ライフサイエンスコーパス: primer pair

1 wing an initial amplification with the G1-G2 primer pair.

2 ngle preparation of genomic DNA and a single primer pair.

3 d with a consensus and Four Corners-specific primer pair.

4 om this reaction was amplified with a second primer pair.

5 s detected by RT-PCR using a custom-designed primer pair.

6 and replaced with sequences to match any PCR primer pair.

7 lification of multiple targets with a single primer pair.

8 fficient solution-phase PCR using one common primer pair.

9 ine DNA extraction yielded 17 clone-specific primer pairs.

10 llowed multiplexing with several independent primer pairs.

11 ommercially available multiplex set of eight primer pairs.

12 ive PCR (QPCR) analysis with genome-specific primer pairs.

13 ing specific polymerase chain reaction (PCR) primer pairs.

14 re developed independently and had different primer pairs.

15 zed by PCR using a set of approximately 6000 primer pairs.

16 virus was detected by PCR using gag-specific primer pairs.

17 ar process is used to add probes to selected primer pairs.

18 which amplify multiple sequences using short primer pairs.

19 allowing for simplified selection of optimal primer pairs.

20 tranded circular template DNA using specific primer pairs.

21 nce was measured using a qPCR array with 363 primer pairs.

22 ts of these genes, and designs potential PCR primer pairs.

23 lication status of each virus using specific primer pairs.

24 ajor primer design engine to choose the best primer pairs.

25 sitioning only the perfectly matched cognate primer pairs.

26 ted by a multiplex PCR in this case with 170 primer pairs.

27 We found the popular primer pair 515F (515F-C) and 806R greatly underestimate

28 Using these five primer pairs, 54% of the positive saliva samples were de

29 regions that cannot be amplified by a single primer pair, a directed graph analysis method is used to

30 Two primer pairs (actin and ribonucleotide reductase) were c

31 ix molecular beacons and two oligonucleotide primer pairs, allowing for detection and classification

32 The primer pair also amplified an approximately 370-bp fragm

33 The primer pair amplified a single specific product from eac

34 These primer pairs amplified 94 loci, 57 (61%) of which segreg

35 Thirty-two primer pairs amplified identical fragments from all 44 i

36 fragments from all 44 isolates, while eight primer pairs amplified regions that were polymorphic bet

37 However, when primer pairs amplifying the seven polymorphic AvrII site

38 med by RT-quantitative PCR using a set of 42 primer pairs amplifying var subtype-specific loci coveri

39 A large CISP set (6,062 primer pairs, amplifying introns from 1,676 genes) desig

40 isoform mRNA is quantified using a specific primer pair and a 5'FAM- and 3'TAMARA-labeled probe in c

41 itions for use with the previously described primer pair and determined the limit of detection under

42 s encoded by the 16S rRNA gene serves as the primer pair and is used in combination with both an inte

43 PCR with the SM1-SM4 primer pair and isolated genomic DNA as a template resul

44 repeat (SSR) (also known as microsatellite) primer pairs and a plastid DNA deletion marker that dist

45 so carried out regarding the design of NASBA primer pairs and detection probes, as well as reaction a

46 RT-PCR analysis using isoform-specific primer pairs and detector oligonucleotides suggests that

47 e the impact of template type, master mixes, primer pairs and, crucially, extraction methods on dPCR

48 As part of the tiling process, primer pairs are computationally screened to meet the cr

49 tally validated primers, only a few thousand primer pairs are currently present in existing databases

50 Finally, both primer pairs are highly sensitive (>99%) in amplifying v

51 Finally, a set of primer pairs are presented for the amplification of the

52 AMP is challenging because a large number of primer pairs are required to detect alterations in the h

53 Primer pairs are then selected computationally to produc

54 for selective amplification, since only the primer pair associated with each amplification need be u

55 In this way a specific hazelnut primer pair based on the ITS marker (70 bp) and a nuclea

56 s of E. canis were amplified by PCR with two primer pairs based on the sequences of E. chaffeensis om

57 ecanucleotides), and the combined ability of primer pairs, based on these oligonucleotides, to detect

58 molecules with ends complementary to a given primer pair but containing nonhomologous central sequenc

59 It not only designs primer pairs but also finds potential isoforms and homol

60 In this report, a set of six novel primer pair combinations, which can be used with previou

61 In total, 240 primer-pair combinations were used to generate >48,000 i

62 The PGMY L1 consensus primer pair combined with the line blot assay allows the

63 etect three common K-ras codon 12 mutations, primer pairs complementary to the wild-type and mutant l

64 amplification is carried out using a generic primer pair corresponding to the oligonucleotide and gen

65 festans RXLR effector clones, obtained using primer pairs corresponding to 32 genes and assigned acti

66 The DNA was amplified with primer pairs covering hypervariable regions of the 16S r

67 In all, 26,855 primer pairs covering most known mouse genes have been e

68 PrimerBank is the experimental validation of primer pairs covering most known mouse genes.

69 analysis is required in order to screen out primer-pair cross interactions.

70 l (LATE)-PCR, solves these problems by using primer pairs deliberately designed for use at unequal co

71 ative PCR of stool DNA with species-specific primer pairs demonstrated significantly reduced levels o

72 ices for the mock community data for the two primer pairs demonstrated that the V3-V4 hypervariable r

73 Ang-1 was confirmed by RT-PCR using specific primer pairs derived from junction sites and the 3' end

74 Over 100 primer pairs derived from noncoding regions of known gen

75 PCR amplification utilizing primer pairs derived from the 5'-UTR reported herein amp

76 rom virus genome DNA by PCR with a consensus primer pair designed from sequences determined for the c

77 er sets were identified by serial testing of primer pairs designed from KLKB1 cDNA sequence and DNA s

78 ate mRNA from T560 cells and oligonucleotide primer pairs designed from the published sequence of mou

79 hromosomal hybrid DNAs were PCR amplified by primer pairs designed to the 2p11 reference sequence.

80 Some primer pairs designed were able to amplify all splice va

81 s (verifiable by sequencing) with degenerate primer pairs designed within the butyrate kinase gene or

82 Among the 55 primers pairs designed from clones from pathotype 3 of P

83 itivity, compared to an 87% sensitivity with primer pairs directed to EcoRI fragment D, 32% sensitivi

84 Of 148 specimens, primer pairs directed to the HindIII-X fragment region o

85 ed to EcoRI fragment D, 32% sensitivity with primer pairs directed to the immediate-early antigen 1 g

86 1 gene (IEA1 gene), and 20% sensitivity with primer pairs directed to the major immediate-early (MIE)

87 ed and analyzed by PCRs with three different primer pairs directed toward bacterial rRNA loci.

88 eviously, we demonstrated the utility of two primer pairs, directed to the rRNA operon, to specifical

89 Overall, this primer pair displayed a sensitivity and specificity of 9

90 For two of these ESTs we tested fourteen primer pairs each and using standard optimization method

91 rect assay that amplifies the 16S rRNA gene (primer pair ehr521-ehr790; detects 0.25 infected cell).

92 Each primer pair either specifically amplified a DNA fragment

93 ave an option to add a probe to any selected primer pair, enabling design of Taqman and other primer-

94 been designed and in many cases alternative primer pairs exist.

95 Most primer pairs facing in opposite directions gave several

96 the ADH1 and MATalpha loci by PCR, using the primer pairs flanking the Ho cut sites.

97 silosis sensu lato FKS1 gene and a universal primer pair followed by EcoRI enzyme digestion.

98 a lower annealing temperature or a different primer pair for the HIV-1 pol region.

99 Primer pairs for 314 SSLP markers were designed and type

100 We list primer pairs for amplifying each exon, with flanking int

101 d that ensures the design of target-specific primer pairs for DNA amplification.

102 esource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Qua

103 n software application that designs multiple primer pairs for generating PCR probes useful for fluore

104 e fragments to the Primer3 program to design primer pairs for PCR amplification.

105 xperimentally validated collection of murine primer pairs for PCR and QPCR which can be used under a

106 e designed a "one-tube test" comprising four primer pairs for polymorphic tetranucleotide repeat sequ

107 pairs for sequences on chromosome 18, three primer pairs for sequences on chromosome 13, and one pri

108 tide repeat sequences on chromosome 21, four primer pairs for sequences on chromosome 18, three prime

109 ies; these regions were used to design three primer pairs for species-specific PCR.

110 Intron sequences were used to design primer pairs for the amplification of all exons.

111 Primer pairs for the amplification of each of the 13 p73

112 thms designed to select and match degenerate primer pairs for the amplification of viral genomes.

113 was to compare published Helicobacter pylori primer pairs for their ability to reliably detect H. pyl

114 es were used to design 13 taxon-specific PCR primer pairs for those taxa most commonly implicated in

115 ere reproducibly obtained for seven of eight primer pairs from chromosomal DNA of 33 of the 35 isolat

116 One of these primer pairs, G1-G2, only amplified a DNA fragment from

117 sted assay that amplifies the 16S rRNA gene (primer pairs ge3a-ge10 [primary] and ge9-ge3 [nested]; d

118 both populations using previously published primer pairs generated sequences containing multiple sub

119 RNA clone libraries generated with different primer pairs, > 99% and 100% of the sequences were affil

120 For each gene, at least one primer pair has been designed and in many cases alternat

121 The primer pairs have been validated by gel electrophoresis,

122 amplified fragments (AFs) generated with the primer pair HindIII+A and HhaI+A were compared.

123 Five of the twenty-six previously reported primer pairs (HP64-f/HP64-r, HP1/HP2, EHC-U/EHC-L, VAG-F

124 he MY09-MY11 and the GP5(+)-GP6(+) consensus primer pairs, HPV sequences were amplified by nested PCR

125 No combinations of primer pairs improved the results.

126 ragment from the cyt b gene with a universal primer pair in HRM analyses.

127 The presence of a single universal PCR primer pair in solution accelerates amplification within

128 lomerase bypasses a requirement for template-primer pairing in order to add telomeric DNA de novo to

129 re data generated from two platforms for two primer pairs in a low density mock bacterial community.

130 In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation i

131 cpz and HIV-1 lineages (groups M, N, and O), primer pairs in conserved pol and env regions were desig

132 n locus (cps) of vaccine serotypes to locate primer pairs in conserved regions that border variable r

133 The assay panel included 16 primer pairs in eight multiplexed reactions and was vali

134 n polymorphism and sequencing with arbitrary primer pairs in molecular population genetics.

135 However, use of a pol primer pair increased the sensitivity of the assay to 98

136 96 clones) using a third universal bacterial primer pair (inosine-341f and 1492r).

137 Primer pairs (intHIV-2/SIV) based on a region of integra

138 sequence-based method employing a single PCR primer pair is cost-effective and simple.

139 The complex primer pair is covalently tethered through its 5' end to

140 A variant-specific primer pair is required to amplify each alternatively sp

141 However, designing subtype-specific PCR primer pairs is a very challenging task: on one hand, se

142 PCR with single primer IS3A and primer pair IS3A-IS3B identified 6 and 14 different subt

143 Amplification of adjacent primer pairs, labeled in two colors, allowed us to narro

144 orghum RIL mapping population using the same primer pairs located approximately 200 of the BAC contig

145 e chain reaction (RT-PCR) was performed with primer pairs located in exons flanking the exon (extra d

146 iii) an M. catarrhalis-specific primer pair (primer pair MCA1-MCA2).

147 plifies the major surface protein gene msp2 (primer pair msp2-3f-msp2-3r; detects 0.25 infected cell)

148 very challenging task: on one hand, selected primer pairs must result in robust amplification in the

149 We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-sub

150 sequenced and then used to design a specific primer pair, Nf1 (16-mer) and Nf2 (16-mer), complementar

151 However, real-time PCR using gene-specific primer pairs only amplified Ee-BAM1, indicating that Ee-

152 reaction with genomic DNA, single primers or primer pairs oriented in the same direction showed negli

153 PsASGR-BABY BOOM-like (psASGR-BBML)-specific primer pair p779/p780 was in perfect linkage with the AS

154 The SlipChip was designed to preload one primer pair per reaction compartment and to screen up to

155 Detection limits of the 26 PCR primer pairs previously described for detection of H. py

156 h DG74, and (iii) an M. catarrhalis-specific primer pair (primer pair MCA1-MCA2).

157 Redesign of six of the primer pairs (Primer set_B), re-genotyping of the succes

158 gments were amplified with several different primer pairs prior to cloning and sequencing or communit

159 The new primer pair/probe set detected 140 of 141 isolates, incl

160 ersion of the AMPLICOR HIV-1 test, and a new primer pair/probe system.

161 In addition, the 16S primer pair putatively identified B. cepacia in seven pa

162 btained different statistical outcomes among primer pairs, raising unexpected but serious questions a

163 Minimally, testing numerous primer pairs seems to be necessary to avoid false conclu

164 se chain reaction (RT-PCR) using a published primer pair sequence followed by restriction endonucleas

165 ts, we designed 410 evolutionarily conserved primer pair sequences which are specific for anchor locu

166 Two of the primer pairs showed 100% sensitivity and specificity for

167 tent with the primer extension results, some primer pairs showed no amplification, indicating that th

168 Amplification with the same primer pairs showed strong concordance within each platf

169 Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in di

170 reparation, PCR-based amplification with the primer pair SK39 (biotinylated at the 5' end) and SK38,

171 is of cps from 92 serotypes indicated that a primer pair spanning the regulatory gene cpsB could puta

172 ite polymerase chain reaction (PCR) using 15 primer pairs spanning the common deleted region on 20q s

173 rate PCR reactions, each of which contains a primer pair specific to one or the other allelic SNP var

174 amplified by polymerase chain reaction using primer pairs specific for the DQB loci followed by South

175 he HLA DR loci (n = 1) or inability to obain primer pair specificity among similar HLA DR types (n =

176 Successful cross-amplification for 12 primer pairs studied in detail using DNA from downy mild

177 h multiplex PCR derived from the use of many primer pairs such as false amplicons, primer-dimers etc.

178 re evaluated with a previously described gag primer pair system.

179 The design of panels of hundreds of primer pairs takes just hours using this program, compar

180 n for all the genomovars indicated that this primer pair targeted a region shared by these isolates.

181 a given environment by amplifying eDNA using primer pairs targeting a wide range of taxa and high-thr

182 We amplified eDNA using 12 primer pairs targeting mammals, fish, amphibians, birds,

183 Six mini-barcode primer pairs targeting short (127-314 bp) fragments of t

184 We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that

185 Analysis by the PCR/ESI-MS method using nine primer pairs targeting the most information-rich regions

186 considered a common feature of PCR, 30 of 32 primer pairs tested in this study produced primer-dimer

187 le product), determined that of the fourteen primer pairs tested, six and nine pairs respectively amp

188 Here we present a primer pair that eliminates this problem, allowing simpl

189 The conclusions were validated by using primer pairs that amplify sequences within the cystic fi

190 Indel Group in Genomes (IGG) markers are primer pairs that amplify single-locus sequences that di

191 The assay utilizes 24 primer pairs that amplify targets in the mtDNA control r

192 Ten primer pairs that anneal upstream and downstream of each

193 endonuclease restriction maps, we chose PCR primer pairs that approximated and framed junctions crea

194 ate the list of candidate primers, to choose primer pairs that are best suited for their needs.

195 regions to allow design of a limited set of primer pairs that can selectively target coding regions

196 In this work, we identified four PCR primer pairs that collectively amplified cdt genes in al

197 mark PCR Arrays from SuperArray, containing primer pairs that have been experimentally validated to

198 and conformation polymorphism analysis using primer pairs that span the BRCA1 coding region and intro

199 RT-PCR using primer pairs that were designed to detect specifically n

200 Using two primer pairs that will either specifically amplify the l

201 r binding regions used for the MY09 and MY11 primer pair, this PCR assay detects the presence of 27 H

202 t from thousands of computationally proposed primer pairs those that are specific to individual rdh g

203 Using a specific primer pair to amplify Nanog by reverse transcriptase-PC

204 airs for sequences on chromosome 13, and one primer pair to identify the sex chromosomes.

205 e chain reaction assay that employs the same primer pair to simultaneously amplify ERalpha and ERbeta

206 quential amplification reactions with nested primer pairs to achieve the necessary specificity and se

207 We designed PCR primer pairs to amplify genomic DNA flanking each of 40

208 region of the mutation by using intron/exon primer pairs to amplify regions of the precursor nuclear

209 med tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different

210 onstrate this principle, we designed 384 PCR primer pairs to conserved exonic regions flanking intron

211 d on the 384-well SlipChip with 20 different primer pairs to identify 16 bacterial and fungal species

212 LST housekeeping gene loci by using the same primer pairs used in MLST.

213 ber of different bands detected for each SSR primer pair using a LI-COR- DNA Analyzer ranged from two

214 lification of multiple targets with a single primer pair, using MLPA probes containing unique barcode

215 tions than does another previously described primer pair, V1/EB450.

216 Two primer pairs (W3F-W4R, W3F-W2R) were used in separate po

217 coding success rate found with an individual primer pair was 88.6% compared to 20.5% success rate ach

218 An outer primer pair was designed to amplify an approximately 340

219 previous studies in which only a single PCR primer pair was used may have significantly underestimat

220 A novel PCR primer pair was used to detect the presence of cmeC in 1

221 One of the primer pairs was further assessed for species specificit

222 nformation was unavailable, selection of PCR primer pairs was guided by general insights gleaned from

223 Using a single primer pair, we generated amplicons of high sequence com

224 lymorphism fingerprinting technique with two primer pairs, we identified 12 genotypes among the isola

225 0 degrees C annealing temperature or the pol primer pair were 71 and 98%, respectively.

226 A set of four species-specific real-time PCR primer pairs were also designed, capable of detecting le

227 Multiple primer pairs were designed from the sequence of a 200 kb

228 ping among legumes; thus 60 of the EST-based primer pairs were designed to amplify orthologous sequen

229 Primer pairs were designed to span five different nucleo

230 Primer pairs were designed to span introns for polymorph

231 Further, virus-specific primer pairs were identified for California encephalitis

232 A total of 318 primer pairs were optimized for domestic cats, and 86% o

233 Two primer pairs were putatively specific for B. cepacia.

234 The primer pairs were separated and collected by IP-RP HPLC.

235 of quantitation using PCR utilizing dual LUX primer pairs were similar to quantitation using single L

236 A total of 187 primer pairs were tested under five different abiotic st

237 These primer pairs were tested with 44 O157 isolates, 2 each f

238 The mini-barcode primer pairs were then tested against 44 processed fish

239 Nine primer pairs were used to generate AFLP patterns, with a

240 Degenerative primer-pairs were first designed based on the highly con

241 confound standard-design "RNA-specific" PCR primer pairs which rely, for cDNA versus gDNA specificit

242 Here, seven primer pairs (which amplify within the loci 16S rRNA, Rv

243 f this product was used to design a specific primer pair, which generated a single 864-bp fragment wi

244 tional and real-time PCR for a subset of 112 primer pairs with a success rate of 98.2%.

245 temperature for each primer and selects the primer pairs with comparable annealing temperature.

246 dal B cell lymphomas have employed different primer pairs with detection sensitivities varying betwee

247 A number of different primer pairs with homology to most known genital HPV typ

248 ompartment and to screen up to 384 different primer pairs with less than 30 nanoliters of sample per

249 e RNAs, provided that the 3' terminus of the primer pairs with the template.

250 es, or anchored PCR, using C region-specific primers paired with vector specific primers.

251 Primer pairs within the 3' untranslated region of PCTA-1