ライフサイエンスコーパス: polyribosome

1 ly promotes the association of this RNA with polyribosomes.

2 exported to the cytoplasm and is present in polyribosomes.

3 in addition to promoting ATX2 assembly with polyribosomes.

4 over, some CUTs appear to be associated with polyribosomes.

5 toplasmic 61-kDa species was associated with polyribosomes.

6 hat FMRP forms complexes with cortical brain polyribosomes.

7 aining mRNA and promote its association with polyribosomes.

8 both in nucleus, and in the cytoplasm on the polyribosomes.

9 sequence were preferentially associated with polyribosomes.

10 h appearance of specific mRNA transcripts in polyribosomes.

11 component of both soluble and membrane-bound polyribosomes.

12 Furthermore, Tap, but not NXT is detected in polyribosomes.

13 oprotein (mRNP) complex that associates with polyribosomes.

14 ibonucleoprotein particle is associated with polyribosomes.

15 d FMRP is associated with apparently stalled polyribosomes.

16 ng protein that is primarily associated with polyribosomes.

17 n a soluble form, as well as associated with polyribosomes.

18 llular localization of the luc transcript to polyribosomes.

19 edominantly cytoplasmic, and associates with polyribosomes.

20 mic lysates is predominantly associated with polyribosomes.

21 gag RNA nuclear export and association with polyribosomes.

22 n of MBP mRNAs associated with membrane-free polyribosomes.

23 nt gamma-globin chains prior to release from polyribosomes.

24 cruit and also to maintain maternal mRNAs on polyribosomes.

25 tment, Cp mRNA was primarily associated with polyribosomes.

26 tion of numerous transcripts and subsynaptic polyribosomes.

27 small ribosome subunits, monoribosomes, and polyribosomes.

28 mRNP particles that are not associated with polyribosomes.

29 to an increase in the amount of p27 mRNA in polyribosomes.

30 pected for proteins that are associated with polyribosomes.

31 istributed with 80 S ribosomal particles and polyribosomes.

32 liced and polyadenylylated, and localized to polyribosomes.

33 aining the 3' stem-loop from both nuclei and polyribosomes.

34 hylguanosine cap, and did not associate with polyribosomes.

35 involving increased association of mRNA with polyribosomes.

36 aptic mRNAs are transported as stably paused polyribosomes.

37 RNase through interaction with the enzyme in polyribosomes.

38 genous Dhh1 accompanies slowly translocating polyribosomes.

39 and (3) Herc5 is physically associated with polyribosomes.

40 inase C (PKC)-induced recruitment of mRNA to polyribosomes.

41 ch is incorporated into actively translating polyribosomes.

42 global protein synthesis and disassembly of polyribosomes.

43 tinal isoforms of IMPDH1 also associate with polyribosomes.

44 found to export and associate normally with polyribosomes.

45 tubule-bound mRNAs (MT-mRNAs) associate with polyribosomes.

46 The dynamics of polyribosome abundance were studied in gravistimulated m

47 We previously found that polyribosomes accumulate in dendritic spines of the adul

48 Here we optimize methods to analyze brain polyribosomes, allowing us to definitively demonstrate t

49 upled in vitro transcription/translation and polyribosome analysis demonstrated a strong association

50 Polyribosome analysis indicated that oxidized bases in m

51 for FMRP function, we examined their role in polyribosome and mRNA association.

52 specially on enlarged spines containing both polyribosomes and a spine apparatus.

53 grown under non-permissive conditions, lose polyribosomes and accumulate free 80S ribosomes.

54 s of evidence that JAKMIP1 is a component of polyribosomes and an RNP translational regulatory comple

55 his network is localized in dendrites, where polyribosomes and associated membranous elements are pos

56 ) Ferredoxin 1 (Fed-1) mRNA dissociates from polyribosomes and becomes destabilized when photosynthes

57 le promoter results in formation of half-mer polyribosomes and decreased Qsr1p levels on free 60S sub

58 with RNase A caused a coincident collapse of polyribosomes and each Upf protein into fractions contai

59 quantitatively associated with mRNA, both in polyribosomes and in free mRNPs.

60 component, and found that FMRP moved out of polyribosomes and into SGs subsequent to oxidative stres

61 a large portion of the mRNA dissociates from polyribosomes and is subsequently degraded.

62 rotein-coding IFNL4 mRNA are not loaded onto polyribosomes and lack a strong polyadenylation signal,

63 We report that Bfr1p associates with yeast polyribosomes and mRNP complexes even in the absence of

64 There were more polyribosomes and multivesicular bodies throughout the d

65 tem-loop of histone mRNA, is present in both polyribosomes and nuclei.

66 , thereby reducing p53 mRNA association with polyribosomes and p53 translation.

67 its Drosophila homolog, ATX2, assemble with polyribosomes and poly(A)-binding protein (PABP), a key

68 erely reduced the association of TbAGO1 with polyribosomes and RNAi-induced cleavage of mRNA.

69 r attention was paid to the integrity of the polyribosomes and rough endoplasmic reticulum.

70 inal domain blocked association of AGO1 with polyribosomes and severely affected mRNA cleavage.

71 cantly depleted in organelle content such as polyribosomes and showed abnormal (vesiculated) mitochon

72 ng detected Fyn mRNA specifically in soluble polyribosomes and soluble Fyn protein was only detected

73 scharge subsided beyond 10 s, FMRP levels in polyribosomes and stress granules did not return to basa

74 Furthermore, coincident polyribosomes and synapse enlargement might indicate spi

75 er 30 min of gravistimulation, the levels of polyribosomes and the amount of polyribosome-associated

76 stingly, these complexes are associated with polyribosomes and the endoplasmic reticulum.

77 n translation as it is found associated with polyribosomes and the rough endoplasmic reticulum.

78 y nondescript but contain massive numbers of polyribosomes, and (5) labeled degenerating hair cells.

79 dant cytoplasmic rough endoplasmic reticulum/polyribosomes, and accumulations of sub-plasmalemmal mic

80 4 cosediments with 40S ribosome subunits and polyribosomes, and its knockdown decreases translation.

81 at permissive temperatures, slows run-off of polyribosomes, and reduces binding to eEF1A.

82 her MAEL nor piRNA precursors associate with polyribosomes, and they may arise from an imbalance betw

83 of TbAGO1 is that a fraction sediments with polyribosomes, and this association is facilitated by an

84 rs even when most of the mRNA is retained on polyribosomes, and thus is likely an independent event r

85 leaves is stabilized by its association with polyribosomes, and/or by translation.

86 In mammalian neurons, polyribosomes are found not just in the cell body, but a

87 he levels 40 S, 60 S, and 80 S monosomes and polyribosomes are unaffected by the loss of PRMT3, but t

88 in cycloheximide-treated cells is present on polyribosomes as a result of continued synthesis and tra

89 H domains and RGG boxes that associates with polyribosomes as a ribonucleoprotein particle.

90 ing protein in yeast known to associate with polyribosomes as an mRNP component, although its biologi

91 sociate with both soluble and membrane-bound polyribosomes as an mRNP component.

92 NA was able to displace FXR1P and FXR2P from polyribosomes as it does for FMRP, and this displacement

93 ing protein that associates with translating polyribosomes as part of a large messenger ribonucleopro

94 luding thylakoid membranes, carboxysomes and polyribosomes, as well as phages, inside the congested c

95 m knockout mice did not manifest accelerated polyribosome assembly or protein synthesis as it occurs

96 in illuminated plants but in darkness is not polyribosome associated and is thus rapidly degraded by

97 ies detected by pCD41 are polyadenylated and polyribosome associated, suggesting that they may be act

98 miR-107 levels, leading to downregulation of polyribosome-associated MOR-1A in both Be(2)C cells and

99 he levels of polyribosomes and the amount of polyribosome-associated mRNA gradually increased over 24

100 s was accompanied by a transient decrease in polyribosome-associated mRNA.

101 nerve processes was used to screen synaptic polyribosome-associated mRNA.

102 ufficient to prevent complete translation of polyribosome-associated mRNAs.

103 FMRP is a polyribosome-associated neuronal RNA-binding protein, su

104 0 rapidly shifts existing TNFalpha mRNA from polyribosome-associated polysomes to monosomes.

105 Although FMRP has been characterized as a polyribosome-associated regulator of translation, less i

106 high-fat diet for 7 days showed increases in polyribosome-associated RNA and phosphorylation of S6K,

107 Longer incubations led to depletion of polyribosome-associated RNA, consistent with activation

108 FMRP is a polyribosome-associated RNA-binding protein that regulat

109 expression of multiple genes, is one of the polyribosome-associated transcripts.

110 f function, but the mutant proteins remained polyribosome-associated.

111 ny of the events exhibiting isoform-specific polyribosome association are highly conserved across mam

112 nucleus and cytoplasm, and further implicate polyribosome association as an essential component of Sc

113 iguous segments of GCN1 that lead to reduced polyribosome association by GCN1.GCN20 in living cells w

114 RNA specifically undergoes markedly enhanced polyribosome association in the hippocampus in response

115 ur before the decline in mRNA abundance, and polyribosome association is rapidly reversible if plants

116 Furthermore, polyribosome association is severely compromised for bot

117 We find that differences in polyribosome association may be explained, at least in p

118 l inhibitors, does not inhibit the efficient polyribosome association of a Puf5p target mRNA.

119 ites in the 3'UTR have a strong influence on polyribosome association of alternative mRNA isoforms.

120 t nuclear pre-mRNA processing influences the polyribosome association of alternative mRNA isoforms.

121 isease-causing mutation, D226N, disrupts the polyribosome association of at least one retinal IMPDH1

122 ly responsive to light, we characterized the polyribosome association of these mRNAs in the light and

123 9G8 also enhanced polyribosome association of unspliced RNA containing a C

124 The Top3beta-polyribosome association requires TDRD3, which directly

125 A abundance are associated with increases in polyribosome association that require both a functional

126 es 533 and 538 were required for normal FMRP polyribosome association whereas all four arginines play

127 the same binding site on RNA mediating their polyribosome association, and that they may be functiona

128 titutions blocked the normal light effect on polyribosome association, whereas both altered dark-indu

129 in mouse erythroblasts and investigated mRNA polyribosome association, which revealed deregulated tra

130 led to a loss of siRNAs but did not abolish polyribosome association.

131 nce for the important role of the RGG box in polyribosome association.

132 and Levy first reported their observation of polyribosomes at the base of spines, the prevailing view

133 ation or to the presence of half-mers, i.e., polyribosomes awaiting a 60S subunit, our data show that

134 multaneously produced a greater reduction in polyribosome binding by GCN1.GCN20 and a stronger decrea

135 is enhanced phosphorylation of at least six polyribosome binding proteins.

136 Among the polyribosome bound proteins are the p90rsk-activating ki

137 gy and gene expression profiles of total and polyribosome-bound mRNA genome wide.

138 lts demonstrate that Scp160p associates with polyribosome-bound mRNP complexes in vivo, implicating a

139 hat unfolded protein response (UPR)-elicited polyribosome breakdown resulted in the continued associa

140 he initiation stage of protein synthesis and polyribosome breakdown.

141 ng that FMRP in brain is not associated with polyribosomes, but is part of small ribonucleo-protein c

142 ction increased c-jun/betaGal mRNA levels in polyribosomes by 2.3-fold, which indicates that translat

143 we have found that Scp160p is released from polyribosomes by EDTA in the form of a large complex of>

144 ts suggest that the virion RNase is bound to polyribosomes by virtue of the reported association with

145 We further show that mRNA on polyribosomes can be targeted for degradation, although

146 ent and poly(A)- mRNA associate with smaller polyribosomes compared with cells with normal ribosome l

147 ions in AD and directly demonstrate that the polyribosome complex is adversely affected early in the

148 nstrate in neuroblastoma cells that the FMRP-polyribosome complexes are sensitive to puromycin, a dru

149 in enhanced recruitment of the HCV RNA into polyribosome complexes in vivo but only partially rescue

150 Accordingly, the HP RNA was retained within polyribosome complexes in vivo that were refractory to I

151 Similarly, polyribosome complexes remained stably positioned at spi

152 y of the Otx2(lambda) mRNA to form efficient polyribosome complexes was impaired.

153 bp in wild-type cells is not associated with polyribosome complexes.

154 nslocation of multifunction kinase p90rsk to polyribosomes; concomitantly, there is enhanced phosphor

155 onstrates that dFMRP does not associate with polyribosomes, consistent with their reported exclusion

156 ally, we find that IMPDH1 is associated with polyribosomes containing rhodopsin mRNA.

157 the majority of transcripts associated with polyribosomes decreased, resembling a general stress res

158 Analysis of liver polyribosomes demonstrated that the post-transcriptional

159 n of transcripts for vacuolar invertase with polyribosomes did not change over this period.

160 vivo but only partially rescued the RNA from polyribosome dissociation induced by IFN treatment.

161 et of the mRNAs showed dramatic dark-induced polyribosome dissociation, similar to Fed-1 mRNA, and al

162 and all of the mRNAs showed at least slight polyribosome dissociation.

163 red with eIF4GI depletion were excluded from polyribosomes due to the presence of multiple upstream o

164 Additional studies revealed that polyribosomes extracted from cytoplasm of wild-type viru

165 ly up-regulated and selectively recruited to polyribosomes following influenza virus infection.

166 y recruited maternal mRNAs were removed from polyribosomes following subsequent cleavage of eIF4G, in

167 ion of DHX33 protein levels markedly reduced polyribosome formation and caused the global inhibition

168 d RBV inhibit HCV IRES through prevention of polyribosome formation.

169 cDNA array analysis of the polyribosome fraction demonstrated that retinoic acid re

170 , the advantage of which is that hundreds of polyribosome fractionations can be performed simultaneou

171 significantly increased levels in the heavy polyribosome fractions following miRNA miR-30a-3p knockd

172 Upf2p shifted toward heavier polyribosome fractions in the absence of Upf1p and into

173 of each mutant protein into 30 S, 70 S, and polyribosome fractions in vivo.

174 nic stimulation, with a commensurate loss of polyribosomes from dendritic shafts at 2 hr posttetanus.

175 We isolated polyribosomes from Escherichia coli cells expressing GFP

176 Polyribosomes from fetal kidneys labeled in vitro with 1

177 termination and induces the dissociation of polyribosomes from mRNA.

178 rate incorporation of labeled amino acids by polyribosomes from neonatal kidneys declined sharply to

179 Association of eEF2 with polyribosomes, however, was unchanged upon expression of

180 Utilizing RiboTag mice and polyribosome immunoprecipitation, we performed endotheli

181 xCR1 mRNA was associated with polyribosomes in animal cells but not vegetal cells.

182 ere they mediate mRNA translation as part of polyribosomes in animals.

183 etion transcript variant was associated with polyribosomes in cells.

184 scripts for HMGR-FLAG remain associated with polyribosomes in dark-treated tissues.

185 ectron microscopy reconstructions to examine polyribosomes in dendrites when memory formation was blo

186 Although electron microscopy has identified polyribosomes in dendrites, in particular in postsynapti

187 We found that 4EGI-1 depleted polyribosomes in dendritic shafts and selectively preven

188 The presence of polyribosomes in dendritic spines suggests a potential i

189 ively enriched in actively translating large polyribosomes in IL-4-pretreated cells compared with cel

190 relatively stable when it is associated with polyribosomes in illuminated plants but in darkness is n

191 Each of the MIE transcripts associates with polyribosomes in infected cells and therefore contribute

192 ctron microscopy reconstructions to quantify polyribosomes in LA dendrites when consolidation was blo

193 ndicate that FMRP associates with functional polyribosomes in neurons.

194 The association of FMRP with polyribosomes in non-neural cell lines has previously su

195 mber of RNAs show increased association with polyribosomes in PDK-1-/- ES cells relative to PDK-1+/+

196 erential association of ATF5 mRNA with large polyribosomes in response to stress.

197 d to fractions lighter than those containing polyribosomes in the absence of Upf2p.

198 ic spine bases, (3) endoplasmic reticula and polyribosomes in the cytoplasm of dendritic shafts, and

199 Electron microscopy showed ribosomes/polyribosomes in the distal parts of axon tips and in as

200 RNAs containing this element dissociate from polyribosomes in the leaves of transgenic tobacco (Nicot

201 found that Fed-1 transcripts accumulated on polyribosomes in the light but were found primarily in n

202 o alter the association of Tmprss6 mRNA with polyribosomes in the liver of rats indicating a lack of

203 profiles revealed a significant reduction in polyribosomes in torpid animals, indicating that transla

204 ocampus, FMRP shifted into SGs and away from polyribosomes, in both hippocampi.

205 The percentage of spines containing polyribosomes increased from 12% +/- 4% after control st

206 Analysis of translating polyribosomes indicated that hsp90 interacts with nascen

207 nown to differentially disrupt components of polyribosomes indicated that Pabp may require contact wi

208 ation status and they remain associated with polyribosomes, indicating inhibition at a postinitiation

209 o attenuation of IL-12 mRNA association with polyribosomes inhibiting translation while IL-10 mRNA as

210 rate that the association of FMRP with brain polyribosomes is abrogated by competition with the FMRP

211 Histone mRNA from polyribosomes is immunoprecipitated with anti-SLBP.

212 The assembly of ATX2 with polyribosomes is mediated independently by two distinct

213 ethod for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed ce

214 735A mutant bound siRNAs and associated with polyribosomes, it displayed a severe defect in the cleav

215 tion is initiated prematurely in perinuclear polyribosomes, leading to overproduction of receptors bu

216 elective RNA-binding protein associated with polyribosomes, leading to the hypothesis that FMRP may b

217 at FMRP interaction with RNA and translating polyribosomes leads to synapse loss.

218 The transient decrease in polyribosome levels was accompanied by a transient decre

219 rocess coupled to photosynthesis affects the polyribosome loading of a subset of cytoplasmic mRNAs.

220 RBV also blocked polyribosome loading of HCV-IRES mRNA through the inhibi

221 portant for Fed-1 mRNA stability rather than polyribosome loading.

222 Further treatment of polyribosome lysates with agents known to differentially

223 nslation with a significant reduction in the polyribosome/monoribosome ratio for Pdx1 mRNA.

224 Polyribosomes, mRNA, and other elements of translational

225 data indicate that association of FMRP with polyribosomes must be functionally important and imply t

226 Although HAC1u mRNA is associated with polyribosomes, no detectable Hac1pu is produced unless t

227 We found that palmitate acutely increases polyribosome occupancy of total RNA, consistent with an

228 calreticulin and calmodulin transcripts into polyribosomes occurred predominantly in the lower pulvin

229 In parallel studies, mRNAs from polyribosomes of fragile X cells were used to probe micr

230 the interaction between TDP-43 and RACK1 on polyribosomes of neuroblastoma cells with mis-localizati

231 Immunoaffinity purification of polyribosomes (polysomes) from crude leaf extracts of Ar

232 nation promoted adjustments in the levels of polyribosomes (polysomes) that were highly coordinated w

233 ere, we determined that CSP1 associates with polyribosomes (polysomes) via an RNA-mediated interactio

234 The concept of a distinct polyribosome-populated domain, distributed intermittentl

235 Polyribosome profile analysis confirmed that elevated ma

236 We sought to address this issue through polyribosome profile analysis of islets from mice fed 16

237 plasmic abundance, 251 mRNAs had an abnormal polyribosome profile in the absence of FMRP.

238 dom34delta mutants display an altered polyribosome profile that is rescued by expression of RP

239 There was no change in the shape of the polyribosome profile with increasing age, but before bir

240 on (TMA7), translation fidelity (TMA20), and polyribosome profiles (TMA7, TMA19, and TMA20).

241 In dbp2Delta cells, polyribosome profiles are deficient in free 60S subunits

242 cting shifts in individual mRNA abundance in polyribosome profiles following miRNA knockdown via siRN

243 had no effect on either the abundance or the polyribosome profiles of endogenous histone H1 or transg

244 s had little effect on cell growth or on the polyribosome profiles.

245 ation requires translation, we also examined polyribosome profiles.

246 by methionine incorporation and analysis of polyribosome profiles.

247 By polyribosome profiling of tumor tissues and human breast

248 We developed a brain polyribosome-programmed translation system, revealing th

249 ion of the RNAi machinery and target mRNA on polyribosomes promotes an efficient RNAi response.

250 d it is straightforward and does not require polyribosome purification or in vitro RNA footprinting.

251 protein synthesis, accumulation of half-mer polyribosomes, reduced levels of 60S ribosomal subunits

252 s and the appearance of new complexes in the polyribosome region of the gradient.

253 ults in increased loading of H2AFX mRNA onto polyribosomes, resulting in increased expression of H2A.

254 al ratio of ribosomes to mRNA as revealed by polyribosome sedimentation analysis.

255 Polyribosome sedimentation velocity centrifugation can b

256 stribution of key cellular resources such as polyribosomes, smooth endoplasmic reticulum, and synapti

257 rane system comprising the nuclear envelope, polyribosome-studded peripheral sheets, and a polygonal

258 of interferon regulatory factor-1 mRNA with polyribosomes, suggesting iron was not essential for int

259 a 7.6-kilobase RNA that was associated with polyribosomes, suggesting it is translated.

260 ncorporation of c-fos mRNA into the heaviest polyribosomes, suggesting redox regulation of c-fos mRNA

261 en was associated with spine apparati and/or polyribosomes, suggesting that estrogen might act locall

262 IMPDH1) and IMPDH type 2 are associated with polyribosomes, suggesting that these housekeeping protei

263 RNA assembles into 60 S ribosomes which form polyribosomes, suggesting that they function in protein

264 rtion of dendritic spine synapses containing polyribosomes than did the cortices of wild-type mice, c

265 Repressed mRNAs are associated with polyribosomes that are engaged in translation elongation

266 This revealed two major pools of polyribosomes that were upregulated during memory format

267 e miRNAs that were tested cofractionate with polyribosomes, the sites of active translation.

268 r1p disrupts the interaction of Scp160p with polyribosomes, thereby demonstrating that the relationsh

269 Unlike normal polyribosomes, these complexes were resistant to breakdo

270 overall translation and that its binding to polyribosomes through RACK1 may promote, under condition

271 rotein upon synaptic signaling using stalled polyribosomes to bypass the rate-limiting step of transl

272 sed from stress granules and associates with polyribosomes to increase protein synthesis in a CAP-ind

273 a subtle yet significant shift from soluble polyribosomes to soluble mRNPs for at least two of these

274 min of gravistimulation, the amount of large polyribosomes transiently decreased.

275 s suggest that the association of Upf2p with polyribosomes typically found in a wild-type strain depe

276 pletion of PTC-containing mRNA isoforms from polyribosomes, underscoring the functional relationship

277 that normal FMRP associates with elongating polyribosomes via large mRNP particles.

278 ranslation while IL-10 mRNA association with polyribosomes was not affected.

279 calreticulin and calmodulin transcripts with polyribosomes was seen predominantly in the lower one-ha

280 ies showing that VHS-RNase degrades mRNAs in polyribosomes, we constructed a mutant in which NES was

281 Although many MT-mRNAs associate with polyribosomes, we find that active translation is not re

282 Polyribosomes were associated with the spine apparatus u

283 IIalpha) mRNA levels in actively translating polyribosomes were dysregulated in synaptoneurosomes fro

284 Postsynaptic densities on spines containing polyribosomes were larger after tetanic stimulation.

285 lated 9G8 was present in monosomes and small polyribosomes, whereas soluble fractions contained only

286 rilipin A mRNAs were efficiently loaded with polyribosomes whether or not fatty acids were added to t

287 quire contact with some RNA component of the polyribosome, which could be either non-poly(A)-rich seq

288 proteins from several animals associate with polyribosomes, which are units of mRNA translation, wher

289 As containing 5' UTR GRSF-1 binding sites to polyribosomes, which is mediated through interactions wi

290 ed FMRP associated with actively translating polyribosomes while a fraction of phosphorylated FMRP is

291 n and greater association of the M mRNA with polyribosomes, while general translation was unaffected.

292 represent the quantal product of one or more polyribosomes, while inter-flash intervals appear random

293 Treatment of the polyribosomes with micrococcal nuclease prior to salt ex

294 -10% of the SLBP could be extracted from the polyribosomes with salt.

295 90% of the SLBP in the cell is found in the polyribosomes with the remaining SLBP localized to the n

296 reticulin and calmodulin were recruited into polyribosomes within 15 min of gravistimulation.

297 d that iLRE-containing mRNAs dissociate from polyribosomes within 20 min after plants are transferred

298 Ultrastructural analysis revealed polyribosomes within growth cones that colocalized with

299 ins and miRNAs biochemically cosediment with polyribosomes, yet another fraction paradoxically accumu

300 cated in the cytosol, and is associated with polyribosomes, yet does not produce protein, indicating