ライフサイエンスコーパス: prenyltransferase

1 esolution picture of a non-mammalian protein prenyltransferase.

2 to a conserved P-loop motif in this class of prenyltransferases.

3 FTase and is conserved in all known protein prenyltransferases.

4 c substrate isopentenyl diphosphate in other prenyltransferases.

5 eral common features with flavonoid-specific prenyltransferases.

6 the structures accessed by bacterial indole prenyltransferases.

7 ither cis-prenyltransferases (CPTs) or trans-prenyltransferases.

8 logies with known flavonoid and isoflavonoid prenyltransferases.

9 l and functional similarity to other protein prenyltransferases.

10 enzymes such as the isoprenoid synthases and prenyltransferases.

11 We also identified a conventional cis-prenyltransferase 3 (CPT3), exclusively expressed in lat

12 ed in up to 30% of all human cancers, making prenyltransferases a viable target for chemotherapeutic

13 nylated stilbenoids suggested that these two prenyltransferase activities represent the first committ

14 t time, a membrane-bound stilbenoid-specific prenyltransferase activity from the microsomal fraction

15 K-ras knockdown by siRNA or inhibition of prenyltransferase activity resulted in radiation sensiti

16 nonradioactive assay can be used to measure prenyltransferase activity under either single or multip

17 ssary (but not sufficient) for activation of prenyltransferase activity.

18 each cDNA individually yielded no detectable prenyltransferase activity; however, coexpression of the

19 na benthamiana We observed that one of these prenyltransferases, AhR4DT-1, catalyzes a key reaction i

20 prenyl diphosphate synthase, and an aromatic prenyltransferase allow formation of a highly unusual ar

21 The cyanobacterial prenyltransferase AmbP3 catalyzes the reverse prenylatio

22 er consisting of the synthase AN1242 and the prenyltransferase AN11080, as well as identification of

23 We systematically catalogued 32 unique prenyltransferases and 167 unique substrates to create p

24 les, other examples of which include protein prenyltransferases and cobalamin-independent methionine

25 logues for the study of a variety of protein prenyltransferases and other enzymes that employ FPP or

26 f the malaria parasite and mammalian protein prenyltransferases and their cellular substrates is impo

27 It aims at making prenyltransferases and their highly regio- and stereosel

28 to classify known protein substrates of CaaX prenyltransferases and to generate a list of hypothetica

29 proteins showed some similarity to existing prenyltransferases, and both contained a plastid-targeti

30 ted as prenyl acceptors in assays with three prenyltransferases, and we were able to detect turnover

31 ray crystallographic analysis with a protein prenyltransferase are described, verifying this compound

32 Protein prenyltransferases are also essential for many fungal an

33 Aromatic prenyltransferases are an actively mined enzymatic class

34 The protein prenyltransferases are heterodimers.

35 rved bio-chemical activities, we expect that prenyltransferases are likely integrated with the sterol

36 Prenyltransferases are widely distributed in prokaryotes

37 ence for the involvement of a plastidial cis-prenyltransferase (AtCPT7) in polyprenol synthesis.

38 gnaling, did not alter insulin action on the prenyltransferases, but completely inhibited the insulin

39 blocked by perillyl alcohol, an inhibitor of prenyltransferases, but not by the geranylgeranyltransfe

40 n a similar range to that of other flavonoid prenyltransferases, but the apparent catalytic efficienc

41 d and altering the substrate preference of a prenyltransferase by mutagenesis.

42 The cyanobactin prenyltransferases catalyze a series of known or unprece

43 Chain elongation prenyltransferases catalyze the addition of the hydrocar

44 Protein prenyltransferases catalyze the covalent attachment of i

45 Indole prenyltransferases catalyze the formation of a diverse s

46 Protein prenyltransferases catalyze the posttranslational modifi

47 CAAX motifs are substrates for the prenyltransferase-catalyzed addition of either farnesyl

48 Rubber transferase, a cis-prenyltransferase, catalyzes the addition of thousands o

49 is a strict requirement for the majority of prenyltransferases characterized to date.

50 cis-Prenyltransferases (cis-PTs) constitute a large family o

51 cis-Prenyltransferases (cis-PTs) constitute a large family o

52 nce in yeast and animals indicate that a cis-prenyltransferase (CPT) is required for dolichol synthes

53 In fact, genes encoding cis -prenyltransferase (CPT), a potential candidate for rubbe

54 e (rubber transferase; EC 2.5.1.20) is a cis-prenyltransferase (CPT).

55 cis-prenyltransferases (CPTs) are predicted to be involved i

56 These reactions are catalyzed by either cis-prenyltransferases (CPTs) or trans-prenyltransferases.

57 The aromatic prenyltransferase dimethylallyltryptophan synthase in Cl

58 mans, missense mutations to the protein UbiA prenyltransferase domain-containing 1 (UBIAD1) are respo

59 A prenyltransferase enzyme, UbiA prenyltransferase domain-containing 1 (UBIAD1), is invol

60 UBIAD1 (UbiA prenyltransferase domain-containing protein-1) utilizes

61 for hFTase (up to 333-fold) over the related prenyltransferase enzyme geranylgeranyltransferase-I (GG

62 A prenyltransferase enzyme, UbiA prenyltransferase domain-

63 is showed that it groups with a new class of prenyltransferase enzymes that lack the typical (N/D)DXX

64 This prenyltransferase exhibits strict substrate specificity

65 l extracts or recombinant N. tabacum protein prenyltransferases expressed in Escherichia coli.

66 ng with the characterization of two aromatic prenyltransferases, FamD1 and FamD2, and a previously un

67 CloQ belongs to the aromatic prenyltransferase family, which is characterized by an u

68 ns closely with that of the chain elongation prenyltransferase farnesyl diphosphate synthase rather t

69 t the relative importance of the two protein prenyltransferases, farnesyltransferase (FT) and geranyl

70 The tryptophan prenyltransferases FgaPT2 and 7-DMATS (7-dimethylallyl t

71 To explore the importance of the protein prenyltransferases for normal tissues, we used condition

72 ing mainly on terpenoid cyclases and related prenyltransferases for which X-ray crystal structures ha

73 CloQ is an aromatic prenyltransferase from the clorobiocin biosynthetic path

74 ents were performed with a number of protein prenyltransferases from different sources.

75 Membrane-embedded prenyltransferases from the UbiA family catalyze the Mg2

76 t insulin signaling from its receptor to the prenyltransferases FTase and GGTase I is mediated by the

77 The putative prenyltransferase gene ACLA_031240 belonging to the dime

78 a cluster of 10 additional genes including a prenyltransferase gene, ausN, located on a separate chro

79 In this study, an isoflavonoid prenyltransferase gene, designated as LaPT1, was identif

80 We identified a cis-prenyltransferase gene, neryl diphosphate synthase 1 (ND

81 thaliana mutants lacking functional protein prenyltransferase genes have also revealed roles for pre

82 only a few plant flavonoid and isoflavonoid prenyltransferase genes have been identified to date.

83 iptomic and metabolomic analyses to discover prenyltransferase genes in elicitor-treated peanut hairy

84 s because of its modification by the related prenyltransferase, geranylgeranyl:protein transferase ty

85 Using purified recombinant protein prenyltransferases, GST-lHDAg was found to be an excelle

86 , and the genes encoding stilbenoid-specific prenyltransferases have yet to be identified in any plan

87 dy provides a detailed characterization of a prenyltransferase in Malbranchea species, reveals two en

88 n summary, we have identified five candidate prenyltransferases in peanut and confirmed that two of t

89 iphosphate synthase belongs to a subgroup of prenyltransferases, including farnesyl diphosphate synth

90 ell growth through a mechanism not involving prenyltransferase inhibition.

91 by inhibiting oncogenic Ki-Ras through dual prenyltransferase inhibitor therapy.

92 tations when ras processing was inhibited by prenyltransferase inhibitor treatment.

93 ting Gly12 to Arg mutation) treated with the prenyltransferase inhibitor, L-778,123.

94 Although treatment with prenyltransferase inhibitors and radiation resulted in a

95 formation that is critical for the design of prenyltransferase inhibitors as anti-cancer agents.

96 s further demonstrate the potential of using prenyltransferase inhibitors in combination with radioth

97 certain prenyl alcohol analogues can act as prenyltransferase inhibitors in situ, via a novel prodru

98 The antimalarial actions of prenyltransferase inhibitors indicate that protein preny

99 by blocking Ras processing using a panel of prenyltransferase inhibitors of differing specificity fo

100 radiation survival in all cells and because prenyltransferase inhibitors target molecules other than

101 their H- or K-ras genes after treatment with prenyltransferase inhibitors that prevent the posttransl

102 The prenyltransferase inhibitors themselves inhibited clonog

103 enylation, and the ability of peptidomimetic prenyltransferase inhibitors to block parasite different

104 P-1 secretion can be regulated by statins or prenyltransferase inhibitors via effects mediated by ERK

105 In addition, prenyltransferase inhibitors which inhibit ras proteins

106 indicate the regions where specific protein prenyltransferase inhibitors with antifungal activity ca

107 latest discoveries related to the targets of prenyltransferase inhibitors.

108 The structure of MoeN5, a unique prenyltransferase involved in the biosynthesis of the an

109 from farnesyltransferase, another family of prenyltransferases involved in protein modification.

110 Whereas the Ram1p/Ram2p prenyltransferase is a cytosolic soluble enzyme, sequenc

111 cks the conserved motifs of conventional cis-prenyltransferase is required for natural rubber biosynt

112 ansferase type-I (GGTase-I), one of two CaaX prenyltransferases, is an essential enzyme in eukaryotes

113 We have characterized a new prenyltransferase isolated from genomic DNA of Malbranch

114 llographic structure of the "head-to-middle" prenyltransferase, isosesquilavandulyl diphosphate synth

115 similar to other flavonoid and isoflavonoid prenyltransferases; it has a predicted chloroplast trans

116 Here, we report that an unusual cis-prenyltransferase-like 2 (CPTL2) that lacks the conserve

117 UbiX acts as a flavin prenyltransferase, linking a dimethylallyl moiety to the

118 Kinetic crystallography reveals that the prenyltransferase mechanism of UbiX resembles that of th

119 found to possess 1,4-dihydroxy-2-naphthoate prenyltransferase (MenA) and bacterial electron transpor

120 ve organisms, the 1,4-dihydroxy-2-naphthoate prenyltransferase (MenA) inhibitors 1a and 2a act as sel

121 Here, 1,4-dihydroxy-2-naphthoate prenyltransferase (MenA) was targeted to reduce methicil

122 Two prenyltransferases modify CAAX proteins: farnesyltransfe

123 sma membrane location was blocked in vivo in prenyltransferase mutants and by mevinolin, which inhibi

124 ome of the phenotypes of Arabidopsis protein prenyltransferase mutants.

125 Orf2, a recently identified prenyltransferase of aromatic natural products, displays

126 no acid residues highly conserved with other prenyltransferases of the aromatic type.

127 Prenyltransferases of the dimethylallyltryptophan syntha

128 both GGPP and FPP compete for the binding to prenyltransferases, PGGT-I and PFT will likely be bound

129 we also examined the localization of protein prenyltransferases (PPTases) and regulation of GSIS by P

130 id (prenylation), is carried out by the CaaX prenyltransferases, protein farnesyltransferase (FTase)

131 re bifunctional, containing C-terminal trans-prenyltransferase (PT) and N-terminal terpene synthase (

132 The prenyltransferase (PTase) gene vrtC was proposed to be i

133 A small number of aromatic prenyltransferases (PTases) responsible for prenyl group

134 We found that in contrast to other protein prenyltransferases, RabGGTase is autoinhibited through N

135 In this assay, the PPi group produced in the prenyltransferase reaction is rapidly cleaved by inorgan

136 of diphosphate (PPi) dissociation during the prenyltransferase reaction under both single and multipl

137 FTase and GGTase-I (also called the CaaX prenyltransferases) recognize protein substrates with a

138 filled the last gap in the toolbox of indole prenyltransferases regarding their prenylation positions

139 These structures reveal that all protein prenyltransferases share a common reaction mechanism and

140 ther delineate the sequence requirements for prenyltransferase specificity and functional roles for p

141 report a chemoinformatic pipeline to enable prenyltransferase substrate prediction.

142 These results improve the definition of prenyltransferase substrate specificity, test the effica

143 for high-throughput screening for potential prenyltransferase substrates and inhibitors.

144 tive evidence for an additional two probable prenyltransferase substrates.

145 recently been characterized, namely the cis-prenyltransferases (TbCPTs) and the small rubber particl

146 en identified in the gene encoding UBIAD1, a prenyltransferase that synthesizes vitamin K2.

147 ss is catalyzed by a class of enzymes called prenyltransferases that are being intensively studied du

148 me, an archetypal member of a superfamily of prenyltransferases that generates lipophilic aromatic co

149 ibitors of differing specificity for the two prenyltransferases that modify K-Ras.

150 inhibit the consumption of DMADP and IDP by prenyltransferases to gain insight into the extent of en

151 In contrast to other prenyltransferases, UbiX is metal-independent and requir

152 The KgpF prenyltransferase was overexpressed and was shown to pre

153 Each of these prenyltransferases was highly specific for stilbenoid su

154 tagged and native Escherichia coli MiaA tRNA prenyltransferase, which uses dimethylallyl diphosphate

155 arison of the kinetic behavior of the parent prenyltransferases with that of the hybrid enzyme reveal