ライフサイエンスコーパス: protein-free

1 quirements show that the enzymes can utilize protein-free 23S rRNA as a substrate, but not the fully

2 s are extracted and the cleavage repeated on protein-free 23S rRNA.

3 t efficiently methylates protein-depleted or protein-free 23S rRNA.

4 aracteristic of these RNAs is an accessible, protein-free 5' or 3' end.

5 nt phospholipids, which were then trapped by protein-free acceptor vesicles.

6 mixtures and identification of the number of protein free and bound thiols have been demonstrated.

7 recombinant TSN initiated the decay of both protein-free and Argonaute 2-loaded miRNAs via endonucle

8 ts similar underlying molecular pathways for protein-free and neuronal SNARE-driven fusion.

9 ystem was developed for the determination of protein-free and total (free + bound forms) positron emi

10 ecular structures of Dicer and the Argonaute proteins, free and bound to small RNAs, have offered exc

11 d domains ("islands") that are separated by "protein-free" and cholesterol-low membrane.

12 in, albumin/fatty acid complex, lipoprotein, protein-free, and chylomicron fractions with no need of

13 We developed a fluorescence-based, protein-free assay for studying the cyclization of singl

14 insulin responses elicited by high-RS, whey protein-free bars were similar to those elicited from co

15 Although protein-free bicelles with such low q would likely show

16 lutinin, are compared with those studied for protein-free bilayers.

17 imilar to that observed in simulations with "protein-free" bilayers.

18 se rates of these devices were determined in protein-free buffer or buffer containing 50% plasma prot

19 lacing the surrounding protein solution with protein-free buffer or by straightening of the molecule

20 ately 20% when devices were transferred from protein-free buffer to buffer that contained protein (P:

21 livers from female Sprague Dawley rats with protein-free buffered solution containing dimesna at con

22 formed cages surrounding different types of protein-free cage holes with similar cage holes spaced a

23 ing of hexamer and trimer units, surrounding protein-free cage holes.

24 n of two types of hexamer rings, surrounding protein-free cage holes.

25 Furthermore, the protein-free capsular bag system can be used to explore

26 rocesses (the term "enzyme-free" refers to a protein-free catalyst).

27 We found that protein-free cell extracts from H. salinarum provided a

28 Here we show that a protein-free complex of two snRNAs, U2 and U6, can bind

29 ng in SP ranged from 4% to 25%, resulting in protein-free concentrations >2-fold higher than BP.

30 binant protein, G-CSF-Tf, was harvested from protein-free, conditioned medium of transfected HEK293 c

31 n a gelatin gel HA growth system relative to protein-free controls.

32 -angle X-ray scattering shows that, like the protein-free cylinders, the cones are multilamellar with

33 rupts D loops mediated by yeast Rad51/Rad54; protein-free D loops or D loop mediated by bacterial Rec

34 the intermediate is probably not a canonical protein-free D-loop.

35 ethio-nine as tracers, after adjustment to a protein-free diet and how these rates compare with those

36 ven subjects were randomly assigned to a 5-d protein-free diet or a 5-d diet providing adequate nitro

37 1.4 and 24.7 +/- 3.6, respectively, with the protein-free diet; rates were significantly lower (3.9 +

38 isolation of regulatory elements to extract protein-free DNA (FAIRE) and the MNase-mediated purifica

39 A complex, which can further capture another protein-free DNA fragment.

40 A similar pattern of CPD formation in protein-free DNA loops suggests that DNA bending causes

41 h bound HU show much greater propensity than protein-free DNA to exist as negatively supercoiled topo

42 combines the known mechanical properties of protein-free DNA with the accumulating picture of chroma

43 P in a reduced mode at 60-80% of its rate on protein-free DNA.

44 resulting in a 6-fold increase compared with protein-free DNA.

45 robe strands affect the kinetic stability of protein-free double D-loop hybrids.

46 ation for native phospholipid flip-flop in a protein-free DPPC planar-supported lipid bilayer was det

47 y and NMR data reconstruction, and calculate protein free energies.

48 Each well in the protein free energy landscape (corresponding to folded,

49 ss along the populated folding routes on the protein free energy landscape.

50 ergy landscape in the vicinity of the folded protein free energy minimum.

51 ropic and energetic/enthalpic factors in the protein free energy regulates the details of this comple

52 hese studies demonstrate that in contrast to protein-free enterobacterial LPS, a similarly purified p

53 for the study of RNA dimers and trimers in a protein-free environment.

54 ent of macrophages with a pure TLR4 agonist (protein-free Escherichia coli (Ec) LPS) or with TLR2 ago

55 Here, we discovered that protein-free extracts of high fat-fed livers contained,

56 We show here that protein-free extracts of starved and high fat-fed livers

57 e (F-DKG), and their degradation products in protein-free extracts, by proton-decoupled 750-MHz (19)F

58 Protein-free fibril material was found to be highly acti

59 Although carbohydrate-rich, protein-free formula diets have been shown to elevate, a

60 ad sufficiently high solubility, high plasma protein free fraction, and favorable pharmacokinetics to

61 In the protein-free fraction and water-soluble proteins (WSP),

62 Aqueous pollen extracts, the protein-free fraction of Amb-APE, and the pollen-contain

63 eoxythreosone were the major products in the protein-free fraction, whereas in the WSP, 3-deoxythreos

64 tion by forming a soluble complex with the N protein free from cellular RNAs (designated N(0)).

65 r engineering a new generation of functional proteins free from natural evolution.

66 used to compare the secondary structures of protein-free genomic fragments and the RNA in the virion

67 ncy and breadth but no detectable binding to protein-free glycans.

68 These reactions do not occur if protein-free heme and Br(-) are co-incubated with H(2)O(

69 These reactions do not occur if protein-free heme and H(2)O(2) are co-incubated in aceta

70 O unit is bent more strongly in MbNO than in protein-free heme-NO complexes because of a combination

71 the pair of strands that are crossed in the protein-free Holliday junction.

72 and minimal essential medium and serum-free protein-free hybridoma medium (mammalian cell culture me

73 The structure of the human virus protein free in solution consists of an eight-stranded b

74 ly recognize S-adenosylhomocysteine (SAH) in protein-free in vitro assays, and confirmed that these R

75 A structural analysis of all three proteins free in buffer and bound to membranes indicates

76 complexes with those of the same constituent proteins free in solution.

77 e compared to the stacked X-structure of the protein-free junction in the presence of magnesium ions.

78 e of divalent metal ions, unlike that of the protein-free junction.

79 -amino acid derivative bind to the center of protein-free junctions and prevent their resolution eith

80 uses empirical values of mobilities of free protein, free ligand, and electroosmotic flow.

81 al dietary fatty acids from both albumin and protein-free lipid bilayers.

82 es by calculating the interfacial tension in protein-free lipid droplets, and in HDL and LDL particle

83 Protein-free lipid membranes with one-side Mn (2+)-bound

84 density lipoprotein (LDL) and modified LDL, protein-free lipid vesicles containing anionic phospholi

85 ein (LDL), exchangeable apolipoproteins, and protein-free lipid vesicles containing negatively charge

86 hus, equilibrium phase diagrams obtained for protein-free lipid/detergent mixtures would be misleadin

87 ow that AP-3 and clathrin are recruited onto protein-free liposomes and Golgi-enriched membranes by a

88 , dynactin-dependent vesicle transport using protein-free liposomes and soluble components from squid

89 ed in synaptic membranes can be generated on protein-free liposomes by incubation with cytosol, or wi

90 ilized ER vesicles under conditions in which protein-free liposomes containing ER lipids were inactiv

91 imide-sensitive fusion ATPase (NSF) can fuse protein-free liposomes containing substantial amounts of

92 pase activity was mediated by Drs2p, because protein-free liposomes or proteoliposomes reconstituted

93 ed release of entrapped 10 kDa dextrans from protein-free liposomes treated with Bax and cBid.

94 Protein-free liposomes were inactive.

95 Our data show that protein-free liposomes, after variable delay times, are

96 nase complex, promotes membrane tethering of protein-free liposomes, and enhances hemifusion and full

97 d the import and assembly pathway of Ugo1 in protein-free liposomes, mimicking the outer membrane pho

98 ARF.GTP also recruits AP-1 and clathrin onto protein-free liposomes.

99 ypsin or N-ethylmaleimide treatments or with protein-free liposomes.

100 rophages from C3H/OuJ mice were treated with protein-free LPS (100 ng/ml) or the LPS mimetic paclitax

101 ive C3H/HeJ macrophages failed to respond to protein-free LPS with an increase in steady-state AM mRN

102 D-2 enabled TLR2 to respond to nonactivating protein-free LPS, LPS mutants, or lipid A and enhanced T

103 Moreover, paclitaxel- and protein-free LPS-induced translocation of NF-kappaB was

104 appaB reporter gene in response to purified, protein-free LPS.

105 including the development of the serum- and protein-free media that now routinely support hybridoma

106 In protein-free media, both primary keratocytes and selecte

107 In serum- or protein-free media, CAD cells stop proliferating and ext

108 This trial also piloted the use of animal protein-free medium and a blood-bank-compatible closed s

109 incubating the cells in a chemically defined protein-free medium that provided a stable environment,

110 (MSCs) that produce EVs when incubated in a protein-free medium, preselecting the preparations of MS

111 antigens from promastigotes cultivated in a protein-free medium.

112 metabolic activity for more than 1 year in a protein-free medium.

113 at all stages of culture of capsular bags in protein-free medium.

114 lls to proliferate in serum-supplemented and protein-free medium.

115 proteins (flippases) since transport across protein-free membranes is negligible.

116 ies persisted in experiments using accessory protein-free membranes.

117 head domain bound to microtubule-associated protein-free microtubules.

118 e target protein is secreted directly into a protein-free mineral salt medium, and is relatively easy

119 We showed recently that the fusion of protein-free model lipid bilayers mimics the sequence of

120 distinguish as contributing to the fusion of protein-free model lipid bilayers.

121 We find that engulfment of protein-free model lipid vesicles is promoted by the pre

122 as been extensively investigated by exposing protein-free model membranes, either vesicles or planar

123 The protein-free model replicates many features of clinicall

124 tion and, importantly, resulted in a stable, protein-free nanoelectrospray signal.

125 the refined crystal structure of the MphR(A) protein free of erythromycin and that of the MphR(A) pro

126 ral advantages for manufacturing therapeutic proteins free of zoonotic pathogens.

127 ganic nanoparticles to the molecule-specific proteins, free of agglomeration, nonspecific binding, or

128 While TSN-mediated degradation of either protein-free or AGO2-loaded miRNAs does not require the

129 erse transcriptase (RT) protein was removed (protein-free or PF-RC DNA).

130 abundant amounts of p21 devoid of associated proteins ("free" p21), the levels of which decrease as c

131 PARG, by generating protein-free PAR from poly-ADP ribosylated protein, make

132 n isolated perfused rat kidney model using a protein-free perfusate and perfusates containing bovine

133 However, in the protein-free perfusate, the EF of MAG3 was 64%, equal to

134 ng 7.5 or 2.5 g/dL bovine serum albumin or a protein-free perfusate.

135 -flop), as fast as milliseconds, across both protein-free phospholipid bilayers and cell membranes.

136 known to diffuse (flip-flop) rapidly across protein-free phospholipid bilayers in their un-ionized f

137 ent partition coefficient similar to that of protein-free phospholipid bilayers; (ii) oleic acid rapi

138 ion leakage through transient water pores in protein-free phospholipid membranes.

139 opening and flickering during the fusion of protein-free phospholipid vesicles with planar phospholi

140 whether the intravenous administration of a protein-free, phospholipid-rich emulsion is an effective

141 The structure of the bilayer compared with a protein-free POPC control indicated hydrophobic matching

142 , and PchD were all able to utilize "carrier protein-free" pPant derivatives, the pattern of usage in

143 rvical epithelial cells were unresponsive to protein-free preparations of lipooligosaccharide from Ne

144 To verify that DMPO adducts of the protein free radicals had been formed, the reaction mixt

145 proteins resulting from the DMPO trapping of protein free radicals.

146 en proposed that involve the intermediacy of protein-free radicals, ferryl heme, nitrogen dioxide (NO

147 The protein-free regions are modeled individually as elastic

148 The protein-free RNA aptamer adopts a helical structure with

149 Some PSs are partially present in protein-free RNA but others would need to refold from th

150 effective at stimulating ATPase activity as protein-free RNA.

151 ce alignments and chemical probing data from protein-free RNAs were then used as pseudo-free energy c

152 some, indicating that it is a good model for protein-free rotationally phased bent DNA of the same cu

153 Use of this protein-free selective retrieval method eliminates the c

154 ated from Escherichia coli (2 d) and control protein-free SMALPS using E. coli polar lipid extract (1

155 bound to DNA spontaneously dissociates into protein-free solution.

156 significantly increase RecA nucleation onto protein-free ssDNA.

157 contribution related to a higher content of proteins, free sugars, organic acids, PUFA and tocophero

158 B-like activity, with Exosurf, an artificial protein-free surfactant, and Survanta, a bovine protein-

159 ansfer will occur to some extent from HDL to protein-free synthetic membranes, one hypothesis is that

160 onsiveness to both re-extracted LPS and to a protein-free, synthetic preparation of lipid A.

161 complexes (PCAF complex, TFTC [TATA-binding-protein-free TAF(II)-containing complex], and STAGA [SPT

162 was significantly lower (P < 0.01) with the protein-free than with the SAA- or leucine-free diet.

163 REs, leaving the remaining large parts of SM proteins free to execute their as yet unknown function a

164 LPL also promoted the internalization of protein-free triglyceride emulsions; lovastatin-treatmen

165 For its detection in protein-free ultrafiltrates or dialysates, a highly sens

166 erular ultrafiltrate to maintain essentially protein-free urine.

167 TCR adenovirus may offer a new efficacious, protein-free vaccination approach for the treatment of T

168 ive accord with expectations from studies of protein-free vesicle-vesicle fusion, the hemifusion rate

169 Efflux to protein-free vesicles prepared with 1-palmitoyl-2-oleoyl

170 ium from M2-containing vesicles, compared to protein-free vesicles, we conclude that M2 exhibits appr

171 ction of exogenous DGD promoted formation of protein-free viral genome, suggesting restoration of sev

172 complexes, some regions of the DNA remained protein-free while others, containing hRad52, interacted