ライフサイエンスコーパス: protospacer adjacent motif

1 indow of the RNA:DNA hybrid, neighboring the protospacer adjacent motif.

2 ands and recognizes the 5'-NNNVRYM-3' as the protospacer-adjacent motif.

3 e lacking tracrRNA, and it utilizes a T-rich protospacer-adjacent motif.

4 to the 12-bases proximal to the guide strand protospacer-adjacent motif.

5 tospacer immediately following the essential protospacer-adjacent motif.

6 within the activating target RNA (rPAM [RNA protospacer-adjacent motif]).

7 s assay, we provide direct evidence that the protospacer adjacent motif along with the first base of

8 argets via protein-mediated recognition of a protospacer adjacent motif and complementary base pairin

9 vided sequence, with user-specified types of protospacer adjacent motif, and number of mismatches all

10 AsCpf1 recognizes the 5'-TTTN-3' protospacer adjacent motif by base and shape readout mec

11 volution so as to alter the recognition of a protospacer adjacent motif by the Cas1-Cas2 complex, whi

12 We find that the protospacer adjacent motif (PAM) affects primarily the R

13 udies have highlighted the importance of the protospacer adjacent motif (PAM) and a proximal 8-nucleo

14 that the S. aureus Cas9 recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA

15 9 cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to

16 Targeting requires a protospacer adjacent motif (PAM) and crRNA-DNA complemen

17 fied by guide RNA molecules and flanked by a protospacer adjacent motif (PAM) and is widely used for

18 Mutations in the protospacer adjacent motif (PAM) and seed regions block

19 guide RNA but also require recognition of a protospacer adjacent motif (PAM) by the Cas9 protein.

20 gitidis (NmCas9) recognizes a 5'-NNNNGATT-3' protospacer adjacent motif (PAM) different from those re

21 on strictly requires the presence of a short protospacer adjacent motif (PAM) flanking the target sit

22 1) is limited by their requirement of a TTTV protospacer adjacent motif (PAM) in the DNA substrate.

23 Cas9-mediated cleavage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA

24 e, which strictly requires the presence of a protospacer adjacent motif (PAM) next to the target site

25 ition by all studied Cas9 enzymes requires a protospacer adjacent motif (PAM) next to the target site

26 , single-nucleotide mutations in the seed or protospacer adjacent motif (PAM) of the target sequence

27 cific manner, dependent on the presence of a Protospacer Adjacent Motif (PAM) on the target.

28 ight a proofreading mechanism beyond initial protospacer adjacent motif (PAM) recognition and RNA-DNA

29 he simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements.

30 A protospacer adjacent motif (PAM) sequence flanking targe

31 genome requires the presence of a 5'-NGG-3' protospacer adjacent motif (PAM) sequence immediately do

32 DNA immediately downstream from a 5'-CCN-3' protospacer adjacent motif (PAM) that is critical for in

33 res a specific nucleotide sequence, called a protospacer adjacent motif (PAM), for target recognition

34 nition of a short DNA sequence, known as the protospacer adjacent motif (PAM), next to and on the str

35 ed to recognize altered DNA sequences as the protospacer adjacent motif (PAM), thereby expanding the

36 lele-selective CRISPR/Cas9 strategy based on Protospacer Adjacent Motif (PAM)-altering SNPs to target

37 unction of distance and orientation from the protospacer adjacent motif (PAM).

38 ze is constrained by the need for a specific protospacer adjacent motif (PAM).

39 trinucleotide signature sequence called the protospacer adjacent motif (PAM).

40 require recognition of a short trinucleotide protospacer adjacent motif (PAM).

41 ain responsible for the interaction with the protospacer adjacent motif (PAM).

42 cleotide seed region in the sgRNA and an NGG protospacer adjacent motif (PAM).

43 e distal nucleotides, plus disruption of the protospacer adjacent motif (PAM).

44 A reveal that Cascade recognizes an extended protospacer adjacent motif (PAM).

45 ity, including a further optimization of the protospacer-adjacent motif (PAM) of Streptococcus pyogen

46 and engineered Cas9 variants with different protospacer-adjacent motif (PAM) specificities to expand

47 nput query sequences, it searches gRNA by 3' protospacer-adjacent motif (PAM), and possible off-targe

48 upon introduction of mismatches proximal to protospacer-adjacent motif (PAM), demonstrating that mis

49 ays dictated by the presence or absence of a protospacer-adjacent motif (PAM).

50 rice genomic sites which are followed by the protospacer-adjacent motif (PAM).

51 three CRISPR loci for which the identity of protospacer adjacent motifs (PAMs) was unknown until now

52 enome-wide including creating and destroying protospacer adjacent motifs (PAMs).

53 re remarkably diverse, they commonly rely on protospacer-adjacent motifs (PAMs) as the first step in

54 anisms of action, where most systems rely on protospacer-adjacent motifs (PAMs) for DNA target recogn

55 hlight residues important in DNA binding and protospacer adjacent motif recognition.

56 as9 proteins is governed by binding first to protospacer adjacent motif sequences on DNA, which is fo

57 Because of its distinct protospacer adjacent motif, the N. meningitidis CRISPR-C