ライフサイエンスコーパス: protein-free

1 quirements show that the enzymes can utilize protein-free 23S rRNA as a substrate, but not the fully

2 s are extracted and the cleavage repeated on protein-free 23S rRNA.

3 t efficiently methylates protein-depleted or protein-free 23S rRNA.

4 aracteristic of these RNAs is an accessible, protein-free 5' or 3' end.

5 nt phospholipids, which were then trapped by protein-free acceptor vesicles.

6 mixtures and identification of the number of protein free and bound thiols have been demonstrated.

7 recombinant TSN initiated the decay of both protein-free and Argonaute 2-loaded miRNAs via endonucle

8 ts similar underlying molecular pathways for protein-free and neuronal SNARE-driven fusion.

9 ystem was developed for the determination of protein-free and total (free + bound forms) positron emi

10 ecular structures of Dicer and the Argonaute proteins, free and bound to small RNAs, have offered exc

11 d domains ("islands") that are separated by "protein-free" and cholesterol-low membrane.

12 in, albumin/fatty acid complex, lipoprotein, protein-free, and chylomicron fractions with no need of

13 We developed a fluorescence-based, protein-free assay for studying the cyclization of singl

14 talytic splint, we achieve room-temperature, protein-free assembly, offering a cost-effective alterna

15 insulin responses elicited by high-RS, whey protein-free bars were similar to those elicited from co

16 Although protein-free bicelles with such low q would likely show

17 lutinin, are compared with those studied for protein-free bilayers.

18 imilar to that observed in simulations with "protein-free" bilayers.

19 seq), which uses Tn5 transposase to sequence protein-free binding regions of the genome, can be combi

20 They received a single meal of protein-free biscuits and a drink containing zein (n = 8

21 se rates of these devices were determined in protein-free buffer or buffer containing 50% plasma prot

22 lacing the surrounding protein solution with protein-free buffer or by straightening of the molecule

23 The protein-free buffer samples injected into the syringes a

24 ately 20% when devices were transferred from protein-free buffer to buffer that contained protein (P:

25 cessfully replaced the culture medium with a protein-free buffer, as required for (89)Zr-oxine cell l

26 livers from female Sprague Dawley rats with protein-free buffered solution containing dimesna at con

27 formed cages surrounding different types of protein-free cage holes with similar cage holes spaced a

28 ing of hexamer and trimer units, surrounding protein-free cage holes.

29 n of two types of hexamer rings, surrounding protein-free cage holes.

30 Furthermore, the protein-free capsular bag system can be used to explore

31 rocesses (the term "enzyme-free" refers to a protein-free catalyst).

32 We found that protein-free cell extracts from H. salinarum provided a

33 Here we show that a protein-free complex of two snRNAs, U2 and U6, can bind

34 ng in SP ranged from 4% to 25%, resulting in protein-free concentrations >2-fold higher than BP.

35 hat RNA G-rich repetitive sequences can form protein-free condensates sustained by multimolecular G-q

36 binant protein, G-CSF-Tf, was harvested from protein-free, conditioned medium of transfected HEK293 c

37 n a gelatin gel HA growth system relative to protein-free controls.

38 -angle X-ray scattering shows that, like the protein-free cylinders, the cones are multilamellar with

39 rupts D loops mediated by yeast Rad51/Rad54; protein-free D loops or D loop mediated by bacterial Rec

40 the intermediate is probably not a canonical protein-free D-loop.

41 binding "light-up" RNA aptamers have enabled protein-free detection of RNA in cells.

42 tial for the development of next-generation, protein-free diagnostic tools, including digital assays

43 ethio-nine as tracers, after adjustment to a protein-free diet and how these rates compare with those

44 ven subjects were randomly assigned to a 5-d protein-free diet or a 5-d diet providing adequate nitro

45 1.4 and 24.7 +/- 3.6, respectively, with the protein-free diet; rates were significantly lower (3.9 +

46 isolation of regulatory elements to extract protein-free DNA (FAIRE) and the MNase-mediated purifica

47 otein complex with only limited stretches of protein-free DNA and (ii) CN + HP-induced breaks happen

48 A complex, which can further capture another protein-free DNA fragment.

49 A similar pattern of CPD formation in protein-free DNA loops suggests that DNA bending causes

50 h bound HU show much greater propensity than protein-free DNA to exist as negatively supercoiled topo

51 combines the known mechanical properties of protein-free DNA with the accumulating picture of chroma

52 In protein-free DNA, 8-oxodG adopts the syn conformation mo

53 P in a reduced mode at 60-80% of its rate on protein-free DNA.

54 resulting in a 6-fold increase compared with protein-free DNA.

55 robe strands affect the kinetic stability of protein-free double D-loop hybrids.

56 ation for native phospholipid flip-flop in a protein-free DPPC planar-supported lipid bilayer was det

57 agosome-like vesicles form at the surface of protein-free droplets in vitro through partial wetting.

58 plexes, facilitating repair of the resulting protein-free DSB by standard DNA repair pathways.

59 TOP2 protein, leading to the liberation of a protein-free DSB.

60 ired for the liberation of etoposide-induced protein-free DSBs and is therefore an important layer of

61 Alimentation with a protein-free, elemental diet led to an atrophic small in

62 y and NMR data reconstruction, and calculate protein free energies.

63 Each well in the protein free energy landscape (corresponding to folded,

64 ss along the populated folding routes on the protein free energy landscape.

65 ergy landscape in the vicinity of the folded protein free energy minimum.

66 ropic and energetic/enthalpic factors in the protein free energy regulates the details of this comple

67 hese studies demonstrate that in contrast to protein-free enterobacterial LPS, a similarly purified p

68 for the study of RNA dimers and trimers in a protein-free environment.

69 ent of macrophages with a pure TLR4 agonist (protein-free Escherichia coli (Ec) LPS) or with TLR2 ago

70 Here, we discovered that protein-free extracts of high fat-fed livers contained,

71 We show here that protein-free extracts of starved and high fat-fed livers

72 e (F-DKG), and their degradation products in protein-free extracts, by proton-decoupled 750-MHz (19)F

73 ed acyl donors, while acyl-ACP (acyl carrier protein), free fatty acids, or galactolipid-bound fatty

74 Protein-free fibril material was found to be highly acti

75 Although carbohydrate-rich, protein-free formula diets have been shown to elevate, a

76 ad sufficiently high solubility, high plasma protein free fraction, and favorable pharmacokinetics to

77 In the protein-free fraction and water-soluble proteins (WSP),

78 Aqueous pollen extracts, the protein-free fraction of Amb-APE, and the pollen-contain

79 eoxythreosone were the major products in the protein-free fraction, whereas in the WSP, 3-deoxythreos

80 tion by forming a soluble complex with the N protein free from cellular RNAs (designated N(0)).

81 r engineering a new generation of functional proteins free from natural evolution.

82 teractions resulted in constitutively active proteins, freed from autoinhibition and no longer influe

83 liposomes in multiple applications, but the protein-free fusion of those semisynthetic membranes is

84 used to compare the secondary structures of protein-free genomic fragments and the RNA in the virion

85 nriched in putative adhesins or adhesin-like proteins, free glutamic and aspartic acid, and choline g

86 ncy and breadth but no detectable binding to protein-free glycans.

87 ction for endogenous losses evaluated in the protein-free group.

88 These reactions do not occur if protein-free heme and Br(-) are co-incubated with H(2)O(

89 These reactions do not occur if protein-free heme and H(2)O(2) are co-incubated in aceta

90 O unit is bent more strongly in MbNO than in protein-free heme-NO complexes because of a combination

91 the pair of strands that are crossed in the protein-free Holliday junction.

92 and minimal essential medium and serum-free protein-free hybridoma medium (mammalian cell culture me

93 The structure of the human virus protein free in solution consists of an eight-stranded b

94 ly recognize S-adenosylhomocysteine (SAH) in protein-free in vitro assays, and confirmed that these R

95 A structural analysis of all three proteins free in buffer and bound to membranes indicates

96 complexes with those of the same constituent proteins free in solution.

97 e orthosteric binding pocket resembles the G protein-free 'inactive' receptor conformation, while the

98 rdiolipin promotes the formation of periodic protein-free inter-membrane contacts with rhombohedral s

99 multiple serotypes, but not with other host proteins, free iron, or heme.

100 e compared to the stacked X-structure of the protein-free junction in the presence of magnesium ions.

101 e of divalent metal ions, unlike that of the protein-free junction.

102 -amino acid derivative bind to the center of protein-free junctions and prevent their resolution eith

103 uses empirical values of mobilities of free protein, free ligand, and electroosmotic flow.

104 al dietary fatty acids from both albumin and protein-free lipid bilayers.

105 es by calculating the interfacial tension in protein-free lipid droplets, and in HDL and LDL particle

106 Protein-free lipid membranes with one-side Mn (2+)-bound

107 fusion and the role of opposite charges in a protein-free lipid system based on cationic liposomes (L

108 density lipoprotein (LDL) and modified LDL, protein-free lipid vesicles containing anionic phospholi

109 ein (LDL), exchangeable apolipoproteins, and protein-free lipid vesicles containing negatively charge

110 hus, equilibrium phase diagrams obtained for protein-free lipid/detergent mixtures would be misleadin

111 ow that AP-3 and clathrin are recruited onto protein-free liposomes and Golgi-enriched membranes by a

112 , dynactin-dependent vesicle transport using protein-free liposomes and soluble components from squid

113 ed in synaptic membranes can be generated on protein-free liposomes by incubation with cytosol, or wi

114 ilized ER vesicles under conditions in which protein-free liposomes containing ER lipids were inactiv

115 imide-sensitive fusion ATPase (NSF) can fuse protein-free liposomes containing substantial amounts of

116 pase activity was mediated by Drs2p, because protein-free liposomes or proteoliposomes reconstituted

117 ed release of entrapped 10 kDa dextrans from protein-free liposomes treated with Bax and cBid.

118 Protein-free liposomes were inactive.

119 Our data show that protein-free liposomes, after variable delay times, are

120 nase complex, promotes membrane tethering of protein-free liposomes, and enhances hemifusion and full

121 d the import and assembly pathway of Ugo1 in protein-free liposomes, mimicking the outer membrane pho

122 ARF.GTP also recruits AP-1 and clathrin onto protein-free liposomes.

123 ypsin or N-ethylmaleimide treatments or with protein-free liposomes.

124 rophages from C3H/OuJ mice were treated with protein-free LPS (100 ng/ml) or the LPS mimetic paclitax

125 ive C3H/HeJ macrophages failed to respond to protein-free LPS with an increase in steady-state AM mRN

126 D-2 enabled TLR2 to respond to nonactivating protein-free LPS, LPS mutants, or lipid A and enhanced T

127 Moreover, paclitaxel- and protein-free LPS-induced translocation of NF-kappaB was

128 appaB reporter gene in response to purified, protein-free LPS.

129 including the development of the serum- and protein-free media that now routinely support hybridoma

130 In protein-free media, both primary keratocytes and selecte

131 In serum- or protein-free media, CAD cells stop proliferating and ext

132 This trial also piloted the use of animal protein-free medium and a blood-bank-compatible closed s

133 incubating the cells in a chemically defined protein-free medium that provided a stable environment,

134 (MSCs) that produce EVs when incubated in a protein-free medium, preselecting the preparations of MS

135 antigens from promastigotes cultivated in a protein-free medium.

136 metabolic activity for more than 1 year in a protein-free medium.

137 at all stages of culture of capsular bags in protein-free medium.

138 lls to proliferate in serum-supplemented and protein-free medium.

139 proteins (flippases) since transport across protein-free membranes is negligible.

140 , fluidity, and lipid domain architecture of protein-free membranes reconstituted from IM and OM lipi

141 ies persisted in experiments using accessory protein-free membranes.

142 head domain bound to microtubule-associated protein-free microtubules.

143 e target protein is secreted directly into a protein-free mineral salt medium, and is relatively easy

144 We showed recently that the fusion of protein-free model lipid bilayers mimics the sequence of

145 distinguish as contributing to the fusion of protein-free model lipid bilayers.

146 We find that engulfment of protein-free model lipid vesicles is promoted by the pre

147 as been extensively investigated by exposing protein-free model membranes, either vesicles or planar

148 The protein-free model replicates many features of clinicall

149 To address this challenge, we propose that protein-free mRNA granule-like structures, named gene-en

150 ng zein (n = 8), WPI (n = 7), or no protein (protein free, n = 7).

151 tion and, importantly, resulted in a stable, protein-free nanoelectrospray signal.

152 n with LL-37 restored the uptake of "naked" (protein-free) NETs.

153 In contrast to existing methods (TCA soluble protein, free NH(2)-groups), the proposed SEC based meth

154 ombinase polymerase amplification (RPA) in a protein-free nucleic acid lateral flow (NALF) device for

155 the refined crystal structure of the MphR(A) protein free of erythromycin and that of the MphR(A) pro

156 GA and NaBH(3)CN for generating CML-enriched protein free of HCN, but a CML-only fortification model

157 used in the food industry as a novel dietary protein, free of fat and carbohydrate.

158 ral advantages for manufacturing therapeutic proteins free of zoonotic pathogens.

159 ganic nanoparticles to the molecule-specific proteins, free of agglomeration, nonspecific binding, or

160 While TSN-mediated degradation of either protein-free or AGO2-loaded miRNAs does not require the

161 erse transcriptase (RT) protein was removed (protein-free or PF-RC DNA).

162 abundant amounts of p21 devoid of associated proteins ("free" p21), the levels of which decrease as c

163 PARG, by generating protein-free PAR from poly-ADP ribosylated protein, make

164 n isolated perfused rat kidney model using a protein-free perfusate and perfusates containing bovine

165 However, in the protein-free perfusate, the EF of MAG3 was 64%, equal to

166 ng 7.5 or 2.5 g/dL bovine serum albumin or a protein-free perfusate.

167 of biomolecular condensates and can undergo protein-free phase separation in the presence of divalen

168 -flop), as fast as milliseconds, across both protein-free phospholipid bilayers and cell membranes.

169 known to diffuse (flip-flop) rapidly across protein-free phospholipid bilayers in their un-ionized f

170 ent partition coefficient similar to that of protein-free phospholipid bilayers; (ii) oleic acid rapi

171 ion leakage through transient water pores in protein-free phospholipid membranes.

172 opening and flickering during the fusion of protein-free phospholipid vesicles with planar phospholi

173 whether the intravenous administration of a protein-free, phospholipid-rich emulsion is an effective

174 The structure of the bilayer compared with a protein-free POPC control indicated hydrophobic matching

175 , and PchD were all able to utilize "carrier protein-free" pPant derivatives, the pattern of usage in

176 rvical epithelial cells were unresponsive to protein-free preparations of lipooligosaccharide from Ne

177 To verify that DMPO adducts of the protein free radicals had been formed, the reaction mixt

178 proteins resulting from the DMPO trapping of protein free radicals.

179 en proposed that involve the intermediacy of protein-free radicals, ferryl heme, nitrogen dioxide (NO

180 The protein-free regions are modeled individually as elastic

181 The protein-free RNA aptamer adopts a helical structure with

182 However, cryo-EM studies of protein-free RNA are in their early days.

183 Some PSs are partially present in protein-free RNA but others would need to refold from th

184 11 previously unknown 119- to 338-nucleotide protein-free RNA structures: full-length Tetrahymena rib

185 effective at stimulating ATPase activity as protein-free RNA.

186 wever, the physicochemical driving forces of protein-free, RNA-driven phase transitions remain unclea

187 Cryo-EM structure determination of protein-free RNAs has remained difficult with most attem

188 ce alignments and chemical probing data from protein-free RNAs were then used as pseudo-free energy c

189 some, indicating that it is a good model for protein-free rotationally phased bent DNA of the same cu

190 Use of this protein-free selective retrieval method eliminates the c

191 ated from Escherichia coli (2 d) and control protein-free SMALPS using E. coli polar lipid extract (1

192 bound to DNA spontaneously dissociates into protein-free solution.

193 significantly increase RecA nucleation onto protein-free ssDNA.

194 in-bound state, and the full agonist-bound G protein-free state.

195 contribution related to a higher content of proteins, free sugars, organic acids, PUFA and tocophero

196 B-like activity, with Exosurf, an artificial protein-free surfactant, and Survanta, a bovine protein-

197 ansfer will occur to some extent from HDL to protein-free synthetic membranes, one hypothesis is that

198 ile drop surfactometer experiments with both protein-free synthetic surfactant and hydrophobic protei

199 onsiveness to both re-extracted LPS and to a protein-free, synthetic preparation of lipid A.

200 complexes (PCAF complex, TFTC [TATA-binding-protein-free TAF(II)-containing complex], and STAGA [SPT

201 was significantly lower (P < 0.01) with the protein-free than with the SAA- or leucine-free diet.

202 ize is reached, in which the dynamics of Min proteins frees the cell membrane long enough to allow Ft

203 REs, leaving the remaining large parts of SM proteins free to execute their as yet unknown function a

204 LPL also promoted the internalization of protein-free triglyceride emulsions; lovastatin-treatmen

205 For its detection in protein-free ultrafiltrates or dialysates, a highly sens

206 erular ultrafiltrate to maintain essentially protein-free urine.

207 TCR adenovirus may offer a new efficacious, protein-free vaccination approach for the treatment of T

208 We developed a protein-free vaccine composed of aluminum hydroxide, mon

209 Together, these data show that this protein-free vaccine is a promising strategy to prevent

210 ive accord with expectations from studies of protein-free vesicle-vesicle fusion, the hemifusion rate

211 Efflux to protein-free vesicles prepared with 1-palmitoyl-2-oleoyl

212 ium from M2-containing vesicles, compared to protein-free vesicles, we conclude that M2 exhibits appr

213 ction of exogenous DGD promoted formation of protein-free viral genome, suggesting restoration of sev

214 complexes, some regions of the DNA remained protein-free while others, containing hRad52, interacted