ライフサイエンスコーパス: proteoliposome

1 escence correlation spectroscopy in a single proteoliposome.

2 ing the number of reconstituted channels per proteoliposome.

3 roteolytic degradation in the context of the proteoliposome.

4 ver-produced in E. coli and reconstituted in proteoliposomes.

5 tation and functionality during formation of proteoliposomes.

6 enhanced membrane fusion of SNARE-containing proteoliposomes.

7 e N-domain to anchor Vam7tm to reconstituted proteoliposomes.

8 by release of an H(+) on the interior of the proteoliposomes.

9 )(1/2)s were purified and reconstituted into proteoliposomes.

10 channel, thus forming a stationary phase of proteoliposomes.

11 lowering the phosphate concentration within proteoliposomes.

12 d orientation to be determined in unoriented proteoliposomes.

13 er and have reconstituted ATPase activity in proteoliposomes.

14 uld be inserted into yeast GET1/2-containing proteoliposomes.

15 ing the solvent within membrane vesicles and proteoliposomes.

16 tudies, the transporter was reconstituted in proteoliposomes.

17 LacY either in detergent or in reconstituted proteoliposomes.

18 but not Co(2+) or Cu(2+), into reconstituted proteoliposomes.

19 ATPase activity following reconstitution in proteoliposomes.

20 es also regulate the fusion of reconstituted proteoliposomes.

21 purified and functionally reconstituted into proteoliposomes.

22 on and reconstitution of this P4-ATPase into proteoliposomes.

23 floatation experiments with SNARE-containing proteoliposomes.

24 gella dysenteriae has been incorporated into proteoliposomes.

25 g protein-containing lipid vesicles known as proteoliposomes.

26 o of water permeability measurements on AQP0 proteoliposomes.

27 e efficiently taken up into P(i)-loaded G6PT-proteoliposomes.

28 the higher level of rhodopsin activity in RD proteoliposomes.

29 ling the lipid to protein ratio in the final proteoliposomes.

30 saying radioactive isotope uptake into these proteoliposomes.

31 d incorporating them together with IL-2 into proteoliposomes.

32 coli whole cells and GlpT reconstituted into proteoliposomes.

33 phenotype when they were reconstituted into proteoliposomes.

34 er complex both in detergent solution and in proteoliposomes.

35 purified and reconstituted into unilamellar proteoliposomes.

36 ity required the presence of AcrA within the proteoliposomes.

37 , we expressed DNC and reconstituted it into proteoliposomes.

38 retained transport activity in bacteria and proteoliposomes.

39 reconstituted the purified transporter into proteoliposomes.

40 aining single complexes up to densely packed proteoliposomes.

41 an inhibitor of GLUT5 fructose transport in proteoliposomes.

42 ng of reconstituted target and vesicle SNARE proteoliposomes.

43 emical synthesis of phospholipids to prepare proteoliposomes.

44 riggered sudden fusion of trans-SNARE paired proteoliposomes.

45 fluoroquinolone antibiotic, norfloxacin, in proteoliposomes.

46 by increasing mole fractions of DOPS in the proteoliposomes.

47 headgroup and fatty acyl composition of the proteoliposomes.

48 he conformation of MalFG in nanodiscs and in proteoliposomes.

49 and essentially the same in detergent or in proteoliposomes.

50 roteins can be reproduced with reconstituted proteoliposomes.

51 ies show that pH regulates calcium influx in proteoliposomes.

52 cessary and sufficient to transport FHA into proteoliposomes.

53 ional reconstitution of the heterodimer into proteoliposomes.

54 ted by MccJ25 in both detergent solution and proteoliposomes.

55 Proteoliposomes (50-150 nm in diameter) obtained from re

56 iffuse fashion, while films prepared from bR-proteoliposomes added to the diC(6) PC/yeast solution re

57 Delivery of syndecan-4 proteoliposomes also increased endothelial proliferation

58 bunit, Cdc50p, also was reconstituted in the proteoliposome, although at a substoichiometric concentr

59 r, when t-SNAREs were reconstituted into the proteoliposome and mixed with the soluble SNARE motif of

60 between synaptobrevin-2 (VAMP-2)-containing proteoliposomes and acceptor t-SNARE complex-containing

61 Using purified Pgp incorporated into proteoliposomes and ATP-gamma-35S, we demonstrate that t

62 Ab-phage library and a panning strategy with proteoliposomes and cells to identify seven human nAbs a

63 Assays using these robust reconstituted proteoliposomes and cytoplasmic membrane vesicles have r

64 reconstituted the TpsB transporter FhaC into proteoliposomes and demonstrate that FhaC is the sole ou

65 Both proteoliposomes and empty lipid vesicles lacking rhodops

66 explored BtuCD-F function in vitro, both in proteoliposomes and in various detergents.

67 f the homologous SERCA1a in co-reconstituted proteoliposomes and mimic the 2b-tail effect (i.e. lower

68 main motions in the context of reconstituted proteoliposomes and physiological ion gradients using si

69 ngle particle fusion between synaptobrevin-2 proteoliposomes and planar-supported bilayers containing

70 d-)SNAP-25 are separately reconstituted into proteoliposomes and subsequently assembled in the plane

71 scopy allowed us to count both the number of proteoliposomes and the number of protein-containing mic

72 ymatic digestion is performed on immobilized proteoliposomes and the resulting cleaved peptides are a

73 active synaptic proteins reconstituted into proteoliposomes and their interactions in a native membr

74 Purified protein was reconstituted into proteoliposomes and urea efflux was measured by stopped-

75 use of a modified vesicle fusion method from proteoliposomes and visualized with contact mode atomic

76 by stopped flow with LacY in detergent or in proteoliposomes and were compared with rates of galactos

77 ously to interfere with lipid mixing between proteoliposomes and with synaptic transmission, Syt1-R39

78 bR was first incorporated into liposomes (bR-proteoliposomes) and then added to an S. cerevisiae solu

79 ., Mg(2+) and SO(4)(2-)) on the stability of proteoliposomes, and design criteria for aquaporin-based

80 endent reduction was studied in solution, in proteoliposomes, and in Nanodiscs.

81 e architecture is formed by self-assembly of proteoliposomes, and its structure is characterized by s

82 nsport could be established in reconstituted proteoliposomes, and silicon uptake was found to be depe

83 ved either inside or on the outer surface of proteoliposomes, and strikingly, several thinner fibrils

84 (2) stimulated fusion when it was on R-SNARE proteoliposomes, apposed to Q-SNARE proteoliposomes bear

85 sporters in Arabidopsis The application of a proteoliposome-based transport assay revealed that four

86 Complementary proteoliposomes bearing a Rab:GTP and either the vacuola

87 With proteoliposomes bearing asymmetrically disposed SNAREs,

88 o report this arrangement of fibrils made by proteoliposomes bearing CesA8 from hybrid aspen.

89 R-SNARE proteoliposomes, apposed to Q-SNARE proteoliposomes bearing PI(3)P.

90 Although fused proteoliposomes became unstable at elevated SNARE concen

91 of rhodopsins previously observed in ASR in proteoliposomes being preserved.

92 itoring (QCM-D) which showed that 95% of the proteoliposomes bind within 5 min.

93 When reconstituted into proteoliposomes, both purified P44s exhibited porin acti

94 to stimulate SNARE-mediated lipid mixing of proteoliposomes by a factor of 5 at a physiological conc

95 t simple model membrane system, by preparing proteoliposomes by hydration of a mixed film of Abeta pe

96 condary active transporters reconstituted in proteoliposomes by measuring transporter equilibrium pot

97 GLIC function and dynamics in reconstituted proteoliposomes by patch clamp measurements and EPR spec

98 ents are replaced with lipids, the resulting proteoliposomes catalyze protein-mediated sugar transpor

99 e vacuolar proteins was required for optimal proteoliposome clustering, raising the question of which

100 ow cell was found to bind a maximum of 1 mug proteoliposomes/cm(2), and a minimum proteoliposome conc

101 For both crude membrane fractions and proteoliposomes composed of lens proteins in phosphatidy

102 lipid-order and bovine rhodopsin function in proteoliposomes composed of the sn-1 chain perdeuterated

103 dy systematically investigated the effect of proteoliposome composition (lipid type, protein-to-lipid

104 f 1 mug proteoliposomes/cm(2), and a minimum proteoliposome concentration required for saturation of

105 We prepared rhodopsin-containing proteoliposomes consisting of asolectin or native retina

106 nowire transistor devices are made by fusing proteoliposomes containing a bacteriorhodopsin (bR) prot

107 otic stress measurements were performed with proteoliposomes containing AQP4 and three different lipi

108 Here, we use proteoliposomes containing complex I, together with a qu

109 In proteoliposomes containing Drs2p, a phosphatidylserine a

110 act cells, inside-out membrane vesicles, and proteoliposomes containing functionally reconstituted pu

111 g transport with known amounts of protein in proteoliposomes containing hSMVT in outside-out orientat

112 -assembled system without these limitations: proteoliposomes containing mammalian complex I, Q10, and

113 )IV(1) were formed after reconstitution into proteoliposomes containing only phosphatidylcholine and

114 Using reconstituted proteoliposomes containing particular combinations of th

115 rt of Hoechst 33342 in membrane vesicles and proteoliposomes containing purified and functionally rec

116 the ER undergoing delayed fusion in vivo and proteoliposomes containing purified Sey1p fused in a GTP

117 901035j ) further probes efflux kinetics for proteoliposomes containing purified, reconstituted PfCRT

118 For pre-steady-state current measurements, proteoliposomes containing SERCA and phospholamban or sa

119 In this method, proteoliposomes containing Shaker potassium channels are

120 Proteoliposomes containing sphingolipids significantly i

121 Proteoliposomes containing the cleaved GPC mediate pH-de

122 his study we packaged the DNA of interest in proteoliposomes containing the fusogenic galactose-termi

123 Proteoliposomes containing the membrane-fusing GTPase Se

124 with purified S. cerevisiae proteins, using proteoliposomes containing the multi-spanning ubiquitin

125 ke activity is not detectable in P(i)-loaded proteoliposomes containing the p.R28H G6PT null mutant.

126 receptor concentration on kinase activity in proteoliposomes containing the purified Escherichia coli

127 The assays monitor fusion between proteoliposomes containing the synaptic vesicle SNARE sy

128 Fusogenic proteoliposomes, containing charged lipids and membrane

129 d by negative electric surface potentials of proteoliposomes controlled by the content of anionic CHS

130 We observed the rate of osmotic proteoliposome deflation by light scattering.

131 he range of 0.1-1 pN, too small to cause any proteoliposome deformation or rupture.

132 rdings of the peptide alamethicin and of the proteoliposome-delivered potassium channel KcsA demonstr

133 ing hits are multimerized by nanoparticle or proteoliposome display, and they are evaluated for BCR t

134 Here we report on two different proteoliposome-doped gels (proteogels) whose properties

135 f the flow cell, and the force acting on the proteoliposomes during flow cell operation was estimated

136 Past experiments with reconstituted proteoliposomes, employing assays that infer membrane fu

137 is a suitable tool for shotgun proteomics on proteoliposomes, enabling more detailed characterization

138 c assay based on the equilibrium exchange of proteoliposome-entrapped radioactive tracers.

139 Proteogels containing bR proteoliposomes exhibit a stable proton gradient when ir

140 Purified ZnT8 variants in proteoliposomes exhibited more than 4-fold functional tu

141 These proteoliposomes exhibited succinate counterflow activity

142 roviding a continuous flow of electrons, the proteoliposome experiments demonstrate that conduction t

143 ity, pf, yielded similar values for cell and proteoliposome experiments.

144 onment and necessitating reconstitution into proteoliposomes for activity measurements.

145 that a purified ENT can be reconstituted in proteoliposomes for functional characterization in a def

146 As a means of preparing proteoliposomes for the study of membrane proteins, seve

147 We report conditions that allow pH-dependent proteoliposome formation and identify a sequence at the

148 clude a detergent-removal step to facilitate proteoliposome formation, and this increased baseline ac

149 Furthermore, the proteoliposome formulation may provide a model for vacci

150 bioorthogonal coupling reactions to generate proteoliposomes from micelle-solubilized proteins.

151 On the other hand, the results from the proteoliposome fusion assay, employing cysteine- and nit

152 We addressed this puzzle in reconstituted proteoliposome fusion reactions with all-purified compon

153 Using a reconstituted proteoliposome fusion system, we show that trans-SNARE c

154 -dependent GLUT4 vesicle fusion in a defined proteoliposome fusion system.

155 Pase Ypt7 is essential on both membranes for proteoliposome fusion to occur at these SNARE concentrat

156 y thinned around the aperture, and unimpeded proteoliposome fusion, enabled by the large aperture dia

157 OPS, for optimal membrane tethering, and for proteoliposome fusion.

158 ittle effect on the rates of synaptobrevin-2 proteoliposome fusion.

159 Proteolipobeads formed from HeLa proteoliposomes gave rise to homogeneous distributions o

160 ort the reconstitution of M5-DLO flipping in proteoliposomes generated from Triton X-100-extracted Sa

161 Using reconstituted proteoliposomes harboring partially purified recombinant

162 BtuCD reconstituted into proteoliposomes has a significant basal ATP hydrolysis r

163 These robust proteoliposomes have evolved naturally to be resistant t

164 for full association of HOPS and Sec18p with proteoliposomes having a minimal set of lipids.

165 The rate of proteoliposome immobilization was determined using a qua

166 or by fusion of purified native vesicles or proteoliposomes in preformed GUVs.

167 g the coincident fluorescence of solutes and proteoliposomes in the focal volume of a confocal micros

168 efficient fusion of the reconstituted SNARE proteoliposomes in the presence of SNARE chaperones.

169 titution system for the translocon SecYEG in proteoliposomes in which 55% of the accessible transloco

170 ug extrusion by NorM from Vibrio cholerae in proteoliposomes in which purified NorM protein was funct

171 onstrated by a fluorescence-based assay with proteoliposomes in which the Mrp complex was coreconstit

172 re recorded upon addition of Na(+) to PaNhaP proteoliposomes, indicating a reaction where positive ch

173 rity of animals immunized with gp41(int)-Cys proteoliposomes induced modest breadth and potency in ne

174 thout the Ca(2+) sensor synaptotagmin-1) and proteoliposomes initially containing the plasma membrane

175 mensional structures of membrane proteins in proteoliposomes is demonstrated by determining the struc

176 r dehydration followed by rehydration of the proteoliposomes is essential.

177 osmolality when the medium outside cells or proteoliposomes is supplemented with diverse, membrane-i

178 Man-P-Dol(10) transport into reconstituted proteoliposomes is time-dependent, reversible, saturable

179 sion properties of chemically defined SNARE- proteoliposomes, it has been proposed that the assembly

180 ccur rapidly following a threshold change in proteoliposome lipid composition.

181 flipping of lipids triggered by a change in proteoliposome lipid composition.

182 f Escherichia coli (LacY) reconstituted into proteoliposomes loaded with a pH-sensitive fluorophore e

183 was more anion-dependent than Pi(1/2)/RT for proteoliposomes loaded with citrate, sulfate, phosphate,

184 This was attributed to acidification of the proteoliposome lumen due to H(+)-proline symport.

185 tant for osmosensing in E. coli cells and in proteoliposomes made from E. coli phospholipids.

186 n as both an osmosensor and osmoregulator in proteoliposomes made from Escherichia coli phospholipids

187 an animal model of limb ischemia, syndecan-4 proteoliposomes markedly improved the neovascularization

188 geneities can severely skew ensemble-average proteoliposome measurements but also enable ultraminiatu

189 The G6PT-proteoliposome-mediated G6P or P(i) uptake is inhibited

190 ificantly enhance drug permeation across the proteoliposome membranes.

191 nt, i.e. in intact C. glutamicum cells or in proteoliposomes mimicking the composition of the C. glut

192 membrane is invariably more complex than the proteoliposomes most often used for solid-state NMR (SSN

193 urified yeast Yop1p and incorporated it into proteoliposomes, narrow tubules (approximately 15 to 17

194 ssential fusion components, we reconstituted proteoliposomes of a more physiological composition, bea

195 -terminal tail of SLN using co-reconstituted proteoliposomes of SERCA and SLN.

196 s showed an even distribution of immobilized proteoliposomes on the surface.

197 table as a potent surrogate for conventional proteoliposomes or immunoliposomes as a result of excell

198 in which the ion channel TRPV1, contained in proteoliposomes or in excised patches, was exposed to li

199 affect either the physical parameters of the proteoliposomes or the ability of SNAREs to drive lipid

200 bundles that either covered the surfaces of proteoliposomes or were spawned from liposome surfaces.

201 t optimized" pfcrt genes, reconstituted into proteoliposomes (PL), and efflux of NBD-CQ was measured

202 The purified Se-HAS is reconstituted into proteoliposomes (PLs) where it synthesizes and transloca

203 tin) and Cy5-streptavidin trapped in the two proteoliposome populations) are measured simultaneously

204 either increasing or decreasing PE levels in proteoliposomes postreconstitution of LacY, clearly demo

205 Ci-VSD reconstitutes essentially randomly in proteoliposomes, precluding straightforward application

206 Proteoliposomes prepared at a protein/phospholipid/CHS m

207 Under identical conditions, proteoliposomes prepared by RD have a higher L/P ratio,

208 t of MII formation is up to 3-fold faster in proteoliposomes prepared by RD vs DD.

209 However, the larger, more homogenous proteoliposomes prepared by the direct method yield almo

210 erence in rhodopsin activity was observed in proteoliposomes prepared by these techniques.

211 er, the functionality of membrane protein in proteoliposomes prepared by various techniques has rarel

212 ty can be reconstituted in large unilamellar proteoliposomes prepared from phosphatidylcholine and Tr

213 PI(3)P on Q-SNARE proteoliposomes promoted Vam7p binding and association w

214 When reconstituted into proteoliposomes, purified P51, but not Nsp3, exhibited p

215 tly demonstrated M5-DLO flipping activity in proteoliposomes reconstituted from detergent-solubilized

216 Using proteoliposomes reconstituted from Triton X-100-solubili

217 Proteoliposomes reconstituted here from purified membran

218 by Drs2p, because protein-free liposomes or proteoliposomes reconstituted with a catalytically inact

219 Experiments in proteoliposomes reconstituted with alpha1beta1 show anal

220 fractionation, and analyses of flip-flop in proteoliposomes reconstituted with ER membrane proteins

221 Docking and fusion of single proteoliposomes reconstituted with full-length v-SNAREs

222 Proteoliposomes reconstituted with human purified Band 3

223 The porin activity of proteoliposomes reconstituted with proteins from the out

224 In proteoliposomes reconstituted with purified, N-terminall

225 , and enhances hemifusion and full fusion of proteoliposomes reconstituted with the target (t)-SNAREs

226 Functional proteoliposomes, reconstituted from a microsomal deterge

227 Proteoliposome reconstitution is a standard method to st

228 Further addition of cardiolipin to the proteoliposome reconstitution mixture resulted in distin

229 in detergent micelles or reconstituted into proteoliposomes reveals dramatic increases in galactosid

230 Reconstituted into proteoliposomes, RhCG conducts NH(3) to raise internal p

231 By using this protocol, proteoliposome samples for magic-angle spinning NMR and

232 the time course of fusion events between two proteoliposomes should be useful for addressing many imp

233 Real-time ATP synthesis experiments in proteoliposomes show the mutant enzyme, harboring the la

234 Antibody-binding assay and proteolysis of proteoliposomes showed physical evidence of preferential

235 On the cytoplasmic side of LacY in proteoliposomes, slow bimane quenching (i.e., closing of

236 NtPDR1 reconstitution in proteoliposomes stimulated its basal ATPase activity fro

237 Proteoliposome studies have suggested that SNAREs drive

238 In the present system, proteoliposome studies of purified FUN26 demonstrate rob

239 electrodiffusion flux assays on endomembrane proteoliposomes suggested that *O(2)(-) and Cl(-) are tr

240 to stimulate G6P uptake in P(i)-loaded G6PT-proteoliposomes, suggesting that the G6Pase-alpha-mediat

241 mol-1 for the transporter reconstituted into proteoliposomes, suggesting that the substrate-loaded tr

242 f wild-type and mutant proteins in cells and proteoliposomes support our proposal for the mechanistic

243 freeze-fracture electron microscopy of GLUT1 proteoliposomes support the hypothesis that the glucose

244 ennetsu had porin activity, as measured by a proteoliposome swelling assay.

245 es have porin activities, as determined by a proteoliposome swelling assay.

246 A proteoliposome system containing a pool of internalized

247 ological organization of LacY in an in vitro proteoliposome system in which lipid composition can be

248 Using a proteoliposome system with purified SRI in complex with

249 Outer membrane vesicles (OMVs) are nanoscale proteoliposomes that are ubiquitously secreted by Gram-n

250 Using SNARE-decorated proteoliposomes that cannot fuse on their own, we now de

251 Upon reconstitution into proteoliposomes, the ATPase activity of MacB was strictl

252 reconstitution of the Gly-->Trp mutants into proteoliposomes, the concentration dependence of sugar-b

253 With LacY in proteoliposomes, the periplasmic cavity is tightly seale

254 ehensive functional studies on reconstituted proteoliposomes, the structures explain the transport me

255 When reconstituted in proteoliposomes, these proteins avidly fuse with liposom

256 However, sedimentation of unilamellar proteoliposomes through an iso-osmotic gradient showed t

257 al proteolytic protocol, repeatedly exposing proteoliposomes to a digestive enzyme, trypsin, was deve

258 with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of mem

259 rane proteins are typically reconstituted in proteoliposomes to observe their function in a physiolog

260 ognized by the PX domain of Vam7p on Q-SNARE proteoliposomes to promote tethering, although this func

261 6(+/+); administration of purified RLIP76 in proteoliposomes to RLIP76(+/+) animals further increased

262 Increasing the PLR of DOPC proteoliposomes up to 1:200 increased their osmotic wate

263 ntrareconstitution heterogeneities in single proteoliposomes using fluorescence microscopy.

264 iminished the transient currents measured in proteoliposomes using solid supported membrane-based ele

265 econstituted beta-barrel protein assembly in proteoliposomes using the enzymatic activity of a protei

266 incorporated in planar lipid bilayers and in proteoliposomes, using a novel flux-based assay.

267 trial with a novel autologous, tumor-derived proteoliposome vaccine formulation that could be rapidly

268 detect directly because of the leakiness of proteoliposomes, vesicle aggregation and rupture that of

269 When the interior of the proteoliposomes was acidified, the addition of aminoglyc

270 Importantly, bR introduced via proteoliposomes was observed to modulate pH gradients de

271 PI(3)P on R-SNARE proteoliposomes was recognized by the PX domain of Vam7p

272 Tethering of the SNARE-bearing proteoliposomes was required for efficient lumenal compa

273 antification of the per-channel increment in proteoliposome water permeability with the aid of stoppe

274 Using reconstituted proteoliposomes we show that both G6P and P(i) are effic

275 I) and the xanthophyll zeaxanthin (Zea) into proteoliposomes, we have tested the individual contribut

276 using purified components reconstituted into proteoliposomes, we show that Msp1 is both necessary and

277 These SecYEG proteoliposomes were active in translocation assays.

278 The gp41(int)-Cys proteoliposomes were administered alone or in prime-boos

279 Electrochemical analyses of proteoliposomes were compared with analyses of both prot

280 ere formed in the DD preparation, while only proteoliposomes were formed in the RD preparation.

281 oyl-sn-glycero-3-phosphocholine (DOPC)-based proteoliposomes were found to have excellent osmotic wat

282 a K(+)-containing medium to which K(+)-free proteoliposomes were fused.

283 When these proteoliposomes were incubated with those bearing cognat

284 In the current study, proteoliposomes were prepared using AquaporinZ from Esch

285 gnificant recovery of function occurred when proteoliposomes were treated with MTSEA (methanethiosulf

286 ensitive factor attachment protein receptor) proteoliposomes when they have Ypt7p bearing either GDP

287 protein was purified and reconstituted into proteoliposomes, where sodium dependence of sugar transp

288 nching was not active in reconstituted LHCII proteoliposomes, whereas a high degree of energetic conn

289 ably due to the occurrence of defects in the proteoliposomes, whereas the addition of cholesterol imp

290 emonstrated measuring uptake of [(3)H]Arg in proteoliposomes which was trans-stimulated by internal A

291 y incorporation into Nanodiscs or into large proteoliposomes with a high lipid-to-protein (L/P) ratio

292 , and at high resolution to yield functional proteoliposomes with a narrow size distribution for furt

293 ositive charge is rapidly displaced into the proteoliposomes with a rate constant of k >200 s(-1) We

294 nto liposomes and, after energization of the proteoliposomes with acid-base transitions, the initial

295 visible light, whereas proteogels containing proteoliposomes with both bR and F(0)F(1)-ATP synthase c

296 rmed 100 nm liposomes (direct method) yields proteoliposomes with more homogeneous sizes and protein

297 ty of lipid-anchored Nyv1p, we reconstituted proteoliposomes with purified lipid-anchored Nyv1p as th

298 cause both fusion and lysis, fusion of these proteoliposomes with Sec17p/Sec18p and HOPS is not accom

299 ent after fusion had occurred, reconstituted proteoliposomes with substantially reduced SNARE concent

300 ed ATPase activity in native membranes or in proteoliposomes, with IC50 values in the 10-40 nm range.