ライフサイエンスコーパス: processive

1 ficiently cross linked, and myosin has to be processive.

2 phosphorylation is distributive rather than processive.

3 directing substrates to the distributive or processive 3' to 5' exoribonuclease activities of Rrp6 o

4 Its dominant, processive 5'-3' exonuclease and secondary 5'-flap endon

5 rs based on covalent chemistry and is weakly processive (6 +/- 1 steps per outing).

6 Lin28-stimulated oligo-uridylation, which is processive, a distributive mode is employed by TUT7 for

7 Myosin Va (myoVa) is a processive, actin-based molecular motor essential for in

8 differences between the in vitro apparently processive activity and in vivo distributive monouridyly

9 Pauses during processive activity on both types of DNA exhibited ATP d

10 4 (Dis3) and Rrp6 subunits to modulate their processive and distributive 3'-to-5' exoribonuclease act

11 ntains 10 Sec residues (SEPP1) suggests that processive and efficient Sec incorporation occurs in viv

12 almodulin binding protein)) exhibited highly processive and fast motility (up to 0.6 mum s(-1)).

13 model of DNA replication describes a highly-processive and largely continuous process by which the g

14 ay important regulatory roles in this highly processive and ordered process.

15 tion of ParA DNA binding activity to enforce processive and pole-directed centromere segregation, pre

16 assays, we show that Kif18B is only modestly processive and that the motor switches frequently betwee

17 Compared with kinesin-1, kinesin-2 is less processive, and its processivity is more sensitive to lo

18 NA synthesis during HR is conservative, less processive, and more mutagenic than replicative DNA synt

19 d direct interactions of cargo with a highly processive anterograde motor.

20 Our recent studies showed that KIF3AC is as processive as conventional kinesin-1, suggesting that th

21 hyscomitrella patens and found that none are processive as native dimers.

22 ilaments display alternating phases of rapid processive assembly and arrested growth, driven by a "pi

23 ociation with barbed ends, followed by rapid processive assembly and release of Spire.

24 es Spire from the barbed end to elicit rapid processive assembly from profilin.actin.

25 -tail with DADs from other formins tunes the processive association with the barbed end, indicating t

26 PolD2-PolD3) is much more efficient and more processive at bypassing a 1,2-intrastrand d(GpG)-cisplat

27 acer sequences through a mechanism involving processive, ATP-dependent Cas3 translocation along forei

28 eneral design guidelines to construct highly processive, autonomous DNA walker systems and to regulat

29 The Phi29 DNA polymerase (DNAP) is a processive B-family replicative DNAP.

30 ther drops as rebinding increases and pseudo-processive behavior becomes possible.

31 atalyzed by the glycosyltransferase GlfT2, a processive carbohydrate polymerase, which generates a se

32 in-recruited ZAP-70, which suggests a weakly processive catalysis on membranes.

33 nsideration of DNA looping in the context of processive catalysis provides new mechanistic insights a

34 e enzyme on interfaces and should facilitate processive catalysis.

35 which is of interest for the development of processive catalysts, i.e., catalysts that thread onto p

36 cally and industrially important GH Family 7 processive cellulases with free energy perturbation/repl

37 The molecular mechanism of processive chitin synthesis is not understood, limiting

38 The method was applied to the study of the processive chitinase ChiA from Serratia marcescens.

39 Blunt termini promote processive cleavage, while 3' overhanging termini are cl

40 BLM is significantly less processive compared to the highly efficient GQ destabili

41 anslocates by a disaccharide unit during the processive cycle of a glycoside hydrolase family 7 cello

42 ard directions, and also produce the fastest processive cytoskeletal motor measured so far, reaching

43 approximately 800 nt gRNA precursors, their processive degradation to a mature size of 40-60 nt, and

44 ation solubilizes its outer surface allowing processive degradation.

45 d oligomeric proteins that are essential for processive deoxyribonucleic acid replication.

46 from the substrate interface followed by its processive depolymerization to soluble sugars.

47 s provide new insights into the mechanism of processive digestion that will be discussed.

48 igate the movement of fluorescently labeled, processive, dimeric motor proteins and propose a unified

49 ion between the heads, the model still shows processive directional stepping, but it becomes uncoordi

50 sK/SpoIIIE translocases move DNA in a highly processive, directional manner, where directionality is

51 a-SNAP binding events may occur as part of a processive disassembly mechanism.

52 hich makes the RNA-DNA primers accessible to processive DNA pols.

53 A highly processive DNA polymerase was conjugated to the nanopore

54 ate region accurately and efficiently during processive DNA repeat synthesis has remained elusive.

55 sliding clamp), is rapidly exchanged during processive DNA replication.

56 e DNA and associate with DNA polymerases for processive DNA replication.

57 o be modified as a consequence of disrupting processive DNA replication.

58 A) adopts a ring-shaped structure to promote processive DNA synthesis, acting as a sliding clamp for

59 le recombinant protein that is functional in processive DNA synthesis.

60 DNAP that can couple strand displacement to processive DNA synthesis.

61 w know that PCNA does much more than promote processive DNA synthesis.

62 e and as an essential component required for processive DNA synthesis.

63 Hexameric helicases are processive DNA unwinding machines but how they engage wi

64 t of the conserved domains, YonO is a highly processive DNA-dependent RNA polymerase.

65 We conclude that AdnAB's processive DSB unwinding activity suffices for AdnAB fun

66 These enzyme complexes are highly processive, duplex unwinding and degrading machines that

67 ate, leading to a dramatic increase in fast, processive dynein motility.

68 y and can synergize with dynactin to enhance processive dynein movement.

69 also increases the frequency and velocity of processive dynein movements that are activated by comple

70 offers insight into how cargo adaptors form processive dynein-dynactin motor complexes.

71 py to study the interaction of LIS1 with the processive dynein-dynactin-BicD2N (DDB) complex.

72 in the catalytic cycle based on RNA binding, processive elongation activity, and single nucleotide in

73 al polymerases, was found to be critical for processive elongation and virus growth.

74 ly65Val allele in Li1 plants likely disrupts processive elongation of F-actin, resulting in a disorga

75 eins act as actin polymerases that drive the processive elongation of filament barbed ends in membran

76 Processive elongation of RNA Polymerase II from a proxim

77 d subsequent intermediates leading to highly processive elongation, and we examined the effects of fo

78 om preferential substrate binding but during processive elongation.

79 e transition from distributive initiation to processive elongation.

80 Loop formation followed by processive enlargement provides a mechanism by which con

81 the discovery that the HECT E3 Nedd4-1 is a processive enzyme and that disruption of its processivit

82 ces cerevisiae Pol delta-PCNA is a rapid and processive enzyme, suggesting that CMG may bind and alte

83 from Escherichia coli is a uniquely fast and processive enzyme.

84 terified zones, indicating that it acts as a processive enzyme.

85 CsaB is a distributive enzyme, and CsxA is a processive enzyme.

86 the alginate polymer suggest that AlgG is a processive enzyme.

87 Processive enzymes are major components of the efficient

88 Whereas processive enzymes bind only once to a substrate before

89 ave indicated that kinesin-14 motors are non-processive enzymes, working in the context of multi-moto

90 leotide precision using nanopores coupled to processive enzymes.

91 dicate that this organization helps to drive processive exo-ribonucleolytic cleavage.

92 le by sequentially measuring the products of processive exopeptidase digestion, or by using a molecul

93 RNase II, a 3' to 5' processive exoribonuclease, is the major hydrolytic enzy

94 llulose is produced by cellulose synthase, a processive family-2 glycosyltransferase.

95 Dia is a more processive, faster elongator, paralleling the long, stab

96 This is the direct demonstration of a processive formin from plants.

97 ng cell nuclear antigen and participation in processive genome replication are common features of FAM

98 uggest that B35DNAP may perform faithful and processive genome replication in vivo and, when required

99 heterodimeric co-factor A20.D4 required for processive genome synthesis.

100 obscured by a complex multistep mechanism of processive GHs.

101 Processive glycoside hydrolases (GHs), like cellobiohydr

102 Processive glycoside hydrolases are the key components o

103 ylgalactosyl diacylglycerol synthesized by a processive glycosyltransferase.

104 se is synthesized by membrane-integrated and processive glycosyltransferases that polymerize UDP-acti

105 y electrophoresis shows that this PME is non-processive, halophilic, and acidophilic.

106 Kinesin-1, -2, -5, and -7 generate processive hand-over-hand 8-nm steps to transport intrac

107 The structure suggests a processive hand-over-hand unfolding mechanism, where eac

108 molecule approaches, we show that hDNA2 is a processive helicase capable of unwinding kilobases of ds

109 stead as an adenosine triphosphate-dependent processive helicase when complexed with two accessory pr

110 itial binding, assembly, and function of the processive helicase-primase (primosome) component of the

111 PolC is a processive high-fidelity replicative polymerase, while t

112 osed of Cyclin T1 and CDK9, is essential for processive HIV-1 transcription.

113 We reveal that hKif15 is a plus-end-directed processive homotetramer that can step against loads of u

114 ays revealed that Pol beta scans DNA using a processive hopping mechanism and has a mean search footp

115 is involved in base excision repair, uses a processive hopping search mechanism to locate substrates

116 description of how this channel facilitates processive hydrolysis of unbound cAMP.

117 llably bidirectional myosin that is robustly processive in both forward and backward directions, and

118 core particles revealed that Pol beta is not processive in the context of a nucleosome, and its singl

119 the lagging-strand polymerase is simply less processive in the context of a replisome.

120 s suggest that phosphorylation can be pseudo-processive in the crowded cellular environment, since re

121 PC-mediated ubiquitylation involves a highly processive initial reaction on the substrate, followed b

122 n of (5m)C by CD of mouse TET2 is chemically processive (iterative).

123 KIF3AB is an N-terminal processive kinesin-2 family member best known for its ro

124 Mammalian KIF3AB is an N-terminal processive kinesin-2 that is best known for its roles in

125 KIF16B is a highly processive kinesin-3 family member that participates in

126 e affinity results in a high landing rate of processive kinesin-3 motors on the microtubule surface.

127 odimeric KIF17 and heterotrimeric KIF3AB are processive, kinesin-2 family motors that act jointly to

128 ficant reduction in the distances that these processive kinesins can travel.

129 tuned somewhat differently from homodimeric processive kinesins.

130 ity of KIF3AB in comparison with homodimeric processive kinesins.

131 ize, and a unified expression for predicting processive lifetime is revealed.

132 rocessive myosin ensembles and produce novel processive lifetime measurements.

133 s of prior experimental evidence that longer processive lifetimes are enabled by larger myosin ensemb

134 propose that secondary transport can adopt a processive-like mode of action, thus expanding the subst

135 ion by Mre11-Rad50-Xrs2 and Sae2, as well as processive long-range resection by Sgs1-Dna2 or Exo1 pat

136 plate and synthesize RNA product in a highly processive manner in vitro.

137 has also been reported to methylate DNA in a processive manner, a property that is incompatible with

138 d full deacetylation of (GlcNAc)3-6 in a non-processive manner.

139 quet, thus facilitating homolog pairing in a processive manner.

140 thesize that cleavage proceeds by means of a processive mechanism of substrate insertion, translocati

141 ed by RT0101 or RP027-028, consistent with a processive mechanism of trimethylation.

142 two CpG sites did not provide evidence for a processive mechanism over a wide range of enzyme concent

143 This so-called processive mechanism relies on a complex pattern of enzy

144 first evidence that DHX36 uses a local, non-processive mechanism to unwind G4 substrates, reminiscen

145 dy state kinetic model that accounts for the processive mechanism.

146 the C-terminal domain that would allow for a processive mechanism.

147 Intracellular transport is largely driven by processive microtubule- and actin-based molecular motors

148 pulation undergo either diffusive, or highly processive, minus end-directed movements along microtubu

149 tor that can work alone, to Eg5, a minimally processive mitotic motor that works in large ensembles.

150 maining mutations selectively compromise the processive mode of dynein movement that is activated by

151 hanges the reaction from a distributive to a processive mode.

152 Teams of processive molecular motors are critical for intracellul

153 that unlike conventional myosin-II or other processive molecular motors, Ncd requires two ATP turnov

154 ects motor-microtubule complex stability and processive motility (the ability of an individual motor

155 Their slower chemical kinetics allows processive motility and a high stall force without the n

156 that a variety of adapter proteins activate processive motility by linking dynactin to dynein only w

157 ctional output of the K-loop with respect to processive motility of dimeric kinesin-3 motors is unkno

158 Plus end-directed processive motility of KlpA on single microtubules depen

159 Here, we examined the processive motility of mammalian dynein complexed with d

160 ppears to be well matched to the duration of processive motility of the KIF16B motor, indicating the

161 KlpA exhibits plus end-directed processive motility on single microtubules, but reverts

162 This motor exhibited fast processive motility on single MTs interrupted by pauses.

163 This processive motility requires gating mechanisms to coordi

164 ing of LIS1 to DDB does not strongly disrupt processive motility.

165 lly required for an allosteric activation of processive motility.

166 Central to understanding the processive motion of myosin on actin is the characteriza

167 Paradoxically, this resulting processive motion relies on quenching diffusive plasmid

168 ates that the origin of replication exhibits processive motion with a mean displacement that scales a

169 l of Cin8 is not necessary for bidirectional processive motion, but is controlling the switch between

170 the catalytic domain alone is sufficient for processive motion, providing insight into TrCel7A's mole

171 catastrophe inhibitor in vitro that uses its processive motor activity as part of a feedback loop to

172 tors, as well as with the requirement that a processive motor driving a large load via an elastic lin

173 the collective transport characteristics of processive motor enzymes in crowded conditions.

174 s in the natural intracellular medium, where processive motor enzymes might operate in crowded condit

175 shaped by evolution to cope with the lack of processive motor proteins in bacteria.

176 ess to explore the mechanochemical cycles of processive motor proteins such as kinesin-1, but it has

177 how that the mammalian kinesin-4 KIF21B is a processive motor that can accumulate at microtubule plus

178 Myosin 5c (Myo5c) is a low duty ratio, non-processive motor unable to move continuously along actin

179 he motor domain, KlpA is a plus end-directed processive motor; in contrast, when the tail detaches fr

180 The collective behaviors of certain processive motors, like myosinVa, may therefore be more

181 lity using assembling F-actin and two highly processive motors, myosin-5 and myosin-6.

182 In contrast to other processive motors, stepping of the dynein motor domains

183 Thus, processive motors, which commonly follow individual prot

184 -type Myo52p with small cargoes, can undergo processive movement along actin-Cdc8p cables in vivo.

185 a transport machine capable of long-distance processive movement along microtubules.

186 how that the yeast kinesin-14 Kar3 generates processive movement as a heterodimer with the non-motor

187 We observed that processive movement is rescued if ATP is added to the mu

188 The treadmilling dynamics direct the processive movement of the septal cell wall synthesis ma

189 The data suggest that, during processive movement, tethered-head binding occurs subseq

190 DNA scaffold is an important factor for the processive movement.

191 the coupling of two Myo5c molecules enables processive movement.

192 to a ternary complex (termed DDB) capable of processive movement.

193 tical trap loads, we observed staircase-like processive movements of myosin-10 interacting with the a

194 RNA localisation signals stimulate the processive movements, with regulation of dynein-dynactin

195 The H3K4me3 mark promotes processive, multisite acetylation of histone H3 by Gcn5

196 neously binds a substrate-linked UB to drive processive multiubiquitination.

197 ational techniques are developed to simulate processive myosin ensembles and produce novel processive

198 trongly actin-bound states, similar to other processive myosin motors.

199 sin motor head promotes highly selective and processive myosin V.

200 mechanochemical systems are investigated for processive myosin-actin motility systems.

201 Natural processive myosins are dimeric and use internal tension

202 er, much of our mechanistic understanding of processive myosins comes from in vitro studies in which

203 -dependent ADP release that is essential for processive myosins, such as myosin V.

204 Importantly, our study shows that processive Nedd4-1, but not the distributive Nedd4-1:inh

205 Both human and yeast Exo1 are processive nucleases on their own.

206 corroborated by our observations that during processive nucleotide incorporation, sequential rounds o

207 During processive nucleotide incorporation, the first nucleotid

208 showed that human myosin Vc (MyoVc) was not processive on single-actin filaments [1-3].

209 ecords of motion reveal RNase R to be highly processive: one molecule can unwind over 500 bp of a str

210 We explored the possibility that MyoVc is processive only under conditions that resemble its biolo

211 to determine whether these knots arise by a processive or a dissociative mechanism.

212 val of phosphates can occur in a sequential (processive) or a stepwise (distributive) manner.

213 the initial step and also stimulates the two processive pathways, Sgs1-Dna2 and Exonuclease 1 (Exo1).

214 In contrast to Eg5, diffusive and processive periods were clearly distinguishable.

215 Because the latter monobody inhibits processive phosphorylation by Bcr-Abl and the SH2-kinase

216 harp contrast to the correlated rotations of processive PMEs, and the substrate-binding groove is neg

217 ferent behavior and properties compared with processive PMEs.

218 ication by varphi29 DNA polymerase, a highly processive polymerase enzyme that couples synthesis with

219 ding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains e

220 More importantly, during processive primer extension, pyrophosphate (PPi) release

221 hypothesized that actin cables regulate the processive properties of MyoVc.

222 Active NicD contributes to the non-processive proteolysis and activation of BdlA via phosph

223 sted that individual motors are not robustly processive, raising questions about how dynein-associate

224 This data supports for a processive, rather than distributed model of ERK phospho

225 otein splicing mediated by inteins is a self-processive reaction leading to the excision of the inter

226 Purified human telomerase catalyzes processive repeat synthesis, which could restore the ful

227 w model for DNA and RNA handling dynamics in processive repeat synthesis.

228 This supports the idea that processive replication is required to maintain the histo

229 The chimeric polymerase carries out processive replication with PCNA in vitro; however, in y

230 , which mediate interactions with Pol31, and processive replication with the replication clamp prolif

231 erases depend on circular sliding clamps for processive replication.

232 ation of multiple Sec residues is inherently processive requiring only the SECIS elements but surpris

233 ion during homologous recombination-supports processive resection by Exo1.

234 Although this process is normally processive, resulting in the complete degradation of sub

235 ssociates into smaller particles, and begins processive retrograde IFT in <2 s.

236 ns but lack cytoplasmic dynein, the foremost processive retrograde transporter.

237 PcalRG is the most robust and processive reverse gyrase known to date; it is active ov

238 Processive, ring-shaped protein and nucleic acid protein

239 ndent RNA polymerase (RdRp), exhibits poorly processive RNA synthesis in vitro, at odds with the effi

240 Unlike their QDE-1(Ncr) counterpart favoring processive RNA synthesis, QDE-1(Tte) and QDE-1(Mth) prod

241 To ensure processive RNA synthesis, the viral polymerase L in comp

242 ter to transit into the elongation phase for processive RNA synthesis.

243 ear export dimerizes the motor and activates processive RNA transport.

244 Although polymerization is efficient and processive, RNase H is inefficient and periodic.

245 hen transmitted around the hexamer, reveal a processive rotary translocation mechanism and substrate-

246 ility datasets as a function of sample size, processive run length of the motor, and distribution of

247 KIF3AC heterodimer modulates entry into the processive run regardless of whether the run is initiate

248 this study, the kinetics for entry into the processive run were quantified.

249 KIF3AB heterodimer modulates entry into the processive run, and the results suggest that it is the h

250 uch that either KIF3A or KIF3C initiates the processive run.

251 that either KIF3A or KIF3C can initiate the processive run.

252 cycles of the two motor heads to sustain the processive run.

253 ation of motility during degradation reveals processive runs and distinct steps on the scale of 1 nm.

254 ation selectively destroys the initiation of processive runs at physiological ATP levels.

255 a series of gating mechanisms, which lead to processive runs averaging ~1 mum.

256 -1 function together to support fast, highly processive runs toward axon terminals.

257 gesting that the enzyme can initiate several processive runs without leaving the substrate.

258 e frequent and approximately fivefold longer processive runs, allowing them to catch up with leading

259 Myo1c also regulates the termination of processive runs.

260 Application of our processive search assay into nucleosome core particles r

261 We developed a processive search assay to determine if Pol beta has evo

262 logous DNA Pol X family members to perform a processive search for 1-nucleotide gaps in DNA using a p

263 Of note, the differences in processive searching ability observed among the DNA Pol

264 Processive searching has been reported or proposed for m

265 nes within the lyase domain are required for processive searching, revealing a novel function for the

266 We provide evidence that processive Sec incorporation is linked to selenium utili

267 exact order of these oxidation steps in the processive six-electron biosynthetic reaction is determi

268 nature of conformational changes leading to processive ssDNA translocation and multistranded DNA pro

269 NusG-like proteins to lock RNAP in a closed, processive state during elongation.

270 No signs of processive stepping at the single molecule level were ob

271 cule results presented show that the kinesin processive stepping distance decreases 40-60% with EC50

272 bability of rebinding to F-actin and enabled processive steps along actin filaments, which could be u

273 finity RING-E2 interactions result in highly processive substrate ubiquitination is largely unknown.

274 Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-

275 ep, which appears to be rate-limiting during processive synthesis.

276 nesin-1, with a shorter neck-linker, is more processive than kinesin-2.

277 Thus, although kinesin-1 may be more processive, the longer neck-linker length of kinesin-2 a

278 ses to remove base methylations and a highly processive thermostable group II intron reverse transcri

279 s or small molecules switches Nedd4-1 from a processive to a distributive mechanism of polyubiquitin

280 or of Nedd4-1, which switches Nedd4-1 from a processive to a distributive mechanism.

281 n human ribosomes during single-turnover and processive translation reactions.

282 hree tRNAs, which contributed to the rate of processive translation.

283 ngle molecule manifests during an individual processive translocation event.

284 anopores, a technique to unfold proteins for processive translocation has yet to be demonstrated.

285 is sufficient for gate activation, allowing processive translocation of export substrates for effici

286 illustrated by comparing kinesin-1, a highly processive transport motor that can work alone, to Eg5,

287 otor proteins that is critical for kinesin's processive transport of intracellular cargo along the mi

288 ulating dynein and kinesin motors to promote processive transport.

289 nonmuscle myosin IIB molecules form a highly processive unit when polymerized into filaments.

290 By generalizing a previous model of processive unwinding by helicases, we provide a unified

291 ed double-length SNARE complex, suggesting a processive unwinding mechanism.

292 on requires a RecQ dimer, and that continued processive unwinding of several kilobases involves multi

293 for RNA binding, subunit oligomerization and processive UTP incorporation, and predict druggable pock

294 rm tetramers and provide an amended model of processive VASP-mediated actin assembly in clustered arr

295 ubes and use that energy to fuel autonomous, processive walking through a series of conformational ch

296 bosomes glide forward on the mRNA track in a processive way.

297 limiting reaction regimes, distributive and processive, which differ in the number of enzyme-substra

298 sequence for coiled-coil formation is highly processive with run lengths of approximately 1.23 mum an

299 neck linker and helix alpha7 is also highly processive with run lengths of approximately 1.62 mum an

300 s translocation activity is rapid and highly processive, with individual complexes traveling an avera