ライフサイエンスコーパス: prereplicative

1 NEIL1 was recently demonstrated to initiate prereplicative base excision repair (BER) of oxidized ba

2 eplication by mutations in components of the prereplicative complex (origin recognition complex [ORC]

3 rate that these MCM2-7 mutants arrest during prereplicative complex (pre-RC) assembly after OCM forma

4 In vivo, depletion of Orc6 inhibits prereplicative complex (pre-RC) assembly and maintenance

5 The first step is the formation of a large prereplicative complex (pre-RC) at individual replicatio

6 tion requires the assembly of a multiprotein prereplicative complex (pre-RC) at the origins.

7 phosphorylation of Hbo1 may be required for prereplicative complex (pre-RC) formation and DNA replic

8 nents of the ORC complex and Cdc6 stimulated prereplicative complex (pre-RC) formation and replicatio

9 gulation is thought to involve inhibition of prereplicative complex (pre-RC) formation at origins by

10 irus-based small interfering RNA techniques, prereplicative complex (pre-RC) formation is disrupted a

11 mplexes that are assembled on the DNA during prereplicative complex (pre-RC) formation.

12 s loaded onto chromatin in the final step of prereplicative complex (pre-RC) formation.

13 vity, an extended protein complex called the prereplicative complex (pre-RC) forms over the origin of

14 Here, we report that depletion of the prereplicative complex (pre-RC) protein Cdc6 causes huma

15 nd to origins of DNA replication and recruit prereplicative complex (pre-RC) proteins, one of which i

16 Replication initiation proteins, known as prereplicative complex (pre-RC) proteins, serve as a pri

17 -2-7 loading in late M phase depended on the prereplicative complex (pre-RC) proteins: origin recogni

18 During G1 phase, a prereplicative complex (pre-RC) that determines where DN

19 ccomplished by controlling the assembly of a prereplicative complex (pre-RC) which involves the seque

20 in that is required for the formation of the prereplicative complex (pre-RC), an essential replicatio

21 resulting protein-DNA assembly is called the prereplicative complex (pre-RC), and its formation requi

22 MCM2-7 is a key component of the prereplicative complex (pre-RC), which is loaded onto ch

23 rdinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC).

24 omosome maintenance (MCM) proteins to form a prereplicative complex (pre-RC).

25 ORC participates in the formation of the prereplicative complex [3], which is necessary to establ

26 n fission yeast that delays formation of the prereplicative complex at chromosomal replication origin

27 de binding is important for establishing the prereplicative complex at origins of DNA replication and

28 hat Cdc23p participates in the activation of prereplicative complex by recruiting the Dfp1-Hsk1 kinas

29 DNA synthesis-coupled proteolysis of the prereplicative complex component Cdt1 by the CRL4(Cdt2)

30 plication initiates relative to the sites of prereplicative complex formation are not known.

31 leosomes positioned by ORC are important for prereplicative complex formation.

32 rengthen the observation that members of the prereplicative complex have multiple functions and provi

33 Upon checkpoint activation, a prereplicative complex is assembled that contains ORC, C

34 he MCM complex to origins in G1 as part of a prereplicative complex is critical for the cell cycle re

35 hrough binding directly to components of the prereplicative complex thereby promoting DNA synthesis,

36 These mutants inhibit formation of the prereplicative complex when overexpressed.

37 Cdc6p is part of the prereplicative complex, which is essential for DNA repli

38 nding pad for the assembly of a multiprotein prereplicative complex, which is required to initiate DN

39 ins MCM proteins and therefore resembles the prereplicative complex.

40 nance protein 2 (MCM2) is a component of the prereplicative complex.

41 the G1 cyclin Cln2 inhibits the assembly of prereplicative complexes (pre-RCs) and induces gross chr

42 ps: the Mcm2-7 helicase is first loaded into prereplicative complexes (pre-RCs) as an inactive double

43 The assembly of essential prereplicative complexes (pre-RCs) at origins only occur

44 e multiple mechanisms to block reassembly of prereplicative complexes (pre-RCs) at replication origin

45 on complex (ORC) is involved in formation of prereplicative complexes (pre-RCs) on replication origin

46 Assembly of these prereplicative complexes (pre-RCs) requires the Origin R

47 To analyze the assembly and activation of prereplicative complexes (pre-RCs), we tested the effect

48 hromosome maintenance proteins (Mcm2-7) into prereplicative complexes (pre-RCs).

49 per cell cycle by preventing the assembly of prereplicative complexes (pre-RCs; licensing) outside of

50 Mcm2-7 family members (the MCM complex) into prereplicative complexes at budding yeast origins of DNA

51 nichromosome maintenance (MCM) proteins form prereplicative complexes at origins of replication.

52 The timely assembly of prereplicative complexes at replication origins is tight

53 , cells prepare for initiation by assembling prereplicative complexes at their replication origins.

54 ation-licensing factors are required to form prereplicative complexes during G1 phase, but are inacti

55 c6 and Cdt1 are required for the assembly of prereplicative complexes during G1 phase.

56 ing of these processes from the formation of prereplicative complexes in preparation for S phase to t

57 censing system ensures that the formation of prereplicative complexes is temporally separated from th

58 cycle-dependent, ordered assembly of protein prereplicative complexes suggests that eukaryotic replic

59 using human Cdc6, an essential component of prereplicative complexes, as bait.

60 eplication origins after the assembly of the prereplicative complexes.

61 accumulate in intranuclear speckled punctate prereplicative foci, some of which colocalize with numer

62 H in BALB/c mice, p21 was induced during the prereplicative (G1) phase and was maximally expressed af

63 highest levels during the quiescent (G0) and prereplicative (G1) phases, and its degradation is requi

64 e of chemical DNA modifications that reverse prereplicative guanine methylation.

65 At the checkpoint between the prereplicative phase of growth and the phase of chromoso

66 mbiguously established, even in the event of prereplicative recombinational exchanges, by haplotype a

67 zed base during replication, NEIL1 initiates prereplicative repair by acting as a "cowcatcher" and pr

68 esults support a mechanism of NEIL1-mediated prereplicative repair of oxidized bases in the replicati

69 d templates in mammalian genomes, warranting prereplicative repair of the mutagenic base lesions.

70 its recruitment to the replication site for prereplicative repair was not investigated.

71 , because the viral protein requirements for prereplicative site formation are reduced in transfected

72 sis of these results, we present a model for prereplicative site formation in infected cells in which

73 To determine if prereplicative site formation is dependent upon these an

74 tigated the requirement for formation of the prereplicative site pattern by using double mutants of H

75 ve in both UL30 and UL5, we suggest that the prereplicative site pattern can form under conditions in

76 t neither viral helicase is required for the prereplicative site pattern to form as long as a polymer

77 tage II) and the formation of PML-associated prereplicative sites (stage III) and replication compart

78 myelocytic leukemia protein (PML)-associated prereplicative sites and contain all seven replication p

79 The d105 ICP8 protein blocks formation of prereplicative sites and large replication compartments

80 of preventing the formation of intranuclear prereplicative sites and replication compartments during

81 the recruitment of PML isoforms to stage III prereplicative sites and replication compartments requir

82 hich other proteins can be recruited to form prereplicative sites and replication compartments.

83 ctions may be important for the formation of prereplicative sites and replication compartments.

84 DNA binding protein ICP8 in the formation of prereplicative sites and replication compartments.

85 ed-cell nucleus, leading to the formation of prereplicative sites and replication compartments.

86 at the helicase-primase complex localizes to prereplicative sites and replication compartments.

87 ce of the viral polymerase and/or UL9 within prereplicative sites and the integrity of ND10.

88 iral replication proteins in the assembly of prereplicative sites are presented.

89 il to express the polymerase protein contain prereplicative sites in the absence and presence of poly

90 ssary for the localization of ICP8 (UL29) to prereplicative sites natural infection conditions.

91 ruption of ND10 and display nuclear foci, or prereplicative sites, containing the viral single-strand

92 PA) are recruited to the earliest detectable prereplicative sites, stage II microfoci.

93 uit the polymerase catalytic subunit UL30 to prereplicative sites, suggesting that an active primase,

94 edominantly in stage IIIb but not stage IIIa prereplicative sites, suggesting that the efficient recr

95 teins localize to punctate structures called prereplicative sites.

96 e viral polymerase prevents the formation of prereplicative sites.

97 itors, mutant-infected cells contain UL29 in prereplicative sites.

98 UL2 co-localizes with UL30 to nuclear viral prereplicative sites.

99 ation proteins at small punctate foci called prereplicative sites.

100 erous punctate nuclear foci which are called prereplicative sites.

101 localization of the viral DNA polymerase to prereplicative sites.

102 otein in the assembly of these proteins into prereplicative sites.

103 29 localizes to punctate nuclear foci called prereplicative sites.

104 ed punctate nuclear structures that resemble prereplicative sites; however, the FNF and FW mutants fa

105 Furthermore, there were discrete G1 phase (prereplicative stage in the cell cycle) abnormalities in

106 istent with a possible role for Cdc68 in the prereplicative stage of the cell cycle.

107 s are maintained in the initiation-competent prereplicative state for extended periods.