ライフサイエンスコーパス: protospacer

1 and the degradation of cognate invader DNA (protospacer).

2 uide the Cas9 nuclease to the viral targets (protospacers).

3 high specificity and efficiency for shorter protospacers.

4 the corresponding parts of viral DNA called protospacers.

5 d destruction of targets with fully matching protospacers.

6 -stranded RNA targets carrying complementary protospacers.

7 acent motif along with the first base of the protospacer (5'-AAG) partially affect the efficiency of

8 We find that the protospacer adjacent motif (PAM) affects primarily the R

9 udies have highlighted the importance of the protospacer adjacent motif (PAM) and a proximal 8-nucleo

10 that the S. aureus Cas9 recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA

11 9 cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to

12 Targeting requires a protospacer adjacent motif (PAM) and crRNA-DNA complemen

13 fied by guide RNA molecules and flanked by a protospacer adjacent motif (PAM) and is widely used for

14 Mutations in the protospacer adjacent motif (PAM) and seed regions block

15 guide RNA but also require recognition of a protospacer adjacent motif (PAM) by the Cas9 protein.

16 gitidis (NmCas9) recognizes a 5'-NNNNGATT-3' protospacer adjacent motif (PAM) different from those re

17 on strictly requires the presence of a short protospacer adjacent motif (PAM) flanking the target sit

18 1) is limited by their requirement of a TTTV protospacer adjacent motif (PAM) in the DNA substrate.

19 Cas9-mediated cleavage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA

20 e, which strictly requires the presence of a protospacer adjacent motif (PAM) next to the target site

21 ition by all studied Cas9 enzymes requires a protospacer adjacent motif (PAM) next to the target site

22 , single-nucleotide mutations in the seed or protospacer adjacent motif (PAM) of the target sequence

23 cific manner, dependent on the presence of a Protospacer Adjacent Motif (PAM) on the target.

24 ight a proofreading mechanism beyond initial protospacer adjacent motif (PAM) recognition and RNA-DNA

25 he simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements.

26 A protospacer adjacent motif (PAM) sequence flanking targe

27 genome requires the presence of a 5'-NGG-3' protospacer adjacent motif (PAM) sequence immediately do

28 DNA immediately downstream from a 5'-CCN-3' protospacer adjacent motif (PAM) that is critical for in

29 res a specific nucleotide sequence, called a protospacer adjacent motif (PAM), for target recognition

30 nition of a short DNA sequence, known as the protospacer adjacent motif (PAM), next to and on the str

31 ed to recognize altered DNA sequences as the protospacer adjacent motif (PAM), thereby expanding the

32 lele-selective CRISPR/Cas9 strategy based on Protospacer Adjacent Motif (PAM)-altering SNPs to target

33 unction of distance and orientation from the protospacer adjacent motif (PAM).

34 e distal nucleotides, plus disruption of the protospacer adjacent motif (PAM).

35 A reveal that Cascade recognizes an extended protospacer adjacent motif (PAM).

36 ze is constrained by the need for a specific protospacer adjacent motif (PAM).

37 trinucleotide signature sequence called the protospacer adjacent motif (PAM).

38 require recognition of a short trinucleotide protospacer adjacent motif (PAM).

39 ain responsible for the interaction with the protospacer adjacent motif (PAM).

40 cleotide seed region in the sgRNA and an NGG protospacer adjacent motif (PAM).

41 s assay, we provide direct evidence that the protospacer adjacent motif along with the first base of

42 argets via protein-mediated recognition of a protospacer adjacent motif and complementary base pairin

43 AsCpf1 recognizes the 5'-TTTN-3' protospacer adjacent motif by base and shape readout mec

44 volution so as to alter the recognition of a protospacer adjacent motif by the Cas1-Cas2 complex, whi

45 hlight residues important in DNA binding and protospacer adjacent motif recognition.

46 as9 proteins is governed by binding first to protospacer adjacent motif sequences on DNA, which is fo

47 vided sequence, with user-specified types of protospacer adjacent motif, and number of mismatches all

48 Because of its distinct protospacer adjacent motif, the N. meningitidis CRISPR-C

49 indow of the RNA:DNA hybrid, neighboring the protospacer adjacent motif.

50 three CRISPR loci for which the identity of protospacer adjacent motifs (PAMs) was unknown until now

51 enome-wide including creating and destroying protospacer adjacent motifs (PAMs).

52 ity, including a further optimization of the protospacer-adjacent motif (PAM) of Streptococcus pyogen

53 and engineered Cas9 variants with different protospacer-adjacent motif (PAM) specificities to expand

54 nput query sequences, it searches gRNA by 3' protospacer-adjacent motif (PAM), and possible off-targe

55 upon introduction of mismatches proximal to protospacer-adjacent motif (PAM), demonstrating that mis

56 ays dictated by the presence or absence of a protospacer-adjacent motif (PAM).

57 rice genomic sites which are followed by the protospacer-adjacent motif (PAM).

58 tospacer immediately following the essential protospacer-adjacent motif.

59 ands and recognizes the 5'-NNNVRYM-3' as the protospacer-adjacent motif.

60 e lacking tracrRNA, and it utilizes a T-rich protospacer-adjacent motif.

61 to the 12-bases proximal to the guide strand protospacer-adjacent motif.

62 within the activating target RNA (rPAM [RNA protospacer-adjacent motif]).

63 re remarkably diverse, they commonly rely on protospacer-adjacent motifs (PAMs) as the first step in

64 anisms of action, where most systems rely on protospacer-adjacent motifs (PAMs) for DNA target recogn

65 ermed "priming." Here, by using a randomized protospacer and PAM library and high-throughput plasmid

66 phodiester backbone interactions between the protospacer and the proteins explain the sequence-nonspe

67 comes of CRISPR-Cas response to two kinds of protospacers are not caused by different structures form

68 DNA segments, termed protospacers, are integrated into the CRISPR array in a

69 e in the crRNA, but not on the presence of a protospacer-associated motif (PAM) in the target.

70 d Cas3, which includes five positions of the protospacer at 6-nt intervals that readily tolerate muta

71 PAM favors separation of a few PAM-proximal protospacer base pairs allowing initial target interroga

72 two Cas3 domains forming a groove where the protospacer binds to Cas1-Cas2.

73 esults provide insight into the structure of protospacer-bound type I Cas1-Cas2-3 adaptation complexe

74 sal, single or multiple mutations within the protospacer but outside the seed region do not lead to e

75 When a protospacer contains a neighboring target interference m

76 When a protospacer contains a spacer acquisition motif AAG, spa

77 When the crRNA spacer fully matches a protospacer, CRISPR interference-that is, target destruc

78 -Cas2 complex bound to cognate 33-nucleotide protospacer DNA substrates.

79 Protospacer DNA with free 3'-OH ends and supercoiled tar

80 inity of the crRNA-guided Cascade complex to protospacer DNA.

81 y direct Watson-Crick pairing with invasive 'protospacer' DNA, but how they avoid targeting the space

82 rmation, thus additionally destabilizing the protospacer duplex.

83 marily the R-loop association rates, whereas protospacer elements distal to the PAM affect primarily

84 recognition and expanding toward the distal protospacer end.

85 Binding to a protospacer flanked by a PAM recruits a nuclease-active

86 e demonstrate that Cas13b has a double-sided protospacer-flanking sequence and elucidate RNA secondar

87 Escherichia coli, a vast majority of plasmid protospacers generate spacers integrated in CRISPR casse

88 All other protospacers give rise to spacers oriented in both ways

89 only for a seven-nucleotide seed region of a protospacer immediately following the essential protospa

90 lies on the directional transcription of the protospacer in vivo.

91 cer sequence, Cascade-bound crRNA recognizes protospacers in foreign DNA, causing its destruction dur

92 A) whose spacer partially matches a segment (protospacer) in target DNA.

93 assay, Cas1-Cas2-3 processed and integrated protospacers independent of Cas3 activity.

94 s homologous to the Cas1 protein involved in protospacer integration by the CRISPR-Cas adaptive immun

95 ence-repeat junction which is the target for protospacer integration catalyzed by the Cas1-Cas2 adapt

96 foreign deoxyribonucleic acid referred to as protospacer is added to the CRISPR cassette and becomes

97 -length spacer occurs, which may enhance the protospacer locating efficiency of the E. coli Cascade c

98 nt mutations in protospacers, though not all protospacer mutations lead to escape.

99 pecific hybrid (R-loop) with its complement (protospacer) on an invading DNA while displacing the non

100 ng point mutations in the seed region of the protospacer or its adjacent motif (PAM), but hosts quick

101 Through engineering of a protospacer region of phage DMS3 to make it a target of

102 th up to 13 mutations throughout the PAM and protospacer region.

103 quence of small CRISPR RNA (crRNA) matches a protospacer sequence in the viral genome.

104 e, the repair outcomes are determined by the protospacer sequence rather than genomic context, indica

105 of an invader plasmid carrying the matching protospacer sequence.

106 crRNA targets is made equal, fully matching protospacers stimulate primed adaptation much more effic

107 from Pectobacterium atrosepticum with bound protospacer substrate DNA.

108 entarity between CRISPR spacer RNA and phage protospacer target.

109 ISPR/Cas resistance carry point mutations in protospacers, though not all protospacer mutations lead

110 length of the DNA and splays the ends of the protospacer to allow each terminal nucleophilic 3'-OH to

111 ity, and (2) further unwinding of the entire protospacer to form a full R-loop.

112 sequences that are required upstream of the protospacer to permit target DNA recognition.

113 attack by a virus with mutated corresponding protospacers, while an excessive variety of spacers dilu

114 tegration required at least partially duplex protospacers with free 3'-OH groups, and leader-proximal

115 ge life style, the positions of the targeted protospacer within the genome, and the state of phage DN