ライフサイエンスコーパス: proenkephalin

1 esults in production of (Met)enkephalin from proenkephalin.

2 ar to amphibian proenkephalin than mammalian proenkephalin.

3 lin and Leu-enkephalin as seen for mammalian proenkephalin.

4 luorogenic peptides, and for recombinant rat proenkephalin.

5 to block known initial cleavage sites within proenkephalin.

6 For proenkephalin, 75 and 96% yields were obtained for the o

7 peak expression levels with substance P and proenkephalin A (218-228).

8 ed with expression levels of substance P and proenkephalin A (amino acids 218-228), respectively, wit

9 Proenkephalin A (PENK) and its receptors are widely dist

10 The precursor neuropeptides proenkephalin A (PENK-A) and protachykinin (PTA) are mar

11 e-amino acid opioid peptide derived from the proenkephalin A family.

12 M8-22), a proteolytically cleaved product of proenkephalin A, is a potent activator of Mas-related G-

13 High levels of proenkephalin-A (pro-ENK) have been associated with decr

14 Proenkephalin-A and mu-opioid receptor mRNA expression i

15 ereas in pregnant rats finasteride decreased proenkephalin-A mRNA expression in the NTS.

16 es have been implicated in the processing of proenkephalin and other neuropeptide precursors.

17 be involved in the proteolytic processing of proenkephalin and perhaps other precursors into active n

18 ed peptides, including some originating from proenkephalin and phosphatidylethanolamine binding prote

19 ake resulted in mRNA levels of proDynorphin, proEnkephalin and POMC, and Dynorphin A1-17 levels that

20 /Sucrose reduced mRNA levels of proDynorpin, proEnkephalin and POMC, and Dynorphin A1-17 levels, rela

21 diet decreases mRNA levels of proDynorphin, proEnkephalin and POMC, as well as levels of Dynorphin A

22 Proteolytic processing of proenkephalin and proneuropeptides is required for the p

23 hy demonstrated that PC2 can directly cleave proenkephalin and that the generation of small opioid pe

24 ta, opioid receptor and proopiomelanocortin, proenkephalin, and prodynorphin transcript levels in cor

25 MP-dependent protein kinase, iNOS, beta-NGF, proenkephalin B and orphanin, corticotrophin-releasing f

26 This proenkephalin-beta-galactosidase transgene has been demo

27 Not only does human skin express proenkephalin, but this expression is upregulated by str

28 In vitro processing of proenkephalin by cathepsin V occurs at dibasic residue s

29 However, the cloning of a full-length proenkephalin cDNA from the CNS of the Australian lungfi

30 ence was detected in the Australian lungfish proenkephalin cDNA.

31 es simplex virus vector that expresses human proenkephalin could be used to attenuate nociception in

32 Also, ECE-2 processes proenkephalin-derived bovine adrenal medulla peptides, a

33 tested the mutated enzymes against a set of proenkephalin-derived substrates, as well as substrates

34 fibers were similar in animals infected with proenkephalin-encoding and beta-galactosidase-encoding v

35 d or eliminated in animals infected with the proenkephalin-encoding virus for at least 7 weeks postin

36 ynorphin-expressing (Pdyn-expressing) and of proenkephalin-expressing (Penk-expressing) medium spiny

37 d receptors, little is known about cutaneous proenkephalin expression, its environmental regulation,

38 A screen for neuroactivity of novel proenkephalin fragments that were found was performed by

39 The proenkephalin gene and protein were expressed in skin an

40 eceptor (TLR)4, and TLR2 agonists stimulated proenkephalin gene expression in melanocytes and keratin

41 Proper transcriptional regulation of the proenkephalin gene requires a switch between distinct fa

42 We disrupted the pre-proenkephalin gene using homologous recombination in emb

43 The rat and mouse proenkephalin genes are selectively expressed from an al

44 In this study, the minimal rat proenkephalin germ line promoter was localized to a 116-

45 diated processing of proopiomelanocortin and proenkephalin; however, similarly to 7B2, proSAAS expres

46 study was to assess regulated expression of proenkephalin in normal and pathological skin and in iso

47 yed to analyze the fate of mutant and native proenkephalins in stably transfected AtT-20 cells.

48 Studies of in vivo cleavage of proenkephalin, in vivo production of alpha-MSH from proo

49 peptide 22), a peptide agonist derived from proenkephalin, inhibited high (but not low) voltage-acti

50 , we studied palatable liquid consumption in proenkephalin knockout (PENK KO) and beta-endorphin-defi

51 s a duplication of either a prodynorphin- or proenkephalin-like gene.

52 atial expression patterns of substance P and proenkephalin, marker neuropeptides of two distinct stri

53 halins were more fully processed than native proenkephalin may provide a route for more efficient pro

54 ted that the early slow processing of mutant proenkephalins may be due to delays in intracellular tra

55 e was a 5-fold increase in the expression of proenkephalin mRNA (502.8 +/- 142% vs. 100 +/- 17.5%, P

56 The long-term expression of the proenkephalin mRNA and its peptides in the kainate-treat

57 he decline in GAD67 mRNA (25%, P < 0.01) and proenkephalin mRNA levels (35%, P < 0.01).

58 here were no differences in proDynorphin and proEnkephalin mRNA levels in the ARC (0.05).

59 nervous systems, the neuropeptide precursor proenkephalin must be endoproteolytically cleaved by enz

60 a Leu-enkephalin-coding gene, distinct from proenkephalin, must be expressed in lungfish.

61 hat yielded this opioid sequence in tetrapod proenkephalin occurred at some point in the radiation of

62 h native proenkephalin, processing of mutant proenkephalins occurred more slowly at early stages and

63 ), but did not affect arcuate mRNA levels of proEnkephalin or proOpiomelanocortin (POMC), or PVN leve

64 Met)enkephalin peptide neurotransmitter from proenkephalin (PE) in the regulated secretory pathway of

65 Proenkephalin (PE) is a prohormone containing dibasic si

66 Proenkephalin (PE) represents the precursor protein of t

67 However, a causal link between proenkephalin (Penk) expression and vulnerability to her

68 In adult hamsters, basal proenkephalin (Penk) gene expression in adrenals is inde

69 e hydroxylase (TH), prodynorphin (PDYN), and proenkephalin (PENK) genes contain cAMP response element

70 search was to assess the prognostic value of proenkephalin (PENK) levels in acute myocardial infarcti

71 These genes included prodynorphin (PDYN) and proenkephalin (PENK), among others.

72 mined the response of CRH or c-fos mRNAs and proenkephalin (PPE) mRNA and heteronuclear RNA (hnRNA, p

73 iosynthesis and the neuropeptide transmitter proenkephalin (ppEnk) in butyrate-differentiated PC12 ce

74 opioid peptides that are derived from a pre-proenkephalin precursor protein.

75 rable to mammals suggested that the lungfish proenkephalin precursor should contain the sequences of

76 Expression of proenkephalin precursors and neuropeptide products in th

77 ganglia (lacZ-containing virus) and of human proenkephalin (preproenkephalin-encoding virus) in the c

78 , surprisingly, overall processing of mutant proenkephalins proceeded efficiently, and alternative si

79 otease" (PTP) has been identified as a major proenkephalin processing enzyme in secretory vesicles of

80 cursor protein (APP), potently inhibited the proenkephalin processing enzyme known as prohormone thio

81 Variations in the extent of proenkephalin processing in vivo suggest involvement of

82 anule prohormone thiol protease (involved in proenkephalin processing).

83 cysteine protease "PTP" that participates in proenkephalin processing.

84 When compared with native proenkephalin, processing of mutant proenkephalins occur

85 YN), but not pro-opiomelanocortin (POMC) nor proEnkephalin (proENK) were significantly decreased 2 h

86 ized model ssDNA regulatory element from the proenkephalin promoter.

87 known secretory pathway proteins, including proenkephalin, proopiomelanocortin, protachykinins A and

88 s including those derived from prodynorphin, proenkephalin, proSAAS, and amyloid precursor protein.

89 t exercise included a number of fragments of proenkephalin, prothyrotropin-releasing hormone, secreto

90 Analysis of proenkephalin reaction products using immunoblotting and

91 transcription factor known to interact with proenkephalin regulatory sequences within the transgene,

92 reporter was driven in PVN neurons by human proenkephalin regulatory sequences.

93 the specificity of recombinant mouse PC2 for proenkephalin-related internally quenched (IQ) peptides,

94 herpes simplex virus-based vector expressing proenkephalin reversed nocisponsive behavioral responses

95 sors, mouse proopiomelanocortin (mPOMC), rat proenkephalin (rPE), and human proghrelin, were used as

96 this precursor is more similar to amphibian proenkephalin than mammalian proenkephalin.

97 otease of chromaffin granules for converting proenkephalin to the active enkephalin peptide neurotran

98 It is demonstrated that expression of the proenkephalin transgene product was up-regulated signifi

99 herpes simplex virus vector expressing human proenkephalin (vector SHPE) or a lacZ-expressing control

100 The processing of proenkephalin was impaired in PC1 KO mouse brains with a

101 The fact that mutant proenkephalins were more fully processed than native pro

102 nkephalin sequence in lungfish and amphibian proenkephalin would suggest that the mutations that yiel