ライフサイエンスコーパス: protein splicing

1 s and as tools for studying the mechanism of protein splicing.

2 e CcdB toxin unless the intein is excised by protein splicing.

3 sis of structural and mechanistic aspects of protein splicing.

4 s facilitated the study of the modulation of protein splicing.

5 published and unpublished information about protein splicing.

6 nserved residues in block F are required for protein splicing.

7 trapped in the branched intermediate step in protein splicing.

8 initely a close evolutionary relationship to protein splicing.

9 inally, we describe instances of conditional protein splicing.

10 nctional roles of some conserved residues in protein splicing.

11 tein structure and the unusual enzymology of protein splicing.

12 and two His residues that are implicated in protein splicing.

13 rearrangement constitutes the first step in protein splicing.

14 o an active conformation that promotes rapid protein splicing.

15 ndicating their potential functional role in protein splicing.

16 highly efficient Cre-reconstitution through protein splicing.

17 important role in coordinating the steps of protein splicing.

18 e in transesterification, the second step of protein splicing.

19 ribute to the higher optimal temperature for protein splicing.

20 ination mechanism for the first two steps of protein splicing.

21 romote the N-S acyl shift, the first step of protein splicing.

22 us of the intein, which are active sites for protein splicing.

23 as a dual role in the acid-base catalysis of protein splicing.

24 r intermediate formed during intein-mediated protein splicing.

25 n that prevent the second and third steps of protein splicing.

26 ius or higher, the intein mediates efficient protein splicing.

27 autocatalytically excise themselves through protein splicing.

28 e moderately improves the rate and extent of protein splicing.

29 kinetic analysis of the individual steps of protein splicing.

30 as able to reactivate the intein and trigger protein splicing.

31 rminal glutamine, is capable of facilitating protein splicing.

32 ng a nine-residue FGF-5 peptide generated by protein splicing.

33 ures of domains with single endonuclease and protein splicing active sites.

34 in the protein-splicing process and that the protein-splicing active center is confined to the N- and

35 We speculate that this protein splicing activity arose to post-translationally

36 Their protein splicing activity is essential for the host prot

37 th hyper- and hypophosphorylation inhibit SR protein splicing activity, repressing constitutive splic

38 137 residues may be the lower limit for full protein-splicing activity.

39 erminal splicing junctions, blocking in vivo protein splicing, allowed the miniprecursor to be purifi

40 Some of the reactions characteristic of protein splicing also occur in other forms of protein au

41 uld be useful in studies of the mechanism of protein splicing and allow structural studies of unmodif

42 ents (inteins) function both as catalysts of protein splicing and as homing endonucleases.

43 in, the renatured fusion protein can undergo protein splicing and chromophore formation.

44 First, we demonstrated that both protein splicing and cleavage at the N-terminal splice j

45 1 substitutions attenuated the initiation of protein splicing and enabled us to demonstrate in vitro

46 The PI-SceI protein splicing and endonucleolytic active sites are se

47 eI has a bipartite domain structure, and the protein splicing and endonucleolytic reactions are catal

48 utamine in the C-terminal position, prevents protein splicing and facilitates cleavage at the intein

49 ell as strategies for modulating the rate of protein splicing and for converting the splicing reactio

50 ) of Saccharomyces cerevisiae catalyzes both protein splicing and site-specific DNA cleavage.

51 hat propagates its mobile gene by catalyzing protein splicing and site-specific DNA double-strand cle

52 e junction residues are directly involved in protein splicing and the central dodecapeptide motifs ar

53 nput, enabling enhanced genetic control over protein splicing and the potential creation of splicing-

54 Hedgehog/INTein (HINT) domains catalyzing protein splicing and their nested HEN domains are though

55 this alignment (Cfa) demonstrates both rapid protein splicing and unprecedented thermal and chaotropi

56 screening system detects even low levels of protein splicing and we have used it to show that protei

57 acid Mtu recA intein consists of independent protein-splicing and endonuclease domains.

58 The disulfide bond inhibits protein splicing, and splicing can be induced by reducin

59 an undecapeptide spacer) was able to promote protein splicing as efficiently as the wild-type intein,

60 protein splicing by zinc ion, a fluorometric protein splicing assay was developed in which the denatu

61 es an in vitro fluorometric assay system for protein splicing based on the RecA intein of Mycobacteri

62 hat is not only catalytically sufficient for protein splicing but also structurally independent from

63 at the -1 position had no effect on overall protein splicing but could lead to significant accumulat

64 None affects protein splicing, but one, a R417Q substitution, account

65 artate (D422) coordinates different steps in protein splicing, but the precise mechanism is unclear.

66 be an anomaly found in only a few organisms, protein splicing by inteins has since been observed in m

67 ein splicing inhibitors, which may attenuate protein splicing by less than an order of magnitude, we

68 fragments, which could be induced to undergo protein splicing by reduction of the disulfide bond.

69 ng advantage of the reversible inhibition of protein splicing by zinc ion, a fluorometric protein spl

70 Protein splicing can be modulated by mutation and conver

71 We demonstrate for the first time that protein splicing can proceed efficiently after the remov

72 When diluted into appropriate buffers, protein splicing could be initiated by the addition of a

73 n this report, we show that this conditional protein splicing (CPS) system can be used in mammalian c

74 vage site region of the substrate, while the protein splicing domain (domain I) interacts with a dist

75 The majority of inteins are comprised of a protein splicing domain and a homing endonuclease domain

76 the codon for Arg94, which is located in the protein splicing domain and makes essential contacts to

77 Conversely, residues 91, 97, and 170 in the protein splicing domain are in close proximity to a dist

78 experimental evidence demonstrates that the protein splicing domain as well as the endonuclease doma

79 t of two functionally independent domains, a protein-splicing domain and an endonuclease domain.

80 rrored by the functional independence of the protein-splicing domain, the entire endonuclease compone

81 These naturally occurring protein splicing domains can be used for in vitro and in

82 utations at residues in the endonuclease and protein splicing domains.

83 icing, indicating that the N- and C-terminal protein-splicing domains can interact with sufficient af

84 The system utilizes a modified protein splicing element (intein) from Saccharomyces cer

85 The protein splicing element (intein) of the vacuolar ATPase

86 n derived from a naturally occurring, 43 kDa protein splicing element (intein) through a combination

87 has been facilitated by fusion to a modified protein splicing element (intein).

88 ein is fused to the C-terminus of a modified protein splicing element (intein).

89 f the intein fragments to yield a functional protein splicing element and for the protein splicing pr

90 agments were reconstituted into a functional protein splicing element by renaturation from 6 M urea.

91 nt fused through its C terminus to an intein protein splicing element.

92 ng involves the self-catalyzed excision of a protein-splicing element, the intein, from flanking poly

93 Protein splicing elements (inteins) are present in many

94 two halves (or partners) of naturally split protein splicing elements called inteins, a novel thiol-

95 Inteins are protein splicing elements that mediate a self-catalytic

96 Inteins are protein splicing elements that mediate their excision fr

97 We have compiled a list of all the inteins (protein splicing elements) whose sequences have been pub

98 Protein splicing elements, or inteins, catalyze their ow

99 cing involves the self-catalyzed excision of protein splicing elements, or inteins, from flanking pol

100 nsive engineering of the naturally occurring protein splicing elements, termed inteins, has led to th

101 Protein splicing elements, termed inteins, provide a fer

102 Inteins are self-catalytic protein splicing elements.

103 Most protein-splicing elements (inteins) function both as cat

104 The discovery of inteins, which are protein-splicing elements, has stimulated interest for v

105 Inteins are protein-splicing elements, most of which contain conserv

106 that correspond to the endonuclease and the protein-splicing elements.

107 and ligation, without recourse to the use of protein-splicing elements.

108 The ability of inteins to promote protein splicing even when inserted into a foreign conte

109 n of protein phosphatase 1 (PP1) with the SR protein splicing factor (SRSF1) to understand the founda

110 n with the solution structure of the related protein splicing factor 1 (SF1) indicates that most aspe

111 We recently showed that the serine arginine protein splicing factor 2/alternative splicing factor (S

112 splicing enhancer (ESE) dependent on the SR protein splicing factor ASF/SF2 or to the creation of an

113 results not only indicate that loss of an SR protein splicing factor can induce cell cycle arrest and

114 SRp38 is an SR protein splicing factor that functions as a general repr

115 ASF/SF2 is an SR protein splicing factor that participates in constitutiv

116 n for the proper subnuclear storage of an SR protein splicing factor.

117 The top candidates include three host proteins splicing factor proline and glutamine rich (SFP

118 clear ribonucleoprotein particle and Ser-Arg protein splicing factors and also with pre-mRNA splicing

119 d specifically influences the activity of SR protein splicing factors and, importantly, show that bot

120 ribonuclear protein and serine-arginine rich protein splicing factors during interphase.

121 ve biochemical properties consistent with SR protein splicing factors, and some, but not all, of the

122 lex A formation (pre-mRNA sequence elements, protein splicing factors, SF1/BBP and both subunits of U

123 We report here that two SR protein splicing factors, SRp20 and ASF/SF2, associate w

124 that phosphorylate serine-arginine-rich (SR) protein splicing factors.

125 Strikingly, SF2/ASF, one of the essential SR protein-splicing factors, causes a dose-dependent shift

126 SR proteins (splicing factors containing arginine-serine re

127 SR proteins (splicing factors containing arginine-serine re

128 -based evolutionary link between RNA binding proteins, splicing factors, and replication initiators o

129 genous factors and light differentiates this protein splicing from autocatalytic inteins, and may all

130 In the two decades since its discovery, protein splicing has been harnessed for the development

131 Intein-mediated protein splicing has found broad biotechnological applic

132 t studies have been done on the mechanism of protein splicing in mesophiles.

133 speed, and dose dependence of ligand-induced protein splicing in murine NIH3T3 cells and in human HEK

134 xt facilitated the study of the mechanism of protein splicing in thermophiles.

135 naturally occurring proteins that carry out protein splicing in trans.

136 The occurrence of protein splicing in vertebrates has important implicatio

137 s have been shown to play important roles in protein splicing, including the most conserved "B-block"

138 arate components, could nevertheless mediate protein splicing, indicating that the N- and C-terminal

139 le as a high-throughput screening system for protein splicing inhibitors as potential antimycobacteri

140 In order to search for protein splicing inhibitors, which may attenuate protein

141 rotein splicing occurs in trans to assay for protein-splicing inhibitors, we discovered that low conc

142 e-5) methyltransferase (human DNMT1) using a protein splicing (intein) fusion partner in a baculoviru

143 talyzing changes in the structure of the RNA/protein splicing intermediate that promote the second st

144 Protein splicing involves the excision of an internal pr

145 Protein splicing involves the excision of an intervening

146 Protein splicing involves the excision of an intervening

147 Protein splicing involves the self-catalyzed excision of

148 Protein splicing involves the self-catalyzed excision of

149 Protein splicing involves the self-catalyzed excision of

150 Protein splicing involves the self-catalyzed formation o

151 This process, known as protein splicing, involves multiple chemical steps that

152 This is the first example of protein splicing involving a synthetic intein fragment a

153 Protein splicing is a form of posttranslational processi

154 Protein splicing is a naturally occurring process in whi

155 Protein splicing is a naturally occurring process in whi

156 Protein splicing is a posttranslational autocatalytic pr

157 Protein splicing is a posttranslational modification whe

158 Conditional protein splicing is a powerful biotechnological tool tha

159 Protein splicing is a precise autocatalytic process in w

160 Protein splicing is a precise self-catalyzed process in

161 Protein splicing is a robust multistep posttranslational

162 Protein splicing is a self-catalytic process in which an

163 Protein splicing is a self-catalyzed and spontaneous pos

164 Protein splicing is an autocatalytic reaction where an i

165 Protein splicing is an intricate self-catalyzed protein

166 survive in the presence of inducer only when protein splicing is blocked.

167 iled understanding of their participation in protein splicing is needed.

168 optimal conditions (pH 6.5 and 20 degrees C) protein splicing is significantly slower than GFP chromo

169 Variations in the intein-mediated protein splicing mechanism are becoming more apparent as

170 newly identified conserved residues, a novel protein splicing mechanism that includes a second branch

171 between critical active-site residues in the protein splicing mechanism, thereby facilitating biotech

172 eins splice efficiently using an alternative protein splicing mechanism.

173 Protein splicing mediated by inteins is a self-processiv

174 mutations or inhibitors that interfere with protein splicing mediated by the RecA intein of Mycobact

175 recently developed in vitro systems in which protein splicing occurs in trans to assay for protein-sp

176 Protein splicing occurs through acid-base catalysis in w

177 The ability to control protein splicing of a thermophilic intein by temperature

178 om Saccharomyces cerevisiae, is generated by protein splicing of an intein, which is an internal poly

179 in splicing and we have used it to show that protein splicing of the RecA intein is compatible with a

180 Protein splicing of the Saccharomyces cerevisiae vacuola

181 The intein facilitates in vitro protein splicing only at temperatures above 30 degrees C

182 nable picture of the basic chemical steps in protein splicing, our knowledge of how these are catalyz

183 perform the initial reaction of the standard protein splicing pathway to yield the requisite N-termin

184 ions were used to block various steps in the protein splicing pathway, allowing each isolated step to

185 ed a single protein that splices by the Ala1 protein splicing pathway, with splicing dependent on adj

186 al and Ssp DnaE intein-specific steps in the protein splicing pathway.

187 een used to study the structure of an active protein splicing precursor, corresponding to an N-extein

188 ophiles at neutral pH than do oxygen esters, protein-splicing precursors in which the serine residue

189 g and allow structural studies of unmodified protein-splicing precursors.

190 Protein splicing proceeds through a four-step reaction b

191 ctional protein splicing element and for the protein splicing process per se.

192 ing endonuclease domain plays no role in the protein-splicing process and that the protein-splicing a

193 it and inverted" configuration such that the protein splicing product is a cyclic polypeptide consist

194 Understanding the mechanism of protein splicing provides a basis for protein engineerin

195 Intein-mediated protein splicing raises questions and creates opportunit

196 themselves out of precursor proteins by the protein splicing reaction and have emerged as valuable p

197 rminus of most inteins initiates a four-step protein splicing reaction by forming a (thio)ester bond

198 urring intervening sequences that catalyze a protein splicing reaction resulting in intein excision a

199 homing endonucleases that is generated by a protein splicing reaction.

200 icing elements that mediate a self-catalytic protein splicing reaction.

201 milar strategy is utilized in self-catalyzed protein splicing reactions and in autoproteolytic activa

202 ot been identified, nor have intein-mediated protein splicing reactions been demonstrated, in plant c

203 SRp38 is an atypical SR protein splicing regulator.

204 Protein splicing results in the expression of two mature

205 oised to carry out the rate-limiting step in protein splicing, shedding light on how a nominally nonn

206 ng fusion protein was found to be capable of protein splicing similar to that of the parent intein.

207 g H2A.Z deposition using a steroid-inducible protein splicing strategy, we show that NFR establishmen

208 Using a split-intein protein-splicing strategy, we show that a functional TAL

209 activation of the cytotoxic CcdB protein by protein splicing, such that host cells survive in the pr

210 us to develop the first mesophilic in vitro protein splicing system as well as strategies for modula

211 The availability of such an in vitro protein splicing system opens the way for the exploratio

212 tide synthesis (SPPS) or biosynthetically by protein splicing techniques.

213 he domains of inteins that are essential for protein splicing, the intein sequence embedded in the re

214 vity in a ligand-dependent manner, we linked protein splicing to cell survival or fluorescence in Sac

215 g hybrid promoters and split intein-mediated protein splicing to integrate signals.

216 lly split intein permits rapid activation of protein splicing to yield a new protein product.

217 sufficient affinity and specificity to allow protein-splicing to occur in trans.

218 s of backbone-cyclized Crp4 using a modified protein splicing unit or intein.

219 In this study, protein splicing was controlled by splitting precursor p

220 The level of protein splicing was dose dependent and could be competi

221 Two important aspects of protein splicing were investigated by employing the tran

222 Inteins mediate protein splicing, which has found many applications in b

223 or controlling a post-translational process, protein splicing, with light.