ライフサイエンスコーパス: polysome

1 esis as the nascent proteins emerge from the polysome.

2 ctors may be constitutively localised to the polysome.

3 y and the association of the transcript with polysomes.

4 esent in all subpopulations of ER-associated polysomes.

5 se in the amount of grk mRNA associated with polysomes.

6 uces association of the UME6 transcript with polysomes.

7 s with the return of the stabilized mRNAs to polysomes.

8 3 translation and TP53 mRNA association with polysomes.

9 mRNAs into the active translation sites, the polysomes.

10 a2(I) mRNA is unrestrictedly loaded onto the polysomes.

11 p1 mutant for membrane-bound, but not total, polysomes.

12 ncreasing the loading of CBP/p300 mRNAs onto polysomes.

13 es with 40S ribosomal subunits and also with polysomes.

14 and it associates with mRNAs assembled into polysomes.

15 existing DHX33 mRNAs to actively translating polysomes.

16 of both PHO1;2 and cis-NATPHO1;2 toward the polysomes.

17 and the loading of CBP/p300 transcripts onto polysomes.

18 nally, IGFBP3 mRNA was found enriched to the polysomes.

19 onosomes and increased their target mRNAs in polysomes.

20 , of mRNAs encoding particular proteins from polysomes.

21 he cell coincident with their depletion from polysomes.

22 the tool to isolate coupled mitochondria and polysomes.

23 ap-dependent manner, were shifted to lighter polysomes.

24 te translation by actively recruiting RHA to polysomes.

25 lasm between the cytosol and the translating polysomes.

26 is present on many proteins that form active polysomes.

27 rotein synthesis rate and formation of large polysomes.

28 all of which are localized by membrane-bound polysomes.

29 ction motifs decrease LARP4 association with polysomes.

30 associated with cytosolic 80S ribosomes and polysomes.

31 transcript was present in RNA isolated from polysomes.

32 , and there was marked depletion of HuR from polysomes.

33 are detected in both ribonucleoproteins and polysomes.

34 PCBP 1 and 2, also cofractionated with viral polysomes.

35 ns uL4 or uL22 are still capable of entering polysomes.

36 cay (NMD), even though they were detected in polysomes.

37 ycolysis decreases TNF mRNA association with polysomes.

38 et of oxidative stress-responsive mRNAs into polysomes.

39 mammalian cells are in turn associated with polysomes.

40 vative, the chromatoid body, as well as with polysomes [9].

41 in vitro, ribosome transit rates slowed, and polysomes accumulated in intact cells, suggesting defect

42 oping multicolor probes, we showed that most polysomes act independently; however, a small fraction (

43 subunits in sucrose gradients and repopulate polysomes after a short starvation-induced translational

44 ADH2 mRNA rapidly dissociated from polysomes after glucose repletion, and sequences bound b

45 This heightened association of miRNA with polysomes also elicits reduced degradation of target mRN

46 Polysome analyses and in vitro reconstitution of initiat

47 tive reverse transcriptase PCR (qRT-PCR) and polysome analyses were performed.

48 Polysome analysis indicated that EF-P functions in trans

49 A combined eRIP and polysome analysis of early stage differentiation of hESC

50 lational profiling by microarray analysis of polysome and monosome associated mRNAs in wild-type and

51 ighly hypoxia-induced mRNAs are recruited to polysomes and actively translated, whereas other cellula

52 . cerevisiae resulted in the accumulation of polysomes and an increase in ribosomal transit times.

53 Cul4B is present on the polysomes and colocalizes with TTP to exosomes and proce

54 e dynamics of messenger RNA association with polysomes and compared the transcriptome with the transl

55 l Ccr4-Not complex in yeast, associates with polysomes and contributes to the negative regulation of

56 levels of actb mRNA associated with synaptic polysomes and diminished levels of synaptic actb protein

57 liced c-myb transcripts were associated with polysomes and encoded a series of c-Myb proteins with id

58 the SMN mRNA from heavy polysomes to lighter polysomes and monosomes, suggesting that Gemin5 function

59 ssembled ribosomes exist in two populations: polysomes and monosomes.

60 correlated with a global reduction in heavy polysomes and overall repression of translation.

61 Depletion of NAC from polysomes and re-localisation to protein aggregates is o

62 icient in loading uORF-containing mRNAs onto polysomes and stimulates translation in protoplasts, and

63 rent supra-ribosomal building blocks forming polysomes and suggest the presence of unexplored transla

64 In normal conditions, 70S-polysomes and the chromosomal DNA segregate, while 30S a

65 tively translating mRNAs are associated with polysomes and the newly synthesized peptide chains are c

66 ion influenced recruitment of mRNAs to heavy polysomes and translation of subsets of genes.

67 in annotated 5'-UTRs were overrepresented in polysomes and were as stable as canonical mRNA isoforms.

68 s are subject to differential recruitment to polysomes, and expose the importance of selective mRNA t

69 Rather, they are found on polysomes, and genetic analysis indicates that translati

70 ssion, promotes cap-dependent translation in polysomes, and reduces the anti-proliferative effect of

71 messenger ribonucleoprotein for loading onto polysomes, and reduction of Cul4B expression shunts the

72 Nonmuscle myosin copurifies with polysomes, and there is a subset of polysomes involved i

73 rmore, phosphorylated eIF4E relocates to the polysomes, and this contributes to changes in the transl

74 m a stable complex with the 70S ribosome and polysomes, and we demonstrate the proximity in vivo of r

75 iation of the mRNA with actively translating polysomes; and de novo MMP-9 protein synthesis were obta

76 n kinesin-3 and dynein-driven EEs, where the polysomes appeared to translate EE-associated mRNA into

77 ree 40S subunits are increased, and half-mer polysomes are accumulated.

78 We also observe that DYNC1H1 polysomes are actively transported by motors, which may

79 Here we show that polysomes are primarily formed by three distinct classes

80 ort and the distribution of the UL84 mRNA on polysomes are unaffected.

81 multaneously translate the same mRNA strand (polysomes) are represented as freely jointed chains of h

82 on microscopy to image ex-vivo-derived human polysomes as a source of actively translating ribosomes.

83 Finally, some DDX6 was associated with polysomes, as previously reported in yeast.

84 ll organization of mRNA and ribosomes within polysomes, as well as the possible role of this organiza

85 Chloroplast polysome assembly is reduced in csp41b-1/csp41a-1* mutan

86 crease in mRNA levels as well as a defect in polysome assembly that was independent of mRNA abundance

87 n about how proteins emerging from different polysomes associate to form hetero-oligomeric channels.

88 maydis, Higuchi et al. now demonstrate that polysomes associate with early endosomes that undergo ki

89 Collectively, our results indicate that polysomes associate with moving EEs and that "off- and r

90 at the mRNA encoding plexin A remains highly polysome associated during stress and escapes degradatio

91 ticles lacking srRNA 4 and reduced levels of polysome associated ribosomes.

92 NA targeted mRNAs are invariably found to be polysome associated; observations that appear to be at f

93 ARF caused a dramatic reduction in polysome-associated DHX33 mRNAs, while Ras(V12) led to a

94 Although psbA and rbcL mRNAs are normally polysome-associated in the mutant, petD-containing RNAs

95 Genome-wide analysis of cytosolic and polysome-associated messenger RNA (mRNA) revealed not on

96 tment did not change overall MOR transcript, polysome-associated mRNA declined in a let-7-dependent m

97 In contrast, the protein expression and polysome-associated mRNA levels of both genes were incre

98 combinatorial biotin transfer system, where polysome-associated mRNAs are selectively recovered from

99 logy clustering showed that the functions of polysome-associated transcripts differed between dormant

100 The changes in polysome-associated tRNA levels reflect the codon usage

101 anged following viral infection, whereas the polysome-associated tRNA population changes dramatically

102 delineated how a target mRNA passes through polysome association and Ago2 interaction steps on rough

103 Polysome association and detection of a low abundant 13.

104 and eIF4E2 are elucidated by observing their polysome association and the status of mammalian target

105 This novel combination results in increased polysome association of mda-7/IL-24 mRNA, activation of

106 Large differences in polysome association of miRNA target RNAs are found betw

107 We next observed that polysome association of nad7 mature mRNA is specifically

108 as promoted by UPF1 depletion, which induced polysome association of PTC-containing mRNAs, increased

109 riptional activity, and had no effect on the polysome association profile of HIF-1alpha.

110 Reduced polysome association was observed for additional key reg

111 exes from target mRNAs, leading to increased polysome association, translation, and stability of miRN

112 und mRNAs, we found that La binds 34% of the polysome bound mRNAs and regulates the expression of a s

113 Changes in polysome-bound mRNA (translatome) are correlated closely

114 ling of the rpl4d mutant showed reduction in polysome-bound mRNA compared with wild-type, but no sign

115 RNA-sequencing after isolation of polysome-bound mRNAs allows for cell-type-specific, geno

116 a in the cytoplasm by microarray analysis of polysome-bound mRNAs, we found that La binds 34% of the

117 is influenced by ionizing radiation, we used polysome-bound RNA to generate gene translation profiles

118 impact on the association of HCMV mRNAs with polysomes but significantly diminished the translation e

119 so determines the specificity of neocortical polysomes by defining their combinatorial composition of

120 nt of elongation, TOP mRNAs are recruited on polysomes causing a relative increase in the synthesis o

121 pholipase1 (Lypla1)--selectively entered the polysome compartment.

122 on and are a result of its direct binding to polysomes, complex formation with cellular RNAs (which i

123 lyzing mRNAs that dynamically associate with polysome complexes as neocortical development progresses

124 a-up-regulated gene transcripts increased in polysome complexes during the stress, but the number of

125 f of the cellular mRNAs were restricted from polysome complexes during the stress, with little or no

126 Ribosomal protein specificity within polysome complexes is regulated by the arrival of in-gro

127 s bind target mRNAs in high molecular weight polysome complexes, while inhibited miRNAs are stericall

128 capture hsp70 chaperones associated with the polysome complexes.

129 as a shift of TDP-43 and FUS mRNAs away from polysomes, consistent with translational silencing.

130 Polysomes contain ~1 ribosome every 200 to 900 nucleotid

131 The actively translating ribosomes (polysomes) contain amounts of proteins consistent with t

132 show that active mRNA translation complexes (polysomes) contain ribosomal protein subsets that underg

133 location of longer 3' UTR mRNAs from RNPs to polysomes correlated with the production of new miRNAs t

134 Association of mRNA with the polysomes correlates with active translation.

135 a reduction in 40S subunits and translating polysomes, correlating with reduced overall cellular pro

136 miRNA profiling of monosomes and polysomes demonstrated that miRNAs cosediment with ribos

137 ng and 40S subunit biogenesis and influences polysome density.

138 hinery concomitantly inhibits stress-induced polysome disassembly and SG assembly.

139 K85R) mutant do not prevent arsenite-induced polysome disassembly, but fails to support the SG assemb

140 ation of SG proteins to VFs was dependent on polysome dissociation and occurred via association of th

141 y performing mRNA half-life measurements and polysome distribution analysis, we found that AUF1 assoc

142 es, including involucrin, are bound to heavy polysomes during differentiation, despite decreased gene

143 3'-UTRs were preferentially associated with polysomes during oxidative stress.

144 dient purified 80S monosomes and translating polysomes each contained TbRACK1.

145 n and identified conditions that distinguish polysomes engaged in the translation of distinct cohorts

146 ly, the Sec61 complex was highly enriched in polysomes engaged in the translation of endomembrane org

147 as a direct HIF-1alpha target gene and that polysome enrichment of IGFBP3 mRNA may permit continuous

148 Hfq from polysomes extracted from wild-type cells binds RyhB in v

149 for translocation of viral RNA genome to the polysomes for efficient translation and replication.

150 upt protein synthesis and release mRNAs from polysomes for incorporation into P-bodies for degradatio

151 d to accommodate a maximum of membrane-bound polysomes for secretory protein synthesis.

152 miRNA binding sites and are thus targeted to polysomes for translation followed by degradation.

153 transport and disrupting their loading onto polysomes for translation.

154 l of the large subunit (up to 17%), impaired polysome formation (appearance of half-mers), reduced tr

155 that 2A(Pro) activity was required for viral polysome formation and stability.

156 0S ribosomal subunits that were blocked from polysome formation.

157 ing with labeled ribosomes to determine when polysomes formed.

158 et mRNA stability, but decreased the size of polysomes forming on target transcripts and lowered the

159 of the actin cytoskeleton moved Wt1 off the polysome fraction in the cytoplasm, cancelled its nucleo

160 Importantly, their localisation to the polysome fraction is independent of the RNA, suggesting

161 nd that decapping factors co-sediment in the polysome fraction of a sucrose gradient and do not alter

162 Polysome fractionation experiments indicate that dFMRP O

163 as indicated by luciferase reporter assays, polysome fractionation studies, and Western blot analysi

164 L-seq (TATL-seq), which combines TL-seq with polysome fractionation, enabled annotation of TLs, and s

165 Polysome fractionations coupled with quantitative PCR ex

166 creased viral messenger RNA association with polysome fractions and enhanced tolerance to begomovirus

167 RpL22-like detection in 80S/polysome fractions from males establishes a role for thi

168 omic analyses of HeLa cytosolic and ER-bound polysome fractions identified translocon components as s

169 e actively translating mRNAs associated with polysomes from free mRNAs.

170 Polysome gradient analyses of mutant strains revealed un

171 ation, the distribution of chimeric mRNAs on polysome gradients and the steady-state levels of expres

172 tates, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq).

173 mRNAs can be packaged in reversibly stalled polysome granules before their transport to distant syna

174 the cytoplasm, LARP6 does not associate with polysomes; however, overexpression of LARP6 blocks ribos

175 zation of RP transcripts led to retention of polysomes in a hog1Delta mutant, whereas stabilization o

176 te only in skeletal muscle, without reducing polysomes in any tissue.

177 and dynamic recruitment of messenger RNAs to polysomes in both dormant and nondormant seeds.

178 Furthermore, Htt co-sedimentation with polysomes in cytoplasmic mouse brain extracts is depende

179 nt local translation and active transport of polysomes in dendrites of primary neurons.

180 s, capture distinct mobilities of individual polysomes in different subcellular compartments, and det

181 munopurification of transcripts engaged with polysomes in pollen tubes within self-fertilized florets

182 A decay, is critical for assembly of stalled polysomes in rat hippocampal neurons derived from embryo

183 upregulated at the level of association with polysomes in response to rhizobia.

184 O, there was a reduction in the formation of polysomes in the HFD mice relative to the LFD mice, sugg

185 -'UTR-shortened transcripts efficiently form polysomes in the mTOR-activated cells, leading to increa

186 The apparent formation of polysomes in wheat germ extracts suggests that subsequen

187 RNA sequencing of polysomes indicated substantial overlap in sets of genes

188 nosines ((Cap)2G or (Cap)3G) are enriched on polysomes, indicating that RNAs synthesized from differe

189 dentified translocon components as selective polysome-interacting proteins.

190 ies with polysomes, and there is a subset of polysomes involved in myosin-dependent translation of co

191 elta cells, but their rapid association with polysomes is delayed.

192 progenitors, as well as its association with polysomes is modulated by Akt activity.

193 strated that the transport of HCV RNA on the polysomes is Stau1-dependent, being mainly localized in

194 The association of proteasomes with polysomes is weakened in srp1-49.

195 ly detected in multiple tissues, and also in polysome isolated from leaf, confirming active transcrip

196 these RNA isoforms were underrepresented in polysomes isolated from control and hypoxic plants.

197 In Drosophila, reduced IIS decreased polysome levels in fat body and gut, but reduced the rat

198 the association of eIF2 subunits, diminished polysome levels, and increased GCN4 expression indicatin

199 HuR-dependent proteins, the association with polysomes likewise depends on the eukaryotic initiation

200 directly binds Mre11 transcripts to mediate polysome loading and translation.

201 Modeling revealed that a decrease in polysome loading at night is required to balance protein

202 ases, rising metabolite levels and increased polysome loading drove an increase in fluxes.

203 tantly, the fold induction of TNF-alpha mRNA polysome loading in response to LPS stimulation is reduc

204 Leaf elongation and polysome loading in the growth zones also remain high at

205 demonstrate the essential role of selective polysome loading in this biological process.

206 inst even sudden changes in temperature, and polysome loading increases when the night temperature is

207 tion-polymerase chain reaction revealed that polysome loading remained high for much of the night in

208 In Col-0, polysome loading was 60% to 70% in the light, 40% to 45%

209 Polysome loading was investigated during the diurnal cyc

210 nses of the metabolome and transcriptome and polysome loading, as a qualitative proxy for protein syn

211 t correlated with overall and with cytosolic polysome loading.

212 h is mechanistically traced to a decrease in polysome loading.

213 med target mRNA localization to the ER-bound polysomes manifested as the earliest event, which is fol

214 We propose that polysomes may act as antennae that enhance the rates of

215 Here we used a systems approach by combining polysome mRNA profiling and bioinformatics to identify R

216 uppressed receptor translation by inhibiting polysome-mRNA association.

217 ell line to fit thousands of images of human polysomes obtained by atomic force microscopy, from whic

218 Both signaling pathways enhanced polysome occupancy and eukaryotic initiation factor 4E (

219 The effect of Jak-Stat-Pim signaling on polysome occupancy and expression of GW182 protein was g

220 to maximize conformational entropy, and the polysomes occupy the empty space near the walls to maxim

221 cells binds RyhB in vitro, whereas Hfq from polysomes of a relA mutant strain shows no binding.

222 ail lengths were similar for target mRNAs on polysomes or in non-translating mRNPs, and the presence

223 Cellular mRNAs can be actively translated in polysomes or physically sequestered in cytoplasmic proce

224 of Snail1 mRNA into the actively translated polysome pool accompanied by accumulation of the EMT tra

225 otifs in mRNAs that either enter or exit the polysome pool during mouse oocyte maturation.

226 In addition, polysome profile analyses indicate that, on stress, ribo

227 Polysome profile analyses reveal the accumulation of hal

228 Finally, a polysome profile analysis using sucrose gradient ultrace

229 We fractionated a polysome profile and reconstructed transcript isoforms f

230 nction using 10 point mutations altering the polysome profile.

231 , we performed a genome-wide analysis of the polysome-profiled mRNAs by using an Affymetrix GeneChip

232 Polysome profiles confirmed the decreased translational

233 ein synthesis with one important difference: Polysome profiles observed immediately after eIF5A deple

234 Analyses of polysome profiles revealed that key differentiation gene

235 in proplatelet-producing MKs in 2D DIGE and polysome profiling analyses, respectively.

236 analysis of CD8 T-cell activation, combining polysome profiling and microarray analysis.

237 ss of PUF3 on gene expression using mRNA and polysome profiling and quantitative proteomics.

238 -)), and two independent translation assays, polysome profiling and radiolabeled amino acid incorpora

239 Furthermore, using polysome profiling and ribosome profiling, we failed to

240 Combining polysome profiling and RNA sequencing, we identified the

241 We used polysome profiling by RNA-Seq to quantify translational

242 Polysome profiling of the rpl4d mutant showed reduction

243 Polysome profiling revealed that translation of GluA1 an

244 Here, we show by polysome profiling that inhibin betaA is translationally

245 blastoma to ionizing radiation (IR), we used polysome profiling to define the IR-induced translatomes

246 2D difference gel electrophoresis (DIGE) and polysome profiling, respectively.

247 As measured by polysome profiling, the translational efficiencies of in

248 ight stress in Arabidopsis thaliana by using polysomes profiling.

249 l oligopyrimidine motif) and TOP-like mRNAs, polysome-profiling indicated that MTOR also modulates tr

250 this regulation, we developed a genome-wide polysome-profiling strategy using stage-matched WT and e

251 In untreated conditions, Puf3p migrates with polysomes rather than ribosome-free fractions, but this

252 dependent 5'TOP mRNA translation repression, polysome release, and accumulation in stress granules.

253 R1 activation enhanced recruitment of RNA to polysomes, resulting in a marked increase in protein exp

254 , it facilitates association of MDR1 mRNA to polysomes, resulting in increased translation, and AEG-1

255 tion of factor XII (FXII) messenger RNA with polysomes, resulting in increased translation.

256 isoform mRNAs lost from the eif3ha morphant polysomes, revealing a mechanism by which lens developme

257 action, which we term Transcript Isoforms in Polysomes sequencing (TrIP-seq).

258 Overall polysome sequestration contributes to an increase of cel

259 Here we report a novel method--miRNA Polysome Shift Assay (miPSA)--for direct measurement of

260 ng RatA showed that 70S ribosomes as well as polysomes significantly decreased with concomitant incre

261 ffects of eIF5A depletion are a reduction in polysome size concomitant with eIF5A depletion.

262 -intact tissue culture cells, we performed a polysome solubilization screen and identified conditions

263 The potential role of the polysome structure during protein translation and the re

264 lored translational controls embedded in the polysome structure.

265 Electron microscopic analysis of polysome structures in translation lysates revealed a ti

266 mRNA stability and polysome studies suggest IGF2BP1 mediates posttranscript

267 NS5A associated with eIF4F complex and polysomes, suggesting its active involvement in host tra

268 th retained U12-type introns can be found in polysomes, suggesting that splicing efficiency can alter

269 F-1alpha mRNA transport and releases it from polysomes, suppressing its translation.

270 specific patterns of mRNA recruitment to the polysomes that are synchronized with the cell cycle.

271 decreasing the proportion of mef2ca mRNA in polysomes, the levels of Mef2c and slow myosin heavy cha

272 nge in the levels of Ribosomal protein L7 in polysomes, thereby regulating neocortical translation ma

273 represses the association of MOR1 mRNA with polysomes through the MOR1 3'-UTR.

274 esponded with a shift in cyclin B1 mRNA from polysome to nonpolysome fractions.

275 Gemin5 redistributes the SMN mRNA from heavy polysomes to lighter polysomes and monosomes, suggesting

276 further examined their potency at converting polysomes to monosomes across other commonly used model

277 osomal integrity while thoroughly converting polysomes to monosomes in all examined species.

278 type cells and the shift in translation from polysomes to monosomes is attenuated, suggesting puf3Del

279 bosomal particles and complete conversion of polysomes to monosomes.

280 g TNFalpha mRNA from polyribosome-associated polysomes to monosomes.

281 forms of recapping targets redistribute from polysomes to non-translating mRNPs, and recapping is all

282 he redistribution of target transcripts from polysomes to non-translating mRNPs, where they accumulat

283 with a shift of ubiquitin mRNAs from larger polysomes to non-translating ribosomes.

284 futsch mRNA shift from actively translating polysomes to nontranslating ribonuclear protein particle

285 (Dc,60S = 0.311 microm(2)/s), whereas entire polysomes underwent long-range motility along microtubul

286 rmine the partitioning of ribosomes into 70S-polysomes versus free 30S and 50S subunits.

287 percentage of the HAC1 mRNA associating with polysomes versus free ribosomal subunits.

288 ned that CSP1 associates with polyribosomes (polysomes) via an RNA-mediated interaction.

289 ation, the association of mRNAs and sRNAs to polysomes was characterized in roots of the model legume

290 immediate-early, early, and late mRNAs with polysomes was not affected by eIF4F disruption.

291 wnregulated and exclusively localized in the polysomes when Stau1 is overexpressed.

292 -independent manner, were shifted to heavier polysomes whereas mRNAs encoding GAPDH, actin, L32, and

293 ment of early endosomes randomly distributes polysomes, which may ensure the even distribution of the

294 ated through decreased mRNA association with polysomes, which requires microRNA23b (miRNA23b), a spec

295 s C), but did not influence global levels of polysomes, which were minimally perturbed by above freez

296 on results in decreased RHA association with polysomes while increasing Lin28 expression leads to ele

297 Cbc1 associates with polysomes, while the deletion of the CBC1 gene causes hy

298 Immunoprecipitation of polysomes with a monoclonal antibody against HA yields r

299 Loss of polysomes with increased 80S monosomes in the polyamine-

300 F correlated with alterations in the size of polysomes with ribosomes present every 250 to 500 nucleo