ライフサイエンスコーパス: primer

1 n priming mechanism using the TP domain as a primer.

2 roper generation of a polypurine tract (PPT) primer.

3 fficiently added to the growing end of a TNA primer.

4 nation and thus synthesis of the replication primer.

5 enature the duplex and thus expose a blocked primer.

6 e sugar-phosphate backbone of the DNA or RNA primer.

7 replication of mtDNA by generation of an RNA primer.

8 through design and efficient organization of primers.

9 variation, and efficiently pools compatible primers.

10 16S rRNA gene using Com1 and Com2 universal primers.

11 enome-level and efficiently pools compatible primers.

12 h hydrogel beads (HBs) bearing barcoding DNA primers.

13 al-time RT-PCR with bacterial group-specific primers.

14 es to the user's input, output, and designed primers.

15 tching, which generates capped transcription primers.

16 DNA polymerases can be inhibited by certain primers.

17 subspecies, produced amplicons with IS901PCR primers.

18 map of all inputted sequences with designed primers.

19 suitable for designing candidate IGG marker primers.

20 ification and validation of both samples and primers.

21 obtained from the oils and the amplification primers.

22 blished, the authors incorrectly stated that primers 28F-519R were reported in ref.

23 in a cost-effective manner, with sharing of primers across constructs allowing significant reduction

24 We designed 19 new specific primers anchored in a stepwise fashion in order to detec

25 We find that the binding of template-primer and key aspects of the RT active site are surpris

26 ion provides viral RNA-positive controls and primer and probe nucleotide sequences for real-time RT-P

27 In silico analyses of mapA and ceuE primer and probe sequences from 1,713 genetically divers

28 For this purpose, specific primers and a minor groove binding (MGB) TaqMan probe we

29 different omega- and omega-1-functionalized primers and alpha-functionalized extender units in combi

30 DNA sequencing with methylation-specific primers and cDNA analysis in patient neurons indicated s

31 -in-oil emulsion droplets in the presence of primers and dNTPs, followed by the recovery of the partn

32 which can accept a variety of functionalized primers and functionalized extender units and operate in

33 aryotic microbes: anneal-inhibiting blocking primers and peptide-nucleic acid (PNA) oligonucleotide b

34 quantifying HRV RNA using genotype-specific primers and probes and a consensus primer/probe set targ

35 When using consensus primers and probes for the quantification of HRV, RT-dPC

36 In this study, a new set of primers and probes was designed for one-step pan-IAV rev

37 Group-specific primers and probes were developed to detect numerous spe

38 specific factors, such as choice of adapters/primers and sample amplification methods.

39 mirror-image l-DNA analogs of the reaction's primers and targets.

40 employing two forward primers, three reverse primers, and four fluorophore-labeled probes in a single

41 ded DNA, and a suppression of non-homologous primer annealing and nonspecific amplification.

42 reaction contents and conditions that impact primer annealing and product melting and eliminates the

43 he cycling conditions required for effective primer annealing and product melting during each cycle w

44 n events during each cycle of amplification, primer annealing and product melting.

45 nts that variably inhibit Taq polymerase and primer annealing.

46 the contacts observed previously with an RNA primer are preserved with a DNA primer--with the same se

47 Barcoded adapter primers are designed with an oligonucleotide hairpin str

48 These primers are termed "swarm primers" based on their relati

49 ation multiplex PCR experiments that ensures primers are unique at a genome-level and efficiently poo

50 Thus, we developed Tetra-primer ARMS assays for rapid, reliable, reproducible and

51 de a facility for the user to draw their own primers as well as comprehensive visual guides to the us

52 e efficiency on AZT- or tenofovir-terminated primers, as compared with the double-mutant D67N/K70R.

53 TIMA CT or APTIMA GC, which target alternate primers, as the confirmatory tests.

54 operationalization of a process called Twin-Primer Assembly (TPA), which is a method to assemble pol

55 n mechanism, in which the 3'-hydroxyl of the primer attacks the phosphate of the incoming monomer, di

56 Ten pairs of species-specific primers based on the ITS2 rDNA were developed for psocid

57 pical bont tick (Amblyomma variegatum) using primers based on the variegin sequence, which we previou

58 These primers are termed "swarm primers" based on their relatively high concentration an

59 confirmed these results by using single-cell primer-based nested PCRs and Sanger sequencing.

60 E pipeline to improve analysis speed, reduce primer bias (requiring two sequencing primers), enhance

61 Metacoder also allows exploration of barcode primer bias by integrating functions to run digital PCR.

62 tivation (TAR), polyadenylation (PolyA), and primer binding (PBS) elements, do not participate in int

63 inal CCA of mature tRNAs and target the tRNA primer binding site (PBS) essential for ERV reverse tran

64 A binding to a double-stranded region of the primer binding site (PBS)-segment of the 5'-UTR.

65 ble A/B recombinants exhibiting DWV-B at PCR primer binding sites, may be a major cause of elevated o

66 0.3 M EDC for 1 min and then bonded with the primer-bonding agent.

67 G-rich inhibitory sequence-containing primers can be used in PCR at a lower concentration to a

68 ic 'blind spot' because of mismatches in the primers commonly used for ribosomal gene surveys.

69 The qPCR conditions, primer concentration and annealing temperature, were opt

70 Additionally, these species-specific primers could quantify specificity and identify 10 store

71 on of RNA synthesis, most RdRps use either a primer-dependent or de novo mechanism3.

72 Using a matching primer design algorithm it also suggests users possible

73 e a streamlined strategy, based on automated primer design and recombinational cloning, allowing one

74 he freely available Primerize-2D server, the primer design code is available as a stand-alone Python

75 Primer design represents a widely employed gambit in div

76 lectrophoresis are displayed as parts of the primer design result.

77 uence-complete genomes, comprising an online primer design tool, a novel multiplex PCR enrichment pro

78 mic mutations, the protocol-including target primer design, variant library construction, and sequenc

79 viral genomes using the online Primal Scheme primer designer software.

80 nt innovations, providing researchers with a primer detailing circuit mapping strategies in the cereb

81 An interactive primers diagram, a sequence alignment viewer and a virtu

82 stem was developed to minimize the effect of primer-dimer amplification on the assay.

83 viding the user with both, graphical maps of primer distribution for each inputted sequence, and also

84 ntron RT in complex with an RNA template-DNA primer duplex and incoming deoxynucleotide triphosphate

85 rating a single base mismatch in the growing primer duplex, which attenuates DNA charge transport, in

86 erage at the termini by introducing modified primers during the targeted amplification step to genera

87 3' terminus of DNA primers, enabling further primer elongation (excision mechanism).

88 STR-selective primers enable massively parallel, targeted sequencing o

89 es the inhibitor from the 3' terminus of DNA primers, enabling further primer elongation (excision me

90 reduce primer bias (requiring two sequencing primers), enhance species-level analysis, and add new vi

91 Here, we introduce the concept of primer exchange reaction (PER) cascades, which grow nasc

92 ranscript selective 2'-hydroxyl acylation by primer extension (SHAPE) chemical probing, we show that

93 selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) mapping.

94 selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) RNA structure probing.

95 mpute the selective 2' hydroxyl acylation by primer extension (SHAPE)-directed ensemble for the RNA f

96 selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) to

97 e structure and function of mt-tRNA(Asp) The primer extension assay demonstrated that the m.7551A > G

98 en fluorescent protein abundance, and blocks primer extension by DNA polymerase, thereby demonstratin

99 cesses including DNA strand displacement and primer extension by DNA polymerases that resulted in pre

100 A working model of nonenzymatic RNA primer extension could illuminate how prebiotic chemistr

101 substrates for DNA polymerases applicable in primer extension or PCR synthesis of modified oligonucle

102 The fidelity of the primer extension products resulting from the sequential

103 Fluorescent labeling of the primer extension products was achieved by fluorophores w

104 the second signal transduction step based on primer extension reaction coupled with TaqMan probe.

105 ior reaction kinetics and improved yields of primer extension reaction products.

106 l selective 2-hydroxyl acylation analyzed by primer extension sequencing (SHAPE-Seq) to simultaneousl

107 ity of the modified DNA has been verified by primer extension studies with DNA polymerases I and IV f

108 vious characterizations of template-directed primer extension using 5'-phosphoryl-2-methylimidazole-a

109 rimental reconstructions of nonenzymatic RNA primer extension yield a mixture of 2'-5' and 3'-5' inte

110 omer addition as well as trimer-assisted RNA primer extension, allowing efficient copying of a variet

111 2'-hydroxyl acylation with lithium ion-based primer extension, and identifies characteristic structur

112 modates RNA as one of the two strands during primer extension, mainly by inserting dNMPs opposite unm

113 ctions is Selective 2' Hydroxyl Acylation by Primer Extension, or SHAPE.

114 ication through these artificial linkages by primer extension, PCR, and deep sequencing reveals that

115 on efficiency but promotes limited rounds of primer extension.

116 Polymerase-mediated primer-extension assays reveal that tCfTP is efficiently

117 ne polymerase chain reaction with degenerate primers, followed by high-throughput sequencing to asses

118 An important primer for metaflammation is the persistent metabolic ov

119 replication.IMPORTANCE Human tRNA(Lys3), the primer for reverse transcription, and LysRS are essentia

120 w provides general information to serve as a primer for those embarking on understanding food allergy

121 Primers for 7 miRNA targets were orthogonally designed t

122 of wild-caught L. longipalpis using specific primers for a locus from the chloroplast genome, ribulos

123 gn algorithm it also suggests users possible primers for experimental validation by RT-PCR and displa

124 We designed primers for genes specific to S. Typhi, S. Paratyphi A,

125 e that automates the design of multiplex PCR primers for next-generation sequencing.

126 rm for researchers to automate the design of primers for overlapping PCR applications.

127 signed web tool that automates the design of primers for overlapping PCR.

128 aking it difficult to design genome-specific primers for sequence analysis.

129 an amplicon size of 530bp using the specific primers for shrimps, 16S-Cru4/16S-Cru3.

130 nic biomethylation, and developed degenerate primers for the amplification of arsM genes to identify

131 We designed HRM-specific primers for the Mytilus genus to identify M. chilensis,

132 However, the process of designing sets of primers for this method has many degrees of freedom and

133 ises a PCR system with four sets of specific primers, for each target species.

134 ingle-stranded DNA binding protein increases primer formation and extension efficiency but promotes l

135 chondrial D loop gene using species specific primers found 226bp and 126bp product amplicons for buff

136 graphical user interface that designs robust primers from any number of inputted sequences while prov

137 However, the mechanism of PPT primer generation and the features of the PPT sequence t

138 esults reveal an unexpected mechanism of PPT primer generation based on specific dynamic properties o

139 to process and analyze VCF files, including primer generation for variant validation, dendrogram pro

140 olecule assays to study the mechanism of PPT primer generation.

141 Escherichia coli and Klebsiella pneumoniae (PRIMERS I).

142 In PRIMERS I, the 4 RMD platforms detected beta-lactamase (

143 In this Primer, I summarise how this multifaceted gene functions

144 In this Primer, I will explain how perceptual learning is transf

145 Primer ID sequencing provides a novel approach to study

146 We used the Primer ID sequencing strategy with the MiSeq sequencing

147 in deep-sequencing technology combined with Primer ID-tagged cDNA primers to efficiently quantify HI

148 We developed an assay using Primer ID-tagged deep sequencing to quantify HIV-1 splic

149 f 196 isolates of E. coli and K. pneumoniae (PRIMERS II) were examined.

150 In PRIMERS II, the 2 platforms identified susceptibility ag

151 PRIMERS III establishes that RMDs can discriminate betwe

152 othermal DNA amplification, using mismatched primers in conjunction with a two-round enrichment proce

153 We show that Poleta is able to extend RNA primers in the presence of ribonucleotides (rNTPs), and

154 pin structure, on the 5' end of the blocking primer inhibited Pol epsilon from synthesizing DNA up to

155 The goal of this primer is to concisely summarize hepatic functions with

156 Amplification of primers is achieved using an isothermal polymerization r

157 Inspired by this biological adhesive primer, it is shown that a approximately 1 nm thick cate

158 S) of the rRNA gene with fungal specific ITS primers, ITS barcodes were generated for 33 representati

159 iation, elongation, accurate counting of RNA primer length, primer transfer to Polalpha, and concerte

160 This assay involved a primer library which targeted 3071 amplicons from 2078 e

161 ate zeptomolar detection of Dengue consensus primer (limit of detection: 120x10(-21)M) both in contro

162 ver, these protocols require organization of primer locations across numerous 96 well plates and guid

163 rging, barcode demultiplexing, 5' and 3' PCR primer matching, and duplicate reads collapsing.

164 Two samples failed PCR due to primer mismatches; target enrichment uses multiple baits

165 4 books, including The Cigarette Papers and Primer of Biostatistics He is also a member of the Unive

166 nt RNA polymerase that synthesizes short RNA primers of defined size for DNA polymerases.

167 -inflammatory activities, thereby serving as primers of the immune system.

168 I provide a brief primer on ecological computer vision to outline its goal

169 This review attempts to serve as a primer on the topic for the clinical community by provid

170 We present brief primers on phylogenetic signal and the analytical tools

171 p to 2 target-sequence mismatches within the primer or probe binding region.

172 t of the bonding surfaces and application of primers or composite resins that contain special adhesiv

173 by (1) targeting loci with highly degenerate primers or conserved priming sites, (2) increasing PCR t

174 n bias is reduced considerably by degenerate primers or targeting amplicons with conserved priming si

175 to ensure primer uniqueness, avoids placing primers over sites of known variation, and efficiently p

176 ragment from the cyt b gene with a universal primer pair in HRM analyses.

177 regions that cannot be amplified by a single primer pair, a directed graph analysis method is used to

178 lification of multiple targets with a single primer pair, using MLPA probes containing unique barcode

179 lification of multiple targets with a single primer pair.

180 d that ensures the design of target-specific primer pairs for DNA amplification.

181 We amplified eDNA using 12 primer pairs targeting mammals, fish, amphibians, birds,

182 Indel Group in Genomes (IGG) markers are primer pairs that amplify single-locus sequences that di

183 nce was measured using a qPCR array with 363 primer pairs.

184 tranded circular template DNA using specific primer pairs.

185 Using the first 47 primer pools for wet-lab validation, we sequenced 25Kb

186 moalignments - across the genome to identify primers predicted to bind specifically to the target sit

187 llision with a completed Okazaki fragment or primer-primase complexes as the recycling mechanism.

188 T4 bacteriophage DNA replication system that primer-primase complexes have a residence time similar t

189 We examined the role of RNA primer-primase complexes left on the lagging ssDNA from

190 The collision with primer-primase complexes triggering the early terminatio

191 ch can be affected by sequence mismatches in primer/probe binding regions, RT-dPCR may be the optimal

192 s with significant nucleotide mismatching in primer/probe binding regions, while evaluation of a WHO

193 -specific primers and probes and a consensus primer/probe set targeting the 5' noncoding region of HR

194 This primer/probe system did not amplify DNA from 19 other an

195 fferent deer species, a fallow deer-specific primer/probe system targeting a fragment of the nuclear

196 DNA synthesis by DNA polymerase requires RNA primers produced by DNA primase.

197 This primer provides tools and resources for clinicians who w

198 cations such as simplified templates for PCR primers, randomized sequencing and DNA based devices.

199 g LAMP assays based on state-of-the-art, six-primer reactions.

200 loading, mixing, and aliquoting of the LAMP primers/reagents and DNA sample solutions were accomplis

201 mers target a region upstream of the FIP/BIP primer recognition sequences on opposite strands, substa

202 Next, the primers released from above "polymerization-nicking" cyc

203 ucleotides included at the 3'-end of the PCR primers result in additional genome reduction as compare

204 polymerase amplification, exploiting tailed primers, resulting in an amplicon of a duplex flanked by

205 This Primer reviews the mechanisms underlying ferroptosis, hi

206 ssential before nucleophilic attack from the primer RNA 3'-hydroxyl.

207 ommunities and field samples is important in primer selection.

208 Primer sequences flanking polymorphic insertions/deletio

209 f the high copy number feature, a nrdB-based primer set RNRf/RNRr was designed and evaluated using re

210 a previously described and broadly reactive primer set targeting the overlapping open reading frame

211 Thus, despite the addition of a new primer set to an already complex assay, no significant i

212 al-time PCR (RT-PCR) method, employing novel primer sets designed on Jug r 1, Jug r 3, and Jug r 4 al

213 we present swga , a program that identifies primer sets for SWGA and evaluates them for efficiency a

214 We used swga to design and test primer sets for the selective amplification of Wolbachia

215 In addition, we describe characteristics of primer sets that correlate with successful amplification

216 We identify primer sets that successfully amplify each against their

217 To confirm bioinformatic predictions, 384 primer sets were designed along the length of the crAssp

218 ess to evaluate the vast number of potential primer sets.

219 A primer specific region of human mitochondrial D-Loop was

220 Targeting rDNA region nucleotide sequences, primers specific for C. auris only or C. auris and relat

221 in reaction using a quadruple-tagging set of primers specific for E. coli eaeA (151bp) and Salmonella

222 Using primers specific for each viral contig, at least one fun

223 Five primers specific for the N gene sequence of PEDV were us

224 yo-EM analysis reveals the entry path of the primer strand in the PHP-exonuclease active site.

225 enable this amplification: a facilitation of primer strand invasion into double-stranded DNA, and a s

226 Absent Me(2+), the primer strand transfer pathway between the polymerase an

227 of magnitude when compared with conventional primers such as noncatecholic silane- and phosphate-base

228 PriX is essential for primer synthesis and is structurally related to the Fe-S

229 iling divergent models for the regulation of primer synthesis and revealing an underlying plasticity

230 Mn(II) substitution leads to elevated RNA primer synthesis by T7 DNA primase.

231 The intricate mechanism of concerted primer synthesis by two catalytic centers was an enigma

232 n vivo and was dependent on the frequency of primer synthesis downstream of the lesion.

233 ase complexes left on the lagging ssDNA from primer synthesis in initiating early lagging-strand poly

234 tains a nucleotide-binding site required for primer synthesis, and demonstrate equivalence of nucleot

235 al that the Fe-S cluster is not required for primer synthesis.

236 residues that are known to be important for primer synthesis.

237 S cluster domain retains wild-type levels of primer synthesis.

238 The primers target a region upstream of the FIP/BIP primer r

239 sequencing of PCR amplicons generated using primers targeting either ketosynthase domains from polyk

240 Broadly reactive RT-qPCR primers targeting HEV ORF2/3 were successfully adapted f

241 ince this method does not involve degenerate primers targeting HPV genomic regions, PCR bias in genot

242 and tumor DNA with polymerase chain reaction primers targeting RB1 gene c.1075A demonstrated this sam

243 0,000 reactions and amplified with universal primers targeting the bacterial 16S gene.

244 igonucleotide barcodes flanked by sequencing primer targets enables quantitative assessment of the se

245 RET (smFRET) measurements on Cy3/Cy5-labeled primer-template (p/t) DNA constructs in the presence of

246 Clamps must be loaded onto primer-template DNA (ptDNA) by clamp loaders that open a

247 ion by promoting holoenzyme assembly through primer-template docking, accelerate RPR evolution, and a

248 for their ability to unblock and extend DNA primers terminated with AZT and other NRTIs, when comple

249 When the primer terminates across from the adduct position, the a

250 helix has been suggested as the of basis for primer termination in eukaryotes.

251 Extension from DNA damage at primer termini remains poorly understood.

252 er, either result leads to DNA damage at the primer terminus (3-end) during the succeeding insertion

253 el insights into the impact of damage at the primer terminus on genomic stability and DNA synthesis.

254 polymerase beta, accommodates 8-oxoG at the primer terminus opposite cytosine and adenine.

255 When 8-oxoG is at the primer terminus opposite cytosine, DNA centric changes l

256 e intermediate likely still retained the RNA primer that is attached to the 5' end of the plus strand

257 tection, based on inclusion of a dye-labeled primer that is incorporated into a target-specific ampli

258 Using universal primers that amplify the hypervariable bacterial 16 S rR

259 ymerase responsible for synthesis of the RNA primers that are elongated by the replicative DNA polyme

260 h interfacial mussel foot proteins (mfps) as primers that attach to mineral surfaces via hydrogen, me

261 xt-generation sequencing was performed using primers that flanked germline mutations, whose design di

262 nology (LSG-qPCR), which uses genus-specific primers that target the ITS1 region and amplify DNA from

263 As I shall explain in this Primer, the 'ABC model' is a simple and satisfying expla

264 ses TaqMan technology, employing two forward primers, three reverse primers, and four fluorophore-lab

265 nker sequences and modular template-specific primers to allow for the simultaneous generation of high

266 The method utilizes specific primers to amplify target transgenes, and endogenous ref

267 pha (Polalpha), synthesizes chimeric RNA-DNA primers to be extended by replicative DNA polymerases de

268 The results of adding swarm primers to conventional LAMP reactions include increased

269 hancement is achieved by adding a new set of primers to conventional LAMP reactions.

270 chnology combined with Primer ID-tagged cDNA primers to efficiently quantify HIV-1 splicing at a dept

271 s hydrolyzed to 3'-hydroxyl, thus serving as primers to initiate the polymerization extension and nic

272 nd and deliver suitable maltooligosaccharide primers to SS4.

273 ion, accurate counting of RNA primer length, primer transfer to Polalpha, and concerted autoregulatio

274 ch attenuates DNA charge transport, inhibits primer truncation.

275 for speed and uses a hashed genome to ensure primer uniqueness, avoids placing primers over sites of

276 (Lys3) to serve as the reverse transcription primer via an interaction between lysyl-tRNA synthetase

277 For CSE, the self-etching primer was applied and treated with 0.3 M EDC for 1 min,

278 This Primer was developed to provide context for recent studi

279 mplification products based on labeled probe primers was conducted with strip binding antibody of lab

280 In this Primer we will discuss some of the key aspects of signal

281 In this primer, we ask the questions 'What defines a functional

282 In this Primer, we first review the theoretical framework provid

283 In this Primer, we frame the distinctions across the different c

284 In this primer, we outline 6 steps for initiating, implementing,

285 In this Primer, we provide an overview of the Fox family, highli

286 In this Primer, we review the current state of mass cytometry, p

287 In this Primer, we summarize the processes of spermatogenesis oc

288 In this Primer, we will discuss the recent and converging eviden

289 In this Primer, we will probe three axes of invertebrate diversi

290 Using species-specific primers, we found a significant increase in MMP-9 gene e

291 Experimental primers were applied to dentin for 20 s, covered with hy

292 Species-specific primers were designed from cytochrome b, cytochrome oxid

293 For molecular diagnostics, primers were designed from the capsule locus of strain A

294 Six multiplex-compatible PCR primers were designed to distinguish Streptococcus pneum

295 New primers were designed to target the 16S rRNA gene, as a

296 The newly designed primers were evaluated using genomic DNA from 11Ricketts

297 This Primer will focus on several aspects of the remarkable b

298 This method and the compendium of probes and primers will be a useful resource for the plant research

299 e results suggest that the addition of swarm primers will likely benefit most if not all existing LAM

300 with an RNA primer are preserved with a DNA primer--with the same set of polymerase residues trackin