ライフサイエンスコーパス: protease activity

1 inX1 at the site of Q50-G51 pair through its protease activity.

2 may explain how ClpT1,2 contribute to ClpPR protease activity.

3 ts chromophore incorporation is regulated by protease activity.

4 -acid modification within Pla that optimizes protease activity.

5 s, but TRAP1 is not a direct target of HTRA2 protease activity.

6 endent on T. foetus cell-associated cysteine protease activity.

7 of TAB2 and its partners, which requires the protease activity.

8 old improvement in sensitivity for detecting protease activity.

9 This cleavage relies on 3C(pro)'s cysteine protease activity.

10 d was not suppressed by inhibiting trypsin's protease activity.

11 otein often are not good indicators of total protease activity.

12 nfluences motility and virulence, as well as protease activity.

13 se of activity-based probes (ABPs) to detect protease activity.

14 s epithelial integrity on exposure to pollen protease activity.

15 e S. aureus exposure blocked the increase in protease activity.

16 neri type III effector protein with cysteine protease activity.

17 d levels of caspase 9, followed by caspase 3 protease activity.

18 ns that inhibited HCV replication and NS3/4A protease activity.

19 f microtubule regrowth also required 3C(pro) protease activity.

20 not require a significant increase in ADAM17 protease activity.

21 to inhibition of lysosomal cathepsin b-like protease activity.

22 -mediated antiviral action is independent of protease activity.

23 these positions offer least protection from protease activity.

24 and fragmented material still retaining this protease activity.

25 effects to be primarily due to HTRA1 serine protease activity.

26 ific protease gene expression and intestinal protease activity.

27 change in the capsid that induces autolytic protease activity.

28 c lipase, while they rendered an increase in protease activity.

29 FRET relay for the multiplexed detection of protease activity.

30 vel allosteric modulators of gamma-secretase protease activity.

31 ight into the spatial distribution of plaque protease activity.

32 in the transition time can be correlated to protease activity.

33 ediated Yop translocation was independent of protease activity.

34 th proteasomes actually inhibits proteasomal protease activity.

35 erate active site, rendering it incapable of protease activity.

36 uces its cleavage, which is dependent on the protease activity.

37 SFV to early endosomes or change their pH or protease activity.

38 s recruited to the MIB-IgG complex, enabling protease activity.

39 vation of plant defence independently of its protease activity.

40 None of the mutations affected NS2B-NS3 protease activity.

41 cted humans secrete wild-type levels of SpeB protease activity.

42 t major USP18 functions are unrelated to its protease activity.

43 A total of 88% of 169 strains express IgA protease activity.

44 thway independent of the encoded papain-like protease activities.

45 ssing independent of the encoded papain-like protease activities.

46 ts to be associated with certain serine endo-protease activities.

47 p a rapid and sensitive method for detecting protease activities.

48 employed to assay physiologically important protease activities.

49 rasuis (HpHtrA) exhibited both chaperone and protease activities.

50 at can be utilized for probing extracellular protease activities.

51 tforms provide limits of quantitation to ~1% protease activity (~60 pM enzyme concentration) in <1 h

52 l studies define a DNA switch triggering its protease activity, a ubiquitin switch controlling SPRTN

53 multiple peptide-based evidence on intrinsic protease activity affecting several HAdV proteins.

54 lso seen in wild-type cells lacking vacuolar protease activity after induction of a DSB.

55 activity but did not directly inhibit serine protease activity against a fluorogenic substrate specif

56 target sites of disease, sample dysregulated protease activities and emit mass-encoded reporters into

57 CPs by identifying common and organ-specific protease activities and identify previously undetected p

58 per-chilling had a significant effect on the protease activities and the ATP degradation in salmon fi

59 By altering host protease activities and the degradation patterns of prot

60 2) chimeric Sindbis virus system to evaluate protease activities and the efficacy of inhibitors direc

61 fe and efficient platforms to evaluate viral protease activities and the efficacy of protease inhibit

62 from distal unburned skin exhibited greater protease activity and a reduced capacity to inhibit bact

63 ct EV subsets that differ in composition and protease activity and are indicative of differential imm

64 strated calcium-dependent and AprI-inhibited protease activity and cytotoxicity to airway and ocular

65 rtS and slpB genes was defective in secreted protease activity and cytotoxicity to human cell lines.

66 A positive correlation between protease activity and cytotoxicity to human corneal epit

67 r islet purity was associated with increased protease activity and decreased insulin levels in cultur

68 double-mutant 3CLpro enzyme as impaired for protease activity and exhibiting reduced sensitivity to

69 CG1 and 3 of 3 CARD11 variants induced MALT1 protease activity and increased transcription from NFAT

70 rrelation exists between decreasing ZMPSTE24 protease activity and increasing disease severity, we ex

71 Canonical Wnt signaling increased protease activity and induced cartilage damage shortly a

72 ivity assays, the mutation increased calpain protease activity and made it far more active at low con

73 ted in prototypical bioassays for monitoring protease activity and nucleic acid hybridization; the la

74 regulatory signaling that modulates secreted protease activity and promotes cell wall function at hig

75 alkalinization reduces cytosolic cathepsin L protease activity and protects the podocyte cytoskeleton

76 which in turn causes decreased SpeB secreted protease activity and reduced necrotizing fasciitis capa

77 aluable a tool for the real-time analysis of protease activity and regulation.

78 The transgenic plants showed a block in Cys protease activity and SA-dependent gene expression.

79 instrumental in the detection and control of protease activity and serve as alternative methods to ge

80 The high protease activity and stability established plus the low

81 rivatives in vivo by measuring extracellular protease activity and swarming motility confirmed the in

82 p confirmed the RNAIII is required to induce protease activity and that agr cross talk modulates Ecp

83 ped here allow for rapid evaluation of viral protease activity and the identification of protease inh

84 c [Ca(2+)]i elevation, necrosis, and trypsin/protease activity and therefore has potential to effecti

85 The effects of tetrapyrrole on NS3/4A protease activity and type I IFN induction were assessed

86 egg chamber leads to localization of serine protease activity and ultimately to spatially specific a

87 uring agents can alter digestion by reducing protease activity and/or substrate solubility, and addit

88 nged with Alternaria (with or without serine protease activity), and inflammation, remodeling, and lu

89 1 with XIAP enhanced caspase-9 and caspase-3 protease activities, and CSR1-induced cell death was dra

90 uction of dead cell clearance, inhibition of protease activity, and dampening of inflammatory cell re

91 ct (CSE) and aeroallergens lacking intrinsic protease activity, and IL-6 and IL-8 production measured

92 lation of protein translation, regulation of protease activity, and response to bacterial infection.

93 SAS1B had protease activity, and SAS1B protein or antibody signifi

94 creased survival after I-R, inhibited tissue protease activity, and significantly decreased intestina

95 tructural protein 3, which negatively affect protease activity, and valine residues 785 and 787, whic

96 reduction in body temperature and decreased protease activity; and (iii) a marked redistribution of

97 Acidic lysosomal pH and increased protease activity are essential for digestion.

98 Unregulated changes in protease activity are linked to many diseases including

99 es show that the expression of BACE1 and its protease activity are tightly regulated, but the physiol

100 nes Klf2 and Klf4, as well as Rho and ADAMTS protease activity, are increased in the endothelial cell

101 Strategies that coopt protease activity as molecular triggers are increasingly

102 RadA is unlikely to possess protease activity as the putative active site serine is

103 itoleic acid ethyl ester-induced trypsin and protease activity as well as necrosis were almost abolis

104 A into picoliter-scale droplets of an HIV-1 protease activity assay to model ultraminiaturized compo

105 f a Tb(3+)-LBP-GFP energy-transfer pair in a protease activity assay.

106 nt protein (GFP) in a FRET-based homogeneous protease activity assay.

107 onstrate dose-response screening in an HIV-1 protease activity assay.

108 Sensitive, inexpensive, and rapid protease activity assays are of great merit for clinical

109 qRT-PCR and protease activity assays demonstrated that under standar

110 Protease activity associated with whole cells of Vibrio

111 purified AAV capsid preparations have robust protease activity at neutral pH but not at pH 5.5, oppos

112 mis was dependent on bacterial viability and protease activity, because killed bacteria and a proteas

113 f H3K18 as a central regulator of MMP-9 H3NT protease activity both in vitro and at H3NT cleavage sit

114 Gram-negative bacteria, possessed no general protease activity but cleaved gamma-links in both d- and

115 ered mitochondrial morphology, and decreased protease activity, but epidemiologic studies of an assoc

116 mp1 occurs in the absence of Cox20 and i-AAA protease activity, but is greatly reduced in efficiency.

117 f Rpb1 is independent of the nsP2-associated protease activity, but, instead, it proceeds through nsP

118 We determined inhibition of protease activities by monitoring the endogenous proteom

119 rity, demonstrating that control of cysteine protease activity by CF is critical for normal eosinophi

120 ism, rotation of equivalent helices controls protease activity by movement of the equivalent carbonyl

121 y is impaired due to inhibition of lysosomal protease activity by stored lipids.

122 The inhibition of protease activity by using a serine protease inhibitor l

123 proteins that respond immediately to reduced protease activity can be identified.

124 Inhibition of protease activity can block RNA replication by preventin

125 ilm formation and suggest that extracellular protease activity can influence whether Aap contributes

126 vided time- and dose-dependent factor I-like protease activity capable of cleaving C3b into inactive

127 known mechanism whereby NiV provides a novel protease activity capable of in vitro cleavage and inact

128 In vitro, inactivation of MALT1 protease activity caused reduced stimulation-induced T c

129 Alternaria-specific serine protease activity causes rapid IL-33 release, which unde

130 d after FLG knockdown included inflammation, protease activity, cell structure, and stress.

131 We showed that Der p 1, which has cysteine protease activity, cleaves the ectodomain of peptidoglyc

132 This increased protease activity coincided with increased expression of

133 letion of ATG genes or inhibition of vacuole protease activity compromises Rph1 turnover.

134 anzymes" with interdependent ion channel and protease activity conferred by a single structural domai

135 tes of AAV virus preparations also showed no protease activity contaminating the capsids.

136 In vivo, EspL cysteine protease activity contributes to persistent colonization

137 We investigated whether this surface protease activity could aid the immune evasion role thro

138 g ISGylation by specific inhibition of USP18 protease activity could constitute a promising antiviral

139 mature form, demonstrating that the cysteine protease activity critically contributes to the allergen

140 of Ca(2+) from the endoplasmic reticulum and protease activity damaged mitochondria, reduced levels o

141 The results show significant differences in protease activity depending on the assay used.

142 d syncytium formation, and endogenous serine protease activity did not contribute greatly to infectio

143 to elucidate whether allergens with a strong protease activity directly interact with modules of the

144 that growth cones use invadosomes to target protease activity during axon guidance through tissues.

145 selective pressure to decrease secreted SpeB protease activity during infection.

146 ogether, these data indicate that inhibiting protease activity during polarized tumor cell 3D migrati

147 esting a post release regulation of parasite protease activity during skin transversal.

148 -Pol protein without the spliced Pol contain protease activity equivalent to that of wild-type (WT) P

149 h sauce production, whereas those with lower protease activities for surimi processing.

150 ific SAGA-bound genes required its ubiquitin protease activity for full expression.

151 Analysis of protease activity for the preferred residues at the clea

152 tention is permanent, inhibiting any further protease activity for the remainder of its life cycle.

153 to simultaneously measure multiple specific protease activities from water-in-oil droplets that cont

154 Independent of its protease activity, FXII exerts mitogenic activity with i

155 tly cleaved MEF2D in vitro and blocking this protease activity greatly attenuated NMDAR signaled degr

156 The resulting surface plasmin protease activity has been proposed to play a role in di

157 Some allergens with relevant protease activity have the potential to directly interac

158 ved (in conjunction with increased lysosomal protease activities) higher microtubule-associated prote

159 hat the PRT6 branch of the pathway regulates protease activities in a complex manner and optimises st

160 of the present experiment was to measure the protease activities in ice-stored and super-chilled Atla

161 Specific protease activities in the sample are then inferred from

162 XH zinc-coordinated motif caused the loss of protease activities in these five S2Ps(Av), suggesting t

163 in -Cout orientation and possesses ubiquitin protease activities in vitro.

164 trates showed high protease activity, whilst protease activity in 13 culture filtrate was low.

165 Serine protease activity in Aedes aegypti saliva augmented viru

166 ls increased inflammation-regulated cysteine protease activity in atheromata and stent-induced arteri

167 r the simple detection and quantification of protease activity in buffer and human serum.

168 acellular cystatin C is linked to pathologic protease activity in cancer, arthritis, atherosclerosis,

169 loped luciferase-based biosensors to monitor protease activity in cells.

170 Elevated protease activity in cKO oviducts causes premature degra

171 ique can be used for real time monitoring of protease activity in crude preparations of virtually any

172 eviously unappreciated key function of MALT1 protease activity in immune homeostasis and underline it

173 deficiency leads to abnormally increased KLK protease activity in keratinocytes and may contribute to

174 or SPPL3, indicating a requirement for SPPL3 protease activity in NK cell biology.

175 o identify genes essential for extracellular protease activity in Serratia sp. strain SCBI and to det

176 fluorescence ratio, reliably predicted viral protease activity in single virions.

177 sue damage, further implicating dysregulated protease activity in TB-driven pathology.

178 Recent studies suggest that misregulation of protease activity in the cystic fibrosis lung may alter

179 and 1457 were identical, implicating altered protease activity in the differential Aap processing res

180 linalool did not inhibit proteasome-related protease activity in the in vitro assays.

181 r mgr3 mutations, each of which reduce i-AAA protease activity in the intermembrane space.

182 sured whether Wnt signaling led to increased protease activity in the joint.

183 (>85%) of alpha1AT in the serum and its anti-protease activity in the lung.

184 n family, that likely acts as a regulator of protease activity in the parasite.

185 soventral (DV) polarity is defined by serine protease activity in the perivitelline space (PVS) betwe

186 ine residue 117, which markedly enhances its protease activity in vitro, is critical for its ability

187 trategy allows for non-invasive detection of protease activity in vivo and ex vivo by tracking deposi

188 n keratinocytes to increase their endogenous protease activity, including specific increases in tryps

189 Whereas intracellular protease activity increased with nutrient stress, endocy

190 e effects of A1AT culture supplementation on protease activity, insulin levels, and islet function we

191 Acinar cell protease activity, insulin levels, and percent islet los

192 Thus, glucosyltransferase but not cysteine protease activity is critical for TcdB-mediated cytopath

193 Several lines of evidence suggested that protease activity is inherent in AAV capsids and is not

194 utilize peptides led us to hypothesize that protease activity is integral to the colonization proces

195 The major CAAX protease activity is mediated by Rce1 (Ras and a-factor

196 stemic dissemination, suggesting that fungal protease activity is not required for invasion during co

197 This protease activity is regulated by divalent ions and thio

198 Protease activity is required to generate viral nonstruc

199 After synthesis, protease activity is tightly controlled.

200 y in actinomycetes, exhibits both lipase and protease activities, is secreted into macrophages, and c

201 rin-dependent plasmin generation and reduced protease activity (Kcat/KM 2.57 +/- 1.02 x 10(-)(3) and

202 PMA treatment not only elevates the average protease activity level but also reduces the cellular he

203 After ischemia, plasma and tissue serine protease activity levels were increased compared to the

204 Reduction in mite protease activity limited its capacity to induce oxidati

205 lized fish species showing higher endogenous protease activities may be suitable for fish sauce produ

206 The pH-dependent protease activity may have a role in viral infection.

207 Thus, suppression of oviductal protease activity mediated by estrogen-epithelial ERalph

208 tive in reversibly inhibiting the three main protease activities of the proteasome in the micromolar

209 Notably, IRF7 cleavage strictly requires the protease activity of 3C(pro).

210 us (FMDV) 3C(pro) and that this requires the protease activity of 3C(pro).

211 The protease activity of ADAM17 is highly inducible and occu

212 Unusually, protease activity of ADAMTS13 is controlled not by natur

213 lastin, the former of which protects against protease activity of allergens and the latter with a rol

214 The protease activity of Amb a 11, as well as its capacity t

215 Targeting protease activity of Aspergillus fumigatus in conditions

216 erically and negatively regulates the serine protease activity of calnuc, inhibition being caused by

217 The crippled protease activity of Casp9-TM in the presence of the apo

218 e site, in agreement with the tryptic serine protease activity of FVIIa.

219 ient for performing functional assays of the protease activity of individual leukocytes.

220 is developed to effectively detect secretory protease activity of individual viable leukocytes.

221 nsated for this effect by reducing the basal protease activity of nsP2.

222 tations specifically disrupting the cysteine protease activity of PEDV nsp5 abrogated NEMO cleavage a

223 Mechanistically, the cysteine protease activity of PEDV nsp5 mediates proteolysis of N

224 In particular, we demonstrate that the protease activity of PepD requires the PDZ domain, in ad

225 Protease activity of Per a 10 favours Th2 responses by d

226 Protease activity of Per a 10 increased p-STAT3 levels i

227 tease inhibitor SERPINB1 counterbalanced the protease activity of PR3 in aging neutrophils, and delet

228 The protease activity of separase is strictly regulated by t

229 n of CRPC cell apoptosis independent of anti-protease activity of SLPI.

230 The pseudokinase domain might modulate the protease activity of SltB.

231 Thus, protease activity of the alphavirus capsid is a potentia

232 as well as deneddylation, facilitated by the protease activity of the CSN (COP9 signalosome), are req

233 Protease activity of the HDM extract was reduced to inve

234 y, we determined that the ubiquitin-specific protease activity of the ORF UL48 protein was functional

235 suppressant drugs, alpha-amylase protein, or protease activity of thrombin and Factor Xa.

236 passageways constantly changing due to focal protease activity of trabecular cells clearing extracell

237 Compared with the protease activity of wild-type caspase-9, that of Casp9-

238 d the effect of inhibiting allergen-specific protease activities on IL-33 levels was assessed.

239 ed pH-sensitive structural region eliminated protease activity on an external substrate but did not s

240 Control virus preparations showed no protease activity on external substrates, and filtrates

241 irus papain-like protease, altered the viral protease activity or affected viral replication or patho

242 To test the role of flexibility in protease activity, pairs of cysteines were introduced at

243 e dampened inflammation, eliminated aberrant protease activity, prevented detachment of the stratum c

244 one deacetylase (HDAC) and tumour-associated protease activities produced in malignant cancer cells.

245 We characterized protease activity profiles at single cell resolution for

246 Protease activity profiling of representatives of the PL

247 Protease activity profiling revealed a strong induction

248 We applied protease activity profiling with these new probes on Ara

249 ergic responses were dependent on subtilisin protease activity, protease-activated receptor-2, IL-33R

250 Finally, chemically blocking protease activity protected against mutant TDP-43(A315T)

251 ar stress, PI16 contributes to inhibition of protease activity; protection that can be reversed durin

252 s likely due to inhibition of ClpP-dependent protease activity rather than activation.

253 Because protease activity, rather than expression, is regulated,

254 The protease activity reached its maximum at 40 degrees C in

255 It is not understood how the protease activity required for rupture is directed with

256 tamyl peptidyl-hydrolyzing, and trypsin-like protease activities, respectively.

257 gdorferi, annotated as BB0104, having serine protease activity responsible for the primary cleavage o

258 ed a sigma factor B (DeltasigB) mutant where protease activity results in a biofilm-negative phenotyp

259 Here, we characterize bacterial CE protease activities, revealing K63-linkage-specific deub

260 casein hydrolysis assays to measure the SpeB protease activity secreted by 6,775 GAS strains recovere

261 This new method of detecting protease activity shows superior performance to conventi

262 (PrAMA), we are able to infer six different protease activity signals from individual cells in a hig

263 tural amino acids can be used to investigate protease activities/specificities for peptides containin

264 thin the hydrophobic core can modulate HIV-1 protease activity, supporting the hypothesis that drug r

265 on aeroallergens, possessed intrinsic serine protease activity that elicited the rapid release of IL-

266 ubjects, suggesting heterogeneity in Akt and protease activity that may play a role in the RA-affecte

267 small group of proteins, most with cysteine protease activity that target several key proteins invol

268 transmembrane pathway and also regulates the protease activity, thereby coupling substrate processing

269 We synthesized known activators of ClpP protease activity; these acyldepsipeptides (ADEPs) were

270 in substrate and ECM interactions, fine-tune protease activity to a particular developmental context.

271 vel compatibility factor that suppresses Cys protease activity to allow biotrophic interaction of mai

272 Here, we show that XopJ has protease activity to specifically degrade RPT6, leading

273 n the active site of Dop, suggesting a novel protease activity to target for inhibitor development.

274 s site-2 proteases (S2Ps(Av)) have authentic protease activities toward an artificial substrate pro-s

275 optode platform is used to indirectly detect protease activity (trypsin) based on proteolytic digesti

276 also established an in vitro assay for TIKI2 protease activity using FRET peptide substrates derived

277 ectrochemical biosensor for the detection of protease activity using self-assembled monolayers (SAMs)

278 The pH optimum for protease activity was acidic (5 to 6) in the gut with th

279 Secreted protease activity was characterized from 44 ocular clini

280 The capacity for multiplexed sensing of protease activity was demonstrated using these two ortho

281 Protease activity was determined using fluorogenic activ

282 However, expression of MMP-2/9 and their protease activity was elevated.

283 d by Hg(2+), Zn(2+) Co(2+) and Cu(2+), while protease activity was increased in the presence of Fe(2+

284 Protease activity was measured using fluorogenic activit

285 After activation, however, protease activity was pH-independent.

286 Tiki1 protease activity was shown to be metal ion-dependent an

287 Functionally, protease activity was significantly enhanced in Sp1-sile

288 Because higher pH activates SC protease activity, we hypothesized an enhanced release o

289 To evaluate the effect of the mutation on protease activity, we purified WT and Ubl mutant PLP2 an

290 Cathepsin K gene expression and protein and protease activity were detected in LAM-associated fibrob

291 ntrast, antimicrobial peptide production and protease activity were elevated in burn margin.

292 LAMP2 isoforms and proteasome and lysosomal proteases activities were unperturbed by LAMP-2C ectopic

293 The C-terminal part of nsP2 possesses protease activity, whereas the N-terminal part exhibits

294 1 to 95) alone of NS2B is sufficient for NS3 protease activity, whereas the role of transmembrane dom

295 concentrations and DEVDase (caspase-3 like) protease activity, which have been associated with PCD i

296 of matrix proteins by preventing the serine protease activity, which is associated with bacterial in

297 DPC processing through a unique DNA-induced protease activity, which is controlled by several sophis

298 Such a dual glycosyltransferase-protease activity, which occurs in the same active site,

299 arent WT and 5 culture filtrates showed high protease activity, whilst protease activity in 13 cultur

300 applications of biochemical methods to track protease activity, with an emphasis on the use of activi