ライフサイエンスコーパス: processivity

1 ely due to rapid rebindings (so-called quasi-processivity).

2 and for 17alpha-hydroxylation, suggestive of processivity.

3 of TOP1 above its basal rate, enhancing its processivity.

4 ation forms and arbitrary levels of reaction processivity.

5 -Abetan stabilizers increase gamma-secretase processivity.

6 ing activity or changes in RNA polymerase II processivity.

7 ng that L11 also contributes to tuning motor processivity.

8 oting the docked state to augment telomerase processivity.

9 l and mechanistic property that can modulate processivity.

10 alytic activity, but drastically reduced its processivity.

11 ubule plus ends in a manner dependent on its processivity.

12 at this amino acid on helix 6 contributes to processivity.

13 y include an increase in its repeat addition processivity.

14 e enzymes and have long been associated with processivity.

15 ly affects transcriptional and translational processivity.

16 rNTPs also lower replisome processivity.

17 s on the Escherichia coli replisome rate and processivity.

18 ion is the glycosylation reaction, not chain processivity.

19 which has been implicated in changing Dnmt3a processivity.

20 up studies on the mechanistic basis of motor processivity.

21 ssociation upon nascent chain elongation and processivity.

22 dynein and cargoes and enhances single-motor processivity.

23 o DNA for use by DNA polymerases to increase processivity.

24 role played by the ACP within PKS timing and processivity.

25 ng channel, thereby increasing transcription processivity.

26 racks within the cell by switching on myosin processivity.

27 ne topoisomerase IIalpha relaxation rate and processivity.

28 rate constant but at the expense of reduced processivity.

29 guing that APOBEC3 proteins impede enzymatic processivity.

30 with varied specificity, cooperativity, and processivity.

31 omotes Dyn1 homodimerization and potentiates processivity.

32 l differences in relaxation rate rather than processivity.

33 polarity by promoting anterograde transport processivity.

34 rgy profiles exhibit no barriers for initial processivity.

35 , which may aid in substrate acquisition and processivity.

36 safety leash, may be an adaptation for high processivity.

37 nding, suggesting a structural basis for its processivity.

38 ned by telomerases with lower than wild-type processivity.

39 1 and TEB, but not RPA, increased telomerase processivity.

40 transcription elongation and RNA polymerase processivity.

41 1 gives equivalent enhancement of telomerase processivity.

42 gase, Ube3c/Hul5, which enhances proteasomal processivity.

43 the ssDNA base also influences translocation processivity.

44 tility of kinesin and for the maintenance of processivity.

45 nction, and therefore enhances transcription processivity.

46 le electrostatic interactions also increases processivity.

47 pt4/5, both of which stimulate transcription processivity.

48 ng and diminished telomerase repeat addition processivity.

49 TPase cycle to produce functional changes in processivity.

50 esulted in increased off-rates and decreased processivity.

51 s can be realized at high levels of reaction processivity.

52 and to stimulate telomerase repeat addition processivity.

53 in mediating ubiquitin transfer and reaction processivity.

54 s leading to loss of telomerase activity and processivity.

55 in determining motor velocity, pausing, and processivity.

56 However, all three MCM proteins have similar processivities.

57 and R346M, were found to decrease kinesin-3 processivity 10-fold and thus approach kinesin-1 levels.

58 This modification increased in vitro processivity 4-fold as compared to the wild-type Dbh.

59 n shown to impair telomerase association and processivity [8-10].

60 omerase synthesizes TTAGGG repeats with high processivity, a common attribute of vertebrate telomeras

61 Unexpectedly, differential processivity alone could not explain the differential fa

62 In contrast, the unwinding processivity always increases when the force increases.

63 ls to suggest experiments to 1) detect quasi-processivity and 2) test the theory.

64 d structure-function relationships governing processivity and a better understanding of the potential

65 th respect to telomerase enzymatic activity, processivity and activation by telomere proteins.

66 d loose active site, but are limited by poor processivity and activity.

67 1 also appears to digest chromatin with high processivity and at rates similar to naked DNA in vitro.

68 This interaction increases both the processivity and catalytic efficiency of the error-free

69 bility to rationally design increases in pol processivity and catalytic efficiency through computatio

70 The sliding clamp enhances polymerase processivity and coordinates DNA replication with other

71 s in vitro to identify factors that regulate processivity and efficiency.

72 signals and generally increase transcription processivity and elongation rate, contributing for trans

73 ces of changes in telomerase repeat addition processivity and expression level on telomere elongation

74 like transcript cleavage factors enhance the processivity and fidelity of archaeal and eukaryotic RNA

75 activity, the zinc finger also regulates the processivity and fidelity of PrimPol's extension activit

76 e a kinetic framework for explaining kinesin processivity and for mapping structural differences to f

77 ingle nucleotide at a normal rate, they lack processivity and have reduced repetitive exonuclease rat

78 ever, the underlying mechanism for this high processivity and how stepping is affected by force are u

79 iants with mutations in motifs implicated in processivity and interaction with DNA, namely the insert

80 to be addressed in molecular descriptions of processivity and its driving forces.

81 eta and the exonuclease epsilon that provide processivity and proofreading, respectively.

82 tion using lambda-exonuclease, including its processivity and reaction rate, were higher when the enz

83 ution or when clustered on a surface, albeit processivity and resistance toward capping protein (CP)

84 esigned unique kinetic assays to analyze the processivity and stability of the pol delta holoenzyme.

85 bility of tunnel loops, which likely affects processivity and substrate complexation.

86 ture engineering is required to overcome the processivity and the intrinsic DNA binding affinity of d

87 tion supporting enhanced ATPase activity and processivity and the other supporting nucleotide excisio

88 ination between motor heads is essential for processivity and the variation of peripheral structural

89 lication primers and increases transcription processivity and thereby serves as a molecular switch be

90 nome-wide analyses that show high RNA Pol II processivity and transcription activity at MSL target ge

91 the newly synthesized DNA, that cause lower processivity and transient lagging-strand polymerase dis

92 NL length is tuned to enhance unidirectional processivity and velocity.

93 how the E. coli replisome can maintain high processivity and yet possess the flexibility to bypass o

94 nzymes exhibit low RNA binding activity, low processivity, and alterations in nucleotide recognition,

95 concerted mode to regulate RNA replication, processivity, and fidelity.

96 we characterized the substrate specificity, processivity, and kinetic mechanism of bacterially expre

97 measurements of binding strength, fidelity, processivity, and overall efficiency.

98 pect to binding affinity, on- and off-rates, processivity, and synergism with endoglucanase.

99 protein interactions to guide reactivity and processivity, and their control has become one of the ma

100 Although small differences in processivity are likely obscured in vivo by the coupling

101 the negative effects of primase and rNTPs on processivity are overcome by the extra grip on DNA provi

102 threshold levels of telomerase activity and processivity are required to maintain telomere function

103 he ATPase, microtubule-binding affinity, and processivity are unchanged between a phosphomimetic S175

104 Processivity arises from a high ratio of MT-bound to unb

105 iological analyses implicate repeat addition processivity as essential for telomerase function.

106 is much larger), interhead coordination, and processivity, as reported for mammalian dyneins.

107 -terminal extension in CsxA, which conferred processivity because of the existence of a second produc

108 define the key principles that underlie its processivity but also to define the mechanistic basis of

109 find that primase activity lowers replisome processivity but only when lagging strand extension is i

110 lation mechanism driven by general cellulase processivity, but also excavates extensive cavities into

111 processive enzyme and that disruption of its processivity by biochemical mutations or small molecules

112 Additionally, NAP1L1 increases CSB processivity by decreasing the pausing probability durin

113 nslocation based on the regulation of dynein processivity by discrete cargo-associated features.

114 ccessory subunit binds to p140 and increases processivity by preventing polgamma's dissociation from

115 evidence that BICD2N and dynactin stimulate processivity by regulating individual dynein complexes,

116 scherichia coli NusG increases transcription processivity by suppressing RNAP pausing, whereas Bacill

117 Here, we show that processivity can be enhanced in engineered myosins using

118 vestigated the role of the Bacillus subtilis processivity clamp DnaN, and found that it serves as a p

119 recombination intermediates dependent on the processivity clamp PCNA.

120 Using variants of MutS and the replication processivity clamp to impair mismatch repair, we find th

121 ymerase Pol III core complex and the dimeric processivity clamp, beta.

122 yotes, and both cases are coordinated by the processivity clamp.

123 Processivity clamps are vital components of DNA replicat

124 Ring-shaped processivity clamps play a critical but ill-defined role

125 ther FEN family members can be stimulated by processivity clamps such as proliferating cell nuclear a

126 A:DNA heteroduplexes with moderately greater processivity compared with its duplex DNA:DNA counterpar

127 nstitute reduced levels of DNA synthesis and processivity compared with wild-type telomerase.

128 Loss of processivity could be due to inability to hold on to the

129 ongation, loss of EAF5 or EAF7 resulted in a processivity defect.

130 Although heterozygous processivity defects have been associated with familial,

131 Kinesin processivity, defined as the average number of steps tha

132 ve-site cavity important for repeat addition processivity, determine how they contribute to duplex an

133 e than kinesin-1 to external forces, but its processivity diminishes steeply with load, and the motor

134 Furthermore, telomerases with low processivity dramatically elongate telomeres when overex

135 mporally controls RNA polymerase II (Pol II) processivity during transcription elongation through cyc

136 es, which has implications for the degree of processivity, endo-initiation, and substrate dissociatio

137 ved with helicases to increase the unwinding processivity even if the velocity remains unaffected.

138 tive catalytic activity, and compromised DNA processivity, even in the presence of the p55 accessory

139 NA mismatch, MutS, MutL, and the replication processivity factor (beta-clamp or PCNA) activate the la

140 at interactions with proteins other than the processivity factor beta play a role under certain condi

141 oliferating cell nuclear antigen (PCNA), the processivity factor for DNA replication, plays a central

142 f replication protein A (RPA), MRN acts as a processivity factor for Exo1, retaining the exonuclease

143 by bacteriophage Xp10 acts as an elongation processivity factor of RNAP of host bacterium Xanthomona

144 oduct 45 (gp45, also known as DNA polymerase processivity factor or sliding clamp) obtained during ch

145 s leading-strand synthesis together with its processivity factor PCNA.

146 dulated by the nontemplate strand and by the processivity factor Spt4/5, both of which stimulate tran

147 gesting the 9-1-1 complex acts as a nuclease processivity factor to facilitate extensive resection.

148 protein A (RPA) functionally cooperate as a processivity factor to stimulate HCV replication by HCV

149 e, two DNA polymerases, and two thioredoxin (processivity factor) molecules was determined by single-

150 An in silico model of the vacinnia virus processivity factor, comprised of the A20 and D4 heteroc

151 c functions require the dynein activator and processivity factor, dynactin.

152 pted by BPLF1, which deubiquitinates the DNA processivity factor, PCNA, and inhibits the recruitment

153 omain, decreasing interaction with the beta2 processivity factor.

154 terodimer that functions as the poxvirus DNA processivity factor.

155 Viral processivity factors are attractive drug targets in bein

156 ng clamps onto DNA where the clamps serve as processivity factors for DNA polymerases.

157 roteins, homologs of Clip170 and EB1, act as processivity factors for Kip2, helping it overcome dynei

158 implying that viral selection had optimized processivity failure.

159 partial or complete loss of repeat addition processivity for telomere maintenance.

160 tical for binding of ORF59 to oriLyt and its processivity function.

161 Such high processivity has not been observed for any other motor p

162 he molecular details of polysaccharide chain processivity have not been elucidated.

163 ) memory T cells increases RNA polymerase II processivity, histone acetylation, and baseline HIV-1 tr

164 DNA polymerase (pol) processivity, i.e., the bases a polymerase extends befor

165 deletion of the TPR2 subdomain, required for processivity, impaired primer extension beyond the abasi

166 understand how to rationally design enhanced processivity in a Y-family pol.

167 r length has been shown to directly modulate processivity in different kinesin families; for example,

168 ceeds with an effectively uniform conversion processivity in either direction limited by the bounds o

169 asis toward a general theory of carbohydrate processivity in GHs and other enzymes able to process li

170 proteins for both telomerase recruitment and processivity in mammalian cells [5-7].

171 However, we observed processivity in pregnenolone/DHEA pulse-chase experiment

172 nism by which ATP-dependent proteases attain processivity in protein degradation.

173 odels confirmed the reaction order, revealed processivity in the phosphorylation of MEK6 by ASK1, and

174 port this finding by demonstrating defective processivity in the previously reported p.K570N T-motif

175 re length and telomerase nucleotide addition processivity in vivo.

176 ith decreased velocity, dramatically reduced processivity, increased monomerization, aggregation, and

177 Processivity inhibition is proposed to be a basis of hum

178 Its processivity is >30,000 nt without significant intermedi

179 Therefore, inhibition of E3 ligase processivity is a viable strategy to design E3 inhibitor

180 more, when the tail is removed, we find that processivity is compromised.

181 ts chromatin by active loop extrusion, whose processivity is controlled by Wapl.

182 We find that greater processivity is correlated with faster attachment of the

183 Its remarkable processivity is critical for ferrying cargo within the c

184 leading to a mechanochemical cycle in which processivity is determined by the race between unbinding

185 Because processivity is difficult to predict analytically and me

186 rvations, our results suggest that degree of processivity is directly correlated to the binding free

187 Here, we hypothesize that the degree of processivity is directly linked to the ability of an enz

188 Interestingly, its processivity is mainly required for resistance to inters

189 sin-1, kinesin-2 is less processive, and its processivity is more sensitive to load, suggesting that

190 inherently distributive enzyme but that some processivity is present, i.e. some of the 17alpha-OH pre

191 in trans, uncoupling it from the fork, high processivity is restored.

192 l element believed to regulate the degree of processivity is the neck-linker, a short peptide of 12-1

193 y between DNA break repair and transcription processivity is unclear.

194 ts inefficient reactions, rather than pseudo-processivity itself.

195 y is more sensitive to load, suggesting that processivity may be controlled by different gating mecha

196 on and suggest that similar factor-dependent processivity may be shared by other members of the DEAD-

197 le-stranded DNA, and the increased unwinding processivity may contribute directly to the antimutator

198 properties of the motor, we show that dynein processivity minimally requires a single active head and

199 ersegmental hopping contributes to enzymatic processivity (multiple modifications), which invoke rece

200 est telomeres, sole expression of telomerase processivity mutants can lead to a profound failure of t

201 clinical and functional impact of biallelic processivity mutations.

202 An enzymatic processivity of 42 nucleotides for a template of the sam

203 The diminished processivity of APOBEC3H haplotype V did not result in a

204 e activity of TFIIH suggests that Ssl2 has a processivity of approximately one DNA turn, consistent w

205 tively, our results suggest that compromised processivity of cargo-motor assemblies contributes to hu

206 to act as a sliding platform for increasing processivity of cellular replicases and for coordinating

207 The variant SdbhM76I further improved the processivity of Dbh by 10 fold.

208 The CSN is essential for the processivity of deep end-resection-the initial step in H

209 s to the proteasome gave substantially lower processivity of degradation than ubiquitin-dependent tar

210 sm by which substrate sequences regulate the processivity of degradation.

211 key parameters that govern the kinetics and processivity of DNA enzyme-based walkers.

212 lizes DNA structure and reduces fidelity and processivity of DNA synthesis.

213 (SHE) complex on DNA, effectively increasing processivity of DNA synthesis.

214 estrating this competition as well as in the processivity of Fmn2.

215 We showed that activity and processivity of hTERT-T726M failed to be stimulated by T

216 efining the key principles that underlie the processivity of KIF3AB in comparison with homodimeric pr

217 Full processivity of L requires its phosphoprotein cofactor a

218 an genomes can be co-methylated owing to the processivity of methyltransferases or demethylases, yet

219 The speed and processivity of Mfd dictate a "release and catch-up" mec

220 or neurons expressing FUS, both motility and processivity of mitochondrial axonal transport were redu

221 that of elongation factors that enhance the processivity of multisubunit RNA polymerases.

222 cuss the implications of this load-dependent processivity of myosin-10 as a filopodial transport moto

223 This higher processivity of Pif1 is attributed to slower dissociatio

224 ragment synthesis in humans as it limits the processivity of pol delta on undamaged DNA and promotes

225 emplate is a key determinant of the rate and processivity of protein synthesis.

226 low rate of tandem repeat synthesis but high processivity of repeat addition, indicative of high stab

227 at is potentiated by RMI1-RMI2, and that the processivity of resection is reliant on the Topo IIIalph

228 it positive transcription factor b, and poor processivity of RNA polymerase II (RNAP II).

229 re, we observe that both factors enhance the processivity of RNA polymerase II through the nucleosoma

230 t in SNM1B and explains the greater apparent processivity of SNM1A.

231 l concentration of E2 enzymes and permit the processivity of substrate ubiquitination.

232 relation between chromatin accessibility and processivity of ten-eleven translocation (TET) enzymes.

233 C distribution maps indicates that catalytic processivity of TET enzymes correlates with local chroma

234 Here, we examined in detail the processivity of the 17alpha-hydroxylation and lyase step

235 AtPOT1a stimulates repeat addition processivity of the canonical telomerase enzyme, while A

236 ancing both its capacity to bind DNA and the processivity of the catalytic domain.

237 between the two heads is the essence of the processivity of the cycle.

238 ctivity of cardiac myosin was able to rescue processivity of the D179Y mutant dimers at physiological

239 However, the processivity of the DNA helicase might overcome DNA gyra

240 of the HG substrate but does not inhibit the processivity of the enzyme.

241 tures presented here suggest a mechanism for processivity of the holoenzyme and provide a model for u

242 ces binding of Pol gamma to DNA and promotes processivity of the holoenzyme.

243 rationale for an optimal NL length to ensure processivity of the motor.

244 ta suggest that in addition to enhancing the processivity of the polymerase PCNA is an allosteric mod

245 ible with weak interactions required for the processivity of the polymerase.

246 that P serves to enhance the initiation and processivity of the RdRP.

247 The ubiquitous sliding clamp facilitates processivity of the replicative polymerase and acts as a

248 The processivity of the ubiquitylation reaction necessitates

249 vity of UvrD303 is due to an increase in the processivity of the unwinding reaction.

250 s with DI-RNA generation and may enhance the processivity of the viral polymerase.

251 rental virus, suggesting that C enhances the processivity of the viral polymerase.

252 ly hydrolyzed by the enzyme, leading to high processivity of the walker movement along the track.

253 Processivity of their polymerase is only achieved when t

254 eractions in the membrane directly influence processivity of ubiquitin attachment to modulate polyubi

255 howed increased off-rates and lower apparent processivity on alpha-chitin.

256 understanding the dependence of velocity and processivity on force and the nucleic acid sequence.

257 se hominid A3C enzymes, dimerization enables processivity on single-stranded DNA and results in highe

258 Optimal processivity on ssDNA also required dimerization of APOB

259 ease, but the impacts of different levels of processivity on telomere maintenance have not been exami

260 In addition, altering the processivity or proofreading activity of DNA polymerase

261 of SnAC does not adversely affect the rate, processivity, or pulling force of SWI/SNF to translocate

262 Despite the variations in processivity, our studies point to possible common patte

263 The intrinsic processivity (P(Intr)) of cellulases has been shown to b

264 llin neddylation, or conform to the reaction processivity paradigms, suggesting that SCCRO3 does not

265 n complex stimulates telomerase activity and processivity provide incentive for testing variant telom

266 Telomerase exhibits repeat addition processivity (RAP) and must therefore translocate the na

267 Therefore, the mechanism of dynein processivity remains unclear.

268 munication is tuned to achieve the degree of processivity required for cellular function.

269 simulations suggest that, rather than KIF3A processivity resulting from strain-dependent regulation

270 e causes specific defects in repeat-addition processivity, revealing that the template recognition el

271 In addition, our processivity studies demonstrate that NTMT1 proceeds via

272 - or mixed-linkage chains leading to greater processivity than K63-linked chains.

273 k, and consequently have a maximum speed and processivity that is three orders of magnitude greater t

274 ea and has revealed a novel mode for dynamic processivity that occurs by a polymerase exchange mechan

275 hemical studies, a molecular-level theory of processivity that relates directly to structural feature

276 High processivity, the ability to take many steps along a tra

277 Despite decreased processivity, the elongation rate of filaments is unchan

278 Without a loss of processivity, the nsp7/nsp8/nsp12 complex can associate

279 A hallmark of class-V myosins is their processivity--the ability to take multiple steps along a

280 cule without dissociating, a feature called "processivity." Therefore, it was surprising when kinetic

281 We speculate that the weak motifs enable processivity through avidity effects and by providing st

282 ring Exo1 to the DNA substrate, PCNA confers processivity to Exo1 in resection.

283 We predict that the sensitivity of unwinding processivity to external force is a universal feature th

284 ring responses of the unwinding velocity and processivity to force have lacked explanation.

285 a downstream "sliding clamp," providing high processivity to the EC.

286 i replisomes have attributed this remarkable processivity to the high stability of the replisome once

287 t SARS-CoV nsp7 and nsp8 activate and confer processivity to the RNA-synthesizing activity of nsp12.

288 synthesizes DNA distributively and with low processivity, unlike most other well-characterized DNA p

289 bits high levels of rebinding-induced pseudo-processivity, unlike other proposed advantages of the du

290 We further investigated processivity using single-turnover experiments, which sh

291 he unwinding process, and helicase rates and processivities were measured as a function of GTP concen

292 led that the overall polymerization rate and processivity were higher for Pol than for Pol2core.

293 Dda helicase exhibited greater processivity when unwinding a DNA fork compared to a ss/

294 oints using the same value of the conversion processivity, which is the only kinetic parameter of the

295 The L-P complex lacks full processivity, which we interpret to reflect the lack of

296 compromises both telomerase recruitment and processivity, while the missense mutation in the TIN2-bi

297 Interestingly, the increase in unwinding processivity with force depends on whether the helicase

298 e, which explains why A3G displays almost no processivity within a 5'-CCC motif.

299 and S1 RNA-binding domains can reduce enzyme processivity without affecting the velocity.

300 single-stranded DNA (ssDNA), it has kilobase processivity; yet, it is unable to processively unwind l