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1 esis as the nascent proteins emerge from the polysome.
2 ctors may be constitutively localised to the polysome.
3 y and the association of the transcript with polysomes.
4 esent in all subpopulations of ER-associated polysomes.
5 se in the amount of grk mRNA associated with polysomes.
6 uces association of the UME6 transcript with polysomes.
7 s with the return of the stabilized mRNAs to polysomes.
8 3 translation and TP53 mRNA association with polysomes.
9 mRNAs into the active translation sites, the polysomes.
10 a2(I) mRNA is unrestrictedly loaded onto the polysomes.
11 p1 mutant for membrane-bound, but not total, polysomes.
12 ncreasing the loading of CBP/p300 mRNAs onto polysomes.
13 es with 40S ribosomal subunits and also with polysomes.
14  and it associates with mRNAs assembled into polysomes.
15 existing DHX33 mRNAs to actively translating polysomes.
16  of both PHO1;2 and cis-NATPHO1;2 toward the polysomes.
17 and the loading of CBP/p300 transcripts onto polysomes.
18 nally, IGFBP3 mRNA was found enriched to the polysomes.
19 onosomes and increased their target mRNAs in polysomes.
20 , of mRNAs encoding particular proteins from polysomes.
21 he cell coincident with their depletion from polysomes.
22 the tool to isolate coupled mitochondria and polysomes.
23 ap-dependent manner, were shifted to lighter polysomes.
24 te translation by actively recruiting RHA to polysomes.
25 lasm between the cytosol and the translating polysomes.
26 is present on many proteins that form active polysomes.
27 rotein synthesis rate and formation of large polysomes.
28 all of which are localized by membrane-bound polysomes.
29 ction motifs decrease LARP4 association with polysomes.
30  associated with cytosolic 80S ribosomes and polysomes.
31  transcript was present in RNA isolated from polysomes.
32 , and there was marked depletion of HuR from polysomes.
33  are detected in both ribonucleoproteins and polysomes.
34 PCBP 1 and 2, also cofractionated with viral polysomes.
35 ns uL4 or uL22 are still capable of entering polysomes.
36 cay (NMD), even though they were detected in polysomes.
37 ycolysis decreases TNF mRNA association with polysomes.
38 et of oxidative stress-responsive mRNAs into polysomes.
39  mammalian cells are in turn associated with polysomes.
40 vative, the chromatoid body, as well as with polysomes [9].
41 in vitro, ribosome transit rates slowed, and polysomes accumulated in intact cells, suggesting defect
42 oping multicolor probes, we showed that most polysomes act independently; however, a small fraction (
43 subunits in sucrose gradients and repopulate polysomes after a short starvation-induced translational
44           ADH2 mRNA rapidly dissociated from polysomes after glucose repletion, and sequences bound b
45    This heightened association of miRNA with polysomes also elicits reduced degradation of target mRN
46                                              Polysome analyses and in vitro reconstitution of initiat
47 tive reverse transcriptase PCR (qRT-PCR) and polysome analyses were performed.
48                                              Polysome analysis indicated that EF-P functions in trans
49                          A combined eRIP and polysome analysis of early stage differentiation of hESC
50 lational profiling by microarray analysis of polysome and monosome associated mRNAs in wild-type and
51 ighly hypoxia-induced mRNAs are recruited to polysomes and actively translated, whereas other cellula
52 . cerevisiae resulted in the accumulation of polysomes and an increase in ribosomal transit times.
53                      Cul4B is present on the polysomes and colocalizes with TTP to exosomes and proce
54 e dynamics of messenger RNA association with polysomes and compared the transcriptome with the transl
55 l Ccr4-Not complex in yeast, associates with polysomes and contributes to the negative regulation of
56 levels of actb mRNA associated with synaptic polysomes and diminished levels of synaptic actb protein
57 liced c-myb transcripts were associated with polysomes and encoded a series of c-Myb proteins with id
58 the SMN mRNA from heavy polysomes to lighter polysomes and monosomes, suggesting that Gemin5 function
59 ssembled ribosomes exist in two populations: polysomes and monosomes.
60  correlated with a global reduction in heavy polysomes and overall repression of translation.
61                        Depletion of NAC from polysomes and re-localisation to protein aggregates is o
62 icient in loading uORF-containing mRNAs onto polysomes and stimulates translation in protoplasts, and
63 rent supra-ribosomal building blocks forming polysomes and suggest the presence of unexplored transla
64                    In normal conditions, 70S-polysomes and the chromosomal DNA segregate, while 30S a
65 tively translating mRNAs are associated with polysomes and the newly synthesized peptide chains are c
66 ion influenced recruitment of mRNAs to heavy polysomes and translation of subsets of genes.
67 in annotated 5'-UTRs were overrepresented in polysomes and were as stable as canonical mRNA isoforms.
68 s are subject to differential recruitment to polysomes, and expose the importance of selective mRNA t
69                    Rather, they are found on polysomes, and genetic analysis indicates that translati
70 ssion, promotes cap-dependent translation in polysomes, and reduces the anti-proliferative effect of
71 messenger ribonucleoprotein for loading onto polysomes, and reduction of Cul4B expression shunts the
72             Nonmuscle myosin copurifies with polysomes, and there is a subset of polysomes involved i
73 rmore, phosphorylated eIF4E relocates to the polysomes, and this contributes to changes in the transl
74 m a stable complex with the 70S ribosome and polysomes, and we demonstrate the proximity in vivo of r
75 iation of the mRNA with actively translating polysomes; and de novo MMP-9 protein synthesis were obta
76 n kinesin-3 and dynein-driven EEs, where the polysomes appeared to translate EE-associated mRNA into
77 ree 40S subunits are increased, and half-mer polysomes are accumulated.
78                 We also observe that DYNC1H1 polysomes are actively transported by motors, which may
79                            Here we show that polysomes are primarily formed by three distinct classes
80 ort and the distribution of the UL84 mRNA on polysomes are unaffected.
81 multaneously translate the same mRNA strand (polysomes) are represented as freely jointed chains of h
82 on microscopy to image ex-vivo-derived human polysomes as a source of actively translating ribosomes.
83       Finally, some DDX6 was associated with polysomes, as previously reported in yeast.
84 ll organization of mRNA and ribosomes within polysomes, as well as the possible role of this organiza
85                                  Chloroplast polysome assembly is reduced in csp41b-1/csp41a-1* mutan
86 crease in mRNA levels as well as a defect in polysome assembly that was independent of mRNA abundance
87 n about how proteins emerging from different polysomes associate to form hetero-oligomeric channels.
88  maydis, Higuchi et al. now demonstrate that polysomes associate with early endosomes that undergo ki
89      Collectively, our results indicate that polysomes associate with moving EEs and that "off- and r
90 at the mRNA encoding plexin A remains highly polysome associated during stress and escapes degradatio
91 ticles lacking srRNA 4 and reduced levels of polysome associated ribosomes.
92 NA targeted mRNAs are invariably found to be polysome associated; observations that appear to be at f
93           ARF caused a dramatic reduction in polysome-associated DHX33 mRNAs, while Ras(V12) led to a
94    Although psbA and rbcL mRNAs are normally polysome-associated in the mutant, petD-containing RNAs
95        Genome-wide analysis of cytosolic and polysome-associated messenger RNA (mRNA) revealed not on
96 tment did not change overall MOR transcript, polysome-associated mRNA declined in a let-7-dependent m
97      In contrast, the protein expression and polysome-associated mRNA levels of both genes were incre
98  combinatorial biotin transfer system, where polysome-associated mRNAs are selectively recovered from
99 logy clustering showed that the functions of polysome-associated transcripts differed between dormant
100                               The changes in polysome-associated tRNA levels reflect the codon usage
101 anged following viral infection, whereas the polysome-associated tRNA population changes dramatically
102  delineated how a target mRNA passes through polysome association and Ago2 interaction steps on rough
103                                              Polysome association and detection of a low abundant 13.
104 and eIF4E2 are elucidated by observing their polysome association and the status of mammalian target
105  This novel combination results in increased polysome association of mda-7/IL-24 mRNA, activation of
106                         Large differences in polysome association of miRNA target RNAs are found betw
107                        We next observed that polysome association of nad7 mature mRNA is specifically
108 as promoted by UPF1 depletion, which induced polysome association of PTC-containing mRNAs, increased
109 riptional activity, and had no effect on the polysome association profile of HIF-1alpha.
110                                      Reduced polysome association was observed for additional key reg
111 exes from target mRNAs, leading to increased polysome association, translation, and stability of miRN
112 und mRNAs, we found that La binds 34% of the polysome bound mRNAs and regulates the expression of a s
113                                   Changes in polysome-bound mRNA (translatome) are correlated closely
114 ling of the rpl4d mutant showed reduction in polysome-bound mRNA compared with wild-type, but no sign
115            RNA-sequencing after isolation of polysome-bound mRNAs allows for cell-type-specific, geno
116 a in the cytoplasm by microarray analysis of polysome-bound mRNAs, we found that La binds 34% of the
117 is influenced by ionizing radiation, we used polysome-bound RNA to generate gene translation profiles
118 impact on the association of HCMV mRNAs with polysomes but significantly diminished the translation e
119 so determines the specificity of neocortical polysomes by defining their combinatorial composition of
120 nt of elongation, TOP mRNAs are recruited on polysomes causing a relative increase in the synthesis o
121 pholipase1 (Lypla1)--selectively entered the polysome compartment.
122 on and are a result of its direct binding to polysomes, complex formation with cellular RNAs (which i
123 lyzing mRNAs that dynamically associate with polysome complexes as neocortical development progresses
124 a-up-regulated gene transcripts increased in polysome complexes during the stress, but the number of
125 f of the cellular mRNAs were restricted from polysome complexes during the stress, with little or no
126         Ribosomal protein specificity within polysome complexes is regulated by the arrival of in-gro
127 s bind target mRNAs in high molecular weight polysome complexes, while inhibited miRNAs are stericall
128 capture hsp70 chaperones associated with the polysome complexes.
129 as a shift of TDP-43 and FUS mRNAs away from polysomes, consistent with translational silencing.
130                                              Polysomes contain ~1 ribosome every 200 to 900 nucleotid
131          The actively translating ribosomes (polysomes) contain amounts of proteins consistent with t
132 show that active mRNA translation complexes (polysomes) contain ribosomal protein subsets that underg
133 location of longer 3' UTR mRNAs from RNPs to polysomes correlated with the production of new miRNAs t
134                 Association of mRNA with the polysomes correlates with active translation.
135  a reduction in 40S subunits and translating polysomes, correlating with reduced overall cellular pro
136             miRNA profiling of monosomes and polysomes demonstrated that miRNAs cosediment with ribos
137 ng and 40S subunit biogenesis and influences polysome density.
138 hinery concomitantly inhibits stress-induced polysome disassembly and SG assembly.
139 K85R) mutant do not prevent arsenite-induced polysome disassembly, but fails to support the SG assemb
140 ation of SG proteins to VFs was dependent on polysome dissociation and occurred via association of th
141 y performing mRNA half-life measurements and polysome distribution analysis, we found that AUF1 assoc
142 es, including involucrin, are bound to heavy polysomes during differentiation, despite decreased gene
143  3'-UTRs were preferentially associated with polysomes during oxidative stress.
144 dient purified 80S monosomes and translating polysomes each contained TbRACK1.
145 n and identified conditions that distinguish polysomes engaged in the translation of distinct cohorts
146 ly, the Sec61 complex was highly enriched in polysomes engaged in the translation of endomembrane org
147  as a direct HIF-1alpha target gene and that polysome enrichment of IGFBP3 mRNA may permit continuous
148                                     Hfq from polysomes extracted from wild-type cells binds RyhB in v
149 for translocation of viral RNA genome to the polysomes for efficient translation and replication.
150 upt protein synthesis and release mRNAs from polysomes for incorporation into P-bodies for degradatio
151 d to accommodate a maximum of membrane-bound polysomes for secretory protein synthesis.
152 miRNA binding sites and are thus targeted to polysomes for translation followed by degradation.
153  transport and disrupting their loading onto polysomes for translation.
154 l of the large subunit (up to 17%), impaired polysome formation (appearance of half-mers), reduced tr
155 that 2A(Pro) activity was required for viral polysome formation and stability.
156 0S ribosomal subunits that were blocked from polysome formation.
157 ing with labeled ribosomes to determine when polysomes formed.
158 et mRNA stability, but decreased the size of polysomes forming on target transcripts and lowered the
159  of the actin cytoskeleton moved Wt1 off the polysome fraction in the cytoplasm, cancelled its nucleo
160       Importantly, their localisation to the polysome fraction is independent of the RNA, suggesting
161 nd that decapping factors co-sediment in the polysome fraction of a sucrose gradient and do not alter
162                                              Polysome fractionation experiments indicate that dFMRP O
163  as indicated by luciferase reporter assays, polysome fractionation studies, and Western blot analysi
164 L-seq (TATL-seq), which combines TL-seq with polysome fractionation, enabled annotation of TLs, and s
165                                              Polysome fractionations coupled with quantitative PCR ex
166 creased viral messenger RNA association with polysome fractions and enhanced tolerance to begomovirus
167                  RpL22-like detection in 80S/polysome fractions from males establishes a role for thi
168 omic analyses of HeLa cytosolic and ER-bound polysome fractions identified translocon components as s
169 e actively translating mRNAs associated with polysomes from free mRNAs.
170                                              Polysome gradient analyses of mutant strains revealed un
171 ation, the distribution of chimeric mRNAs on polysome gradients and the steady-state levels of expres
172 tates, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq).
173  mRNAs can be packaged in reversibly stalled polysome granules before their transport to distant syna
174 the cytoplasm, LARP6 does not associate with polysomes; however, overexpression of LARP6 blocks ribos
175 zation of RP transcripts led to retention of polysomes in a hog1Delta mutant, whereas stabilization o
176 te only in skeletal muscle, without reducing polysomes in any tissue.
177 and dynamic recruitment of messenger RNAs to polysomes in both dormant and nondormant seeds.
178       Furthermore, Htt co-sedimentation with polysomes in cytoplasmic mouse brain extracts is depende
179 nt local translation and active transport of polysomes in dendrites of primary neurons.
180 s, capture distinct mobilities of individual polysomes in different subcellular compartments, and det
181 munopurification of transcripts engaged with polysomes in pollen tubes within self-fertilized florets
182 A decay, is critical for assembly of stalled polysomes in rat hippocampal neurons derived from embryo
183 upregulated at the level of association with polysomes in response to rhizobia.
184 O, there was a reduction in the formation of polysomes in the HFD mice relative to the LFD mice, sugg
185 -'UTR-shortened transcripts efficiently form polysomes in the mTOR-activated cells, leading to increa
186                    The apparent formation of polysomes in wheat germ extracts suggests that subsequen
187                            RNA sequencing of polysomes indicated substantial overlap in sets of genes
188 nosines ((Cap)2G or (Cap)3G) are enriched on polysomes, indicating that RNAs synthesized from differe
189 dentified translocon components as selective polysome-interacting proteins.
190 ies with polysomes, and there is a subset of polysomes involved in myosin-dependent translation of co
191 elta cells, but their rapid association with polysomes is delayed.
192 progenitors, as well as its association with polysomes is modulated by Akt activity.
193 strated that the transport of HCV RNA on the polysomes is Stau1-dependent, being mainly localized in
194          The association of proteasomes with polysomes is weakened in srp1-49.
195 ly detected in multiple tissues, and also in polysome isolated from leaf, confirming active transcrip
196  these RNA isoforms were underrepresented in polysomes isolated from control and hypoxic plants.
197         In Drosophila, reduced IIS decreased polysome levels in fat body and gut, but reduced the rat
198 the association of eIF2 subunits, diminished polysome levels, and increased GCN4 expression indicatin
199 HuR-dependent proteins, the association with polysomes likewise depends on the eukaryotic initiation
200  directly binds Mre11 transcripts to mediate polysome loading and translation.
201         Modeling revealed that a decrease in polysome loading at night is required to balance protein
202 ases, rising metabolite levels and increased polysome loading drove an increase in fluxes.
203 tantly, the fold induction of TNF-alpha mRNA polysome loading in response to LPS stimulation is reduc
204                          Leaf elongation and polysome loading in the growth zones also remain high at
205  demonstrate the essential role of selective polysome loading in this biological process.
206 inst even sudden changes in temperature, and polysome loading increases when the night temperature is
207 tion-polymerase chain reaction revealed that polysome loading remained high for much of the night in
208                                    In Col-0, polysome loading was 60% to 70% in the light, 40% to 45%
209                                              Polysome loading was investigated during the diurnal cyc
210 nses of the metabolome and transcriptome and polysome loading, as a qualitative proxy for protein syn
211 t correlated with overall and with cytosolic polysome loading.
212 h is mechanistically traced to a decrease in polysome loading.
213 med target mRNA localization to the ER-bound polysomes manifested as the earliest event, which is fol
214                              We propose that polysomes may act as antennae that enhance the rates of
215 Here we used a systems approach by combining polysome mRNA profiling and bioinformatics to identify R
216 uppressed receptor translation by inhibiting polysome-mRNA association.
217 ell line to fit thousands of images of human polysomes obtained by atomic force microscopy, from whic
218             Both signaling pathways enhanced polysome occupancy and eukaryotic initiation factor 4E (
219      The effect of Jak-Stat-Pim signaling on polysome occupancy and expression of GW182 protein was g
220  to maximize conformational entropy, and the polysomes occupy the empty space near the walls to maxim
221  cells binds RyhB in vitro, whereas Hfq from polysomes of a relA mutant strain shows no binding.
222 ail lengths were similar for target mRNAs on polysomes or in non-translating mRNPs, and the presence
223 Cellular mRNAs can be actively translated in polysomes or physically sequestered in cytoplasmic proce
224  of Snail1 mRNA into the actively translated polysome pool accompanied by accumulation of the EMT tra
225 otifs in mRNAs that either enter or exit the polysome pool during mouse oocyte maturation.
226                                 In addition, polysome profile analyses indicate that, on stress, ribo
227                                              Polysome profile analyses reveal the accumulation of hal
228                                   Finally, a polysome profile analysis using sucrose gradient ultrace
229                            We fractionated a polysome profile and reconstructed transcript isoforms f
230 nction using 10 point mutations altering the polysome profile.
231 , we performed a genome-wide analysis of the polysome-profiled mRNAs by using an Affymetrix GeneChip
232                                              Polysome profiles confirmed the decreased translational
233 ein synthesis with one important difference: Polysome profiles observed immediately after eIF5A deple
234                                  Analyses of polysome profiles revealed that key differentiation gene
235  in proplatelet-producing MKs in 2D DIGE and polysome profiling analyses, respectively.
236 analysis of CD8 T-cell activation, combining polysome profiling and microarray analysis.
237 ss of PUF3 on gene expression using mRNA and polysome profiling and quantitative proteomics.
238 -)), and two independent translation assays, polysome profiling and radiolabeled amino acid incorpora
239                           Furthermore, using polysome profiling and ribosome profiling, we failed to
240                                    Combining polysome profiling and RNA sequencing, we identified the
241                                      We used polysome profiling by RNA-Seq to quantify translational
242                                              Polysome profiling of the rpl4d mutant showed reduction
243                                              Polysome profiling revealed that translation of GluA1 an
244                             Here, we show by polysome profiling that inhibin betaA is translationally
245 blastoma to ionizing radiation (IR), we used polysome profiling to define the IR-induced translatomes
246 2D difference gel electrophoresis (DIGE) and polysome profiling, respectively.
247                               As measured by polysome profiling, the translational efficiencies of in
248 ight stress in Arabidopsis thaliana by using polysomes profiling.
249 l oligopyrimidine motif) and TOP-like mRNAs, polysome-profiling indicated that MTOR also modulates tr
250  this regulation, we developed a genome-wide polysome-profiling strategy using stage-matched WT and e
251 In untreated conditions, Puf3p migrates with polysomes rather than ribosome-free fractions, but this
252 dependent 5'TOP mRNA translation repression, polysome release, and accumulation in stress granules.
253 R1 activation enhanced recruitment of RNA to polysomes, resulting in a marked increase in protein exp
254 , it facilitates association of MDR1 mRNA to polysomes, resulting in increased translation, and AEG-1
255 tion of factor XII (FXII) messenger RNA with polysomes, resulting in increased translation.
256  isoform mRNAs lost from the eif3ha morphant polysomes, revealing a mechanism by which lens developme
257 action, which we term Transcript Isoforms in Polysomes sequencing (TrIP-seq).
258                                      Overall polysome sequestration contributes to an increase of cel
259         Here we report a novel method--miRNA Polysome Shift Assay (miPSA)--for direct measurement of
260 ng RatA showed that 70S ribosomes as well as polysomes significantly decreased with concomitant incre
261 ffects of eIF5A depletion are a reduction in polysome size concomitant with eIF5A depletion.
262 -intact tissue culture cells, we performed a polysome solubilization screen and identified conditions
263                    The potential role of the polysome structure during protein translation and the re
264 lored translational controls embedded in the polysome structure.
265             Electron microscopic analysis of polysome structures in translation lysates revealed a ti
266                           mRNA stability and polysome studies suggest IGF2BP1 mediates posttranscript
267       NS5A associated with eIF4F complex and polysomes, suggesting its active involvement in host tra
268 th retained U12-type introns can be found in polysomes, suggesting that splicing efficiency can alter
269 F-1alpha mRNA transport and releases it from polysomes, suppressing its translation.
270 specific patterns of mRNA recruitment to the polysomes that are synchronized with the cell cycle.
271  decreasing the proportion of mef2ca mRNA in polysomes, the levels of Mef2c and slow myosin heavy cha
272 nge in the levels of Ribosomal protein L7 in polysomes, thereby regulating neocortical translation ma
273  represses the association of MOR1 mRNA with polysomes through the MOR1 3'-UTR.
274 esponded with a shift in cyclin B1 mRNA from polysome to nonpolysome fractions.
275 Gemin5 redistributes the SMN mRNA from heavy polysomes to lighter polysomes and monosomes, suggesting
276 further examined their potency at converting polysomes to monosomes across other commonly used model
277 osomal integrity while thoroughly converting polysomes to monosomes in all examined species.
278 type cells and the shift in translation from polysomes to monosomes is attenuated, suggesting puf3Del
279 bosomal particles and complete conversion of polysomes to monosomes.
280 g TNFalpha mRNA from polyribosome-associated polysomes to monosomes.
281 forms of recapping targets redistribute from polysomes to non-translating mRNPs, and recapping is all
282 he redistribution of target transcripts from polysomes to non-translating mRNPs, where they accumulat
283  with a shift of ubiquitin mRNAs from larger polysomes to non-translating ribosomes.
284  futsch mRNA shift from actively translating polysomes to nontranslating ribonuclear protein particle
285 (Dc,60S = 0.311 microm(2)/s), whereas entire polysomes underwent long-range motility along microtubul
286 rmine the partitioning of ribosomes into 70S-polysomes versus free 30S and 50S subunits.
287 percentage of the HAC1 mRNA associating with polysomes versus free ribosomal subunits.
288 ned that CSP1 associates with polyribosomes (polysomes) via an RNA-mediated interaction.
289 ation, the association of mRNAs and sRNAs to polysomes was characterized in roots of the model legume
290  immediate-early, early, and late mRNAs with polysomes was not affected by eIF4F disruption.
291 wnregulated and exclusively localized in the polysomes when Stau1 is overexpressed.
292 -independent manner, were shifted to heavier polysomes whereas mRNAs encoding GAPDH, actin, L32, and
293 ment of early endosomes randomly distributes polysomes, which may ensure the even distribution of the
294 ated through decreased mRNA association with polysomes, which requires microRNA23b (miRNA23b), a spec
295 s C), but did not influence global levels of polysomes, which were minimally perturbed by above freez
296 on results in decreased RHA association with polysomes while increasing Lin28 expression leads to ele
297                         Cbc1 associates with polysomes, while the deletion of the CBC1 gene causes hy
298                       Immunoprecipitation of polysomes with a monoclonal antibody against HA yields r
299                                      Loss of polysomes with increased 80S monosomes in the polyamine-
300 F correlated with alterations in the size of polysomes with ribosomes present every 250 to 500 nucleo

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