ライフサイエンスコーパス: psoralen

1 ICLs) induced by drugs such as cisplatin and psoralen.

2 t to angelicin but eliminated recruitment to psoralen.

3 henylphosphine oxide-1, or 5-(4-phenylbutoxy)psoralen.

4 ific photoadduct formation by the conjugated psoralen.

5 on, we tested dimeric bis-PNAs conjugated to psoralen.

6 account for some of the mutations induced by psoralens.

7 Psoralen 4 (Pso4) is an evolutionarily conserved protein

8 immunosuppressant PAP-1 (5-(4-phenoxybutoxy)psoralen), a high-affinity blocker of Kv1.3 channels in

9 The assay is based on mutagenesis by psoralen, a photoactive DNA cross-linker.

10 t, largely accounting for the differences in psoralen accessibility between active and inactive genes

11 l are transcribed and maintained in an open, psoralen-accessible conformation.

12 enhanced effectiveness of the clamp ODNs to psoralen activation.

13 inked to psoralen, have been shown to direct psoralen adduct formation in cells, leading to mutagenes

14 , neither XPA nor XPC is cross-linked to the psoralen adduct.

15 nd are required for XPD cross-linking to the psoralen-adducted base, neither XPA nor XPC is cross-lin

16 excision nuclease assembled at the site of a psoralen-adducted thymine.

17 We previously demonstrated that psoralen adducts targeted by triple-helix-forming oligon

18 Removal of psoralen adducts was blocked in XPC-deficient cells but

19 , a more rapid removal of digoxigenin-tagged psoralen adducts, and decreased cellular sensitivity to

20 e helices, even in the absence of associated psoralen adducts, are able to provoke DNA repair and cau

21 t mutations induced by third-strand-directed psoralen adducts.

22 XPC protein was rapidly recruited to psoralen adducts.

23 In addition to the ICL formation, psoralens also readily form various monoadducts (MAs) wi

24 or pathogen inactivation using the synthetic psoralen amotosalen HCl.

25 re densely biotinylated using a biotinylated psoralen analog plus UVA light and hybridized on microar

26 NEIL3 is the primary unhooking mechanism for psoralen and abasic site ICLs.

27 further characterized the inhibition of four psoralen and coumarin derivatives toward ALDH2 and compa

28 The TFOs were linked to psoralen and designed to target and mutagenize a site in

29 Psoralen and its derivatives are widely applied for the

30 sensitive to photochemical inactivation with psoralen and long-wavelength ultraviolet light.

31 he collagenase gene upon treatment with free psoralen and subsequent photoactivation.

32 In this study, APS165 was modified with psoralen and the extent of triplex formation with alpha1

33 tation equilibrium of 3'-linked intercalated psoralen and to develop conditions that lead to preferen

34 Sunlight and psoralen and ultraviolet A (PUVA) are risk factors for t

35 Psoralen and ultraviolet A light (PUVA) are used to kill

36 es that themselves had never been exposed to psoralen and ultraviolet A radiation.

37 king the ciclosporin who had been exposed to psoralen and ultraviolet-A light (PUVA) and other treatm

38 elix-forming oligonucleotides were linked to psoralen and used to form triplexes on the plasmids.

39 tive to photochemical inactivation with S-59 psoralen and UV light.

40 om 30 minute reactions were crosslinked with psoralen and UV, linearized with restriction enzymes and

41 with photochemical therapy (PCT) using S-59 psoralen and UVA light to prevent proliferation.

42 s are widely consumed foods that are rich in psoralens and furocoumarins, a group of naturally occurr

43 A combination of psoralens and ultraviolet A radiation is widely used to

44 patients with severe psoriasis treated with psoralens and ultraviolet-A therapy (PUVA) who enrolled

45 from Xenopus laevis has been shown to cleave psoralen- and abasic site-induced ICLs in Xenopus egg ex

46 ary using this system identified the angular psoralen angelicin and the insect growth regulator fenox

47 DNA damage, thus ICL-inducing agents such as psoralen, are clinically useful chemotherapeutics.

48 Upon near-ultraviolet irradiation (360 nm), psoralen-Asp41-Tat(37-72) cross-linked to a single site

49 The psoralen-bis-PNA conjugate was found to direct photoaddu

50 ks was dependent on precise placement of the psoralen by the triple helix-forming oligonucleotide at

51 Photoreactive psoralens can form interstrand crosslinks (ICLs) in doub

52 Upon exposure to UVA light, psoralens can induce DNA interstrand cross-links (ICLs),

53 s (TFOs) linked to DNA damaging agents (e.g. psoralen) can stimulate HR, providing the potential to i

54 Triplex formation by an oligonucleotide-psoralen conjugate was used to form a covalent photoaddu

55 We show that psoralen-conjugated pcPNAs can deliver site-specific pho

56 imethylaminopropylamine, in a 10-nucleotide, psoralen-conjugated TFO confers substantial increases in

57 Using a psoralen-conjugated TFO designed to bind to a site overl

58 was induced efficiently by injection of both psoralen-conjugated TFOs (followed by long-wave UVA ligh

59 Psoralen-conjugated triplex-forming oligonucleotides (ps

60 Interactions between nuclease-resistant, 5'-psoralen-conjugated, chimeric methylphosphonate oligodeo

61 of pyrimidine deoxyribo- or 2'-O-methylribo-psoralen-conjugated, triplex-forming oligonucleotides, p

62 These results indicate that TFO-linker-psoralen conjugates allow simultaneous, efficient target

63 ree, two, or no strands covalently linked by psoralen conjugation and photoaddition.

64 A defined template DNA with a single psoralen cross-link and the SV40 origin of replication w

65 The psoralen cross-link defines the intramolecular interacti

66 The psoralen cross-link inhibited DNA replication by termina

67 ation of TBP from DNA was not prevented by a psoralen cross-link positioned immediately preceding the

68 The major pathway of psoralen cross-link repair in yeast is error-free and in

69 ontaining four adjacent cytosines, next to a psoralen cross-link site.

70 A site-specific psoralen cross-link was placed between the promoter and

71 porter gene, we showed that a single defined psoralen cross-link was removed in repair-proficient cel

72 l incisions 5' to the cross-linked base of a psoralen cross-link, generating a gap of 22 to 28 nucleo

73 We compared psoralen cross-link, psoralen monoadduct, and DSB repair

74 epair of plasmid molecules carrying a single psoralen cross-link, psoralen monoadduct, or double-stra

75 UV-induced psoralen cross-linking is used to probe the secondary st

76 Nuclease digestion and psoralen cross-linking mapped the PIC between positions

77 understand the relationship between triplex psoralen cross-linking products and the fate of RNA Pol

78 ed after UV irradiation, which initiates the psoralen cross-linking reaction.

79 These studies have low sensitivity, because psoralen cross-linking suggested few (estimated <10%) of

80 embedded sequences for triplex formation and psoralen cross-linking.

81 e characterized by elevated accessibility to psoralen cross-linking.

82 In the yeast Saccharomyces cerevisiae, psoralen cross-links are processed by nucleotide excisio

83 Our observations suggest that psoralen cross-links can be repaired by three pathways:

84 Psoralen cross-links induced both recombination and muta

85 comparison, the ability of triplex-directed psoralen cross-links to induce recombination was only pa

86 Site-specific mutagenesis by psoralen cross-links was dependent on precise placement

87 repair of laser-localized lesions formed by psoralen (cross-links/monoadducts) and angelicin (only m

88 Inhibition of transcription by psoralen-cross-linked triplexes results in both arrest a

89 x DNA containing a site-directed interstrand psoralen crosslink.

90 Chloroquine titration, psoralen crosslinking and supercoiling-sensitive reporte

91 develop PARIS, a method based on reversible psoralen crosslinking for global mapping of RNA duplexes

92 A light-induced psoralen crosslinking reaction was used to attach a puro

93 ve fraction from yeast nuclei, and selective psoralen crosslinking was used to distinguish between ac

94 ld be detected only after analysis following psoralen crosslinking.

95 demonstrate that TFOs can be used to direct psoralen crosslinks adjacent to a gene as a way of activ

96 protection assay to detect triplex-directed psoralen crosslinks in genomic DNA prepared from TFO-tra

97 NEIL1 recruitment to psoralen crosslinks was independent of the nucleotide ex

98 blocked by infrequent, randomly distributed psoralen crosslinks, but the bacterial population was ab

99 se that RPA70 makes the initial contact with psoralen-damaged DNA but that within preincision complex

100 omplex is formed, containing HMGB1, RPA, and psoralen-damaged DNA.

101 Using a psoralen delivery system mediated by a DNA third strand

102 Some new psoralen derivatives were synthesized and evaluated as i

103 Among the psoralen derivatives, 8-methoxypsoralen (8-MOP) is conve

104 chemical cross-linking of DNA to immobilized psoralen derivatives.

105 A glycosylases Nei and NEIL1 excise unhooked psoralen-derived ICLs in three-stranded DNA via hydrolys

106 lusion, the Nei-like DNA glycosylases unhook psoralen-derived ICLs in various DNA structures via a ge

107 ikingly, crosslinking of the siRNA duplex by psoralen did not completely block RNA interference, indi

108 WRN facilitates ATM activation in cells with psoralen DNA cross-link-induced collapsed replication fo

109 n control cells 6 and 16 h after exposure to psoralen DNA cross-links.

110 ay of NER is critical for the recognition of psoralen DNA ICLs in the mammalian genome.

111 PA has also been suggested to play a role in psoralen DNA interstrand cross-link (ICL) repair, but a

112 ucts low-efficiency nonmutagenic bypass of a psoralen DNA interstrand cross-link (ICL), whose structu

113 age recognition proteins to triplex-directed psoralen DNA interstrand cross-links (ICLs).

114 -forming oligonucleotides (TFOs) to direct a psoralen-DNA interstrand cross-link (ICL) to a specific

115 TPE can initiate photochemistry resulting in psoralen-DNA photoadducts, target DNA sequences were inc

116 DNA from irradiated cells treated with psoralen exhibited a 4- to 7-fold increase in tritium ac

117 cs on platelet membrane extracts showed that psoralen forms adducts with unsaturated carbon bonds of

118 h purine and pyrimidine TFOs, when linked to psoralen, have been shown to direct psoralen adduct form

119 L with a greater efficiency than that of the psoralen ICL alone.

120 designed a model substrate DNA with a single psoralen ICL at a three-way junction (Y-shaped DNA), whi

121 f a DNA substrate containing a site-specific psoralen ICL can be monitored in vitro.

122 Using laser-induced psoralen ICL formation in cells, we find that FANCA inte

123 A and PCNA are involved in an early stage of psoralen ICL processing.

124 d MUS81-EME1 incises DNA at the 5' side of a psoralen ICL residing in fork structures.

125 generated a defined substrate with a single psoralen ICL that mimics a postincision structure genera

126 triplex technology to direct a site-specific psoralen ICL to a target DNA substrate to determine whet

127 the UvrABC nuclease cleaved the TFO-directed psoralen ICL with a greater efficiency than that of the

128 However, how TFO-directed psoralen ICLs (Tdp-ICLs) are recognized and processed in

129 1 (HMGB1) protein bound to triplex-directed psoralen ICLs (TFO-ICLs) in vitro, cooperatively with NE

130 ors believed to be involved in the repair of psoralen ICLs [xeroderma pigmentosum (XP)-A, XP-C, XP-F,

131 simultaneously, demonstrating not only that psoralen ICLs are recognized by XPC-hHR23B alone, but al

132 The induction of p53 phosphorylation by psoralen ICLs did not require factors believed to be inv

133 kade of transcription and DNA replication by psoralen ICLs during S-phase elicits a strong apoptotic

134 Here, we show that processing of psoralen ICLs in mammalian cell extracts is dependent up

135 The processing of psoralen ICLs is also dependent upon the nucleotide exci

136 protein complexes were also observed to bind psoralen ICLs simultaneously, demonstrating not only tha

137 binds with high affinity and specificity to psoralen ICLs, and interacts with the essential NER prot

138 eta complex is shown to specifically bind to psoralen ICLs, and this binding is stimulated by the add

139 sults demonstrate that XPC-hHR23B recognizes psoralen ICLs, which have a structure fundamentally diff

140 air (MMR) complex MutSbeta were sensitive to psoralen ICLs.

141 clease UvrABC plays a key role in processing psoralen ICLs.

142 f a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen

143 sing the docked poses of 29 (the most active psoralen in the series) as templates for alignment of th

144 nocytogenes as a model platform, recombinant psoralen-inactivated Lm DeltauvrAB vaccines induced pote

145 followed by G1 (G3/G1) VLPs, or with live or psoralen-inactivated SA11.

146 oxidative DNA damage, as well as trioxsalen (psoralen)-induced DNA interstrand crosslinks, but not to

147 Here we report that human NEIL3 cleaves psoralen-induced DNA-DNA cross-links in three-stranded a

148 rthermore, while Nei and NEIL1 also cleave a psoralen-induced four-stranded DNA substrate to generate

149 The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the firs

150 Psoralen-induced ICLs blocked transcription and replicat

151 produce monoadducts and ICLs and found that psoralen-induced ICLs induced phosphorylation of the Ser

152 CJ recruitment to laser-induced DSBs but not psoralen-induced ICLs is dependent on nuclease-active MR

153 l cycle arrest and genomic instability after psoralen-induced ICLs.

154 136R, and E181K, to oxidative DNA damage and psoralen-induced interstrand crosslinks was differential

155 p53 and apoptosis much more effectively than psoralen-induced monoadducts.

156 photoadducts directed by PNAs conjugated to psoralen-induced mutations at frequencies in the range o

157 In particular, using the N-4 linker, psoralen interaction with the residues of the non-coding

158 in production of incisions at the site of a psoralen interstrand cross-link and that in Fanconi anem

159 oduction of the 5' incision at the site of a psoralen interstrand cross-link as well as the 3' incisi

160 Here, we show that a single psoralen interstrand cross-link induces DNA synthesis in

161 ps of psoralen photoadducts: monoadducts and psoralen interstrand cross-link products.

162 ' and 3' incisions that occur at a site of a psoralen interstrand cross-link.

163 nuclei, specifically binds to DNA containing psoralen interstrand cross-links and that the FANCA, FAN

164 The repair of psoralen interstrand cross-links in the yeast Saccharomy

165 To elucidate factors affecting TFO-directed psoralen interstrand crosslink (ICL)-induced recombinati

166 vity, purified NEIL1 protein bound stably to psoralen interstrand crosslink-containing synthetic olig

167 cient in the stimulation of DNA synthesis by psoralen interstrand crosslinks.

168 Although the presence of psoralen is not required for targeting nor is it alone a

169 mage, are in close physical proximity to the psoralen lesion and thus are cross-linked to the damaged

170 XPF-ERCC1 complex makes an incision 5' to a psoralen lesion on Y-shaped DNA in a damage-dependent ma

171 the position of its subunits relative to the psoralen lesion.

172 r a 72 h period, suggesting that TFO-induced psoralen lesions are not repaired on this time scale.

173 ralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.

174 Psoralens linked to triplex-forming oligonucleotides (ps

175 We have constructed chemically modified, psoralen-linked TFOs that mediate site-specific mutagene

176 We have described psoralen-linked TFOs with 2'-O-methyl and 2'-O-(2-aminoe

177 We have prepared psoralen-linked TFOs with varying amounts of the AE-modi

178 site was greatly improved by shortening the psoralen linker.

179 We combined in vivo psoralen-mediated RNA-RNA crosslinking and high-throughp

180 o-severe disease if they had been prescribed psoralen, methotrexate, cyclosporine, acitretin, adalimu

181 at(42-72) and Tat(37-72), and incorporated a psoralen-modified amino acid at position 41 during solid

182 Psoralen-modified TFOs and UVA irradiation were used to

183 We have used psoralen-modified triplex-forming oligonucleotides (TFOs

184 Psoralen-modified triplex-forming oligonucleotides (TFOs

185 Psoralen-modified triplex-forming oligonucleotides (TFOs

186 onucleotide third strands with a 3'-terminal psoralen moiety attached by linkers that differ in lengt

187 for G-quadruplex binders characterized by a psoralen moiety.

188 igonucleotides attached with a photoreactive psoralen molecule (psoTFO) can be used to induce site-sp

189 We compared psoralen cross-link, psoralen monoadduct, and DSB repair using plasmid substr

190 cules carrying a single psoralen cross-link, psoralen monoadduct, or double-strand break in yeast cel

191 ence, the kinetics of formation and yield of psoralen monoadducts and crosslinks with pyrimidine resi

192 Target DNA acquired strand-specific psoralen monoadducts in a light dose-dependent fashion.

193 nnel blockers Margatoxin, 5-(4-Phenoxybutoxy)psoralen, or broad-spectrum K(+) channel blocker 4-Amino

194 compound of this series, 5-(4-phenoxybutoxy)psoralen (PAP-1), blocks Kv1.3 in a use-dependent manner

195 Most of the induced psoralen-pcPNA mutations were single-base substitutions

196 Such psoralen-peptide conjugates provide a new class of probe

197 derived from photoaffinity cross-linking by psoralen, phenphi (cis-Rh(1,10-phenanthroline)(9,10-phen

198 GM847, GM847-Tert, and WI-38 VA13 ALT cells, psoralen photo-cross-linked in situ, and the telomere re

199 te the mechanism of photoadduct-formation by psoralen photo-cross-linking, triplex structures were ge

200 Gene activation was dependent on psoralen photo-crosslinking.

201 These results indicate that psoralen photo-crosslinks play a prominent role in activ

202 Psoralen photoadduct formation was shown to be essential

203 eriments that monitored the formation of the psoralen photoadducts also suggested that the length of

204 Northern blots indicated that TFO-directed psoralen photoadducts blocked progression of RNA polymer

205 ate the mechanism for creating site-specific psoralen photoadducts in a target duplex DNA.

206 he only pathway that can metabolize targeted psoralen photoadducts into recombinagenic intermediates.

207 e mechanism associated with the formation of psoralen photoadducts that are directed by psoTFO during

208 soTFOs) were designed to deliver UVA-induced psoralen photoadducts to two distinct sequences within t

209 The formation of these psoralen photoadducts was also photoreversible through e

210 The psoralen photoadducts were then analyzed after UV irradi

211 DNA duplex generated two distinct groups of psoralen photoadducts: monoadducts and psoralen interstr

212 site-specific cross-linking method based on psoralen photochemistry to determine the effect of core

213 was investigated by micrococcal nuclease and psoralen photocrosslinking.

214 d for their ability to form triplex-directed psoralen photoproducts with both the mutant T residue of

215 Psoralen photoreacts with DNA to form interstrand cross-

216 ultimately offering the potential to define psoralen plus ultraviolet A dosage regimes in terms of m

217 nificantly altered the threshold response to psoralen plus ultraviolet A erythema but not the rate of

218 etectable effect on the maximum slope of the psoralen plus ultraviolet A erythema dose-response curve

219 aviolet-A-emitting fluorescent tubes used in psoralen plus ultraviolet A therapy.

220 Although psoralen plus ultraviolet A treatment is highly effectiv

221 tment with standardized green tea extract in psoralen plus ultraviolet A treatment populations abroga

222 ed green tea extract per cm2 30 min prior to psoralen plus ultraviolet A treatment resulted in an alm

223 the standard dose used in photochemotherapy (psoralen plus ultraviolet A).

224 Derm, a reconstituted human skin equivalent, psoralen plus ultraviolet A-induced 8-methoxypsoralen-DN

225 resulted in an almost complete abrogation of psoralen plus ultraviolet A-induced erythema.

226 violet A treatment populations abrogates the psoralen plus ultraviolet A-induced photochemical damage

227 to reduce the risk of cancer development in psoralen plus ultraviolet A-treated populations are high

228 traviolet A. c-fos and p53 positive cells in psoralen plus ultraviolet A-treated skin were found to b

229 sfully in combination with ultraviolet B and psoralen plus ultraviolet A.

230 ein induction following a single exposure to psoralen plus ultraviolet A. c-fos and p53 positive cell

231 Psoriatic patients undergoing psoralen plus ultraviolet radiation (PUVA) therapy are s

232 The Psoralen plus Ultraviolet-A (PUVA) cohort study has been

233 are hypersensitive to DNA damage induced by psoralen plus UVA irradiation (PUVA) or UVC radiation, s

234 Psoralen plus UVA light (PUVA) is commonly used to treat

235 argeting in the episomal shuttle vector, the psoralen-PNA-induced mutation frequency was 0.13%, 3.5-f

236 Using UV/psoralen (Ps)-inactivated virus to block viral transcrip

237 orming oligonucleotides (TFOs) that target a psoralen (pso) interstrand crosslink to a specific chrom

238 ple helix-forming oligonucleotides linked to psoralen (pso-TFO) to introduce a DNA interstrand cross-

239 helix-forming DNA oligonucleotides linked to psoralen (psoTFOs) were designed to deliver UVA-induced

240 a DNA interstrand crosslinking (ICL) agent, psoralen (pTFO-ICLs), to improve the gene targeting effi

241 and the KV1.3/1.5 blocker 5-(4-phenylbutoxy)psoralen reduced H2O2-elicited dilation to a similar ext

242 Photochemical treatment (PCT) with the psoralen S-59 and long wavelength ultraviolet light (UVA

243 We used these psoralen-Tat conjugates to form specific complexes with

244 Psoralen-TFO conjugates formed DNA cross-links with high

245 We conclude that directing DNA damage with psoralen-TFOs is an efficient and specific means for blo

246 of cells after transfection with plasmid and psoralen-TFOs produced photoadducts inside the cells and

247 gments containing either an abasic site or a psoralen-thymine monoadduct.

248 rate the ability of bis-PNAs conjugated with psoralen to mediate site-specific gene modification, and

249 UV-A and UV-B, when used in combination with psoralens, topical corticosteroids, vitamin D analogues,

250 unctional survival assays indicated that the psoralen-TPE treatment was not toxic to cells.

251 A tetrafluorphenyl (TFP) ester of trimethyl psoralen (trioxalen) was synthesized, and the procedure

252 e time of maximal erythema, the slope of the psoralen ultraviolet A dose-response curve was approxima

253 Peak psoralen ultraviolet A erythema, assessed by minimal pho

254 we determined that only 67% of mean maximum psoralen ultraviolet A erythemal intensity had developed

255 If assessment of psoralen ultraviolet A erythemal sensitivity had been ma

256 gs, it seems appropriate to consider whether psoralen ultraviolet A minimal phototoxic dose measureme

257 esponses to ultraviolet B, ultraviolet A and psoralen ultraviolet A were assessed visually and using

258 r to determine the separate contributions of psoralen + ultraviolet A and other ultraviolet exposures

259 here was some evidence that high exposure to psoralen + ultraviolet A before a first basal cell carci

260 Risk increased with high psoralen + ultraviolet A exposure prior to the first squ

261 s with low pre-first squamous cell carcinoma psoralen + ultraviolet A exposure, 95% confidence interv

262 Long-term, high-dose exposure to psoralen + ultraviolet A is associated with an increased

263 lop a squamous cell carcinoma after starting psoralen + ultraviolet A therapy should be closely monit

264 d 258 a basal cell carcinoma after beginning psoralen + ultraviolet A therapy.

265 rates of post-first squamous cell carcinoma psoralen + ultraviolet A treatment also were associated

266 Higher post-first tumor psoralen + ultraviolet A treatment rates also increased

267 This study's data suggest that psoralen + ultraviolet A-induced p53 mutations may play

268 he development of nonmelanoma skin cancer in psoralen + ultraviolet A-treated patients, but these mut

269 Psoralen + ultraviolet A-treated psoriasis patients appe

270 Psoralen + ultraviolet A-treated psoriasis patients are

271 carcinomas were studied in a cohort of 1380 psoralen + ultraviolet A-treated psoriasis patients pros

272 ultraviolet B for skin cancer development in psoralen + ultraviolet A-treated psoriasis patients.

273 let-type mutations, two (5%) tumors had only psoralen + ultraviolet A-type mutations, and 18 (49%) tu

274 Interestingly, psoralen + ultraviolet A-type p53 mutations were more fr

275 ising in patients with high-dose exposure to psoralen + ultraviolet A.

276 er risk among patients who have discontinued psoralen+ultraviolet A and the risk of a first tumor wit

277 In conclusion, substantial exposure to psoralen+ultraviolet A dramatically increases the risk o

278 ents because the cancer risk associated with psoralen+ultraviolet A is persistent.

279 onmelanoma skin cancer and prior exposure to psoralen+ultraviolet A remains an important issue in the

280 5 years, about 7% of patients with < or =200 psoralen+ultraviolet A treatments and more than half of

281 sk was noted during the first 15 years after psoralen+ultraviolet A was discontinued.

282 Psoralen+ultraviolet A-treated psoriasis patients are at

283 ort of 1,380 psoriasis patients treated with psoralen+ultraviolet A.

284 of which 59.4% were considered years without psoralen+ultraviolet.

285 gulation of CYP2S1 by ultraviolet radiation, psoralen-ultraviolet A (PUVA), and topical drugs used to

286 nventional one-photon excitation, as used in psoralen + UV A radiation (320-400 nm) therapy.

287 In this study, using a psoralen-UV cross-linking method and an in vitro RdRP as

288 ral replication by attenuating the VVVs with psoralen-UV light treatment.

289 Electron microscopy of psoralen/UV cross-linked DNA revealed t-loops in enriche

290 Psoralen/UV photocrosslinking studies revealed that meta

291 Similar data were obtained using psoralen/UVA, signifying that MLH1 influences the cellul

292 ous times after introduction into cells, the psoralen was activated by exposure to long wave ultravio

293 was shown to establish the position at which psoralen was added to the target DNA duplex and determin

294 Psoralen was conjugated to the 5'-end of these clamp-for

295 Gene activation by pso-TFOs in which the psoralen was linked to the TFO via a disulfide bond was

296 The activity of this TFO, linked to psoralen, was measured in a mammalian cell line that was

297 f the 5-propynyl-U change the orientation of psoralen with respect to the upper-strand C.

298 clude topical corticosteroids, phototherapy (psoralen with UVA or UVB), topical chemotherapy, topical

299 o attract TnsC, we show that the location of psoralen within the pyrimidine motif triplex does alter

300 roblasts were incubated with tritium-labeled psoralen without TFO to maximize detectable damage and i