ライフサイエンスコーパス: pseudouridine

1 pair acceptor-TPsiC arm (where Psi indicates pseudouridine).

2 rupts the water-mediated interactions of the pseudouridine.

3 ible for modifying uridine13 in tRNA(Glu) to pseudouridine.

4 idine residues of rRNA by converting them to pseudouridine.

5 ns, uridines at position 39 were modified to pseudouridine.

6 cytoplasmic small subunit rRNA shown to lack pseudouridine.

7 wo modified nucleosides, 5-methyluridine and pseudouridine.

8 toxification of phosphorylated compounds and pseudouridine.

9 ted Gly-Gln dipeptide conjugated to 6'-amino-pseudouridine.

10 r transcriptome-wide quantitative mapping of pseudouridine.

11 e, and can catalyze methylation at the N1 of pseudouridine.

12 bound by PKR more efficiently than mRNA with pseudouridine.

13 ntial for enzyme-catalyzed formation of both pseudouridines.

14 A in trans on a rescue plasmid restored both pseudouridines.

15 MG) cap, ten 2'-O-methylated residues and 13 pseudouridines.

16 id produce pseudouridine 552 in 16S rRNA and pseudouridines 1199, 2605, and 2833 in 23S rRNA.

17 ferase H (RlmH) methylates 23S ribosomal RNA pseudouridine 1915 (Psi1915), which lies near the riboso

18 Of these, only pseudouridine 2605 is found naturally in either E. coli

19 ts gene product is the corresponding E. coli pseudouridine 2605 synthase.

20 Kinetic experiments confirmed that pseudouridine 2605 was the primary target.

21 We propose that YpuL is the B. subtilis pseudouridine 2633 (2605 in E. coli) synthase.

22 ould form 23 S RNA pseudouridine 746 or tRNA pseudouridine 32 in vivo, showing that this conserved as

23 t forms 23 S rRNA pseudouridine 746 and tRNA pseudouridine 32, was deleted in strains MG1655 and BL21

24 ation of 23 S RNA pseudouridine 746 and tRNA pseudouridine 32.

25 rm either 23 S RNA pseudouridine 746 or tRNA pseudouridine 32.

26 Pseudouridine 35 (psi35) in the branch site recognition

27 zing RNAs up to 1.7 kb long as well as fully pseudouridine-, 5-methyl-C-, 2'-fluoro-, or 2'-azido-mod

28 The protein did not form pseudouridine 516 as expected but did produce pseudourid

29 rsuA, the gene for the synthase which forms pseudouridine 516 in Escherichia coli 16S rRNA, was clon

30 Pseudouridine 55 synthase (Psi55S) catalyzes isomerizati

31 of Thermotoga maritima and Escherichia coli pseudouridine 55 synthase (Psi55S) mutants in which the

32 Comparison of pseudouridine 55 synthase and tRNA (m5U54)-methyltransfe

33 Escherichia coli tRNA pseudouridine 55 synthase catalyzes pseudouridine format

34 Here we show that overexpression of the tRNA pseudouridine 55 synthase encoded by PUS4 also leads to

35 We conclude that recognition of tRNA by pseudouridine 55 synthase resides in the conformation of

36 However, pseudouridine 55 synthase was not stringent for a 7 base

37 ent to intact native tRNA as a substrate for pseudouridine 55 synthase, viz., the features for substr

38 ere used to analyze substrate recognition by pseudouridine 55 synthase.

39 seudouridine 516 as expected but did produce pseudouridine 552 in 16S rRNA and pseudouridines 1199, 2

40 douridine synthase RluA that forms 23 S rRNA pseudouridine 746 and tRNA pseudouridine 32, was deleted

41 nsible for the in vivo formation of 23 S RNA pseudouridine 746 and tRNA pseudouridine 32.

42 ated that neither mutant could form 23 S RNA pseudouridine 746 or tRNA pseudouridine 32 in vivo, show

43 letion mutant failed to form either 23 S RNA pseudouridine 746 or tRNA pseudouridine 32.

44 For example, an A-U, inosine*U and pseudouridine*A pair each form two hydrogen bonds.

45 To determine the sequence location of pseudouridine, a combination of enzymatic hydrolysis and

46 modified nucleosides m5C, m6A, m5U, s2U, or pseudouridine ablates activity.

47 trast, in vitro transcribed mRNAs containing pseudouridine activate PKR to a lesser degree, and trans

48 elomerase activity and the cellular level of pseudouridine, an H/ACA snoRNP-mediated modification of

49 Pseudouridine, an isomer of uridine, is probably the mos

50 site significantly distorts the flipped-out pseudouridine analogue such that a change in hybridizati

51 was used to generate the naturally occurring pseudouridine analogue.

52 es an enhanced, transcriptome-wide scope for pseudouridine and methods to dissect its underlying mech

53 ion of specific uridines in cellular RNAs to pseudouridines and may function as RNA chaperones.

54 Higher C-mannosyltryptophan, pseudouridine, and O-sulfo-L-tyrosine concentrations ass

55 cluding a 5' trimethylated guanosine cap, 13 pseudouridines, and 10 2'-O-methylated residues.

56 the role of PKR is validated by showing that pseudouridine- and uridine-containing RNAs were translat

57 Pseudouridines are proposed to enhance ribosome activity

58 recently identified C-mannosyltryptophan and pseudouridine as non-traditional kidney function markers

59 ndicated another major termination site: the pseudouridine at nucleotide 55.

60 ses catalyze the isomerization of uridine to pseudouridine at particular positions in certain RNA mol

61 on of the methyl group at the N3 position of pseudouridine at position 1915 causes a slight increase

62 The lack of pseudouridine at position 2504 of 23S rRNA was found to

63 Pseudouridine at position 39 (Psi(39)) of tRNA's anticod

64 e synthase 3 (Pus3), an enzyme known to form pseudouridine at positions 38 and 39 in yeast tRNA.

65 ase I catalyzes the conversion of uridine to pseudouridine at positions 38, 39, and/or 40 in the anti

66 NA(Leu)), there was very slight formation of pseudouridine at that position after incubation with mPu

67 the LC/MS/MS analysis that are indicative of pseudouridine at the 5' terminus (m/z 225 --> 165), inte

68 ymethyluridine, N6-isopentenyladenosine, and pseudouridine, at positions 34, 37, and 55, respectively

69 Messenger RNAs were not known to contain pseudouridine, but artificial pseudouridylation dramatic

70 uridines at specific sites are converted to pseudouridines by H/ACA ribonucleoprotein particles (RNP

71 a, uridines in various RNAs are converted to pseudouridines by RNA-guided RNA modification complexes

72 the enhanced translation of mRNAs containing pseudouridine, compared to those containing uridine, is

73 y was 0.78 for both C-mannosyltryptophan and pseudouridine concentration, and highly significant asso

74 h serum creatinine, C-mannosyltryptophan and pseudouridine concentrations showed little dependence on

75 The pseudouridine-containing hairpin is thermodynamically mo

76 e PKR to a lesser degree, and translation of pseudouridine-containing mRNAs is not repressed.

77 the characteristic dissociation reactions of pseudouridine-containing oligonucleotides following ioni

78 165, 164, 139), which permit recognition of pseudouridine-containing oligonucleotides.

79 acking interactions mediated by the U2 snRNA pseudouridines correlate with the identity of the unpair

80 s between viral RNA and tRNA(Lys3) thymidine-pseudouridine-cytidine and anticodon loops decreased the

81 7-nt element; (ii) loss of the 3' hairpin or pseudouridine does not affect rRNA processing; (iii) a s

82 This pseudouridine effect can also be applied to other pre-mR

83 Given that pseudouridine favors a C-3'-endo structure, our results

84 Here, the importance of pseudouridine formation (Psi) in the peptidyl transferas

85 nd five of these were verified as guides for pseudouridine formation at specific sites in ribosomal R

86 RNA pseudouridine synthase, TruB, catalyzes pseudouridine formation at U55 in tRNA.

87 oli tRNA pseudouridine 55 synthase catalyzes pseudouridine formation at U55 in tRNA.

88 of modifications remain unclear, such as for pseudouridine formation in the tRNA TPsiC arm by the bac

89 we propose a Michael addition mechanism for pseudouridine formation that is consistent with all expe

90 olar RNAs (snoRNAs), most of which guide RNA pseudouridine formation.

91 sion of stereochemistry at C2' suggests that pseudouridine generation may proceed by a mechanism invo

92 We show that AlnA and AlnB, members of the pseudouridine glycosidase and haloacid dehalogenase enzy

93 to the recognition and sequence placement of pseudouridine has not been straightforward, particularly

94 enhanced when its uridines are replaced with pseudouridines; however, the reason for this enhancement

95 This approach to pseudouridine identification is demonstrated using Esche

96 ide, single-nucleotide-resolution method for pseudouridine identification.

97 All pseudouridines identified in RNA are considered constitu

98 (RNPs) are responsible for the formation of pseudouridine in a variety of RNAs and are essential for

99 on of the transcriptome-wide distribution of pseudouridine in human and the factors governing it and

100 a class of enzymes that isomerize uridine to pseudouridine in noncoding RNAs, such as tRNA, to ensure

101 t post-transcriptionally modified nucleoside pseudouridine in nucleic acids has been developed.

102 ethod allows for the direct determination of pseudouridine in nucleic acids, can be used to identify

103 ses catalyze the isomerization of uridine to pseudouridine in RNA molecules.

104 n that catalyzes isomerization of uridine to pseudouridine in target RNAs.

105 We report the existence of pseudouridine in the anticodon of Escherichia coli tyros

106 Notably, the majority of pseudouridines in mRNA are regulated in response to envi

107 H/ACA RNP complexes change uridines to pseudouridines in target non-coding RNAs in eukaryotes a

108 More specifically, pseudouridines in the single-stranded loop regions of th

109 ffectively blocks the formation of important pseudouridines in U2 snRNA, as only a trace of pseudouri

110 equence, which is converted to GTPsiC (Psi = pseudouridine) in most tRNAs.

111 Using pseudouridine incorporation and in vivo RNA-guided RNA p

112 nosyltryptophan and 76.0% (68.6%, 82.4%) for pseudouridine, indicating partial net reabsorption.

113 equence, one can site-specifically introduce pseudouridines into virtually any RNA (e.g., mRNA, ribos

114 The C-nucleoside pseudouridine is a natural component of RNA, and various

115 eudouridines in U2 snRNA, as only a trace of pseudouridine is detected when cells are exposed to a lo

116 Pseudouridine is found in almost all cellular ribonuclei

117 Pseudouridine is the most abundant RNA modification, yet

118 mRNA containing the nucleoside modification pseudouridine is translated longer and has an extended h

119 Conversion of either uridine into pseudouridine leads to a splicing defect in Xenopus oocy

120 D-domain with the T-domain was enhanced by a pseudouridine located in either the D or T-domains compa

121 f the multiple characteristics attributed to pseudouridine, making messenger RNAs (mRNAs) highly tran

122 ition, supporting its role as an N1-specific pseudouridine methyltransferase.

123 otein required both for ribosomal RNA (rRNA) pseudouridine modification and for cellular accumulation

124 ure senescence support normal levels of rRNA pseudouridine modification and normal kinetics of rRNA p

125 ction and verification of snoRNAs that guide pseudouridine modification at more than two sites.

126 The structural effects of the pseudouridine modification at position 39 were investiga

127 des a pseudouridine synthase responsible for pseudouridine modification of 23S rRNA at three sites, a

128 erences may contribute to the ability of the pseudouridine modification to promote the bulged conform

129 Pseudouridine-modification of P6.1 slightly attenuates t

130 The three conserved pseudouridine modifications (Psi1911, Psi1915, Psi1917)

131 Our main findings are that pseudouridine modifications exhibit a range of effects o

132 With this work, 41 of the 44 known pseudouridine modifications in S.cerevisiae rRNA have be

133 f hairpin RNAs containing single or multiple pseudouridine modifications in the stem or loop regions.

134 esented to allow identification of MS-silent pseudouridine modifications.

135 temperature, magnesium, and the presence of pseudouridine modifications.

136 the A+-C base-pair increases the Tm of both pseudouridine modified and unmodified RNA hairpins by 5-

137 A pseudouridine-modified region of the U2 small nuclear (s

138 ne, guanosine, uridine, inosine, xanthosine, pseudouridine, N(2)-methylguanosine, 1-methyladenosine,

139 ney function measures: C-mannosyltryptophan, pseudouridine, N-acetylalanine, erythronate, myo-inosito

140 Here, we demonstrate that N1-methyl-pseudouridine (N1mPsi) outperforms several other nucleos

141 synthesis of a 5'-O-BzH-2'- O -ACE-protected pseudouridine phosphoramidite is reported [BzH, benzhydr

142 or the two hypermodified nucleosides and for pseudouridine phosphoramidite were all greater than 98%.

143 Isomerization from uridine to pseudouridine (pseudouridylation) is largely catalyzed b

144 onylcarbamoyladenosine (ms(2)t(6)A(37)), and pseudouridine (Psi(39)) in the tRNA's anticodon domain a

145 ed the anticodon domain modified nucleosides pseudouridine (Psi(39)), 5-methylaminomethyluridine (mnm

146 s N(6)-dimethylallyl adenine (i(6)A(37)) and pseudouridine (psi(39)).

147 er, modification of the initial uridine to a pseudouridine (Psi) allows efficient recognition and rea

148 ized structure-stabilizing RNA modifications pseudouridine (Psi) and 2'-O-methylation to determine if

149 ligation-based detection and quantitation of pseudouridine (Psi) and N6-methyladenosine (m6A), two ab

150 phosphoramidite was used in combination with pseudouridine (Psi) and standard base phosphoramidites t

151 C) outperformed the current state-of-the-art pseudouridine (Psi) and/or m5C/Psi-modified mRNA platfor

152 2-thiouridine at position 34 (mcm5s2U34) and pseudouridine (psi) at position 39--two of which, ms2t6A

153 S) catalyzes isomerization of uridine (U) to pseudouridine (Psi) at position 55 in transfer RNA.

154 d the tRNAs were assayed for the presence of pseudouridine (Psi) at the expected positions.

155 ermodynamic data are reported revealing that pseudouridine (Psi) can stabilize RNA duplexes when repl

156 lements of human tRNA(Ser) are necessary for pseudouridine (Psi) formation at position 28 in the anti

157 ethylation of the first four nucleotides and pseudouridine (psi) formation at uracil 28.

158 Many or all of the sites of pseudouridine (Psi) formation in eukaryotic rRNA are sel

159 RluB catalyses the modification of U2605 to pseudouridine (Psi) in a stem-loop at the peptidyl trans

160 r catalyzing the isomerization of uridine to pseudouridine (Psi) in ribosomal and other cellular RNAs

161 nosine (m(6)A), 5-methylcytosine (m(5)C) and pseudouridine (Psi) in RNA, and describe how these RNA m

162 ses (psi synthases) isomerize uridine (U) to pseudouridine (psi) in RNA, and they fall into five fami

163 Pseudouridine (Psi) is the most abundant internal modifi

164 a pseudouridine synthase responsible for the pseudouridine (Psi) modifications at positions 1911, 191

165 Three pseudouridine (Psi) modifications clustered in H69 are c

166 contains an unusually dense cluster of 8-10 pseudouridine (Psi) modifications located in a three-hel

167 ite showed that a phylogenetically conserved pseudouridine (psi) residue in the segment of U2 snRNA t

168 Replacing the uridine in CCUG repeats with pseudouridine (Psi) resulted in a modest reduction of MB

169 s recessive mutations in PUS1, which encodes pseudouridine (Psi) synthase 1 (Pus1p).

170 Human pseudouridine (Psi) synthase Pus1 (hPus1) modifies speci

171 VR1]) for a chloroplast-localized homolog of pseudouridine (Psi) synthase, which isomerizes uridine t

172 l nucleolar protein Cbf5p is the most likely pseudouridine (Psi) synthase.

173 Pseudouridine (Psi) synthases catalyze the formation of

174 Pseudouridine (Psi) synthases catalyze the isomerization

175 Several putative Escherichia coli pseudouridine (Psi) synthases have been identified by it

176 Pseudouridine (Psi) was recently established to be wides

177 udouridine synthases isomerize (U) in RNA to pseudouridine (Psi), and the mechanism that they follow

178 seudouridylation (conversion of uridine into pseudouridine (Psi), ref. 4) of nonsense codons suppress

179 Pseudouridine (psi), the most abundant of the modified b

180 osine (m(6)A), 5-methylcytosine (m(5)C), and pseudouridine (Psi).

181 leophile for the PsiS-catalyzed formation of pseudouridine (Psi).

182 Uridines 56 and 93 are both modified to pseudouridines (Psi) during nutrient deprivation, while

183 Briefly, we mapped pseudouridines (Psi) on rRNA by Psi-seq in procyclic for

184 modifications 2-thiouridine, s(2)U(34), and pseudouridine, Psi(39), appreciably stabilized the inter

185 U2 small nuclear RNA (snRNA) contains three pseudouridines (Psi35, Psi42, and Psi44).

186 The isomerization of up to 100 uridines to pseudouridines (Psis) in eukaryotic rRNA is guided by a

187 Forty-four of the 46 pseudouridines (Psis) in the cytoplasmic rRNA of Sacchar

188 ns modified nucleotides, including conserved pseudouridines (Psis) that can have subtle effects on st

189 sensitivity, and further, more than a single pseudouridine residue is involved, as alteration of sing

190 leic acids, can be used to identify modified pseudouridine residues and can be used with general modi

191 ity are approximately additive when multiple pseudouridine residues are present.

192 Pseudouridine residues can be identified in intact nucle

193 s helix 69 of 23S rRNA, which contains three pseudouridine residues in its loop region.

194 herichia coli 23S rRNA were synthesized with pseudouridine residues located at positions 1911, 1915 a

195 n units of 252 Da) will denote the number of pseudouridine residues present.

196 three different structural contexts for the pseudouridine residues were examined and compared with t

197 Adjacent pseudouridine residues were found in the single-stranded

198 After chemical derivatization all pseudouridine residues will contain a 252 Da 'mass tag'

199 RluD catalyses formation of three pseudouridine residues within helix 69 of the 50S riboso

200 2-morpholinoethyl)carbodiimide to derivatize pseudouridine residues.

201 transcriptional modifications of RNA, except pseudouridine, result in a mass increase in the canonica

202 in vitro using modified uridine 2' fluoro or pseudouridine ribonucleotides lacked signaling activity

203 mic stability of the RNA hairpin relative to pseudouridine; RNAs containing either uridine or 3-methy

204 d by gene disruption and loss of the cognate pseudouridine site.

205 new base-pairings between snoRNAs and known pseudouridine sites in S.cerevisiae rRNA, 12 of which we

206 Comparison of the four pseudouridine sites yielded a consensus recognition sequ

207 This negative impact on splicing is pseudouridine specific, as no effect is observed when th

208 by multiple detection methods, which include pseudouridine-specific chemical derivatization and gas p

209 rRNA pseudouridine stoichiometries are conserved but reduced

210 of macrophages with a F. tularensis LVS rluD pseudouridine synthase (FTL_0699) mutant resulted in dim

211 We identified a pseudouridine synthase (PUS), mPus1p, as a coactivator f

212 Pseudouridine synthase 1 (Pus1p) is an unusual site-spec

213 The PUS1 gene encodes the enzyme pseudouridine synthase 1 (Pus1p) that is known to pseudo

214 tified a homozygous missense mutation in the pseudouridine synthase 1 gene (PUS1) in all patients wit

215 A cDNA encoding mouse pseudouridine synthase 3 (mPus3p) has been cloned.

216 redicted protein has 34% identity with yeast pseudouridine synthase 3 (Pus3), an enzyme known to form

217 Pseudouridine Synthase 4 (Pus4) and the Actin Patch Prot

218 de that the conserved Asp60 is essential for pseudouridine synthase activity and propose mechanisms w

219 r novel domain, designated PUA domain, after PseudoUridine synthase and Archaeosine transglycosylase,

220 rate bound to the ribonucleoprotein particle pseudouridine synthase and enzyme activity assay confirm

221 Ten methyltransferases and one pseudouridine synthase are required for complete modific

222 ific H/ACA RNA and four common proteins, the pseudouridine synthase Cbf5, Nop10, Gar1, and Nhp2.

223 stand alone pseudouridine synthases, the RNP pseudouridine synthase comprises multiple protein subuni

224 ight of the global dissimilarity between the pseudouridine synthase families, we changed the aspartic

225 ture of the RNA-modifying enzyme, psi55 tRNA pseudouridine synthase from Mycobacterium tuberculosis,

226 our findings also support the assignment of pseudouridine synthase function to certain physiological

227 tic acid residue might be a prerequisite for pseudouridine synthase function.

228 tRNA pseudouridine synthase I (PsiSI) catalyzes the conversio

229 tRNA pseudouridine synthase I catalyzes the conversion of uri

230 TruD, a recently discovered novel pseudouridine synthase in Escherichia coli, is responsib

231 Analysis of total tRNA isolated from E. coli pseudouridine synthase knock-out mutants identified RluF

232 pseudouridine synthases (PUS) uncovers which pseudouridine synthase modifies each site and their targ

233 e caused by mostly missense mutations in the pseudouridine synthase NAP57 (dyskerin/Cbf5).

234 The rluC gene encodes a pseudouridine synthase responsible for pseudouridine mod

235 The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine

236 e Escherichia coli gene rluA, coding for the pseudouridine synthase RluA that forms 23 S rRNA pseudou

237 Pseudouridine synthase RluE modifies U2457 in a stem of

238 Escherichia coli pseudouridine synthase RluF is dedicated to modifying U2

239 The DKC1 gene encodes a pseudouridine synthase that modifies ribosomal RNA (rRNA

240 Mutations in DKC1, encoding for dyskerin, a pseudouridine synthase that modifies rRNA and regulates

241 ar ribonucleoprotein complexes and acts as a pseudouridine synthase to modify newly synthesized ribos

242 Such a role for cysteine in the pseudouridine synthase TruA (also named Psi synthase I)

243 icodon stem loop (ASL) by a highly conserved pseudouridine synthase TruA.

244 The pseudouridine synthase TruB handles 5-fluorouridine in R

245 we prove the tRNA chaperone activity of the pseudouridine synthase TruB, reveal its molecular mechan

246 in the tRNA TPsiC arm by the bacterial tRNA pseudouridine synthase TruB.

247 he downstream genes ppnK (NAD kinase), rluE (pseudouridine synthase), and pta (phosphotransacetylase)

248 guide RNA and four essential proteins: Cbf5 (pseudouridine synthase), L7Ae, Gar1 and Nop10 in archaea

249 Dyskerin is a putative pseudouridine synthase, and it has been suggested that D

250 roteins, including ribosomal protein S4, RNA pseudouridine synthase, and tyrosyl-tRNA synthetase.

251 Nine methyltransferases, as well as the pseudouridine synthase, are already known.

252 The pseudouridine synthase, Cbf5, is also the protein that s

253 A guide RNA and four proteins, including the pseudouridine synthase, Cbf5.

254 e, TERC, and other components, including the pseudouridine synthase, dyskerin, the product of the DKC

255 idine, bound to a ribonucleoprotein particle pseudouridine synthase, strongly prefer the syn glycosid

256 RNA pseudouridine synthase, TruB, catalyzes pseudouridine fo

257 morphic alleles of nop60B, a gene encoding a pseudouridine synthase.

258 4, an uncharacterized mitochondrial putative pseudouridine synthase.

259 id residue is catalytically essential in one pseudouridine synthase.

260 antly alter the catalytic activity of either pseudouridine synthase.

261 f the TruA, TruB, RsuA, and RluA families of pseudouridine synthases (PsiS) identifies a strictly con

262 tructural comparisons with other families of pseudouridine synthases (PsiS) indicate that Psi55S may

263 Perturbing pseudouridine synthases (PUS) uncovers which pseudouridi

264 ery similar to the catalytic domain of other pseudouridine synthases and a second large domain (149 a

265 tural properties that are unique among known pseudouridine synthases and are consistent with its dist

266 roline residues in Motif I of RluA and TruB, pseudouridine synthases belonging to different families.

267 The pseudouridine synthases catalyze the isomerization of ur

268 The pseudouridine synthases catalyze the isomerization of ur

269 ue is critical for the catalytic activity of pseudouridine synthases from two additional families of

270 de a resource for identifying the targets of pseudouridine synthases implicated in human disease.

271 s little sequence homology with the other 10 pseudouridine synthases in E. coli which themselves have

272 The pseudouridine synthases isomerize (U) in RNA to pseudour

273 e alignments using the first four identified pseudouridine synthases led Koonin and, independently, S

274 ry to probe the role of cysteine residues in pseudouridine synthases of the families that do not incl

275 ence and structural comparisons suggest that pseudouridine synthases of the RluA, RsuA, and TruA fami

276 The predicted SwoCp is homologous to rRNA pseudouridine synthases of yeast (Cbf5p) and humans (Dkc

277 n that there are four distinct "families" of pseudouridine synthases that share no statistically sign

278 On the basis of sequence alignments, the pseudouridine synthases were grouped into four families

279 in the ribosomal protein S4, two families of pseudouridine synthases, a novel family of predicted RNA

280 ase, was detected in archaeal and eukaryotic pseudouridine synthases, archaeal archaeosine synthases,

281 modification sites to one of seven conserved pseudouridine synthases, Pus1-4, 6, 7 and 9.

282 an active site cleft, conserved in all other pseudouridine synthases, that contains invariant Asp and

283 Different from stand alone pseudouridine synthases, the RNP pseudouridine synthase

284 Asp60, conserved in all known and putative pseudouridine synthases, was mutated to amino acids with

285 ficant relative to turnover by the wild-type pseudouridine synthases.

286 to these previously identified ribosomal RNA pseudouridine synthases.

287 ends on both site-specific and snoRNA-guided pseudouridine synthases.

288 uridine (or pseudouridylation) catalyzed by pseudouridine synthases.

289 However, neither rRNA pseudouridine synthesis nor rRNA processing appears to b

290 RNA methylases, a yeast protein containing a pseudouridine synthetase and a deaminase domain, bacteri

291 ssembly factors, such as helicases, GTPases, pseudouridine synthetases, and methyltransferases, are a

292 Pseudouridine, the most abundant modified nucleoside in

293 the C-C (rather than C-N) glycosidic bond of pseudouridine, the otherwise common dissociation paths i

294 nce of one-third of the normal complement of pseudouridines, there was no change in the exponential g

295 52 Da 'mass tag' that allows the presence of pseudouridine to be identified using mass spectrometry.

296 are used to narrow the sequence location of pseudouridine to specific T1 fragments in the gene seque

297 the top half domain composed of acceptor and pseudouridine (TPsiC) arms is more ancient than the bott

298 Pseudouridine was verified by multiple detection methods

299 The respective concentrations for pseudouridine were 2.89/5.67 micromol/L and 39.7/33.9 mi

300 sine bulge is associated with a well-stacked pseudouridine, which is linked via an ordered water mole