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1 e protein that promotes the translocation of propilin.
2 sed of TraA pilin subunits derived from TraA propilin.
3  An in vitro propilin cleavage assay yielded propilin (13 kDa), the pilin polypeptide (7 kDa), and a
4 vage of a 51-amino-acid leader sequence from propilin and require LepB and TraQ but not the SecA-SecY
5 TraQ is not necessary for the proteolysis of propilin and therefore is likely to act as a chaperone-l
6 ously demonstrated to encode the full-length propilin, and other genes of this operon have been impli
7  identity since the only proline residues of propilin are found within the leader peptide.
8  signal peptidase cleavage sequence of VirB2 propilin cause the formation of aberrant T pilin and als
9        The potential role of the exact VirB2 propilin cleavage and cyclization in T pilus biogenesis
10                                  An in vitro propilin cleavage assay yielded propilin (13 kDa), the p
11                                            A propilin derivative with an amino-terminal signal sequen
12                              We propose that propilin insertion in the plasma membrane proceeds indep
13    Using propilin processing as a measure of propilin insertion into the plasma membrane, we found th
14        These results present a model whereby propilin insertion leads to processing by leader peptida
15         Maturation of the F-pilin precursor, propilin, involves three F plasmid transfer products: Tr
16                                          The propilin is processed by signal sequence cleavage and co
17                                        VirB2 propilin is processed by the removal of a 47-amino-acid
18 ounterpart of TraA is VirB2, which like TraA propilin is processed into a 7.2-kDa product that compri
19                                  The unusual propilin leader peptide and the dependence of its cleava
20        Thus, despite its unusual length, the propilin leader peptide seems to perform a function simi
21 is responsible for the removal of the entire propilin leader peptide.
22 mine the involvement of host cell factors in propilin maturation.
23 r depletion of SecA and SecY does not affect propilin maturation.
24 three F plasmid transfer products: TraA, the propilin precursor; TraQ, which promotes efficient propi
25                                        Using propilin processing as a measure of propilin insertion i
26 ature in a LepBts (leader peptidase B) host, propilin processing was inhibited.
27 otive force did cause a marked inhibition of propilin processing, indicating that membrane insertion
28 in precursor; TraQ, which promotes efficient propilin processing; and TraX, which is required for ace
29                     The in vitro and in vivo propilin-processing reactions proceed similarly in a sin
30 re pilin begins at amino acid 52 of the TraA propilin sequence.
31  in A. tumefaciens and the processing of the propilin TraA of the F plasmid now extends to E. coli.
32 vides a compelling argument for VirB2 as the propilin whose 7.2-kDa processed product is the pilin su
33  the other hand, a chimeric protein with the propilin wild-type leader peptide fused to the mature po

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