ライフサイエンスコーパス: protamine

1 calcium or the polycationic heparin antidote protamine.

2 , and activated platelets in the presence of protamine.

3 quickly reverse the effects of heparin using protamine.

4 t activity without the lung injury seen with protamine.

5 nd Escherichia coli to become susceptible to protamine.

6 ticoagulant polyion heparin and its antidote protamine.

7 polyion sensors for the detection of polyion protamine.

8 e strong binding reaction of polyanions with protamine.

9 er separation from CPB and administration of protamine.

10 ished promptly after heparin antagonism with protamine.

11 ible electrochemical sensors for the polyion protamine.

12 he determination of the polyions heparin and protamine.

13 ch is close to the excess positive charge of protamine.

14 do not produce as uniform structures as bull protamine.

15 s, and was 4- to 20-fold more effective than protamine.

16 Hs are rapidly and completely neutralized by protamine.

17 selective and sensitive for the detection of protamine.

18 nation of trace amount of an important drug, protamine.

19 ntial based on cation-exchange extraction of protamine.

20 gulation: citrate and calcium or heparin and protamine.

21 sible, selective, and sensitive detection of protamine.

22 aboratory from enzymatic digestion of native protamine.

23 omatin compacted primarily by sperm-specific protamines.

24 d by transition proteins and subsequently by protamines.

25 fore eventual replacement of histones by the protamines.

26 f most vertebrate sperm cells is packaged by protamines.

27 is achieved by replacement of histones with protamines.

28 lation and replacement of most histones with protamines.

29 n of transition nuclear protein 1 (Tnp1) and protamine 1 (Prm1) genes, the products of which are requ

30 nes Tnp1 (transition protein 1), Tnp2, Prm1 (protamine 1), and Prm2, all of which are essential for c

31 Our data demonstrate that, like protamine-1 and -2, both alleles of Klhl10 are required

32 expression of Transition protein 2 (TP2) and Protamine 2 (Prm2) proteins (chromatin remodelers, essen

33 , NLP, and Nph share roles in the removal of protamine A.

34 Ex vivo and in vivo, protamine abolishes well-known apelin effects, such as a

35 id decrease in platelet count directly after protamine administration, we determined the incidence an

36 doped polymeric membranes but also to reveal protamine adsorption at the membrane/water interface.

37 Protamine adsorption is thermodynamically more favorable

38 only recently recognized immune response to protamine after cardiopulmonary bypass (CPB) surgery wit

39 ne, arginine homopolymers (R2, R6, R10), and protamine, all of which can potentially transport across

40 Compared to protamine, all the LMWP peptides showed lower DNA bindin

41 tamine and heparin were present but not with protamine alone.

42 od and Drug Administration-approved compound protamine-already used clinically after cardiac surgery-

43 ivity, which confers bacterial resistance to protamine and alpha-helical CAMPs.

44 A and amiC, elevated bacterial resistance to protamine and alpha-helical peptides magainin 2 and meli

45 orption reactions of the natural polypeptide protamine and also for Ca(2+) and Mg(2+) transfers facil

46 ption on microchannel surfaces modified with protamine and biotin, respectively, whereas bovine serum

47 ically relevant polyions such as heparin and protamine and drastically improve the sensitivity of ion

48 e real-time detection of injected samples of protamine and heparin at up to 20 samples/h.

49 We hypothesized that patients exposed to protamine and heparin during CPB will develop antibodies

50 ntibodies induced thrombocytopenia only when protamine and heparin were present but not with protamin

51 on mixed potential responses, for which both protamine and Na(+) need to be transferred simultaneousl

52 in and alpha-chymotrypsin are detected using protamine and synthetic polycationic oligopeptides that

53 Protamine and the R10 decamer, especially, induce the la

54 GLD2 also affects the incorporation of protamines and the stability of dynamin and transition p

55 duction of intermolecular disulfide bonds in protamines and their eviction from sperm during fertiliz

56 y complete elimination of those encoding the protamines and transition proteins, but was not associat

57 ration-free methodology for the detection of protamine (and, by titration, heparin) in undiluted huma

58 A divalent cation (calcium), a polyion (protamine), and an anion (chloride) were successfully de

59 in nucleosomes and histone variants (and not protamine), and we find linker histones high and H4K16ac

60 (trypsin) based on proteolytic digestion of protamine, and polyanions (pentosan polysulfate and hepa

61 Remarkably, protamine antagonist activity is fully reversed by hepar

62 ross-reactivity toward the mice-derived anti-protamine antibodies.

63 We designed a protamine-antibody fusion protein to deliver siRNA to HI

64 events in the group assigned to heparin and protamine anticoagulation (11 vs 2; p = 0.011).

65 cuit life compared with regional heparin and protamine anticoagulation, does not affect cytokine leve

66 on cytokine levels compared with heparin and protamine anticoagulation.

67 ous polyamines and the heterogeneous protein protamine are quite similar, demonstrating the universal

68 Protamines are arginine-rich IDPs involved in the conden

69 to sperm chromatin remodeling, during which protamines are expelled and replaced by histones.

70 Protamines are small, highly positively charged peptides

71 Protamines are synthesized during spermiogenesis and con

72 om histone H2A has been developed to replace protamine as a conditionally reversible, nucleic acid co

73 itro and in vivo than the NPs assembled with protamine as the nucleic acid condensing agent.

74 Reversible adsorption of protamine at low concentrations down to 0.038 mug/mL is

75 Adsorptive preconcentration of protamine at the membrane/water interface is quantitativ

76 32 is required for the removal of Drosophila protamine B in vitro, whereas NAP-1, NLP, and Nph share

77 ly development to facilitate the switch from protamine-based sperm chromatin structures to the somati

78 ntly irreversible potentiometric response to protamine because back-extraction of protamine from the

79 Conversely, protamine binds to the fibrin clot, which could explain

80 demonstrate a new pharmacologic property of protamine-blockade of APJ-that could explain some advers

81 ased resistance to the antimicrobial peptide protamine both in ompT mutants and in wild-type E. coli

82 is exhibited hypersusceptibility not only to protamine but also to alpha-helical cathelicidin LL-37 a

83 colorimetric response to polycations such as protamine but not to small inorganic cations because onl

84 s reduce arthritis by 19%, our anti-C5aR1 Ab-protamine-C5 siRNA conjugate was effective in reducing a

85 We then generated a novel anti-C5aR1 Ab-protamine-C5 siRNA conjugate.

86 Protamine calibration curves were recorded at physiologi

87 can inhibit their oxidase-like activity and protamine can recognize heparin, we prepared the protami

88 Protamine chaperone TAP/p32 dissociates DNA-protamine co

89 Here we identify four Drosophila protamine chaperones that mediate the dissociation of pr

90 just before histone-to-transition protein-to-protamine chromatin remodelling in spermiogenesis.

91 hd5) as a master regulator of the histone-to-protamine chromatin remodelling process.

92 harboring a single copy insert of the human protamine cluster were subjected to Micrococcal Nuclease

93 usion protein (F105-P) was designed with the protamine coding sequence linked to the C terminus of th

94 ng the condensation and stability of the DNA-protamine complex prior to the formation of inter-protam

95 Protamine chaperone TAP/p32 dissociates DNA-protamine complexes in vitro only when protamine oligome

96 These materials include covalent conjugates, protamine complexes, nanoparticles based on lipids or po

97 was continuously monitored by measuring the protamine concentration as it is cleaved by enzyme into

98 In this method with increasing the protamine concentration, the fluorescence of the quantum

99 xhibited a stable and reversible response to protamine concentrations ranging from 0.05 to 30 mg L-1.

100 eparin followed by complexation with albumin-protamine conjugate, termed 'camouflage'.

101 arin complexes, but were cross-reactive with protamine-containing insulin preparations.

102 l biosensor, under the optimized conditions, protamine could be measured in the range of 2.0-200 ng m

103 erved that the neutral amino acids in salmon protamine decrease the net attraction between protamine-

104 e, an ErbB2 single-chain antibody fused with protamine delivered siRNAs specifically into ErbB2-expre

105 fluence of common interfering species on the protamine detection was studied.

106 With the diffusion coefficient of protamine determined to be (1.2 +/- 0.1) x 10(-6) cm(2)/

107 Protamine displays a 390-nM affinity for APJ and behaves

108 mine complex prior to the formation of inter-protamine disulfide cross-links.

109 osphoramidate-dipropylamine) (PPA) and Lipid-Protamine-DNA (LPD) nanoparticles consistently showed th

110 derstand the nature of the forces condensing protamine-DNA assemblies and their dependence on amino a

111 chaperones that mediate the dissociation of protamine-DNA complexes: NAP-1, NLP, and nucleophosmin a

112 rotamine decrease the net attraction between protamine-DNA helices compared with the equivalent homo-

113 bservations have biological implications for protamine-DNA packaging in sperm heads.

114 A model is also presented for the bull protamine.DNA complex in native sperm cell chromatin tha

115 rgo to the cytoplasm than our previous lipid/protamine/DNA (LPD) formulation, leading to a significan

116 ansfer conditions controlled by diffusion of protamine, DNNS, and the complex in the outer solution o

117 .005, 0.89 +/- 0.01, and 0.065 +/- 0.008 for protamine, DNNS, and the complex, respectively.

118 ine(n+) (aq) right harpoon over left harpoon protamine-DNNS complex (DCE).

119 the MARs bounding the PRM1 --> PRM2 --> TNP2 protamine domain have many and varied functions.

120 potentiation an approximately 13.7-kb mouse protamine domain of increased nuclease sensitivity flank

121 x attachment regions (MARs) encompassing the protamine domain were created.

122 the multigenic PRM1 --> PRM2 --> TNP2 human protamine domain.

123 by transition proteins, and subsequently by protamines during spermiogenesis.

124 reveal its role in regulating the histone-to-protamine exchange during spermiogenesis.

125 ross chromatin remodeling that occurs during protamine exchange.

126 in-associated bleeding in surgical settings, protamine, exhibits adverse effects.

127 ion is thermodynamically more favorable than protamine extraction as shown by cyclic voltammetry at p

128 ion-transfer voltammetry not only to confirm protamine extraction into ionophore-doped polymeric memb

129 The voltammetrically reversible protamine extraction results in an apparently irreversib

130 otentiometric protamine response is based on protamine extraction.

131 d to study the phase transfer of polypeptide protamine facilitated by complexation with charged ionop

132 fide bonds hold the terminal domains of bull protamine folded back onto the central DNA binding domai

133 Based on available evidence, the use of protamine following CEA is associated with a reduction i

134 leic acid therapeutics, which is captured by protamine for siRNA delivery.

135 low immunogenicity, they would be safer than protamine for use in gene therapies.

136 After fertilisation, sperm DNA exchanges protamines for histones recruited from oocyte cytoplasm,

137 of sperm chromatin remodeling that exchanges protamines for histones to form the nucleosome-based chr

138 fertilization, the paternal genome exchanges protamines for histones, undergoes DNA demethylation, an

139 copy and light scattering, we show that bull protamine forms particles with DNA that are morphologica

140 nzymatically to produce low molecular weight protamine fragments (LMWPs) of various lengths and amino

141 onse to protamine because back-extraction of protamine from the membrane extremely slows down at the

142 Cation-exchange extraction of polypeptide protamine from water into an ionophore-based polymeric m

143 Protamines from eutherian mammals, including bulls and h

144 ondary structure is also critical for proper protamine function.

145 We show here that antibody-protamine fusion proteins targeting the human integrin l

146 s and reveals transition nuclear protein and protamine genes as direct targets of JHDM2A.

147 rs (95% CI, 13.3-34.0 hr) in the heparin and protamine group (log rank p = 0.0037, 204 circuits).

148 e serum (group 2), StemPro medium (group 3), protamine (group 4), and protamine plus heparin (group 5

149 lows the following sequence: R10 (decamer) > protamine > R6 (hexamer) > L-arginine.

150 h long-acting (glargine, detemir) or neutral protamine Hagedorn (NPH) insulin between 2002 and 2012,

151 nsulin product than the conventional neutral protamine Hagedorn (NPH) insulin for diabetic control.

152 strong rationale favoring glargine, neutral protamine Hagedorn insulin, insulin detemir, or fixed-ra

153 sodes of nighttime hypoglycemia than neutral protamine Hagedorn regimens.

154 ween insulin type (basal, pre-mix or Neutral Protamine Hagedorn, NPH) while ANCOVAs compared haemoglo

155 ion-selective electrodes for the polycation protamine have been reported, for instance, a pulsed chr

156 rmolecular disulfide bonds between DNA-bound protamines help stabilize the chromatin of mature mammal

157 surgery, 57 (9.6%) tested positive for anti-protamine-heparin antibodies at baseline and 154 (26.6%)

158 Seven patients had platelet-activating, anti-protamine-heparin antibodies at baseline and showed a gr

159 Platelet-activating anti-protamine-heparin antibodies show several similarities w

160 as a risk factor for the development of anti-protamine-heparin antibodies.

161 ical biomolecules to build up a thin film of protamine-heparin complex on Kapton, but also the first

162 during CPB will develop antibodies (Abs) to protamine/heparin (PRT/H) complexes that are capable of

163 of catalase layers is compared with that of protamine, horseradish peroxidase, and inactivated catal

164 We observed that the ferumoxytol-heparin-protamine (HPF) nanocomplexes were stable in serum-free

165 The primary structure of mammalian protamine I can be divided into three domains, a central

166 Optical determination of protamine in human blood plasma using the exhaustive nan

167 Because nucleosomes are widely replaced by protamine in mature human sperm, the epigenetic contribu

168 s successfully used for the determination of protamine in real samples.

169 monstrated to be useful for the detection of protamine in the therapeutic range of undiluted human bl

170 ient selectivity for the direct detection of protamine in undiluted whole blood samples.

171 erized conformational ensembles for a set of protamines in aqueous milieus using molecular simulation

172 DA)-approved drugs--ferumoxytol, heparin and protamine--in serum-free medium to form self-assembling

173 rm cell chromatin reorganization and reduced protamine incorporation.

174 ckdown podocytes were also protected against protamine-induced injury.

175 te the prodrug feature, and subsequently the protamine-induced reversal of heparin inhibition to resu

176 to the fibrin clot, which could explain how protamine instigates clot lysis and increases bleeding a

177 The stoichiometry for protamine interaction with ODSH is determined to average

178 Protamine is a natural DNA condensation agent and has be

179 a potentiometric super-Nernstian response to protamine is also observed.

180 Protamine is an antagonist of apelin receptor, and its a

181 The polyion protamine is detected in 10-fold diluted blood samples i

182 The amino acid content alone of salmon protamine is enough to rationalize the forces that packa

183 Protamine is routinely used to reverse heparin anticoagu

184 A striking and general feature of protamines is the almost exclusive use of arginine over

185 ies compared to salmon nuclei despite salmon protamine lacking cysteine residues.

186 Cysteine oxidation in protamines leads to their oligomerization and contribute

187 improvement of the sensor sensitivity to low protamine levels.

188 Low molecular weight protamine (LMWP) is a peptide fragment produced in our l

189 e naturally derived CPP low-molecular-weight protamine (LMWP) to modify PLGA NP for enhanced drug del

190 after conjugation with low molecular weight protamine (LMWP), a cell penetrating peptide (CPP), insu

191 mbination methods, with low molecular weight protamine (LMWP), a cell-penetrating peptide (CPP) which

192 P) developed in our lab-low molecular weight protamine (LMWP).

193 igonucleotide (ASO) and low-molecular weight protamine (LMWP).

194 Short arginine-rich proteins called protamines mediate the near crystalline DNA packaging in

195 th ODSH is determined to average 1.39 microg protamine/microg ODSH in plasma.

196 amine can recognize heparin, we prepared the protamine-modified Pt NPs through direct adsorption on t

197 m(2)/s, the ionic charge transferred by each protamine molecule was obtained as +20 +/- 1, which is c

198 Protamine molecules bind to and condense DNA in the sper

199 voltammograms are based on desorption of all protamine molecules that are transferred across the inte

200 olecular disulfide bonds formed between bull protamine molecules within in vitro DNA condensates is c

201 ane (DCE) interfaces, i.e., sDNNS(-) (DCE) + protamine(n+) (aq) right harpoon over left harpoon prota

202 Both charge number of transferred protamine, n, and complexation stoichiometry, s, were de

203 o that obtained by using Ferumoxytol-heparin-protamine nanocomplex; and (ii) cells can be re-sized to

204 Although the cross-linked protamine network is known to stabilize the resulting nu

205 s DNA-protamine complexes in vitro only when protamine oligomers are first converted to monomers by D

206 s the direct injection and online removal of protamine-oligonucleotide nanoparticles ("proticles") wi

207 rface probes the clinical heparin antagonist protamine or the physiological partner antithrombin III

208 ns are further substituted by sperm-specific protamines, P1 and P2, to form a highly condensed sperm

209 o medium (group 3), protamine (group 4), and protamine plus heparin (group 5).

210 anscription of Transition proteins (Tnp) and Protamines (Prm), exhibiting chromatin compaction defect

211 he chromatin domain containing the two human protamines PRM1 and PRM2 and the transition protein TNP2

212 ssion of transition proteins (Tnp1/Tnp2) and protamines (Prm1/2).

213 ted 2 unrelated positively charged proteins, protamine (PRT) and lysozyme, and studied H-dependent in

214 ined the incidence and clinical relevance of protamine-reactive antibodies in patients undergoing car

215 In summary, we identified factors mediating protamine removal from DNA and reconstituted in a define

216 e nascent male pronucleus, the machinery for protamine removal remains largely unknown.

217 d5 as a multi-faceted mediator of histone-to-protamine replacement and depict the cascade of molecula

218 ed in genomic regions that escape histone to protamine replacement in human and mouse sperm.

219 on to adsorption, voltammetric extraction of protamine requires approximately 0.2 V more negative pot

220 ation of the CpxR/CpxA system can facilitate protamine resistance because nlpE overexpression elevate

221 s agreement confirms that the potentiometric protamine response is based on protamine extraction.

222 e intermolecular sulfur-sulfur bonds of bull protamine results in tighter DNA packing.

223 Systemic delivery of lipid-protamine-RNA nanoparticles encapsulating a chemically m

224 sample (5.8 muL) confined between a tubular protamine selective membrane (inner diameter, 600 mum) a

225 containing dinonylnaphthalenesulfonate as a protamine-selective ionophore.

226 The protamine-selective solid-contact sensor was fabricated

227 trypsin digestion are not detectable by the protamine-sensing membrane.

228 The protamine-sensing optode platform is used to indirectly

229 A protamine-sensitive mutant identified carried the transp

230 SH in buffer and plasma is described using a protamine-sensitive polymer membrane electrode as the de

231 Here, such protamine-sensitive pulstrodes are applied for the real-

232 The protamine sensors exhibited a stable and reversible resp

233 electrostatic attraction between heparin and protamine-stabilized Pt NPs induced nanoparticle aggrega

234 insight into its potential to be a clinical protamine substitute as well as a non-toxic cell penetra

235 before study agent (sample 1), 10 mins after protamine sulfate administration after cardiopulmonary b

236 In a typical case, coassembly of protamine sulfate and perylene dye via electrostatic att

237 The effects of protamine sulfate and vanadate on Nephrin phosphorylatio

238 sulated with pancreatic islet cells by using protamine sulfate as a clinical-grade alginate cross lin

239 Protamine sulfate can be administered at the conclusion

240 s and other mammalian cells with ferumoxides-protamine sulfate complexes (FE-Pro), cellular toxicity,

241 n subjected to podocyte injury in vivo using protamine sulfate or nephrotoxic serum (NTS).

242 In a mouse model of podocyte injury, protamine sulfate perfusion of the Cfl1 mutant mouse ind

243 e podocyte foot process effacement following protamine sulfate perfusion.

244 Administration of protamine sulfate rapidly damaged the isolated glomeruli

245 ons in this model using high-dose heparin or protamine sulfate support the pathogenic role of surface

246 AC133(+) cells labeled with ferumoxide-protamine sulfate were mixed with either rat glioma or h

247 ated SPIO used as an MRI contrast agent, and protamine sulfate, conventionally used to reverse hepari

248 l facet cell QIRs with the cationic protein, protamine sulfate, led to epithelial exfoliation and era

249 infusion of the positively charged protein, protamine sulfate, the reverse was observed with mPF4(+/

250 Cationic protamine sulfate, which forms similar complexes with he

251 d chelation of extracellular calcium reduced protamine sulfate-induced damage, suggesting that calciu

252 eficient mice display impaired recovery from protamine sulfate-induced foot process effacement and li

253 On the contrary, protamine sulfate-induced phosphorylation at Tyr-1176/11

254 npo(-/-) mice display impaired recovery from protamine sulfate-induced podocyte foot process (FP) eff

255 the cathepsin L inhibitor E64 all inhibited protamine sulfate-mediated barrier changes, which sugges

256 e coagulation assays, and reversibility with protamine sulfate.

257 f B. anthracis and screened them for altered protamine susceptibility.

258 remixed biaspart insulin (70% insulin aspart protamine suspension and 30% insulin aspart of rDNA orig

259 The importance of the bull protamine terminal domains in controlling the bull sperm

260 e positions of the cysteine residues in bull protamine that form intermolecular disulfide bonds.

261 , ascorbic acid 6-palmitate and salmon sperm protamine, that effectively inhibited anthrax cytotoxic

262 In the presence of a given level of protamine the initial rate of reaction can be linearly r

263 rin concentration in whole blood samples via protamine titration are demonstrated.

264 lity of the technique for monitoring heparin/protamine titrations in physiological saline solutions i

265 Heparin-protamine titrations were performed in undiluted human b

266 oposed model for binding of polyarginine and protamine to DNA provides a convenient framework for und

267 ellulose acetate filter membrane coated with protamine to eliminate addition of the indicator polycat

268 Heparin interacts with protamine to form ultralarge complexes that are immunoge

269 The addition of protamine to the medium or treatment of the cells with c

270 then reacted with either HIV-Tat peptide or protamine to yield a nanoparticle with membrane-transloc

271 matin histones are replaced by arginine-rich protamines to densely compact DNA in sperm heads.

272 on of the replacement of somatic histones by protamines to epigenetic control of gene transcription.

273 ch histones are replaced with sperm-specific protamines to repackage the genome into the highly compa

274 asaccharide Arixtra and polycationic peptide protamine, to yield the membrane permeability that is lo

275 This approach linked robust endogenous protamine transcription and transgene suppression to its

276 to quantitatively and mechanistically assess protamine transfer at ionophore-based polymeric membrane

277 an irreversible transient CV of facilitated protamine transfer gives an apparent k(0) value of 3.5 x

278 ned current responses based on the selective protamine transfer were obtained reproducibly even in th

279 n unclear, appears to disrupt the histone-to-protamine transition in drive-sensitive spermatids beari

280 rate corresponding defects in the histone to protamine transition.

281 ssion of select post-meiotic genes including protamines, transition protein 2, and H1fnt, all of whic

282 uld explain some adverse effects observed in protamine-treated patients.

283 ositive cells were observed in comparison to protamine treatment.

284 Protamine use remains controversial owing to concern for

285 significantly between patients who received protamine vs those who did not for the following outcome

286 replicates determination of 30.0 ng mL(-)(1) protamine was 1.26%.

287 The use of protamine was associated with a significant decrease in

288 In this work, protamine was selectively digested enzymatically to prod

289 The polyion protamine was used as a substrate to detect the activity

290 Folded bull protamine was used to condense DNA in vitro under variou

291 A DNA payload, pre-compressed by protamine, was encapsulated into the liposomes, which di

292 Sharply contrasting protamine, we show that UHRA does not interact with fibr

293 ent therapy anticoagulation with heparin and protamine were more likely to experience circuit clottin

294 Protamine, which is routinely used after cardiac surgery

295 ormed under identical conditions with a fish protamine, which lacks cysteine-rich terminal domains, d

296 calcium, chloride, alkalinity, acidity, and protamine with a range of ion-selective membranes.

297 perties, interresidue contact preferences of protamines with similar polymeric attributes suggest tha

298 biocompatibility profile similar to that of protamine, with minimal immunostimulating and systemic t

299 t the established antiangiogenic activity of protamine would rely on APJ antagonism.

300 trations of ODSH with the heparin antagonist protamine yield sharp endpoints with sensitivity to ODSH