ライフサイエンスコーパス: protein engineering

1 otein-protein interactions is a key issue of protein engineering.

2 control of structure and function enabled by protein engineering.

3 s a model for the therapeutic application of protein engineering.

4 lent bond formation for protein research and protein engineering.

5 ilable for both organometallic synthesis and protein engineering.

6 s (UAAs) into proteins is a powerful tool in protein engineering.

7 ide future experiments with SMPLs applied to protein engineering.

8 atable multistate protein is a challenge for protein engineering.

9 templates for combinatorial and data-driven protein engineering.

10 nse of proteins to hydrostatic pressure, and protein engineering.

11 ne dehalogenases, and a mutant stabilized by protein engineering.

12 interaction and function is a challenge for protein engineering.

13 azine-containing polymer using polymer-based protein engineering.

14 gh-pressure structural methods as a tool for protein engineering.

15 found many applications in biotechnology and protein engineering.

16 ected evolution has transformed the field of protein engineering.

17 tion, evolutionary developmental biology and protein engineering.

18 ecular evolution and a valuable strategy for protein engineering.

19 tein libraries are essential to the field of protein engineering.

20 lling the outcome of assembly by scaffolding protein engineering.

21 tion of sesterterpenes through metabolic and protein engineering.

22 acid sequence or to modify the structure by protein engineering.

23 unction annotation, mutagenesis analysis and protein engineering.

24 s; these therapeutic proteins are made using protein engineering.

25 s are capable of further improvement through protein engineering.

26 al of ancestral reconstruction as a tool for protein engineering.

27 computational/directed evolution approach to protein engineering.

28 sms and cell types attests to the success of protein engineering.

29 a useful approach to enforce dimerization in protein engineering.

30 lications including rational drug design and protein engineering.

31 to fundamental tools that underlie rational protein engineering.

32 ation is a valuable tool for drug design and protein engineering.

33 ins is well established as a useful tool for protein engineering.

34 ETD combined with functional assays to guide protein engineering.

35 is a key objective of synthetic biology and protein engineering.

36 other positions for increasing stability in protein engineering.

37 otein design (SCPR) is an important topic in protein engineering.

38 at this dependence can be exploited to guide protein engineering.

39 unction of the proteome, as well as to guide protein engineering, accurate in silicomethodologies are

40 s demonstrate the power of the technique for protein engineering, affinity reagent discovery and stru

41 We report a strategy for protein engineering allowing an organized mixing and mat

42 We propose that through structure-based protein engineering, an improved D8 tetramer could be us

43 ue innovative mutants, with implications for protein engineering and adaptive evolution.

44 Here we use force spectroscopy, protein engineering and bacterial growth assays to inves

45 Cross-beta's recalcitrance to protein engineering and conspicuous absence among the kn

46 rected Precambrian proteins as scaffolds for protein engineering and demonstrate that a new active si

47 , these data validate AgRP as a scaffold for protein engineering and demonstrate that modification of

48 These results demonstrate how modern protein engineering and design tools can facilitate the

49 structure-dynamics-function relationship in protein engineering and design.

50 re prediction, protein model assessment, and protein engineering and design.

51 and their importance for future advances in protein engineering and design.

52 acks can be minimized or eliminated by using protein engineering and directed evolution, resulting in

53 ated cell sorting (FACS) is a common task in protein engineering and directed evolution.

54 of functional relationships among proteins, protein engineering and drug design.

55 evolves will provide guiding principles for protein engineering and function prediction.

56 ecular evolution with broad implications for protein engineering and function prediction.

57 improving hybrid recombinase specificity by protein engineering and illustrate the potential of thes

58 nd represents a novel alternative method for protein engineering and in vitro directed protein evolut

59 Through protein engineering and in vivo functional tests, we rep

60 ly occurring biocatalysts is a challenge for protein engineering and is a critical test of our unders

61 Here, we describe a combination of protein engineering and kinetic, spectroscopic, and biop

62 The results provide a guide for protein engineering and large-scale mutagenesis enabled

63 Advances in protein engineering and materials science have contribut

64 olecule force spectroscopy AFM combined with protein engineering and MD simulations to study the indi

65 arious improvements in technology, including protein engineering and microfocus X-ray diffraction.

66 We combine structure-based protein engineering and molecular genetics to restrict t

67 Protein engineering and mutagenesis studies have suggest

68 Merging protein engineering and nanotechnology offers exciting p

69 determining structures of cleavable forms by protein engineering and native-state proteolysis.

70 bility previously observed in computational, protein engineering and NMR dynamics studies, demonstrat

71 omized libraries are increasingly popular in protein engineering and other biomedical research fields

72 we use T-jump relaxation in conjunction with protein engineering and report mutational Phi-values (Ph

73 ein circularization can significantly impact protein engineering and research in protein folding.

74 Using membrane protein engineering and single-channel electrical record

75 zole inhibitor by applying a high-throughput protein engineering and surface-site mutagenesis approac

76 At the same time, protein engineering and synthetic biology have expanded

77 ilored stabilities would facilitate rational protein engineering and synthetic biology.

78 we chart the development of omega-TAms using protein engineering and their contribution to elegant on

79 tein termini reorganization, we have applied protein engineering and x-ray crystallography to cp283,

80 Using ancestral gene reconstruction, protein engineering and X-ray crystallography, we demons

81 peptides, review advances in enzymology and protein engineering, and discuss the regulatory networks

82 crosslinking has important implications for protein engineering, and its sensitivity to chemical inh

83 namic and kinetic stability, immobilization, protein engineering, and medium engineering of biocataly

84 ing, steered molecular dynamics simulations, protein engineering, and single-molecule force spectrosc

85 ngle molecule atomic force microscopy (AFM), protein engineering, and steered molecular dynamics (SMD

86 in computational biology, molecular biology, protein engineering, and systems biology to design, synt

87 al scans may aid structure-function studies, protein engineering, and the interpretation of variants

88 ental strategy centered on NMR spectroscopy, protein engineering, and X-ray crystallography.

89 //zf.princeton.edu and can be used to aid in protein engineering applications and in genome-wide sear

90 ts the potential of the SRS-ACC scaffold for protein engineering applications and provides insight in

91 increasingly important not only to optimize protein engineering applications in areas as diverse as

92 volution of gas pathways in proteins and for protein engineering applications involving modifications

93 gical parts, the use of synthetic biology in protein engineering applications, and the engineering of

94 g neural activity-dependent sensors, and our protein engineering approach can be generalized to creat

95 Additionally, a protein engineering approach is presented whereby split

96 address this, we describe a mechanism-guided protein engineering approach that imbues ultrafast DnaE

97 A protein engineering approach to delineating which distin

98 Here we develop a protein engineering approach to directly tune in vivo ca

99 We here describe a rationale-based protein engineering approach to generate a potent long-a

100 ne superresolution imaging techniques with a protein engineering approach to investigate how such nan

101 Chowdhury, et al. use a protein engineering approach to render a temperature-ins

102 Moreover, this study provides an alternative protein engineering approach to the design of a carbohyd

103 Our study, through a structure-based protein engineering approach, offers a novel strategy fo

104 Using a rational protein engineering approach, we interconverted the enzy

105 idoids"), coupled with a reversible chemical protein engineering approach.

106 logy but altered regional stability, using a protein engineering approach.

107 e system is a biofuel cell cathode, but this protein-engineering approach to advanced functional hydr

108 SAM analogue, in combination with a rational protein-engineering approach, thus shows potential to la

109 Using a protein-engineering approach, we generated high-affinity

110 ase activity and pave the way to explore new protein engineering approaches aimed at designing redox-

111 then present experimental and computational protein engineering approaches for control of protein fu

112 nts in the use of rational and combinatorial protein engineering approaches to developing ligands and

113 ession system for hCPS allowed us to utilize protein engineering approaches to elucidate the distinct

114 odel of SbCCoAOMT can serve as the basis for protein engineering approaches to enhance the nutritiona

115 Directed evolution and protein engineering approaches used to generate novel or

116 d describe several chemical modification and protein engineering approaches used to improve the life

117 ousand-fold are now routine for a variety of protein engineering approaches, and the structural and e

118 mbining computational structure modeling and protein engineering approaches, we uncovered a conformat

119 rapeutic proteins and describe some emerging protein-engineering approaches that might prevent the de

120 Guided by this structure, we use protein-engineering approaches to show that intein-succi

121 Using two protein-engineering approaches, we achieve independent c

122 The principles of natural protein engineering are obscured by overlapping function

123 However, protein engineering as a strategy has not yet been used

124 sed recorders, providing guidance for future protein engineering attempts.

125 The methodology developed permits protein engineering based on dynamical network optimizat

126 that obtaining 3D(pol) fidelity variants by protein engineering based on polymerase structure and fu

127 gp120 is a substrate for protein engineering both for human immunodeficiency viru

128 uch as biocatalysis, live-cell vaccines, and protein engineering but also for gaining mechanistic ins

129 rrangement provides unique opportunities for protein engineering by functional derivatization of thes

130 dRP active site closure and demonstrate that protein engineering can be used to alter viral polymeras

131 catalysis is paramount to its function, and protein engineering can be used to reprogram the cycliza

132 Protein engineering can provide APC mutants that permit

133 rystals was enabled through a combination of protein engineering, chaperone coexpression, modificatio

134 sophisticated function and introduces a new protein-engineering concept that allows for the generati

135 rsity of peptide domains utilized in modular protein engineering continues to expand, a tremendous an

136 Protein engineering could exploit this potential further

137 Improving DGAT activity using protein engineering could lead to improvements in seed o

138 Protein engineering demonstrated that only the ameloblas

139 ements are essential in such applications as protein engineering, drug development, protein design an

140 y concept presents an attractive strategy to protein engineering, e.g., to create new scaffolds for e

141 This assay has the potential to guide protein engineering efforts and identify stabilizing con

142 Supporting this view, protein engineering efforts to incorporate D-amino acids

143 bility could be a viable target for membrane protein engineering efforts.

144 me features, thus streamlining and informing protein engineering efforts.

145 This extensive protein engineering encompassed the entire cork domain a

146 Protein engineering experiments and ancestral-state reco

147 l have broad applications in high-throughput protein engineering experiments and functional genomics.

148 We performed protein engineering experiments that revealed pervasive

149 e SFLD in correcting misannotations, guiding protein engineering experiments, and elucidating the fun

150 ther is a common event in both evolution and protein engineering experiments.

151 view will discuss the unique challenges that protein engineering faces in the process of converting l

152 ate engineering as an orthogonal approach to protein engineering for modulation of regioselective C-H

153 at viral capsids can be greatly stiffened by protein engineering for nanotechnological applications.

154 Due to this deficiency, protein engineering frequently relies on the use of comp

155 re useful tools for antigen optimization and protein engineering generally.

156 is study, we present a successful example of protein engineering, guided by structural insight on the

157 ccess seen in other industrial applications, protein engineering has achieved only modest results in

158 d manipulation of cytokine signaling through protein engineering has become an increasingly feasible

159 Protein engineering has been used broadly to overcome we

160 Protein engineering has been used to improve the perform

161 Protein engineering has enabled the optimization of exis

162 Protein engineering has generated versatile methods and

163 ion of this bioreducible lipid platform with protein engineering has the potential to advance the the

164 Recent advances in protein engineering have led to the emergence of antibod

165 Recent efforts in protein engineering have significantly increased the per

166 irected evolution is a powerful strategy for protein engineering; however, evolution of pharmaceutica

167 ysis is an important step of drug design and protein engineering in order to predict the binding affi

168 pose the somatic hypermutation machinery for protein engineering in situ.

169 The findings presented have implications in protein engineering, in design of accelerated stability

170 These results suggest new directions in protein engineering, in that modifying glycosylation pat

171 Protein engineering is a powerful tool for designing or

172 Protein engineering is an important tool for the design

173 Protein engineering is becoming increasingly important f

174 Although the practice of protein engineering is industrially fruitful in creating

175 the interaction between Fc and FcRn through protein engineering is one method for improving the phar

176 re typically based on endogenous enzymes, so protein engineering is required to ensure that the small

177 An important task of protein engineering is to identify alternative sequences

178 uality native LHEs to serve as scaffolds for protein engineering-many are unsatisfactory for gene tar

179 ioremediation by utilizing rhizoremediation, protein engineering, metabolic engineering, whole-transc

180 A highly combinatorial structure-based protein engineering method for obtaining enantioselectiv

181 We present a baculovirus-based protein engineering method that enables site-specific in

182 CD80 and CD86, we employed a high-throughput protein engineering method to map the ligand binding sur

183 the current capabilities of the widely used protein engineering method, expressed protein ligation.

184 Alternate frame folding (AFF) is a protein engineering methodology the purpose of which is

185 state for R15 folding is investigated using protein engineering methods (Phi-value analysis) and com

186 cterial signaling and limitations of current protein engineering methods combine to make reprogrammin

187 they are designed and optimized using facile protein engineering methods, and self-assembled in cells

188 g biophysical techniques in conjunction with protein engineering methods, including segmental isotopi

189 Protein-engineering methods (Phi-values) were used to in

190 our understanding of allostery, and advance protein-engineering methods for manipulating the O 2 bin

191 een harnessed for the development of several protein-engineering methods.

192 This system has general utility in protein engineering, molecular biology, and disease rese

193 Here we summarize the innovations in both protein engineering/molecular biology and crystallograph

194 This review covers the topic of protein engineering of cellulases, mostly after 2009.

195 Protein engineering of cytochrome P450 monooxygenases (P

196 tabolism our study provides a foundation for protein engineering of enone oxidoreductases and their a

197 om this work represent a platform for future protein engineering of FhuA that will be employed for sp

198 We used protein engineering of mouse APC and genetically altered

199 tion, expression of regulatory elements, and protein engineering of P450s.

200 tect protein-ligand interactions without any protein engineering or chemical modification.

201 In addition to the use of protein engineering, other aspects of biocatalysis engin

202 Unlike previous approaches to tandem repeat protein engineering, our design procedure does not rely

203 ionally manipulated to significantly improve protein engineering outcomes.

204 Polymer-based protein engineering (PBPE) offers a unique method to tai

205 Advances in protein engineering permit the design and/or directed ev

206 y path to nascent enzymatic activity; from a protein engineering perspective, future efforts in de no

207 Using a protein engineering Phi-value analysis to probe the mech

208 lean slate on which to define and test these protein engineering principles, while recreating and ext

209 cal experiment design, mutagenesis analysis, protein engineering, protein design, biological pathway

210 ly CREATE to site saturation mutagenesis for protein engineering, reconstruction of adaptive laborato

211 s are of suitable quality for computer-based protein engineering: redesigned models and redesigned X-

212 Extensive directed evolution and protein engineering resulted in seventh-generation ABEs

213 cological activity, validating cyclotides as protein engineering scaffolds.

214 Extensive protein engineering showed thermodynamic linkage between

215 cule probe reaction circumvents the need for protein engineering, since these enzyme activities are n

216 Here, we combine protein engineering, single-molecule force spectroscopy,

217 Almost all protein engineering so far has involved the modification

218 alised crystallography techniques as well as protein engineering solutions such as fusions and confor

219 recognition, carbon turnover in nature, and protein engineering strategies for biomass utilization.

220 Protein engineering strategies seek to develop a hemoglo

221 The optical and protein engineering strategies that form the basis of th

222 crystal structure makes it possible to test protein engineering strategies to develop a monovalent b

223 ues, mutant receptors generated via rational protein engineering strategies were examined for improve

224 Protein engineering strategies, guided by biological pri

225 sulted from the development of GPCR-specific protein engineering strategies.

226 regulatory properties of the system through protein engineering strategies.

227 ove catalytic performance, we have applied a protein engineering strategy called circular permutation

228 Here we describe a protein engineering strategy coupled with small-molecule

229 To tackle this problem, we devised a protein engineering strategy for rational design of inhi

230 ar hormone receptors, we anticipate that our protein engineering strategy will be applicable to the c

231 itecture may represent a new kind of modular protein-engineering strategy for designing light-activat

232 We have established a new protein-engineering strategy termed "directed domain-int

233 el involved in pain perception, we present a protein-engineering strategy that has allowed us to dete

234 Building on previous protein engineering studies and guided by a structural m

235 Protein engineering studies demonstrated that it is poss

236 Recently, a number of successful protein engineering studies have been reported that aime

237 Protein engineering studies often suggest the emergence

238 We describe protein engineering studies on 5-carboxymethylproline sy

239 he PcISPS structure promises to guide future protein engineering studies, potentially leading to hydr

240 We describe an in vivo protein engineering system and its use in characterizing

241 Here we use a combination of protein engineering techniques and single-molecule force

242 Protein engineering techniques have emerged as powerful

243 ade C virus, CZA97.012, by using an array of protein engineering techniques to improve a prototypic c

244 ic force microscopy, cyclic voltammetry, and protein engineering techniques to investigate directly h

245 In this study, we have employed protein engineering techniques to investigate the C-term

246 mbine single molecule force spectroscopy and protein engineering techniques to investigate the mechan

247 Using protein engineering techniques, we created a full-length

248 logy are highlighted and contrasted to other protein engineering techniques.

249 eral of these problems through computational protein engineering techniques.

250 Using protein-engineering techniques, we generated a monomeric

251 we address the promise of applying emerging protein engineering technologies to cardiovascular medic

252 s been exploited to develop several powerful protein engineering technologies.

253 ediates was subsequently exploited for a new protein engineering technology called MAD-TRAP (membrane

254 evelopment; however, these typically require protein engineering that alters Env structure.

255 ive Paths (REAP), for directed evolution and protein engineering that exploits phylogenetic and seque

256 Using selective protein engineering that involves disulfide bond formati

257 plied in biosynthesizing new polyketides via protein engineering that rationally controls polyketide

258 tionally allowed in silico-designed targeted protein engineering that unlocked the path to alternate

259 By exploiting substrate promiscuity and protein engineering, the scope of reactions catalysed by

260 n of selenocysteine is of great interest for protein engineering, the sequence constraints imposed by

261 In the case of protein engineering, there are three main approaches to

262 Here, we report an approach that exploits protein engineering to "humanise" thermophilic archeal s

263 inefficient at first trials, can be tuned by protein engineering to allow atomic-resolution NMR studi

264 ccessfully altered through several rounds of protein engineering to an enantioselective amine dehydro

265 y natural enzymes have been modified through protein engineering to better suit practical application

266 ing from combinatorial library screening and protein engineering to bioremediation and biofuels produ

267 electron-electron resonance spectroscopy and protein engineering to confirm predictions of our comput

268 We have used structure-informed protein engineering to create a recombinant, GFP-tagged

269 s confirm the advantages of structure-guided protein engineering to design improved low-calorie sweet

270 nstrumentation, and prospects for the use of protein engineering to develop the sensitivity and selec

271 yltransferase Dnmt3a as a paradigm, we apply protein engineering to dissect the molecular interaction

272 sed importance of results from computational protein engineering to drive ideas in the field, as expe

273 , and this catalytic efficiency has inspired protein engineering to enable its exploitation for biote

274 We combined chemistry and protein engineering to enable the systematic creation of

275 rcing CAs from thermophilic organisms, using protein engineering to evolve thermo-tolerant enzymes, i

276 ngle-channel electrical recording along with protein engineering to examine a protein-protein pore in

277 The crevice is amenable to protein engineering to further enhance both specificity

278 We used protein engineering to generate functional Hsp90 subunit

279 ty acid hydroxylation in OleTJE could enable protein engineering to improve catalysis or to introduce

280 Here, we use structure-guided protein engineering to improve the specificity of Strept

281 ion can be readily manipulated by biology or protein engineering to significantly affect association

282 single-molecule force-clamp spectroscopy and protein engineering to study the effect of force on the

283 framework presented here may prove useful in protein engineering to tune metal selectivity.

284 ated that "Velcro" engineering is a powerful protein-engineering tool with potential applications to

285 lity, and contribute additional tools to the protein engineering toolkit.

286 nt, fueled by both an enhanced repertoire of protein engineering tools and an increasing list of solv

287 arness molecular, genetic, microbiology, and protein engineering tools and rely on identification of

288 ristics of these channels can be modified by protein engineering tools and the channels can be functi

289 licing reaction and have emerged as valuable protein engineering tools in numerous and diverse biotec

290 o demonstrate the utility of this module for protein engineering, two rounds of directed evolution we

291 sequences could be addressed for cleavage by protein engineering, ushering in the breakthrough in gen

292 In the present study, protein engineering was performed to swap or replace key

293 ore vestibule modeling, and structure-guided protein engineering, we designed and characterized a cla

294 Through structure-guided protein engineering, we generated several BAR variants t

295 Here, using hydrogen-exchange-directed protein engineering, we populated the folding intermedia

296 ined from nArmRP through cycles of extensive protein engineering, which rendered them more uniform.

297 teomics, biology, biomarkers, chemistry, and protein engineering will coalesce to accelerate the deve

298 This strategy in protein engineering will open avenues to explore the bio

299 re, using lipid-mediated crystallization and protein engineering with a novel fusion chimaera, we sol

300 The combination of the advances in protein engineering with electrical and/or optical signa