ライフサイエンスコーパス: protoplast

1 vitro (in nuclear extracts) and in vivo (in protoplasts).

2 the inner membrane that surrounds the spore protoplast.

3 c acid probe sequences can be uptaken by the protoplasts.

4 GFP transport to the plastids of Arabidopsis protoplasts.

5 across the plasma membrane of root epidermal protoplasts.

6 rs to the tonoplast in Arabidopsis mesophyll protoplasts.

7 phisticated thylakoid architecture in intact protoplasts.

8 es in response to ABA in B. napus guard cell protoplasts.

9 by the CryR1 cis-element in Arabidopsis leaf protoplasts.

10 tid envelope after expression in Arabidopsis protoplasts.

11 nidase, repressed its translation in tobacco protoplasts.

12 regulator exclusively in the nucleus of rice protoplasts.

13 XXT1, XXT2, and XXT5 proteins in Arabidopsis protoplasts.

14 the SARD1 promoter in yeast and Arabidopsis protoplasts.

15 e plasma membrane of tobacco Bright Yellow-2 protoplasts.

16 human cells and ADH1 in Arabidopsis thaliana protoplasts.

17 imilar to those produced in p1-5b-inoculated protoplasts.

18 support viral genome replication in tobacco protoplasts.

19 Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts.

20 aling by transient expression in Arabidopsis protoplasts.

21 a gusA gene into tobacco (Nicotiana tabacum) protoplasts.

22 of RNAi knockout genes would be feasible in protoplasts.

23 lso be utilized to examine gene functions in protoplasts.

24 a and H4b region can mediate satC fitness in protoplasts.

25 Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts.

26 iently transformed maize Black Mexican Sweet protoplasts.

27 protein-protein interactions in Arabidopsis protoplasts.

28 taxin of plant) 51 and SYP61, in Arabidopsis protoplasts.

29 transient expression analysis in Arabidopsis protoplasts.

30 K MPK4 in vitro, and activates it in vivo in protoplasts.

31 t yeast strains or electroporated into plant protoplasts.

32 n transient expression assays in Arabidopsis protoplasts.

33 says and functional studies in nontransgenic protoplasts.

34 nt system was adapted to function in tobacco protoplasts.

35 toplasts than in pRTL2-GFP:TGBp2-transfected protoplasts.

36 ent protein (GFP) chimera in vivo in tobacco protoplasts.

37 cation enhancement activity of C3 in tobacco protoplasts.

38 were monitored in Arabidopsis leaf mesophyll protoplasts.

39 shed BMV RNA synthesis in transfected barley protoplasts.

40 cell and population levels using Arabidopsis protoplasts.

41 modest effects on BMV replication in barley protoplasts.

42 ere found to be important for RNA4 levels in protoplasts.

43 Lilium longiflorum pollen grain and tube tip protoplasts.

44 ssion analysis, in Black Mexican Sweet maize protoplasts.

45 as a very strong silencing suppressor in the protoplasts.

46 and degradation of ricin A chain in tobacco protoplasts.

47 protein(s) that caused plasmolysis of plant protoplasts.

48 ach for the tracking of rare RNAs within the protoplasts.

49 Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts.

50 for the accumulation of full-length PEMV in protoplasts.

51 d with individual amiRNA candidates in plant protoplasts.

52 neration of plants from Arabidopsis thaliana protoplasts.

53 pulus trichocarpa stem differentiating xylem protoplasts.

54 ivate hydrolytic enzyme promoters in soybean protoplasts.

55 fluorescence complementation in Arabidopsis protoplasts.

56 electron microscopy, and cell separation by protoplasting.

57 At pH 5.7, the majority of auxin influx into protoplasts - 75% - was mediated by the influx carrier A

58 In contrast, in mature epidermal protoplasts a plasma membrane hyperpolarization-activate

59 Transient expression in Arabidopsis thaliana protoplasts also reveals that TgMTP1::green fluorescent

60 Protoplast and oocyte swelling assays showed that PIP2;5

61 Protoplast and test-tube reconstitution assays were used

62 smotic water permeability (Pf) of guard cell protoplasts and an accumulation of reactive oxygen speci

63 microscopy in Arabidopsis thaliana mesophyll protoplasts and bimolecular fluorescence complementation

64 ic plant parts using transient expression in protoplasts and dual luciferase outputs.

65 e in two transient expression systems: wheat protoplasts and epidermal cells of mature embryos.

66 Arsenic efflux was measured from protoplasts and from intact plants, and arsenic levels w

67 nd EVR at the plasma membrane of Arabidopsis protoplasts and hypothesize that CST negatively regulate

68 a TF gene affecting wood formation, in these protoplasts and identified differentially expressed gene

69 ment inhibits TBSV RNA accumulation in plant protoplasts and in Nicotiana benthamiana leaves.

70 inimal promoter in tobacco, corn and soybean protoplasts and in transgenic Arabidopsis and tobacco pl

71 In protoplasts and plants inoculated with PVX-GFP:TGBp2 or

72 Arabidopsis (Arabidopsis thaliana) protoplasts and plants were transiently and stably trans

73 protein localized to the plasma membrane in protoplasts and plasmolysis experiments, suggesting that

74 ive gene expression was induced in wild-type protoplasts and restored in nph4-1 protoplasts only with

75 Splicing analyses of constructs in protoplasts and RNA from overexpression lines used high-

76 fully transactivates the VPE gene in soybean protoplasts and that this transactivation was associated

77 can be achieved in ~60 s, with isolation of protoplasts and their subsequent transfection taking ~50

78 na benthamiana leaves, Arabidopsis mesophyll protoplasts and tobacco BY-2 protoplasts, regardless of

79 ctivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds directly to

80 ng three different clusters of genes in rice protoplasts and verification of deletions of two cluster

81 YB305 fusion localized to nucleus of tobacco protoplasts and yeast one-hybrid assays demonstrated tha

82 h it is not irreversibly associated with the protoplast, and presumably resides outside the cell memb

83 onto polysomes and stimulates translation in protoplasts, and both processes are sensitive to TOR inh

84 vated the promoter of AAO3 in mesophyll cell protoplasts, and electrophoretic mobility shift assays s

85 uses cGMP-dependent net water uptake into WT protoplasts, and hence volume increases, whereas respons

86 AvrPtoB(1-307) was phosphorylated in tomato protoplasts, and mass spectrometry identified serine 258

87 synthetic parts in sorghum (Sorghum bicolor) protoplasts, and the results showed that our method work

88 encoding AL1 were cotransfected into tobacco protoplasts, and viral DNA replication was monitored by

89 c water permeability of isolated root cortex protoplasts, aquaporin gene expression, aquaporin abunda

90 pts are overexpressed in cls leaves, and cls protoplasts are more sensitive to programmed cell death

91 Because protoplasts are nongrowing cells, effective RNAi-trigger

92 Wheat root protoplasts are patch clamped in the whole-cell configur

93 the preparation of bioactive COS and fungal protoplasts, as biocontrol agent against pathogenic fung

94 Both are expressed strongly in guard cell protoplasts, as determined by reverse transcription-poly

95 We use membrane photobleaching and protoplast assays to demonstrate that SpoIIIE is require

96 ility shift, yeast one-hybrid, and C. roseus protoplast assays.

97 ere localized to a small region of the grain protoplasts associated with the site of tube germination

98 ions across the plasma membrane of a single protoplast at multiple voltages and in complex physiolog

99 NAs still allowed RNA accumulation in barley protoplasts at significant levels.

100 In mesophyll protoplasts, AtECA3-green fluorescent protein associated

101 Our analyses suggest that protoplast-based transient RNA silencing is a useful exp

102 Yeast- or protoplast-based two-hybrid and bimolecular fluorescent

103 In an Arabidopsis-protoplast beta-glucuronidase reporter gene assay, as we

104 ssing GFP fused to TGBp3 were transfected to protoplasts, bombarded to tobacco leaves, and studied in

105 imes less efficiently than wild-type TGMV in protoplasts but produced severe symptoms that were delay

106 t can accumulate to wild-type (wt) levels in protoplasts but remain less fit in planta when competed

107 a methodology for the isolation of idioblast protoplasts by fluorescence-activated cell sorting is es

108 rod-shaped cells are converted into spheres (protoplasts) by treatment with lysozyme, DivIVA adopts a

109 e were transfected into three distinct plant protoplast cell systems.

110 Rice protoplast cells transformed with Cas9/sgRNA constructs

111 dergoes frequent recombination in plants and protoplast cells when it carries the AU-rich hot spot se

112 Therefore, RNAi in protoplasts complements existing genetic tools, as it al

113 Experiments with protoplasts confirmed that the coexpression of MYB77 and

114 Furthermore, a protoplast cotransfection assay showed that BBX24 and BB

115 C1 and LEC2 proteins produced in Arabidopsis protoplasts could form a ternary complex with NF-YC2 in

116 eins, when expressed in qed1 or rare1 mutant protoplasts, could not complement the editing defect.

117 In plant protoplasts, cryptochrome activation results in rapid in

118 PEG-calcium transfection of plasmid DNA and protoplast culture.

119 histone variant family, were observed during protoplast culture.

120 To investigate this, leaf protoplast cultures were cofed with 13C6-labeled conifer

121 e-cell patch-clamp measurements on root cell protoplasts demonstrated that Ag NPs slightly inhibited

122 quired for MS channel activities detected in protoplasts derived from root cells.

123 Vacuoles were isolated from protoplasts derived from rosette leaf tissue.

124 me-wide transcript profiles of plantlets and protoplast-derived cells (PdCs) during the first week of

125 ORMATION4, is required for the initiation of protoplast division.

126 Furthermore, PSKR1-deficient protoplasts do not expand in response to PSK but are sti

127 momycin binding to intact sucrose-stabilized protoplasts, even though the drug bound normally to the

128 ting to isolate responses in the transformed protoplasts exclusively.

129 e unresponsive to PSK, and bak1-3 and bak1-4 protoplasts expanded less in response to PSK but were fu

130 PSK promotes protoplast expansion in the wild type but not in cngc17.

131 Protoplast expansion is likewise promoted by cGMP in a C

132 Protoplasting experiments indicated that both GGT1 and G

133 aize Ubiquitin promoter for use in transient protoplast expression assays and particle bombardment tr

134 responsive genes in a Daucus carota (carrot) protoplast expression system.

135 Expression of the splicing reporter in protoplasts faithfully produced all splice variants from

136 l disruption of mesophyl-derived Arabidopsis protoplasts, followed by a density gradient fractionatio

137 Protoplast formation and transformation takes less than

138 We isolated a total of 3 x 10(8) guard cell protoplasts from 22,000 Arabidopsis thaliana plants and

139 tilized approximately 350 million guard cell protoplasts from approximately 30,000 plants of the Arab

140 Protoplasts from BGL-1 lines divide and form calli witho

141 y in insect cell cultures and in Arabidopsis protoplasts from cryptochrome mutant seedlings.

142 gnition site for a zinc-finger nuclease, and protoplasts from each tobacco line were electroporated w

143 s, either by overexpression or by the use of protoplasts from the corresponding mutants.

144 e volume increases, whereas responses of the protoplasts from the receptor mutant are impaired.

145 n Kros and the osmotic water permeability of protoplasts from the veins but not from the mesophyll.

146 Finally, because we isolated protoplasts from tissues of 14-d-old seedlings instead o

147 late 50% less [(3)H]MTX than the vacuoles of protoplasts from wild-type plants when incubated in medi

148 otocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-different

149 Cultured guard cell protoplasts (GCP) of tree tobacco (Nicotiana glauca) com

150 Cultured tree tobacco guard cell protoplasts (GCPs) are useful for studying the effects o

151 ll surface area to volume ratio in wild-type protoplasts generates similar shape changes and cell div

152 xpression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool f

153 etion of a targeted polypeptide and, because protoplasts have a limited life span, if functional assa

154 In transiently transfected Arabidopsis protoplasts, HYL1-mCherry and YFP-DCL1 fusion proteins c

155 tobacco (Nicotiana tabacum) Bright Yellow-2 protoplasts identified single cDNA clones encoding prote

156 ed SIZ1-mediated sumoylation in vitro and in protoplasts identifying the K393 residue as the principa

157 A did not accumulate to detectable levels in protoplasts in the absence of the kl-TSS.

158 the cellular level using vitality stains in protoplasts, in intact seedlings grown on agar plates an

159 te ring mechanisms, we studied fission yeast protoplasts, in which constriction occurs without the ce

160 ar fractionation of pea (Pisum sativum) leaf protoplasts indicated that 30% of lysophosphatidylcholin

161 XXT2YFP and XXT5HA proteins from Arabidopsis protoplasts indicated that while the formation of the XX

162 scription factor in vitro and in Arabidopsis protoplasts, indicating that ABI5 is sumoylated through

163 Overexpression of DIG3 in tobacco BY-2 protoplasts inhibited nuclear import of a beta-glucuroni

164 After cleavage of the protoplast into aplanospores, a vacuole and lipids remai

165 to the host cells, the parasite injected its protoplast into the host between the host cell wall and

166 Transient genetic modification of plant protoplasts is a straightforward and rapid technique for

167 Cell division in protoplasts is enhanced 7-fold in the presence of exogen

168 Radiolabeled auxin uptake was measured in protoplasts isolated from roots of Arabidopsis thaliana.

169 The vacuoles of protoplasts isolated from the leaves of Wassilewskia atm

170 Protoplasts, isolated from filamentous gametophytic tiss

171 es of control experiments, establishing that protoplast isolation and cell sorting procedures did not

172 The protoplast isolation and DNA transfection procedures tak

173 ue preparation, digestion of SDX cell walls, protoplast isolation and DNA transfection.

174 The core procedure, from protoplast isolation to identification of optimal amiRNA

175 The method includes protoplast isolation, PEG-calcium transfection of plasmi

176 The whole procedure, including protoplast isolation, takes approximately 6 h.

177 behavior when expressed in tobacco mesophyll protoplasts: KAT2 forms homotetrameric channels active a

178 In addition to protoplasts, leaf epidermal cells transiently transforme

179 In protoplasts, LhG4AtO achieved maximum transactivation of

180 morphology of avenaciolide-treated cells was protoplast-like, which indicated that cell wall biosynth

181 r the pectin activation of numerous genes in protoplasts, many of which are involved in cell wall bio

182 rinated analogue of oritavancin, to isolated protoplast membranes and whole-cell sucrose-stabilized p

183 In isolated protoplast membranes, both with and without 1 M sucrose

184 show that overexpressed Ggamma1 localizes to protoplast membranes, but Gbeta exhibits membrane locali

185 ssociated vacuoles are detected in mesophyll protoplasts of des1 mutants.

186 ave developed an RNA silencing system in the protoplasts of Nicotiana benthamiana to investigate the

187 Analysis of GspB in protoplasts of secA2 or secY2 mutant strains, which do n

188 membranes and whole-cell sucrose-stabilized protoplasts of Staphylococcus aureus, grown in media con

189 ry) was aberrant in roots and root epidermal protoplasts of the Atann1 knockout mutant.

190 IA PLA(2) activity toward cell wall-depleted protoplasts of the wt and tagO strains of S. aureus or i

191 GFP-intron-GFP reporter was investigated in protoplasts of three single and three double mutants of

192 Our functional results in Zea mays mesophyll protoplasts on ABA-inducible expression effects on the L

193 wild-type protoplasts and restored in nph4-1 protoplasts only with auxin and not with other hormones,

194 ative SYP121-Sp2 fragment in maize mesophyll protoplasts or epidermal cells leads to a decrease in th

195 ed either volume changes of single hypocotyl protoplasts or hypocotyl growth, both at high temporal r

196 ed TBSV replication in Nicotiana benthamiana protoplasts or in whole plants.

197 Transient gene expression, in plant protoplasts or specific plant tissues, is a key techniqu

198 tabolites in Arabidopsis thaliana guard cell protoplasts over a time course of ABA treatment.

199 Protoplasts overexpressing both AtHD2B and RPS6 exhibite

200 In protoplasts pectins activate, in a WAK2-dependent fashio

201 ur protocol gives a high yield (~2.5 x 10(7) protoplasts per g of SDX) of protoplasts sharing 96% tra

202 n germination medium for at least 1 h before protoplast preparation.

203 enhance virus accumulation in the inoculated protoplasts, promote cell-to-cell virus movement in the

204 Metabolite profiling of the cofed protoplasts provided strong support for the occurrence o

205 occurred in more than 10% of the transformed protoplasts regardless of the reporter's chromosomal pos

206 opsis mesophyll protoplasts and tobacco BY-2 protoplasts, regardless of whether VirE2 was co-expresse

207 In plant protoplasts replicating all three BMV genomic RNAs, muta

208 c mutation did not alter viral DNA levels in protoplast replication assays.

209 on of HSP70h and the p61 protein with CPm in protoplasts restricted encapsidation to the 5' approxima

210 a membrane face of elongation zone epidermal protoplasts resulted in the appearance of a hyperpolariz

211 oduction of these four components into plant protoplasts results in ABA-responsive gene expression.

212 A transcriptional activation assay of protoplasts revealed that ABA treatment and coexpression

213 Reporter-fusion analyses in protoplasts revealed that, with a free N terminus, PGD1

214 a indicate that APY expression in guard cell protoplasts rises quickly when these cells are moved fro

215 culum (ER) in stably transformed Arabidopsis protoplasts, roots and root hairs.

216 Isolated protoplasts serve as a transient expression system that

217 d (~2.5 x 10(7) protoplasts per g of SDX) of protoplasts sharing 96% transcriptome identity with inta

218 In addition, arpc1 protoplasts show an increased sensitivity to osmotic sho

219 Transient co-transfections in Arabidopsis protoplasts showed that A-ZIP53 inhibited three bZIPs an

220 nt expression of AtCaM15 in Arabidopsis leaf protoplasts showed that AtCaM15 is present in the centra

221 ity assays performed in Arabidopsis and rice protoplasts showed that OsPCF2 and OsNIN-like4 are activ

222 Patch clamp recordings on moss protoplasts showed the presence of three distinct thermo

223 ty by transient expression in Physcomitrella protoplasts shows the PpLEA-1 promoter to be highly acti

224 by spongy mesophyll anatomy, decreasing with protoplast size and increasing with airspace fraction an

225 ast two-hybrid analysis and Arabidopsis leaf protoplast split luciferase assay, to demonstrate that m

226 Furthermore, expression of FLDK3R in fld protoplasts strongly reduced FLC transcription compared

227 previous results carried out in transfected protoplasts suggest that the hormone auxin can be bypass

228 al auxin response genes assayed in mesophyll protoplasts, suggesting that ARF7 plays a major role in

229 in the K+ flux-to-current ratio among single protoplasts suggests a heterogeneous distribution of KOR

230 ther cell engulfs the forespore to produce a protoplast, surrounded by two bilayer membranes, which s

231 stem overcomes the drawback that transfected protoplast suspensions are often a heterogeneous mix of

232 elated mutants and Coimbra, no auxin-induced protoplast swelling occurred.

233 be involved in mediating rapid auxin-induced protoplast swelling, but it is not involved in the contr

234 ated the TIR1/AFB pathway but did not induce protoplast swelling; instead, it showed auxin activity i

235 Using a parsley (Petroselinum crispum) protoplast system and a modified reporter gene vector wi

236 Furthermore, we developed a cotton protoplast system for transient gene expression to study

237 s investigated using an Arabidopsis thaliana protoplast system.

238 itor-responsive gene expression in a parsley protoplast system.

239 P fluorescence was greater in virus-infected protoplasts than in pRTL2-GFP:TGBp2-transfected protopla

240 teraction by luminescence within Arabidopsis protoplasts that express recombinant proteins at physiol

241 y direct measurement of iron levels in shoot protoplasts that intracellular iron levels in frd3 are o

242 t expression system in Arabidopsis mesophyll protoplasts that is highly amenable for the dissection o

243 We find that ACD2 shields root protoplasts that lack chlorophyll from light- and PPIX-i

244 enome edits when ssODNs were introduced into protoplasts that were pretreated with the glycopeptide a

245 hen expressed in tobacco (Nicotiana tabacum) protoplasts, the A chain of the heterodimeric toxin rici

246 ng peptide was fused to GFP and expressed in protoplasts, the fusion protein appeared only in chlorop

247 planta as in the moss Physcomitrella patens protoplasts, the presence of RY-like (RYL) elements is n

248 sider the case for local equilibrium between protoplasts, their cell walls, and adjacent air spaces d

249 In assays with transfected protoplasts, this repression was previously shown to occ

250 anslation in both wheat germ extract and oat protoplasts through a novel, noncanonical translation me

251 We used transfected barley protoplasts to examine the recognition of the subgenomic

252 ethod for the transformation of N. uniformis protoplasts to inactivate both nocK and nocL was develop

253 A protocol was developed with isolated protoplasts to obtain peripheral chloroplasts (P-CP), a

254 e use a luciferase reporter system in cowpea protoplasts to show that the 5' 217 nucleotides from TYM

255 This failure of arpc1 protoplasts to undergo proper tip growth is rescued by A

256 rmine binding sites in target promoters, and protoplast transactivation assays to demonstrate domains

257 Protoplasts transfected with PVX-GFP:TGBp2 or pRTL2-GFP:

258 or otherwise was detected in barley aleurone protoplasts transfected with the PpLEA-1::GUS construct.

259 c plus-strand, and subgenomic RNAs in barley protoplasts transfected with wild-type and mutant BMV tr

260 Arabidopsis protoplast transfection assays suggested that PtrMYB152

261 ion of primary/early auxin response genes in protoplast transfection assays.

262 We showed that protoplasts transfection with an in vitro-synthesized ds

263 A protoplast transformation method induced silencing more

264 Confocal microscopy of protoplasts transformed with enhanced green fluorescence

265 by quantitative real-time PCR (qRT-PCR) and protoplast transient assay.

266 Using a plant protoplast transient expression analysis we have further

267 ctionally important because Glc signaling in protoplast transient expression assays is compromised by

268 AtPTB1 and AtPTB2 was analysed in an in vivo protoplast transient expression system with a novel mini

269 oplasts, but also localized to nuclei during protoplast transient expression.

270 Protoplasts transiently cotransfected with promoter-luci

271 using fluorescence microscopy of A. thaliana protoplasts transiently expressing the N-terminal fusion

272 erexpression and is phenocopied in wild-type protoplasts treated with Latrunculin B, a potent inhibit

273 ession assays in tobacco (Nicotiana tabacum) protoplasts, TSAR1 and TSAR2 exhibit different patterns

274 -activated channel was detected in the grain protoplasts unless the grains were left in germination m

275 expression assays in Arabidopsis and lettuce protoplasts using a flagellin-based peptide.

276 ing assays in Arabidopsis thaliana mesophyll protoplasts using green fluorescent protein fusions, and

277 Sucrose solution visibly preserved the protoplast viability and slightly influenced the water d

278 he ABA activation of PLDalpha1 in leaves and protoplasts was attenuated in the SPHK mutants, and the

279 1)P, the nascent peptidoglycan of the intact protoplasts was confined to the membrane surface.

280 protein-tagged CHX20 expressed in mesophyll protoplasts was localized mainly to membranes of the end

281 SIZ1-dependent sumoylation of ICE1 in protoplasts was moderately induced by cold.

282 , translation in tobacco (Nicotiana tabacum) protoplasts was repressed by those constructs containing

283 thaliana leaf guard cell and mesophyll cell protoplasts was studied using the patch clamp technique.

284 VAMP722 was significantly increased when the protoplasts were incubated in the light.

285 The viable protoplasts were isolated from green cotyledons, etiolat

286 s restored in an auxin-dependent manner when protoplasts were transfected with a 35S:ARF7 effector ge

287 Protoplasts were transformed with BA-mgfp5-ER, in which

288 Root cell protoplasts were used to demonstrate that signalling to

289 to the cytoplasm of tobacco and Arabidopsis protoplasts, whether in the absence or presence of VIP1

290 in instability is enhanced in virus-infected protoplasts, which may account for the cytosolic and nuc

291 constitutively enhanced the Pf of guard cell protoplasts while suppressing its ABA-dependent activati

292 DNA accumulation by approximately 8-fold in protoplasts, while p35S-AC2 of EACMCV enhanced ACMV-[CM]

293 activity was measured by first transforming protoplasts with a DNA vector in a 96-well plate.

294 e constitutively or inducibly coexpressed in protoplasts with amiRNA candidates targeting single or m

295 Transfection of Arabidopsis orrm1 mutant protoplasts with constructs encoding a region encompassi

296 P fusion protein, extracted from Arabidopsis protoplasts, with 2'-O-methyloligonucleotide complementa

297 we could regenerate whole plants from edited protoplasts without employing selection.

298 e extracellular domain and in experiments in protoplasts without primary cell walls.

299 Herein, we tested if transient RNAi in protoplasts would result in the depletion of a targeted

300 When expressed alone in mesophyll protoplasts, ZmPIP2s are efficiently targeted to the pla