ライフサイエンスコーパス: primitive streak

1 elop beyond 14 days or the appearance of the primitive streak.

2 ectively, inhibit or induce formation of the primitive streak.

3 rsors located in the epiblast lateral to the primitive streak.

4 in E-cadherin protein downregulation in the primitive streak.

5 necessary for migration of mesoderm from the primitive streak.

6 e epiblast resulting in the formation of the primitive streak.

7 however, were those expressed in vivo in the primitive streak.

8 ls at stages leading to the formation of the primitive streak.

9 rm, a derivative of the middle region of the primitive streak.

10 or the proper morphogenesis of the mammalian primitive streak.

11 n the limb bud as well as axial mesoderm and primitive streak.

12 er maintained at a defined distance from the primitive streak.

13 y as a mesodermal extension of the posterior primitive streak.

14 ntains the remnants of Hensen's node and the primitive streak.

15 ent as they underwent ingression through the primitive streak.

16 ctivated but is faithfully maintained in the primitive streak.

17 senchymal transition and ingress through the primitive streak.

18 ists between the left and right sides of the primitive streak.

19 ial for the normal formation of the node and primitive streak.

20 tial for HNF3beta expression in the anterior primitive streak.

21 hric or mesonephric precursor cells from the primitive streak.

22 l array extending the complete length of the primitive streak.

23 istance from the organizer at the tip of the primitive streak.

24 the trunk organizer in the node and anterior primitive streak.

25 mesodermal cells are not recruited into the primitive streak.

26 distant from the signal, to form an ectopic primitive streak.

27 1 and Wnt8C, is located in the middle of the primitive streak.

28 ectoderm is dependent on derivatives of the primitive streak.

29 is required for cell migration away from the primitive streak.

30 ), prior to formation of the fully elongated primitive streak.

31 subsets, although it is not detected in the primitive streak.

32 et of gastrulation with the formation of the primitive streak.

33 population found at the anterior end of the primitive streak.

34 he anterior visceral endoderm and the distal primitive streak.

35 ption of signalling activity in the anterior primitive streak.

36 oderm isolates lacking Hensen's node and the primitive streak.

37 o occurs in vivo as epiblast cells enter the primitive streak.

38 sed signalling cues from areas including the primitive streak.

39 sendoderm, which is a major component of the primitive streak.

40 vely uncoupling it from the induction of the primitive streak.

41 ally deleted from the migrating mesoderm and primitive streak.

42 mesoderm precursors to migrate away from the primitive streak.

43 he embryo and a gain of Wnt signaling in the primitive streak.

44 a specific progenitor population in the late primitive streak.

45 ix adhesion or by signals from the posterior primitive streak.

46 al-to-mesenchymal transition at the anterior primitive streak.

47 to disrupted germ-layer morphogenesis at the primitive streak.

48 ientation of mesoderm cells as they exit the primitive streak.

49 ts are inhibited and cells accumulate in the primitive streak.

50 lecules BMP4 and Vg1/GDF1 in positioning the primitive streak.

51 erm cells after their ingression through the primitive streak.

52 abrogates normal cell migration through the primitive streak.

53 By stage 6, shortly after cells leave the primitive streak, a field of cells is generate which is

54 er and from other more caudal regions of the primitive streak act on the rostral prospective neuroect

55 to the normal derivatives of the definitive primitive streak along its rostrocaudal extent and which

56 monstrated that Alk2 mutant embryos formed a primitive streak, although abnormally thickened, and wer

57 ouble homozygous mutants fail to establish a primitive streak, although the anterior visceral endoder

58 and exhibit an accumulation of cells at the primitive streak and a selective loss of paraxial mesode

59 g between extrinsic mechanisms involving the primitive streak and an intrinsic role for the allantois

60 rovide evidence that Wnt3a, expressed in the primitive streak and dorsal posterior node, acts as a lo

61 pression is first detected at stage 3 in the primitive streak and exhibits transient low-level expres

62 ing event that leads to the formation of the primitive streak and gastrulation.

63 nd foregut lining, emerges from the anterior primitive streak and Hensen's node as a cell monolayer t

64 E-cadherin needs to be downregulated in the primitive streak and in the epiblast, concomitant with t

65 The establishment of the primitive streak and its derivative germ layers, mesoder

66 tion of progenitors initially located in the primitive streak and later in the tail bud.

67 he epiblast is required for induction of the primitive streak and mesoderm whereas activity in the po

68 ed visceral endoderm and subsequently to the primitive streak and mesoderm.

69 ting that loss of pluripotency, formation of primitive streak and mesodermal lineage progression are

70 gene family that is highly expressed in the primitive streak and migrating mesoderm cells on embryon

71 Cre identifies a requirement for Zic3 in the primitive streak and migrating mesoderm for proper left-

72 However, the defects in both the lulu primitive streak and neural plate are associated with di

73 iblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm.

74 The primitive streak and node become disfigured, consistent

75 on of Wnt-3a and Wnt-8c is restricted to the primitive streak and posterior lateral plate, and is abs

76 In the mouse, Tbx6 is expressed in the primitive streak and presomitic mesoderm, and is sharply

77 embryonic polarity: it helps to position the primitive streak and some have suggested that it might a

78 ryonic day 7 (E7) in the neural ectoderm and primitive streak and subsequently in the brain, peripher

79 use embryogenesis, Gdf11 is expressed in the primitive streak and tail bud regions, which are sites w

80 of resident cells located in the regressing primitive streak and tail bud.

81 bilaterally to the nerve cord as a result of primitive streak and tail-bud regression during body axi

82 uction pathways that direct cell fate in the primitive streak and tailbud of the early embryo.

83 sis and in the production of tissue from the primitive streak and tailbud.

84 Zeb2 repress P-cadherin transcription in the primitive streak and the neural plate, respectively.

85 ontaining cells fated to ingress through the primitive streak and to form lateral plate mesoderm; and

86 ion system to examine formation of the chick primitive streak and to test the proposal that oriented

87 he caudal presomitic mesoderm (PSM) from the primitive streak (and later the tail bud), while somites

88 , shortly after its gastrulation through the primitive streak, and earlier than previously reported.

89 l of individual cells in the mouse epiblast, primitive streak, and early YS.

90 s expressed in inner cell mass cells and the primitive streak, and later is restricted to the develop

91 , Cr-1 is expressed in the mouse blastocyst, primitive streak, and later is restricted to the develop

92 tributes prospective endodermal cells to the primitive streak, and subsequently to definitive endoder

93 ibutions mainly from the rostral half of the primitive streak, and that endodermal movements parallel

94 al end of the embryo, whereas markers of the primitive streak are absent or localized to the proximal

95 migration of nascent mesodermal cells in the primitive streak as the morphogenetic basis underlying t

96 To gain insight into primitive streak assembly and function, we have conducte

97 Impaired primitive streak assembly in the mouse amnionless (amn)

98 y in size after E7.0, indicating that middle primitive streak assembly is mechanistically tied to the

99 We propose that these cells arise within primitive streak-associated epiblast via a mechanism tha

100 ough required, does not autonomously promote primitive streak-associated gene expression in vitro.

101 of Wnt activity promotes restriction towards primitive streak-associated lineages with mesendodermal

102 ome cells in lulu mutants are trapped in the primitive streak at an intermediate stage of the epithel

103 of Pten null embryos express markers of the primitive streak at ectopic locations around the embryon

104 The mouse posterior primitive streak at neural plate/headfold stages (NP/HF,

105 tly of mesendoderm formation to position the primitive streak at the midline.

106 , arose from the production of more than one primitive streak at the time of gastrulation.

107 Importantly, the node and primitive streak at these stages lack Cx43 mRNA.

108 Epiblast cells adjacent to the regressing primitive streak behave as a stem zone that progressivel

109 s ingressing through the anterior and middle primitive streak, but it does not affect EMT of cells lo

110 th the replacement of the regressed node and primitive streak by a caudal blastema-like mass of mesen

111 at Dact1 contributes to morphogenesis at the primitive streak by regulating Vangl2 upstream of cell a

112 eriments in the mouse have revealed that the primitive streak can be divided into three functional re

113 As reported previously, an ectopic primitive streak can be induced by misexpression of Vg1

114 d/4) that lack Hensen's node (organizer) and primitive streak can reconstitute a functional organizer

115 ssion, which leads to Fgf4 expression in the primitive streak, can also promote mesoderm migration in

116 ls into mesodermal subtypes that reflect mid-primitive-streak cardiogenic mesoderm and posterior-prim

117 e fate, acquiring two distinct properties of primitive streak cells, defined by gene expression and c

118 erm was defined by following labeled rostral primitive-streak cells over a short period of time as th

119 ow that cells that contribute to the ectopic primitive streak change fate, acquiring two distinct pro

120 The acquisition of primitive streak characteristics by self-renewing EpiSCs

121 r subfraction of cells with reversible early primitive streak characteristics, which is mutually excl

122 gesting correspondence to gastrulation-stage primitive streak, chorion and allantois precursors, resp

123 orcine PGCs originate from the posterior pre-primitive-streak competent epiblast by sequential upregu

124 d prospective mesoderm from one level of the primitive streak, containing cells fated to continue ing

125 epithelial to mesenchymal transition in the primitive streak, correct patterning of mesoderm, and lo

126 hird signal, FGF4, which is expressed in the primitive streak, defines an inductive center for hindbr

127 Formation of the node, at the anterior primitive streak, depends on expression of the transcrip

128 mbryos also display an expansion of anterior primitive streak derivatives and anterior neurectoderm t

129 ng allantois, a rudimentary heart and middle primitive streak derivatives such as somites and lateral

130 sults indicate that Lim1 is required in both primitive streak-derived tissues and visceral endoderm f

131 aembryonic anterior visceral endoderm and in primitive streak-derived tissues of early mouse embryos.

132 initiates a process that resembles posterior primitive streak development in a SNAI1-dependent manner

133 lance between BMP and Nodal signaling during primitive streak development, and provide a potential me

134 trulation, when cells ingressing through the primitive streak differentiate into the precursor cells

135 irects FAST site-dependent expression in the primitive streak during gastrulation, and unilateral exp

136 so plays a role in cell movement through the primitive streak during gastrulation.

137 delay and an accumulation of mesoderm in the primitive streak during gastrulation.

138 long-range migration after emerging from the primitive streak during gastrulation.

139 sors called hemangioblasts, specified in the primitive streak during gastrulation.

140 cells in various rostrocaudal levels of the primitive streak during key periods in early development

141 As the primitive streak elongates, this tissue with organizing

142 hat oriented cell division could account for primitive streak elongation.

143 In the primitive streak embryo, GATA-5 mRNA is detectable in th

144 We propose that the amniote primitive streak evolved from the ancestral blastopore b

145 xed, and blood island precursor cells in the primitive streak express many of the same molecular mark

146 ring gastrulation, FOXF1 marks all posterior primitive streak extra-embryonic mesoderm derivatives wi

147 he mouse embryo before the appearance of the primitive streak; first in the posterior visceral endode

148 Smad2 signals serve to restrict the site of primitive streak formation and establish anterior-poster

149 mouse embryos serve to restrict the site of primitive streak formation and establish anteroposterior

150 Gata2 influences the site of primitive streak formation and its role is independent f

151 red for anterior-posterior (A-P) patterning, primitive streak formation and left-right (L-R) axis det

152 (mesd) functional interval is essential for primitive streak formation and mesoderm induction.

153 4 (BMP-4) and a BMP antagonist, chordin, in primitive streak formation and neural induction in amnio

154 of BMP signalling by chordin plays a role in primitive streak formation and that chordin is not suffi

155 tigate the signalling pathways that initiate primitive streak formation and the mechanisms that ensur

156 nce of the marginal zone to initiate ectopic primitive streak formation in response to cVg1 is depend

157 isexpressed in the posterior area pellucida, primitive streak formation is inhibited.

158 In the mouse, primitive streak formation is the first overt morphologi

159 Subsequently, just prior to the time of primitive streak formation, a conformational change in t

160 eptors are required for egg cylinder growth, primitive streak formation, and rostral head development

161 both BMP-4 and chordin are expressed before primitive streak formation, and that BMP-4 expression is

162 g of the visceral endoderm is independent of primitive streak formation, but the subsequent establish

163 controls epiblast cell movements leading to primitive streak formation, generating bilateral symmetr

164 Primitive streak formation, however, was impaired in chi

165 lso show that FGF signalling is required for primitive streak formation, in cooperation with Nodal an

166 function in the posterior epiblast promotes primitive streak formation, whereas transient nodal expr

167 extinguished in the epiblast at the onset of primitive streak formation.

168 spatially directed cytokineses during early primitive streak formation.

169 ited polarized cell divisions during ectopic primitive streak formation.

170 the presumptive neural plate before or after primitive streak formation.

171 nalysis of distinct events that occur during primitive streak formation.

172 r region of the epiblast marking the site of primitive streak formation.

173 The primitive streak forms on the opposite side of the egg c

174 in the epiblast is quite uniform before the primitive streak forms then decreases in the central epi

175 analyses, suggested a defect in the anterior primitive streak, from which much of the embryonic mesod

176 e early gastrula they are located in the mid-primitive streak, from which they enter the mesoderm bil

177 When the primitive streak has reached its full length, a later gr

178 us origins and developmental pathways of the primitive streak have been proposed.

179 ve-streak cardiogenic mesoderm and posterior-primitive-streak hemogenic mesoderm.

180 Chicken Wnt11b is expressed in the primitive streak in a pattern similar to chicken Wnt5a a

181 Initiation of the primitive streak in avian embryos provides a well-studie

182 area pellucida enables this region to form a primitive streak in response to cVg1.

183 tissue flow underlying the formation of the primitive streak in the chick embryo.

184 ion of mesoderm cells ingressing through the primitive streak in the chick embryo.

185 ive neuroectoderm immediately rostral to the primitive streak in the early gastrula.

186 n and avian embryos is the appearance of the primitive streak in the future posterior region of a rad

187 and ingression of mesoderm cells through the primitive streak, including fibroblast growth factors an

188 on of mesenchymal cells around the shortened primitive streak indicating a functional requirement of

189 est frequency in the posterior region of the primitive streak, indicating that initial stages of haem

190 Wnt antagonists Crescent and Dkk-1 block the primitive streak-inducing ability of cVg1 in the margina

191 During mammalian embryonic development, the primitive streak initiates the differentiation of plurip

192 hat Pten is not required in the cells of the primitive streak; instead, Pten is required for normal m

193 First, they emerge from the primitive streak into the region of the endoderm that fo

194 mechanisms that mediate the assembly of the primitive streak into these functional regions, we have

195 ve proximal ACD and intraembryonic posterior primitive streak (IPS) contributed to a wide range of so

196 f prospective paraxial mesoderm cells to the primitive streak is delayed.

197 8 expression in nascent mesoderm exiting the primitive streak is dramatically reduced in mutant embry

198 k stages XI-XII through HH stage 3, when the primitive streak is initially established and prior to t

199 in other vertebrates as it suggests that the primitive streak is initially established in a radial pa

200 The primitive streak is the defining feature of the gastrula

201 In the chick embryo, the primitive streak is the first axial structure to develop

202 y is required for cell migration through the primitive streak, is a target of miR-130b and -218 in vi

203 ior epiblast, and hence the formation of the primitive streak, is the result of local cell-cell inter

204 a late inhibition from Lefty emitted by the primitive streak itself.

205 astrulation, ingressing through a "bilateral primitive streak" just inside the blastopore.

206 rcation event early in the trajectory when a primitive streak-like cell population segregated into th

207 says and embryo grafting we demonstrate that primitive streak-like EpiSCs are biased towards mesoderm

208 Generation of a primitive streak-like population in embryoid bodies, fol

209 ation, Rac1Deltaepi embryos have an enlarged primitive streak, make only a small amount of paraxial m

210 eak border) in which ectoderm that expresses primitive streak markers overlies the prospective notoch

211 to a linear transition in the expression of primitive streak markers precedes the formation of the p

212 gulates its own expression and that of other primitive streak markers via activation of the canonical

213 ferentiation, accelerating the expression of primitive streak markers.

214 circle and a ring of mesendoderm expressing primitive-streak markers in between.

215 The primitive streak marks the midline of the future embryo.

216 hematopoietic progenitors without affecting primitive streak mesoderm formation.

217 Cell migration, which is characteristic of primitive streak mesoderm in vivo, was not induced by FG

218 ting cells in both the progress zone and the primitive streak mesoderm suggests that one function of

219 for FGF signaling in the induction of mouse primitive streak mesoderm, as well as in later patternin

220 e progressive loss of paraxial, lateral, and primitive streak mesoderm.

221 ranscription factor that is expressed in the primitive streak, mesoderm and anterior mesendoderm of t

222 elop a normal egg cylinder but do not form a primitive streak, mesoderm or node.

223 Furthermore, we show that FGF4 expressed in primitive streak-mesoderm can induce the differentiation

224 somite progenitor cells ingress through the primitive streak, move laterally to a paraxial position

225 at E7.5 with defects in the formation of the primitive streak, node, and mesoderm.

226 crease in canonical Wnt activity in the late primitive streak of all Axin2(canp) mutant embryos that

227 to the chick, as inhibition of K(ATP) in the primitive streak of chick embryos randomizes the express

228 day 8.5 (E8.5) embryos and the head fold and primitive streak of E7.5 embryos.

229 Brachyury mRNA expression in the primitive streak of RIalpha mutants is significantly red

230 3a encodes a signal that is expressed in the primitive streak of the gastrulating mouse embryo and is

231 F family members that are coexpressed in the primitive streak of the gastrulating mouse embryo.

232 streak markers precedes the formation of the primitive streak on one side of the epiblast.

233 mechanisms that mediate the assembly of the primitive streak or about the signaling pathways that sp

234 rally believed to initiate expression at the primitive streak or early neural plate stages.

235 sis suggests that the hESCs progress through primitive streak or mesendoderm to mesoderm, before diff

236 imitive endodermal cell layers, but lacked a primitive streak or recognizable mesoderm.

237 ay 7 of gestation, fail to develop mesoderm, primitive streak, or proamniotic cavity.

238 First, we determined the primitive-streak origin of the endoderm using supravital

239 leads to gastrulation phenotypes, including primitive streak patterning defects in association with

240 or low levels of activin-induced a posterior primitive streak population, whereas high levels of acti

241 with high levels of Gdf11 expression in the primitive streak, presomitic mesoderm, and tail bud.

242 cs, BMP and Wnt initially specified anterior primitive streak (progenitor to endoderm), yet, 24 hr la

243 ion in the anterior visceral endoderm (AVE), primitive streak (PS) and definitive endoderm (DE) have

244 Mesoderm is induced at the primitive streak (PS) and patterns subsequently into mes

245 Abrogation of Smad2 does not compromise primitive streak (PS) formation or gastrulation movement

246 itor based on the temporal expression of the primitive streak (PS) marker brachyury and Flk-1.

247 NMPs are known to reside in the anterior primitive streak (PS) region; however, the extent to whi

248 ells that arise from the anterior end of the primitive streak (PS) to migrate to their correct locati

249 epiblast ingress through a furrow called the primitive streak (PS), and subsequently move away from t

250 tically alters anteroposterior patterning of primitive streak (PS)-like priming.

251 tion, which is initiated by formation of the primitive streak (PS).

252 orphologically stable midline structure, the primitive streak (PS).

253 In mammals, the primitive streak region and its descendant, the tail bud

254 formation and/or specification of the middle primitive streak region during gastrulation.

255 ntify 44 microRNAs that are expressed in the primitive streak region of gastrula stage chicken embryo

256 strulation in the Hoxb1-expressing posterior primitive streak region: Hoxb chromatin was decondensed

257 e mechanisms responsible for positioning the primitive streak remain largely unknown.

258 of stage 4 or 5 quail node into caudal chick primitive streak resulted in the generation of ectopic s

259 with paraxial mesoderm character within the primitive streak, resulting in a lateral expansion of so

260 IIA(-/-) embryos failed to form an elongated primitive streak, resulting in severe disruption of meso

261 a transcription factor required for anterior primitive streak specification during early development,

262 ximal regions of the egg cylinder at the mid-primitive streak stage (E7.0) with the simultaneous appe

263 more, we show that up to and through the mid-primitive streak stage (i.e., stage 3c/3+), the prospect

264 (i.e., is specified) by the fully elongated primitive streak stage (i.e., stage 3d).

265 lation but are unable to progress beyond the primitive streak stage and die shortly afterward.

266 o its highly localized expression during the primitive streak stage and in the adult stage, CAT3 expr

267 d-mutant mouse nodes by transplantation into primitive streak stage chick embryos.

268 remarkable capacity to regulate: when a pre-primitive streak stage embryo is cut into fragments, eac

269 Cultured primitive streak stage epiblast cells downregulate E-cad

270 In contrast to the primitive streak stage epiblast cells, prestreak epiblas

271 The anterior mesendoderm of mid- to late primitive streak stage mouse embryos has the ability to

272 um proteins were synthesized as early as the primitive streak stage.

273 ells at stage 4+, immediately after the full primitive streak stage.

274 revealed the onset of expression in advanced primitive streak-stage embryos being located in the extr

275 to initiate gastrulation and advance to late primitive streak stages but fail to thrive and are resor

276 n is weakly expressed in the epiblast at pre-primitive streak stages where it is substituted for by P

277 ilure to pattern derivatives of the anterior primitive streak, such as prechordal plate, node, notoch

278 ites derives from stem cells resident in the primitive streak/tail bud, the lateral part derives from

279 n normal spatial domains for Cdx expression (primitive streak/tailbud), yet, overall, they contain el

280 otes the expression of genes in the anterior primitive streak that are important for the development

281 anterior area pellucida generates an ectopic primitive streak that expresses mesoderm and organizer m

282 Formation of the primitive streak, the equivalent of the blastopore, is a

283 curs through an axial midline structure, the primitive streak, the formation of which is preceded by

284 lthough Wnt5a and Wnt5b are expressed in the primitive streak, the known Wnt11 gene is expressed in p

285 osterior of GCNF(-/-) embryos, including the primitive streak, the node, and the presomitic mesoderm.

286 While tissue is still in the primitive streak, the prospective IM is relatively uncom

287 ll elongation and alignment upon leaving the primitive streak, the subsequent polarized protrusive ac

288 n the amniote begins with the formation of a primitive streak through which precursors of definitive

289 hordoneural hinge were grafted to 8.5 d.p.c. primitive streak to compare their developmental potency.

290 During mouse gastrulation, cells in the primitive streak undergo epithelial-mesenchymal transfor

291 when transplanted to either the epiblast or primitive streak, undergoing an epithelial-mesenchymal t

292 No significant contribution to the early primitive streak was seen from the anterolateral epiblas

293 f Fgf8 and study its role in lineages of the primitive streak, we have used a new mouse line, T-Cre,

294 dye labeling, precursor cells of the stage 3 primitive streak were mapped predominantly to a specific

295 is required for cell migration away from the primitive streak when gastrulation initiates, but previo

296 n Dact1 mutants, Vangl2 was increased at the primitive streak, where cells ordinarily undergo an epit

297 ion displays characteristics of the anterior primitive streak, whereas the CD4-Foxa2(lo)GFP-Bry(+) ce

298 descendants of 8.5 d.p.c. node and anterior primitive streak which remain in the tail bud are locate

299 al edge of the isolate forms a reconstituted primitive streak, which gives rise to the normal derivat

300 can reconstitute a functional organizer and primitive streak within 10-12 hours in culture.