ライフサイエンスコーパス: protein unfolding

1 the disruption of the hydrophobic core upon protein unfolding.

2 uire ATP hydrolysis-dependent, ClpX-mediated protein unfolding.

3 by fiber alignment and bundling after forced protein unfolding.

4 and repacking of apolar lipids but no global protein unfolding.

5 +)-binding sites, and prevents a substantial protein unfolding.

6 cific coordinates to elucidate mechanisms of protein unfolding.

7 P-hydrolysis rates, and facilitate efficient protein unfolding.

8 times to describe the stochastic dynamics of protein unfolding.

9 ut corresponded with the amount of substrate protein unfolding.

10 egulation and protection from stress-induced protein unfolding.

11 anner independent of the amount of substrate protein unfolding.

12 ical denaturation resulted in more extensive protein unfolding.

13 ology and protein conformation but no global protein unfolding.

14 mplexes by up to 20 degrees C and decelerate protein unfolding.

15 of energy barriers provide the mechanism for protein unfolding.

16 change in proteins by selenium could lead to protein unfolding.

17 or FMN binding and increases the barrier for protein unfolding.

18 tein interactions and reveal low-temperature protein unfolding.

19 enaturants (e.g., 8 M urea) needed to ensure protein unfolding.

20 is in contrast to the positive DeltaC(p) for protein unfolding.

21 e determination of heat capacity changes for protein unfolding.

22 ion of detergents from membrane proteins and protein unfolding.

23 hanges in water accessible surface area upon protein unfolding.

24 nical stretching force required for forceful protein unfolding.

25 ld make activation of hsp genes dependent on protein unfolding.

26 er, the approach commonly taken in models of protein unfolding.

27 ular determinants to the volume changes upon protein unfolding.

28 L subunits directly contributes to substrate protein unfolding.

29 from the free energy change for heat-induced protein unfolding.

30 rophilic agents, causing Zn(2+) ejection and protein unfolding.

31 face studies, such as membrane processes and protein unfolding.

32 machines couple ATP hydrolysis to mechanical protein unfolding.

33 ccurs in the fibrin clot as a part of forced protein unfolding.

34 effect of the CL surface and the large-scale protein unfolding.

35 nisms against oxidative stress that leads to protein unfolding.

36 rom oxidative stress that was accompanied by protein unfolding.

37 tide structures that vary in their degree of protein unfolding.

38 A/II frequencies were shown to be related to protein unfolding.

39 ciation can be separated from the process of protein unfolding.

40 and obtain force-dependent rate constants of protein unfolding.

41 ed pulse proteolysis as a probe for membrane protein unfolding.

42 sis is a quantitative tool to probe membrane protein unfolding.

43 ing similarities between interconversion and protein unfolding.

44 nregulation of metabolism, and prevention of protein unfolding.

45 nerable to stressful conditions that lead to protein unfolding, a potential contributor to the retina

46 has long been held that agents that promote protein unfolding act specifically on the denatured stat

47 Hsp70, which restricts the potentially toxic protein unfolding activity of Hsp104 to the disaggregati

48 of oxidative protein folding but also has a protein unfolding activity.

49 ondary and/or tertiary structure, leading to protein unfolding, aggregation and loss of function.

50 The DeltaCp for protein unfolding agrees with DeltaCp values for solvati

51 s contributions to the change in volume upon protein unfolding allowed us to put forth the hypothesis

52 ion of newly exposed atoms with solvent upon protein unfolding also contribute to the pressure unfold

53 ndent chaperone systems, making ROS-mediated protein unfolding and aggregation a potentially very cha

54 pt alternative conformations (such as during protein unfolding and aggregation) presents numerous cha

55 re, we show that bile salts cause widespread protein unfolding and aggregation, affecting many essent

56 s involved in protecting from stress-induced protein unfolding and aggregation, mitochondrial traffic

57 ergents can destabilize proteins, leading to protein unfolding and aggregation, or resulting in inact

58 bleach) treatment, which leads to widespread protein unfolding and aggregation.

59 tions leading to the loss of Zn, followed by protein unfolding and aggregation.

60 e use of single-molecule techniques to study protein unfolding and also provide insight into the type

61 d at 222 nm indicates close coupling between protein unfolding and an increase in the complex size an

62 Our analysis unites thermodynamics of protein unfolding and conformation-specific binding with

63 n and delivery to the proteasome followed by protein unfolding and disassembly of the multiubiquitin

64 force dependency of two different reactions, protein unfolding and disulfide reduction.

65 of ERp29, a PDI-like protein, regulates its protein unfolding and escort activities.

66 al of EX1 exchange to follow the kinetics of protein unfolding and folding is only beginning to be re

67 ng perspectives for early fault detection of protein unfolding and for obtaining mechanistic process

68 ortance since many diseases are initiated by protein unfolding and hence the propensity to aggregate

69 y describe the potentially diverse routes of protein unfolding and investigate how representative a s

70 the ferritin pores is a target for regulated protein unfolding and iron release.

71 Further additions of urea trigger complete protein unfolding and loss of all coppers.

72 rmational transition that does not result in protein unfolding and may in fact elude detection by tra

73 -protein ectodomain before triggering fusion protein unfolding and membrane fusion.

74 Thus, IRE1alpha senses protein unfolding and metabolic and immunological states

75 comparisons of the effects of temperature on protein unfolding and optimal enzymatic activity, we als

76 ard for recording full energetic profiles of protein unfolding and permitting data fitting based on u

77 This implies a direct link between transient protein unfolding and photosensory signal transduction.

78 s an in-line process analyzer for monitoring protein unfolding and protein-lyoprotectant hydrogen bon

79 Studies of protein unfolding and refolding may help us understand t

80 The kinetics of protein unfolding and refolding, as measured by changes

81 C; 0-15 min) on the relative molecular mass, protein unfolding and secondary structure of quinoa glob

82 is proposed that involves varying degrees of protein unfolding and subsequent binding to the membrane

83 Protein unfolding and the aqueous solubility of benzene

84 The contrast between protein unfolding and the hydration of nonpolar molecule

85 ATP hydrolysis by AAA+ ClpX hexamers powers protein unfolding and translocation during ClpXP degrada

86 the molecular mechanisms of enzyme catalyzed protein unfolding and translocation remain unanswered.

87 Protein unfolding and translocation through pores occurs

88 ed interspecific differences in urea-induced protein unfolding and ubiquitination and proteasomal act

89 uring the molecular elasticity of mechanical proteins, unfolding and refolding individual proteins, p

90 or dilution of the noncovalent bonds during protein unfolding, and identify the emergence of flexibl

91 ylation can accelerate protein folding, slow protein unfolding, and increase protein stability, but t

92 way, determined which barrier corresponds to protein unfolding, and mapped how the substrate protein

93 Increased temperatures accelerate protein unfolding, and we demonstrate that although AtPA

94 In 150 mM NaCl, two kinetic phases in HDL protein unfolding are observed: a faster phase with an a

95 present a possible model for how Hsp33 uses protein unfolding as a switch from self-recognition to h

96 modynamic stability for the CD-monitored H73 protein unfolding as compared to the unfolding of the WT

97 tural changes rather than requiring complete protein unfolding as do most domain-swapping proteins.

98 Irreversible protein unfolding at 37 degrees C was observed, and we p

99 are suitable for elucidating the details of protein unfolding at a minimum of computational expense.

100 ave a direct impact on the thermodynamics of protein unfolding at both high and low temperatures.

101 import machinery does not contribute to pre-protein unfolding at the organellar surface.

102 creasing the protein solubility and inducing protein unfolding before labeling with DNPH.

103 This salt-induced destabilization leads to protein unfolding below 100 degrees C, facilitating the

104 Mutation of these amino acids did not cause protein unfolding but destabilized PU.1-DNA binding.

105 ggest that the faster phase involves partial protein unfolding but no significant protein dissociatio

106 re expected to increase the rate not only of protein unfolding but protein folding as well.

107 iguous method of analysis of the kinetics of protein unfolding by a stretching force.

108 as denaturing osmolytes such as urea lead to protein unfolding by strongly binding to the surface.

109 ct observations of mechanical, force-induced protein unfolding by the ClpX unfoldase from E. coli, al

110 thout the need for new SOD1 synthesis or for protein unfolding by the major SSA cytosolic heat shock

111 bone provides the dominant driving force for protein unfolding by this denaturant, and the unfavorabl

112 bservations suggest that volume changes upon protein unfolding can be both positive and negative.

113 n and indicate that stimulus-induced partial protein unfolding can be used as a signaling mechanism.

114 surfactant binding to the protein and, thus, protein unfolding, can be tuned with light.

115 llular processes, including DNA replication, protein unfolding, cargo transport and membrane fusion.

116 allows L. infantum to deal successfully with protein unfolding conditions during the transition from

117 ion of Hsp33 confers resistance to oxidative protein unfolding conditions, we made use of Vibrio chol

118 th the means for surviving a range of acidic protein-unfolding conditions.

119 The program takes into account the protein-unfolding curve and generates propensities using

120 Comparison of urea-induced protein unfolding curves provide further evidence that d

121 during protein dehydration in the absence of protein unfolding (desired process course), whereas cons

122 lizes discoidal lipoproteins and accelerates protein unfolding, dissociation, and lipoprotein fusion.

123 we have identified a physiological source of protein-unfolding disulfide stress conditions in bacteri

124 provides a useful model system for study of protein unfolding due to its accessibility with several

125 occurs abruptly with no evidence of partial protein unfolding during detachment.

126 Thus, protein unfolding during import resembles mechanical unf

127 d (2D IR) spectroscopy is used as a probe of protein unfolding dynamics in a direct comparison of fas

128 oal of predicting urea-dependent cooperative protein unfolding energetics at the level of individual

129 ment is achieved between the 1995 values for protein unfolding enthalpies in vacuum given by Lazaridi

130 The rate of protein unfolding events increased with increasing anion

131 lent Strep-Tactin is generally applicable to protein unfolding experiments.

132 , who measured differential free energies of protein unfolding for site-specific mutants in which Ala

133 tly, long extrapolations of helical membrane protein unfolding free energies must be treated with cau

134 re employed to dissect the thermodynamics of protein unfolding from the two folded states.

135 hat dimerization of ERp29 is crucial for its protein unfolding function.

136 Unlike CD, A(230) allowed us to monitor protein unfolding in a 96-well microtiter plate with a U

137 data and their comparison with the reported protein unfolding in a Paul trap imply that at least som

138 tudied, since HSP90 has been linked with p53 protein unfolding in human cancers.

139 ting may allow the investigation of membrane protein unfolding in situ without overexpression or puri

140 ular dichroism (CD) to measure the degree of protein unfolding in the absence and presence of potenti

141 ds to assay the kinetics of enzyme-catalyzed protein unfolding in the absence of proteolysis have bee

142 Measurements of protein unfolding in the absence of solvent, when combin

143 freeze-drying, there was a large increase in protein unfolding in the dried solid and in protein aggr

144 igargin and tunicamycin, agents that promote protein unfolding in the endoplasmic reticulum (ER).

145 ogen-rich environment are coupled to limited protein unfolding in the ER, thereby activating the UPR.

146 the first detailed mechanism for multidomain protein unfolding in the gas phase, and highlights key s

147 ster effects on protein stability leading to protein unfolding in the heated ESI droplet.

148 ed as a fast and reliable method of tracking protein unfolding in the presence of a chemical denatura

149 g calorimetry (DSC) curves are simulated for protein unfolding in the presence of ligands binding to

150 al unfolding have been carried out; however, protein unfolding in the presence of the widely used den

151 es C), suggesting significant involvement of protein unfolding in the reaction pathway.

152 ar ratios of HOCl to protein cause oxidative protein unfolding in vitro and target thermolabile prote

153 photolabeling approach to monitor changes in protein unfolding in vivo in skeletal muscle proteins in

154 ation into a monomeric intermediate precedes protein unfolding, in contrast to the typical two-state

155 est that the GroEL chaperonin may facilitate protein unfolding indirectly by interacting with interme

156 f a more random coil structure, resulting in protein unfolding, inhibition of PDI reductase and isome

157 pontaneous membrane curvature and subsequent protein unfolding interactions.

158 Structural characterization of protein unfolding intermediates, which until recently we

159 Protein unfolding is a key step in several cellular proc

160 The study suggested that protein unfolding is a more important phenomenon than so

161 rstanding the directionality and sequence of protein unfolding is crucial to elucidate the underlying

162 At high CR concentration, protein unfolding is favored over aggregation, and fibri

163 Protein unfolding is induced through collisional activat

164 These findings suggest that protein unfolding is not coupled to the adhesive functio

165 This indicated that pre-protein unfolding is not rate-limiting for import into m

166 nteraction of GB with the surface exposed in protein unfolding is predicted to exceed unity, which ex

167 This model for protein unfolding is supported by a range of recent expe

168 npolar side chains; what drives urea-induced protein unfolding is the large favorable interaction of

169 Protein unfolding is the most severe when the nanopartic

170 ely abolished in the unfolded states of both proteins, unfolding is completely reversible for HiGlpG

171 In addition, they show less urea-induced protein unfolding, less protein ubiquitination, and high

172 In this assay, ClpX is a more efficient protein-unfolding machine than ClpA both kinetically and

173 receptors, deubiquitinating enzymes and the protein unfolding machinery at subnanometre resolution,

174 he injected protein, indicating that partial protein unfolding may be a requirement for transport.

175 oxidized native alpha2M suggest that partial protein unfolding may be the most likely mechanism.

176 Elasticity created by some type of protein unfolding may be the only viable explanation con

177 Studies of protein unfolding mechanisms are critical for understand

178 s for applications including DNA sequencing, protein unfolding, micro-RNA detection, label-free detec

179 shear stress field above the critical value, protein unfolding occurs and vWF is observed in extended

180 Protein unfolding occurs at both low and high temperatur

181 Protein unfolding occurs when the balance of forces betw

182 These fits yielded a DeltaC(p) of protein unfolding of 826 +/- 23 cal mol(-)(1) K(-)(1) an

183 izing effects on proteins in vitro and cause protein unfolding of different subsets of proteins in vi

184 proteolytic activity of ClpP, ClpA catalyzes protein unfolding of proteins that display target sequen

185 recognition signals, catalyze ATP-dependent protein unfolding of the substrate, and when in complexe

186 the HBV capsids without unfolding the capsid protein; unfolding of dimer only occurred at higher dena

187 Protein unfolding on a fast time scale (milliseconds-min

188 tribution to the free energy barrier for the protein unfolding on the disk, DeltaG > or = 17 kcal/mol

189 activation of cyt c did not require complete protein unfolding or breakage of the Fe-S(Met80) bond.

190 lationship exists between the free energy of protein unfolding or loss of quaternary structure and th

191 s still undefined: does urea actively induce protein unfolding or passively stabilize the unfolded st

192 s responsive genes, a protective response to protein unfolding or protein damage resulting from cellu

193 lysis and proteolysis, possibly blocking the protein unfolding or remodeling step essential for degra

194 t affect osteogenesis, adipogenesis, surface-protein unfolding or underlying substrate deformations.

195 ze, while the slower phase involves complete protein unfolding, partial protein dissociation, and HDL

196 shown to be an exciting approach to discern protein unfolding pathways for conformationally diverse

197 ions (ATD), which is attributed to extensive protein unfolding, prior to ejection of a highly charged

198 In general, for these three proteins protein unfolding proceeded via expansion of the core an

199 o eukaryotic SOD2 molecules is driven by the protein unfolding process associated with mitochondrial

200 Dissecting a protein unfolding process into individual steps can prov

201 surface, which then triggers the successive protein unfolding process.

202 by oxidative stress conditions that lead to protein unfolding, protects cells against oxidative prot

203 t capacity changes (DeltaCp) often accompany protein unfolding, protein binding, and specific DNA-lig

204 These in vitro results provide support for protein unfolding/protein aggregation models for catarac

205 n compared with fluorescence measurements of protein unfolding rates, we find data that suggest a lin

206 ides a clear example of kinetic control in a protein unfolding reaction.

207 ular motor mechanics, biopolymer properties, protein unfolding, receptor-ligand interactions, and mor

208 tein fractions are resistant to urea-induced protein unfolding relative to the level of unfolding det

209 uplet that is maintained below 343 K, before protein unfolding replaces it by a rather strong positiv

210 ses to envelope stress; demonstrate that the protein-unfolding response, even under catastrophic cond

211 A biochemical probe for protein unfolding reveals that ClpX also unfolds MuA sub

212 t decrease, showing a specific and sensitive protein unfolding signature.

213 Under conditions promoting protein unfolding, small heat shock proteins (sHsps) pre

214 here are two ATP-requiring steps: an initial protein unfolding step followed by translocation of the

215 optical tweezers, together with traditional protein unfolding studies to explore how perturbants com

216 ainst stress conditions that cause oxidative protein unfolding, such as treatment with bleach or expo

217 obic and, thus, inducing a greater degree of protein unfolding than the UV-light (cis) form.

218 of the surfactant causes a greater degree of protein unfolding than the UV-light form, providing a me

219 kbones, exposed to the solvent after partial protein unfolding that may lead to transient protein oli

220 y a stretching force between two points of a protein, unfolding the protein to an extended state.

221 In molecular dynamics simulations of protein unfolding, the pathway of one protein molecule i

222 opposite phenotypes, we use a combination of protein unfolding thermodynamics and atomic force micros

223 ainly based on the induction of myofibrillar protein unfolding thus facilitating the formation of the

224 ntitative predictions of volume changes upon protein unfolding, thus paving the way to proteome-wide

225 Neither transition involves substantial protein unfolding; thus, the transition heat originates

226 Hyperfluorescent intensity maxima during protein unfolding titrations are often taken as a sign f

227 Equilibrium protein unfolding titrations indicate that the donor and

228 fibrin's elasticity, ranging from reversible protein unfolding to rubber-like elasticity.

229 Given that protein unfolding trajectories are very complicated func

230 e, non-additive shift in the midpoint of the protein unfolding transition (Tm).

231 We have identified protein unfolding transition state (TS) ensembles from m

232 , positive correlation between the number of protein unfolding transitions observed in the gas phase

233 CD and light-scattering data suggest that protein unfolding triggers HDL fusion.

234 Simulations and experiments that monitor protein unfolding under denaturing conditions are common

235 g linear [Fe3S4](+) clusters released during protein unfolding under oxidative stress conditions and

236 nonexponential kinetics of single ubiquitin proteins unfolding under force.

237 orm what we believe to be the first study of protein unfolding via progressive localized compression.

238 d by over 30 degrees C; the heat capacity of protein unfolding was estimated from the dependence of c

239 In contrast, with CR at r = 8.8, protein unfolding was favored over aggregation.

240 The stability of MM-kappaI was reduced and protein unfolding was irreversible at pH 4.8, thus sugge

241 of pulse proteolysis as a probe for membrane protein unfolding, we chose Halobacterium halobium bacte

242 regate-based inhibitors proceeds via partial protein unfolding when bound to an aggregate particle.

243 riguing resemblance to phenomena observed in protein unfolding, where minimum rates are observed at t

244 cytochrome c with cardiolipin, resulting in protein unfolding, where the unfolded form interacts wit

245 he change in specific volume (DeltaV/V) upon protein unfolding, which defines the pressure dependence

246 xpulsion of water are both manifestations of protein unfolding, which is not apparent in other matrix

247 , site-specific structural information about protein unfolding, which is not resolved by standard, no

248 han dissociation to be independent of global protein unfolding, while analysis of the temperature dep

249 and likely to represent temperature-induced protein unfolding, while the slow phase is bimolecular a

250 ce-based thermostability assay that monitors protein unfolding with a thiol-reactive dye.

251 Protein unfolding with increased susceptibility to prote

252 teins are typically determined by monitoring protein unfolding with spectroscopic probes, such as cir

253 probing the temperature dependence of local protein unfolding within native proteins.