ライフサイエンスコーパス: pronuclear

1 downstream target of the APC, causes similar pronuclear and a-p polarity defects.

2 In this issue of Developmental Cell, using a pronuclear assembly assay, Xue et al. demonstrate that D

3 nopus egg extracts excess microtubules cause pronuclear assembly defects, leading to abnormal morphol

4 f the maternal pronucleus and argues against pronuclear asynchrony as the primary cause of CI lethali

5 This stimulated pronuclear centering, DNA synthesis, and nuclear envelop

6 pronuclear migration, and moreover, a robust pronuclear centration and rotation very similar to that

7 e spatial organization and forces needed for pronuclear centration, rotation, and spindle displacemen

8 itially by the migration and rotation of the pronuclear complex (PNC) and its two associated astral m

9 tween initial centration and rotation of the pronuclear complex and entry into mitosis, and the model

10 simultaneous centration and rotation of the pronuclear complex observed in vivo.

11 ential for nucleus-centrosome attachment and pronuclear congression during fertilization.

12 e, but macroH2A is progressively lost during pronuclear decondensation prior to synkaryogamy.

13 itment and degradation of maternal mRNA, and pronuclear development.

14 in and terminate development after the first pronuclear division.

15 on, and centrosome association with the male pronuclear envelope are defective in dli-1(RNAi) embryos

16 he recruitment of YA or lamin Dm to the male pronuclear envelope, suggesting that the mutations affec

17 vity are incompatible with the presence of a pronuclear envelope.

18 s appear incompatible with the presence of a pronuclear envelope.

19 s MAP kinase remains active, however, and no pronuclear envelopes form.

20 ly with the formation of the male and female pronuclear envelopes.

21 (anchoring nuclei to the exchange junction), pronuclear exchange, pronuclear fusion, and anchoring po

22 Whereas microtubules normally promote sperm pronuclear expansion, in Dppa2-depleted Xenopus egg extr

23 the action potential, sperm entry, and male pronuclear formation can occur in the absence of PLCgamm

24 that PAWP triggers calcium oscillations and pronuclear formation in human and mouse oocytes similar

25 a2+ action potential, sperm entry, and sperm pronuclear formation were not prevented by injection of

26 ulum, an increase in intracellular pH, sperm pronuclear formation, MAP kinase dephosphorylation, and

27 t a precise time and location during nascent pronuclear formation.

28 dc2/cyclin B1 and MAP kinase activities, and pronuclear formation.

29 iated with nuclear structures at the time of pronuclear formation.

30 imately 4,000 genes are transcribed prior to pronuclear fusion and in the one- to four-cell embryos.

31 8 and Y11, inhibitors of FAK, interfere with pronuclear fusion and reduce the abundance of pY(397)FAK

32 emonstrate that Brambleberry is required for pronuclear fusion following fertilization in zebrafish.

33 immediately after fertilization and prior to pronuclear fusion in metazoa, suggesting that newly tran

34 the exchange junction), pronuclear exchange, pronuclear fusion, and anchoring postzygotic nuclear div

35 diately after fertilization in utero, before pronuclear fusion, and before the first cleavage of the

36 decondensation of the sperm chromatin after pronuclear fusion, nuclear envelope breakdown and format

37 At pronuclear fusion, the diploid zygotic nucleus was assoc

38 y transcribed in the A. suum zygote prior to pronuclear fusion.

39 a known defect of klp3A oocytes in mediating pronuclear fusion.

40 development, we generated transgenic mice by pronuclear injection of a 380 kb yeast artificial chromo

41 These mice were generated by pronuclear injection of a bacterial artificial chromosom

42 ng-free method to target the mouse genome by pronuclear injection of a commercial Cas9 protein:crRNA:

43 Transgenic mice were generated by pronuclear injection of a construct carrying a 3-kilobas

44 l for Charcot-Marie-Tooth disease type 1A by pronuclear injection of a YAC containing the human PMP22

45 founder transgenic mice that was produced by pronuclear injection of an SssI-methylated construct cou

46 Pronuclear injection of DRAP and mutant oligonucleotides

47 rthermore, transgenic mice were generated by pronuclear injection of the modified BAC, and germline t

48 stem (ES) cell-based targeted insertion, or pronuclear injection, of the knockdown expression casset

49 ased approach to produce transgenic mice via pronuclear injection, whereby an intact single-copy tran

50 nto the mouse as transgenes via conventional pronuclear injection.

51 gmyc and introduced into the mouse zygote by pronuclear injection.

52 e not generated after two separate rounds of pronuclear injections.

53 as probes to study the formation of the male pronuclear lamina in Drosophila melanogaster.

54 ologically differentiated out of a series of pronuclear masses.

55 Drosophila egg's competence to support male pronuclear maturation is acquired during activation.

56 and H3-K27 and these signals increase during pronuclear maturation.

57 cell stage arrest before entry into mitosis: pronuclear meeting occurred normally, but nuclear envelo

58 The second phase begins after pronuclear meeting, when the sperm asters begin to invad

59 The transposase-enhanced pronuclear microinjection (PNI) technique described here

60 enic mouse production because of the ease of pronuclear microinjection and its overall fecundity.

61 For Cre lines generated via pronuclear microinjection of a Cre transgene construct,

62 Pronuclear microinjection of ZFNs, shown by our data to

63 and reliable transgene expression to classic pronuclear microinjection or somatic cell nuclear transf

64 ransgenic L1 mouse lines are constructed via pronuclear microinjection, a procedure that typically re

65 d reliable transgene expression with classic pronuclear microinjection, and it offers comparable effi

66 d reliable transgene expression with classic pronuclear microinjection, and it offers comparable effi

67 (TetOp)(7)-FasL responder mice, generated by pronuclear microinjection, were bred with Clara cell sec

68 lpha A-crystallin promoter were generated by pronuclear microinjection.

69 development of strategies aimed at bypassing pronuclear microinjection.

70 Transgenic mice were generated by pronuclear microinjection.

71 uires fewer than half the animals needed for pronuclear microinjection.

72 of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis.

73 Pronuclear migration and formation of the first mitotic

74 cate that sperm asters are not essential for pronuclear migration but are required for rapid female p

75 Pronuclear migration does not occur in activated eggs la

76 Meiotic spindles are disorganized, pronuclear migration fails, and the mitotic apparatus fo

77 t before karyogamy in eukaryotes is known as pronuclear migration or as nuclear congression in Saccha

78 Pronuclear migration produces global cytoplasmic flows t

79 Our simulations show pronuclear migration, and moreover, a robust pronuclear

80 Specifically, pronuclear migration, centrosome separation, and centros

81 exhibit defects in chromosome condensation, pronuclear migration, kinetochore assembly, and spindle

82 ditis elegans to identify genes required for pronuclear migration, one of the first events in embryog

83 ature show defects in centrosome separation, pronuclear migration, rotation of the centrosome/nucleus

84 key consequences of HIs for the mechanics of pronuclear migration.

85 clear envelope (NE) constituent required for pronuclear migration.

86 pronucleus-centrosome attachment and female pronuclear migration.

87 Arp2/3 mutants correlates with reduced male pronuclear migration.

88 e male pronucleus and the centrosomes during pronuclear migration.

89 metaphase II (MII) arrest and progressed to pronuclear, MIII, and anaphase/telophase III stages.

90 of cytoplasmically driven microtubule-based pronuclear motion in the single-celled Caenorhabditis el

91 migration but are required for rapid female pronuclear movement and premitotic positioning of the pr

92 ygotes progress into interphase and commence pronuclear (PN) formation.

93 ication is the male pronucleus (e.g., DNA or pronuclear proteins) or some extranuclear factor from th

94 Male pronuclear remodeling in the injected oocytes was requir

95 f possible functions of the KLP3A protein in pronuclear specification and migration.

96 ost Setd1b(Gdf9) cKO zygotes remained in the pronuclear stage and displayed polyspermy in the perivit

97 embryo is likely determined as early as the pronuclear stage and may be predicted by a 12-gene trans

98 ere microinjected into genetically identical pronuclear stage embryos.

99 owing fertilization and progression from the pronuclear stage into mitosis.

100 erleukin-2 receptor subunit gamma (IL2RG) in pronuclear stage marmoset embryos.

101 monkey oocytes, significantly upregulated in pronuclear stage zygotes, diminished in early cleaving e

102 At the pronuclear stage, activated Src-family PTKs became conce

103 ior to fertilization and are arrested at the pronuclear stage.

104 ply after fertilization, as the cytoplasm of pronuclear-stage zygotes is reportedly inactive.

105 Here, we show that in advanced pronuclear-stage zygotes the paternal pronucleus contain

106 Maternal pronuclear transfer and CTCF mRNA microinjection experim

107 We believe that pronuclear transfer between zygotes, as well as the rece

108 en generations by maternal spindle transfer, pronuclear transfer or polar body transfer: all involve

109 sfer, including spindle-chromosome transfer, pronuclear transfer, and first and second polar body tra

110 e we report the first preclinical studies on pronuclear transplantation (PNT).

111 Couples with high SPTRX3 produced fewer two-pronuclear zygotes and had a reduced pregnancy rate (19.