ライフサイエンスコーパス: position effect

1 in position-dependent transgene expression ("position effects").

2 result in PLP1 gene silencing by virtue of a position effect.

3 ears to be a previously unidentified form of position effect.

4 s in MATa cells, indicating a potential tRNA position effect.

5 es are not dominant suppressors of telomeric position effect.

6 1 (HP1) at telomeres and suppresses telomere position effect.

7 lomeric structure and abolishes the telomere position effect.

8 nism is unknown but is postulated to involve position effect.

9 ally in linkage equilibrium, and there is no position effect.

10 mmediate region that might be inactivated by position effect.

11 sed rDNA function is caused by a chromosomal position effect.

12 es transcription of adjacent genes through a position effect.

13 rodeletions upstream of FOXF1, implicating a position effect.

14 least one paralog was regulated by telomere position effect.

15 int, providing a potential mechanism for the position effect.

16 to exemplify the utility of our analysis of position effect.

17 ion breakpoints disrupt a gene(s) or cause a position effect.

18 ced telomere elongation and altered telomere position effect.

19 some I ORF is silenced by a natural telomere position effect.

20 ults in insertional mutagenesis and variable position effects.

21 netic discrimination are highly sensitive to position effects.

22 n due to copy number differences and genomic position effects.

23 ator to relieve silencing owing to chromatin position effects.

24 larger LCR vector to stable and variegating position effects.

25 al of the MARs, the locus becomes subject to position effects.

26 ncer interactions and buffer transgenes from position effects.

27 pes with mice carrying transgenes subject to position effects.

28 linker histones can attenuate or accentuate position effects.

29 cus structures and influenced by chromosomal position effects.

30 rences in vector copy number and chromosomal position effects.

31 the ability to screen reporter genes against position effects.

32 lator to protect expression from chromosomal position effects.

33 by protecting these vectors from chromosomal position effects.

34 ut were differentially sensitive to numerous position effects.

35 egion could protect a retrovirus vector from position effects.

36 ctional manner, and protect a transgene from position effects.

37 control because it is markedly influenced by position effects.

38 and mature T cells but is subject to severe position effects.

39 sitize or protect the globin transgenes from position effects.

40 tion and promoter regions are categorized as position effects.

41 rial search has been the existence of serial position effects.

42 transgenic mouse embryos this was subject to position effects.

43 ers and protects transgenes from chromosomal position effects.

44 rotect a transgene from negative chromosomal position effects.

45 e expression from integration site-dependent position effects.

46 nd transgene expression can be influenced by position effects.

47 sitions and are therefore subject to varying position effects.

48 osed that DNA distortion was involved in the positioning effect.

49 olecules which appeared to be related to the positioning effect.

50 These rearrangements suggest that position effects acting from a great distance regulate S

51 tamination of the neural data because of eye position effects, all experiments with significant effec

52 In addition, position effects also contributed to extinction of many

53 Position effects also represent potentially insurmountab

54 Telomere position effect, an epigenetic phenomenon, has been prop

55 retrovirally transferred genes is variable (position effect and expression variegation) because retr

56 late reporter transgenes against chromosomal position effects and can block enhancer-promoter interac

57 scriptional interference could underlie some position effects and contribute to the regulation of gen

58 independent manner, giving rise to chromatin position effects and epigenetic memory.

59 nistic parallels to the general phenomena of position effects and gene silencing.

60 insulator are protected against chromosomal position effects and gradual extinction of expression du

61 has been variable in red blood cells due to position effects and retrovirus silencing.

62 When position effects and side chain-side chain interactions

63 a-GFP was more efficient and less subject to position effects and silencing than transduction with a

64 criptional regulatory information to predict position effects and their association with pathogenic p

65 However, low-level expression, position effects and transcriptional silencing hampered

66 variation to assess and minimize steric and positioning effects and determine effective molarities t

67 equences, repressing neighboring genes (tRNA position effect), and acting as a barrier to the spread

68 contributes to the likelihood of variegating position effects, and can affect the magnitude of its tr

69 nformation, updating QTL parameters, such as position, effects, and the allele flow relationships is

70 The positioning effects appeared to be determined by the for

71 pools composed of 50 clones, indicating that position effects are averaged in pools composed by large

72 transcriptional enhancement and variegating position effects are caused by fundamentally different b

73 However, whether stable and variegated position effects are equally overcome by an intact LCR h

74 e, we examine the human disorders where such position effects are implicated.

75 pporting the latter capacity that suppresses position effects are incompletely understood.

76 Transposon position effects are now hypothesized to balance the exp

77 based on their location in the genome; these position effects are of interest because they reflect th

78 is known to affect its expression, but such position effects are poorly understood in bacteria.

79 major determinant of gene activity; however, position effects are rarely central to current discussio

80 onsider interactions between nucleosomes and positioning effects as perturbations on a random positio

81 an autonomous unit resistant to chromosomal position effects, as evidenced by numerous transgenic mo

82 Using both a chromosomal position-effect assay and an enhancer-blocking assay, we

83 be the consequence of a more global telomere position effect at the level of chromatin structure.

84 n and experimental confirmation of tRNA gene position effects at native chromosomal loci.

85 s with different eye color phenotypes due to position effects at the sites of transgene insertion.

86 s but did undergo mild to severe variegating position effects at three of the four non-centromeric in

87 cause chromatin insulation, i.e., to relieve position effects at transgene integration sites in cultu

88 e 19qtel is probably inactive by virtue of a position effect, because a healthy male sibling carries

89 lanced rearrangements with what appear to be position effect breakpoints 3' of PAX6: (a) a t(7;11) wi

90 locus were not subject to detectable stable position effects but did undergo mild to severe variegat

91 the efficient elimination of variability of position effects by developing a PhiC31 integrase-based

92 ic constructs are protected from chromosomal position effects by flanking insulator elements, the sup

93 construct that was buffered from chromosomal position effects by flanking insulator elements.

94 y close to a tRNA gene, shows that this tRNA position effect can operate on a native chromosomal gene

95 These position effects can be cured by specific DNA elements,

96 Position effects can complicate transgene analyses.

97 Correcting these position effects can substantially improve measurement a

98 sites in the genome may eliminate unwanted 'position effects' caused by the random integration of ex

99 the effect of telomere proximity on telomere position effect, chromosome healing, and sensitivity to

100 Chromosome position effects combined with transgene silencing of mu

101 The observed CN position effect conforms to the design concept.

102 Position-effect control at the silent mat2-mat3 interval

103 Associated position effects, copy number differences and multigene

104 cking") and acts as a barrier to chromosomal position effect (CPE) when it surrounds a stably integra

105 bution of chromosomal aberrations leading to position effects (disruption of a gene's regulatory envi

106 ields and suppression efficiencies (i.e. the position effect) do not correlate with any of the report

107 d silencing of transposons near genes causes position-effect down-regulation.

108 eved, certain factors, including chromosomal position effects due to random integration of the transg

109 ne integration at the same locus, as well as position effects due to random integration.

110 the locus to become sensitive to chromosomal position effects emanating from distal sequences.

111 ize the importance of overcoming detrimental position effects for consistent therapeutic globin vecto

112 tream of SOX9, making this the longest-range position effect found in the field of human genetics and

113 , are essentially random and are subject to 'position effects' from nearby endogenous regulatory elem

114 adjacent to telomeres, also called telomere position effect, has been hypothesized as a possible mec

115 ontinuous nature of the centromeric and gene position effects have not yet been studied as a single p

116 quences of single gene change independent of position effects; (ii) provide a fine-structure dissecti

117 susceptible to transcriptional silencing and position effects imparted by chromosomal sequences at th

118 latory DNA elements governing expression and position effects, improve our control over the physiolog

119 maternal chromosome may exert a suppressive position effect in cis.

120 Telomeric position effect in Saccharomyces cerevisiae is a chromat

121 effective in reducing silencing chromosomal position effects in a variety of settings.

122 nked by insulator elements are shielded from position effects in Drosophila cells, we tested the abil

123 sulate transgene expression from chromosomal position effects in Drosophila melanogaster.

124 also insulated mini-white transgenes against position effects in Drosophila melanogaster.

125 i, insulated white transgene expression from position effects in Drosophila melanogaster.

126 when it lies outside, and to protect against position effects in Drosophila.

127 e ability of cHS4 to protect transgenes from position effects in mammalian cells.

128 ck promoter-enhancer interactions and reduce position effects in some transgenic animals, we examined

129 in conformational data for the prediction of position effects in the clinical interpretation of non-c

130 r to address the contribution of chromosomal position effects in the context of a fully sequenced gen

131 us control regions (LCRs) generally overcome position effects in transgenes.

132 gene cluster, which has been shown to reduce position effects in transgenic Drosophila.

133 ition-sensitive (A)gamma-globin gene against position effects in transgenic mice.

134 The nature of the position effects involved remains unknown but does not c

135 Protection against position effects involves other properties that appear t

136 tial molecular mechanism responsible for the position effect is discussed.

137 The position effect is evident also in cases where a silent

138 The chromosome 2 position effect is not explained by the NOR2-associated

139 A promising strategy to mitigate position effects is the identification of insulator or b

140 This "position effect" is seen in cases where a mutation creat

141 tive capacity may therefore not arise from a position effect mechanism as has been previously suggest

142 lance Hypothesis is hypothetical because the position effect mechanisms implicated are not proved to

143 e heterochromatic state and makes a telomere positioning effect more likely.

144 e use of genomic approaches for the study of position effect mutations.

145 Both asymmetry and the position effect of H-segment integration disappeared upo

146 However, there was a distinct position effect of the preferred C residues.

147 c expression, they do not shield against the position effects of adjacent chromatin.

148 luciferase assay to systematically quantify position effects of host chromatin and the ability of in

149 To assess directly the position effects of Nor loci on recombination across chr

150 ied as a function of steric, electronic, and position effects of the substituents on the starting Mic

151 e abietenyl intermediate depends more on the positioning effects of the carbocation-diphosphate anion

152 summary, our work unequivocally identifies a position effect on gene targeting in human cells.

153 study, we show that telomeres also impose a position effect on mitotic recombination.

154 Mechanisms for the position effect on mutation frequency are evaluated.

155 nd of particular interest, there is a strong position effect on mutation frequency within the eight-m

156 wever, there is no report regarding telomere position effect on natural telomeric genes in human cell

157 ese deletions are thought to cause FSHD by a position effect on other genes.

158 , we describe the first potential long-range position effect on the expression of TRPS1.

159 the opposite, only monolinguals exhibited a position effect on the late positive component for both

160 Further RNA analysis indicated a modest tRNA position effect on Ty1 transcription.

161 lthough other possibilities exist, including position effects on expression of imprinted genes or tha

162 l and highlight the importance of chromosome position effects on gene expression profiles in bacteria

163 fs between transposon silencing and negative position effects on gene expression.

164 effect of an upstream enhancer and to reduce position effects on mini-white expression in Drosophila

165 Telomere position effects on transcription (TPE, or telomeric sil

166 Thus, unlike position effects on transcription or replication, inhibi

167 c consistency was compromised by chromosomal position effects on vector expression.

168 ng genome, a phenomenon known as chromosomal position effect or CPE.

169 h gene across genomic positions (generalized position effects, or GPEs) may overwhelm differences bet

170 ene and its genomic milieu (lineage-specific position effects, or LSPEs).

171 We term this process TPE-OLD for "telomere position effect over long distances." Our results sugges

172 ted up to 10 Mb from the telomeres (Telomere Position Effect-Over Long Distances [TPE-OLD]).

173 ractions may, in general, be responsible for position effect phenomena and potentially cause many dis

174 te transgene expression resulting in complex position effect phenotypes.

175 ent genes may be differentially sensitive to position effects, reflecting unique interactions between

176 Fruit position effects regarding photosynthetically active rad

177 bloodstream-form T.brucei are restrained by position effects related to their proximity to vsgs or o

178 Because position effects result from the unique chromatin struct

179 he proximal Y short arm, are female due to a position effect resulting in non-expression of Sry ; sex

180 cus control regions) to transgenes overcomes position effect silencing, even within centromeric chrom

181 dependent effect on heterochromatin mediated position-effect silencing.

182 lso not directly attributable to chromosomal position effects, since addition of chromatin insulators

183 These results reveal that the position-effect suppression region of the TCRalpha LCR h

184 n therefore be more sensitive to chromosomal position effects than the rate-determining steps for gen

185 Collectively, these results describe a position effect that downregulates TRPS1 expression as t

186 Chromosomal position effects that eliminated amplification of transg

187 nt genomic locations often varies because of position effects that have been subcategorized as stable

188 Next, to exclude possible position effects that may influence methylation of the i

189 gration displayed either stable or silencing position effects that were dominant over the different e

190 ral modifications at the steroid C-6 and C-7 positions effect the actions of these anesthetic steroid

191 To address how these two positions effect the specificity of beta-lactamase bindi

192 re lengthening and slightly reduced telomere position effect, the cellular level of the Trt1 protein

193 entional transgenesis methods are plagued by position effects: the regulatory environment of genomic

194 Prevention of chromosome position effects, therefore, may be the result of embedd

195 onfirmed as a dominant modifier of telomeric position effect through the use of a genetic test.

196 CR construct tested could not overcome these position effects to confer equal expression at all three

197 One model of telomeric position effect (TPE) in Drosophila melanogaster propose

198 In yeast, telomere position effect (TPE) results in the reversible silencin

199 located near telomeres, termed the telomere position effect (TPE), is well characterized in Saccharo

200 gi including mating type switching, telomere position effect (TPE), silencing of ribosomal DNA, regul

201 ironment at telomeres gives rise to telomere position effect (TPE), the epigenetic silencing of telom

202 s is repressed, a phenomenon termed telomere position effect (TPE).

203 adjacent genes, a phenomenon called telomere position effect (TPE).

204 cking Sir3p, which is essential for telomere position effect (TPE).

205 al silencing through the process of telomere position effect (TPE).

206 es suppress gene expression, called telomere position effect (TPE).

207 ociated sequence (TAS), a source of telomere position effect (TPE).

208 ally repressed, a phenomenon termed telomere position effect (TPE).

209 suppressor of telomeric silencing [telomeric position effect (TPE)].

210 Silencing at telomeres (telomere position effect [TPE]) and the cryptic mating-type loci

211 es are transcriptionally repressed (telomere position effect, TPE).

212 trol as transcriptional repression (telomere position effect; TPE), as mutations in genes essential f

213 tion, the activity in this region suppresses position effect transgene silencing in many tissues.

214 We have investigated both types of position-effect variation by applying our method of tran

215 d HSS3), which is required for prevention of position effect variegation (PEV) in transgenic mice.

216 Position effect variegation (PEV) occurs when a gene is

217 some (Dp(1;f)1187) dramatically increase the position effect variegation (PEV) of a yellow(+) body-co

218 stone H3S10 kinase are strong suppressors of position effect variegation (PEV) of the wm4 allele and

219 d that loss of 8 out of 13 JmjC genes modify position effect variegation (PEV) phenotypes, consistent

220 able gene repression, such as is observed in position effect variegation (PEV) when the Drosophila me

221 Drosophila melanogaster chromosomes exhibit position effect variegation (PEV), a mosaic silencing ch

222 Reduction of roX function suppresses position effect variegation (PEV), revealing functional

223 a PcG gene and mutations in His2Av suppress position effect variegation (PEV), suggesting that this

224 uchromatin and heterochromatin can result in position effect variegation (PEV), the variable expressi

225 as exemplified in Drosophila melanogaster by position effect variegation (PEV).

226 transgene are silenced-a phenomenon known as position effect variegation (PEV).

227 It is a strong dominant suppressor of position effect variegation (PEV).

228 to demonstrate that dLDH and L-2HG influence position effect variegation and DNA methylation, suggest

229 LCRs protect transgenes from position effect variegation and heterochromatinization a

230 natural or synthetic PATCs are resistant to position effect variegation and stochastic silencing in

231 of silencers in trans or by the spreading of position effect variegation from rearrangements having h

232 erature, such as vernalization in plants and position effect variegation in animals.

233 Similarities between position effect variegation in Drosophila and gene silen

234 to the postulated role of this DNA repeat in position effect variegation in facio- scapulohumeral mus

235 as been described in diverse systems such as position effect variegation in insects, silencing near y

236 y nor qualitatively affected by modifiers of position effect variegation including the Y chromosome,

237 r full function of the AE1 promoter and that position effect variegation is associated with RNA trans

238 Position effect variegation may be considered an abnorma

239 Studies of position effect variegation suggest that promoters of he

240 is, and it decreased (but did not alleviate) position effect variegation within the expressing cell t

241 By using a combination of two modifiers of position effect variegation, adding an extra Y chromosom

242 overed in HP2 act as dominant suppressors of position effect variegation, confirming a role in hetero

243 presence of Su(z)12, a strong suppressor of position effect variegation, in PRC2 suggests that PRC2

244 Mutations in HP2 can suppress position effect variegation, indicating a role in gene s

245 tation in Drosophila PR-Set7 that suppresses position effect variegation, indicating that PR-Set7 ind

246 tic DNA instability described here underlies position effect variegation, molds the structure of poly

247 Rga also acts as a suppressor of position effect variegation, suggesting that a possible

248 Mutations in Nap-1 are shown to suppress position effect variegation, suggesting that Nap-1 funct

249 IR2 mutations were recently shown to perturb position effect variegation, suggesting that the role of

250 emplified by piRNAs (piwi-interacting RNAs), position effect variegation, X-chromosome inactivation,

251 ression, a heterochromatin phenomenon called position effect variegation.

252 ncer (TE) and a region that protects against position effect variegation.

253 netic gene silencing classically observed as position effect variegation.

254 reminiscent of, but clearly different from, position effect variegation.

255 l involvement of the P0 protein in modifying position effect variegation.

256 s not express p185 are strong suppressors of position effect variegation.

257 act in these domains, as shown by monitoring position effect variegation.

258 d by suppression of heterochromatin-mediated position effect variegation.

259 allelic to Lighten-up, a known suppressor of position effect variegation.

260 sed as the explanation for such phenomena as position-effect variegation (PEV) and control of segment

261 This would be similar to a loss of position-effect variegation (PEV) in Drosophila.

262 gaster, heterochromatin-induced silencing or position-effect variegation (PEV) of a reporter gene has

263 BID expression also enhances position-effect variegation (PEV) of the w(m4h) allele a

264 osome in regulating rRNA gene transcription, position-effect variegation (PEV), and the link among rD

265 iable, fertile, and recessive suppressors of position-effect variegation (PEV), indicating that, as i

266 eterochromatin and regulates heterochromatin position-effect variegation (PEV), organization of repet

267 in an understanding of aneuploid syndromes, position-effect variegation (PEV), quantitative traits,

268 , we found that dhtt acts as a suppressor of position-effect variegation (PEV), suggesting that it in

269 antagonistic and counterbalancing effect on position-effect variegation (PEV).

270 n/euchromatin at three distinct loci showing position-effect variegation (PEV).

271 t required for survival and does not control position-effect variegation (PEV).

272 Telomere-associated position-effect variegation (TPEV) in budding yeast has

273 ear telomeres is attenuated through telomere position-effect variegation (TPEV).

274 ls of retinal degeneration and mechanisms of position-effect variegation and demonstrate the utility

275 The lawc gene behaves as an enhancer of position-effect variegation and interacts genetically wi

276 gmentation patterns similar to those seen in position-effect variegation and yet most inserts were in

277 L-1 histone H3S10 kinase act as enhancers of position-effect variegation at pericentric sites whereas

278 in Drosophila melanogaster for modifiers of position-effect variegation have revealed the basis of m

279 letes H3K9 methylation levels and suppresses position-effect variegation in various Drosophila tissue

280 Position-effect variegation of the w(m4h) allele and dif

281 riptional repression of nearby marker genes (position-effect variegation or silencing).

282 ivity to DNA-damaging agents, suppression of position-effect variegation, and female sterility in whi

283 a variety of biological processes including position-effect variegation, heterochromatin formation a

284 tic screens that relied on mosaic silencing (position-effect variegation, or PEV) of the yellow gene

285 effect of both Su(var)3-9 and Su(var)2-5 on position-effect variegation, providing evidence that a f

286 With position-effect variegation, similar responses were foun

287 acts as both an enhancer and a suppressor of position-effect variegation.

288 to further methylation, suggests a model for position-effect variegation.

289 cus, initially identified as a Suppressor of Position-Effect Variegation.

290 lls, an effect that is probably analogous to position-effect variegation.

291 nearby gene in cis, a hallmark of classical position-effect variegation.

292 9me3, and is a classic genetic suppressor of position-effect variegation.

293 yed by nuclear organization in cis and trans position-effect variegation.

294 This position effect was investigated by placing the mutant l

295 stand the role of telomere structure in this position effect, we have developed an assay to distingui

296 To eliminate position effects, we generated and analyzed pairs of sis

297 These eye position effects were appropriate to maintain coding in

298 These position effects were strongly dependent on the orientat

299 in at the local level, they become immune to position effects which affect expression of smaller tran

300 me, these vectors often suffer from profound position effects, with vector silencing observed in vitr