ライフサイエンスコーパス: polygalacturonase

1 otyls, which overexpresses a gene encoding a polygalacturonase.

2 The polygalacturonase 11 promoters (GmPG11a/b) include the S

3 We found that LAX3 and polygalacturonase, a LAX3-induced cell wall-modifying en

4 putative target RNAs of AtC3H14, including a polygalacturonase, a well-known cell wall modifying gene

5 wall, and PGX2(AT) plants show higher total polygalacturonase activity and smaller pectin molecular

6 sed, truncated version of PGX2 also displays polygalacturonase activity in vitro.

7 Polygalacturonase activity was quantified indirectly by

8 or lacking PGX1 display alterations in total polygalacturonase activity, pectin molecular mass, and w

9 erologously expressed PGX1 displays in vitro polygalacturonase activity, supporting a function for th

10 ccharomyces cerevisiae) and found to exhibit polygalacturonase activity.

11 ion allele showed a significant reduction in polygalacturonase activity.

12 r, the extent to which pectin degradation by polygalacturonases affects stem development and secondar

13 This is the first structure of a polygalacturonase and comprises a 10 turn right-handed p

14 roperties of the substrate-binding clefts of polygalacturonase and pectate lyase, which bind and clea

15 's degradation by the combined activities of polygalacturonase and pectate lyase.

16 ta-galactosidase, alpha-arabinofuranosidase, polygalacturonase and pectin methylesterase, were measur

17 nolytic enzymes pectate lyase, pectin lyase, polygalacturonase and rhamnogalacturonase, and unlike th

18 ronide fragments produced by wound-inducible polygalacturonase and that the resulting H(2)O(2) acts a

19 by specific biochemical function, with endo-polygalacturonases and endo-rhamnogalacturonases forming

20 ed genes induced by B. cinerea are LePG (for polygalacturonase) and LeExp1 (for expansin), which enco

21 Group 11 pollen allergens, polygalacturonase, and actin depolymerizing factor were

22 basal levels of extracellular pectate lyase, polygalacturonase, and cellulase as well as those of tra

23 polymer was resistant to proteinase K, endo-polygalacturonase, and endo-xylanase III (GH11 family) b

24 ce factors such as zinc metalloproteases and polygalacturonase are known to be secreted by the T2SS.

25 stinct, apparently ancient clades, while exo-polygalacturonases are more widely distributed.

26 Using the promoter of a polygalacturonase (At2g41850), active primarily in cells

27 RNA gel blot analysis of cellulase, polygalacturonase beta-subunit, 1,3-beta-glucanase, and

28 s defense proteins by inhibiting fungal endo-polygalacturonases, but enzyme assays with extracts of A

29 t produces very low levels of pectate lyase, polygalacturonase, cellulase, protease, and E. carotovor

30 tomatal movement and greatly altered PME and polygalacturonase (EC 3.2.1.15) activity, resulting in a

31 ith two forms of pectin lyase (EC 4.2.2.10), polygalacturonase (EC 3.2.1.15), or all combinations.

32 degradation by endogenous pectinases such as polygalacturonases, few of which have been functionally

33 and AtPGIP2 encode functional inhibitors of polygalacturonase from Botrytis, and their overexpressio

34 The crystal structure of the 40-kDa endo-polygalacturonase from Erwinia carotovora ssp. carotovor

35 nd glyoxyl groups and evaluated to stabilize polygalacturonase from Streptomyces halstedii ATCC 10897

36 where the promoter of an abscission-specific polygalacturonase gene (At2g41850/ARABIDOPSIS DEHISCENCE

37 , we describe mutations in the predicted exo-polygalacturonase gene NIMNA (NMA) that lead to cell elo

38 ato (Lycopersicon esculentum cv Ailsa Craig) polygalacturonase genes TAPG1 (LYCes;Pga1;2) and TAPG4 (

39 ther the expression of the Aspergillus niger polygalacturonase II (AnPGII; 35S:AnPGII plants) or a mu

40 These results reveal new roles for polygalacturonases in plant development.

41 cyanins and flavanols in Nebbiolo, for which polygalacturonase, individually or in multi-enzyme blend

42 al PG was fused with a gene encoding a plant polygalacturonase-inhibiting protein (PGIP) and expresse

43 A cDNA encoding polygalacturonase-inhibiting protein (PGIP) from mature

44 Polygalacturonase-inhibiting proteins (PGIPs) are plant

45 to study the interactions of EPG-II and the polygalacturonase inhibitor protein (PGIP).

46 teins that are composed of LRRs, such as the polygalacturonase inhibitor proteins (PGIP) of plants.

47 rot antifreeze protein is similar to that of polygalacturonase inhibitor proteins and contains leucin

48 defense genes, lipoxygenase, catalase 3 and polygalacturonase-inhibitor protein.

49 We named this gene POLYGALACTURONASE INVOLVED IN EXPANSION1 (PGX1).

50 We designated this gene as POLYGALACTURONASE INVOLVED IN EXPANSION2 (PGX2), and the

51 We characterized POLYGALACTURONASE INVOLVED IN EXPANSION3 (PGX3), which i

52 nservation indicates that the active site of polygalacturonase is between these two loop regions, and

53 assessed in transgenic fruit with suppressed polygalacturonase (LePG) and expansin (LeExp1) expressio

54 there was at least a 1000-fold reduction in polygalacturonase levels in those plants bearing Ds inse

55 om strawberry fruits were digested with endo-polygalacturonase M2 from Aspergillus aculeatus and visu

56 uded HecA hemagglutinin family adhesion, Peh polygalacturonase, new effector HopPtoC(EA), and membran

57 creased synthesis and export of enzymes like polygalacturonase, pectin esterase, and other enzymes im

58 by their degradability by the pectic enzymes polygalacturonase, pectinmethylesterase and rhamnogalact

59 tate lyase isozymes (Pels), cellulase (Cel), polygalacturonase (Peh) and protease (Prt).

60 produces extracellular pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (

61 extracellular enzymes (pectate lyase [Pel], polygalacturonase [Peh], cellulase [Cel], and protease [

62 Pectin methylesterase (PME) and polygalacturonase (PG) activities were higher in A-treat

63 en-derived pectin-degrading enzymes, because polygalacturonase (PG) activity has not been reported in

64 Transgenic experiments show that polygalacturonase (PG) activity is largely responsible f

65 In tomato, polygalacturonase (PG) and expansin (Exp) are among the

66 ansformed with a tomato leaf wound-inducible polygalacturonase (PG) beta-subunit gene in the antisens

67 loning of a tomato (Lycopersicon esculentum) polygalacturonase (PG) cDNA, TAPG1, expressed during lea

68 Moreover, IDL6 promotes the expression of a polygalacturonase (PG) gene, ADPG2, and increases PG act

69 ying enzymes pectin-methylesterase (PME) and polygalacturonase (PG) in tomato fruit was tailored by p

70 pectins by tomato (Lycopersicon esculentum) polygalacturonase (PG) in vitro is more extensive than t

71 Polygalacturonase (PG) is the major enzyme responsible f

72 responds with an increase in wound-inducible polygalacturonase (PG) mRNA and enzyme activity previous

73 -transcriptional silencing of the endogenous polygalacturonase (PG) sense gene and a truncated homolo

74 Polygalacturonase (PG) showed better association with ce

75 transformants harboring a stably integrated polygalacturonase (PG) transgene driven by a constitutiv

76 al knockout mutations in the gene for tomato polygalacturonase (PG), a critical enzyme in fruit ripen

77 Here, cypress pollen allergens, especially a polygalacturonase (PG), were further characterized using

78 ruit have been attributed to the activity of polygalacturonase (PG).

79 r cell wall disassembly processes in plants, polygalacturonases (PGs) may be involved.

80 ta by partial inhibition of pathogen-encoded polygalacturonases (PGs).

81 r the control of the fruit ripening-specific polygalacturonase promoter to divert the metabolic flux

82 ol3 transposon is also present in the tomato polygalacturonase promoter to which it conferred regulat

83 anded RNA, the polymer xanthan, and enzymes (polygalacturonase, protease, cellulase).

84 1g33430, 4-coumarate-coenzyme A ligase 4CL3, polygalacturonase QUARTET3, novel gene At5g58100, and nu

85 oduce ethylene, and have increased levels of polygalacturonase RNA.

86 on, we identified a gene encoding a putative polygalacturonase that, when overexpressed, resulted in

87 Polygalacturonase was activated at 130 MPa, inactivated

88 -glucose-1-phosphate uridylyltransferase and polygalacturonase were observed in SPP and TOT fractions

89 s) are plant proteins that counteract fungal polygalacturonases, which are important virulence factor

90 regions, and comparison of the structure of polygalacturonase with that of rhamnogalacturonase A fro

91 Enzymatic digestion of PD with 1,4-alpha-d-polygalacturonase yielded the fraction PD-E.