ライフサイエンスコーパス: protein splicing

1 a different mechanism that does not require protein splicing.

2 kinetic analysis of the individual steps of protein splicing.

3 rminal glutamine, is capable of facilitating protein splicing.

4 ng a nine-residue FGF-5 peptide generated by protein splicing.

5 s and as tools for studying the mechanism of protein splicing.

6 e CcdB toxin unless the intein is excised by protein splicing.

7 sis of structural and mechanistic aspects of protein splicing.

8 ndicating their potential functional role in protein splicing.

9 s facilitated the study of the modulation of protein splicing.

10 published and unpublished information about protein splicing.

11 nserved residues in block F are required for protein splicing.

12 initely a close evolutionary relationship to protein splicing.

13 nctional roles of some conserved residues in protein splicing.

14 autocatalytically excise themselves through protein splicing.

15 tein structure and the unusual enzymology of protein splicing.

16 as able to reactivate the intein and trigger protein splicing.

17 and two His residues that are implicated in protein splicing.

18 rearrangement constitutes the first step in protein splicing.

19 o interrogate the mechanism of non-canonical protein splicing.

20 trapped in the branched intermediate step in protein splicing.

21 inally, we describe instances of conditional protein splicing.

22 o an active conformation that promotes rapid protein splicing.

23 highly efficient Cre-reconstitution through protein splicing.

24 important role in coordinating the steps of protein splicing.

25 e in transesterification, the second step of protein splicing.

26 ribute to the higher optimal temperature for protein splicing.

27 ination mechanism for the first two steps of protein splicing.

28 romote the N-S acyl shift, the first step of protein splicing.

29 us of the intein, which are active sites for protein splicing.

30 as a dual role in the acid-base catalysis of protein splicing.

31 r intermediate formed during intein-mediated protein splicing.

32 n that prevent the second and third steps of protein splicing.

33 ius or higher, the intein mediates efficient protein splicing.

34 e moderately improves the rate and extent of protein splicing.

35 ures of domains with single endonuclease and protein splicing active sites.

36 in the protein-splicing process and that the protein-splicing active center is confined to the N- and

37 We speculate that this protein splicing activity arose to post-translationally

38 Their protein splicing activity is essential for the host prot

39 th hyper- and hypophosphorylation inhibit SR protein splicing activity, repressing constitutive splic

40 137 residues may be the lower limit for full protein-splicing activity.

41 erminal splicing junctions, blocking in vivo protein splicing, allowed the miniprecursor to be purifi

42 Some of the reactions characteristic of protein splicing also occur in other forms of protein au

43 uld be useful in studies of the mechanism of protein splicing and allow structural studies of unmodif

44 ents (inteins) function both as catalysts of protein splicing and as homing endonucleases.

45 in, the renatured fusion protein can undergo protein splicing and chromophore formation.

46 First, we demonstrated that both protein splicing and cleavage at the N-terminal splice j

47 1 substitutions attenuated the initiation of protein splicing and enabled us to demonstrate in vitro

48 The PI-SceI protein splicing and endonucleolytic active sites are se

49 eI has a bipartite domain structure, and the protein splicing and endonucleolytic reactions are catal

50 utamine in the C-terminal position, prevents protein splicing and facilitates cleavage at the intein

51 ell as strategies for modulating the rate of protein splicing and for converting the splicing reactio

52 ) of Saccharomyces cerevisiae catalyzes both protein splicing and site-specific DNA cleavage.

53 hat propagates its mobile gene by catalyzing protein splicing and site-specific DNA double-strand cle

54 e junction residues are directly involved in protein splicing and the central dodecapeptide motifs ar

55 nput, enabling enhanced genetic control over protein splicing and the potential creation of splicing-

56 Hedgehog/INTein (HINT) domains catalyzing protein splicing and their nested HEN domains are though

57 this alignment (Cfa) demonstrates both rapid protein splicing and unprecedented thermal and chaotropi

58 screening system detects even low levels of protein splicing and we have used it to show that protei

59 acid Mtu recA intein consists of independent protein-splicing and endonuclease domains.

60 The disulfide bond inhibits protein splicing, and splicing can be induced by reducin

61 an undecapeptide spacer) was able to promote protein splicing as efficiently as the wild-type intein,

62 protein splicing by zinc ion, a fluorometric protein splicing assay was developed in which the denatu

63 es an in vitro fluorometric assay system for protein splicing based on the RecA intein of Mycobacteri

64 hat is not only catalytically sufficient for protein splicing but also structurally independent from

65 at the -1 position had no effect on overall protein splicing but could lead to significant accumulat

66 None affects protein splicing, but one, a R417Q substitution, account

67 artate (D422) coordinates different steps in protein splicing, but the precise mechanism is unclear.

68 be an anomaly found in only a few organisms, protein splicing by inteins has since been observed in m

69 ein splicing inhibitors, which may attenuate protein splicing by less than an order of magnitude, we

70 fragments, which could be induced to undergo protein splicing by reduction of the disulfide bond.

71 ng advantage of the reversible inhibition of protein splicing by zinc ion, a fluorometric protein spl

72 Protein splicing can be modulated by mutation and conver

73 These results indicate that Prp8 protein splicing can be modulated, with potential functi

74 We demonstrate for the first time that protein splicing can proceed efficiently after the remov

75 When diluted into appropriate buffers, protein splicing could be initiated by the addition of a

76 uce a NIR optogenetic module for conditional protein splicing (CPS) based on the gp41-1 intein.

77 n this report, we show that this conditional protein splicing (CPS) system can be used in mammalian c

78 bility to control PTS, so-called conditional protein splicing (CPS), has led to the development of to

79 vage site region of the substrate, while the protein splicing domain (domain I) interacts with a dist

80 The majority of inteins are comprised of a protein splicing domain and a homing endonuclease domain

81 the codon for Arg94, which is located in the protein splicing domain and makes essential contacts to

82 Conversely, residues 91, 97, and 170 in the protein splicing domain are in close proximity to a dist

83 experimental evidence demonstrates that the protein splicing domain as well as the endonuclease doma

84 t of two functionally independent domains, a protein-splicing domain and an endonuclease domain.

85 rrored by the functional independence of the protein-splicing domain, the entire endonuclease compone

86 These naturally occurring protein splicing domains can be used for in vitro and in

87 utations at residues in the endonuclease and protein splicing domains.

88 icing, indicating that the N- and C-terminal protein-splicing domains can interact with sufficient af

89 The system utilizes a modified protein splicing element (intein) from Saccharomyces cer

90 The protein splicing element (intein) of the vacuolar ATPase

91 n derived from a naturally occurring, 43 kDa protein splicing element (intein) through a combination

92 has been facilitated by fusion to a modified protein splicing element (intein).

93 ein is fused to the C-terminus of a modified protein splicing element (intein).

94 f the intein fragments to yield a functional protein splicing element and for the protein splicing pr

95 agments were reconstituted into a functional protein splicing element by renaturation from 6 M urea.

96 nt fused through its C terminus to an intein protein splicing element.

97 ng involves the self-catalyzed excision of a protein-splicing element, the intein, from flanking poly

98 Protein splicing elements (inteins) are present in many

99 Split marker gene segments fused to protein splicing elements called "inteins" can be separa

100 two halves (or partners) of naturally split protein splicing elements called inteins, a novel thiol-

101 Inteins are protein splicing elements that mediate a self-catalytic

102 Inteins are protein splicing elements that mediate their excision fr

103 We have compiled a list of all the inteins (protein splicing elements) whose sequences have been pub

104 Protein splicing elements, or inteins, catalyze their ow

105 cing involves the self-catalyzed excision of protein splicing elements, or inteins, from flanking pol

106 nsive engineering of the naturally occurring protein splicing elements, termed inteins, has led to th

107 Protein splicing elements, termed inteins, provide a fer

108 Inteins are self-catalytic protein splicing elements.

109 Most protein-splicing elements (inteins) function both as cat

110 The discovery of inteins, which are protein-splicing elements, has stimulated interest for v

111 Inteins are protein-splicing elements, most of which contain conserv

112 that correspond to the endonuclease and the protein-splicing elements.

113 and ligation, without recourse to the use of protein-splicing elements.

114 The ability of inteins to promote protein splicing even when inserted into a foreign conte

115 n of protein phosphatase 1 (PP1) with the SR protein splicing factor (SRSF1) to understand the founda

116 n with the solution structure of the related protein splicing factor 1 (SF1) indicates that most aspe

117 We recently showed that the serine arginine protein splicing factor 2/alternative splicing factor (S

118 splicing enhancer (ESE) dependent on the SR protein splicing factor ASF/SF2 or to the creation of an

119 results not only indicate that loss of an SR protein splicing factor can induce cell cycle arrest and

120 SRp38 is an SR protein splicing factor that functions as a general repr

121 ASF/SF2 is an SR protein splicing factor that participates in constitutiv

122 n for the proper subnuclear storage of an SR protein splicing factor.

123 The multifunctional protein, splicing factor, proline- and glutamine-rich (S

124 The top candidates include three host proteins splicing factor proline and glutamine rich (SFP

125 cognition and pairing is often influenced by protein splicing factors (SFs) that bind to splicing reg

126 clear ribonucleoprotein particle and Ser-Arg protein splicing factors and also with pre-mRNA splicing

127 d specifically influences the activity of SR protein splicing factors and, importantly, show that bot

128 ribonuclear protein and serine-arginine rich protein splicing factors during interphase.

129 ve biochemical properties consistent with SR protein splicing factors, and some, but not all, of the

130 lex A formation (pre-mRNA sequence elements, protein splicing factors, SF1/BBP and both subunits of U

131 We report here that two SR protein splicing factors, SRp20 and ASF/SF2, associate w

132 U1, U2, U4, U5, and U6) and dozens of other protein splicing factors.

133 cessing requires study of snRNAs, as well as protein splicing factors.

134 that phosphorylate serine-arginine-rich (SR) protein splicing factors.

135 Strikingly, SF2/ASF, one of the essential SR protein-splicing factors, causes a dose-dependent shift

136 SR proteins (splicing factors containing arginine-serine re

137 SR proteins (splicing factors containing arginine-serine re

138 -based evolutionary link between RNA binding proteins, splicing factors, and replication initiators o

139 genous factors and light differentiates this protein splicing from autocatalytic inteins, and may all

140 editing, drawing from split intein-mediated protein splicing, genetic code expansion, and endogenous

141 In the two decades since its discovery, protein splicing has been harnessed for the development

142 Intein-mediated protein splicing has found broad biotechnological applic

143 lso show that copper treatment inhibits Prp8 protein splicing in Cne.

144 Inhibition of protein splicing in Cryptococcus sp. interferes with act

145 t studies have been done on the mechanism of protein splicing in mesophiles.

146 speed, and dose dependence of ligand-induced protein splicing in murine NIH3T3 cells and in human HEK

147 xt facilitated the study of the mechanism of protein splicing in thermophiles.

148 naturally occurring proteins that carry out protein splicing in trans.

149 The occurrence of protein splicing in vertebrates has important implicatio

150 s have been shown to play important roles in protein splicing, including the most conserved "B-block"

151 arate components, could nevertheless mediate protein splicing, indicating that the N- and C-terminal

152 is presented, suggesting that metal-induced protein splicing inhibition would disturb function of bo

153 le as a high-throughput screening system for protein splicing inhibitors as potential antimycobacteri

154 In order to search for protein splicing inhibitors, which may attenuate protein

155 rotein splicing occurs in trans to assay for protein-splicing inhibitors, we discovered that low conc

156 e-5) methyltransferase (human DNMT1) using a protein splicing (intein) fusion partner in a baculoviru

157 talyzing changes in the structure of the RNA/protein splicing intermediate that promote the second st

158 Protein splicing involves the excision of an internal pr

159 Protein splicing involves the excision of an intervening

160 Protein splicing involves the excision of an intervening

161 Protein splicing involves the self-catalyzed excision of

162 Protein splicing involves the self-catalyzed excision of

163 Protein splicing involves the self-catalyzed excision of

164 Protein splicing involves the self-catalyzed formation o

165 This process, known as protein splicing, involves multiple chemical steps that

166 This is the first example of protein splicing involving a synthetic intein fragment a

167 Protein splicing is a form of posttranslational processi

168 Protein splicing is a naturally occurring process in whi

169 Protein splicing is a naturally occurring process in whi

170 Protein splicing is a post-translational process by whic

171 Protein splicing is a posttranslational autocatalytic pr

172 Protein splicing is a posttranslational modification whe

173 Conditional protein splicing is a powerful biotechnological tool tha

174 Protein splicing is a precise autocatalytic process in w

175 Protein splicing is a precise self-catalyzed process in

176 Protein splicing is a robust multistep posttranslational

177 Protein splicing is a self-catalytic process in which an

178 Protein splicing is a self-catalyzed and spontaneous pos

179 Protein splicing is an autocatalytic reaction where an i

180 Protein splicing is an intricate self-catalyzed protein

181 survive in the presence of inducer only when protein splicing is blocked.

182 We showed that protein splicing is necessary for recombination activity

183 iled understanding of their participation in protein splicing is needed.

184 optimal conditions (pH 6.5 and 20 degrees C) protein splicing is significantly slower than GFP chromo

185 Variations in the intein-mediated protein splicing mechanism are becoming more apparent as

186 newly identified conserved residues, a novel protein splicing mechanism that includes a second branch

187 between critical active-site residues in the protein splicing mechanism, thereby facilitating biotech

188 eins splice efficiently using an alternative protein splicing mechanism.

189 Protein splicing mediated by inteins is a self-processiv

190 mutations or inhibitors that interfere with protein splicing mediated by the RecA intein of Mycobact

191 recently developed in vitro systems in which protein splicing occurs in trans to assay for protein-sp

192 Protein splicing occurs through acid-base catalysis in w

193 The ability to control protein splicing of a thermophilic intein by temperature

194 om Saccharomyces cerevisiae, is generated by protein splicing of an intein, which is an internal poly

195 in splicing and we have used it to show that protein splicing of the RecA intein is compatible with a

196 Protein splicing of the Saccharomyces cerevisiae vacuola

197 ns), mobile genetic elements removed through protein splicing, often interrupt proteins required for

198 The intein facilitates in vitro protein splicing only at temperatures above 30 degrees C

199 nable picture of the basic chemical steps in protein splicing, our knowledge of how these are catalyz

200 perform the initial reaction of the standard protein splicing pathway to yield the requisite N-termin

201 ions were used to block various steps in the protein splicing pathway, allowing each isolated step to

202 ed a single protein that splices by the Ala1 protein splicing pathway, with splicing dependent on adj

203 al and Ssp DnaE intein-specific steps in the protein splicing pathway.

204 een used to study the structure of an active protein splicing precursor, corresponding to an N-extein

205 ophiles at neutral pH than do oxygen esters, protein-splicing precursors in which the serine residue

206 g and allow structural studies of unmodified protein-splicing precursors.

207 Protein splicing proceeds through a four-step reaction b

208 ctional protein splicing element and for the protein splicing process per se.

209 ing endonuclease domain plays no role in the protein-splicing process and that the protein-splicing a

210 it and inverted" configuration such that the protein splicing product is a cyclic polypeptide consist

211 Understanding the mechanism of protein splicing provides a basis for protein engineerin

212 Intein-mediated protein splicing raises questions and creates opportunit

213 themselves out of precursor proteins by the protein splicing reaction and have emerged as valuable p

214 rminus of most inteins initiates a four-step protein splicing reaction by forming a (thio)ester bond

215 urring intervening sequences that catalyze a protein splicing reaction resulting in intein excision a

216 homing endonucleases that is generated by a protein splicing reaction.

217 icing elements that mediate a self-catalytic protein splicing reaction.

218 milar strategy is utilized in self-catalyzed protein splicing reactions and in autoproteolytic activa

219 ot been identified, nor have intein-mediated protein splicing reactions been demonstrated, in plant c

220 SRp38 is an atypical SR protein splicing regulator.

221 Protein splicing results in the expression of two mature

222 intein, revealing structural homology among protein splicing sequences in eukaryotes, including the

223 oised to carry out the rate-limiting step in protein splicing, shedding light on how a nominally nonn

224 ng fusion protein was found to be capable of protein splicing similar to that of the parent intein.

225 g H2A.Z deposition using a steroid-inducible protein splicing strategy, we show that NFR establishmen

226 Using a split-intein protein-splicing strategy, we show that a functional TAL

227 activation of the cytotoxic CcdB protein by protein splicing, such that host cells survive in the pr

228 us to develop the first mesophilic in vitro protein splicing system as well as strategies for modula

229 The availability of such an in vitro protein splicing system opens the way for the exploratio

230 Here, we describe an ultrasound-responsive protein splicing system that enables spatiotemporal cont

231 tide synthesis (SPPS) or biosynthetically by protein splicing techniques.

232 By expanding the sonogenetic toolbox with protein splicing technologies, this study provides a pos

233 In this process known as protein splicing, the intein itself is not present in th

234 he domains of inteins that are essential for protein splicing, the intein sequence embedded in the re

235 vity in a ligand-dependent manner, we linked protein splicing to cell survival or fluorescence in Sac

236 g hybrid promoters and split intein-mediated protein splicing to integrate signals.

237 lly split intein permits rapid activation of protein splicing to yield a new protein product.

238 sufficient affinity and specificity to allow protein-splicing to occur in trans.

239 s of backbone-cyclized Crp4 using a modified protein splicing unit or intein.

240 In this study, protein splicing was controlled by splitting precursor p

241 The level of protein splicing was dose dependent and could be competi

242 Two important aspects of protein splicing were investigated by employing the tran

243 Inteins mediate protein splicing, which has found many applications in b

244 tion cascade that integrates intein-mediated protein splicing with enzyme-mediated peptide ligation.

245 or controlling a post-translational process, protein splicing, with light.