ライフサイエンスコーパス: protein array

1 s of proteinaceous samples (e.g., tissues or protein arrays).

2 genes in renal cancer cells, we performed a protein array.

3 antified by Western blot analysis, ELISA, or protein array.

4 odology called the nucleic acid-programmable protein array.

5 nalyzed by cDNA microarray and by a cytokine protein array.

6 ared to be composed of a highly cross-linked protein array.

7 of 55 vasoactive proteins were measured with protein array.

8 0 with no-CAV were initially screened with a protein array.

9 le means of generating high-density membrane protein arrays.

10 (S-layer), which is composed of crystalline protein arrays.

11 l-transduction pathways, using reverse-phase protein arrays.

12 htforward technique for fabricating membrane protein arrays.

13 been used only sporadically for high-content protein arrays.

14 in the HGFs conditioned media using cytokine protein arrays.

15 ous flow will help expand the application of protein arrays.

16 analysis can be adapted and applied to these protein arrays.

17 tro kinase selectivity screening and phospho-protein arrays.

18 as well as in the fabrication of peptide and protein arrays.

19 ips and made important advances in preparing protein arrays.

20 and signaling molecules were measured using protein arrays.

21 this strategy to the generation of periodic protein arrays.

22 ified by both nano-LC-ESI-MS/MS and antibody protein arrays.

23 Using antibody-based protein arrays, 328 cytokines, chemokines, and receptors

24 Western blot and reverse phase protein array analyses consistently demonstrated that PI

25 Using protein array analyses, we then found that ectopic expre

26 Protein-array analyses identified host cell factors up-r

27 A protein array analysis identified IGF1 to be downstream

28 Reverse-phase protein array analysis of phospho-proteomic changes in m

29 Reverse-phase protein array analysis of PKCiota wild-type, catalytic a

30 Cytokine protein array analysis revealed that the inhibitory acti

31 Phosphoproteomic profiles from reverse-phase protein array analysis supported by mRNA profiling ident

32 Through reverse phase protein array analysis, we demonstrate that ZNF304 promo

33 tudied by Western blotting and reverse-phase protein array analysis.

34 ore, 53 angiogenic factors were examined via protein array and compared between ODN1826- and water-tr

35 ients using a validated robust reverse-phase protein array and correlated ARC levels with clinical ou

36 teractions using a nucleic acid programmable protein array and discovered that Rac1 binds to the prot

37 4, CCL5, CXCL9, and CXCL10) was confirmed by protein array and/or quantitative reverse transcription-

38 ectrometric analysis, and the development of protein arrays and biochips.

39 transcriptional profiling, and reverse-phase protein arrays and by sequencing for common breast cance

40 The combinations of protein arrays and conditioned medium arrays or serum ar

41 ession of 54 transcription factors using DNA/protein arrays and electrophoretic mobility shift assays

42 for the facile production of highly ordered protein arrays and for electron density mapping in struc

43 ungal species, and novel approaches, such as protein arrays and genomic deletion libraries, are being

44 esearch describes how to prepare immobilized protein arrays and how to assay the binding of labeled t

45 domains as a probe, we screened high-density protein arrays and identified five candidate-binding par

46 d lipid autacoid profiles were determined by protein arrays and mass spectrometry-based lipidomics.

47 els were evaluated using Q-PCR, angiogenesis protein array, and Western blotting.

48 gh-throughput analysis by similarity search, protein arrays, and affinity chromatography revealed fou

49 utism, we used immunocytochemistry, cytokine protein arrays, and enzyme-linked immunosorbent assays t

50 t of hAH was also analyzed by antibody-based protein arrays, and selected proteins were quantified.

51 combination of RNAi screening, reverse phase protein arrays, and small molecules testing, we identify

52 an be directly immobilized for biosensor and protein array applications.

53 Protein arrays are a high-throughput approach for proteo

54 DNA and protein arrays are commonly accepted as powerful explora

55 Protein arrays are described for screening of molecular

56 In addition, a reverse-phase protein array-based analysis and subsequent loss-of-func

57 ry can be effectively used to identify plant proteins arrayed by two-dimensional gel electrophoresis.

58 At least 20 copies of a protein array can be obtained from a single DNA array.

59 Such studies show that protein arrays can be used to examine in parallel the fu

60 In parallel, targeted proteomic analysis by protein array categorized the resistant samples into 3 s

61 We constructed a viral protein array comprising the complete proteomes of seven

62 An unbiased protein array confirmed this reduction in ERK and Src ac

63 In addition, His-tagged fusion protein arrays created on gold surfaces were also used t

64 We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of rep

65 exome sequencing (n = 374) and reverse-phase protein array data (n = 212) from head and neck squamous

66 ex disease datasets including microarray and protein array data demonstrate that the proposed POC-bas

67 Analysis of protein array data demonstrated lower apoptosis-associat

68 In vivo, reverse phase protein array data for tumors revealed that basal levels

69 tography-mass spectrometry and reverse phase protein array data from human MM cell lines with computa

70 Analysis of reverse-phase protein array data indicated that increased expression o

71 Reverse-phase protein array data show that PEA15 levels in invasive br

72 n breast cancer microarray and reverse-phase protein array data was performed to identify expression

73 trols) in 42 malaria (Plasmodium falciparum) protein arrays derived from 22 donors with various clini

74 Protein arrays detected several angiogenesis-related fac

75 A), which allows the 'printing' of replicate protein arrays directly from a DNA array template using

76 New technologies in protein arrays, either on chips or with self-encoded ele

77 Phospho-protein arrays elucidated the activation of protein kina

78 f Treponema pallidum subsp. pallidum using a protein array enzyme-linked immunosorbent assay.

79 Combined yeast two-hybrid and protein array experiments demonstrated that the TRD3 bin

80 Saliva was analyzed using a multiplex protein array for 30 biomarkers associated with host def

81 e report the development of a hydrogel-based protein array for quantitative and reproducible determin

82 Saliva and serum were analyzed with protein arrays for 14 pro-inflammatory and bone turnover

83 This method can be used to produce protein arrays for chip-based assays and DNA arrays for

84 ill be useful in the construction of regular protein arrays for structural analysis, in the construct

85 Protein arrays for this study were made by expressing bo

86 ties following antibody functionalization on protein arrays from undiluted human plasma and indicates

87 array) and NAPPA (nucleic acid programmable protein array) from DNA and puromycin-mediated immobilis

88 e crucial issue of design and fabrication of protein arrays have been addressed in several studies, b

89 Cell and protein arrays have demonstrated remarkable utility in t

90 d high-through-put technologies like DNA and Protein arrays have resulted in a very large amount of i

91 Protein arrays hold great promise for proteome-scale ana

92 s to yield different organized transmembrane protein arrays: (i) a bacteriorhodopsin purple membrane-

93 Screening by chemokine protein array identified a subset of melanoma cell lines

94 A protein array identified upregulation of fatty acid-bind

95 otein profiling analysis using reverse phase protein array; ii) computationally developed an Integer

96 that immobilize the probe, our reverse phase protein array immobilizes the whole repertoire of patien

97 This simple method for creating protein arrays in combination with our analyses of sever

98 dent proteins were measured by Reverse Phase Protein Arrays in hippocampus and cortex, and 64 in cere

99 ce-based protein detection limits the use of protein arrays in research and clinical diagnosis.

100 important autoantibody targets identified on protein arrays included Myc-associated zinc finger prote

101 Using a protein array, it was determined that LMP1 selectively a

102 titatively assess the quality of gel element protein arrays manufactured with whole-cell lysate in vi

103 ose a metadata format known as reverse phase protein array markup language (RPPAML).

104 s such as laser-capture mass spectrometry or protein arrays may be helpful as well.

105 l approach to designing two-dimensional (2D) protein arrays mediated by noncovalent protein-protein i

106 Using the yeast two-hybrid system and a protein array membrane, we identified two novel protein

107 In situ or on-chip protein array methods use cell free expression systems t

108 rofluidic-based comprehensive human membrane protein array (MPA).

109 rray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of p

110 s fabricated using nucleic acid programmable protein array (NAPPA) technology, which enables the high

111 In one approach, we constructed a protein array of about 6,000 yeast transformants, with e

112 ur method has the advantage over traditional protein arrays of being able to detect autoimmunity to e

113 Protein arrays of liver homogenates from patients with a

114 escence and analyzed angiogenic molecules by protein arrays of lysates and cell culture supernatants.

115 Nevertheless, the proteins arrayed on this lattice necessarily have an int

116 icle, we describe the fabrication in situ of protein arrays optimized for MALDI characterization.

117 s vasoactive protein levels were measured by protein array or enzyme-linked immunosorbent assay.

118 ces such as electronic biosensors, thin-film protein arrays, or biofuel cells.

119 nstrate the potential to use the multiplexed protein array platform for respiratory disease diagnosis

120 oma, we used a high-throughput reverse-phase protein array platform to identify signaling alterations

121 For protein arrays, potential advantages of this format are

122 Employing a protein array produced in vitro from 1,292 DNA fragments

123 The protein array provided a sensitive platform for global s

124 ys, but also for other technologies, such as protein arrays, quantitative mass spectrometry and next-

125 as measured by gene expression and cytokine protein array, respectively.

126 A reverse-phase protein array revealed that HuR-mediated regulation of P

127 Reverse-phase protein array revealed that the Ras-dependent downstream

128 Our screening, using a reverse-phase protein array, revealed distinct mechanisms by which PAK

129 nic mice by RNA sequencing and reverse phase protein array reveals modulation of oncogenic networks o

130 the Braf-mutant cell lines by reverse phase protein array (RPPA) analysis showed no significant asso

131 ptional response by exploiting reverse phase protein array (RPPA) and mRNA expression data in The Can

132 g with a functional proteomics reverse-phase protein array (RPPA) platform assessing 42 phospho and t

133 High-throughput reverse-phase protein array (RPPA) technology allows for the parallel

134 In this study, we used reverse phase-protein array (RPPA) technology to measure changes in mu

135 types using the antibody based reverse phase protein array (RPPA) technology.

136 after radiation, we utilized a reverse phase protein array (RPPA) to identify significant changes in

137 Reverse phase protein array (RPPA) was performed on 205 proteins isola

138 129 ALL patient samples using reverse phase protein array (RPPA) with ErbB2 and phospho-ErbB2 antibo

139 report a novel functionalized reverse phase protein array (RPPA), termed polymer-based reverse phase

140 Reverse Phase Protein Arrays (RPPA) are convenient assay platforms to

141 Reverse phase protein arrays (RPPA) measure the relative expression le

142 Reverse-phase protein arrays (RPPA) represent a powerful functional pr

143 Here, we describe the use of Reverse Phase Protein Arrays (RPPAs or RPLAs) to profile signaling pro

144 Reverse phase protein arrays (RPPAs) are a powerful high-throughput to

145 A reverse-phase protein array screen identified SRC3/AIB1 as one oncopro

146 In a protein array screen, we identify a cluster of mitotic e

147 Using high-throughput protein array screening and bioinformatic analysis, we i

148 We also used Reverse Phase Protein Array screening to identify differential protein

149 Using protein array screening, we showed PTTG1 interacting wit

150 ly specific antibody-antigen interactions by protein array screening.

151 gingivalis and analyzed by a phosphorylation protein array show a reduction of Akt only in BMMPhi fro

152 converting only part of the DNA array into a protein array, simultaneous detections of DNA and protei

153 With the subsequent design of SPR protein arrays, SPR-MS is expected to enter into the fie

154 Unbiased reverse phase protein array studies and subsequent validation experime

155 Reverse phase protein array suggested that high expression of the esse

156 g of multiple cytokine expression based on a protein array system.

157 should allow us to develop many high-density protein array systems to detect a variety of proteins.

158 up-regulation of PKCbeta protein in WM using protein array techniques and immunohistochemistry.

159 When coupled with new protein array technologies, high-throughput SPR methods

160 strategies should increase the usefulness of protein array technologies.

161 e, a robust strategy comparable with gene or protein array technology for comprehensively analyzing t

162 We have developed a multianalyte fluid-phase protein array technology termed high-throughput immunoph

163 ies (autoAbs) in PV and (ii) use multiplexed protein array technology to define the scope and specifi

164 We used protein array technology to identify the MAP3K2 kinase a

165 These methods address three issues in protein array technology: (i) efficient protein expressi

166 We describe a format for production of protein arrays termed 'protein in situ array' (PISA).

167 e responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptid

168 re spanned by asymmetric membrane-associated protein arrays that had approximately 6-nm lateral perio

169 In contrast to previous protein arrays that immobilize the probe, our reverse ph

170 Protein arrays that measure multiple protein cancer biom

171 Thus, instead of a holey protein array, the experimental model presented here app

172 biochemical activities, the construction of protein arrays, the identification of interactions, and

173 We first identified, by reverse-phase protein arrays, the lymphocyte cell-specific protein-tyr

174 Reverse phase protein array, tissue microarray, and quantitative flow

175 We generated an Epstein-Barr virus (EBV) protein array to evaluate the targets of the EBV protein

176 We used reverse-phase protein array to measure the transient changes in the ph

177 ctroscopy-based sequencing and reverse-phase protein arrays to 547 human breast cancers and 41 cell l

178 Here we use reverse-phase protein arrays to analyse 3,467 patient samples from 11

179 tributed or pre-arrayed DNA or RNA, enabling protein arrays to be created on demand.

180 are now being developed for construction of protein arrays to detect the presence of antibodies agai

181 We also use reverse-phase protein arrays to generate comprehensive, quantitative S

182 loratory Stage I we utilized high-throughput protein arrays to identify 35 high-interest proteins sho

183 Here, we used high-density protein arrays to identify non-HLA antibodies in CAI and

184 We describe the use of protein arrays to identify proteins that bind to DNA seq

185 Here, we used high-density protein arrays to identify target antigens for AECAs iso

186 ts were also used with the His-tagged fusion protein arrays to study multiple antibody-antigen bindin

187 We have used protein arrays to test 492 pairings of a nearly complete

188 Here I report the development of a universal protein array (UPA) system that provides a sensitive, qu

189 Existing approaches to quantify protein arrays use parametric response curves fit to dil

190 We report the fabrication of a patterned protein array using three orthogonal methods of immobili

191 characterization of histidine-tagged fusion protein arrays using nitrilotriacetic acid (NTA) capture

192 proach to infer spatial relationships within protein arrays using stimulated emission depletion nanos

193 It was designed for the production of protein arrays using the ion soft-landing technique to c

194 In addition, the GST-Eps15 protein array was capable of detecting inhibition of EGF

195 A protein array was performed on NOD2-activated enterocyte

196 f the proteins screened in the reverse-phase protein array, we found that insulin receptor substrate

197 y, with the use of microfluidic networks and protein arraying, we demonstrate the potential of this c

198 Here, using oligonucleotide and protein arrays, we analyze the infection of primary pola

199 Using reverse-phase protein arrays, we generated ovarian carcinoma protein e

200 Using high density protein arrays, we have previously identified in vitro s

201 Using reverse-phase protein arrays, we measured expression levels of approxi

202 By screening sera with protein arrays, we showed that our treatment can induce

203 enitor cells using a validated reverse-phase protein array; we correlated its levels with clinical ou

204 All binding interactions occurred at 5x4 protein array were real-time monitored simultaneously.

205 Periodic protein arrays were achieved by templated self-assembly

206 Oriented protein arrays were fabricated using a two-step process:

207 In conclusion, protein arrays were used to identify a novel SPTLC1-Par3

208 more than 30 identical copies of a membrane protein array while requiring only femtomoles of protein

209 teraction motif, and by screening PDZ domain protein arrays with an SPTLC1 C-terminal peptide, we fou

210 The CFM can be used to deposit protein arrays with good spot morphology, spot-to-spot u

211 The existence of protein arrays, with a large selection of different kind

212 trates with equipment commonly used to print protein arrays, without complex surface chemistry protoc