ライフサイエンスコーパス: preinitiation

1 assembly and the regulation of transcription preinitiation.

2 mapped movements of divalent cations during preinitiation.

3 initiation factor (eIF) 2 and eIF3, to form preinitiation 48S ribosomal complexes that subsequently

4 se kinases, which are thought to function in preinitiation and elongation.

5 tion factors eIF1, eIF1A and eIF3 in the 40S preinitiation complex (40S.eIF1.eIF1A.eIF3.Met-tRNA(i).e

6 lity, the hydrolysis of GTP bound to the 40S preinitiation complex (40S.Met-tRNA(i).eIF2.GTP), promot

7 g eukaryotic translation initiation, the 43S preinitiation complex (43S PIC), consisting of the 40S r

8 Dissociation of eIF1 from the preinitiation complex (PIC) allows release of phosphate

9 omain (eIF5-CTD) directly links eIF4G to the preinitiation complex (PIC) and enhances mRNA binding.

10 of melted DNA separately associated with the preinitiation complex (PIC) and the adjacent paused comp

11 anslation initiation factor eIF1A stimulates preinitiation complex (PIC) assembly and scanning, but t

12 ription factor TFIIB plays a central role in preinitiation complex (PIC) assembly and the recruitment

13 SIR-mediated silencing is permissive to both preinitiation complex (PIC) assembly and transcription i

14 e GAL1 UAS, and facilitates formation of the preinitiation complex (PIC) assembly at the GAL1 promote

15 he steps leading to chromatin remodeling and preinitiation complex (PIC) assembly differ significantl

16 A "step-wise" preinitiation complex (PIC) assembly model has been sugg

17 specific activator NF-E2 to the promoter and preinitiation complex (PIC) assembly occur only after di

18 (PRC1) inhibits activated RNA polymerase II preinitiation complex (PIC) assembly using immobilized H

19 FIIF are both required for RNA polymerase II preinitiation complex (PIC) assembly, but their roles at

20 he structure of transcription factors (TFs), Preinitiation Complex (PIC) assembly, RNA polymerase II

21 ween VP16-mediated chromatin acetylation and preinitiation complex (PIC) assembly.

22 nscription factor TFIID, which might promote preinitiation complex (PIC) assembly.

23 anscription initiation and play key roles in preinitiation complex (PIC) assembly.

24 events PESE-dependent Pol II recruitment and preinitiation complex (PIC) assembly.

25 ies of highly regulated steps: assembly of a preinitiation complex (PIC) at the promoter nucleated by

26 s of highly regulated steps: assembly of the preinitiation complex (PIC) at the promoter, initiation,

27 vating protein complex, form a transcription preinitiation complex (PIC) at the spliced leader (SL) R

28 olving mRNA secondary structures that impede preinitiation complex (PIC) attachment to mRNA or scanni

29 The eukaryotic 43S preinitiation complex (PIC) bearing Met-tRNAi(Met) in a

30 defective for interaction with polymerase II preinitiation complex (PIC) components and other regulat

31 The resulting complete pol II transcription preinitiation complex (PIC) contained equimolar amounts

32 steps in transcription initiation including preinitiation complex (PIC) formation and start site sel

33 ans is regulated in several steps, including preinitiation complex (PIC) formation, initiation, Pol I

34 III dependent transcription before and after preinitiation complex (PIC) formation.

35 s p53-dependent transcription by stimulating preinitiation complex (PIC) formation; (2) H3K4me3, thro

36 scription commences with the assembly of the Preinitiation Complex (PIC) from a plethora of proteins

37 work implicated eIF5 in rearrangement of the preinitiation complex (PIC) from an open, scanning confo

38 ensus motif for the positioning of the human preinitiation complex (PIC) has been identified.

39 complexes remove the -1 nucleosome after the preinitiation complex (PIC) has partially assembled, but

40 ferences of the key components of eukaryotic preinitiation complex (PIC) have been recently measured

41 promoting ATP-dependent dissociation of the preinitiation complex (PIC) into the Scaffold complex.

42 don in an mRNA by the eukaryotic translation preinitiation complex (PIC) is essential for proper gene

43 The translation preinitiation complex (PIC) is thought to assume an open

44 1), eIF1A, eIF3, and eIF5, and the resulting preinitiation complex (PIC) joins the 5' end of mRNA pre

45 The formation of a stable 43 S preinitiation complex (PIC) must occur to enable success

46 that a reduced affinity of eIF3j for the 43S preinitiation complex (PIC) occurs on eIF4F-dependent mR

47 The assembly of the preinitiation complex (PIC) occurs upstream of the +1 nu

48 ns with the assembly of an RNA polymerase II preinitiation complex (PIC) on the promoter.

49 oactivators are required for assembly of the preinitiation complex (PIC) or for subsequent steps in t

50 ires the assembly at the promoter of a large preinitiation complex (PIC) that includes RNA polymerase

51 inately recruit RNA polymerase II and form a preinitiation complex (PIC) to activate MyoD transcripti

52 as been implicated in attachment of the 43 S preinitiation complex (PIC) to mRNAs and scanning to the

53 ve reconstituted mRNA recruitment to the 43S preinitiation complex (PIC) using purified S. cerevisiae

54 e of a 33-protein, 1.5-MDa RNA polymerase II preinitiation complex (PIC) was determined by cryo-EM an

55 onents of a large RNA polymerase II (Pol II) preinitiation complex (PIC) were distinguished from a ba

56 nucleates the assembly of the transcription preinitiation complex (PIC), and although TBP can bind p

57 x and its role, along with components of the preinitiation complex (PIC), in histone eviction at indu

58 s H2A and H2B, Nap1p, and a component of the preinitiation complex (PIC), TBP.

59 formation and function of the promoter-bound preinitiation complex (PIC), which consists of RNA polym

60 ted steps in the assembly of a transcription preinitiation complex (PIC).

61 the RNA polymerase II (Pol II) transcription preinitiation complex (PIC).

62 with RNA polymerase (Pol) II as part of the preinitiation complex (PIC).

63 on domain on Pol II within the transcription preinitiation complex (PIC).

64 inhibits the assembly and the function of a preinitiation complex (PIC).

65 ption factors and promoter DNA in a 'closed' preinitiation complex (PIC); unwinding of about 15 base

66 tiation of DNA synthesis from each origin, a preinitiation complex (pre-IC) containing Cdc45 and othe

67 and a core component of the DNA replication preinitiation complex (pre-IC), and that the TICRR-TopBP

68 scription by blocking the recruitment of the preinitiation complex (RNA polymerase II and TFIIB) to t

69 oter recruitment of poised RNA polymerase II preinitiation complex (RNAPII PIC), which enhances futur

70 s that a structural rearrangement in the 43S preinitiation complex activates it to become fully compe

71 Phosphorylation of components of the preinitiation complex activates replication and prevents

72 Thus, the eIF3 preinitiation complex acts as a scaffold to coordinate a

73 c1Delta mutation affects the assembly of the preinitiation complex after osmotic shock, it does not a

74 kinetic dissection of the transition from a preinitiation complex after start codon recognition to t

75 To study TBP functions in preinitiation complex and other complexes, we generated

76 gene-specific transcription factors with the preinitiation complex and RNA polymerase II.

77 f mRNA and stimulates recruitment of the 43S preinitiation complex and subsequent scanning to the ini

78 anding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and

79 4me3 that leads to assembly of transcription preinitiation complex and transcriptional activation.

80 , for Mediator-dependent assembly of a basal preinitiation complex and, more important, identify a st

81 core promoter of RNR3 is sufficient to drive preinitiation complex assembly and activate transcriptio

82 0S binding of eIF3 and is needed for optimal preinitiation complex assembly and AUG recognition in vi

83 EAT domain is a critical nucleation core for preinitiation complex assembly and function.

84 ontrol global gene expression in eukaryotes: preinitiation complex assembly and polymerase pausing.

85 re, a TFIIB-related protein is implicated in preinitiation complex assembly and postpolymerase recrui

86 ations of these results for the mechanism of preinitiation complex assembly and promoter melting.

87 y by the activator Gcn4p but is dependent on preinitiation complex assembly and Ser5 carboxy-terminal

88 oter chromatin remodeling from transcription preinitiation complex assembly and suggest the existence

89 sis coupled with native gel electrophoresis, preinitiation complex assembly assays, and translation i

90 8p and H3-K4 methylation are dispensable for preinitiation complex assembly at the core promoters of

91 ability of chromatin-bound Gcn4 to stimulate preinitiation complex assembly at the promoter.

92 inct mechanisms for ribosome recruitment and preinitiation complex assembly between the two processes

93 can be used to learn how different modes of preinitiation complex assembly impact transcriptional ac

94 IIB derivative is able to support high order preinitiation complex assembly in the absence of an acti

95 However, it is unknown how preinitiation complex assembly is coordinated with chrom

96 required for activators to stimulate Pol II preinitiation complex assembly on an associated promoter

97 with the MED23 Mediator subunit, stimulating preinitiation complex assembly on early viral promoters

98 om the TAF1-TFIID complex upon completion of preinitiation complex assembly, allowing transcription t

99 ated GR attenuated ISGF3 promoter occupancy, preinitiation complex assembly, and ISG expression.

100 t Mdm30p is dispensable for formation of the preinitiation complex assembly, association of elongatin

101 inhibiting an essential function of TFIIF in preinitiation complex assembly, but also that Mediator c

102 rent steps in transcription before and after preinitiation complex assembly.

103 sferase-independent function at the level of preinitiation complex assembly.

104 ion elongation factor TFIIS functions during preinitiation complex assembly.

105 h, which allows TFIID binding and subsequent preinitiation complex assembly.

106 te Pol II initiation at a step subsequent to preinitiation complex assembly.

107 MFC), an important intermediate for the 43 S preinitiation complex assembly.

108 T1/Cdk9 inhibits PGC-1 promoter activity and preinitiation complex assembly.

109 ding to core promoter elements and directing preinitiation complex assembly.

110 lucosaminidase) blocked transcription during preinitiation complex assembly.

111 Consistent with this finding, Mediator and preinitiation complex association with SAGA-dependent pr

112 e present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 A resolution with initiator

113 on network is essential for formation of the preinitiation complex at the core promoter to initiate t

114 olymerase II, nucleating the assembly of the preinitiation complex at the transcription start site.

115 ation of most eukaryotic mRNAs occurs when a preinitiation complex binds to the 5' cap, scans the mRN

116 s bind immediately downstream of the Pol III preinitiation complex but are not required for Pol III r

117 ire the preloading of RNA polymerase II or a preinitiation complex but instead depends upon the Gcn5

118 of eIF1 was identified, interfaces to other preinitiation complex components and their relevance to

119 caffold for the dynamic associations of many preinitiation complex components, including the growth-r

120 et out to fine-map a small viral replication preinitiation complex composed of two protein dimers bou

121 e of mediating assembly of the transcription preinitiation complex containing RNA polymerase II.

122 iation in Archaea requires the assembly of a preinitiation complex containing the TATA- box binding p

123 nt in pol III recruitment, but the resulting preinitiation complex does not open the promoter.

124 gly, the initiation codon recognition by 43S preinitiation complex during EXT2 translation is suppres

125 of coregulators, leading to the formation of preinitiation complex followed by elongation.

126 able to scan template DNA downstream of the preinitiation complex for acceptable start sites.

127 IID (TFIID), which nucleates assembly of the preinitiation complex for transcription by RNA polymeras

128 reagents, their effect on transcription and preinitiation complex formation and discuss their potent

129 ter escape are linked to stronger effects on preinitiation complex formation and transcription, sugge

130 on it alters chromatin structure to increase preinitiation complex formation and transcription.

131 omote dephosphorylation of Ser5 to stimulate preinitiation complex formation and, later, to inhibit e

132 ts with promoter conformation and downstream preinitiation complex formation and/or function.

133 associated factors (TAFs), is essential for preinitiation complex formation at ribosomal RNA gene pr

134 rved cis elements, the TFIID complex directs preinitiation complex formation at specific sites in cor

135 e context-dependent Saccharomyces cerevisiae preinitiation complex formation at the HIS4 promoter rec

136 egative regulation of transcription, correct preinitiation complex formation in basal and activated t

137 Furthermore, Tup1 repressed RNR3 and blocked preinitiation complex formation in the Deltaisw2 mutant,

138 ubunit, in Pol I recruitment and, therefore, preinitiation complex formation in vivo.

139 opose a model of stochastic enhanceosome and preinitiation complex formation that incorporates transc

140 anning sequences downstream from the site of preinitiation complex formation, a process that involves

141 ARG1 is independent of the TATA element and preinitiation complex formation, whereas efficient recru

142 osmoresponse gene transcription by enhancing preinitiation complex formation.

143 histone acetylation) at a step subsequent to preinitiation complex formation.

144 alterations and subsequent RNA polymerase II preinitiation complex formation.

145 ily blocks steps downstream of transcription preinitiation complex formation.

146 of TATA box genes appear to be controlled at preinitiation complex formation.

147 arts of the IRES RNA change structure as the preinitiation complex forms.

148 scription factor IIB and dissociation of the preinitiation complex from the major promoter.

149 nesis requires SWI/SNF enzymes to facilitate preinitiation complex function.

150 SNF affects recruitment of components of the preinitiation complex in a promoter-specific manner to m

151 air GCN4 translational control by disrupting preinitiation complex interactions.

152 onal changes that mark the transition of 30S preinitiation complex into elongation competent 70S comp

153 n by BRCA1 is that the ubiquitination of the preinitiation complex is not targeting proteins for degr

154 se that the overall structure of the RNAP II preinitiation complex is similar in all eukaryotes and i

155 TOR), and appears to represent an incomplete preinitiation complex lacking several subunits.

156 lating the assembly of the 48S translational preinitiation complex mediated by the p27 IRES element.

157 d the AdMLP suggests that Rep78/68 alter the preinitiation complex of RNA polymerase II transcription

158 ation of mda-7/IL-24 mRNA, activation of the preinitiation complex of the translational machinery in

159 he other factors necessary for assembly of a preinitiation complex on naked DNA.

160 cription by interfering with assembly of the preinitiation complex or by blocking transcription initi

161 regulate later stages in the assembly of the preinitiation complex or facilitate multiple rounds of p

162 he involvement of MED25 for fully functional preinitiation complex recruitment and transcriptional ou

163 nd eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recog

164 rred, and the activators recruited a partial preinitiation complex that included RNA polymerase II.

165 slation initiation in eukaryotes occurs in a preinitiation complex that includes small ribosomal subu

166 dues of eIF1 disrupts a critical link to the preinitiation complex that suppresses eIF1 release befor

167 information for the papillomavirus E1E2-ori preinitiation complex that would otherwise have been har

168 .eIF2.GTP) and the subsequent binding of the preinitiation complex to eIF4F bound at the 5'-cap struc

169 ription by inhibiting the recruitment of the preinitiation complex to the c-jun promoter.

170 is a central component of the transcription preinitiation complex, and its occupancy at a promoter i

171 binding to Adr1 at promoters that contain a preinitiation complex, demonstrating that Bmh-mediated i

172 TFEalpha/beta stabilises the preinitiation complex, enhances DNA melting, and stimula

173 initiation involves the assembly of the 48S preinitiation complex, followed by joining of the 60S ri

174 neral transcription factors that make up the preinitiation complex, including Pol II, but there was n

175 tial component of the RNA polymerase (pol) I preinitiation complex, is unclear.

176 roteins, but not the conventional autophagic preinitiation complex, or adaptor protein-3 (AP-3).

177 BRCA1 ubiquitinates the transcriptional preinitiation complex, preventing stable association of

178 s impair the integrity of scanning-competent preinitiation complex, thereby altering the 40 S subunit

179 e the interaction with the components of the preinitiation complex, thereby inhibiting its function a

180 method to analyze a yeast RNA polymerase II preinitiation complex, we identified a new 8-kDa protein

181 of EIF1AX, a component of the translational preinitiation complex, were markedly enriched in PDTCs a

182 lymerase II (Pol II) with the promoter-bound preinitiation complex, whereas Brf1 and Brf2 are involve

183 ly bind to the 40S subunit, yielding the 43S preinitiation complex, which is ready to attach to messe

184 factor 3f, a repressive component in the 43S preinitiation complex.

185 en complex results in destabilization of the preinitiation complex.

186 that it involves alterations within the 48S preinitiation complex.

187 es and AUG act coordinately to stabilize the preinitiation complex.

188 binding protein and TFIID, components of the preinitiation complex.

189 hat modulate the binding of mRNA to the 43 S preinitiation complex.

190 e II holoenzyme and DNA, for assembly of the preinitiation complex.

191 ritical for assembly of TFIID and the pol II preinitiation complex.

192 directed reorganization in a transcriptional preinitiation complex.

193 n to direct appropriate assembly of the URA1 preinitiation complex.

194 ongation, or by the inability to establish a preinitiation complex.

195 osphorylation of ribosomal protein S6 on the preinitiation complex.

196 ion of the RNA polymerase II transcriptional preinitiation complex.

197 ormation of the RNA polymerase II-containing preinitiation complex.

198 D initiates formation of the transcriptional preinitiation complex.

199 AUG-dependent dissociation of eIF1 from the preinitiation complex.

200 g eIF5-promoted hydrolysis of GTP in the 40S preinitiation complex.

201 s crucial for the assembly of the eukaryotic preinitiation complex.

202 is is released upon AUG selection by the 40S preinitiation complex.

203 mitting transcriptional activators to form a preinitiation complex.

204 complex and RNA polymerase II to create the preinitiation complex.

205 embly of the RNA polymerase II transcription preinitiation complex.

206 itical for the production of functional 40 S preinitiation complex.

207 R079c-a, as a potential new component of the preinitiation complex.

208 or-P.5 was added after pol II entry into the preinitiation complex.

209 -Rrn3 complex to the rDNA or stabilizing the preinitiation complex.

210 ing rapid promoter escape of Pol II from the preinitiation complex.

211 t to the closed conformation in the scanning preinitiation complex.

212 that required Beclin1, but not the autophagy preinitiation complex.

213 ion initiation begins with assembly of a 43S preinitiation complex.

214 own that it is a functional component of the preinitiation complex.

215 oprecipitated, and OGT is a component of the preinitiation complex.

216 e context of a fully assembled transcription preinitiation complex.

217 polymerase II occurs during assembly of the preinitiation complex.

218 preliminary picture of the RNA polymerase II preinitiation complex.

219 of the mRNA-unwinding machinery to the 43 S preinitiation complex.

220 g transcriptional activators and facilitates preinitiation-complex assembly and transcription.

221 ge number of promoters assemble into partial preinitiation complexes (partial PICs), containing TFIIA

222 if32 CTD that impair mRNA recruitment by 43S preinitiation complexes (PICs) and confer phenotypes ind

223 o determine how Mot1 affects the assembly of preinitiation complexes (PICs) at Mot1-controlled promot

224 manner suggesting incomplete assembly of 48S preinitiation complexes (PICs) at upstream AUG codons in

225 bly of RNA polymerase (Pol) II transcription preinitiation complexes (PICs) have been well establishe

226 eomic analysis of RNA polymerase II (RNApII) preinitiation complexes (PICs) identified Sub1 and the r

227 However, pol II preinitiation complexes (PICs) lose the ability to synth

228 The accumulation of stalled translation preinitiation complexes (PICs) mediates the condensation

229 (MuDPIT) and immunoblot analyses of purified preinitiation complexes (PICs) revealed the recruitment

230 n by facilitating the recruitment of RNAP II preinitiation complexes (PICs) to the promoter regions o

231 face that is required for rapid formation of preinitiation complexes (PICs) was identified on the N-t

232 apid, TATA box-dependent assembly of RNAP II preinitiation complexes (PICs), but permits few rounds o

233 rprisingly, when Gdown1 is added to complete preinitiation complexes (PICs), it does not inhibit init

234 isolated that affect either the assembly of preinitiation complexes (PICs), scanning for AUG, or bot

235 ociation and P(i) release from reconstituted preinitiation complexes (PICs), whereas a hyperaccuracy

236 ngation, but such information is lacking for preinitiation complexes (PICs).

237 ometric screen of RNA polymerase II (Pol II) preinitiation complexes (PICs).

238 complex [TC] with eIF2-GTP) to reconstituted preinitiation complexes (PICs).

239 P1-NTD is required for efficient assembly of preinitiation complexes and also regulates the selection

240 3j/HCR1 remains associated with the scanning preinitiation complexes and does not dissociate from the

241 -a is a bona fide component of polymerase II preinitiation complexes and investigate its role in tran

242 ble binding of at least some mRNAs to native preinitiation complexes and that eIF4G has a rate-limiti

243 a model whereby BRCA1 stabilizes productive preinitiation complexes and thus stimulates transcriptio

244 apoptosis, the assembly of RNA polymerase II preinitiation complexes and WNT signalling.

245 hich is thought to stabilize properly formed preinitiation complexes at the correct start codon.

246 CDK8 module are specifically recruited into preinitiation complexes at the TR target gene type I dei

247 like unexpressed genes without transcription-preinitiation complexes at their promoters, expressed ge

248 rug influences the assembly and stability of preinitiation complexes by targeting the interaction bet

249 This suggests that functional preinitiation complexes can contain Mot1 instead of TFII

250 First, 43S preinitiation complexes comprising 40S ribosomal subunit

251 nslation initiation factor (eIF)3 enable 43S preinitiation complexes containing eIF3 and eIF2-GTP-Met

252 omoters, expressed genes or genes containing preinitiation complexes exhibit characteristic nucleosom

253 The pathways by which preinitiation complexes form, and how this impacts trans

254 important role for RLI1 in assembly of 43 S preinitiation complexes in vivo.

255 ng the formation of stable RNA polymerase II preinitiation complexes leading to transcription initiat

256 erentially affects the assembly of ribosomal preinitiation complexes on different cellular and viral

257 PSEA, DmSNAPc establishes RNA polymerase II preinitiation complexes on U1 to U5 promoters but RNA po

258 on U1 to U5 promoters but RNA polymerase III preinitiation complexes on U6 promoters.

259 However, RNA pol III bound to preinitiation complexes or in elongation complexes is pr

260 se TBP derivatives in isolated transcription preinitiation complexes or in living cells reveals physi

261 The "preloading" of T-RS into HIV-1 preinitiation complexes prevents the entry of active Tat

262 -depth characterization of RNA polymerase II preinitiation complexes remains an important and challen

263 oplasmic aggregates of stalled translational preinitiation complexes that accumulate during stress.

264 s granules (SGs) contain stalled translation preinitiation complexes that are assembled into discrete

265 /eIF2/GTP binds to 40S subunits yielding 43S preinitiation complexes that attach to the 5'-terminal r

266 n factors and Pol II to assemble on DNA into preinitiation complexes that can begin RNA synthesis upo

267 o the efficient recruitment of transcription preinitiation complexes to active promoters.

268 on codon selection and enables mammalian 43S preinitiation complexes to discriminate against AUG codo

269 with the presence of stalled 48S translation preinitiation complexes to persist throughout infection.

270 template assays in which activator-recruited preinitiation complexes were allowed to undergo one cycl

271 4 acidic activation domains in transcription preinitiation complexes were identified by site-specific

272 Initiation involves direct attachment of 43S preinitiation complexes within a short window at or imme

273 that nucleates the assembly of transcription preinitiation complexes, also independently interacts wi

274 We show that NUFIP is associated with preinitiation complexes, open transcription complexes, a

275 use the proteasome to target transcriptional preinitiation complexes, thus minimizing transcriptional

276 ted to stimulate attachment of 43S ribosomal preinitiation complexes, which then scan to the initiati

277 uitment and frequent reutilization of stable preinitiation complexes.

278 on by controlling the assembly of functional preinitiation complexes.

279 ted region of mRNA by scanning ribosomal 43S preinitiation complexes.

280 regulatory functions in protein translation preinitiation complexes.

281 eads to assembly of distinct transcriptional preinitiation complexes.

282 bly of archaeal and eukaryotic transcription preinitiation complexes.

283 vo and reduces 40S-binding of eIF3 to native preinitiation complexes.

284 ctors, and RPL41A mRNA to native 43S and 48S preinitiation complexes.

285 for U6 transcription and for assembly of U6 preinitiation complexes.

286 inhibition of the assembly of transcription preinitiation complexes.

287 e, both Ded1 and Gle1 affect the assembly of preinitiation complexes.

288 aggregates that contain stalled translation preinitiation complexes.

289 The enzyme adopts a closed "preinitiation" conformation similar to the one that was

290 which contain translationally silent mRNAs, preinitiation factors, and RNA-binding proteins.

291 IF3 subunits and several other translational preinitiation factors.

292 Preinitiation K was >5.0 mmol/L in 1.4% and Cr>2.5 mg/dL

293 sozyme does not inhibit the formation of the preinitiation open complex (open complex in the absence

294 In this study, we used preinitiation RNA replication complexes (PIRCs) to deter

295 ation with helper mRNAs allowed us to create preinitiation RNA replication complexes containing RNA t

296 of negative- and positive-strand RNAs within preinitiation RNA replication complexes, we found that m

297 l-free translation-replication reactions and preinitiation RNA replication complexes.

298 These structures refine the pathway from preinitiation through initiation to elongation for the R

299 y germ cells by blocking the transition from preinitiation to transcriptional elongation.

300 P) is a key component of the archaea ternary preinitiation transcription assembly.