ライフサイエンスコーパス: product inhibition

1 f Gbetagamma is that it relieves competitive product inhibition.

2 the destruction of AdoHcy, thus alleviating product inhibition.

3 tion slows down at higher conversions due to product inhibition.

4 uding "approach to equilibrium" kinetics and product inhibition.

5 ltransferases undergo marked AdoHcy-mediated product inhibition.

6 His-30 and the evolutionary conservation of product inhibition.

7 small molecule production, and resistance to product inhibition.

8 active-site titration, product analysis, and product inhibition.

9 l phosphates that avoids the complication of product inhibition.

10 then promotes a competing pathway leading to product inhibition.

11 differential substrate turnover rather than product inhibition.

12 the biphasic kinetics are not the result of product inhibition.

13 luding unfavorable equilibrium positions and product inhibition.

14 ation of nicotinate mononucleotide and shows product inhibition.

15 ucose 6-phosphate, and phosphate reversal of product inhibition.

16 sms of IKKalpha and IKKbeta by substrate and product inhibition.

17 or Va and factor VaLEIDEN, and the effect of product inhibition.

18 m in which the zero-order phase results from product inhibition.

19 ype MnSOD, which can account for its lack of product inhibition.

20 ed in the regulation of these enzymes by CTP product inhibition.

21 he high affinity site associated with potent product inhibition.

22 Michaelis-Menten kinetics, with competitive product inhibition.

23 ite, which suggested that Ape1 might exhibit product inhibition.

24 erevisiae is allosterically regulated by CTP product inhibition.

25 under physiological conditions and minimizes product inhibition.

26 anes enriched in PI(4,5)P2 and is subject to product inhibition.

27 s endpoint was shown to be the result of end product inhibition.

28 icating that the "burst" phase is not due to product inhibition.

29 tivities of these subunits are regulated via product inhibition.

30 d bacterial MnSODs, due to very low level of product inhibition.

31 roxide (O(2)(-)), due to different levels of product inhibition.

32 sis and has a significant role in regulating product inhibition.

33 chain conformations may affect the extent of product inhibition.

34 nalis PNP-catalyzed reactions, determined by product inhibition and equilibrium isotope exchange, was

35 ts at position 143 showed very low levels of product inhibition and favored Mn(II)SOD in the resting

36 g state, whereas the wild type showed strong product inhibition and favored Mn(III)SOD.

37 he kinetic mechanism of GFS as determined by product inhibition and fluorescence binding studies is c

38 Mn-SOD, which exhibits significantly reduced product inhibition and increased enzymatic efficiency.

39 Product inhibition and isotope partitioning studies supp

40 ls may use different mechanisms to alleviate product inhibition and modulate cholesteryl ester biosyn

41 nism under initial rate conditions; however, product inhibition and product accumulation led to PglH

42 other mechanisms sufficiently alleviate TDG product inhibition and promote its enzymatic turnover in

43 PafA has a much weaker product inhibition and slightly higher activity relative

44 Product inhibition and solvent isotope effects were also

45 The use of dead-end and product inhibition and solvent-isotope effect reveals th

46 e data suggest that GOAT is subjected to end-product inhibition and this inhibition is better achieve

47 required to curtail substrate saturation and product inhibition and to allow efficient catalysis.

48 In order to investigate the mechanism of product inhibition and whether it is a feature common to

49 ts from non-linear kinetic time courses with product inhibition and/or substrate depletion.

50 gh catalytic proficiency, lack of observable product inhibition, and ability to hydrolyze both cocain

51 These include allosteric regulation, product inhibition, and covalent modification as well as

52 ed to perform steady-state initial velocity, product inhibition, and dead end inhibition experiments,

53 Initial velocity, product inhibition, and dead-end analog inhibition studi

54 ns were investigated using initial velocity, product inhibition, and dead-end inhibition analyses.

55 Initial velocity, product inhibition, and dead-end inhibition studies indi

56 Initial velocity studies, product inhibition, and dead-end inhibition studies indi

57 Initial velocity, product inhibition, and dead-end inhibition studies prov

58 Using a combination of initial velocity, product inhibition, and dead-end inhibition studies, the

59 Initial velocity, product inhibition, and deuterium kinetic isotope effect

60 A series of steady-state kinetic, product inhibition, and direct binding studies with Myco

61 Steady-state kinetics, product inhibition, and isotope exchange studies are con

62 This lowers product inhibition, and the target DNA or RNA thus becom

63 nd alcohol substrates, the aldehydes exhibit product inhibition, and we propose that this is due to n

64 ly, both the mt- and plPDCs are sensitive to product inhibition, and, potentially, to metabolite effe

65 udies, including kinetic isotope effects and product inhibition, are discussed in light of this kinet

66 The inefficient removal of product inhibition associated with ADP accounts for the

67 bstrate concentration because of the acetate product inhibition at each temperature.

68 e at the N-terminal half, the site of potent product inhibition at the C-terminal half, and a seconda

69 We describe an approach to study product inhibition at the single-molecule level.

70 catalytic dwell consistent with competitive product inhibition but also decreased the angular veloci

71 tetrahydrofolate synthase not only abolishes product inhibition but also increases the initial rate o

72 leotide and to elucidate the kinetic scheme, product inhibition by 8-oxo-dGMP and dGMP and direct bin

73 Product inhibition by adenine was noncompetitive against

74 tivity at pH 6.5 by preventing substrate and product inhibition by ATP and ADP, respectively.

75 aspects: (1) p50RhoGAP displays an effect of product inhibition by binding to the GDP-bound form of R

76 A simple kinetic model explains how product inhibition by CO(2) leads to overall first-order

77 ts, Bi-substrate kinetic analysis, authentic product inhibition by coenzyme A (CoA) and acetylated H3

78 the possibility of elucidating the origin of product inhibition by comparing human MnSOD with ScMnSOD

79 rylated CTP synthetase was less sensitive to product inhibition by CTP.

80 in a decrease in the enzyme's sensitivity to product inhibition by CTP.

81 uanosine at much higher K(m) values, and end-product inhibition by dCTP.

82 ctedly, our results also uncover significant product inhibition by deconjugated Cul1, which results f

83 can "activate" IDH phosphatase by reversing product inhibition by dephospho-IDH.

84 the metal stoichiometry at the active site, product inhibition by GDP, a potent competitive inhibito

85 6-P of 7 microm, and was highly sensitive to product inhibition by GlcN-6-P.

86 yldolichol (GlcNAc-GlcNAc-P-P-dolichol), and product inhibition by GlcNAc-P-P-dolichol itself.

87 ehydrogenase is also known to exhibit strong product inhibition by H4PteGlu5.

88 It alleviates product inhibition by L-Cth and revealed that the values

89 noyl-CoA, and dodecenoyl-CoA substrates, and product inhibition by lauroyl-CoA suggest that this regi

90 DHPS in plants is highly sensitive to end-product inhibition by lysine and, therefore, has an impo

91 The Ki values for R111M and R84M product inhibition by mevalonate 5-diphosphate are infla

92 The mechanism and magnitude of product inhibition by monophosphorylated EtsDelta138 is

93 ad-end inhibition by AMP and lumichrome, and product inhibition by NAD(+) indicated an ordered sequen

94 observed in the double-reciprocal plots for product inhibition by NADH and the dead-end inhibition b

95 Product inhibition by NADP is competitive vs NADPH and n

96 hat is zero order in superoxide and due to a product inhibition by peroxide anion.

97 ion, the mutant Y34F was more susceptible to product inhibition by peroxide than the wild-type enzyme

98 6-phosphate, and allosterically relieved of product inhibition by phosphate.

99 terminal half is necessary for the relief of product inhibition by phosphate.

100 Kinetic analysis of product inhibition by recombinant SPN demonstrated an or

101 Product inhibition by S-adenosylhomocysteine was competi

102 Product inhibition by saccharopine is uncompetitive vers

103 relief of the methylation reaction from end product inhibition by SAH and the subsequent increase in

104 oned DNA into neutral genomic sites prevents product inhibition by self-replicating plasmids, and has

105 of polyglutamate derivatives formed, and end-product inhibition by the major reduced folylpentaglutam

106 ein scission, and (ii) to prevent subsequent product inhibition by the NS3 C terminus.

107 r ADP, facilitating the efficient removal of product inhibition by this nucleotide.

108 This phenomenon arises from competitive product inhibition by thrombin, which binds to prothromb

109 ociation of IDE with cellular regulators and product inhibition by Ub1-72 can prevent inadvertent pro

110 In an example of end-product inhibition, cholesterol accelerates the proteaso

111 The rate constant for formation of the product inhibition complex also decreases but to a much

112 ty is high and is held back only by a strong product-inhibition component to the catalytic process.

113 When an acetate product inhibition constant of 600+/-31M(-1), determined

114 nd apparent values for Km that depend on the product inhibition constant.

115 sented showing how the variation in apparent product inhibition constants (Kii) can be used to predic

116 Analysis of the product inhibition curve for 10-formyltetrahydrofolate d

117 nt with a Theorell-Chance BiBi mechanism and product inhibition data supported sequential binding of

118 Two-substrate, dead-end and product inhibition data, using analogues of ATP, are con

119 Initial velocity, product inhibition, dead-end inhibition, and equilibrium

120 proline residues in a manner that diminishes product inhibition during collagen biosynthesis.

121 In addition, a product inhibition effect by ADP-ribose (through the reo

122 hich single mutations had little effect, was product inhibition eliminated in HKI.

123 hibited by peroxide, Q143N MnSOD exhibits no product inhibition even at concentrations of O2.

124 TP lid, which otherwise traps ADP, to remove product inhibition exerted by this nucleotide.

125 pectroscopy, bisubstrate kinetic assays, and product inhibition experiments to demonstrate that hNaa5

126 ta, along with equilibrium sedimentation and product inhibition experiments, suggest that steps invol

127 as investigated in initial rate kinetics and product inhibition experiments.

128 t in a mutant variant of Tp47 that overcomes product inhibition for the beta-lactamase activity, a no

129 how clear evidence for previously unreported product inhibition for the LuxAB reaction.

130 omponent of cellulase action on cellulose is product inhibition from monosaccharide and disaccharides

131 Product inhibition generates non-linearity in steady-sta

132 that of the wild-type enzyme and a level of product inhibition greater by approximately 2-fold.

133 trate (Pro) by only a single oxygen atom, no product inhibition has been observed for P4H.

134 on for analysis of kinetic time courses with product inhibition have been put forth.

135 onserved, active-site residue Tyr34 mediates product inhibition; however, the protein environment of

136 hand, the corresponding mutations eliminate product inhibition in a truncated form of HKI, consistin

137 ed inhibition of the protease, demonstrating product inhibition in addition to and distinct from subs

138 To investigate the mechanism of product inhibition in MnSOD, two yeast MnSODs, one from

139 ing the reaction product ADP, which explains product inhibition in mPKs.

140 perfamily of enzymes, and forms the basis of product inhibition in Mug.

141 ecDHFR, an evolutionary adaptation to reduce product inhibition in the NADP+ rich environment of prok

142 determined that kcat/K(m) was decreased and product inhibition increased for H30V MnSOD, both by 1-2

143 An analysis of the patterns of product inhibition indicated that RhlI catalyzes signal

144 2,6-bisphosphatase activity was sensitive to product inhibition; inhibition by inorganic phosphate wa

145 S3 (Solyc08g014230) encodes a functional end product inhibition-insensitive version of the committing

146 No product inhibition is observed, and competitive binding

147 Initial velocity and product inhibition kinetic data are consistent with an o

148 Steady-state initial velocity and product inhibition kinetic studies indicate an ordered B

149 , but suggest that other mechanisms, such as product inhibition, may also play a role in silencing in

150 Product inhibition negatively impacts cellulase action,

151 rt that the ARE genes are not subject to end product inhibition; neither ARE1 nor ARE2 transcription

152 can be directly applied to the phenomenon of product inhibition observed in natural Diels-Alderase en

153 The patterns of product inhibition of AKT1, AKT2, and AKT3 by ADP were a

154 d to proteins is large enough to account for product inhibition of both pyridoxal kinase and pyridoxi

155 Finally, consistent with known product inhibition of CE-rich HDL by CE, CG simulations

156 consolidating process steps and reducing end-product inhibition of enzymes compared with separate hyd

157 also occurred in these experiments, although product inhibition of GAD(67) by increased GABA could pl

158 hesis is regulated, at least in part, by end-product inhibition of glutamine PRPP amidotransferase.

159 versus CHB-glutathione conjugate: formation, product inhibition of GSTA1-1 catalysis, and transport b

160 For MLP detoxification, where product inhibition of GSTA1-1 is less important, GSTA1-1

161 hypotheses have been proposed to account for product inhibition of HKI.

162 , although larger amounts led to competitive product inhibition of hydrolytic activity.

163 enosylhomocysteine (AdoHcy), which causes by-product inhibition of methyltransferases (MTase's).

164 broblasts from affected individuals, and end-product inhibition of PSS1 by phosphatidylserine was mar

165 When neglecting acetate product inhibition of the acylase, values for k(cat) wer

166 ditions, ADP competes with ATP, resulting in product inhibition of the ATPase rate.

167 resistance to CHB in order to relieve potent product inhibition of the enzyme by intracellular CHB-SG

168 ingle mutants could be due to alleviation of product inhibition of the enzyme.

169 te of cellular methyltransferases results in product inhibition of the enzyme.

170 The high stereoselectivity and minimal product inhibition of the evolved enzyme enabled prepara

171 Patterns of product inhibition of the forward reaction were consiste

172 Evidence was also obtained for product inhibition of the protease by the cleaved C term

173 kinetic mechanisms in terms of substrate and product inhibition of the recombinant human (rh) protein

174 or dCTP (not deoxyguanosine or dGTP) and end-product inhibition of the respective activities by dATP

175 isoforms in a 5-fold excess of 18:1-ACP show product inhibition of up to 73%.

176 This correlates with the enhanced product inhibition of Y34F during the catalysis of O-(2)

177 a suggest that elevated levels of pGpG exert product inhibition on EAL-dependent PDEs, thereby increa

178 In vitro analysis showed strong product inhibition on the T507A mutant with particular s

179 tro tests of possible activity regulation by product inhibition or by Akt1 binding gave negative resu

180 o-order kinetic behavior reflect competitive product inhibition or self-inhibition by substrate.

181 Kinetic and product inhibition parameters are derived by converting

182 We recently determined a product-inhibition pathway for the diguanylate cyclase r

183 re qualitatively similar with respect to the product inhibition patterns and the pH dependence of kin

184 The steady-state initial velocity and product inhibition patterns are consistent with an order

185 was also followed when 3-PGA was absent, but product inhibition patterns changed dramatically.

186 In this work, product inhibition patterns for the synthase activity of

187 rst, two-substrate steady-state kinetics and product inhibition patterns indicated a Steady-State Ord

188 The substrate specificities and product inhibition patterns of haloalkane dehalogenases

189 onsideration of the expanded set of reaction products, inhibition rate constants were measured for a

190 Of note, we also found that glucose product inhibition regulates the activities of both SI s

191 ted in a mutant of human MnSOD with weakened product inhibition resembling that of E. coli MnSOD.

192 ue k(2)/k(3) that characterizes the level of product inhibition scales as ScMnSOD > D. radiodurans Mn

193 ent paper describes the use of steady-state, product inhibition, single-turnover, and kinetic simulat

194 Overall, these results depict a novel product inhibition strategy in which shared substrate an

195 xide dismutase (MnSOD) is characterized by a product inhibition stronger than that observed in bacter

196 Product inhibition studies also support a sequential ord

197 The results of product inhibition studies and isotope effects suggest t

198 rates and products bind the free enzyme, and product inhibition studies are consistent with a random

199 Product inhibition studies demonstrate that CoA binds co

200 pyrophosphorolysis of IMP, GMP, and XMP and product inhibition studies have been used to elucidate t

201 Product inhibition studies have led to a proposed uni-bi

202 Substrate binding and product inhibition studies helped to further elucidate t

203 Product inhibition studies indicate that ADP is competit

204 e for fructose 1, 6-bisphosphate 16-fold and product inhibition studies indicate that this effect is

205 Analysis of product inhibition studies indicated that dCyd kinase fo

206 locity plots with other phosphate donors and product inhibition studies indicated that dCyd kinase fo

207 Initial velocity and product inhibition studies indicated that LdAPRT follows

208 Product inhibition studies indicated that the forward re

209 The minimum kinetic model from the data in product inhibition studies is an ordered bi-bi mechanism

210 Product inhibition studies resulted in two competitive a

211 Steady-state kinetic and product inhibition studies revealed that PP2Calpha emplo

212 Product inhibition studies revealed that the product CoA

213 Steady-state kinetic and product inhibition studies showed that SMYD2 operates vi

214 Product inhibition studies suggest that ADP is the last

215 sed and purified GmHSD, initial velocity and product inhibition studies support an ordered bi bi kine

216 However, product inhibition studies were only consistent with the

217 thionine (AdoMet) as variable substrates and product inhibition studies with methylated DNA and S-ade

218 Product inhibition studies with PP(i) and DGPC demonstra

219 ide and S-adenosyl-L-methionine (AdoMet) and product inhibition studies with S-adenosyl-L-homocystein

220 Product inhibition studies with SUV39H1 showed that S-ad

221 combination of bisubstrate kinetic analysis, product inhibition studies, and dead-end competitive inh

222 acterization, utilizing initial velocity and product inhibition studies, found the mechanism of PPCS

223 In product inhibition studies, thymidine inhibited noncompe

224 On the basis of product inhibition studies, we propose that the reaction

225 estigated using initial velocity methods and product inhibition studies.

226 lt contradicts previous conclusions based on product inhibition studies.

227 velocity analysis and substrate analogue and product inhibition studies.

228 Pong Bi Bi mechanism, which was verified by product inhibition studies.

229 Investigation of the kinetic mechanism using product inhibition suggested that a compulsory-ordered t

230 Theoretical analysis of product inhibition suggested that the inhibition strengt

231 The newly detected concerted substrate and product inhibition suggests that TryS activity is tightl

232 SOD exhibits a substantially higher level of product inhibition than the MnSODs from bacteria.

233 The use of dead-end and product inhibition, the solvent isotope effect, and the

234 sing enzyme concentration, strongly supports product inhibition through hexamer stabilization.

235 Nevertheless, AlkA exhibits significant product inhibition under multiple-turnover conditions, a

236 SPN was unaffected by product inhibition using nicotinamide, suggesting that t

237 mmalian GTP cyclohydrolase is subject to end-product inhibition via an associated regulatory protein

238 HyH product inhibition was observed with emodin analogues, r

239 Moreover, the mechanism of this weakened product inhibition was similar to that in E. coli MnSOD,

240 vide molecular level insights into cellulase product inhibition, we examine the impact of product bin

241 nt enzyme was 2.7-fold more sensitive to CTP product inhibition when compared with the phosphorylated

242 Manganese- and iron-containing SOD exhibit product inhibition whereas Cu/ZnSOD does not.

243 MPG plays a critical role in overcoming the product inhibition, which is achieved by reducing the di

244 ng constants for cellulases and suggest that product inhibition will vary significantly based on the

245 However, this mutant exhibited a strong product inhibition with a zero-order region of superoxid

246 d CTP synthetases were less sensitive to CTP product inhibition with inhibitor constants for CTP of 8