ライフサイエンスコーパス: presequence

1 a preprotein with a 50-amino acid N-terminal presequence.

2 its native form by removal of the N-terminal presequence.

3 and it recognizes a mitochondrial targeting presequence.

4 s of mature QPs3 and a 55-amino acid residue presequence.

5 ed to a size consistent with cleavage of the presequence.

6 includes a putative chloroplastic targeting presequence.

7 ibiting a step other than proteolysis of the presequence.

8 matrix, based on an N-terminal, amphipathic presequence.

9 and function of the 20-amino acid N-terminal presequence.

10 l step in import, i.e., translocation of the presequence.

11 rane by a bipartite, cleaved, amino-terminal presequence.

12 ytoplasm without a conventional mt-targeting presequence.

13 uits other motor subunits and binds incoming presequence.

14 ed M(r) of 52,000 containing a 31-amino acid presequence.

15 ssessing independent mitochondrial targeting presequences.

16 residues and contain 9-amino-acid cleavable presequences.

17 inner membrane Tim17/23 translocon by their presequences.

18 ns targeted by positively charged N-terminal presequences.

19 (1) protein has been shown to lack a cleaved presequence; a single methionine is removed from the ami

20 teins carry a positively charged, N-terminal presequence and are imported by the TIM23 complex (prese

21 c, allowing recognition of both the incoming presequence and other translocase components at the tran

22 ows that MPP recognizes a second site in the presequence and processing occurs between residues 43 an

23 is not imported using a conventional cleaved presequence and show that sequences at the N-terminus of

24 efore MIP cleaves off the second part of the presequence and that this second processing step takes p

25 oteins of the intermembrane space (IMS) lack presequences and are imported in an oxidation-driven rea

26 e two major IMS-targeting signals, bipartite presequences and cysteine motifs.

27 d us to propose that the length of targeting presequences and the subunit composition of organellar p

28 s are targeted to mitochondria by N-terminal presequences and use the TIM23 complex for their translo

29 independent of proteolytic processing of the presequence, and that o-phenanthroline together with EDT

30 tructure unveils how mitochondrial targeting presequences are recognized.

31 These presequences are rich in positive charges, mainly argini

32 reveals the highly conserved nature of such presequences, as well as of the import machinery.

33 The cleavable presequence at the amino terminus of the precursor form

34 the Deltapsi is not linked to the respective presequence, but rather to the mature portion of the pol

35 ired in TOM-TIM23 coupling and the import of presequence-carrying preproteins.

36 can assume a conformation incompatible with presequence cleavage.

37 al peptide are important for Som1p-dependent presequence cleavage; however, no specific cargo sequenc

38 ing plasmids lacking either the IDH1 or IDH2 presequence coding regions were unexpectedly found to be

39 ecursors have functionally similar bipartite presequences composed of an N-terminal signal peptide do

40 ing experiments indicate that the N-terminal presequence-containing domain of the substrate proteins

41 Here, we report that import of presequence-containing precursors and carrier proteins i

42 0 along with Tim23 regulate translocation of presequence-containing proteins across the mitochondrial

43 As shown herein, the presequence contains significant secondary structure and

44 ransit peptides, and processing of the LAP-N presequence could generate the mature 55-kD LAP-N.

45 Only 24% of carriers met EHR-based presequencing criteria for probable or definite FH diagn

46 sparagine-linked glycosylation reporters and presequence deletion constructs of the precursor to the

47 , while shorter hydrogenosomal and mitosomal presequences did not.

48 al target presequence of 64 residues and the presequence directed the green fluorescent protein to to

49 The bipartite presequences directing the products of these genes back

50 The presequence dramatically altered SCP-2 structure as dete

51 x) sorts precursor proteins with a cleavable presequence either into the matrix or into the inner mem

52 We propose that the presequence either targets Atp6p to the Atp9p or signals

53 The 16-amino acid mitochondrial targeting presequence, encoded by the second and third exons, was

54 h subunit was found to be dependent upon its presequence for mitochondrial localization, and the subu

55 gulator2 homolog (SRT2) contains a predicted presequence for mitochondrial targeting.

56 her CCS nor SOD1 contains typical N-terminal presequences for mitochondrial uptake; however, the mito

57 ity of mitochondrial proteins use N-terminal presequences for targeting to mitochondria and are trans

58 id N-terminal presequence that is similar to presequences found on all examined hydrogenosomal protei

59 dria, even after fusion of the mitochondrial presequence from pmAAT to its N-terminal end.

60 -directed mutagenesis was used to remove the presequences from each gene and from both genes carried

61 st three peptidases are involved in cleaving presequences from imported mitochondrial proteins.

62 was shown by expressing the LipDH N-terminal presequences fused to green fluorescent protein in eryth

63 ssed a natural substrate, pre-23K, and a 23K presequence-green fluorescent protein (GFP) chimera in v

64 The LAP-A presequence had features similar to chloroplast transit

65 The 5-kD presequence had features similar to plastid transit pept

66 ex we used iron-sulfur proteins in which the presequences had been changed by site-directed mutagenes

67 nosomal AAC contains a cleavable, N-terminal presequence; however, this sequence is not necessary for

68 ta indicate the importance of the N-terminal presequence in regulating SCP-2 structure, cholesterol l

69 his study has examined the role of the Atp6p presequence in the function and assembly of the ATPase c

70 PP), is capable of cleaving a hydrogenosomal presequence in vitro, in contrast to MPP which requires

71 of Sf9 cells and required the mitochondrial presequences in both subunits.

72 ial proteins are synthesized with N-terminal presequences in the cytosol.

73 sized as preproteins carrying amino-terminal presequences in the cytosol.

74 studies of Pex5p(C) binding], the N-terminal presequence increased SCP-2's affinity for Pex5p(C) by 1

75 Inspection of the LAP-A presequence indicated the presence of a dibasic protease

76 Translocation of the presequence is an early event in import of preproteins a

77 rt competence can be retained as long as the presequence is capable of forming a relatively more stab

78 If proline-20 of the S. pombe presequence is changed into a serine, a second cleavage

79 onversely, if serine-24 of the S. cerevisiae presequence is changed to a proline, the first cleavage

80 The mutated presequence is cleaved and forms mature-sized protein in

81 uggesting that the N-terminal portion of the presequence is essential for membrane binding.

82 surface is dramatically accelerated when its presequence is long enough to span both membranes and to

83 cates that the precursor of Tic22 (preTic22) presequence is necessary and sufficient for targeting to

84 ges in the N-terminal helical segment of the presequence is necessary for import competence.

85 sults indicate that removal of the subunit 6 presequence is not an essential event for ATPase biogene

86 ate of DeltapH-driven translocation, and the presequence is optimized in its natural acidic residue c

87 icate that a single amino acid change in the presequence is responsible for one-step processing in S.

88 If the presequence is too short, import is slow but can be stro

89 rotein and the COOH-terminal residues of its presequence is used as a substrate.

90 d us to postulate that the N-terminus of the presequence is vital for import and the C-terminal end i

91 Specifically, longer mitochondrial presequences may have evolved to require an alpha/beta h

92 long 105-amino acid mitochondrial targeting presequence (mTP).

93 domain hydrophobic region oriented with the presequence N terminus formed by signal peptidase cleava

94 (NTD) is intrinsically disordered and binds presequence near a region important for interaction with

95 presequence of the ribosomal protein L29 and presequence of 1-aminocyclopropane-1-carboxylic acid dea

96 rame of 2634 nucleotides encoding a putative presequence of 31 amino acid residues and a mature prote

97 A peptide presequence of 52 amino acids is cleaved to give the mat

98 ed a typical N-terminal mitochondrial target presequence of 64 residues and the presequence directed

99 lacking negative charges in the unstructured presequence of LF(N) translocate independently of the De

100 The latter effect depends on the presequence of LMP7, but not on LMP7 catalytic activity.

101 st for the first time that the 20-amino acid presequence of pro-SCP-2 alters SCP-2 structure to facil

102 these positive charges in the 19-amino acid presequence of rat liver aldehyde dehydrogenase was inve

103 from the first 25 amino acid residues in the presequence of subunit IV of yeast cytochrome c oxidase

104 The mitochondrial targeting presequence of the "Rieske" protein (subunit 9) is lodge

105 N- and C-terminal fragments derived from the presequence of the ATPase beta subunit ranging in size f

106 We previously reported that the presequence of the F(1)-ATPase beta subunit precursor (p

107 e cytochrome bc1 complex, we mutagenized the presequence of the iron-sulfur protein to eliminate the

108 s, respectively, in the cleaved NH2-terminal presequence of the precursor protein (pre-LMP3), and six

109 Short mitochondrial targeting peptides (presequence of the ribosomal protein L29 and presequence

110 asts of a chimeric protein consisting of the presequence of the small subunit of ribulose 1,5-bisphos

111 imeric precursor proteins, consisting of the presequence of the small subunit of Rubisco fused to the

112 ue peptide derived from the membrane-binding presequence of yeast cytochrome c oxidase, and the pepti

113 On the basis of the unique presequences of the nuclear genes and the recent mitocho

114 In contrast, the mitochondrial targeting presequences of these same proteins have not undergone a

115 The mitochondrial targeting presequences of two sdh3 genes are derived from preexist

116 rt rates of artificial precursors containing presequences of varying length fused to either mouse dih

117 ese observations suggest that the N-terminal presequence on the avidin-bound precursor is available f

118 ed question in gene evolution: the origin of presequences or transit peptides that generally exist in

119 hierarchical dominance of the mitochondrial presequence over the NLS.

120 The protein analytes were positively charged presequences (pb2) of varying length fused to the small

121 f mitochondrial Abeta-degrading enzyme PreP (presequence peptidase) was enhanced in Tg mAPP mitochond

122 etic peptide corresponding to the 29-residue presequence peptide (mAAT-pp) with anionic phospholipid

123 could be re-assigned and characterized as a presequence peptide degrading enzyme in the matrix.Prote

124 om40 constructs specifically interact with a presequence peptide in a concentration- and voltage-depe

125 sistent with only a shallow insertion of the presequence peptide in the bilayer.

126 supported by the fact that the addition of a presequence peptide induces transient pore closure.

127 Based on these results, we propose that the presequence peptide may contain dual recognition element

128 no acid sequence, indicating a mitochondrial presequence peptide of only nine amino acids.

129 channel in the presence of the mitochondrial presequence peptide pF(1)beta revealed the kinetics of p

130 nnel formation or for the interaction with a presequence peptide.

131 o not, however, alter the activity of PSC, a presequence-peptide sensitive channel in the mitochondri

132 Cleaved presequence peptides then need to be efficiently degrade

133 s not significantly blocked by mitochondrial presequence peptides.

134 uded that positively charged residues in the presequence play a vital role in the import of precursor

135 m the mature protein portion and less on the presequence portion, when a synthetic peptide composed o

136 Analysis of the presequence predicts that the protein is targeted to out

137 Although the 20-amino acid presequence present in 15-kDa pro-sterol carrier protein

138 ure the correct functioning of the essential presequence processing machinery.

139 Presequence protease (PreP), a mitochondrial peptidasome

140 gradation of targeting peptides catalyzed by presequence protease.

141 A role for the cargo in presequence recognition distinguishes Imp1p and Imp2p fr

142 otosystem II (pLHCPII) were used to identify presequence regions translocated into the ER lumen and s

143 y brominated phospholipids reveals that this presequence residue inserts to a depth of approximately

144 of the peptide corresponding to the R3Q/R10Q presequence revealed that this peptide was only somewhat

145 of hydrogenosomal and mitochondrial protein presequences reveals striking similarities.

146 These results suggest that the presequence segment lies close to the surface of the mem

147 peptide corresponding to the linker-deleted presequence showed that it was substantially more prone

148 Analysis of presequence structure and in vitro import experiments in

149 Analysis of presequence structure and in vitro import into microsoma

150 They have an N-terminal presequence that allows recognition by the mitochondrial

151 to precursors with an NH2-terminal targeting presequence that are imported in a linear NH2-terminal m

152 ine-rich, 69-residue mitochondrial targeting presequence that is absent in the mature protein.

153 synthesized with an N-terminal 10-amino acid presequence that is cleaved during assembly of the compl

154 e presence of an eight amino acid N-terminal presequence that is similar to presequences found on all

155 as a preprotein with a cleavable N-terminal presequence that is the mitochondrial targeting signal,

156 ino-acid amino-terminal, organelle-targeting presequence that was cleaved in vivo.

157 their respective organelles using N-terminal presequences that are subsequently cleaved by a peptidas

158 tions of three peptides, which correspond to presequences that direct mitochondrial protein import, w

159 interaction, including the length of the pb2 presequence, the position of the electrostatic traps wit

160 Tim23p does not carry an amino-terminal presequence; therefore, the targeting information reside

161 ith the structural similarity of all Euglena presequences, these results demonstrate that chloroplast

162 to drive translocation of positively charged presequences through the TIM23 complex (presequence tran

163 of a conserved leucine at position 2 in the presequence to a glycine disrupts import of pFd into the

164 ct on the affinity or mode of binding of the presequence to model membranes.

165 The presequence translocase (TIM23 complex) sorts precursor

166 The presequence translocase constituent Pam17 is specificall

167 nclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at eleva

168 The presequence translocase of the inner membrane (TIM23 com

169 of the outer mitochondrial membrane and the presequence translocase of the inner membrane.

170 The presequence translocase of the inner mitochondrial membr

171 uence and are imported by the TIM23 complex (presequence translocase) located in the inner mitochondr

172 rged presequences through the TIM23 complex (presequence translocase), the activity of the Hsp70-powe

173 chaperone cooperates with cochaperones, the presequence translocase, and other chaperone systems.

174 ates with partner protein complexes like the presequence translocase-associated import motor and the

175 a through interactions with the import motor presequence translocase-associated motor (PAM).

176 ormal channel activity and disruption of the presequence translocase-associated motor complex did not

177 from the channel to the import motor of the presequence translocase.

178 to mitochondria and are translocated by the presequence translocase.

179 nown energy-dependent steps: Deltapsi-driven presequence translocation and adenosine triphosphate-dri

180 ot modify TIM23 channel activity nor prevent presequence translocation.

181 With precursors that have sufficiently long presequences, unfolding by the inner membrane import mac

182 This presequence was necessary to target Hsp60 to one (and oc

183 , whose sequences mimic mitochondrial import presequences, was measured using a novel, qualitative, f

184 the putative mitochondrial matrix targeting presequences) were co-expressed in Escherichia coli on o

185 are imported from the cytosol via N-terminal presequences, which are cleaved upon exposure to the mit

186 east promoters and a mitochondrial targeting presequence with the bacterial coding sequence.