ライフサイエンスコーパス: preinitiation complex

1 factor 3f, a repressive component in the 43S preinitiation complex.

2 binding protein and TFIID, components of the preinitiation complex.

3 hat modulate the binding of mRNA to the 43 S preinitiation complex.

4 e II holoenzyme and DNA, for assembly of the preinitiation complex.

5 ritical for assembly of TFIID and the pol II preinitiation complex.

6 directed reorganization in a transcriptional preinitiation complex.

7 n to direct appropriate assembly of the URA1 preinitiation complex.

8 ongation, or by the inability to establish a preinitiation complex.

9 osphorylation of ribosomal protein S6 on the preinitiation complex.

10 ion of the RNA polymerase II transcriptional preinitiation complex.

11 ormation of the RNA polymerase II-containing preinitiation complex.

12 D initiates formation of the transcriptional preinitiation complex.

13 AUG-dependent dissociation of eIF1 from the preinitiation complex.

14 g eIF5-promoted hydrolysis of GTP in the 40S preinitiation complex.

15 s crucial for the assembly of the eukaryotic preinitiation complex.

16 is is released upon AUG selection by the 40S preinitiation complex.

17 mitting transcriptional activators to form a preinitiation complex.

18 complex and RNA polymerase II to create the preinitiation complex.

19 embly of the RNA polymerase II transcription preinitiation complex.

20 itical for the production of functional 40 S preinitiation complex.

21 R079c-a, as a potential new component of the preinitiation complex.

22 or-P.5 was added after pol II entry into the preinitiation complex.

23 he assembly of a RNA polymerase III-specific preinitiation complex.

24 core promoter and initiates assembly of the preinitiation complex.

25 ning the integrity of the scanning ribosomal preinitiation complex.

26 -Rrn3 complex to the rDNA or stabilizing the preinitiation complex.

27 ing rapid promoter escape of Pol II from the preinitiation complex.

28 t to the closed conformation in the scanning preinitiation complex.

29 that required Beclin1, but not the autophagy preinitiation complex.

30 ion initiation begins with assembly of a 43S preinitiation complex.

31 own that it is a functional component of the preinitiation complex.

32 oprecipitated, and OGT is a component of the preinitiation complex.

33 e context of a fully assembled transcription preinitiation complex.

34 polymerase II occurs during assembly of the preinitiation complex.

35 preliminary picture of the RNA polymerase II preinitiation complex.

36 of the mRNA-unwinding machinery to the 43 S preinitiation complex.

37 en complex results in destabilization of the preinitiation complex.

38 that it involves alterations within the 48S preinitiation complex.

39 es and AUG act coordinately to stabilize the preinitiation complex.

40 uitment and frequent reutilization of stable preinitiation complexes.

41 on by controlling the assembly of functional preinitiation complexes.

42 ted region of mRNA by scanning ribosomal 43S preinitiation complexes.

43 regulatory functions in protein translation preinitiation complexes.

44 eads to assembly of distinct transcriptional preinitiation complexes.

45 bly of archaeal and eukaryotic transcription preinitiation complexes.

46 vo and reduces 40S-binding of eIF3 to native preinitiation complexes.

47 ctors, and RPL41A mRNA to native 43S and 48S preinitiation complexes.

48 for U6 transcription and for assembly of U6 preinitiation complexes.

49 inhibition of the assembly of transcription preinitiation complexes.

50 e, both Ded1 and Gle1 affect the assembly of preinitiation complexes.

51 aggregates that contain stalled translation preinitiation complexes.

52 tion factors eIF1, eIF1A and eIF3 in the 40S preinitiation complex (40S.eIF1.eIF1A.eIF3.Met-tRNA(i).e

53 lity, the hydrolysis of GTP bound to the 40S preinitiation complex (40S.Met-tRNA(i).eIF2.GTP), promot

54 g eukaryotic translation initiation, the 43S preinitiation complex (43S PIC), consisting of the 40S r

55 s that a structural rearrangement in the 43S preinitiation complex activates it to become fully compe

56 Phosphorylation of components of the preinitiation complex activates replication and prevents

57 Thus, the eIF3 preinitiation complex acts as a scaffold to coordinate a

58 c1Delta mutation affects the assembly of the preinitiation complex after osmotic shock, it does not a

59 kinetic dissection of the transition from a preinitiation complex after start codon recognition to t

60 that nucleates the assembly of transcription preinitiation complexes, also independently interacts wi

61 s that nucleate the assembly of a functional preinitiation complex and integrate stimulatory and repr

62 To study TBP functions in preinitiation complex and other complexes, we generated

63 gene-specific transcription factors with the preinitiation complex and RNA polymerase II.

64 f mRNA and stimulates recruitment of the 43S preinitiation complex and subsequent scanning to the ini

65 anding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and

66 4me3 that leads to assembly of transcription preinitiation complex and transcriptional activation.

67 , for Mediator-dependent assembly of a basal preinitiation complex and, more important, identify a st

68 P1-NTD is required for efficient assembly of preinitiation complexes and also regulates the selection

69 nd Brf1 is detected at 0 degrees C in closed preinitiation complexes and at 30 degrees C in complexes

70 3j/HCR1 remains associated with the scanning preinitiation complexes and does not dissociate from the

71 -a is a bona fide component of polymerase II preinitiation complexes and investigate its role in tran

72 to be nonessential for eIF3 function to form preinitiation complexes and it may function as a regulat

73 ble binding of at least some mRNAs to native preinitiation complexes and that eIF4G has a rate-limiti

74 a model whereby BRCA1 stabilizes productive preinitiation complexes and thus stimulates transcriptio

75 apoptosis, the assembly of RNA polymerase II preinitiation complexes and WNT signalling.

76 is a central component of the transcription preinitiation complex, and its occupancy at a promoter i

77 In this study, the components of the de novo preinitiation complex are defined as ATP, a high concent

78 core promoter of RNR3 is sufficient to drive preinitiation complex assembly and activate transcriptio

79 0S binding of eIF3 and is needed for optimal preinitiation complex assembly and AUG recognition in vi

80 EAT domain is a critical nucleation core for preinitiation complex assembly and function.

81 ontrol global gene expression in eukaryotes: preinitiation complex assembly and polymerase pausing.

82 re, a TFIIB-related protein is implicated in preinitiation complex assembly and postpolymerase recrui

83 ations of these results for the mechanism of preinitiation complex assembly and promoter melting.

84 y by the activator Gcn4p but is dependent on preinitiation complex assembly and Ser5 carboxy-terminal

85 oter chromatin remodeling from transcription preinitiation complex assembly and suggest the existence

86 sis coupled with native gel electrophoresis, preinitiation complex assembly assays, and translation i

87 8p and H3-K4 methylation are dispensable for preinitiation complex assembly at the core promoters of

88 ability of chromatin-bound Gcn4 to stimulate preinitiation complex assembly at the promoter.

89 inct mechanisms for ribosome recruitment and preinitiation complex assembly between the two processes

90 can be used to learn how different modes of preinitiation complex assembly impact transcriptional ac

91 IIB derivative is able to support high order preinitiation complex assembly in the absence of an acti

92 However, it is unknown how preinitiation complex assembly is coordinated with chrom

93 required for activators to stimulate Pol II preinitiation complex assembly on an associated promoter

94 with the MED23 Mediator subunit, stimulating preinitiation complex assembly on early viral promoters

95 activation domains with mediator stimulates preinitiation complex assembly on promoter DNA.

96 om the TAF1-TFIID complex upon completion of preinitiation complex assembly, allowing transcription t

97 ated GR attenuated ISGF3 promoter occupancy, preinitiation complex assembly, and ISG expression.

98 t Mdm30p is dispensable for formation of the preinitiation complex assembly, association of elongatin

99 inhibiting an essential function of TFIIF in preinitiation complex assembly, but also that Mediator c

100 rent steps in transcription before and after preinitiation complex assembly.

101 sferase-independent function at the level of preinitiation complex assembly.

102 ion elongation factor TFIIS functions during preinitiation complex assembly.

103 h, which allows TFIID binding and subsequent preinitiation complex assembly.

104 te Pol II initiation at a step subsequent to preinitiation complex assembly.

105 MFC), an important intermediate for the 43 S preinitiation complex assembly.

106 T1/Cdk9 inhibits PGC-1 promoter activity and preinitiation complex assembly.

107 ding to core promoter elements and directing preinitiation complex assembly.

108 lucosaminidase) blocked transcription during preinitiation complex assembly.

109 g transcriptional activators and facilitates preinitiation-complex assembly and transcription.

110 Consistent with this finding, Mediator and preinitiation complex association with SAGA-dependent pr

111 e present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 A resolution with initiator

112 on network is essential for formation of the preinitiation complex at the core promoter to initiate t

113 ation without disrupting the assembly of the preinitiation complex at the cyp1a1 promoter.

114 olymerase II, nucleating the assembly of the preinitiation complex at the transcription start site.

115 hich is thought to stabilize properly formed preinitiation complexes at the correct start codon.

116 CDK8 module are specifically recruited into preinitiation complexes at the TR target gene type I dei

117 like unexpressed genes without transcription-preinitiation complexes at their promoters, expressed ge

118 ation of most eukaryotic mRNAs occurs when a preinitiation complex binds to the 5' cap, scans the mRN

119 s bind immediately downstream of the Pol III preinitiation complex but are not required for Pol III r

120 ire the preloading of RNA polymerase II or a preinitiation complex but instead depends upon the Gcn5

121 e the assembly of RNA polymerase II (Pol II) preinitiation complexes, but there have been few tests o

122 rug influences the assembly and stability of preinitiation complexes by targeting the interaction bet

123 This suggests that functional preinitiation complexes can contain Mot1 instead of TFII

124 of eIF1 was identified, interfaces to other preinitiation complex components and their relevance to

125 caffold for the dynamic associations of many preinitiation complex components, including the growth-r

126 et out to fine-map a small viral replication preinitiation complex composed of two protein dimers bou

127 First, 43S preinitiation complexes comprising 40S ribosomal subunit

128 e of mediating assembly of the transcription preinitiation complex containing RNA polymerase II.

129 iation in Archaea requires the assembly of a preinitiation complex containing the TATA- box binding p

130 nslation initiation factor (eIF)3 enable 43S preinitiation complexes containing eIF3 and eIF2-GTP-Met

131 binding to Adr1 at promoters that contain a preinitiation complex, demonstrating that Bmh-mediated i

132 nt in pol III recruitment, but the resulting preinitiation complex does not open the promoter.

133 gly, the initiation codon recognition by 43S preinitiation complex during EXT2 translation is suppres

134 TFEalpha/beta stabilises the preinitiation complex, enhances DNA melting, and stimula

135 omoters, expressed genes or genes containing preinitiation complexes exhibit characteristic nucleosom

136 of coregulators, leading to the formation of preinitiation complex followed by elongation.

137 initiation involves the assembly of the 48S preinitiation complex, followed by joining of the 60S ri

138 able to scan template DNA downstream of the preinitiation complex for acceptable start sites.

139 IID (TFIID), which nucleates assembly of the preinitiation complex for transcription by RNA polymeras

140 The pathways by which preinitiation complexes form, and how this impacts trans

141 reagents, their effect on transcription and preinitiation complex formation and discuss their potent

142 ter escape are linked to stronger effects on preinitiation complex formation and transcription, sugge

143 on it alters chromatin structure to increase preinitiation complex formation and transcription.

144 omote dephosphorylation of Ser5 to stimulate preinitiation complex formation and, later, to inhibit e

145 ts with promoter conformation and downstream preinitiation complex formation and/or function.

146 associated factors (TAFs), is essential for preinitiation complex formation at ribosomal RNA gene pr

147 rved cis elements, the TFIID complex directs preinitiation complex formation at specific sites in cor

148 e context-dependent Saccharomyces cerevisiae preinitiation complex formation at the HIS4 promoter rec

149 egative regulation of transcription, correct preinitiation complex formation in basal and activated t

150 Furthermore, Tup1 repressed RNR3 and blocked preinitiation complex formation in the Deltaisw2 mutant,

151 ubunit, in Pol I recruitment and, therefore, preinitiation complex formation in vivo.

152 unctions both in chromatin remodeling and in preinitiation complex formation or function (transcripti

153 opose a model of stochastic enhanceosome and preinitiation complex formation that incorporates transc

154 anning sequences downstream from the site of preinitiation complex formation, a process that involves

155 ARG1 is independent of the TATA element and preinitiation complex formation, whereas efficient recru

156 osmoresponse gene transcription by enhancing preinitiation complex formation.

157 histone acetylation) at a step subsequent to preinitiation complex formation.

158 alterations and subsequent RNA polymerase II preinitiation complex formation.

159 ily blocks steps downstream of transcription preinitiation complex formation.

160 of TATA box genes appear to be controlled at preinitiation complex formation.

161 arts of the IRES RNA change structure as the preinitiation complex forms.

162 scription factor IIB and dissociation of the preinitiation complex from the major promoter.

163 nesis requires SWI/SNF enzymes to facilitate preinitiation complex function.

164 SNF affects recruitment of components of the preinitiation complex in a promoter-specific manner to m

165 important role for RLI1 in assembly of 43 S preinitiation complexes in vivo.

166 neral transcription factors that make up the preinitiation complex, including Pol II, but there was n

167 air GCN4 translational control by disrupting preinitiation complex interactions.

168 onal changes that mark the transition of 30S preinitiation complex into elongation competent 70S comp

169 n by BRCA1 is that the ubiquitination of the preinitiation complex is not targeting proteins for degr

170 se that the overall structure of the RNAP II preinitiation complex is similar in all eukaryotes and i

171 tial component of the RNA polymerase (pol) I preinitiation complex, is unclear.

172 TOR), and appears to represent an incomplete preinitiation complex lacking several subunits.

173 ng the formation of stable RNA polymerase II preinitiation complexes leading to transcription initiat

174 lating the assembly of the 48S translational preinitiation complex mediated by the p27 IRES element.

175 d the AdMLP suggests that Rep78/68 alter the preinitiation complex of RNA polymerase II transcription

176 ation of mda-7/IL-24 mRNA, activation of the preinitiation complex of the translational machinery in

177 of rrnB P1 and Fis-mediated stabilization of preinitiation complexes of the promoter.

178 he other factors necessary for assembly of a preinitiation complex on naked DNA.

179 erentially affects the assembly of ribosomal preinitiation complexes on different cellular and viral

180 PSEA, DmSNAPc establishes RNA polymerase II preinitiation complexes on U1 to U5 promoters but RNA po

181 on U1 to U5 promoters but RNA polymerase III preinitiation complexes on U6 promoters.

182 We show that NUFIP is associated with preinitiation complexes, open transcription complexes, a

183 cription by interfering with assembly of the preinitiation complex or by blocking transcription initi

184 regulate later stages in the assembly of the preinitiation complex or facilitate multiple rounds of p

185 However, RNA pol III bound to preinitiation complexes or in elongation complexes is pr

186 se TBP derivatives in isolated transcription preinitiation complexes or in living cells reveals physi

187 roteins, but not the conventional autophagic preinitiation complex, or adaptor protein-3 (AP-3).

188 ge number of promoters assemble into partial preinitiation complexes (partial PICs), containing TFIIA

189 Dissociation of eIF1 from the preinitiation complex (PIC) allows release of phosphate

190 omain (eIF5-CTD) directly links eIF4G to the preinitiation complex (PIC) and enhances mRNA binding.

191 of melted DNA separately associated with the preinitiation complex (PIC) and the adjacent paused comp

192 anslation initiation factor eIF1A stimulates preinitiation complex (PIC) assembly and scanning, but t

193 ription factor TFIIB plays a central role in preinitiation complex (PIC) assembly and the recruitment

194 SIR-mediated silencing is permissive to both preinitiation complex (PIC) assembly and transcription i

195 e GAL1 UAS, and facilitates formation of the preinitiation complex (PIC) assembly at the GAL1 promote

196 he steps leading to chromatin remodeling and preinitiation complex (PIC) assembly differ significantl

197 A "step-wise" preinitiation complex (PIC) assembly model has been sugg

198 specific activator NF-E2 to the promoter and preinitiation complex (PIC) assembly occur only after di

199 (PRC1) inhibits activated RNA polymerase II preinitiation complex (PIC) assembly using immobilized H

200 FIIF are both required for RNA polymerase II preinitiation complex (PIC) assembly, but their roles at

201 he structure of transcription factors (TFs), Preinitiation Complex (PIC) assembly, RNA polymerase II

202 ween VP16-mediated chromatin acetylation and preinitiation complex (PIC) assembly.

203 nscription factor TFIID, which might promote preinitiation complex (PIC) assembly.

204 events PESE-dependent Pol II recruitment and preinitiation complex (PIC) assembly.

205 anscription initiation and play key roles in preinitiation complex (PIC) assembly.

206 ies of highly regulated steps: assembly of a preinitiation complex (PIC) at the promoter nucleated by

207 s of highly regulated steps: assembly of the preinitiation complex (PIC) at the promoter, initiation,

208 vating protein complex, form a transcription preinitiation complex (PIC) at the spliced leader (SL) R

209 olving mRNA secondary structures that impede preinitiation complex (PIC) attachment to mRNA or scanni

210 The eukaryotic 43S preinitiation complex (PIC) bearing Met-tRNAi(Met) in a

211 defective for interaction with polymerase II preinitiation complex (PIC) components and other regulat

212 The resulting complete pol II transcription preinitiation complex (PIC) contained equimolar amounts

213 steps in transcription initiation including preinitiation complex (PIC) formation and start site sel

214 ans is regulated in several steps, including preinitiation complex (PIC) formation, initiation, Pol I

215 III dependent transcription before and after preinitiation complex (PIC) formation.

216 s p53-dependent transcription by stimulating preinitiation complex (PIC) formation; (2) H3K4me3, thro

217 scription commences with the assembly of the Preinitiation Complex (PIC) from a plethora of proteins

218 work implicated eIF5 in rearrangement of the preinitiation complex (PIC) from an open, scanning confo

219 ensus motif for the positioning of the human preinitiation complex (PIC) has been identified.

220 complexes remove the -1 nucleosome after the preinitiation complex (PIC) has partially assembled, but

221 ferences of the key components of eukaryotic preinitiation complex (PIC) have been recently measured

222 promoting ATP-dependent dissociation of the preinitiation complex (PIC) into the Scaffold complex.

223 don in an mRNA by the eukaryotic translation preinitiation complex (PIC) is essential for proper gene

224 The translation preinitiation complex (PIC) is thought to assume an open

225 1), eIF1A, eIF3, and eIF5, and the resulting preinitiation complex (PIC) joins the 5' end of mRNA pre

226 The formation of a stable 43 S preinitiation complex (PIC) must occur to enable success

227 that a reduced affinity of eIF3j for the 43S preinitiation complex (PIC) occurs on eIF4F-dependent mR

228 The assembly of the preinitiation complex (PIC) occurs upstream of the +1 nu

229 ns with the assembly of an RNA polymerase II preinitiation complex (PIC) on the promoter.

230 oactivators are required for assembly of the preinitiation complex (PIC) or for subsequent steps in t

231 ires the assembly at the promoter of a large preinitiation complex (PIC) that includes RNA polymerase

232 inately recruit RNA polymerase II and form a preinitiation complex (PIC) to activate MyoD transcripti

233 as been implicated in attachment of the 43 S preinitiation complex (PIC) to mRNAs and scanning to the

234 ve reconstituted mRNA recruitment to the 43S preinitiation complex (PIC) using purified S. cerevisiae

235 e of a 33-protein, 1.5-MDa RNA polymerase II preinitiation complex (PIC) was determined by cryo-EM an

236 onents of a large RNA polymerase II (Pol II) preinitiation complex (PIC) were distinguished from a ba

237 nucleates the assembly of the transcription preinitiation complex (PIC), and although TBP can bind p

238 x and its role, along with components of the preinitiation complex (PIC), in histone eviction at indu

239 s H2A and H2B, Nap1p, and a component of the preinitiation complex (PIC), TBP.

240 formation and function of the promoter-bound preinitiation complex (PIC), which consists of RNA polym

241 ted steps in the assembly of a transcription preinitiation complex (PIC).

242 with RNA polymerase (Pol) II as part of the preinitiation complex (PIC).

243 the RNA polymerase II (Pol II) transcription preinitiation complex (PIC).

244 on domain on Pol II within the transcription preinitiation complex (PIC).

245 ment for TBP recruitment and assembly of the preinitiation complex (PIC).

246 inhibits the assembly and the function of a preinitiation complex (PIC).

247 ption factors and promoter DNA in a 'closed' preinitiation complex (PIC); unwinding of about 15 base

248 if32 CTD that impair mRNA recruitment by 43S preinitiation complexes (PICs) and confer phenotypes ind

249 o determine how Mot1 affects the assembly of preinitiation complexes (PICs) at Mot1-controlled promot

250 manner suggesting incomplete assembly of 48S preinitiation complexes (PICs) at upstream AUG codons in

251 bly of RNA polymerase (Pol) II transcription preinitiation complexes (PICs) have been well establishe

252 eomic analysis of RNA polymerase II (RNApII) preinitiation complexes (PICs) identified Sub1 and the r

253 However, pol II preinitiation complexes (PICs) lose the ability to synth

254 The accumulation of stalled translation preinitiation complexes (PICs) mediates the condensation

255 (MuDPIT) and immunoblot analyses of purified preinitiation complexes (PICs) revealed the recruitment

256 n by facilitating the recruitment of RNAP II preinitiation complexes (PICs) to the promoter regions o

257 face that is required for rapid formation of preinitiation complexes (PICs) was identified on the N-t

258 apid, TATA box-dependent assembly of RNAP II preinitiation complexes (PICs), but permits few rounds o

259 rprisingly, when Gdown1 is added to complete preinitiation complexes (PICs), it does not inhibit init

260 isolated that affect either the assembly of preinitiation complexes (PICs), scanning for AUG, or bot

261 ociation and P(i) release from reconstituted preinitiation complexes (PICs), whereas a hyperaccuracy

262 of chromatin and the assembly of functional preinitiation complexes (PICs), which contain the genera

263 ngation, but such information is lacking for preinitiation complexes (PICs).

264 ometric screen of RNA polymerase II (Pol II) preinitiation complexes (PICs).

265 complex [TC] with eIF2-GTP) to reconstituted preinitiation complexes (PICs).

266 tiation of DNA synthesis from each origin, a preinitiation complex (pre-IC) containing Cdc45 and othe

267 and a core component of the DNA replication preinitiation complex (pre-IC), and that the TICRR-TopBP

268 ated stabilization of RNA polymerase-rrnB P1 preinitiation complexes, presumably at the open complex

269 BRCA1 ubiquitinates the transcriptional preinitiation complex, preventing stable association of

270 The "preloading" of T-RS into HIV-1 preinitiation complexes prevents the entry of active Tat

271 he involvement of MED25 for fully functional preinitiation complex recruitment and transcriptional ou

272 target genes was manifested at the level of preinitiation complex recruitment.

273 -depth characterization of RNA polymerase II preinitiation complexes remains an important and challen

274 scription by blocking the recruitment of the preinitiation complex (RNA polymerase II and TFIIB) to t

275 oter recruitment of poised RNA polymerase II preinitiation complex (RNAPII PIC), which enhances futur

276 nd eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recog

277 rred, and the activators recruited a partial preinitiation complex that included RNA polymerase II.

278 slation initiation in eukaryotes occurs in a preinitiation complex that includes small ribosomal subu

279 dues of eIF1 disrupts a critical link to the preinitiation complex that suppresses eIF1 release befor

280 information for the papillomavirus E1E2-ori preinitiation complex that would otherwise have been har

281 oplasmic aggregates of stalled translational preinitiation complexes that accumulate during stress.

282 s granules (SGs) contain stalled translation preinitiation complexes that are assembled into discrete

283 /eIF2/GTP binds to 40S subunits yielding 43S preinitiation complexes that attach to the 5'-terminal r

284 n factors and Pol II to assemble on DNA into preinitiation complexes that can begin RNA synthesis upo

285 s impair the integrity of scanning-competent preinitiation complex, thereby altering the 40 S subunit

286 e the interaction with the components of the preinitiation complex, thereby inhibiting its function a

287 use the proteasome to target transcriptional preinitiation complexes, thus minimizing transcriptional

288 .eIF2.GTP) and the subsequent binding of the preinitiation complex to eIF4F bound at the 5'-cap struc

289 ription by inhibiting the recruitment of the preinitiation complex to the c-jun promoter.

290 o the efficient recruitment of transcription preinitiation complexes to active promoters.

291 on codon selection and enables mammalian 43S preinitiation complexes to discriminate against AUG codo

292 with the presence of stalled 48S translation preinitiation complexes to persist throughout infection.

293 method to analyze a yeast RNA polymerase II preinitiation complex, we identified a new 8-kDa protein

294 template assays in which activator-recruited preinitiation complexes were allowed to undergo one cycl

295 4 acidic activation domains in transcription preinitiation complexes were identified by site-specific

296 of EIF1AX, a component of the translational preinitiation complex, were markedly enriched in PDTCs a

297 lymerase II (Pol II) with the promoter-bound preinitiation complex, whereas Brf1 and Brf2 are involve

298 ly bind to the 40S subunit, yielding the 43S preinitiation complex, which is ready to attach to messe

299 ted to stimulate attachment of 43S ribosomal preinitiation complexes, which then scan to the initiati

300 Initiation involves direct attachment of 43S preinitiation complexes within a short window at or imme