-targeting motif oligomerized after Ca(2+) s

137 nslocation is reversible and mediated by the polybasic-prenyl membrane targeting motif of KRas.

138 We conclude that the polybasic-prenyl motif acts as a Ca2+/CaM-regulated mole

139 location occurs through sequestration of the polybasic-prenyl motif by Ca2+/calmodulin (Ca2+/CaM) and

140 nity, consistent with a potential role for a polybasic protein or protein domain in the activation of

141 dent substrate protamine sulfate, which is a polybasic protein that activates PKC by a novel mechanis

142 of PKC isozymes at subcellular sites rich in polybasic proteins, it has been proposed that certain en

143 These substrates share a bipartite polybasic recognition determinant (BPR) flanking a Cdk1

144 nd Rho small GTPases possessing a C-terminal polybasic region (PBR) are vital signaling proteins whos

145 One helix contains a polybasic region (PBR) composed of Arg-164, Arg-168, Lys

146 A polybasic region (PBR) in the C terminus blocks interact

147 The COOH-terminal polybasic region (PBR) of Rac1, a Rho family GTPase memb

148 GPCRs) phosphorylates S-179 and S-180 in the polybasic region (PBR) of Rap1B, which inhibits Rap1B bi

149 In this study, we identified a small polybasic region (PBR) preceding the RhoGAP domain that

150 tly identified residues within the ST8Sia-IV polybasic region (PBR) that are required for neural cell

151 nge by small GTPases containing a C-terminal polybasic region (PBR), such as Rac1 and RhoA.

152 ver, our studies suggest that the N-terminal polybasic region 23-30 is essential for effective foldin

153 afficking of GTPases containing a C-terminal polybasic region and demonstrated that SmgGDS-607 intera

154 , Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids

155 by an electrostatic interaction between its polybasic region and the endosomal acidic phospholipids,

156 ytically cleaves EC-SOD in the middle of the polybasic region and then requires an additional carboxy

157 phosphorylates K-Ras4B on serine 181 in the polybasic region and thereby induces translocation from

158 olycysteine sequence along with the adjacent polybasic region are both important for G16alpha-mediate

159 These findings establish the Pf1 polybasic region as a phosphoinositide-binding module an

160 hat mice expressing PrP deleted for a short, polybasic region at the N terminus (residues 23-31) disp

161 A polybasic region at the Rga6 C-terminus is responsible f

162 Furthermore, this polybasic region binds specifically to PI(3)P when fused

163 We show that a triproline N-terminal to the polybasic region contributes to the NLS, which is crypti

164 The presence of an upstream polybasic region does not significantly affect GGTase I-

165 Thus, the presence of an upstream polybasic region enhances the dual prenylation of these

166 rast to known GEFs requires RhoA to retain a polybasic region for activation.

167 nase, one of the effectors that requires the polybasic region for interaction, is necessary for effic

168 Here we identify a polybasic region in Gic2 adjacent to the Cdc42/Rac inter

169 imeric G protein subunit alpha(q) contains a polybasic region in its N terminus that contributes to p

170 A polybasic region in the carboxyl terminus distinguishes

171 art of the plasma membrane binding signal, a polybasic region in the matrix protein, was mutated.

172 s the octapeptide repeats and amino-terminal polybasic region in the prion protein, but not its endoc

173 ulatory protein N-WASP by binding to a short polybasic region involved in N-WASP autoinhibition.

174 r demonstrated that the conserved C-terminal polybasic region is important for specific phosphoinosit

175 Previous work suggested that a polybasic region located prior to the conserved polysial

176 Here, the role of the N-terminal polybasic region of alpha(q) in signaling was addressed.

177 (2+)-calmodulin and F-actin; (c) show that a polybasic region of DAKAP200 is a substrate for protein

178 We show that the polybasic region of Rsr1 is necessary for the efficient

179 and Nox4 interactions were dependent on the polybasic region of the B-loop.

180 utative Syt1-SNARE/PIP2 coupling through the polybasic region of the C2B domain is critical for vesic

181 The polybasic region of the Rac1 C terminus functions both a

182 , our results indicate that the ST8SiaIV/PST polybasic region plays a critical role in substrate reco

183 by protein kinase C (PKC) of S181 within the polybasic region promotes rapid dissociation of K-Ras fr

184 Rac1(PBRM), a mutant lacking the C-terminal polybasic region required for Rac1 association with the

185 Replacing two polybasic region residues, Arg(82) and Arg(93), eliminat

186 cids in this conserved polysialyltransferase polybasic region that are critical for the protein-speci

187 The second is a 35-amino acid polybasic region that contains seven basic residues and

188 Surprisingly, a polybasic region that follows the PHD1 is necessary for

189 strates of these enzymes contain an upstream polybasic region that is proposed to increase the affini

190 high propensity for trimer formation, and a polybasic region that precedes the C-terminal prenylatio

191 demonstrate that the addition of an upstream polybasic region to a peptide substrate enhances the bin

192 ne residue at position 186 in the C-terminal polybasic region were found to possess a self-stimulator

193 on of ING2 (consisting of a PHD finger and a polybasic region) revealed a number of complementary sur

194 ctive ST8SiaIV/PST proteins that include the polybasic region, but not those that lack this region, c

195 B-loop, which in Nox1-4 contains a conserved polybasic region.

196 ma membranes by a farnesyl lipid group and a polybasic region.

197 ite of palmitoylation as well as a bipartite polybasic region.

198 l sequences (K(K/R)X(K/R)) in the C-terminal polybasic regions (PBRs) of some Rac and Rho isoforms.

199 gnaling triggered by the interaction between polybasic regions and phosphoinositides.

200 Polybasic regions are often associated with nonspecific

201 By exchanging the polybasic regions between different PHD fingers we show

202 f ST8Sia IV sequences revealed two conserved polybasic regions that might interact with the NCAM acid

203 g; individual cysteine residues, but not the polybasic regions, determine lipid modification and subc

204 rating NADPH oxidase, contains two conserved polybasic regions: one N-terminal (PBR-N), located betwe

205 Both the polybasic residues and the adjacent prenylation motif ar

206 Deletion or mutation of the polybasic residues drastically decreased its intrinsic G

207 phospholipid PI(3,4,5)P3 and the stretch of polybasic residues preceding the RhoGAP domain regulates

208 h the sequence of the classical NLS contains polybasic residues that are recognized by importin-alpha

209 Polybasic secretagogues such as mastoparan, compound 48/

210 inker interactions occlude the action of the polybasic segment and that its functional availability i

211 Here, we identified a short polybasic segment at the boundary of the guanylate kinas

212 The linker upstream from the polybasic segment, but not the N- and C-terminal variabl

213 IFN-gamma (IFN-gamma(95-132)) containing the polybasic sequence 126RKRKRSR132 was capable of specifyi

214 ich the proline-rich region was fused to the polybasic sequence from the HIV Tat protein to facilitat

215 The polybasic sequence in the C terminus of RhoA is essentia

216 Notably, the presence of a polybasic sequence in the PSGL-1 cytoplasmic domain sign

217 alpha, and G16alpha) in particular utilize a polybasic sequence of amino acids in their N terminus to

218 Mutations in a polybasic sequence on the side of the C2B domain beta-sa

219 -GG, which contains a prenylation site and a polybasic sequence similar to K-ras.

220 GEF-independent mechanism requires the Rho1 polybasic sequence that binds to acidic phospholipids, i

221 The contaminants bind to a polybasic sequence that has been previously implicated i

222 Nuclear import was abolished when the above polybasic sequence was deleted.

223 e IFN-gamma(95-125), which is deleted in the polybasic sequence, further confirming that the NLS prop

224 ng potential depends on the integrity of the polybasic sequence.

225 at functions in conjunction with an adjacent polybasic sequence.

226 residues where Rac1 but not Rac2 contains a polybasic sequence.

227 d CD44 with assembling HIV-1 which relies on polybasic sequences in HIV-1 Gag and in cytoplasmic doma

228 Translation arrest by polybasic sequences induces ribosome stalling, and the a

229 nuclear targeting is mediated in part by two polybasic sequences present at the C-terminal end of SKC

230 sociation with assembling HIV-1 Gag in which polybasic sequences present in the cytoplasmic tails of

231 o the binding of phosphatidate to a specific polybasic site within the kinase domain of Raf-1.

232 In this model, the polybasic strand of C2B forms the membrane binding surfa

233 mutation of a single basic residue within a polybasic stretch of the DEP domain, which abolishes tra

234 initiate chemical signaling, as well as the polybasic stretches that regulate signal potentiation.

235 luenza A virus infections, to both mono- and polybasic subtypes.