1 ined four proteins on an anti-FABP1 antibody
pulldown.
2 CSNAP (Chemical Similarity Network Analysis
Pulldown),
a new computational target identification met
3 Here we miniaturize the concept of affinity
pulldown,
a gold-standard in vitro PPI interrogation tec
4 ed activation of GEF-H1 and RhoA detected in
pulldown activation assays.
5 Glutathione S-transferase (GST) affinity
pulldowns also suggest that p18 and Gag interact.
6 ransfer (FRET) and glutathione S-transferase
pulldown analyses identified Akt1 pleckstrin homology do
7 This interaction was confirmed with
pulldown analyses in which the GST-PerCter protein selec
8 Immunoprecipitation and
pulldown analyses revealed that Plk3 physically interact
9 yeast 2-hybrid and glutathione-S-transferase
pulldown analyses show Robo4 binding to a Wiskott-Aldric
10 e EGFR by coimmunoprecipitation and affinity
pulldown analyses, and the primarily trans-Golgi network
11 Accordingly,
pulldown analysis and fluorescent FKBP12 binding studies
12 GST
pulldown analysis confirmed that the N terminus region o
13 Immuno-
pulldown analysis from wild-type (WT) mouse tissue showe
14 Pulldown analysis revealed that Cdh1, but not Cdc20, was
15 Pulldown analysis revealed that Plk1 physically interact
16 e globular regions of C1q as demonstrated by
pulldown and cell surface co-localization experiments.
17 Both oligonucleotide
pulldown and chromatin immunoprecipitation assays reveal
18 By oligonucleotide
pulldown and chromatin immunoprecipitation assays, we fo
19 The interaction was confirmed by in vitro
pulldown and co-immunoprecipitation assays and was shown
20 merizations of these three NHERF proteins by
pulldown and co-immunoprecipitation assays.
21 ms a stable complex with DAT in vivo via GST
pulldown and co-immunoprecipitation assays.
22 Pulldown and co-immunoprecipitation experiments identifi
23 n was confirmed by glutathione S-transferase
pulldown and co-immunoprecipitation experiments.
24 The interaction was confirmed by
pulldown and coimmunoprecipitation assays in native cell
25 P1 interaction was confirmed by both peptide
pulldown and coimmunoprecipitation assays, which also de
26 GST
pulldown and coimmunoprecipitation studies reveal that R
27 Using in vitro
pulldown and competition assays, we demonstrate that thi
28 equences was demonstrated by oligonucleotide
pulldown and fluorescence polarization.
29 is an interacting partner of IRSp53 through
pulldown and Forster resonance energy transfer analysis,
30 We show by mutagenesis,
pulldown and hydrogen/deuterium exchange mass spectromet
31 o glutathione S-transferase (GST) E-cadherin
pulldown and immunoblot analysis to assess levels of unc
32 SA-agarose
pulldown and immunoblotting for IRF5 were used to determ
33 Pulldown and immunofluorescence binding assays and surfa
34 Nanog directly binds the FAK protein by
pulldown and immunoprecipitation assays, and proteins co
35 In protein
pulldown and immunoprecipitation experiments, binding of
36 olecular mechanisms were investigated by RNA
pulldown and immunoprecipitation, mass spectrometry, mic
37 racts with ERK1/2 by using both in vitro GST
pulldown and in vivo co-immunoprecipitation assays.
38 Affinity
pulldown and kinase profiling studies implicate Erb3 bin
39 is simple, reusable, and also applicable to
pulldown and kinetic activity/binding assays.
40 By oligonucleotide
pulldown and mass spectrometry discovery approaches, we
41 Using peptide
pulldown and mass spectrometry, we identified angiomotin
42 target of Semapimod using ATP-desthiobiotin
pulldown and mass spectroscopy.
43 We have used
pulldown and peptide array overlay assays to study inter
44 Glutathione S-transferase fusion
pulldown and receptor mutational analyses indicate that
45 In vitro
pulldown and surface plasmon resonance assays, in contra
46 Vav3-Cdc37 interaction was confirmed by GST
pulldown and, for native proteins, by co-immunoprecipita
47 We demonstrate in this paper using GST
pulldowns and coimmunoprecipitation studies that SAP con
48 RNA
pulldowns and costainings show that MyoD mRNA interacts
49 However, glutathione S-transferase
pulldowns and intragenic complementation analysis of sel
50 Pulldowns and isothermal titration calorimetry revealed
51 Further analysis using
pulldowns and size-exclusion chromatography underscored
52 MORF proteins by yeast two-hybrid, in vitro
pulldown,
and bimolecular fluorescence complementation a
53 Promoter analysis, DNA
pulldown,
and electrophoretic mobility shift assays supp
54 FAK could be shown by mammalian two-hybrid,
pulldown,
and far Western studies.
55 munoprecipitation, glutathione S-transferase
pulldown,
and fluorescence polarization assays employing
56 -hybrid screening, glutathione S-transferase
pulldown,
and immunoprecipitation methods.
57 tography, Forster resonance energy transfer,
pulldown,
and in vitro GEF assays to demonstrate that re
58 munoprecipitation, glutathione S-transferase
pulldown,
and luciferase assays show a physical and func
59 l2 as confirmed with co-immunoprecipitation,
pulldown,
and mammalian two-hybrid assays.
60 Immunohistochemical colocalization, GST
pulldown,
and surface plasmon resonance studies revealed
61 Using a novel RNA
pulldown approach that utilized endogenous S1-tagged PIN
62 We used a biotinylated RNA
pulldown approach to isolate host factors binding to the
63 Using a directed RNA
pulldown approach, we identified two components of this
64 Similarly, results from CBX4-BioTAP protein
pulldowns are consistent with reports of a diversity of
65 Interaction was confirmed by a GST
pulldown assay and by coimmunoprecipitation in human H29
66 s was confirmed by glutathione S-transferase
pulldown assay and co-immunoprecipitation assay in human
67 rabidopsis and Nicotiana benthamiana using a
pulldown assay and fluorescence resonance energy transfe
68 em and an in vitro glutathione-S-transferase
pulldown assay and observed interactions between cyclin
69 egion of AKAP79 was able to bind PP1 by both
pulldown assay and surface plasmon resonance.
70 interaction by site-directed mutagenesis and
pulldown assay and thereby confirm that the major bindin
71 s work establishes the single-molecule lipid
pulldown assay as a simple and highly sensitive approach
72 t with pU(L)6 in a glutathione S-transferase
pulldown assay in the absence of other viral proteins an
73 oprecipitation and glutathione S-transferase
pulldown assay in vitro.
74 A RAS-binding domain
pulldown assay indicated that RIT1 A57G and Y89H were hi
75 Pulldown assay of GST-KOPR-C-tail with HA-GEC1 or HA-GAB
76 to embryonic organ explants, with a microRNA
pulldown assay that allows direct identification of micr
77 To address this, we used an in vitro
pulldown assay to define a series of five arginine resid
78 Rosetta pLysS cells, purified, and used in a
pulldown assay to identify interacting proteins from hum
79 A luciferase reporter assay and a
pulldown assay using biotinylated INS-class I VNTR probe
80 V envelope glycoproteins were also used in a
pulldown assay with beads coated with heparin, a close H
81 ctivity enzyme-linked immunosorbent assay, a
pulldown assay, and immunostaining with a monoclonal ant
82 ipt in human T cells and found, using biotin
pulldown assay, that HuR specifically interacts with its
83 In a
pulldown assay, the His-tagged Myb1 interacted with a GS
84 led-coil domain of MuRF1 was demonstrated by
pulldown assay.
85 f apoE, as determined by an in vitro heparin
pulldown assay.
86 Plk1 decreased its affinity for IKKgamma in
pulldown assay.
87 or PP2A-B) using a glutathione S-transferase-
pulldown assay.
88 active state of AIP1 assessed by an in vitro
pulldown assay.
89 d to form a stable complex with SpoIVFB in a
pulldown assay.
90 s by confocal microscopy and in an in vitro "
pulldown"
assay.
91 In vitro
pulldown assays also indicate that DinB(C66A) binds RecA
92 The
pulldown assays also indicated the presence of Cox16p in
93 Furthermore, our results from both GST
pulldown assays and analytical ultracentrifugation show
94 een aldolase and SUR was confirmed using GST
pulldown assays and coimmunoprecipitation assays.
95 Using
pulldown assays and mass spectrometry, we have identifie
96 e in eIF5 interaction with eIF1 and eIF3c in
pulldown assays and reduces the eIF5-mediated stimulatio
97 70-binding site in SOD2, we used a series of
pulldown assays and showed that hsp70 binds to the amino
98 DM2 required for p21(Waf1) degradation using
pulldown assays and Western blotting and then examined t
99 Complementary to this, we applied
pulldown assays as well as microscale thermophoresis as
100 ation analysis, immunoprecipitation, and GST
pulldown assays based on the theoretical predictions rev
101 Size exclusion chromatography and
pulldown assays both indicate that the lower pH conforma
102 We used L-selectin cytoplasmic tail peptide
pulldown assays combined with high sensitivity liquid ch
103 Using
pulldown assays combined with mass spectrometry analysis
104 oprecipitation and glutathione S-transferase
pulldown assays confirm the complex formation between TB
105 Co-immunoprecipitation and
pulldown assays confirmed PKC and beta-catenin as bindin
106 Both coimmunoprecipitation and in vitro
pulldown assays confirmed that ASAP1 directly binds to F
107 Pulldown assays confirmed that the binding between the p
108 Pulldown assays confirmed the presence of newly translat
109 In vitro
pulldown assays confirmed this interaction, which was fo
110 Co-immunoprecipitation and
pulldown assays coupled with site-directed mutagenesis d
111 GST
pulldown assays demonstrate that the dimerization domain
112 Pulldown assays demonstrated interaction between betaCaM
113 erminal kinase domain combined with in vitro
pulldown assays demonstrated that eriodictyol, a flavano
114 Pak-CRIB
pulldown assays demonstrated that Norbin promotes the P-
115 and glutathione S-transferase fusion protein
pulldown assays demonstrated that Rab8 interacted with t
116 RNA
pulldown assays demonstrated that SRSF3 binds to an alte
117 Glutathione S-transferase (GST)
pulldown assays demonstrated that the hnRNP H NLS intera
118 oprecipitation and glutathione S-transferase
pulldown assays demonstrated that the N terminus of ClC-
119 Two-hybrid and
pulldown assays demonstrated that UL20, but no other HSV
120 GST
pulldown assays demonstrated that vIRF1 interacts with U
121 roteins within platelets and confirmation by
pulldown assays followed by immunoblotting, we identifie
122 Pulldown assays from Arabidopsis thaliana tissue culture
123 trometry and glutathione S-transferase (GST)
pulldown assays identified integrin alpha5 as a novel Sc
124 GST
pulldown assays in yeast lysates demonstrated physical i
125 , isothermal titration calorimetry data, and
pulldown assays indicated that CaM-N and CaM-C both can
126 Structural results and
pulldown assays indicated that L3 renders an in-built ge
127 In this study, glutathione S-transferase
pulldown assays indicated that residues 1 to 68 of UL84
128 Pulldown assays of a Orai1-CMBD(W76E) mutant, gel filtra
129 Immunoprecipitation and
pulldown assays of purified proteins demonstrated a dire
130 Finally,
pulldown assays reveal a bipartite physical interaction
131 Co-immunopurification and
pulldown assays reveal that P2X4 receptors complex with
132 Coimmunoprecipitation and in vitro
pulldown assays reveal that phosphorylation of MyoGEF at
133 Glutathione S-transferase
pulldown assays revealed binding of CFTR to alpha-AP-2 (
134 Rho
pulldown assays revealed that Cryptococcus induces activ
135 Pulldown assays revealed that either of CSS2A, CSS2B, an
136 pitation and glutathione S-transferase (GST)
pulldown assays revealed that GBP1 interacted with the N
137 Yeast two-hybrid and direct
pulldown assays revealed that the N-terminal domain of t
138 Coimmunoprecipitation and
pulldown assays revealed that vimentin interacted with A
139 Results from
pulldown assays show that ARF6 exchanges GDP for GTP in
140 Both in vitro and in vivo
pulldown assays show that MyoGEF interacts with CSPP.
141 r hemin exporter, results with hemin-agarose
pulldown assays showed that Abc3 binds to hemin.
142 Oligo
pulldown assays showed that binding of Myc to the Inr el
143 Pulldown assays showed that NS2 forms complexes with bot
144 by absorbance spectroscopy and hemin-agarose
pulldown assays showed that Shu1 interacts with hemin, w
145 In vitro and in vivo
pulldown assays showed that the carboxyl-terminal region
146 RNA
pulldown assays showed that UL84 interacted with IRS1 mR
147 In vitro translation and
pulldown assays suggest direct interaction between BCL10
148 Although
pulldown assays suggest that the presence of N- and C-te
149 Here, we show by yeast two-hybrid and
pulldown assays that SpoVID also interacts directly with
150 selected biochemical pathways; (c) affinity
pulldown assays that, in some cases, confirm and even ex
151 al mRNA-binding proteins identified from RNA
pulldown assays to determine which of these exhibit bona
152 Here, we used biolayer interferometry and
pulldown assays to identify regions of RAG1 necessary fo
153 Using G protein activity and in vitro
pulldown assays we demonstrate that G alpha(i3) is a bet
154 depending on its CTD phosphorylation state,
pulldown assays were performed using the CTD of the duck
155 tial effect, glutathione S-transferase (GST)
pulldown assays were performed, revealing that Y544 is c
156 However, our enzyme
pulldown assays with different polymeric substrates sugg
157 o-immunoprecipitation, two-hybrid assay, and
pulldown assays with expressed proteins.
158 For the active residues, we performed
pulldown assays with membrane-impermeable 2-aminoethyl m
159 o-immunoprecipitation with ACCA antibody and
pulldown assays with recombinant AKR1B10 protein.
160 ed both in vitro by Far-Western and antibody
pulldown assays with recombinant proteins and in vivo by
161 say that combines principles of conventional
pulldown assays with single-molecule fluorescence micros
162 Here, using size-exclusion chromatography,
pulldown assays, and small angle x-ray scattering, we sh
163 brid mating and co-transformation protocols,
pulldown assays, and surface plasmon resonance analysis.
164 In
pulldown assays, CR binding to fusion proteins containin
165 biophysical methods, including heterocomplex
pulldown assays, far-UV CD spectroscopy, the thioflavin
166 ombination of kindlin knockdown, biochemical
pulldown assays, fluorescence microscopy, fluorescence r
167 oplasmic capping complex was demonstrated by
pulldown assays, gel filtration and proximity-dependent
168 Pulldown assays, gel filtration, isothermal titration ca
169 As shown with affinity
pulldown assays, PrgJ and the K471E mutant protein inter
170 In immunoprecipitation and
pulldown assays, ShcA, via its SH2 domain, was associate
171 GST-VCP/p97 bound purified PP2A in
pulldown assays, showing direct protein-protein interact
172 acted with the helicase domain of BKV Tag in
pulldown assays, suggesting that NFI helps recruit Tag t
173 By GST
pulldown assays, the interaction domains between HMG2L1
174 In
pulldown assays, the rank order of AnkG binding strength
175 g luciferase p-miR-Report constructs and RNA
pulldown assays, we confirmed that miR-511 bound directl
176 Using
pulldown assays, we demonstrate that SIRT1-Delta2/9 bind
177 Using EMSA, supershift assays, and promoter
pulldown assays, we demonstrated that CREB, ATF-2, and c
178 By
pulldown assays, we discovered that in addition to the p
179 Furthermore, using
pulldown assays, we discovered that Sam68 is a possible
180 ce energy transfer experiments, and in vitro
pulldown assays, we have now identified the key residues
181 Using the human Cad11 cytoplasmic domain in
pulldown assays, we identified human angiomotin (Amot),
182 pitation (co-IP), mass spectrometry, and GST
pulldown assays, we identified poly(ADP-ribose) polymera
183 assays, and glutathione S-transferase (GST)
pulldown assays, we show that NR2A subunits interact dir
184 We next performed
pulldown assays, with GGGGCC5, in conjunction with mass
185 by using RNA immunoprecipitation and biotin
pulldown assays.
186 cence, flow cytometry, real-time RT-PCR, and
pulldown assays.
187 co-immunoprecipitation and in vitro protein
pulldown assays.
188 d as the 14-3-3 binding region by GST-14-3-3
pulldown assays.
189 d the TM 4,5-loop was demonstrated using GST
pulldown assays.
190 1 for binding to both PP2Acalpha isoforms in
pulldown assays.
191 th E2 and interacted only weakly with NS3 in
pulldown assays.
192 n protein (amino acids 475-589) on liposomal
pulldown assays.
193 ently confirmed by glutathione S-transferase
pulldown assays.
194 nal domain of RPW8.2, which was confirmed by
pulldown assays.
195 oprecipitation and glutathione S-transferase
pulldown assays.
196 tion with Akt2 by co-immunoprecipitation and
pulldown assays.
197 al 44 amino acids of PDZD11, as shown by GST-
pulldown assays.
198 h phosphatase abolishes their association in
pulldown assays.
199 and in vitro glutathione S-transferase (GST)
pulldown assays.
200 otein complex immunoprecipitation and biotin
pulldown assays.
201 ), as shown by yeast two-hybrid and in vitro
pulldown assays.
202 Using
pulldown binding assays, we find the alpha-spectrin EF-d
203 d -2 with recombinant integrin beta tails in
pulldown binding assays.
204 The interaction was confirmed by GST
pulldown,
blot overlay, and co-immunoprecipitation assay
205 ng endosome compartments were seen following
pulldown by immunoaffinity chromatography with Rab-speci
206 n was confirmed by glutathione S-transferase
pulldown,
coimmunoprecipitation, and laser confocal micr
207 this stem-loop region using an RNA affinity
pulldown-
coupled mass spectrometry approach and identifi
208 T84D enhances the ability of origin ssDNA to
pulldown Dpb11, and Sld2 binding to origin ssDNA may be
209 reated samples, from which we also find that
pulldown efficiency sharply increases for DNA fragments
210 found, although the corresponding biases in
pulldown efficiency were all <5%.
211 equence specificity of MBD2-DNA binding in a
pulldown experiment revealing three potential biases in
212 third protein in yeast three-hybrid assays,
pulldown experiments (luminescence-based mammalian inter
213 Pulldown experiments also indicated that all four core p
214 Pulldown experiments and chemical shift perturbation ana
215 According to
pulldown experiments and in vitro binding assays, Cspalp
216 However, the target was identified based on
pulldown experiments and in vitro binding data, without
217 Pulldown experiments confirmed these results, as HMGB1 w
218 Yeast two-hybrid, co-immunoprecipitation and
pulldown experiments demonstrate Piasy and Pias1 interac
219 Single-molecule
pulldown experiments demonstrate that each molecule of O
220 MBP
pulldown experiments demonstrated that the UreD domain o
221 Chromatin immunoprecipitation and oligo
pulldown experiments demonstrated that whereas binding o
222 ROMK antibody and glutathione S-transferase
pulldown experiments demonstrated the association betwee
223 By RNA
pulldown experiments followed by MALDI/TOF-MS analysis,
224 Pulldown experiments from adult parasite culture medium
225 Immunoprecipitation and
pulldown experiments indicated that Tpr2 associates with
226 Co-immunoprecipitation and GST
pulldown experiments provided evidence that EPLIN intera
227 Glutathione S-transferase
pulldown experiments revealed a direct interaction betwe
228 Mutagenesis and
pulldown experiments revealed multiple Hsp70-binding sit
229 Pulldown experiments revealed that it is not stably asso
230 and glutathione S-transferase fusion protein
pulldown experiments show that tyrosol-phosphorylated Er
231 Coimmunoprecipitation and biotin
pulldown experiments showed that GR associates with CCL2
232 Pulldown experiments showed that the hsp90-iNOS complex
233 HCV
pulldown experiments showed that this phenomenon was cau
234 Glutathione S-transferase
pulldown experiments showed there was a direct physical
235 ological measurements in Xenopus oocytes and
pulldown experiments to analyze the direct interaction b
236 Pulldown experiments using affinity-tagged Spx showed th
237 Reciprocal glutathione-S-transferase (GST)
pulldown experiments using bacterially expressed UL44 an
238 Pulldown experiments using extracts of B. subtilis cells
239 his is a direct interaction, demonstrated by
pulldown experiments using purified proteins.
240 unoprecipitation and AQP0 C-terminal peptide
pulldown experiments were used to confirm the protein-pr
241 ial ligand lipopolysaccharide and subsequent
pulldown experiments with biotin-avidin affinity chromat
242 We performed
pulldown experiments with biotinylated thymosin beta-4 (
243 Using immunoprecipitation and
pulldown experiments with domain-specific recombinant fr
244 eins interacting with GlyRbeta, we performed
pulldown experiments with rat brain extracts using a glu
245 wo proteins using co-immunoprecipitation and
pulldown experiments with truncated or mutant Drosophila
246 trast, results from kinetic studies, heparin
pulldown experiments, and inhibition experiments with an
247 analysis, immunoprecipitations, mutagenesis,
pulldown experiments, and peptide arrays constrained PP1
248 Using glutathione S-transferase
pulldown experiments, chemical cross-linking, size exclu
249 (v) According to 20 S proteasome
pulldown experiments, Hsp60 is physically associated wit
250 In
pulldown experiments, PC1 bound to Galpha(12), but not t
251 R1 constructs, and glutathione S-transferase
pulldown experiments, we demonstrate that TRAF2 rapidly
252 -tagged in vivo and used as bait in separate
pulldown experiments.
253 unoprecipitation and AQP0 C-terminal peptide
pulldown experiments.
254 embled the GAP complex in label transfer and
pulldown experiments.
255 f 1 (1-Btn) to be used as a tool in affinity
pulldown experiments.
256 GST "
pulldown"
experiments demonstrated that the Ptp52F subst
257 PAR-resin
pulldown,
followed by proteomic analysis, demonstrated h
258 le length regulators were identified in EML1
pulldowns from embryonic brain extracts.
259 This is confirmed by comparing input versus
pulldown high-throughput sequencing data on M.SssI-treat
260 tiple studies in vitro and in vivo including
pulldown,
immunoprecipitation and chromatin immunoprecip
261 as shown using fluorescence energy transfer,
pulldown,
immunoprecipitation, cross-linking experiments
262 a was confirmed by glutathione S-transferase
pulldown in vitro and coimmunoprecipitation in vivo.
263 o anti-ubiquitin were seen in the optineurin
pulldown,
indicating that optineurin was ubiquitinated.
264 metal cofactor bound to LpxC, we developed a
pulldown method to rapidly purify tagged LpxC under anae
265 pled to a green fluorescent protein-nanotrap
pulldown methodology and liquid chromatography-tandem ma
266 Cq-IGFBP) protein was produced and, using a "
pulldown"
methodology, was shown to specifically interac
267 Protein
pulldown,
molecular docking, molecular dynamics simulati
268 nterrogation technique, to perform nanoscale
pulldowns (
NanoSPDs) within living cells.
269 Pulldown of active Rap1 and fluorescence microscopic ana
270 Pulldown of ectopically expressed Cul1, the scaffold pro
271 re, we used immunoprecipitation and affinity
pulldown of ectopically expressed p30 coupled with mass
272 ircPVT1, only let-7 was found enriched after
pulldown of endogenous CircPVT1, suggesting that CircPVT
273 +), Ni(2+), Ag(+), Hg(2+), and Bi(3+) blocks
pulldown of MTF1(321-675) by PAO beads in vitro and in v
274 Using affinity
pulldowns of Strep-tagged UAPs from Arabidopsis and rice
275 au interaction cluster that contained 33 Tau
pulldown proteins.
276 biological approaches, including active RAS
pulldown,
reporter and Comet assays, small interfering R
277 on of a polyhistidine-tagged Pup followed by
pulldown revealed that a broad spectrum of proteins were
278 Moreover, affinity
pulldowns show that p18 and the CTR interact.
279 GST
pulldown showed that TAT-SNAP-23 bound to the combinatio
280 We use the single-molecule
pulldown (
SiMPull) assay that combines principles of con
281 By means of single-molecule
pulldown (
SiMPull), we determined a TAP/tapasin ratio of
282 Using poly(A)
pulldown stranded RNA-seq and a 3' end transcript counti
283 Here, we use a novel RNA
pulldown strategy coupled with mass spectrometry to iden
284 munoprecipitation and biotin-labeled miR-665
pulldown studies identified Kat6a as another potential t
285 Vesicle
pulldown studies showed that acidic phospholipids recrui
286 unoprecipitated from T cell lysates, and GST-
pulldown studies showed that Itk's Src homology 2 domain
287 Using colocalization and
pulldown studies we further document a noggin-insensitiv
288 t signaling that interacts with the VDR, GST
pulldown studies were performed.
289 oprecipitation and glutathione S-transferase
pulldown studies.
290 ble proteins were studied by a precipitation
pulldown technique.
291 ly to purified 14-3-3zeta as demonstrated by
pulldown techniques.
292 ed animals demonstrated stronger acute viral
pulldown than controls, but a trend for higher acute vir
293 , we have utilized glutathione S-transferase
pulldowns,
two-hybrid analysis, and NMR to demonstrate t
294 is interaction was confirmed by a reciprocal
pulldown using FLAG-tagged Ycf54 as bait.
295 immunoprecipitations from heart extracts and
pulldowns using heterologously expressed proteins provid
296 Biotin-RNA
pulldown,
UV-crosslinking and gel shift experiments indi
297 Using
pulldowns,
we here identify teneurins, type II transmemb
298 By using glutathione S-transferase (GST)
pulldowns,
we identified an essential role of lysine 343
299 density gradient centrifugation and antibody
pulldowns,
we show that all six A subunits are associate
300 Protein
pulldowns were used to identify Fam65b-interacting prote