ライフサイエンスコーパス: preproinsulin

1 sulin expression on CD8+ T cell responses to preproinsulin.

2 tive differences in CD8+ T cell responses to preproinsulin.

3 o a normally stable reporter gene coding for preproinsulin.

4 to diabetes of NOD mice and suggest that the preproinsulin 1 gene is crucial for the spontaneous deve

5 e, tolerance to transgenically overexpressed preproinsulin 2 substantially reduced the onset and seve

6 In primary hepatocytes the presence of the preproinsulin 3'-UTR led to reduced mRNA levels compared

7 These studies revealed that the preproinsulin 5'-UTR was necessary for glucose stimulati

8 oinsulin protein enhance immune tolerance to preproinsulin, a key autoantigen in type 1 diabetes path

9 We find that whereas preproinsulin-A(SP23)S is efficiently cleaved, producing

10 Preproinsulin-A(SP24)D also blocks ER exit of coexpresse

11 producing authentic proinsulin and insulin, preproinsulin-A(SP24)D is inefficiently cleaved at an im

12 increased expression of ER-oxidoreductin-1, preproinsulin-A(SP24)D remains blocked but oxidative fol

13 nsulin transgene, alanine at position B16 in preproinsulin (B16:A-dKO mice), do not develop diabetes.

14 prediction algorithms, we identified GAD and preproinsulin candidate epitopes.

15 y better agonists than the wild-type "index" preproinsulin-derived peptide (ALWGPDPAAA) were identifi

16 to type 1 diabetes by regulating intrathymic preproinsulin expression.

17 strategy by which the T1D-triggering antigen preproinsulin fused with the immunoglobulin (Ig)G Fc fra

18 hannel subunit genes (ABCC8 and KCNJ11), and preproinsulin gene (INS) and investigated associations b

19 te that leptin inhibits transcription of the preproinsulin gene by altering transcription factor bind

20 but with little or no change in the level of preproinsulin gene expression.

21 crease in hexokinase activity, and increased preproinsulin gene transcription were observed in islets

22 cted changes in gene expression, for example preproinsulin gene, global changes in gene expression co

23 L30M preproinsulin-GFP fluorescence largely associated with t

24 site-directed mutagenesis of cDNA encoding a preproinsulin-green fluorescent protein (GFP) (C-peptide

25 e semi-nested products were characterized as preproinsulin I and II by restriction enzyme digestion a

26 The neurons showed the presence of preproinsulin I and II mRNA using polymerase chain react

27 Thus, we demonstrated the presence of preproinsulin I and II mRNA, insulin receptor mRNA and i

28 owed a band that co-migrated with pancreatic preproinsulin I and II mRNAs, and confirmed the PCR resu

29 ase protection assay using specific cRNA for preproinsulin I and II showed a band that co-migrated wi

30 Preproinsulin I mRNA was present in the 17 and 19 day ge

31 sulin II mRNA was shown within the brain and preproinsulin I mRNA within the retina.

32 re assessed in NOD mice that expressed mouse preproinsulin II from a transgene in K cells and nontran

33 Preproinsulin II mRNA was present in all the gestational

34 In the rat, preproinsulin II mRNA was shown within the brain and pre

35 demonstrate p97's role in pQC processing of preproinsulin in cases of naturally occurring mutations

36 os of circulating unmethylated to methylated preproinsulin (INS) DNA have been suggested to reflect b

37 Heterozygous mutations in the human preproinsulin (INS) gene are a cause of nonsyndromic neo

38 gment of the 5' untranslated region (UTR) of preproinsulin (Ins2) mRNA.

39 The mutations are in critical regions of the preproinsulin molecule, and we predict that they prevent

40 beta-cell line INS-1 on leptin exposure when preproinsulin mRNA expression is stimulated by 25 mM glu

41 ximately 90%, but they showed no decrease in preproinsulin mRNA expression.

42 eterminants in the 3'-UTR that stabilize the preproinsulin mRNA in a pancreatic beta-cell-specific ma

43 is via stimulation of the translation of the preproinsulin mRNA in pancreatic beta-cells.

44 Preproinsulin mRNA increased 64% without leptin and rose

45 A fall in levels of preproinsulin mRNA is detected in vivo in islets of ob/o

46 te that long term nutritional state sets the preproinsulin mRNA level in the beta-cell at which trans

47 In the longer term, when preproinsulin mRNA levels have increased approximately 2

48 that palmitate decreases glucose-stimulated preproinsulin mRNA levels in isolated rat islets, an eff

49 ets to elevated levels of palmitate inhibits preproinsulin mRNA levels in the presence of high glucos

50 (insulin secretion, proinsulin translation, preproinsulin mRNA levels, and total protein synthesis)

51 g insulin secretion, proinsulin translation, preproinsulin mRNA levels, or total protein synthesis.

52 two- to threefold oleate-induced increase in preproinsulin mRNA levels, underscoring the importance o

53 ng glucose stimulation (24 h) also increases preproinsulin mRNA levels.

54 iazoxide does not alter leptin inhibition of preproinsulin mRNA levels.

55 indicating that regulated translation of the preproinsulin mRNA occurs in a pancreatic beta-cell-spec

56 Thus, the untranslated regions of the preproinsulin mRNA play crucial roles in regulating insu

57 Overproduction of NO was reduced, and the preproinsulin mRNA response to free fatty acids was rest

58 an effect that is not mediated by changes in preproinsulin mRNA stability, but is associated with inh

59 UTR was necessary for glucose stimulation of preproinsulin mRNA translation, whereas the 3'-UTR appea

60 Preproinsulin mRNA was 2.9-fold above control levels, an

61 Also, pancreatic preproinsulin mRNA was completely absent in these icv le

62 ions, the untranslated regions (UTRs) of the preproinsulin mRNA were examined for elements that speci

63 ng recombinant adenoviruses that express the preproinsulin mRNA with defined alterations, the untrans

64 ing the translation and the stability of the preproinsulin mRNA.

65 e present study demonstrates the presence of preproinsulin mRNAs within the 15, 17 and 19 day gestati

66 concentrations did not alter MIN6 cell total preproinsulin, PC2, or PC3 mRNA levels.

67 ls, two epitopes, DR0401-restricted modified preproinsulin peptide 78-90(K88S) and zinc transport 8 2

68 igen HLA-A*0201-restricted glucose-sensitive preproinsulin peptide by the autoreactive TCR 1E6.

69 ding HLA class II and I molecules binding to preproinsulin peptides and T cell receptors, T and B cel

70 5 selected CD4 TCRs tested for reactivity to preproinsulin peptides presented by diabetes-susceptible

71 epitope derived from the leader sequence of preproinsulin (PPI) and show that 50% of HLA-A2+ patient

72 hat HLA-A24 molecules on islet cells present preproinsulin (PPI) peptide epitopes to CD8 cytotoxic T

73 itopes, monocyte-derived DC were pulsed with preproinsulin (PPI), glutamic acid decarboxylase (65-kDa

74 HLA-DQ2, -DQ8, and -DQ2/8 by pulsing DC with preproinsulin (PPI), IA-2, and GAD65.

75 complex class II null background, with human preproinsulin (PPI), proinsulin (PI), and insulin and de

76 -A24 (A*2402)-restricted epitope (peptide of preproinsulin [PPI](15-24), ALWGPDPAAA; or PPI(3-11), LW

77 We have shown that the injection of preproinsulin (ppins)-expressing pCI/ppins vector into P

78 mRNA in thymus such that elevated levels of preproinsulin protein enhance immune tolerance to prepro

79 udied multiligand discrimination by a human, preproinsulin reactive, MHC class-I-restricted CD8+ T ce

80 a-cell genes, particularly of those encoding preproinsulin, requires an appropriate euchromatin (or "

81 Recently, missense mutations upstream of preproinsulin's signal peptide (SP) cleavage site were r

82 ta suggest that INS-IGF2, which contains the preproinsulin signal peptide, the B-chain, and eight ami

83 INS-IGF2 consists of the preproinsulin signal peptide, the insulin B-chain, and e

84 Using type 1 diabetes patient-derived preproinsulin-specific CD8 T-cell clones recognizing eit