ライフサイエンスコーパス: preintegration

1 odel in which APOBEC3B or APOBEC3F provide a preintegration barrier to L1 retrotransposition.

2 Here, we show that the preintegration complex (i.e., the excised transposon) is

3 ne breakdown during cell division, the HIV-1 preintegration complex (PIC) enters the nucleus by trave

4 uired for nuclear translocation of the viral preintegration complex (PIC) in quiescent cells.

5 human immunodeficiency virus type 1 (HIV-1) preintegration complex (PIC) is essential for the produc

6 eficiency virus type 1 (HIV-1) infection and preintegration complex (PIC) nuclear import.

7 reverse transcription is a component of the preintegration complex (PIC) that also contains the vira

8 ion, the retroviral genome is contained in a preintegration complex (PIC) that mediates its integrati

9 LV) p12, encoded within Gag, binds the viral preintegration complex (PIC) to the mitotic chromatin.

10 gate the role of Vpr in docking of the HIV-1 preintegration complex (PIC) to the nuclear pore complex

11 Retroviral integration is mediated by a preintegration complex (PIC) which contains the viral DN

12 rse transcription complex and later with the preintegration complex (PIC), allowing it to reach and e

13 kemia virus (MLV) Gag is associated with the preintegration complex (PIC), and mutants of p12 (PM14)

14 by a large nucleoprotein complex, termed the preintegration complex (PIC).

15 g in an inability to form a functional viral preintegration complex (PIC).

16 1 has been implicated in import of the viral preintegration complex across the nuclear pore complex (

17 ulted in a decrease in nuclear import of the preintegration complex and attenuated replication in mac

18 would facilitate nuclear import of the viral preintegration complex and transduction of quiescent cel

19 nscription and nuclear transfer of the viral preintegration complex are observed.

20 Nuclear targeting of the preintegration complex by an IN NLS may prove to be a ge

21 itate the study of the roles of IN and other preintegration complex components in preintegration phas

22 r DCTN2/p50/dynamitin interacts with the MLV preintegration complex early in infection, suggesting a

23 -1) replication is the movement of the viral preintegration complex from the cytoplasm into the nucle

24 ocess by which the avian sarcoma virus (ASV) preintegration complex gains access to target chromatin

25 mbrane, envelope incorporation into virions, preintegration complex import into the nucleus, and nucl

26 port cargoes, the driving force behind HIV-1 preintegration complex import is likely a gradient of th

27 rticipates in nuclear targeting of the viral preintegration complex in nondividing cells and induces

28 BAF also facilitates retroviral preintegration complex insertion into target DNA in vitr

29 rmitting the translocation of the retroviral preintegration complex into the nucleus and enabling int

30 ription, DNA synthesis, and translocation of preintegration complex into the nucleus in cord and adul

31 The transport of the preintegration complex into the nucleus is cell cycle-in

32 A model in which TRN-SR2 imports the viral preintegration complex into the nucleus is supported by

33 ls depends critically on import of the viral preintegration complex into the nucleus.

34 ng the role of integrase in transport of the preintegration complex into the nucleus.

35 cells such as macrophages because the viral preintegration complex is able to actively traverse the

36 and GLFG Nups and that nuclear entry of the preintegration complex is further promoted by nuclear lo

37 tions between a gypsy provirus and the gypsy preintegration complex may also participate in the proce

38 NA flap does not play a major role in either preintegration complex nuclear import or HIV-1 replicati

39 We found that the preintegration complex of murine leukemia virus (MLV) in

40 ns in mitotic nuclear reassembly, retroviral preintegration complex stability, and transcriptional re

41 and LEDGF/p75 in the targeting of the viral preintegration complex to gene-dense regions of chromati

42 ociate from the viral membrane to direct the preintegration complex to the host-cell nucleus.

43 gnal that promotes localization of the viral preintegration complex to the nucleus of non-dividing ce

44 hitecture remain intact upon delivery of the preintegration complex to the nucleus.

45 transcription but prior to migration of the preintegration complex to the nucleus.

46 ey molecular interactions that specify HIV-1 preintegration complex trafficking to active chromatin.

47 Cell fractionation showed that the viral preintegration complex was present in a form that could

48 chromosome, can suffice to connect the HIV-1 preintegration complex with the cell nuclear import mach

49 grase (IN) is the catalytic component of the preintegration complex, a large nucleoprotein assembly c

50 etroviral DNA integration is mediated by the preintegration complex, a large nucleoprotein complex de

51 RK1 and -2 appear to phosphorylate the HIV-1 preintegration complex, a step necessary for nuclear tra

52 pecific antigen (Gag) is associated with the preintegration complex, and mutants of p12 (PM14) show d

53 alization, participation in transport of the preintegration complex, cation channel activity, oligome

54 Vpr regulates nuclear transport of the viral preintegration complex, G(2) cell cycle arrest, and tran

55 tations demonstrate that in vivo, within the preintegration complex, IN performs a central role in co

56 Vpr mediates nuclear import of the preintegration complex, induces host cell apoptosis, and

57 lex in retrovirus-infected cells, termed the preintegration complex, is responsible for the concerted

58 reverse transcription, nuclear import of the preintegration complex, or viral DNA integration, sugges

59 ll cycle arrest, apoptosis, translocation of preintegration complex, potentiation of glucocorticoid a

60 v1 gene product and a component of the viral preintegration complex, the capsid protein CA.

61 rocess of nuclear translocation of the viral preintegration complex, thus facilitating HIV-1 replicat

62 rase-binding domain interacts with the viral preintegration complex, whereas the N-terminal PWWP doma

63 irements for retrograde transport of the MLV preintegration complex.

64 ecessary for efficient nuclear import of the preintegration complex.

65 estigate the nuclear import of the HIV-2/SIV preintegration complex.

66 of Vpx in nuclear translocation of the viral preintegration complex.

67 intasome to distinguish it from the greater preintegration complex.

68 f the karyophilic MA into the viral core and preintegration complex.

69 involved in the nuclear translocation of the preintegration complex.

70 infected cells as part of the nucleoprotein/preintegration complex.

71 y gain access to viral proteins of the HIV-1 preintegration complex.

72 n gain access to viral proteins of the HIV-1 preintegration complex.

73 y, and shares properties associated with the preintegration complex.

74 ient to account for its association with the preintegration complex.

75 ransient intermediate within the cytoplasmic preintegration complex.

76 process of nuclear translocation of the HIV preintegration complex.

77 ing infectivity for nuclear targeting of the preintegration complex.

78 plays a role in nuclear import of the viral preintegration complex.

79 Retrovirus preintegration complexes (PIC) in virus-infected cells c

80 Retrovirus preintegration complexes (PIC) purified from virus-infec

81 N) into the host genome occurs via assembled preintegration complexes (PIC).

82 ecular-weight nucleoprotein complexes termed preintegration complexes (PIC).

83 These preintegration complexes (PICs) can direct integration o

84 ion, it is possible to lyse cells and obtain preintegration complexes (PICs) capable of integrating t

85 etroviral integration in vivo is mediated by preintegration complexes (PICs) derived from infectious

86 r of viral factors that are present in HIV-1 preintegration complexes (PICs) have been assigned funct

87 proteins and examined their association with preintegration complexes (PICs) in infected cells.

88 own to aid the nuclear localization of viral preintegration complexes (PICs) in infected cells.

89 t protein is important for function of HIV-1 preintegration complexes (PICs) in vitro.

90 arly reduced the efficiency with which HIV-1 preintegration complexes (PICs) integrated into a target

91 are actively dividing, and nuclear import of preintegration complexes (PICs) is not required for infe

92 A nonspecifically and is a host component of preintegration complexes (PICs) isolated from cells infe

93 rted that HMG I(Y) cofractionates with HIV-1 preintegration complexes (PICs) isolated from freshly in

94 vitro on purified integrase and on subviral preintegration complexes (PICs) isolated from lymphoid c

95 Preintegration complexes (PICs) mediate retroviral integ

96 ave established an assay for the function of preintegration complexes (PICs) of human immunodeficienc

97 In vitro integration assays with preintegration complexes (PICs) showed that endogenous L

98 Preintegration complexes (PICs) stripped of BAF lose the

99 and U5 ends of viral cDNA or by using viral preintegration complexes (PICs) that form during virus i

100 t factors to facilitate the passage of their preintegration complexes (PICs) through nuclear pore com

101 growth factor (LEDGF/p75) tethers lentiviral preintegration complexes (PICs) to chromatin and is esse

102 Retroviral integration is mediated by viral preintegration complexes (PICs), and human immunodeficie

103 human immunodeficiency virus type 1 (HIV-1) preintegration complexes (PICs), the large nucleoprotein

104 integrases (INs) function in the context of preintegration complexes (PICs).

105 tion and subcellular trafficking of subviral preintegration complexes are reported to vary among the

106 Once inside the nucleus, lentiviral preintegration complexes are thought to attach to the ch

107 ssociation of BAF from retroviral DNA within preintegration complexes as monitored by functional assa

108 inhibits nuclear targeting of HIV-1-derived preintegration complexes by inactivating the nuclear loc

109 ested HeLa cells, indicating that the mutant preintegration complexes can enter the nuclei of both di

110 Preintegration complexes extracted from knockout cells m

111 Further, the preintegration complexes extracted from the cytoplasm of

112 of sufficient quantities of the cytoplasmic preintegration complexes for biochemical and biophysical

113 , which is equivalent to isolated retrovirus preintegration complexes for full-site integration activ

114 We prepared preintegration complexes from cells infected with these

115 py allow three-dimensional analysis of HIV-1 preintegration complexes in the nuclei of infected cells

116 ed with MLV and HIV-1 in vivo and with their preintegration complexes in vitro.

117 The transport of virus preintegration complexes into the nucleus in primary mac

118 that the impairment of nuclear transport of preintegration complexes is responsible for the restrict

119 us to analyze the structure and function of preintegration complexes isolated from cells infected wi

120 Previous transposon-mediated footprinting of preintegration complexes isolated from infected cells re

121 outhern blotting of S1 nuclease-digested FIV preintegration complexes isolated from infected cells, w

122 These ruptures are sufficient to enable the preintegration complexes of invading virions to enter th

123 s with the ability to mature into functional preintegration complexes that can proceed to provirus es

124 g that binds HIV-1 capsid and connects HIV-1 preintegration complexes to intranuclear trafficking pat

125 Preintegration complexes were partially purified from ce

126 peat, participation in the nuclear import of preintegration complexes, induction of G2 arrest, and in

127 reverse transcription and nuclear import of preintegration complexes, we found that memory, but not

128 icate that HMG I(Y) is associated with MoMLV preintegration complexes.

129 human immunodeficiency virus type I (HIV-I) preintegration complexes.

130 diated blockade of productive infection from preintegration complexes.

131 ive intasome structure detected in wild-type preintegration complexes.

132 rganization of Moloney murine leukemia virus preintegration complexes.

133 tegrase and the viral DNA ends in functional preintegration complexes.

134 for such inhibitors in assays using isolated preintegration complexes.

135 ed in the nuclear localization of retroviral preintegration complexes.

136 host was specifically restricted by Fv-1 to preintegration events.

137 is combination vector, which displays strong preintegration inhibition of HIV-1 infection in vitro, c

138 donor and a circular acceptor containing the preintegration locus AAVS1.

139 In the "preintegration" phase, transforming plasmid molecules (e

140 d other preintegration complex components in preintegration phases of infection by (i) providing an a

141 the ability of type I IFN to inhibit early, preintegration phases of the HIV-1 replication cycle in

142 which both the full-length provirus and the preintegration site alleles are shown to be present in t

143 An unoccupied preintegration site also was present at this locus in tw

144 Humans contain an intact preintegration site at this locus.

145 Intact preintegration sites for each of these eight proviruses

146 of other mobile elements inserting near the preintegration sites of L1Hs preTa elements were observe

147 cifically neutralizes the RT activity in the preintegration stage and affects the reverse transcripti

148 rus type 1 (HIV-1) infection at a postentry, preintegration stage in the viral life cycle, by recogni

149 Our results demonstrate that the preintegration state is labile and decays rapidly (half-

150 st latently infected cells are in the labile preintegration state of latency.

151 linear viral genome and the stability of the preintegration state, we employed a recombinant HIV-1 vi

152 ontroversial whether HIV-1 is stable in this preintegration state.

153 easure the rate at which HIV-1 decays in the preintegration state.

154 gmentation by MRP-1 occur predominantly at a preintegration step but act through different mechanisms

155 Vif are severely restricted at a postentry, preintegration step of infection, it is presumed that su

156 nd I-XW-053, crippled the virus at an early, preintegration step.

157 , targeting incoming capsids at a postentry, preintegration step.

158 domain (NTD) of HIV-1 CA and disrupt early, preintegration steps of the HIV-1 replication cycle.

159 m of A3G, HIV-1 replication is restricted at preintegration steps, before accumulation of Vif.

160 ted species, we accurately reconstructed the preintegration target site sequence and deduced nucleoti

161 s that affected reverse transcription and/or preintegration trafficking than the catalytic activity o

162 Since preintegration transcription also generates Rev, to elim

163 ependent indicator cell, Rev-CEM, to measure preintegration transcription based on the amount of Rev

164 This preintegration transcription in quiescent cells leads to

165 Preintegration transcription is an early process in huma

166 rt, using Rev-CEM cells, we demonstrate that preintegration transcription occurs on a much larger sca