ライフサイエンスコーパス: pregenomic RNA

1 ated sensing of the 5'-epsilon region of HBV pregenomic RNA.

2 otein and the DNA polymerase and also is the pregenomic RNA.

3 mmediately prior to the encapsidation of the pregenomic RNA.

4 d the phenotype of enhanced encapsidation of pregenomic RNA.

5 ar relaxed circular double-stranded DNA, and pregenomic RNA.

6 number of nucleotides from the 5' end of the pregenomic RNA.

7 most likely binding of the polymerase to the pregenomic RNA.

8 reverse transcriptase ("polymerase") and the pregenomic RNA.

9 prevent the proper encapsidation of the HBV pregenomic RNA.

10 riptase binds cotranslationally to the viral pregenomic RNA.

11 3 kb of full-length in vitro-transcribed HBV pregenomic RNA.

12 tide and an RNA segment, epsilon, present on pregenomic RNA.

13 ly repressed synthesis of both the pre-C and pregenomic RNAs.

14 This clone, named HDE1, expresses a chimeric pregenomic RNA, a chimeric polymerase (P) protein, and a

15 ends upon cis-acting elements present in its pregenomic RNA and a trans-acting protein (P6) which is

16 in dimer bound and encapsidated both the HBV pregenomic RNA and heterologous RNA with high levels of

17 apy, as are lower intrahepatic levels of HBV pregenomic RNA and pre-activated host immune responses.

18 rocess leading to (1) the packaging of viral pregenomic RNA and reverse transcriptase into nucleocaps

19 es support transcription of the 3.5-kb viral pregenomic RNA and subsequent viral DNA synthesis by rev

20 Likewise, no activation of synthesis of pregenomic RNA and viral DNA by PPARalpha-RXRalpha was o

21 significantly higher levels of synthesis of pregenomic RNA and viral DNA than wild-type HBV in coexp

22 gonized each other's effects on synthesis of pregenomic RNA and viral DNA when they were co-overexpre

23 ntial target sites were found within the HBV pregenomic RNA, and 17 sites conserved in all four subty

24 mediate DNA, covalently closed circular DNA, pregenomic RNA, and the percentage of WHV core antigen-p

25 trical capsid (built of core protein), viral pregenomic RNA, and viral reverse transcriptase.

26 first 74 nucleotides of the 7,954-nucleotide pregenomic RNA appears to be essential for P6 to transac

27 taining the viral single-stranded DNA or the pregenomic RNA are incompetent for either envelopment or

28 nerated by the final RNase H cleavage of the pregenomic RNA at an 11 nt sequence called DR1 during th

29 site lesions produced a modest reduction of pregenomic RNA but had no impact on viral DNA synthesis.

30 re-than-10-fold increase in synthesis of the pregenomic RNA but to only a 2- to 3-fold increase in sy

31 hepadnaviruses is reverse transcribed from a pregenomic RNA by a viral polymerase (Pol) harboring bot

32 ignal (the packaging signal, epsilon) on the pregenomic RNA by the viral reverse transcriptase (RT).

33 e preferential reduction in the level of the pregenomic RNA compared with that of precore RNA.

34 tis B virus (HBV) replication by eliminating pregenomic RNA containing viral capsids from the hepatoc

35 IFN-alpha/beta or IFN-gamma, which eliminate pregenomic RNA-containing capsids from the cells as they

36 The pregenomic RNA directs replication of the hepatitis B vi

37 HNF4 and TR2) or activated synthesis of the pregenomic RNA (e.g., PPARgamma-RXRalpha), other members

38 isense RNA was found to reduce the amount of pregenomic RNA encapsidated into core particles as a mol

39 Here we study the thermodynamic basis of the pregenomic RNA encapsidation in human Hepatitis B virus

40 ng the F501L substitution showed a decreased pregenomic RNA encapsidation level, suggesting that the

41 to affect both core protein translation and pregenomic RNA encapsidation.

42 The pregenomic RNAs encode both the nucleocapsid protein and

43 However, the pregenomic RNA, endogenous polymerase activity, and core

44 ability to generate the terminally redundant pregenomic RNA essential for genome replication.

45 cells, lesions in the HNF-1 site inactivated pregenomic RNA expression and viral reverse transcriptio

46 volves the transcription of the 3.5-kb viral pregenomic RNA, followed by its reverse transcription in

47 g an inducible viral genome implicated viral pregenomic RNA in apolipoprotein mRNA reduction.

48 II is primarily devoted to the regulation of pregenomic RNA in WHV, (ii) HNF-1 is essential for EnII

49 atitis B viral DNA genome proceeds through a pregenomic RNA intermediate.

50 a result of increased viral encapsidation of pregenomic RNA into the core particles.

51 The duck hepatitis B virus (DHBV) pregenomic RNA is a bicistronic mRNA encoding the core a

52 The pregenomic RNA is the template for reverse transcription

53 ts indicated that synthesis of the pre-C and pregenomic RNAs is directed by two distinct promoters an

54 terized by a more efficient encapsidation of pregenomic RNA leading to highly enhanced replication.

55 Lower intrahepatic HBV pregenomic RNA levels and 25 predictive genes were ident

56 iRNA, Northern blot analysis showed that HBV pregenomic RNA levels were decreased by 72%, and levels

57 ld repression of synthesis of both pre-C and pregenomic RNAs mediated through either NRRE(preC) or NR

58 atial regulation of the genes present on the pregenomic RNA of FMV.

59 The epsilon stem-loop at the 5' end of the pregenomic RNA of the hepatitis B viruses is both the pr

60 Synthesis of the pre-C and pregenomic RNAs of human hepatitis B virus (HBV) is dire

61 In addition to packaging viral pregenomic RNA (pgRNA) and DNA polymerase complex into n

62 ously, was performed to measure encapsidated pregenomic RNA (pgRNA) and minus-strand DNA synthesized

63 The capsid protein (Cp) packages the viral pregenomic RNA (pgRNA) and polymerase to form the HBV co

64 plication by decreasing the transcription of pregenomic RNA (pgRNA) and subgenomic RNA from the HBV c

65 otein specifically encapsidates a complex of pregenomic RNA (pgRNA) and viral polymerase; it has been

66 V) capsid proteins (Cps) assemble around the pregenomic RNA (pgRNA) and viral reverse transcriptase (

67 ignal (the packaging signal, epsilon) on the pregenomic RNA (pgRNA) by the viral reverse transcriptas

68 NA genome through reverse transcription of a pregenomic RNA (pgRNA) by using a multifunctional polyme

69 NA genome through reverse transcription of a pregenomic RNA (pgRNA) by using a multifunctional polyme

70 terminal regions of hepatitis B virus (HBV) pregenomic RNA (pgRNA) harbors sites governing many esse

71 visualized core (Cp), polymerase (Pol), and pregenomic RNA (pgRNA) in infected cells.

72 Packaging of hepadnavirus pregenomic RNA (pgRNA) into capsids, or encapsidation, r

73 s B virus (HBV) begins with packaging of the pregenomic RNA (pgRNA) into immature nucleocapsids (NC),

74 o identify other cis-acting sequences on the pregenomic RNA (pgRNA) involved in the synthesis of minu

75 The pregenomic RNA (pgRNA) of hepadnaviruses is packaged int

76 The pregenomic RNA (pgRNA) of hepatitis B virus (HBV) serves

77 T-epsilon interaction with those for in vivo pregenomic RNA (pgRNA) packaging clearly indicated that

78 Pregenomic RNA (pgRNA) plays two major roles in the hepa

79 ther analysis showed that HNF6 reduced viral pregenomic RNA (pgRNA) posttranscriptionally via acceler

80 nt in multiple copies in and adjacent to the pregenomic RNA (pgRNA) terminal redundancy, that were sp

81 required for efficient encapsidation of its pregenomic RNA (pgRNA), epsilon and region II.

82 In addition to the 3.5-kb pregenomic RNA (pgRNA), the mutant preferentially encaps

83 ere we show that the pool of cytoplasmic HBV pregenomic RNA (pgRNA)-containing capsids is reduced 10-

84 ds dose dependently inhibit the formation of pregenomic RNA (pgRNA)-containing nucleocapsids of HBV b

85 quired for specifically binding to the viral pregenomic RNA (pgRNA).

86 at replicates via reverse transcription of a pregenomic RNA (pgRNA).

87 the CTD does contribute to encapsidation of pregenomic RNA (pgRNA).

88 transcription of an RNA intermediate termed pregenomic RNA (pgRNA).

89 n icosahedral capsid that packages the viral pregenomic RNA (pgRNA).

90 overlaps the core (C) ORF on the bicistronic pregenomic RNA (pgRNA).

91 te this DNA through an RNA intermediate (the pregenomic RNA, pgRNA) by reverse transcription.

92 d to the downregulation of viral protein and pregenomic RNA production.

93 r II (EnII) is located upstream of the major pregenomic RNA promoter and is thought to play an import

94 ages corresponding to encapsidation of viral pregenomic RNA, reverse transcription, and restriction t

95 (nucleotides 1772 to 1859) located 5' to the pregenomic RNA start site.

96 The pregenomic RNA structure and the capsid stoichiometry im

97 This pregenomic RNA subsequently serves as the template for t

98 ective ligands led to a fourfold increase in pregenomic RNA synthesis and a four- to fivefold increas

99 heterodimer support hepatitis B virus (HBV) pregenomic RNA synthesis and viral replication in nonhep

100 HNF3 and HNF4 synergistically activate DHBV pregenomic RNA synthesis and viral replication.

101 r receptors are a primary determinant of HBV pregenomic RNA synthesis and, hence, viral replication.

102 reC,) played the major role in activation of pregenomic RNA synthesis by HNF4alpha.

103 lication can occur in nonhepatoma cells when pregenomic RNA synthesis from viral DNA is activated by

104 vations indicate that HBV transcription, and pregenomic RNA synthesis in particular, is regulated by

105 As HBV pregenomic RNA synthesis is primarily believed to be reg

106 plication in nonhepatic cells by controlling pregenomic RNA synthesis, indicating these liver-enriche

107 ignal (the packaging signal, epsilon) on the pregenomic RNA template by the viral reverse transcripta

108 he RT and an RNA signal located on the viral pregenomic RNA, termed epsilon, and is initiated through

109 m position on a bicistronic mRNA, called the pregenomic RNA, through a poorly characterized ribosomal

110 reverse transcriptase, which pins the viral pregenomic RNA to the capsid inner surface.

111 ed mutations resulted in a minor increase of pregenomic RNA transcription (two- to threefold) and a m

112 transcription factors known to support viral pregenomic RNA transcription and replication.

113 In contrast, HNF4 supports higher levels of pregenomic RNA transcription from the variant than from

114 alpha heterodimers support higher levels of pregenomic RNA transcription from the wild-type than fro

115 tions resulted in only a twofold increase in pregenomic RNA transcription.

116 in encapsidation was largely independent of pregenomic RNA transcription.

117 irect competition assays, no specificity for pregenomic RNA was observed.

118 Synthesis of the pre-C and pregenomic RNAs was affected both in transfected hepatom

119 HBV DNA replication is driven by pregenomic RNA, which is controlled by core promoter (CP