ライフサイエンスコーパス: polylinker

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1 A LUC mRNA with a 17-nucleotide polylinker 5' untranslated region (UTR) was expressed 10

2 an insertion of 60 nucleotides coding for a polylinker, a 2B/2C cleavage site for HAV protease 3Cpro

3 DNA sequences separated by different length polylinkers, allowing simple differentiation by polymera

4 ncoding the p40 and p35 subunits linked by a polylinker and, using a retroviral vector, transfected J

5 ternal fusion exon consisting of alpha cMHC, polylinker, and hRen sequences.

6 e replaced large T antigen gene (Tag) with a polylinker, and inserted firefly luciferase, controlled

7 pPL1 contains a useful polylinker, can be directly conjugated from Escherichia

8 nation, or in triple combination by a unique polylinker, had no effect on dex inducibility.

9 ouble-stranded DNA PCR fragment containing a polylinker in its middle is digested with two restrictio

10 The reporter system consists of a polylinker of various unique restriction endonuclease re

11 Each CRIM plasmid has a polylinker or one of several promoters for ectopic expre

12 ut also the conserved serine residues of the polylinker region in Smad3, suggesting that CLP-1-mediat

13 e RecA intein was inserted in-frame into the polylinker region of an inducible lacZ alpha-ccdB fusion

14 e of four tandem copies of TTTAAAGCCG in the polylinker region of pLITMUS28 resulted in a DNA molecul

15 (a gfp derivative [Clontech]) preceded by a polylinker region.

16 essor gene with newly incorporated expressed polylinker restriction sites still functions as a repres

17 or the archaeal histone HMfB relative to the polylinker sequence, and the dominant, quantitative cont

18 The presence of CaMV and polylinker sequences at the 5' end of the PetE leader ma

19 required the deletion of CaMV 5' leader and polylinker sequences from the constructs.

20 3- to 60-fold above those in which synthetic polylinker sequences replaced the lat52 5'-UTR.

21 Access to the database of vector polylinker sequences via sequence similarity searching i

22 Using a panel of 180 vector polylinker sequences, we found 0.36% or 3029 vector-matc

23 on fragments that were cloned into the pUC19 polylinker site; each fragment was also obtained in both

24 ddition of an KmR-encoding gene and flanking polylinker sites, greatly simplifying construction of ge

25 quence, variable heavy chain, glycine-serine polylinker, variable light chain, and biotin mimic pepti

26 We also describe several new bacterial polylinker vectors derived from pUC21 (ampicillin resist

27 asmid vector has been prepared featuring the polylinker with two flanking priming sites to bi-directi

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