ライフサイエンスコーパス: polysialic acid

1 ion, which is released upon interaction with polysialic acid.

2 omote neurite outgrowth similarly to natural polysialic acid.

3 cking to secondary lymphatic organs, carries polysialic acid.

4 , which expresses long-chain a(2-->8)-linked polysialic acid.

5 f N-glycans substituted with short chains of polysialic acid.

6 scription 3; these processes were blocked by polysialic acid.

7 sferase catalyzed the synthesis of alpha-2,8-polysialic acid.

8 orted G2(+) left-handed helix of alpha-(2,8)-polysialic acid.

9 eased in vivo and in vitro in the absence of polysialic acid.

10 g and distinguishing between different oligo/polysialic acids.

11 antibody response against alpha-(2,8)-linked polysialic acids.

12 hesis and catabolism of microbial sialic and polysialic acids.

13 Polysialic acid, a homopolymer of alpha2,8-linked sialic

14 the transfer of neuNAc from CMP-neuNAc to a polysialic acid acceptor is catalyzed by a complex with

15 ive fashion when initiated with an alpha-2,8-polysialic acid acceptor.

16 to be mediated at least in part by increased polysialic acid addition to neural cell adhesion molecul

17 Loss of polysialic acid affected both tangential and radial migr

18 These results indicate that polysialic acid and mucin type O-glycans on NCAM differe

19 eningitidis serogroup B, (alpha2-->8)-linked polysialic acid and the capsules of other meningococcal

20 an rhabdomyosarcoma cells, which overexpress polysialic acid, and C2C12 cells.

21 , isogenic serogroups B [(alpha2-->8)-linked polysialic acid] and C [(alpha2-->9)-linked polysialic a

22 Anti-oligo/polysialic acid antibodies have DP-dependent antigenic s

23 Naturally occurring polysialic acids are not viable candidates because they

24 erase that catalyzes the formation alpha-2,9-polysialic acid as a soluble enzyme.

25 d from CMP-sialic acid to a growing chain of polysialic acid at the nonreducing end.

26 Polysialic acid attached to the neural cell adhesion mol

27 sis of mutants that accumulate intracellular polysialic acid because of export defects (kpsM and kpsS

28 nt virulence factor in these diseases is the polysialic acid capsular polysaccharide (K1 antigen), wh

29 is group C are based on its alpha-2,9-linked polysialic acid capsular polysaccharide.

30 pid attached to the reducing terminus of the polysialic acid capsular polysaccharides from E. coli K1

31 ans and domestic animals; in this strain the polysialic acid capsule (K1 antigen) functions by inhibi

32 ecific pathogen Escherichia coli K1 uses its polysialic acid capsule as a molecular mimic to engage S

33 s confirm the intrinsic relationship between polysialic acid capsule biosynthesis and lipooligosaccha

34 involved in CMP-N-acetylneuraminic acid and polysialic acid capsule biosynthesis, and in ctrA the fi

35 kpsM, allowing the expression of an alpha2,8 polysialic acid capsule in E. coli.

36 esults indicate that the (alpha2-->8)-linked polysialic acid capsule modifies the interaction of meni

37 ns 7 or 9 of the sialic acid residues in the polysialic acid capsule of Escherichia coli K1 is cataly

38 utilizes ATP to effect translocation of the polysialic acid capsule of Escherichia coli K1.

39 es, the influence of the alpha2-->8) -linked polysialic acid capsule on the interaction of N. meningi

40 Opa, Opc, glycolipid GgO4-binding adhesins, polysialic acid capsule or a particular lipooligosacchar

41 herichia coli K1 synthesizes and assembles a polysialic acid capsule virulence factor on the external

42 ene cluster required for the biosynthesis of polysialic acid capsule was mapped to a location immedia

43 rom an isogenic bacterial mutant lacking the polysialic acid capsule.

44 lation or acetylation of the alpha2-9-linked polysialic acid capsule.

45 The removal of polysialic acid caused several distinct abnormalities, i

46 ains its apparent high affinity for a longer polysialic acid chain by recognizing every three sialic

47 ct with the NCAM acidic patch or the growing polysialic acid chain.

48 he unit catalyzing the extension of existing polysialic acid chains does not differ significantly fro

49 ively non-immunogenic molecular mimic of the polysialic acid chains found in high concentrations on n

50 enzyme itself is modified by alpha2,8-linked polysialic acid chains in vivo.

51 erases bind to the FN1 of NCAM to polymerize polysialic acid chains on appropriately presented glycan

52 However, elongation of preexisting polysialic acid chains only requires expression of neuS.

53 opical methods demonstrated that full-length polysialic acid chains were synthesized but not exported

54 ed polysialyltransferases suggest that their polysialic acid chains, like those of NCAM, may modulate

55 f the individual sialic acid residues of the polysialic acid chains.

56 The virulent E. coli strain had a polysialic acid-containing capsule, whereas the nonvirul

57 glycosphingolipids characterized by mono- or polysialic acid-containing oligosaccharides linked throu

58 lysialyltransferase, NeuS, cannot synthesize polysialic acid de novo without other products of the ge

59 ng the entire K1 gene cluster can synthesize polysialic acid de novo.

60 Consistent with these findings, polysialic acid-deficient mice exhibited increased expre

61 s a unique preference for longer polymers of polysialic acid (DP >10), yet the mechanism of recogniti

62 tial targeting of polysialyltransferases and polysialic acid engineering are promising strategies to

63 Polysialic acid exhibits a highly regulated expression p

64 t least four of these genes are required for polysialic acid export.

65 This polysialic acid expressed on the surface of N. meningiti

66 xamined how unnatural sialic acids can alter polysialic acid expression and influence the adhesive pr

67 In a previous study, we showed that polysialic acid facilitates astrocytic tumor invasion an

68 Some SEZ cells expressed the polysialic acid form of neural cell adhesion molecule (P

69 Here, we describe an analysis of capsular polysialic acid form variation in Escherichia coli K1, d

70 oducts required for de novo synthesis of the polysialic acid from CMP-NeuNAc in K1 E. coli.

71 ase recognition, but shifted the addition of polysialic acid from the N-glycans modifying the adjacen

72 Removal of polysialic acid from the surface of dendritic cells or b

73 ed using chemically modified N-propionylated polysialic acid, from Escherichia coli K1 polysaccharide

74 It is currently unclear how polysialic acid functions in different processes of neur

75 Polysialic acid has been detected in multiple sclerosis

76 it can form high-molecular-weight alpha-2,9-polysialic acid in a nonprocessive fashion when initiate

77 By contrast, forced expression of polysialic acid in early differentiation stages reduces

78 of mouse adult brain, however, suggests that polysialic acid in the hippocampal formation is synthesi

79 and STX are polysialyltransferases that form polysialic acid in the neural cell adhesion molecule (N-

80 and STX are polysialyltransferases that form polysialic acid in the neural cell adhesion molecule (NC

81 t sufficient to support de novo synthesis of polysialic acid in vitro.

82 ested in developing structural surrogates of polysialic acids in an effort to overcome these limitati

83 Polysialic acid is a developmentally regulated, anti-adh

84 Polysialic acid is a developmentally regulated, anti-adh

85 Polysialic acid is a glycan modification of the neural c

86 Polysialic acid is a linear homopolymer of alpha2-8-link

87 Polysialic acid is a unique carbohydrate polymer specifi

88 Polysialic acid is an anti-adhesive glycan that modifies

89 Polysialic acid is an anti-adhesive protein modification

90 Polysialic acid is an oncofetal glycopolymer, added to t

91 accharides and that the vast majority of the polysialic acid is found on the oligosaccharide modifyin

92 Polysialic acid is primarily attached to N-glycans of NC

93 of the Escherichia coli K1 group 2 capsular polysialic acid (K1 antigen) occur within a protected su

94 , we show that Gata1-KO(DC) DCs have reduced polysialic acid levels on their surface, which is a know

95 polysialic acid] and C [(alpha2-->9)-linked polysialic acid] meningococcal isolates from an outbreak

96 Measurements with the polysialic-acid-modified form of NCAM reveal that, at ph

97 we demonstrate that selectively cleaving the polysialic acid moiety, using the bacteriophage-derived

98 We identified the polysialic acid molecule (PSA) as a mediator of the diet

99 abile glycans such as heavily sialylated and polysialic acid N-glycans, which are difficult to detect

100 ursor (NRP) cell that expresses E-NCAM (high polysialic-acid NCAM) and is morphologically distinct fr

101 Polysialic acid negatively regulates cell adhesion, is r

102 erotype are encapsulated with the alpha(2-8)-polysialic acid NeuNAc(alpha2-8), common to several bact

103 tochemistry for bromodeoxyuridine (BrdU) and polysialic acid-neural cell adhesion molecule (PSA-NCAM)

104 The up- and downregulation of polysialic acid-neural cell adhesion molecule (PSA-NCAM)

105 s, stathmin immunoreactivity was observed in polysialic acid-neural cell adhesion molecule-positive m

106 alyltransferase (PST) that forms the group C polysialic acid (NmC PST) is located in the cytoplasmic

107 al importance of O-acetylation, no sialic or polysialic acid O-acetyltransferase has been identified

108 es of di- and trisaccharide fragments of the polysialic acid O-antigen capsular polysaccharide (CPS)

109 ST8SiaIV to determine the effects of loss of polysialic acid on brain development.

110 The presence of polysialic acid on NCAM has been shown to modulate cell-

111 of PST and STX more efficiently synthesized polysialic acid on NCAM than PST or STX alone.

112 hich these two enzymes form large amounts of polysialic acid on NCAM were heretofore unknown.

113 the normal expression patterns of EphA4 and polysialic acid on NCAM, which may contribute to the pat

114 he sialyltransferase gene family, synthesize polysialic acid on NCAM.

115 ptors but differ in the efficiency of adding polysialic acid on NCAM.

116 PST and STX were found to add polysialic acid on NCAM.Fc molecules sialylated by alpha

117 ormal expression levels of EphA4, EphB1, and polysialic acid on neural cell adhesion molecule, three

118 ase (STX) gene expression and an increase in polysialic acid on neural cell adhesion molecule.

119 First, we found that ST8Sia III can form polysialic acid on the enzyme itself (autopolysialylatio

120 Polysialic acid on the neural cell adhesion molecule (NC

121 for complex N-glycans showed the presence of polysialic acid on the neural cell adhesion molecule.

122 a II, and ST8Sia III all add oligosialic and polysialic acid on various sialylated N-acetyllactosamin

123 utional, and functional diversities of oligo/polysialic acids (OSA/PSA) that exist in organisms rangi

124 Cell surface polysialic acid plays roles in cell adhesion and differe

125 t nucleus of the solitary tract has abundant polysialic acid (polySia) and is a major site of integra

126 STATEMENT The beneficial or adverse role of polysialic acid (polySia) in myelin repair is a long-sta

127 Polysialic acid (polySia) is a large glycan polymer that

128 Polysialic acid (polySia) is a large glycan with restric

129 Polysialic acid (polySia) is known for its important rol

130 anNBut) with a goal to achieve modulation of polysialic acid (polySia) on neural cell adhesion molecu

131 and co-workers demonstrated the presence of polysialic acid (polySia) on sea urchin sperm.

132 This linkage is prominent in polysialic acid (polySia), a molecule with critical role

133 econdary human lymphoid organs, its product, polysialic acid (polySia), has been largely overlooked b

134 xpression of the large extracellular glycan, polysialic acid (polySia), is restricted in the adult, t

135 ar homo-polymers, with its most complex form polysialic acid (polySia).

136 1, a function that relies on the presence of polysialic acid (polySia).

137 t encode transport proteins (yihN and yihP), polysialic acid production (gutM and gutQ), CP4-57 proph

138 Polysialic acid (PSA) and its major protein carrier, the

139 sent study was to evaluate the expression of polysialic acid (PSA) and the cell adhesion molecule L1

140 membrane-membrane apposition based solely on polysialic acid (PSA) biophysical properties.

141 Polysialic acid (PSA) capsules are cell-associated homop

142 A role for the polysialic acid (PSA) component of PSA-NCAM, which is kn

143 Enzymatic removal of polysialic acid (PSA) from nerve and muscle during norma

144 Polysialic acid (PSA) fulfills several criteria for a mo

145 aspects of human biology, the expression of polysialic acid (PSA) in human tissues is thought to be

146 The highly negatively charged polysialic acid (PSA) is a carbohydrate predominantly ca

147 Polysialic acid (PSA) is a developmentally regulated car

148 Polysialic acid (PSA) is a homopolymeric glycan that pla

149 Polysialic acid (PSA) is a large negatively charged glyc

150 Polysialic acid (PSA) is a linear homopolymer of alpha-2

151 Polysialic acid (PSA) is a post-translational protein mo

152 Polysialic acid (PSA) is a unique linear homopolymer of

153 In vertebrates, polysialic acid (PSA) is typically added to the neural c

154 The polysialic acid (PSA) modification of the neural cell ad

155 The polysialic acid (PSA) moiety of NCAM can serve as a nega

156 The polysialic acid (PSA) moiety of the neural cell adhesion

157 to be reflected most sensitively in reduced polysialic acid (PSA) on neural cell adhesion molecules.

158 the repulsion also depends on the amount of polysialic acid (PSA) on the membranes, and the PSA-depe

159 developmentally regulated marker 2,8-linked polysialic acid (PSA) regulate viral transport and tropi

160 anoylmannosamine are effective inhibitors of polysialic acid (PSA) synthesis in stably transfected He

161 ot appear to alter sensory projections, when polysialic acid (PSA) was enzymatically removed from NCA

162 sion molecule (NCAM) is the major carrier of polysialic acid (PSA) which modulates NCAM functions of

163 of the spinal cord expresses high levels of polysialic acid (PSA), a cell surface carbohydrate known

164 Polysialic acid (PSA), a homopolymer attached to the neu

165 Polysialic acid (PSA), a large cell-surface carbohydrate

166 e is chemically identical to an autoantigen, polysialic acid (PSA), and is a poor immunogen, even whe

167 B and of Escherichia coli K1, alpha(2 --> 8) polysialic acid (PSA), is unusual, because when injected

168 Here we show that polysialic acid (PSA), presented by the neural cell adhe

169 The present study used polysialic acid (PSA)-deficient and NCAM mutant mice to

170 f the NCAM-180 isoform that in brain carries polysialic acid (PSA).

171 , and that this defect reflects loss of NCAM polysialic acid (PSA).

172 he predominant carrier of the unusual glycan polysialic acid (PSA).

173 nmodified MBPS or chemically identical human polysialic acid (PSA).

174 i K1 [which, like NMGB, is alpha(2-8)-linked polysialic acid (PSA)] and bound to EV36, a nonpathogeni

175 d with antibodies to N-CAM and antibodies to polysialic acid (PSA-N-CAM), which is present on N-CAM a

176 These results indicate that polysialic acid regulates cell migration and differentia

177 nt results suggest that de novo synthesis of polysialic acid requires coexpression of four genes from

178 th vomeronasal nerves (VNN) that express the polysialic acid-rich form of the neural cell adhesion mo

179 es strongly suggest that the N-propionylated polysialic acid-rPorB conjugate is an excellent vaccine

180 Expression of the (alpha2-->8)-linked polysialic acid serogroup B capsule was essential for me

181 The relaxivity of the gadolinium polysialic acid species formed in vitro was 97.8 mM/sec

182 ole-mount tecta of embryos pretreated with a polysialic acid-specific degrading enzyme, endoneuramini

183 tform for the rational design of alpha-(2,8)-polysialic acid surrogates.

184 Strikingly, inhibition of polysialic acid synthesis by antisense DNA approach indu

185 rization of full-length proteins involved in polysialic acid synthesis in E. coli K1, as well as hete

186 Sia II and ST8Sia IV, play dominant roles in polysialic acid synthesis on NCAM.

187 ividual and combined roles of PST and STX in polysialic acid synthesis, in the present study we asked

188 However, polysialic acid synthesized by PST appears to be a bette

189 The results also demonstrated that polysialic acid synthesized by PST is larger than that s

190 . coli strain having abundant outer membrane polysialic acid, targeted deletion of the polysialyltran

191 N-glycans containing more, and thus longer, polysialic acid than when the enzymes were used individu

192 hic molecule and can be elongated to produce polysialic acid that is reactive with group C-specific a

193 immune response against alpha(2-->8)-linked polysialic acid, the capsular polysaccharide of Group B

194 and concurrent translocation of the capsular polysialic acid through sites of inner and outer membran

195 d, neither ST8Sia II nor ST8Sia IV could add polysialic acid to a polysialylated antenna of NCAM N-gl

196 and replacing the alpha-helix or QVQ shifts polysialic acid to FN1 O-glycans in full-length NCAM.

197 SA-NCAM show heterophilic adhesion involving polysialic acid to heparan sulfate proteoglycan and agri

198 The addition of polysialic acid to N- and/or O-linked glycans, referred

199 aused by: 1) the ability of ST8Sia IV to add polysialic acid to oligosialic acid formed by ST8Sia II,

200 Furthermore, ST8Sia IV was able to add polysialic acid to oligosialylated oligosaccharides and

201 findings indicate that the unique ability of polysialic acid to regulate different types of cell inte

202 on in brain microglia and engages endogenous polysialic acid to suppress inflammation.

203 The addition of alpha2,8-polysialic acid to the N-glycans of the neural cell adhe

204 By contrast, ST8Sia II added little polysialic acid to the same acceptors.

205 region whose gene products are required for polysialic acid transport and because capsule production

206 e kps locus for biosynthesis of the capsular polysialic acid virulence factor in Escherichia coli K1

207 On this basis, the role of polysialic acid was analyzed with respect to both trajec

208 nas in N-glycans attached to NCAM, even when polysialic acid was attached to at least one of the othe

209 We found that larger polysialic acid was formed on the enzymes themselves (au

210 Polysialic acid was formed well on Lec4 and Lec13 cells,

211 Because so few proteins carry polysialic acid, we hypothesized that polysialylation is

212 nd gadolinium trichloride in the presence of polysialic acid were also performed.

213 Polysialic acid, which is synthesized by two polysialylt

214 ct of E. coli K92 catalyzes the synthesis of polysialic acid with alpha2,9- and alpha2,8-linkages in

215 number of mammalian proteins are modified by polysialic acid, with the neural cell adhesion molecule