ライフサイエンスコーパス: proMMP-2

1 ss fibrillar collagen type I and to activate proMMP-2.

2 the cleavage of the N-terminal propeptide of proMMP-2.

3 We have previously shown that LPA promotes proMMP-2 activation and MMP-2-dependent migration and in

4 pon blocking ERK1/2 phosphorylation promoted proMMP-2 activation and MT1-MMP activity, whereas inhibi

5 IMP-2 was shown to be required for efficient proMMP-2 activation both in vivo and in vitro.

6 , which inhibits collagenolytic activity and proMMP-2 activation by MT1-MMP on the cell surface.

7 First, we determined that Con A induces proMMP-2 activation in HT1080 cells by shifting endogeno

8 Despite their difference in mediating proMMP-2 activation in transfected cells, the CAT of MT6

9 ProMMP-2 activation requires multimolecular complex asse

10 A significant up-regulation of MMP-dependent proMMP-2 activation was observed in LPA-treated cells, l

11 Thus, a reciprocal effect on proMMP-2 activation was observed whereupon blocking ERK1

12 hich regulate cell proliferation, apoptosis, proMMP-2 activation, and angiogenesis.

13 ependent invasion proceeded independently of proMMP-2 activation, but required the enzymes to be memb

14 ns of MT1-MMP on the cell surface, including proMMP-2 activation, degradation of gelatin and collagen

15 s only 10% as active as MT5-MMP in mediating proMMP-2 activation, it generates a higher ratio of matu

16 alytic" or enhancing effect on cell-mediated proMMP-2 activation, whereas at higher concentrations, T

17 brane-dependent dimerization is critical for proMMP-2 activation, whereas hemopexin-dependent dimeriz

18 the CAT of prototypical MT1-MMP in mediating proMMP-2 activation.

19 buffer, suggesting that its CAT is a strong proMMP-2 activator.

20 e show that MT6-MMP is capable of activating proMMP-2, an enzyme implicated in tumor invasion and met

21 Bone sialoprotein specifically binds proMMP-2 and active MMP-2, while osteopontin binds proMM

22 or enzyme function in terms of activation of proMMP-2 and binding TIMP-2 to the cell surface (MT1-MMP

23 Activation of proMMP-2 and cell surface collagenolysis are important a

24 Co-transfection of COS-1 cells with both proMMP-2 and furin cDNAs resulted in the cleavage of the

25 red with FLS from DA rats, but the levels of proMMP-2 and MMP-2 messenger RNA in DA.F344(Cia5d) rats

26 1 integrin clustering promotes activation of proMMP-2 and processing of membrane type 1 MMP in ovaria

27 Binding of proMMP-2 and proMMP-2/TIMP-2 to melanoma cells was not i

28 IL-1beta increased the expression of proMMP-2 and proMMP-3 mRNA, suggesting that increased me

29 membrane-type MMP (MT1-MMP), which activates proMMP-2, and to the abundant expression of TIMPs, parti

30 d, indicating that both TIMP-2 and activated proMMP-2 are dispensable for normal development.

31 olecular complex between MMP-14, TIMP-2, and proMMP-2 at the cell surface.

32 ever, dramatically altered the activation of proMMP-2 both in vivo and in vitro.

33 n vitro studies indicated that activation of proMMP-2 can occur through formation of a trimolecular c

34 r resulted in a dose-dependent inhibition of proMMP-2 cleavage; recombinant tissue inhibitor of metal

35 DMEC with anti-MT-MMP-1 antibodies decreased proMMP-2 conversion activity only.

36 sistent with intracellular furin cleavage of proMMP-2 in the trans-Golgi network.

37 P-2 is essential for efficient activation of proMMP-2 in vivo.

38 vasion and metastasis by directly converting proMMP-2 into active form.

39 nsistently, purified CAT of MT6-MMP converts proMMP-2 into mostly the mature form.

40 own that the MT1-MMP-catalyzed activation of proMMP-2 is involved in the outgrowth of cultured lympha

41 s cleaving matrix proteins, MMP-14 activates proMMP-2 leading to an amplification of pericellular pro

42 no acid sequence in the propeptide domain of proMMP-2 leading to inactivation of the enzyme.

43 es multimolecular complex assembly involving proMMP-2, membrane type 1-MMP (MT1-MMP, MMP-14), and tis

44 wed a significant reduction in the levels of proMMP-2, MMP-9, and proMMP-9 in the culture medium.

45 : one with high affinity that is involved in proMMP-2 or hydroxamate-inhibited MMP-2; and the other w

46 ly attenuated maturation of proMMP-9 but not proMMP-2 or proMMP-13.

47 DX-2400 blocked proMMP-2 processing on tumor and endothelial cells, inhi

48 pecific inhibitor NS-398 reduced LPA-induced proMMP-2 protein expression and activation and blocked M

49 in the furin consensus recognition motif of proMMP-2(R69KPR72) prevented propeptide cleavage, thereb

50 partate (NMDA) receptors in RA FLS increased proMMP-2 release, whereas non-NMDA ionotropic glutamate

51 ctivation of pro-matrix metalloproteinase-2 (proMMP-2) required proper glycosylation of MT1-MMP.

52 otype by mediating more robust activation of proMMP-2 than the wild type molecule.

53 ghted different modes of interaction between proMMP-2-TIMP-2 (or TIMP-4) and active MMP-2-TIMP-2 (or

54 Binding of proMMP-2 and proMMP-2/TIMP-2 to melanoma cells was not inhibited by R

55 ssion of MT1-MMP increased the conversion of proMMP-2 to the intermediate and active forms.

56 sly added TIMP-2 could restore activation of proMMP-2 to TIMP-2-deficient cells.

57 in defective formation of the MT1-MMP/TIMP-2/proMMP-2 trimeric activation complex.

58 ity of carbohydrate-free MT1-MMP to activate proMMP-2 was not a result of defective MT1-MMP zymogen a

59 Progelatinase A (ProMMP-2) was present in all the vitreous samples but di

60 inactive mutant MMP-2EA (the E404A mutant of proMMP-2), which cannot autocatalytically mature from th

61 ino acid convertase, furin, directly cleaves proMMP-2 within the trans-Golgi network leading to an in

62 tibody blocks the enzyme ability to activate proMMP-2 without interfering with the collagenolytic fun